Research Topics
Species | Zefeng WangSummaryAffiliation: University of North Carolina Country: USA Publications
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Detail Information
Publications
Splicing regulation: from a parts list of regulatory elements to an integrated splicing codeZefeng Wang
Department of Pharmacology, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
RNA 14:802-13. 2008..Here, we summarize the current state of knowledge of splicing cis-regulatory elements and their context-dependent effects on splicing, emphasizing recent global/genome-wide studies and open questions...
A complex network of factors with overlapping affinities represses splicing through intronic elementsYang Wang
Department of Pharmacology, Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, North Carolina, USA
Nat Struct Mol Biol 20:36-45. 2013..This arrangement enables individual cis elements to function differently in distinct cellular contexts, depending on the spectrum of regulatory factors present...
Engineering splicing factors with designed specificitiesYang Wang
Department of Pharmacology, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA
Nat Methods 6:825-30. 2009..Our approach permitted the creation of artificial factors to target virtually any pre-mRNA, providing a strategy to study splicing regulation and to manipulate disease-associated splicing events...
Quantitative analysis of focused a-to-I RNA editing sites by ultra-high-throughput sequencing in psychiatric disordersHu Zhu
Department of Pharmacology, University of North Carolina Chapel Hill Medical School, Chapel Hill, North Carolina, United States of America
PLoS ONE 7:e43227. 2012....
Specific and modular binding code for cytosine recognition in Pumilio/FBF (PUF) RNA-binding domainsShuyun Dong
Department of Pharmacology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
J Biol Chem 286:26732-42. 2011..This work deepens our understanding of natural PUF protein target recognition and expands the ability to engineer PUF domains to recognize any RNA sequence...
Engineering RNA endonucleases with customized sequence specificitiesRajarshi Choudhury
Department of Pharmacology and Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
Nat Commun 3:1147. 2012....
Intronic splicing enhancers, cognate splicing factors and context-dependent regulation rulesYang Wang
Department of Pharmacology, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA
Nat Struct Mol Biol 19:1044-52. 2012..We provide a comprehensive picture of general ISE activities and suggest new models of how single elements can function oppositely, depending on locations and binding factors...
Assessing serotonin receptor mRNA editing frequency by a novel ultra high-throughput sequencing methodAtheir I Abbas
Department of Biochemistry, School of Medicine, Case Western Reserve University, Cleveland, OH 44106, USA
Nucleic Acids Res 38:e118. 2010....
Coevolutionary networks of splicing cis-regulatory elementsXinshu Xiao
Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
Proc Natl Acad Sci U S A 104:18583-8. 2007..Thus, the splicing elements defining exons coevolve in a way that preserves overall exon strength, allowing specific elements to substitute for loss or weakening of others...
General and specific functions of exonic splicing silencers in splicing controlZefeng Wang
Department of Biology, Massachusetts Institute of Technology, Cambridge, 02139, USA
Mol Cell 23:61-70. 2006..Together, our findings provide a comprehensive picture of the functions of ESSs in the control of diverse types of splicing decisions...
Systematic identification and analysis of exonic splicing silencersZefeng Wang
Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
Cell 119:831-45. 2004..ExonScan and related bioinformatic analyses suggest that these ESS motifs play important roles in suppression of pseudoexons, in splice site definition, and in AS...
Glycolysis modulates trypanosome glycoprotein expression as revealed by an RNAi libraryJames C Morris
Department of Biological Chemistry, Johns Hopkins Medical School, 725 N. Wolfe Street, Baltimore, MD 21205, USA
EMBO J 21:4429-38. 2002..These data suggest that T.brucei 'senses' changes in glucose level and modulates procyclin expression accordingly...
