JOHN WEISEL

Summary

Affiliation: University of Pennsylvania
Country: USA

Publications

  1. ncbi request reprint Fibrinogen and fibrin
    John W Weisel
    Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104 6058, USA
    Adv Protein Chem 70:247-99. 2005
  2. pmc Fibrin clots are equilibrium polymers that can be remodeled without proteolytic digestion
    Irina N Chernysh
    Department Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Sci Rep 2:879. 2012
  3. pmc Mechanisms of fibrin polymerization and clinical implications
    John W Weisel
    Department of Cell and Developmental Biology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
    Blood 121:1712-9. 2013
  4. ncbi request reprint The biochemical and physical process of fibrinolysis and effects of clot structure and stability on the lysis rate
    J W Weisel
    Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 6058, USA
    Cardiovasc Hematol Agents Med Chem 6:161-80. 2008
  5. ncbi request reprint Which knobs fit into which holes in fibrin polymerization?
    J W Weisel
    Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    J Thromb Haemost 5:2340-3. 2007
  6. ncbi request reprint Structure of fibrin: impact on clot stability
    J W Weisel
    Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 6058, USA
    J Thromb Haemost 5:116-24. 2007
  7. doi request reprint Biophysics. Enigmas of blood clot elasticity
    John W Weisel
    Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Science 320:456-7. 2008
  8. pmc Multiscale mechanics of fibrin polymer: gel stretching with protein unfolding and loss of water
    Andre E X Brown
    Department of Physics and Astronomy, University of Pennsylvania, Philadelphia, PA 19104, USA
    Science 325:741-4. 2009
  9. ncbi request reprint Fibrinogen Philadelphia, a hypodysfibrinogenemia characterized by abnormal polymerization and fibrinogen hypercatabolism due to gamma S378P mutation
    Margaret A Keller
    Department of Medicine, Jefferson Medical College, Thomas Jefferson University, Philadelphia, PA, USA
    Blood 105:3162-8. 2005
  10. pmc The elasticity of an individual fibrin fiber in a clot
    Jean Philippe Collet
    Department of Cell and Developmental Biology and Institute for Environmental Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Proc Natl Acad Sci U S A 102:9133-7. 2005

Research Grants

Collaborators

Detail Information

Publications65

  1. ncbi request reprint Fibrinogen and fibrin
    John W Weisel
    Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104 6058, USA
    Adv Protein Chem 70:247-99. 2005
    ..Fibrinogen-like domains originated early in evolution, and it is likely that their specific and tightly controlled intermolecular interactions are involved in other aspects of cellular function and developmental biology...
  2. pmc Fibrin clots are equilibrium polymers that can be remodeled without proteolytic digestion
    Irina N Chernysh
    Department Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Sci Rep 2:879. 2012
    ....
  3. pmc Mechanisms of fibrin polymerization and clinical implications
    John W Weisel
    Department of Cell and Developmental Biology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
    Blood 121:1712-9. 2013
    ..There are important clinical consequences to all of these new findings that are relevant for the pathogenesis of diseases, prophylaxis, diagnosis, and treatment...
  4. ncbi request reprint The biochemical and physical process of fibrinolysis and effects of clot structure and stability on the lysis rate
    J W Weisel
    Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 6058, USA
    Cardiovasc Hematol Agents Med Chem 6:161-80. 2008
    ..Platelet aggregation and clot retraction have a dramatic effect on the structure of fibrin and hence on fibrinolysis...
  5. ncbi request reprint Which knobs fit into which holes in fibrin polymerization?
    J W Weisel
    Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    J Thromb Haemost 5:2340-3. 2007
  6. ncbi request reprint Structure of fibrin: impact on clot stability
    J W Weisel
    Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 6058, USA
    J Thromb Haemost 5:116-24. 2007
    ..Basic mechanisms underlying both the mechanical and fibrinolytic characteristics of fibrin are described. Some of the known correlations between clot structure and mechanical and fibrinolytic properties are summarized...
  7. doi request reprint Biophysics. Enigmas of blood clot elasticity
    John W Weisel
    Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Science 320:456-7. 2008
  8. pmc Multiscale mechanics of fibrin polymer: gel stretching with protein unfolding and loss of water
    Andre E X Brown
    Department of Physics and Astronomy, University of Pennsylvania, Philadelphia, PA 19104, USA
    Science 325:741-4. 2009
    ....
  9. ncbi request reprint Fibrinogen Philadelphia, a hypodysfibrinogenemia characterized by abnormal polymerization and fibrinogen hypercatabolism due to gamma S378P mutation
    Margaret A Keller
    Department of Medicine, Jefferson Medical College, Thomas Jefferson University, Philadelphia, PA, USA
    Blood 105:3162-8. 2005
    ..The location, nature, and unusual characteristics of this mutation may add to our understanding of fibrinogen protein interactions necessary for normal catabolism and fibrin formation...
  10. pmc The elasticity of an individual fibrin fiber in a clot
    Jean Philippe Collet
    Department of Cell and Developmental Biology and Institute for Environmental Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Proc Natl Acad Sci U S A 102:9133-7. 2005
    ..Similar values were obtained by other independent methods, including analysis of measurements of fluctuations in bead force as a result of Brownian motion. These results provide a basis for understanding the origin of clot elasticity...
  11. pmc Fibrin gels and their clinical and bioengineering applications
    Paul A Janmey
    Department of Physiology, Institute for Medicine and Engineering, University of Pennsylvania, 3340 Smith Walk, Philadelphia, PA 19104, USA
    J R Soc Interface 6:1-10. 2009
    ..The biochemical and mechanical properties of fibrin have recently been exploited in numerous studies that suggest its potential for applications in medicine and bioengineering...
  12. ncbi request reprint The mechanical properties of fibrin for basic scientists and clinicians
    John W Weisel
    Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, 1054 BRB II III, 421 Curie Boulevard, Philadelphia, PA 19104 6058, USA
    Biophys Chem 112:267-76. 2004
    ..Moreover, these properties are essential to the physiology of blood clotting and are important for understanding and therefore preventing and treating thrombosis...
  13. pmc The molecular structure of human tissue type XV presents a unique conformation among the collagens
    Jeanne C Myers
    Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Biochem J 404:535-44. 2007
    ....
  14. pmc Polymerization of fibrin: specificity, strength, and stability of knob-hole interactions studied at the single-molecule level
    Rustem I Litvinov
    Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, 421 Curie Blvd, 1040 BRB II III, Philadelphia, PA 19104 6058, USA
    Blood 106:2944-51. 2005
    ..3 nm. The results demonstrate that the knob-hole binding during thrombin-induced fibrin polymerization is driven by strong, stable, and highly specific A:a bonding, whereas A:b, B:b, or B:a interactions were not detected...
  15. ncbi request reprint Activation of individual alphaIIbbeta3 integrin molecules by disruption of transmembrane domain interactions in the absence of clustering
    Rustem I Litvinov
    Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
    Biochemistry 45:4957-64. 2006
    ....
  16. pmc Polymerization of fibrin: Direct observation and quantification of individual B:b knob-hole interactions
    Rustem I Litvinov
    Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, 1040 BRB II III, 421 Curie Blvd, Philadelphia, PA 19104 6058, USA
    Blood 109:130-8. 2007
    ..These results directly demonstrate for the first time B:b binding mediated by natural B-knobs exposed in a fibrin monomer...
  17. pmc Fibrin network structure and clot mechanical properties are altered by incorporation of erythrocytes
    Kathryn C Gersh
    Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 6058, USA
    Thromb Haemost 102:1169-75. 2009
    ..RBCs triggered variability in the fibrin network structure, individual fiber characteristics, and overall clot viscoelasticity compared to the absence of cells. These results are important for understanding in vivo clots and thrombi...
  18. pmc Direct evidence for specific interactions of the fibrinogen alphaC-domains with the central E region and with each other
    Rustem I Litvinov
    Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104 6058, USA
    Biochemistry 46:9133-42. 2007
    ..They also confirm the hypothesized homomeric interactions between the alphaC-domains and display their interaction with the alphaC-connectors, which may contribute to covalent cross-linking of alpha polymers in fibrin...
  19. ncbi request reprint Type XIX collagen purified from human umbilical cord is characterized by multiple sharp kinks delineating collagenous subdomains and by intermolecular aggregates via globular, disulfide-linked, and heparin-binding amino termini
    Jeanne C Myers
    Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
    J Biol Chem 278:32047-57. 2003
    ....
  20. ncbi request reprint Activation of integrin alphaIIbbeta3 by modulation of transmembrane helix associations
    Renhao Li
    Department of Biochemistry and Biophysics, School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
    Science 300:795-8. 2003
    ..These results suggest that homomeric associations involving transmembrane domains provide a driving force for integrin activation. They also suggest a structural basis for the coincidence of integrin activation and clustering...
  21. ncbi request reprint Protein-protein unbinding induced by force: single-molecule studies
    John W Weisel
    Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia 19104 6058, USA
    Curr Opin Struct Biol 13:227-35. 2003
    ..Another important aspect of force-induced protein-protein unbinding studies is the new information being gleaned about the thermodynamics and kinetics of bond rupture...
  22. pmc Forced unfolding of coiled-coils in fibrinogen by single-molecule AFM
    Andre E X Brown
    Department of Physics and Astronomy, Nano Bio Interface Center, University of Pennsylvania, Philadelphia, Pennsylvania, USA
    Biophys J 92:L39-41. 2007
    ..The distinct behavior of fibrinogen seems to be due to its topologically complex coiled-coils and an interaction between fibrinogen's alphaC-domains and its central region...
  23. pmc Multi-step fibrinogen binding to the integrin (alpha)IIb(beta)3 detected using force spectroscopy
    Rustem I Litvinov
    Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 6085, USA
    Biophys J 89:2824-34. 2005
    ..The aggregate of data provides evidence for complex multi-step binding/unbinding pathways of alphaIIbbeta3 and fibrinogen revealed at the single-molecule level...
  24. pmc Dissociation of bimolecular αIIbβ3-fibrinogen complex under a constant tensile force
    Rustem I Litvinov
    Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, USA
    Biophys J 100:165-73. 2011
    ..Taken together, these data provide important quantitative and qualitative characteristics of αIIbβ3-fibrinogen binding and unbinding that underlie the dynamics of platelet adhesion and aggregation in blood flow...
  25. pmc Binding strength and activation state of single fibrinogen-integrin pairs on living cells
    Rustem I Litvinov
    Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 6058, USA
    Proc Natl Acad Sci U S A 99:7426-31. 2002
    ..This methodology can be applied to the study of other regulated cell membrane receptors using the ligand-receptor yield strength as a direct measure of receptor activation/inactivation state...
  26. ncbi request reprint Functional characterization of fibrinogen Bicêtre II: a gamma 308 Asn-->Lys mutation located near the fibrin D:D interaction sites
    Rita C Marchi
    Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
    Blood Coagul Fibrinolysis 17:193-201. 2006
    ..This amino acid substitution defines distinctive effects on the surface of the D:D interaction sites that are reflected in the clot structure and functional properties...
  27. pmc Solution structure of the A4 domain of factor XI sheds light on the mechanism of zymogen activation
    Dharmaraj Samuel
    Division of Basic Science, Fox Chase Cancer Center, Philadelphia, PA 19111, USA
    Proc Natl Acad Sci U S A 104:15693-8. 2007
    ..The results are consistent with biochemical evidence that activated FXI cleaves its substrate at two positions without release of an intermediate...
  28. pmc Fibrinogen beta-chain tyrosine nitration is a prothrombotic risk factor
    Ioannis Parastatidis
    Stokes Research Institute and Departments of Pediatrics and Pharmacology, Children s Hospital of Philadelphia and The University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    J Biol Chem 283:33846-53. 2008
    ..The data provide a novel biochemical risk factor that could explain epidemiological associations of oxidative stress and inflammation with thrombotic complications...
  29. ncbi request reprint Activation of platelet alphaIIbbeta3 by an exogenous peptide corresponding to the transmembrane domain of alphaIIb
    Hang Yin
    Department of Biochemistry and Biophysics, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    J Biol Chem 281:36732-41. 2006
    ..These observations demonstrate that transmembrane domain interactions regulate integrin function in situ and that it is possible to target intra-membranous protein-protein interactions in a way that can have functional consequences...
  30. pmc Complexation of peptidoglycan intermediates by the lipoglycodepsipeptide antibiotic ramoplanin: minimal structural requirements for intermolecular complexation and fibril formation
    Predrag Cudic
    Johnson Research Foundation and the Department of Biochemistry, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 6059, USA
    Proc Natl Acad Sci U S A 99:7384-9. 2002
    ....
  31. ncbi request reprint Pro-thrombotic state induced by post-translational modification of fibrinogen by reactive nitrogen species
    Caryn Vadseth
    Stokes Research Institute and Department of Biochemistry and Biophysics, Children s Hospital of Philadelphia, Philadelphia, PA 19104, USA
    J Biol Chem 279:8820-6. 2004
    ..The present results may account, in part, for the association between nitrative stress and risk for coronary artery disease...
  32. ncbi request reprint Functional and structural correlations of individual alphaIIbbeta3 molecules
    Rustem I Litvinov
    Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, 421 Curie Blvd, 1054 BRB II III, Philadelphia, PA 19104 6058, USA
    Blood 104:3979-85. 2004
    ..Further, they are consistent with the presence of a linked equilibrium between single inactive and single active alphaIIbbeta3 molecules and active alphaIIbbeta3 clusters...
  33. pmc Dynamic imaging of fibrin network formation correlated with other measures of polymerization
    Irina N Chernysh
    Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, USA
    Blood 111:4854-61. 2008
    ..These results provide new information on the relative time sequence of events during fibrin network formation...
  34. ncbi request reprint Quantitative analysis of platelet alpha v beta 3 binding to osteopontin using laser tweezers
    Rustem I Litvinov
    Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, 19104 6058, USA
    J Biol Chem 278:51285-90. 2003
    ..Furthermore, they indicate that platelet adhesion to osteopontin-coated surfaces requires an agonist-induced exposure of alpha v beta 3-binding sites for this ligand...
  35. ncbi request reprint A novel mutation (deletion of Aalpha-Asn 80) in an abnormal fibrinogen: fibrinogen Caracas VI. Consequences of disruption of the coiled coil for the polymerization of fibrin: peculiar clot structure and diminished stiffness of the clot
    Rita C Marchi
    Department of Cell and Developmental Biology, University of Pennsylvania, Philadelphia, USA
    Blood Coagul Fibrinolysis 15:559-67. 2004
    ..This abnormal fibrinogen has been named fibrinogen Caracas VI...
  36. ncbi request reprint Functional analysis of the lipoglycodepsipeptide antibiotic ramoplanin
    Predrag Cudic
    Johnson Research Foundation, Department of Biochemistry and Biophysics, Philadelphia, PA 19104, USA
    Chem Biol 9:897-906. 2002
    ..These results help define a minimalist ramoplanin pharmacophore and introduce the possibility of generating ramoplanin-derived peptide or peptidomimetic antibiotics for use against VRE, MRSA, and related pathogens...
  37. ncbi request reprint Dynamic regulation of microtubule coils in ADP-induced platelet shape change by p160ROCK (Rho-kinase)
    Benjamin Z S Paul
    Department of Pharmacology, Temple University School of Medicine, Philadelphia, PA 19140, USA
    Platelets 14:159-69. 2003
    ..Continued disruption of the cytoskeletal microtubule ring, appears to be a Rhokinase-dependent event involved in the transformation of discoid platelets into spheres...
  38. ncbi request reprint Homophenotypic Aalpha R16H fibrinogen (Kingsport): uniquely altered polymerization associated with slower fibrinopeptide A than fibrinopeptide B release
    Dennis K Galanakis
    Blood Bank, L5, University Hospital, HSC, SUNY, Stony Brook, New York 11794 7530, USA
    Blood Coagul Fibrinolysis 18:731-7. 2007
    ..We hypothesize that during normal polymerization these constitutive des-BB fibrin monomer properties attenuate their des-AA fibrin counterparts...
  39. pmc Glycaemic control improves fibrin network characteristics in type 2 diabetes - a purified fibrinogen model
    Marlien Pieters
    School of Physiology, Nutrition and Consumer Science, Department Nutrition, North West University, Potchefstroom Campus, Private Bag X6001, Potchefstroom 2520, South Africa
    Thromb Haemost 99:691-700. 2008
    ..Achievement of glycaemic control and decreased fibrinogen glycation level improves permeability and lysis rates in a purified fibrinogen model...
  40. ncbi request reprint Incorporation of fibrin molecules containing fibrinopeptide A alters clot ultrastructure and decreases permeability
    Veronica H Flood
    Division of Pediatric Hematology Oncology, School of Medicine, Oregon Health and Science University, Portland, OR 97239 3098, USA
    Br J Haematol 138:117-24. 2007
    ....
  41. ncbi request reprint Functional analysis of fibrin {gamma}-chain cross-linking by activated factor XIII: determination of a cross-linking pattern that maximizes clot stiffness
    Kristina F Standeven
    Academic Unit of Molecular Vascular Medicine, Leeds Institute for Genetics, Health and Therapeutics, Clarendon Way, University of Leeds, UK
    Blood 110:902-7. 2007
    ..Our results show that gamma-chain cross-linking contributes significantly to clot stiffness, in particular through gamma-dimer formation; alpha-gamma hybrid cross-links had the smallest impact on clot stiffness...
  42. pmc Comparison of the effect of different homocysteine concentrations on clot formation using human plasma and purified fibrinogen
    Rita Marchi
    Thromb Haemost 99:451-2. 2008
  43. ncbi request reprint Common variation in the C-terminal region of the fibrinogen beta-chain: effects on fibrin structure, fibrinolysis and clot rigidity
    Ramzi Ajjan
    Academic Unit of Molecular Vascular Medicine, Leeds Institute for Genetics, Health and Therapeutics, University of Leeds, UK
    Blood 111:643-50. 2008
    ..Fibrinogen BbetaArg448Lys is a primary example of common genetic variation with a significant phenotypic effect at the molecular level...
  44. ncbi request reprint Biophysical characterization of fibrinogen Caracas I with an Aalpha-chain truncation at Aalpha-466 Ser: identification of the mutation and biophysical characterization of properties of clots from plasma and purified fibrinogen
    Rita Marchi
    Centro de Medicina Experimental, Instituto Venezolano de Investigaciones Cientificas IVIC, Caracas, República Bolivariana de Venezuela
    Blood Coagul Fibrinolysis 15:285-93. 2004
    ....
  45. ncbi request reprint Recombinant BbetaArg14His fibrinogen implies participation of N-terminus of Bbeta chain in desA fibrin polymerization
    Jennifer L Moen
    Department of Chemistry, University of North Carolina at Chapel Hill, USA
    Blood 102:2466-71. 2003
    ..We conclude that the N-terminus of the Bbeta chain is involved in the lateral aggregation of normal desAprotofibrils and that the Arg-->His substitution disrupts these interactions in BbetaArg14His fibrinogen...
  46. ncbi request reprint Effect of freeze-drying, freezing and frozen storage of blood plasma on fibrin network characteristics
    Marlien Pieters
    Potchefstroom Institute of Nutrition, Potchefstroom University for Christian Higher Education, Private Bag X6001, Potchefstroom 2520, South Africa
    Thromb Res 107:263-9. 2002
    ..We investigated the effect of freezing, freeze-drying and the duration of frozen storage of blood plasma on fibrin network characteristics of clots subsequently produced...
  47. ncbi request reprint The effect of dimethylbiguanide on thrombin activity, FXIII activation, fibrin polymerization, and fibrin clot formation
    Kristina F Standeven
    Academic Unit of Molecular Vascular Medicine, University of Leeds, Leeds, UK
    Diabetes 51:189-97. 2002
    ..These findings indicate that part of the cardioprotective effect of DMB in patients with type 2 diabetes may be attributed to alterations in fibrin structure/function...
  48. ncbi request reprint Ligand binding promotes the entropy-driven oligomerization of integrin alpha IIb beta 3
    Roy R Hantgan
    Department of Biochemistry, Wake Forest University School of Medicine, Winston Salem, North Carolina 27157, USA
    J Biol Chem 278:3417-26. 2003
    ..We propose that entropy-favorable nonpolar interactions drive ligand-induced integrin clustering and outside-in signaling...
  49. ncbi request reprint Ultralarge complexes of PF4 and heparin are central to the pathogenesis of heparin-induced thrombocytopenia
    Lubica Rauova
    Division of Hematology, Children s Hospital of Philadelphia, PA, USA
    Blood 105:131-8. 2005
    ....
  50. ncbi request reprint The disintegrin echistatin stabilizes integrin alphaIIbbeta3's open conformation and promotes its oligomerization
    Roy R Hantgan
    Department of Biochemistry, Wake Forest University School of Medicine, Winston Salem, NC 27517, USA
    J Mol Biol 342:1625-36. 2004
    ....
  51. ncbi request reprint Do the isolated fibrinogen alphaC-domains form ordered oligomers?
    Galina Tsurupa
    Biochemistry Department, Jerome H Holland Laboratory for the Biomedical Sciences, American Red Cross, 15601 Crabbs Branch Way, Rockville, MD 20855, USA
    Biophys Chem 112:257-66. 2004
    ..These results demonstrate that the recombinant alphaC-domains can form stable oligomers which may mimic properties of the alphaC-domains in cross-linked fibrin...
  52. pmc Transglutaminase-mediated oligomerization of the fibrin(ogen) alphaC domains promotes integrin-dependent cell adhesion and signaling
    Alexey M Belkin
    University of Maryland School of Medicine, 15601 Crabbs Branch Way, Rockville, MD 20855, USA
    Blood 105:3561-8. 2005
    ..They also suggest a new role for transglutaminases in regulation of integrin-mediated adhesion and signaling via covalent modification of integrin ligands...
  53. pmc The alphaC domains of fibrinogen affect the structure of the fibrin clot, its physical properties, and its susceptibility to fibrinolysis
    Jean Philippe Collet
    Institut de Cardiologie, Hopital Pitie Salpetriere, Assistance Publique Hopitaux de Paris, France
    Blood 106:3824-30. 2005
    ..In conclusion, these studies provide the first definitive evidence that the alphaC domains play an important role in determining the structure and biophysical properties of clots and their susceptibility to fibrinolysis...
  54. ncbi request reprint Dynamic changes of fibrin architecture during fibrin formation and intrinsic fibrinolysis of fibrin-rich clots
    Jean Philippe Collet
    Institut de Cardiologie, Centre Hospitalier Universitaire Pitié Salpêtrière AP HP, 47 Boulevard de l Hopital, 75013 Paris, France
    J Biol Chem 278:21331-5. 2003
    ....
  55. ncbi request reprint Role of factor XIII in fibrin clot formation and effects of genetic polymorphisms
    Robert A S Ariens
    Academic Unit of Molecular Vascular Medicine, University of Leeds School of Medicine, United Kingdom
    Blood 100:743-54. 2002
    ..In this review, we have summarized recent findings on the structure and function of factor XIII. This is followed by a review of the effects of genetic polymorphisms on protein structure/function and their relationship to disease...
  56. ncbi request reprint The effect of glycaemic control on fibrin network structure of type 2 diabetic subjects
    Marlien Pieters
    Department of Nutrition, North West University, Potchefstroom Campus, Private Bag X6001, Potchefstroom, 2520 South Africa
    Thromb Haemost 96:623-9. 2006
    ..It seems as though porosity, compaction and kinetics of clot formation are more related to fibrinogen concentration than fibrinogen glycation in this model...
  57. ncbi request reprint Interactions mediated by the N-terminus of fibrinogen's Bbeta chain
    Oleg V Gorkun
    Department of Pathology and Laboratory Medicine, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599 7525, USA
    Biochemistry 45:14843-52. 2006
    ..The data demonstrate the existence of weak transient interactions within and between fibrin molecules mediated by the N-terminus of the fibrinogen Bbeta chain...
  58. ncbi request reprint Tirofiban blocks platelet adhesion to fibrin with minimal perturbation of GpIIb/IIIa structure
    Roy R Hantgan
    Department of Biochemistry, Wake Forest University School of Medicine, Winston Salem, NC 27157 1019, USA
    Thromb Haemost 87:910-7. 2002
    ..Our results provide new insights into the mechanisms and potential prothrombotic complications of integrin antagonists...
  59. ncbi request reprint Functional analysis of the fibrinogen Aalpha Thr312Ala polymorphism: effects on fibrin structure and function
    Kristina F Standeven
    Academic Unit of Molecular Vascular Medicine, Research School of Medicine, University of Leeds, Leeds General Infirmary, Leeds, UK
    Circulation 107:2326-30. 2003
    ..We have previously shown an association of Ala312 fibrinogen with poststroke mortality in subjects with atrial fibrillation and with pulmonary embolism in subjects with venous thrombosis...
  60. ncbi request reprint Genetic regulation of fibrin structure and function: complex gene-environment interactions may modulate vascular risk
    Bernard C B Lim
    Academic Unit of Molecular Vascular Medicine, University of Leeds, Leeds, UK
    Lancet 361:1424-31. 2003
    ..We aimed to investigate interactions between polymorphisms in the factor XIII and fibrinogen genes, fibrinogen concentrations, and other cardiovascular risk factors in relation to fibrin structure and function...
  61. ncbi request reprint Fibrinogen Guarenas, an abnormal fibrinogen with an Aalpha-chain truncation due to a nonsense mutation at Aalpha 467 Glu (GAA)-->stop (TAA)
    Rita Marchi
    Centro de Medicina Experimental, Instituto Venezolano de Investigaciones Cientificas, Laboratorio de Hemostasia IVIC, Apartado 21827, Caracas, República Bolivariana de Venezuela
    Thromb Res 118:637-50. 2006
    ..It seems that the increased clot rigidity, decreased porosity, hypofibrinolysis and t-PA induced fibrinolysis, by itself are not necessarily associated with thrombotic disorders in dysfibrinogenemia...
  62. ncbi request reprint Haemostatic changes related to fibrin formation and fibrinolysis during the first trimester in normal pregnancy and in recurrent miscarriage
    Rita Marchi
    Centro de Medicina Experimental, Laboratorio Biología del Desarollo de la Hemostasia, Instituto Venezolano de Investigaciones Cientificas, IVIC Apartado 21827, Caracas, Venezuela
    Thromb Haemost 97:552-7. 2007
    ..In this study we have found changes in the clot structure that seem to be related to normal pregnancy and an increased rate of the fibrin lysis process in the RM patients, which may have clinical relevance...
  63. ncbi request reprint Images in cardiovascular medicine. Architecture of intracoronary thrombi in ST-elevation acute myocardial infarction: time makes the difference
    Farzin Beygui
    Department of Cardiology, Pitie Salpetriere University Hospital, Paris, France
    Circulation 113:e21-3. 2006
  64. ncbi request reprint Glycation of fibrinogen in uncontrolled diabetic patients and the effects of glycaemic control on fibrinogen glycation
    Marlien Pieters
    School of Physiology, Nutrition and Consumer Science, Department of Nutrition, North West University, Potchefstroom Campus, Potchefstroom, South Africa
    Thromb Res 120:439-46. 2007
    ....
  65. pmc Integrin conformational regulation: uncoupling extension/tail separation from changes in the head region by a multiresolution approach
    Mattia Rocco
    S S Biopolimeri e Proteomica, Istituto Nazionale per la Ricerca sul Cancro, IST c o CBA, Genoa, Italy
    Structure 16:954-64. 2008
    ..Extension and immediate tail separation are then uncoupled from head region rearrangements following activation, thus underscoring integrins' delicate, finely tuned plasticity...

Research Grants25

  1. STRUCTURAL STUDIES OF BLOOD CLOTTING PROTEINS
    JOHN WEISEL; Fiscal Year: 2000
    ....
  2. STRUCTURAL STUDIES OF BLOOD CLOTTING PROTEINS
    JOHN WEISEL; Fiscal Year: 2001
    ..The results of these studies will help us to understand molecular mechanisms of fibrinolysis, which may have clinical implications for the treatment and prevention of thrombotic disorders. ..
  3. STRUCTURAL STUDIES OF BLOOD CLOTTING PROTEINS
    JOHN WEISEL; Fiscal Year: 2002
    ..The results of these studies will help us to understand molecular mechanisms of fibrinolysis, which may have clinical implications for the treatment and prevention of thrombotic disorders. ..
  4. STRUCTURAL STUDIES OF BLOOD CLOTTING PROTEINS
    JOHN WEISEL; Fiscal Year: 2003
    ..The results of these studies will help us to understand molecular mechanisms of fibrinolysis, which may have clinical implications for the treatment and prevention of thrombotic disorders. ..
  5. STRUCTURAL STUDIES OF BLOOD CLOTTING PROTEINS
    JOHN WEISEL; Fiscal Year: 2004
    ..The results of these studies will help us to understand molecular mechanisms of fibrinolysis, which may have clinical implications for the treatment and prevention of thrombotic disorders. ..
  6. STRUCTURAL STUDIES OF BLOOD CLOTTING PROTEINS
    JOHN WEISEL; Fiscal Year: 2005
    ..abstract_text> ..
  7. STRUCTURAL STUDIES OF BLOOD CLOTTING PROTEINS
    JOHN WEISEL; Fiscal Year: 2006
    ..abstract_text> ..
  8. STRUCTURAL STUDIES OF BLOOD CLOTTING PROTEINS
    JOHN WEISEL; Fiscal Year: 2007
    ..abstract_text> ..
  9. Structural origin of fibrin clot mechanical properties
    JOHN WEISEL; Fiscal Year: 2009
    ..More generally, this research involves the determination of relationships between molecular structure and the mechanical properties of a remarkable biological material, the blood clot. ..
  10. STRUCTURAL STUDIES OF BLOOD CLOTTING PROTEINS
    JOHN WEISEL; Fiscal Year: 1999
    ....
  11. STRUCTURAL STUDIES OF BLOOD CLOTTING PROTEINS
    JOHN WEISEL; Fiscal Year: 1992
    ..to develop more effective and specific methods to control these processes, it is necessary to understand the molecular structures and interactions of the proteins involved...
  12. STRUCTURAL STUDIES OF BLOOD CLOTTING PROTEINS
    JOHN WEISEL; Fiscal Year: 1993
    ....
  13. STRUCTURAL STUDIES OF BLOOD CLOTTING PROTEINS
    JOHN WEISEL; Fiscal Year: 1990
    ..to develop more effective and specific methods to control these processes, it is necessary to understand the molecular structures and interactions of the proteins involved...
  14. STRUCTURAL STUDIES OF BLOOD CLOTTING PROTEINS
    JOHN WEISEL; Fiscal Year: 1991
    ..to develop more effective and specific methods to control these processes, it is necessary to understand the molecular structures and interactions of the proteins involved...
  15. Structural origin of fibrin clot mechanical properties
    John W Weisel; Fiscal Year: 2010
    ..More generally, this research involves the determination of relationships between molecular structure and the mechanical properties of a remarkable biological material, the blood clot. ..