Margaret Wheelock

Summary

Affiliation: University of Nebraska Medical Center
Country: USA

Publications

  1. pmc Collagen I-mediated up-regulation of N-cadherin requires cooperative signals from integrins and discoidin domain receptor 1
    Yasushi Shintani
    Department of Oral Biology, University of Nebraska Medical Center, Omaha, NE 68198, USA
    J Cell Biol 180:1277-89. 2008
  2. doi request reprint Cadherin switching
    Margaret J Wheelock
    University of Nebraska Medical Center, Department of Oral Biology and Eppley Cancer Center, Omaha, NE 68198 7696, USA
    J Cell Sci 121:727-35. 2008
  3. ncbi request reprint Cadherin-mediated cellular signaling
    Margaret J Wheelock
    University of Nebraska Medical Center, 987696 Nebraska Medical Center, Omaha, NE 68198 7696, USA
    Curr Opin Cell Biol 15:509-14. 2003
  4. ncbi request reprint Cadherins as modulators of cellular phenotype
    Margaret J Wheelock
    Department of Oral Biology, College of Dentistry and Eppley Cancer Center, University of Nebraska Medical Center, Omaha, Nebraska 68198 7696, USA
    Annu Rev Cell Dev Biol 19:207-35. 2003
  5. ncbi request reprint Characterization of cadherin-24, a novel alternatively spliced type II cadherin
    Bryan J Katafiasz
    University of Nebraska Medical Center, Department of Oral Biology, College of Dentistry and Eppley Cancer Center, Omaha, Nebraska 68198, USA
    J Biol Chem 278:27513-9. 2003
  6. pmc The regulatory or phosphorylation domain of p120 catenin controls E-cadherin dynamics at the plasma membrane
    Yuri Fukumoto
    Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, Nebraska 68198 7696, USA
    Exp Cell Res 314:52-67. 2008
  7. pmc E-cadherin/catenin complexes are formed cotranslationally in the endoplasmic reticulum/Golgi compartments
    Matthew W Curtis
    Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, Nebraska 68198 7696, USA
    Cell Commun Adhes 15:365-78. 2008
  8. ncbi request reprint Differential displacement of classical cadherins by VE-cadherin
    Meena Jaggi
    Department of Oral Biology and Eppley Institute for Cancer Research, University of Nebraska Medical Center, Omaha, NE 68198 7696, USA
    Cell Commun Adhes 9:103-15. 2002
  9. pmc NHERF links the N-cadherin/catenin complex to the platelet-derived growth factor receptor to modulate the actin cytoskeleton and regulate cell motility
    Christopher S Theisen
    Department of Biochemistry and Molecular Biology, Eppley Cancer Center, University of Nebraska Medical Center, Omaha, NE 68198 7696, USA
    Mol Biol Cell 18:1220-32. 2007
  10. ncbi request reprint Cadherin switching: essential for behavioral but not morphological changes during an epithelium-to-mesenchyme transition
    Masato Maeda
    Department of Oral Biology College of Dentistry, University of Nebraska Medical Center, Omaha, NE 68198 7696, USA
    J Cell Sci 118:873-87. 2005

Research Grants

  1. ASPECTS OF CADHERIN/CATENIN COMPLEXES
    Margaret Wheelock; Fiscal Year: 2001
  2. Nebraska Center for Cellular Signaling
    Margaret Wheelock; Fiscal Year: 2007
  3. ASPECTS OF CADHERIN/CATENIN COMPLEXES
    Margaret Wheelock; Fiscal Year: 2007
  4. ASPECTS OF CADHERIN/CATENIN COMPLEXES
    Margaret Wheelock; Fiscal Year: 2005

Collaborators

Detail Information

Publications31

  1. pmc Collagen I-mediated up-regulation of N-cadherin requires cooperative signals from integrins and discoidin domain receptor 1
    Yasushi Shintani
    Department of Oral Biology, University of Nebraska Medical Center, Omaha, NE 68198, USA
    J Cell Biol 180:1277-89. 2008
    ..Both receptor complexes rely on the p130 Crk-associated substrate scaffold. Interestingly, Rap1, but not Rho family guanosine triphosphatases, is required for the response to collagen I...
  2. doi request reprint Cadherin switching
    Margaret J Wheelock
    University of Nebraska Medical Center, Department of Oral Biology and Eppley Cancer Center, Omaha, NE 68198 7696, USA
    J Cell Sci 121:727-35. 2008
    ..In addition, p120-catenin probably plays multiple roles in cadherin switching, regulating Rho GTPases and stabilizing cadherins...
  3. ncbi request reprint Cadherin-mediated cellular signaling
    Margaret J Wheelock
    University of Nebraska Medical Center, 987696 Nebraska Medical Center, Omaha, NE 68198 7696, USA
    Curr Opin Cell Biol 15:509-14. 2003
    ....
  4. ncbi request reprint Cadherins as modulators of cellular phenotype
    Margaret J Wheelock
    Department of Oral Biology, College of Dentistry and Eppley Cancer Center, University of Nebraska Medical Center, Omaha, Nebraska 68198 7696, USA
    Annu Rev Cell Dev Biol 19:207-35. 2003
    ....
  5. ncbi request reprint Characterization of cadherin-24, a novel alternatively spliced type II cadherin
    Bryan J Katafiasz
    University of Nebraska Medical Center, Department of Oral Biology, College of Dentistry and Eppley Cancer Center, Omaha, Nebraska 68198, USA
    J Biol Chem 278:27513-9. 2003
    ..In addition, aggregation assays show that both forms of cadherin-24 mediate strong cell-cell adhesion...
  6. pmc The regulatory or phosphorylation domain of p120 catenin controls E-cadherin dynamics at the plasma membrane
    Yuri Fukumoto
    Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, Nebraska 68198 7696, USA
    Exp Cell Res 314:52-67. 2008
    ..These data suggest that serine/threonine phosphorylation of p120 influences the dynamics of E-cadherin in junctions...
  7. pmc E-cadherin/catenin complexes are formed cotranslationally in the endoplasmic reticulum/Golgi compartments
    Matthew W Curtis
    Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, Nebraska 68198 7696, USA
    Cell Commun Adhes 15:365-78. 2008
    ..To investigate this, the authors generated an antibody against the proregion of E-cadherin and have used it to examine the assembly of the E-cadherin/catenin complex...
  8. ncbi request reprint Differential displacement of classical cadherins by VE-cadherin
    Meena Jaggi
    Department of Oral Biology and Eppley Institute for Cancer Research, University of Nebraska Medical Center, Omaha, NE 68198 7696, USA
    Cell Commun Adhes 9:103-15. 2002
    ..Our data suggest that VE-cadherin specifically influences the cellular localization of N-cadherin, independent of cell type, and does not effect the localization of other classical cadherins...
  9. pmc NHERF links the N-cadherin/catenin complex to the platelet-derived growth factor receptor to modulate the actin cytoskeleton and regulate cell motility
    Christopher S Theisen
    Department of Biochemistry and Molecular Biology, Eppley Cancer Center, University of Nebraska Medical Center, Omaha, NE 68198 7696, USA
    Mol Biol Cell 18:1220-32. 2007
    ..These studies implicate N-cadherin and beta-catenin in cell migration via PDGF-R-mediated signaling through the scaffolding molecule NHERF...
  10. ncbi request reprint Cadherin switching: essential for behavioral but not morphological changes during an epithelium-to-mesenchyme transition
    Masato Maeda
    Department of Oral Biology College of Dentistry, University of Nebraska Medical Center, Omaha, NE 68198 7696, USA
    J Cell Sci 118:873-87. 2005
    ..Together, these data suggest that cadherin switching is necessary for increased motility but is not required for the morphological changes that accompany EMT...
  11. ncbi request reprint Modulating the strength of cadherin adhesion: evidence for a novel adhesion complex
    Young J Kim
    University of Nebraska Medical Center, College of Dentistry, 769605 Nebraska Medical Center, Omaha, NE 68583, USA
    J Cell Sci 118:3883-94. 2005
    ..Based on our results we propose that a vimentin-based classical cadherin complex functions together with the actin-based complex to promote strong cell-cell adhesion in fibroblasts...
  12. pmc E-cadherin differentially regulates the assembly of Connexin43 and Connexin32 into gap junctions in human squamous carcinoma cells
    Souvik Chakraborty
    Department of Biochemistry and Molecular Biology, Eppley Institute for Research in Cancer and Allied Diseases, Eppley Cancer Center, University of Nebraska Medical Center, Omaha, Nebraska 68198, USA
    J Biol Chem 285:10761-76. 2010
    ....
  13. ncbi request reprint N-cadherin-catenin complexes form prior to cleavage of the proregion and transport to the plasma membrane
    James K Wahl
    University of Nebraska Medical Center, College of Dentistry and Eppley Cancer Center, Omaha, Nebraska 68198 7696, USA
    J Biol Chem 278:17269-76. 2003
    ..These data suggest that a core N-cadherin-catenin complex assembles in the endoplasmic reticulum or Golgi compartment and is transported to the plasma membrane where linkage to the actin cytoskeleton can be established...
  14. ncbi request reprint R-cadherin influences cell motility via Rho family GTPases
    Emhonta Johnson
    Department of Oral Biology, College of Dentistry, Eppley Institute for Research in Cancer and Allied Diseases, and Eppley Cancer Center, University of Nebraska Medical Center, Omaha, Nebraska 68198, USA
    J Biol Chem 279:31041-9. 2004
    ..In addition, we show that R-cadherin induces cell motility when expressed in epithelial cells and that this increased motility is dependent upon Rho GTPase activity...
  15. ncbi request reprint Impaired trafficking of connexins in androgen-independent human prostate cancer cell lines and its mitigation by alpha-catenin
    Rajgopal Govindarajan
    Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, NE 68198, USA
    J Biol Chem 277:50087-97. 2002
    ..Our results suggest that impaired trafficking, and not the inability to form gap junctions, is the major cause of communication deficiency in human prostate cancer cell lines...
  16. pmc Expression artifact with retroviral vectors based on pBMN
    Yoshitaka Fukunaga
    Department of Oral Biology, University of Nebraska Medical Center, Omaha, 68198, USA
    Anal Biochem 395:49-53. 2009
    ..We found that the publicly available sequence of the vector we used does not agree with the experimental sequence. We caution other investigators to be aware of this potential artifact...
  17. ncbi request reprint Collagen I promotes metastasis in pancreatic cancer by activating c-Jun NH(2)-terminal kinase 1 and up-regulating N-cadherin expression
    Yasushi Shintani
    Department of Oral Biology, University of Nebraska Medical Center, Omaha, Nebraska 68198, USA
    Cancer Res 66:11745-53. 2006
    ..We show that JNK1 is activated in response to collagen I, which increases tumorigenesis by up-regulating N-cadherin expression and by increasing motility...
  18. pmc Generation and characterization of monoclonal antibodies against the proregion of human desmoglein-2
    Sarah A Keim
    University of Nebraska Medical Center, Department of Genetics, Cell Biology and Anatomy, Omaha, Nebraska 68198 7696, USA
    Hybridoma (Larchmt) 27:249-58. 2008
    ..In this study, we produced and characterized a number of monoclonal antibodies against the proregion of the desmosomal cadherin, human desmoglein-2, that will facilitate investigations into the processing of this protein...
  19. ncbi request reprint Evidence that the V832M E-cadherin germ-line missense mutation does not influence the affinity of alpha -catenin for the cadherin/catenin complex
    Matthew W Curtis
    University of Nebraska Medical Center, Department of Pathology and Microbiology, Omaha, Nebraska, USA
    Cell Commun Adhes 14:45-55. 2007
    ....
  20. ncbi request reprint Nuclear association of the cytoplasmic tail of MUC1 and beta-catenin
    Yunfei Wen
    Eppley Institute for Research in Cancer and Allied Diseases, Omaha, Nebraska 68198 6805, USA
    J Biol Chem 278:38029-39. 2003
    ....
  21. ncbi request reprint Src activation is not necessary for transforming growth factor (TGF)-beta-mediated epithelial to mesenchymal transitions (EMT) in mammary epithelial cells. PP1 directly inhibits TGF-beta receptors I and II
    Masato Maeda
    Department of Oral Biology, College of Dentistry, University of Nebraska Medical Center, Nebraska Medical Center, Omaha 68198 7696, USA
    J Biol Chem 281:59-68. 2006
    ..Taken together, our data indicated that PP1 interferes with TGF-beta1-mediated EMT not by inhibiting Src family kinases but by inhibiting the Smad pathway via a direct inhibition of TGF-beta receptor kinase activity...
  22. doi request reprint TGF-beta induces formation of F-actin cores and matrix degradation in human breast cancer cells via distinct signaling pathways
    Shyamali Mandal
    Department of Biochemistry, University of Nebraska Medical Center, Omaha, NE 68198 7696, USA
    Exp Cell Res 314:3478-93. 2008
    ....
  23. ncbi request reprint E-cadherin phosphorylation by protein kinase D1/protein kinase C{mu} is associated with altered cellular aggregation and motility in prostate cancer
    Meena Jaggi
    Urological Surgery, University of Nebraska Medical Center, Omaha, NE 68198, USA
    Cancer Res 65:483-92. 2005
    ..Because both E-cadherin and PKD1 are known to be dysregulated in prostate cancer, our study identified an important protein-protein interaction influencing the signal transduction system associated with cell adhesion in prostate cancer...
  24. ncbi request reprint ADH-1 suppresses N-cadherin-dependent pancreatic cancer progression
    Yasushi Shintani
    Department of Oral Biology, University of Nebraska Medical Center, Omaha, NE 68198 7696, USA
    Int J Cancer 122:71-7. 2008
    ....
  25. pmc Phosphoinositide-3 kinase-Rac1-c-Jun NH2-terminal kinase signaling mediates collagen I-induced cell scattering and up-regulation of N-cadherin expression in mouse mammary epithelial cells
    Yasushi Shintani
    Department of Oral Biology, Eppley Institute for Research in Cancer and Allied Diseases, and Eppley Cancer Center, University of Nebraska Medical Center, Omaha, NE 68198 7696, USA
    Mol Biol Cell 17:2963-75. 2006
    ..In addition, we showed that cord formation and branching in three-dimensional culture (EMT-dependent events) required N-cadherin expression and PI3K-Rac1-JNK signaling...
  26. ncbi request reprint N-cadherin-mediated cell motility requires cis dimers
    Young J Kim
    Department of Oral Biology, College of Dentistry, University of Nebraska Medical Center, Omaha, Nebraska, USA
    Cell Commun Adhes 12:23-39. 2005
    ..Here we show that N-cadherin cis dimers are necessary to induce cell motility in epithelial cells and that N-cadherin's ability to modulate the steady state levels of activated small GTPases requires both cis and trans dimers...
  27. pmc Collagen I promotes epithelial-to-mesenchymal transition in lung cancer cells via transforming growth factor-beta signaling
    Yasushi Shintani
    Department of Oral Biology, Eppley Cancer Center, University of Nebraska Medical Center, Omaha, Nebraska, USA
    Am J Respir Cell Mol Biol 38:95-104. 2008
    ..Thus, our data are consistent with the hypothesis that collagen I induces EMT in lung cancer cells by activating autocrine TGF-beta3 signaling. Epidermal growth factor also seems to initiate EMT via a TGF-dependent mechanism...
  28. ncbi request reprint Effect of N-cadherin misexpression by the mammary epithelium in mice
    Karen A Knudsen
    Lankenau Institute for Medical Research, Wynnewood, Pennsylvania 19096, USA
    J Cell Biochem 95:1093-107. 2005
    ..Tumors developed in +/neu and N-cadherin/neu mice, although few tumors in bitransgenic mice expressed N-cadherin, and they did not differ from N-cadherin-negative tumors...
  29. ncbi request reprint Expression of E-cadherin, P-cadherin and N-cadherin in oral squamous cell carcinoma: correlation with the clinicopathologic features and patient outcome
    Sung Woon Pyo
    Department of Oral and Maxillofacial Surgery and Dentistry, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea
    J Craniomaxillofac Surg 35:1-9. 2007
    ..The interaction between the cadherins was also investigated...
  30. ncbi request reprint Cadherins and the mammary gland
    Karen A Knudsen
    Lankenau Institute for Medical Research, Wynnewood, PA 19096, USA
    J Cell Biochem 95:488-96. 2005
    ..Additional research is needed to more precisely define the roles of both classical and desmosomal cadherins and their downstream signaling events, in the development and malignant behavior of breast cancers...
  31. ncbi request reprint Functional allelic loss detected at the protein level in archival human tumours using allele-specific E-cadherin monoclonal antibodies
    Karl Friedrich Becker
    Technische Universitat, Klinikum rechts der Isar, Institut fur Pathologie, Trogerstrasse 18, D 81675 Munchen, Germany
    J Pathol 197:567-74. 2002
    ..For immunohistochemical analysis of E-cadherin, at least two different monoclonal antibodies should be used to exclude alterations of the epitopes resulting in failure to detect a mutant protein...

Research Grants13

  1. ASPECTS OF CADHERIN/CATENIN COMPLEXES
    Margaret Wheelock; Fiscal Year: 2001
    ....
  2. Nebraska Center for Cellular Signaling
    Margaret Wheelock; Fiscal Year: 2007
    ..In addition, the collaborative environment resulting from the formation of the Center will enhance the research infrastructure within Nebraska's Dental Schools. Thus, the chances we will meet all our goals are very high. ..
  3. ASPECTS OF CADHERIN/CATENIN COMPLEXES
    Margaret Wheelock; Fiscal Year: 2007
    ....
  4. ASPECTS OF CADHERIN/CATENIN COMPLEXES
    Margaret Wheelock; Fiscal Year: 2005
    ..Such reagents might be useful to prevent metastasis of tumor cells and/or angiogenesis of endothelial cells. ..