Janet Westpheling

Summary

Affiliation: University of Georgia
Country: USA

Publications

  1. pmc Construction of a stable replicating shuttle vector for Caldicellulosiruptor species: use for extending genetic methodologies to other members of this genus
    Daehwan Chung
    Department of Genetics, University of Georgia, Athens, Georgia, United States of America
    PLoS ONE 8:e62881. 2013
  2. pmc Methylation by a unique α-class N4-cytosine methyltransferase is required for DNA transformation of Caldicellulosiruptor bescii DSM6725
    Daehwan Chung
    Department of Genetics, University of Georgia, Athens, Georgia, United States of America
    PLoS ONE 7:e43844. 2012
  3. pmc Metabolic engineering of Caldicellulosiruptor bescii yields increased hydrogen production from lignocellulosic biomass
    Minseok Cha
    Department of Genetics, University of Georgia, Athens, GA 30602, USA
    Biotechnol Biofuels 6:85. 2013
  4. pmc Overcoming restriction as a barrier to DNA transformation in Caldicellulosiruptor species results in efficient marker replacement
    Daehwan Chung
    Department of Genetics, University of Georgia, Athens, GA 30602, USA
    Biotechnol Biofuels 6:82. 2013
  5. pmc An integrative expression vector for Actinosynnema pretiosum
    Shan Goh
    Bioprocessing Technology Institute, 20 Biopolis Way, Centros 06 01, Singapore
    BMC Biotechnol 7:72. 2007
  6. pmc Insights into plant biomass conversion from the genome of the anaerobic thermophilic bacterium Caldicellulosiruptor bescii DSM 6725
    Phuongan Dam
    Institute of Bioinformatics, Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602, USA
    Nucleic Acids Res 39:3240-54. 2011
  7. pmc Recombinogenic properties of Pyrococcus furiosus strain COM1 enable rapid selection of targeted mutants
    Joel Farkas
    Department of Genetics, University of Georgia, Athens, USA
    Appl Environ Microbiol 78:4669-76. 2012
  8. pmc Deletion strains reveal metabolic roles for key elemental sulfur-responsive proteins in Pyrococcus furiosus
    Stephanie L Bridger
    Department of Biochemistry and Molecular Biology, Life Sciences Bldg, University of Georgia, Athens, GA 30602 7229, USA
    J Bacteriol 193:6498-504. 2011
  9. doi request reprint Classification of 'Anaerocellum thermophilum' strain DSM 6725 as Caldicellulosiruptor bescii sp. nov
    Sung Jae Yang
    Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia 30602, USA
    Int J Syst Evol Microbiol 60:2011-5. 2010
  10. pmc Defining components of the chromosomal origin of replication of the hyperthermophilic archaeon Pyrococcus furiosus needed for construction of a stable replicating shuttle vector
    Joel Farkas
    Department of Genetics, University of Georgia, Athens, GA 30602, USA
    Appl Environ Microbiol 77:6343-9. 2011

Collaborators

Detail Information

Publications20

  1. pmc Construction of a stable replicating shuttle vector for Caldicellulosiruptor species: use for extending genetic methodologies to other members of this genus
    Daehwan Chung
    Department of Genetics, University of Georgia, Athens, Georgia, United States of America
    PLoS ONE 8:e62881. 2013
    ..Plasmids containing a carbohydrate binding domain (CBM) and linker region from the C. bescii celA gene were maintained with selection and were structurally stable through transformation and replication in C. bescii and E. coli...
  2. pmc Methylation by a unique α-class N4-cytosine methyltransferase is required for DNA transformation of Caldicellulosiruptor bescii DSM6725
    Daehwan Chung
    Department of Genetics, University of Georgia, Athens, Georgia, United States of America
    PLoS ONE 7:e43844. 2012
    ....
  3. pmc Metabolic engineering of Caldicellulosiruptor bescii yields increased hydrogen production from lignocellulosic biomass
    Minseok Cha
    Department of Genetics, University of Georgia, Athens, GA 30602, USA
    Biotechnol Biofuels 6:85. 2013
    ..Here, we report the first targeted gene deletion, the gene encoding lactate dehydrogenase (ldh), for metabolic engineering of a member of this genus...
  4. pmc Overcoming restriction as a barrier to DNA transformation in Caldicellulosiruptor species results in efficient marker replacement
    Daehwan Chung
    Department of Genetics, University of Georgia, Athens, GA 30602, USA
    Biotechnol Biofuels 6:82. 2013
    ..Metabolic engineering of pathways for product synthesis is relatively simple compared to engineering the ability to utilize non-pretreated biomass...
  5. pmc An integrative expression vector for Actinosynnema pretiosum
    Shan Goh
    Bioprocessing Technology Institute, 20 Biopolis Way, Centros 06 01, Singapore
    BMC Biotechnol 7:72. 2007
    ..pretiosum is lacking. The aim of this study was to construct a vector for heterologous gene expression in A. pretiosum...
  6. pmc Insights into plant biomass conversion from the genome of the anaerobic thermophilic bacterium Caldicellulosiruptor bescii DSM 6725
    Phuongan Dam
    Institute of Bioinformatics, Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602, USA
    Nucleic Acids Res 39:3240-54. 2011
    ..bescii to convert plant biomass. The high duplication of CAZy domains coupled with the ability to acquire foreign genes by LGT may have allowed the bacterium to rapidly adapt to changing plant biomass-rich environments...
  7. pmc Recombinogenic properties of Pyrococcus furiosus strain COM1 enable rapid selection of targeted mutants
    Joel Farkas
    Department of Genetics, University of Georgia, Athens, USA
    Appl Environ Microbiol 78:4669-76. 2012
    ..The ease and speed of this procedure will facilitate the construction of strains with multiple genetic changes and allow the construction of mutants with deletions of virtually any nonessential gene...
  8. pmc Deletion strains reveal metabolic roles for key elemental sulfur-responsive proteins in Pyrococcus furiosus
    Stephanie L Bridger
    Department of Biochemistry and Molecular Biology, Life Sciences Bldg, University of Georgia, Athens, GA 30602 7229, USA
    J Bacteriol 193:6498-504. 2011
    ..A model is proposed for S(0) reduction by P. furiosus with roles for MBX and NSR in bioenergetics and for SipA in iron-sulfur metabolism...
  9. doi request reprint Classification of 'Anaerocellum thermophilum' strain DSM 6725 as Caldicellulosiruptor bescii sp. nov
    Sung Jae Yang
    Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia 30602, USA
    Int J Syst Evol Microbiol 60:2011-5. 2010
    ..The strain was able to grow up to 90 degrees C (pH 7.2) and degraded crystalline cellulose and xylan as well as untreated plant biomass, including potential bioenergy plants such as poplar and switchgrass...
  10. pmc Defining components of the chromosomal origin of replication of the hyperthermophilic archaeon Pyrococcus furiosus needed for construction of a stable replicating shuttle vector
    Joel Farkas
    Department of Genetics, University of Georgia, Athens, GA 30602, USA
    Appl Environ Microbiol 77:6343-9. 2011
    ..furiosus, plasmids based on the P. furiosus chromosomal origin were structurally unchanged after transformation and were stable without selection for more than 100 generations...
  11. ncbi request reprint Isolation and bioinformatic analysis of a novel transposable element, ISCbe4, from the hyperthermophilic bacterium, Caldicellulosiruptor bescii
    Minseok Cha
    Department of Genetics, University of Georgia, Athens, GA, 30602, USA
    J Ind Microbiol Biotechnol 40:1443-8. 2013
    ....
  12. doi request reprint Improved growth media and culture techniques for genetic analysis and assessment of biomass utilization by Caldicellulosiruptor bescii
    Joel Farkas
    Department of Genetics, University of Georgia, Athens, GA 30602, USA
    J Ind Microbiol Biotechnol 40:41-9. 2013
    ..These new media represent a significant advance for both genetic manipulation and the study of biomass utilization in C. bescii, and may be applied broadly across the Caldicellulosiruptor genus...
  13. pmc Direct conversion of plant biomass to ethanol by engineered Caldicellulosiruptor bescii
    Daehwan Chung
    Department of Genetics, University of Georgia, Athens, GA 30602 andThe BioEnergy Science Center and
    Proc Natl Acad Sci U S A 111:8931-6. 2014
    ..Direct conversion of biomass to ethanol represents a new paradigm for consolidated bioprocessing, offering the potential for carbon neutral, cost-effective, sustainable fuel production. ..
  14. doi request reprint Identification and characterization of CbeI, a novel thermostable restriction enzyme from Caldicellulosiruptor bescii DSM 6725 and a member of a new subfamily of HaeIII-like enzymes
    Dae hwan Chung
    Department of Genetics, University of Georgia, Athens, GA 30602, USA
    J Ind Microbiol Biotechnol 38:1867-77. 2011
    ..A phylogenetic analysis based on sequence alignment and conserved motif searches identified features of CbeI distinct from other members of this group and classified CbeI as a member of a novel subfamily of HaeIII-like enzymes...
  15. pmc Natural competence in the hyperthermophilic archaeon Pyrococcus furiosus facilitates genetic manipulation: construction of markerless deletions of genes encoding the two cytoplasmic hydrogenases
    Gina L Lipscomb
    Department of Genetics, University of Georgia, Athens, GA 30602, USA
    Appl Environ Microbiol 77:2232-8. 2011
    ..The COM1 strain will provide the basis for the development of more sophisticated genetic tools allowing the study and metabolic engineering of this important hyperthermophile...
  16. pmc Efficient degradation of lignocellulosic plant biomass, without pretreatment, by the thermophilic anaerobe "Anaerocellum thermophilum" DSM 6725
    Sung Jae Yang
    Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602, USA
    Appl Environ Microbiol 75:4762-9. 2009
    ..thermophilum DSM 6725, grew well on switchgrass but not on poplar, indicating a significant difference in the biomass-degrading abilities of these two otherwise very similar organisms...
  17. doi request reprint Branched-chain amino acid catabolism provides precursors for the Type II polyketide antibiotic, actinorhodin, via pathways that are nutrient dependent
    Karen Stirrett
    Genetics Department, University of Georgia, Athens, GA, USA
    J Ind Microbiol Biotechnol 36:129-37. 2009
    ....
  18. doi request reprint Detection of a novel active transposable element in Caldicellulosiruptor hydrothermalis and a new search for elements in this genus
    Daehwan Chung
    Department of Genetics, University of Georgia, Athens, GA 30602, USA
    J Ind Microbiol Biotechnol 40:517-21. 2013
    ....
  19. pmc Genome sequence of the anaerobic, thermophilic, and cellulolytic bacterium "Anaerocellum thermophilum" DSM 6725
    Irina A Kataeva
    Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602 7229, USA
    J Bacteriol 191:3760-1. 2009
    ....
  20. pmc A new TetR family transcriptional regulator required for morphogenesis in Streptomyces coelicolor
    Brandan Hillerich
    Genetics Department, University of Georgia, Life Sciences Building, Athens, GA 30602, USA
    J Bacteriol 190:61-7. 2008
    ....