Janet Westpheling

Summary

Affiliation: University of Georgia
Country: USA

Publications

  1. pmc Metabolic engineering of Caldicellulosiruptor bescii yields increased hydrogen production from lignocellulosic biomass
    Minseok Cha
    Department of Genetics, University of Georgia, Athens, GA 30602, USA
    Biotechnol Biofuels 6:85. 2013
  2. pmc Overcoming restriction as a barrier to DNA transformation in Caldicellulosiruptor species results in efficient marker replacement
    Daehwan Chung
    Department of Genetics, University of Georgia, Athens, GA 30602, USA
    Biotechnol Biofuels 6:82. 2013
  3. pmc An integrative expression vector for Actinosynnema pretiosum
    Shan Goh
    Bioprocessing Technology Institute, 20 Biopolis Way, Centros 06 01, Singapore
    BMC Biotechnol 7:72. 2007
  4. pmc Recombinogenic properties of Pyrococcus furiosus strain COM1 enable rapid selection of targeted mutants
    Joel Farkas
    Department of Genetics, University of Georgia, Athens, USA
    Appl Environ Microbiol 78:4669-76. 2012
  5. pmc Methylation by a unique α-class N4-cytosine methyltransferase is required for DNA transformation of Caldicellulosiruptor bescii DSM6725
    Daehwan Chung
    Department of Genetics, University of Georgia, Athens, Georgia, United States of America
    PLoS ONE 7:e43844. 2012
  6. doi request reprint Improved growth media and culture techniques for genetic analysis and assessment of biomass utilization by Caldicellulosiruptor bescii
    Joel Farkas
    Department of Genetics, University of Georgia, Athens, GA 30602, USA
    J Ind Microbiol Biotechnol 40:41-9. 2013
  7. pmc Construction of a stable replicating shuttle vector for Caldicellulosiruptor species: use for extending genetic methodologies to other members of this genus
    Daehwan Chung
    Department of Genetics, University of Georgia, Athens, Georgia, United States of America
    PLoS ONE 8:e62881. 2013
  8. pmc Deletion strains reveal metabolic roles for key elemental sulfur-responsive proteins in Pyrococcus furiosus
    Stephanie L Bridger
    Department of Biochemistry and Molecular Biology, Life Sciences Bldg, University of Georgia, Athens, GA 30602 7229, USA
    J Bacteriol 193:6498-504. 2011
  9. doi request reprint Detection of a novel active transposable element in Caldicellulosiruptor hydrothermalis and a new search for elements in this genus
    Daehwan Chung
    Department of Genetics, University of Georgia, Athens, GA, 30602, USA
    J Ind Microbiol Biotechnol 40:517-21. 2013
  10. pmc Defining components of the chromosomal origin of replication of the hyperthermophilic archaeon Pyrococcus furiosus needed for construction of a stable replicating shuttle vector
    Joel Farkas
    Department of Genetics, University of Georgia, Athens, GA 30602, USA
    Appl Environ Microbiol 77:6343-9. 2011

Collaborators

  • Daehwan Chung
  • Joel Farkas
  • Minseok Cha
  • Michael W W Adams
  • Gina L Lipscomb
  • Jennifer R Huddleston
  • Robert A Scott
  • Karen Stirrett
  • Stephanie L Bridger
  • Dae hwan Chung
  • Shan Goh
  • Jennifer Copeland
  • Philip Grayeski
  • James G Elkins
  • Adam M Guss
  • Estefania Olivar
  • William Nixon
  • Gerrit J Schut
  • Megan Debarry
  • Sonya M Clarkson
  • Megan B Debarry
  • Victor V T Wong
  • Daniel Ng
  • Andrea Camattari
  • Ruth Song
  • Kevin Madden

Detail Information

Publications11

  1. pmc Metabolic engineering of Caldicellulosiruptor bescii yields increased hydrogen production from lignocellulosic biomass
    Minseok Cha
    Department of Genetics, University of Georgia, Athens, GA 30602, USA
    Biotechnol Biofuels 6:85. 2013
    ..Here, we report the first targeted gene deletion, the gene encoding lactate dehydrogenase (ldh), for metabolic engineering of a member of this genus...
  2. pmc Overcoming restriction as a barrier to DNA transformation in Caldicellulosiruptor species results in efficient marker replacement
    Daehwan Chung
    Department of Genetics, University of Georgia, Athens, GA 30602, USA
    Biotechnol Biofuels 6:82. 2013
    ..Metabolic engineering of pathways for product synthesis is relatively simple compared to engineering the ability to utilize non-pretreated biomass...
  3. pmc An integrative expression vector for Actinosynnema pretiosum
    Shan Goh
    Bioprocessing Technology Institute, 20 Biopolis Way, Centros 06 01, Singapore
    BMC Biotechnol 7:72. 2007
    ..pretiosum is lacking. The aim of this study was to construct a vector for heterologous gene expression in A. pretiosum...
  4. pmc Recombinogenic properties of Pyrococcus furiosus strain COM1 enable rapid selection of targeted mutants
    Joel Farkas
    Department of Genetics, University of Georgia, Athens, USA
    Appl Environ Microbiol 78:4669-76. 2012
    ..The ease and speed of this procedure will facilitate the construction of strains with multiple genetic changes and allow the construction of mutants with deletions of virtually any nonessential gene...
  5. pmc Methylation by a unique α-class N4-cytosine methyltransferase is required for DNA transformation of Caldicellulosiruptor bescii DSM6725
    Daehwan Chung
    Department of Genetics, University of Georgia, Athens, Georgia, United States of America
    PLoS ONE 7:e43844. 2012
    ....
  6. doi request reprint Improved growth media and culture techniques for genetic analysis and assessment of biomass utilization by Caldicellulosiruptor bescii
    Joel Farkas
    Department of Genetics, University of Georgia, Athens, GA 30602, USA
    J Ind Microbiol Biotechnol 40:41-9. 2013
    ..These new media represent a significant advance for both genetic manipulation and the study of biomass utilization in C. bescii, and may be applied broadly across the Caldicellulosiruptor genus...
  7. pmc Construction of a stable replicating shuttle vector for Caldicellulosiruptor species: use for extending genetic methodologies to other members of this genus
    Daehwan Chung
    Department of Genetics, University of Georgia, Athens, Georgia, United States of America
    PLoS ONE 8:e62881. 2013
    ..Plasmids containing a carbohydrate binding domain (CBM) and linker region from the C. bescii celA gene were maintained with selection and were structurally stable through transformation and replication in C. bescii and E. coli...
  8. pmc Deletion strains reveal metabolic roles for key elemental sulfur-responsive proteins in Pyrococcus furiosus
    Stephanie L Bridger
    Department of Biochemistry and Molecular Biology, Life Sciences Bldg, University of Georgia, Athens, GA 30602 7229, USA
    J Bacteriol 193:6498-504. 2011
    ..A model is proposed for S(0) reduction by P. furiosus with roles for MBX and NSR in bioenergetics and for SipA in iron-sulfur metabolism...
  9. doi request reprint Detection of a novel active transposable element in Caldicellulosiruptor hydrothermalis and a new search for elements in this genus
    Daehwan Chung
    Department of Genetics, University of Georgia, Athens, GA, 30602, USA
    J Ind Microbiol Biotechnol 40:517-21. 2013
    ....
  10. pmc Defining components of the chromosomal origin of replication of the hyperthermophilic archaeon Pyrococcus furiosus needed for construction of a stable replicating shuttle vector
    Joel Farkas
    Department of Genetics, University of Georgia, Athens, GA 30602, USA
    Appl Environ Microbiol 77:6343-9. 2011
    ..furiosus, plasmids based on the P. furiosus chromosomal origin were structurally unchanged after transformation and were stable without selection for more than 100 generations...
  11. doi request reprint Identification and characterization of CbeI, a novel thermostable restriction enzyme from Caldicellulosiruptor bescii DSM 6725 and a member of a new subfamily of HaeIII-like enzymes
    Dae hwan Chung
    Department of Genetics, University of Georgia, Athens, GA 30602, USA
    J Ind Microbiol Biotechnol 38:1867-77. 2011
    ..A phylogenetic analysis based on sequence alignment and conserved motif searches identified features of CbeI distinct from other members of this group and classified CbeI as a member of a novel subfamily of HaeIII-like enzymes...