C M Waterman-Storer
Affiliation: University of North Carolina
- Fluorescent speckle microscopy, a method to visualize the dynamics of protein assemblies in living cellsC M Waterman-Storer
Department of Biology, University of North Carolina, Chapel Hill 27599 3280, USA
Curr Biol 8:1227-30. 1998..Our initial applications include the measurement of microtubule movements in mitotic spindles and actin retrograde flow in migrating cells...
- Microtubule growth activates Rac1 to promote lamellipodial protrusion in fibroblastsC M Waterman-Storer
Department of Biology, University of North Carolina, Chapel Hill 27599, USA
Nat Cell Biol 1:45-50. 1999..We suggest that the growth phase of microtubule dynamic instability at leading-edge lamellipodia locally activates Rac1 to drive actin polymerization and lamellipodial protrusion required for cell migration...
- Microtubule dynamics: treadmilling comes around againC M Waterman-Storer
Department of Biology, CB 3280, 607 Fordham Hall, University of North Carolina, Chapel Hill, North Carolina 27599, USA
Curr Biol 7:R369-72. 1997....
- Actomyosin-based retrograde flow of microtubules in the lamella of migrating epithelial cells influences microtubule dynamic instability and turnover and is associated with microtubule breakage and treadmillingC M Waterman-Storer
Department of Biology, 607 Fordham Hall, University of North Carolina, Chapel Hill, North Carolina 27599 3280, USA
J Cell Biol 139:417-34. 1997..We propose that actomyosin-based retrograde flow of MTs causes MT breakage, forming quasi-stable noncentrosomal MTs whose turnover is regulated primarily at their minus ends...
- The interaction between cytoplasmic dynein and dynactin is required for fast axonal transportC M Waterman-Storer
Department of Animal Biology, University of Pennsylvania, Philadelphia, PA 19104, USA
Proc Natl Acad Sci U S A 94:12180-5. 1997....
- How microtubules get fluorescent specklesC M Waterman-Storer
Department of Biology, University of North Carolina, Chapel Hill, North Carolina 27599, USA
Biophys J 75:2059-69. 1998..Speckle patterns can provide fiduciary marks in the microtubule lattice for motility studies or can be used to determine the fraction of labeled tubulin microinjected into living cells...
- Positive feedback interactions between microtubule and actin dynamics during cell motilityC M Waterman-Storer
Department of Biology 607 Fordham Hall University of North Carolina Chapel Hill NC 27599 3280 USA
Curr Opin Cell Biol 11:61-7. 1999..Thus, an actin-dependent gradient in microtubule dynamic-instability parameters in cells may feed back through the activation of specific signalling pathways to perpetuate the polarized actin-assembly dynamics required for cell motility...
- Microtubule/organelle motility assaysC M Waterman-Storer
University of North Carolina, Chapel Hill, North Carolina, USA
Curr Protoc Cell Biol . 2001....
- The product of the Drosophila gene, Glued, is the functional homologue of the p150Glued component of the vertebrate dynactin complexC M Waterman-Storer
Department of Animal Biology, University of Pennsylvania School of Veterinary Medicine, Philadelphia 19104 6046, USA
J Biol Chem 271:1153-9. 1996....
- Rapid dynamics of the microtubule binding of ensconsin in vivoJ C Bulinski
Department of Biological Sciences, Columbia University, College of Physicians and Surgeons, 630 W 168th St, Rm BB1213, New York, NY 10032 3702, USA
J Cell Sci 114:3885-97. 2001..Our results show that ensconsin is highly dynamic in its association with microtubules, and its microtubule association can be altered by in vivo phosphorylation events...
- The p150Glued component of the dynactin complex binds to both microtubules and the actin-related protein centractin (Arp-1)C M Waterman-Storer
Department of Animal Biology, University of Pennsylvania School of Veterinary Medicine, Philadelphia 19104 6046
Proc Natl Acad Sci U S A 92:1634-8. 1995..These results demonstrate that p150Glued, a protein implicated in cytoplasmic dynein-based microtubule motility, is capable of direct binding to both microtubules and centractin...
- Cell motility: can Rho GTPases and microtubules point the way?T Wittmann
The Scripps Research Institute, Department of Cell Biology, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
J Cell Sci 114:3795-803. 2001..Thus, microtubules might be part of a positive feedback mechanism that maintains the stable polarization of a directionally migrating cell...
- Recovery, visualization, and analysis of actin and tubulin polymer flow in live cells: a fluorescent speckle microscopy studyP Vallotton
BioMicroMetrics Group, Laboratory for Biomechanics, ETH Zurich, 8952 Schlieren, Switzerland
Biophys J 85:1289-306. 2003..Third, we analyze microtubule poleward flux in mitotic metaphase spindles assembled in Xenopus egg extracts, bringing new insight into the dynamics of microtubule assemblies in this system...