Yinsheng Wang

Summary

Affiliation: University of California
Country: USA

Publications

  1. pmc Quantification of oxidative single-base and intrastrand cross-link lesions in unmethylated and CpG-methylated DNA induced by Fenton-type reagents
    Huachuan Cao
    Department of Chemistry 027, University of California, Riverside, CA 92521 0403, USA
    Nucleic Acids Res 35:4833-44. 2007
  2. pmc High-throughput analysis of the mutagenic and cytotoxic properties of DNA lesions by next-generation sequencing
    Bifeng Yuan
    Department of Chemistry, University of California, Riverside, CA 92521 0403, USA
    Nucleic Acids Res 39:5945-54. 2011
  3. pmc Generation of 5-(2'-deoxycytidyl)methyl radical and the formation of intrastrand cross-link lesions in oligodeoxyribonucleotides
    Qibin Zhang
    Department of Chemistry, University of California at Riverside Riverside, CA 92521 0403, USA
    Nucleic Acids Res 33:1593-603. 2005
  4. pmc A quantitative assay for assessing the effects of DNA lesions on transcription
    Changjun You
    Department of Chemistry, University of California Riverside, Riverside, CA, USA
    Nat Chem Biol 8:817-22. 2012
  5. pmc Effects of Tet-induced oxidation products of 5-methylcytosine on Dnmt1- and DNMT3a-mediated cytosine methylation
    Debin Ji
    Department of Chemistry, University of California, Riverside, CA 92521, USA
    Mol Biosyst 10:1749-52. 2014
  6. pmc Synthesis and characterization of oligodeoxyribonucleotides containing a site-specifically incorporated N6-carboxymethyl-2'-deoxyadenosine or N4-carboxymethyl-2'-deoxycytidine
    Jianshuang Wang
    Department of Chemistry, University of California, Riverside, CA 92521 0403, USA
    Nucleic Acids Res 38:6774-84. 2010
  7. pmc Kinetics of deamination and Cu(II)/H2O2/Ascorbate-induced formation of 5-methylcytosine glycol at CpG sites in duplex DNA
    Huachuan Cao
    Department of Chemistry and Environmental Toxicology Graduate Program, University of California, Riverside, CA 92521 0403, USA
    Nucleic Acids Res 37:6635-43. 2009
  8. pmc Sequence-dependent formation of intrastrand crosslink products from the UVB irradiation of duplex DNA containing a 5-bromo-2'-deoxyuridine or 5-bromo-2'-deoxycytidine
    Yu Zeng
    Department of Chemistry 027, University of California, Riverside, CA 92521 0403, USA
    Nucleic Acids Res 34:6521-9. 2006
  9. pmc Formation and genotoxicity of a guanine-cytosine intrastrand cross-link lesion in vivo
    Haizheng Hong
    Environmental Toxicology Graduate Program, University of California, Riverside, CA 92521 0403, USA
    Nucleic Acids Res 35:7118-27. 2007
  10. pmc Chemical synthesis of oligodeoxyribonucleotides containing N3- and O4-carboxymethylthymidine and their formation in DNA
    Jianshuang Wang
    Department of Chemistry, University of California, Riverside, CA 92521 0403, USA
    Nucleic Acids Res 37:336-45. 2009

Research Grants

Collaborators

Detail Information

Publications95

  1. pmc Quantification of oxidative single-base and intrastrand cross-link lesions in unmethylated and CpG-methylated DNA induced by Fenton-type reagents
    Huachuan Cao
    Department of Chemistry 027, University of California, Riverside, CA 92521 0403, USA
    Nucleic Acids Res 35:4833-44. 2007
    ..These results suggest that oxidative intrastrand cross-link lesions formed at methylated-CpG sites may account for the previously reported mCG-->TT tandem double mutations induced by Fenton-type reagents...
  2. pmc High-throughput analysis of the mutagenic and cytotoxic properties of DNA lesions by next-generation sequencing
    Bifeng Yuan
    Department of Chemistry, University of California, Riverside, CA 92521 0403, USA
    Nucleic Acids Res 39:5945-54. 2011
    ..Taken together, the newly developed method provided a high-throughput and readily affordable method for assessing quantitatively how DNA lesions compromise the efficiency and fidelity of DNA replication in cells...
  3. pmc Generation of 5-(2'-deoxycytidyl)methyl radical and the formation of intrastrand cross-link lesions in oligodeoxyribonucleotides
    Qibin Zhang
    Department of Chemistry, University of California at Riverside Riverside, CA 92521 0403, USA
    Nucleic Acids Res 33:1593-603. 2005
    ....
  4. pmc A quantitative assay for assessing the effects of DNA lesions on transcription
    Changjun You
    Department of Chemistry, University of California Riverside, Riverside, CA, USA
    Nat Chem Biol 8:817-22. 2012
    ..This newly developed CTAB assay should be generally applicable for quantitatively assessing how other DNA lesions affect DNA transcription in vitro and in cells...
  5. pmc Effects of Tet-induced oxidation products of 5-methylcytosine on Dnmt1- and DNMT3a-mediated cytosine methylation
    Debin Ji
    Department of Chemistry, University of California, Riverside, CA 92521, USA
    Mol Biosyst 10:1749-52. 2014
    ..Our results provided important new knowledge about the implications of the 5-methylcytosine oxidation products in maintenance cytosine methylation. ..
  6. pmc Synthesis and characterization of oligodeoxyribonucleotides containing a site-specifically incorporated N6-carboxymethyl-2'-deoxyadenosine or N4-carboxymethyl-2'-deoxycytidine
    Jianshuang Wang
    Department of Chemistry, University of California, Riverside, CA 92521 0403, USA
    Nucleic Acids Res 38:6774-84. 2010
    ..The formation and the mutagenic potential of N(6)-CMdA and N(4)-CMdC suggest that these lesions may bear important implications in the etiology of NOC-induced tumor development...
  7. pmc Kinetics of deamination and Cu(II)/H2O2/Ascorbate-induced formation of 5-methylcytosine glycol at CpG sites in duplex DNA
    Huachuan Cao
    Department of Chemistry and Environmental Toxicology Graduate Program, University of California, Riverside, CA 92521 0403, USA
    Nucleic Acids Res 37:6635-43. 2009
    ..Together, our data support that the formation and deamination of 5-methylcytosine glycol may contribute significantly to the C -->T transition mutation at mCpG dinucleotide site...
  8. pmc Sequence-dependent formation of intrastrand crosslink products from the UVB irradiation of duplex DNA containing a 5-bromo-2'-deoxyuridine or 5-bromo-2'-deoxycytidine
    Yu Zeng
    Department of Chemistry 027, University of California, Riverside, CA 92521 0403, USA
    Nucleic Acids Res 34:6521-9. 2006
    ..The formation of intrastrand crosslink products from these halopyrimidines in duplex DNA may account for the photosensitizing effects of these nucleosides...
  9. pmc Formation and genotoxicity of a guanine-cytosine intrastrand cross-link lesion in vivo
    Haizheng Hong
    Environmental Toxicology Graduate Program, University of California, Riverside, CA 92521 0403, USA
    Nucleic Acids Res 35:7118-27. 2007
    ..7% G-->T and a 1.2% G-->C transversion mutations. DNA replication in E. coli hosts deficient in SOS-induced polymerases revealed that polymerase V was responsible for the error-prone translesion synthesis in vivo...
  10. pmc Chemical synthesis of oligodeoxyribonucleotides containing N3- and O4-carboxymethylthymidine and their formation in DNA
    Jianshuang Wang
    Department of Chemistry, University of California, Riverside, CA 92521 0403, USA
    Nucleic Acids Res 37:336-45. 2009
    ....
  11. pmc In vitro replication and thermodynamic studies of methylation and oxidation modifications of 6-thioguanine
    Chunang Gu
    Environmental Toxicology Graduate Program, University of California, Riverside, CA 92521 0403, USA
    Nucleic Acids Res 35:3693-704. 2007
    ..Taken together, the results from this study shed important new light on the biological implications of the two metabolites of 6-TG...
  12. pmc Endogenous formation and repair of oxidatively induced G[8-5 m]T intrastrand cross-link lesion
    Jin Wang
    Department of Chemistry, University of California, Riverside, CA 92521 0403, USA
    Nucleic Acids Res 40:7368-74. 2012
    ..Together, our studies established that d(G[8-5 m]T) can be induced endogenously in mammalian tissues and constitutes a substrate for NER in vivo...
  13. pmc Detection of oxidation products of 5-methyl-2'-deoxycytidine in Arabidopsis DNA
    Shuo Liu
    Environmental Toxicology Graduate Program, University of California Riverside, Riverside, California, United States of America
    PLoS ONE 8:e84620. 2013
    ..The relatively low levels of the 5-mdC oxidation products suggest that they arise likely from reactive oxygen species present in cells, which is in line with the lack of homologous Tet-family dioxygenase enzymes in Arabidopsis. ..
  14. pmc Beta-arrestin inhibits CAMKKbeta-dependent AMPK activation downstream of protease-activated-receptor-2
    Ping Wang
    Division of Biomedical Sciences University of California Riverside, California, USA
    BMC Biochem 11:36. 2010
    ....
  15. ncbi request reprint Fragmentation of deprotonated ions of oligodeoxynucleotides carrying a 5-formyluracil or 2-aminoimidazolone
    Yinsheng Wang
    Department of Chemistry, University of California at Riverside, 92521 0403, USA
    J Am Soc Mass Spectrom 13:1190-4. 2002
    ..In addition we observed that at high charge states, the loss of 5-formyluracil as an anion and the resulting strand cleavage is predominant over cleavages at other sites...
  16. ncbi request reprint The photochemistry of 5-BrdCpdC, dCp5-BrdCpdA, and dCp5-BrdCdT in aqueous solution
    Yuan Gao
    Department of Chemistry 027, University of California, 900 University Avenue, Riverside, CA 92521, USA
    Nucleosides Nucleotides Nucleic Acids 25:279-87. 2006
    ..The same cross-link product can also be induced from the similar UV irradiation of dCp5-BrdCpdA and dCp5-BrdCpdT...
  17. pmc Homeodomain-interacting protein kinase-2 (HIPK2) phosphorylates HMGA1a at Ser-35, Thr-52, and Thr-77 and modulates its DNA binding affinity
    Qingchun Zhang
    J Proteome Res 6:4711-9. 2007
    ....
  18. ncbi request reprint Fragmentation of protonated ions of peptides containing cysteine, cysteine sulfinic acid, and cysteine sulfonic acid
    Yinsheng Wang
    Department of Chemistry, University of California at Riveirside, Riverside, California 92521 0403, USA
    J Am Soc Mass Spectrom 15:697-702. 2004
    ..The results are important for the determination of the site of the cysteine oxidation and might be useful for the sequencing of cysteine-containing peptides...
  19. ncbi request reprint ESI-MS/MS for the differentiation of diastereomeric pyrimidine glycols in mononucleosides
    Yinsheng Wang
    Department of Chemistry, University of California at Riverside, 92521 0403, USA
    Anal Chem 74:4505-12. 2002
    ..MS/MS acquired at different collisional energies gave similar results, which establishes the reproducibility of spectra...
  20. doi request reprint Bulky DNA lesions induced by reactive oxygen species
    Yinsheng Wang
    Department of Chemistry 027, University of California, Riverside, CA 92521 0403, USA
    Chem Res Toxicol 21:276-81. 2008
    ..These bulky, oxidatively induced DNA lesions may contribute significantly to neurological disorders that are associated with deficiency in NER and the natural processes of aging...
  21. ncbi request reprint HPLC isolation and mass spectrometric characterization of two isomers of thymine glycols in oligodeoxynucleotides
    Yinsheng Wang
    Department of Chemistry 027, University of California at Riverside, 92521 0403, USA
    Chem Res Toxicol 15:671-6. 2002
    ..We further demonstrate that the [M + H]+ and [M + Na]+ ions of the two cis stereoisomers of thymine glycol in the dinucleotides, which are extricated from the ODNs by nuclease P1, gave distinctive product-ion spectra...
  22. pmc Quantification of oxidative DNA lesions in tissues of Long-Evans Cinnamon rats by capillary high-performance liquid chromatography-tandem mass spectrometry coupled with stable isotope-dilution method
    Jin Wang
    Department of Chemistry, University of California, Riverside, California 92521 0403, United States
    Anal Chem 83:2201-9. 2011
    ....
  23. pmc Efficient formation of the tandem thymine glycol/8-oxo-7,8-dihydroguanine lesion in isolated DNA and the mutagenic and cytotoxic properties of the tandem lesions in Escherichia coli cells
    Bifeng Yuan
    Department of Chemistry and, Environmental Toxicology Graduate Program, University of California, Riverside, California 92521 0403, USA
    Chem Res Toxicol 23:11-9. 2010
    ..coli cells. Together, our results support that complex lesions could exert greater cytotoxic and mutagenic effects than when the composing individual lesions are present alone...
  24. pmc Stereospecific synthesis and characterization of oligodeoxyribonucleotides containing an N2-(1-carboxyethyl)-2'-deoxyguanosine
    Huachuan Cao
    Department of Chemistry, University of California, Riverside, California 92521 0403, USA
    J Am Chem Soc 129:12123-30. 2007
    ..Strikingly, the polymerase incorporated incorrect nucleotides, dGMP and dAMP, opposite the lesion more preferentially than the correct nucleotide, dCMP...
  25. pmc Identification and quantification of a guanine-thymine intrastrand cross-link lesion induced by Cu(II)/H2O2/ascorbate
    Haizheng Hong
    Environmental Toxicology Graduate Program, University of California, Riverside, California 92521 0403, USA
    Chem Res Toxicol 19:614-21. 2006
    ..The induction of intrastrand cross-link lesion in calf thymus DNA by Fenton reagents in vitro suggests that this type of lesion might be formed endogenously in mammalian cells...
  26. pmc Efficient and accurate bypass of N2-(1-carboxyethyl)-2'-deoxyguanosine by DinB DNA polymerase in vitro and in vivo
    Bifeng Yuan
    Department of Chemistry and Environmental Toxicology Graduate Program, University of California, Riverside, CA 92521 0403, USA
    Proc Natl Acad Sci U S A 105:8679-84. 2008
    ..e., polymerase kappa), opposite the N(2)-CEdG is both accurate and efficient. Taken together, our data support that N(2)-CEdG, a minor-groove DNA adduct arising from MG, is an important endogenous substrate for DinB DNA polymerase...
  27. pmc Effects of 6-thioguanine and S6-methylthioguanine on transcription in vitro and in human cells
    Changjun You
    Department of Chemistry, University of California, Riverside, California 92521 0403, USA
    J Biol Chem 287:40915-23. 2012
    ..We propose that S(6)mG might contribute, at least in part, to thiopurine-mediated cytotoxicity through inhibition of transcription and to potential therapy-related carcinogenesis via transcriptional mutagenesis...
  28. pmc Accurate and efficient bypass of 8,5'-cyclopurine-2'-deoxynucleosides by human and yeast DNA polymerase η
    Ashley L Swanson
    Environmental Toxicology Graduate Program, University of California, Riverside, CA 92521 0403, USA
    Chem Res Toxicol 25:1682-91. 2012
    ..Together, our results suggested that pol η may have an additional function in cells, i.e., to alleviate the cellular burden of endogenously induced DNA lesions, including cyclo-dA and cyclo-dG...
  29. pmc Replication across regioisomeric ethylated thymidine lesions by purified DNA polymerases
    Nisana Andersen
    Department of Chemistry and Environmental Toxicology Graduate Program, University of California, Riverside, California 92521 0403, United States
    Chem Res Toxicol 26:1730-8. 2013
    ..Thus, our results suggested that O(2)-EtdT and N3-EtdT may also contribute to the AT → TA and AT → GC mutations observed in cells and tissues of animals exposed to ethylating agents. ..
  30. ncbi request reprint Facile formation of an intrastrand cross-link lesion between cytosine and guanine upon pyrex-filtered UV light irradiation of d((Br)CG) and duplex DNA containing 5-bromocytosine
    Yu Zeng
    Department of Chemistry 027, University of California at Riverside, Riverside, California 92521 0403, USA
    J Am Chem Soc 126:6552-3. 2004
    ..A similar cross-link lesion has been previously found in the X-ray irradiation mixture of d(CGTA)...
  31. ncbi request reprint Formation of intrastrand cross-link products between cytosine and adenine from UV irradiation of d((Br)CA) and duplex DNA containing a 5-bromocytosine
    Haizheng Hong
    Department of Chemistry and Environmental Toxicology Graduate Program, University of California, Riverside, California 92521 0403, USA
    J Am Chem Soc 127:13969-77. 2005
    ..The chemistry discovered here may facilitate the future preparations of oxidative cross-link lesion-bearing substrates for biochemical and biophysical studies...
  32. pmc In-vitro replication studies on O(2)-methylthymidine and O(4)-methylthymidine
    Nisana Andersen
    Department of Chemistry, University of California, Riverside, CA 92521 0403, USA
    Chem Res Toxicol 25:2523-31. 2012
    ..In addition, the results from our study revealed that, if left unrepaired, O-methylated thymidine lesions may constitute important sources of nucleobase substitutions emanating from exposure to alkylating agents...
  33. pmc Quantitative analysis of DNA interstrand cross-links and monoadducts formed in human cells induced by psoralens and UVA irradiation
    Congfang Lai
    Department of Chemistry, University of California, Riverside, California 92521 0403, USA
    Anal Chem 80:8790-8. 2008
    ....
  34. ncbi request reprint Isolation and characterization of a novel cross-link lesion in d(CpC) induced by one-electron photooxidation
    Zhenjiu Liu
    Department of Chemistry 027, University of California Riverside, Riverside, CA 92501 0403, USA
    Photochem Photobiol 80:209-15. 2004
    ..In addition, the 5' cytosine moiety is deaminated and the C5 carbon atom in this cytosine is oxidized to a carbonyl group...
  35. pmc Global proteome quantification for discovering imatinib-induced perturbation of multiple biological pathways in K562 human chronic myeloid leukemia cells
    Lei Xiong
    Department of Chemistry, University of California, Riverside, CA 92521 0403, USA
    J Proteome Res 9:6007-15. 2010
    ..Overall, these results provided potential biomarkers for monitoring the therapeutic intervention of CML using imatinib and offered important new knowledge for gaining insight into the molecular mechanisms of action of imatinib...
  36. ncbi request reprint The reactivity of the 5-hydroxy-5,6-dihydrothymidin-6-yl radical in oligodeoxyribonucleotides
    Qibin Zhang
    Department of Chemistry 027, University of California at Riverside, Riverside, California 92521 0403, USA
    Chem Res Toxicol 18:1897-906. 2005
    ..The formation of this type of cross-link product in duplex ODNs from either the independently generated radical or gamma irradiation was near or below the detection limit of the LC-MS/MS method that we used...
  37. ncbi request reprint Mechanisms for the formation of major oxidation products of adenine upon 365-nm irradiation with 2-methyl-1,4-naphthoquinone as a sensitizer
    Yinsheng Wang
    Department of Chemistry 027, University of California at Riverside, Riverside, California 92521 0403, USA
    J Org Chem 67:8507-12. 2002
    ..From above results, we proposed mechanisms for the formation of the two products...
  38. pmc In vitro replication and repair studies of tandem lesions containing neighboring thymidine glycol and 8-oxo-7,8-dihydro-2'-deoxyguanosine
    Yong Jiang
    Environmental Toxicology Graduate Program and Department of Chemistry, University of California, Riverside, California 92521 0403, USA
    Chem Res Toxicol 22:574-83. 2009
    ..These results supported the notion that the replication and repair of tandem single-nucleobase lesions depend on the types of lesions involved and their spatial arrangement...
  39. ncbi request reprint In vivo formation and in vitro replication of a guanine-thymine intrastrand cross-link lesion
    Yong Jiang
    Environmental Toxicology Graduate Program, University of California at Riverside, Riverside, California 92521, USA
    Biochemistry 46:12757-63. 2007
    ..The above results support the conclusion that oxidative intrastrand cross-link lesions, if not repaired, can be cytotoxic and mutagenic...
  40. ncbi request reprint Thermodynamic and in vitro replication studies of an intrastrand G[8-5]C cross-link lesion
    Chunang Gu
    Environmental Toxicology Graduate Program and Department of Chemistry 027, University of California, Riverside, California 92521 0403, USA
    Biochemistry 44:8883-9. 2005
    ..The G[8-5]C lesion destabilizes the duplex form by approximately 4 kcal/mol in free energy at 25 degrees C relative to the undamaged parent duplex, and the thermally most stable duplex has natural bases opposite the lesion...
  41. pmc 5-Aza-2'-deoxycytidine induced growth inhibition of leukemia cells through modulating endogenous cholesterol biosynthesis
    Fan Zhang
    Department of Chemistry, University of California, Riverside, California 92521 0403, USA
    Mol Cell Proteomics 11:M111.016915. 2012
    ....
  42. pmc Isotope-coded ATP probe for quantitative affinity profiling of ATP-binding proteins
    Yongsheng Xiao
    Department of Chemistry, University of California, Riverside, California 92521 0403, United States
    Anal Chem 85:7478-86. 2013
    ..The ICAP approach described in the present paper should be generally applicable for the quantitative assessment of ATP-binding proteins in proteomic samples from cells and tissues. ..
  43. ncbi request reprint Independent generation of the 5-hydroxy-5,6-dihydrothymidin-6-yl radical and its reactivity in dinucleoside monophosphates
    Qibin Zhang
    Department of Chemistry 027, University of California at Riverside, Riverside, California 92521 0403, USA
    J Am Chem Soc 126:13287-97. 2004
    ..Moreover, LC-MS/MS results showed that gamma-radiation of d(GT) under anaerobic condition yielded the same type of cross-link lesions...
  44. ncbi request reprint Independent generation of 5-(2'-deoxycytidinyl)methyl radical and the formation of a novel cross-link lesion between 5-methylcytosine and guanine
    Qibin Zhang
    Department of Chemistry 027, University of California at Riverside, Riverside, California 92521 0403, USA
    J Am Chem Soc 125:12795-802. 2003
    ..Furthermore, we show that the cross-link lesion can also form in d(mCG) from gamma irradiation under anaerobic conditions...
  45. ncbi request reprint Tandem mass spectrometry for the examination of the posttranslational modifications of high-mobility group A1 proteins: symmetric and asymmetric dimethylation of Arg25 in HMGA1a protein
    Yan Zou
    Department of Chemistry 027, University of California at Riverside, Riverside, California 92521 0403, USA
    Biochemistry 44:6293-301. 2005
    ..The unambiguous identification of the sites of phosphorylation and the nature of methylation facilitates the future examination of the biological implications of the HMGA1 proteins...
  46. pmc Identification and characterization of a novel cross-link lesion in d(CpC) upon 365-nm irradiation in the presence of 2-methyl-1,4-naphthoquinone
    Zhenjiu Liu
    Department of Chemistry 027, University of California at Riverside, Riverside, CA 92521 0403, USA
    Nucleic Acids Res 31:5413-24. 2003
    ....
  47. pmc Site-specific effects of 17beta-estradiol in hornyhead turbot (Pleuronichthys verticalis) collected from a wastewater outfall and reference location
    Mary Ann Rempel-Hester
    Environmental Toxicology Graduate Program, University of California Riverside, Riverside, CA, USA
    Environ Res 109:552-8. 2009
    ....
  48. pmc A quantitative study on the in vitro and in vivo acetylation of high mobility group A1 proteins
    Qingchun Zhang
    Department of Chemistry 027, University of California, Riverside, California 92521 0403, USA
    J Am Soc Mass Spectrom 18:1569-78. 2007
    ..Our results showed that all the above five lysine residues were also acetylated in vivo, with Lys-64, Lys-66 and Lys-70 in HMGA1a exhibiting higher levels of acetylation than Lys-14 and Lys-73...
  49. pmc Monomethylarsonous acid inhibited endogenous cholesterol biosynthesis in human skin fibroblasts
    Lei Guo
    Environmental Toxicology Graduate Program, University of California, Riverside, CA 92521 0403, United States
    Toxicol Appl Pharmacol 277:21-9. 2014
    ..Collectively, the present study demonstrated that the cytotoxicity of MMA(III) may arise, at least in part, from the down-regulation of cholesterol biosynthesis enzymes and the resultant decrease of cellular cholesterol content...
  50. ncbi request reprint LC-MS/MS identification and yeast polymerase eta bypass of a novel gamma-irradiation-induced intrastrand cross-link lesion G[8-5]C
    Chunang Gu
    Environmental Toxicology Graduate Program, University of California, Riverside, California 92521 0403, USA
    Biochemistry 43:6745-50. 2004
    ..The above results support that the oxidative cross-link lesion, if not repaired, can be mutagenic...
  51. pmc Recognition and incision of oxidative intrastrand cross-link lesions by UvrABC nuclease
    Chunang Gu
    Environmental Toxicology Graduate Program, University of California, Riverside, California 92521 0403, USA
    Biochemistry 45:10739-46. 2006
    ..Taken together, the results from this study suggest that oxidative intrastrand lesions might be substrates for NER enzymes in vivo...
  52. pmc Quantitative assessment of Tet-induced oxidation products of 5-methylcytosine in cellular and tissue DNA
    Shuo Liu
    Environmental Toxicology Graduate Program, University of California, Riverside, CA 92521, USA
    Nucleic Acids Res 41:6421-9. 2013
    ....
  53. pmc Hexavalent chromium-induced alteration of proteomic landscape in human skin fibroblast cells
    Lei Guo
    Environmental Toxicology Graduate Program, University of California, Riverside, California 92521 0403, USA
    J Proteome Res 12:3511-8. 2013
    ....
  54. ncbi request reprint A mass spectrometric study on the in vitro methylation of HMGA1a and HMGA1b proteins by PRMTs: methylation specificity, the effect of binding to AT-rich duplex DNA, and the effect of C-terminal phosphorylation
    Yan Zou
    Department of Chemistry 027, University of California, Riverside, California 92521 0403, USA
    Biochemistry 46:7896-906. 2007
    ..Our results suggest that PRMT1 might be involved in the previously reported methylation of Arg25 in HMGA1a in vivo...
  55. pmc Fragmentation of isomeric intrastrand crosslink lesions of DNA in an ion-trap mass spectrometer
    Huachuan Cao
    Department of Chemistry, University of California at Riverside, Riverside, California 92521 0403, USA
    J Am Soc Mass Spectrom 20:611-7. 2009
    ..The results from the present study afforded important guidance for the application of mass spectrometry for the structure elucidation of other intrastrand/interstrand crosslink lesions...
  56. ncbi request reprint Mass spectrometric analysis of high-mobility group proteins and their post-translational modifications in normal and cancerous human breast tissues
    Yan Zou
    Department of Chemistry 027, University of California at Riverside, Riverside, California 92521 0403, USA
    J Proteome Res 6:2304-14. 2007
    ..Furthermore, a more complex spectrum of PTMs on HMGA1 proteins was correlated with a more aggressive malignancy in human breast cancer tissues...
  57. ncbi request reprint Collisionally activated dissociation of protonated 2'-deoxycytidine, 2'-deoxyuridine, and their oxidatively damaged derivatives
    Huachuan Cao
    Department of Chemistry, University of California, Riverside, California 92521 0403, USA
    J Am Soc Mass Spectrom 17:1335-41. 2006
    ..The results from this study provided a basis for the identifications of other modified pyrimidine bases/nucleosides by tandem mass spectrometry...
  58. pmc Identification of novel α-n-methylation of CENP-B that regulates its binding to the centromeric DNA
    Xiaoxia Dai
    Department of Chemistry, University of California, Riverside, California 92521 0403, United States
    J Proteome Res 12:4167-75. 2013
    ..Our study also expands the function of protein α-N-methylation that has been known for decades and whose function remains largely unexplored. ..
  59. pmc Proteome-wide discovery and characterizations of nucleotide-binding proteins with affinity-labeled chemical probes
    Yongsheng Xiao
    Department of Chemistry, University of California, Riverside, California 92521 0403, United States
    Anal Chem 85:3198-206. 2013
    ..Our strategy for capturing and characterizing ATP/GTP-binding proteins should be generally applicable for those proteins that can interact with other nucleotides...
  60. pmc Nuclease digestion and mass spectrometric characterization of oligodeoxyribonucleotides containing 1,2-GpG, 1,2-ApG, and 1,3-GpXpG cisplatin intrastrand cross-links
    Renee T Williams
    Department of Chemistry 027, University of California, Riverside, CA 92521 0403, United States
    Clin Chim Acta 420:160-70. 2013
    ..The primary mode of action for cis-diamminedichloroplatinum (II), referred to as cisplatin, toward the treatment of solid malignancies is through formation of cross-links with DNA at purine sites, especially guanines...
  61. pmc Quantitative proteomic analysis revealed lovastatin-induced perturbation of cellular pathways in HL-60 cells
    Xiaoli Dong
    Department of Chemistry, University of California, Riverside, California 92521 0403, United States
    J Proteome Res 10:5463-71. 2011
    ..k.a. carbonic anhydrase II) and perturbed the protein ubiquitination pathway. Together, the results from the present study underscored several new cellular pathways perturbed by lovastatin...
  62. ncbi request reprint Tandem mass spectrometry for the determination of the sites of DNA interstrand cross-link
    Yuesong Wang
    Department of Chemistry, University of California at Riverside, Riverside, California 92521 0403, USA
    J Am Soc Mass Spectrom 15:1565-71. 2004
    ....
  63. pmc LC-MS/MS coupled with stable isotope dilution method for the quantification of 6-thioguanine and S(6)-methylthioguanine in genomic DNA of human cancer cells treated with 6-thioguanine
    Hongxia Wang
    Department of Chemistry, University of California, Riverside, California 92521 0403, USA
    Anal Chem 82:5797-803. 2010
    ..In addition, our results suggested that DNA (S)G might trigger mismatch repair (MMR) pathway without being converted to S(6)mG...
  64. ncbi request reprint Phosphorylation of human high mobility group N1 protein by protein kinase CK2
    Xinzhao Grace Jiang
    Department of Chemistry 027, University of California, Riverside, 92521 0403, USA
    Biochem Biophys Res Commun 345:1497-503. 2006
    ..All five sites were previously shown to be phosphorylated in MCF-7 human breast cancer cells in vivo. Among these five sites, Ser6, Ser7, and Ser85 were new sites of phosphorylation induced by protein kinase CK2 in vitro...
  65. doi request reprint LC-MS/MS for the detection of DNA interstrand cross-links formed by 8-methoxypsoralen and UVA irradiation in human cells
    Huachuan Cao
    Department of Chemistry, University of California, Riverside, California 92521 0403, USA
    Anal Chem 80:2932-8. 2008
    ..The specificity and accuracy of this high-throughput approach are advantageous over other methods for the detection of ICLs formed in vitro and in vivo...
  66. pmc Facile photocyclization chemistry of 5-phenylthio-2'-deoxyuridine in duplex DNA
    Yu Zeng
    Department of Chemistry, University of California, Riverside, CA 92521 0403, USA
    Org Lett 8:2527-30. 2006
    ..The chemistry reported here may serve as the basis for the efficient preparation of a new class of duplex DNA with an extended pi system. [reaction: see text]..
  67. pmc Methyl group migration during the fragmentation of singly charged ions of trimethyllysine-containing peptides: precaution of using MS/MS of singly charged ions for interrogating peptide methylation
    Lei Xiong
    Department of Chemistry, University of California, Riverside, California, USA
    J Am Soc Mass Spectrom 20:1172-81. 2009
    ..The results presented in this study highlighted that caution should be exerted while MS/MS of singly charged ions is employed to interrogate the PTMs of trimethyllysine-containing peptides...
  68. ncbi request reprint Redox-dependent formation of disulfide bonds in human replication protein A
    Lijie Men
    Department of Chemistry 027, University of California, Riverside, CA 92521 0403, USA
    Rapid Commun Mass Spectrom 21:2743-9. 2007
    ..Moreover, the other 11 cysteines in this protein remained intact. The results demonstrated that the formation of disulfide bonds at the zinc-finger site was responsible for the redox regulation of the DNA-binding activity of RPA...
  69. pmc Conformation-dependent formation of the G[8-5]U intrastrand cross-link in 5-bromouracil-containing G-quadruplex DNA induced by UVA irradiation
    Guangxin Lin
    Department of Chemistry, University of California, Riverside, California 92521 0403, USA
    Biochemistry 49:2346-50. 2010
    ....
  70. ncbi request reprint The oxidation of yeast alcohol dehydrogenase-1 by hydrogen peroxide in vitro
    Lijie Men
    Department of Chemistry 027, University of California, Riverside, California 92521 0403, USA
    J Proteome Res 6:216-25. 2007
    ..The oxidation of Cys43 might be responsible for the inactivation of the enzyme upon H2O2 treatment...
  71. pmc In vitro replication studies of carboxymethylated DNA lesions with Saccharomyces cerevisiae polymerase η
    Ashley L Swanson
    Environmental Toxicology Graduate Program, University of California, Riverside, California 92521 0403, United States
    Biochemistry 50:7666-73. 2011
    ..These results suggest that these lesions may contribute significantly to diazoacetate-induced mutations and those in the p53 gene observed in human gastrointestinal tumors...
  72. pmc 6-Thioguanine reactivates epigenetically silenced genes in acute lymphoblastic leukemia cells by facilitating proteasome-mediated degradation of DNMT1
    Bifeng Yuan
    Department of Chemistry, University of California, Riverside, California 92521 0403, USA
    Cancer Res 71:1904-11. 2011
    ..Taken together, our findings reveal a previously uncharacterized but vital mechanistic link between (S)G treatment and DNA hypomethylation...
  73. ncbi request reprint Fragmentation of the deprotonated ions of peptides containing cysteine, cysteine sulfinic acid, cysteine sulfonic acid, aspartic acid, and glutamic acid
    Lijie Men
    Department of Chemistry 027, University of California at Riverside, Riverside, CA 92521 0403, USA
    Rapid Commun Mass Spectrom 20:777-84. 2006
    ..Furthermore, ab initio calculation results for model cleavage reactions are consistent with the selective cleavage of the backbone N-Calpha bond in the Asp residue...
  74. pmc Probing adenosine nucleotide-binding proteins with an affinity-labeled nucleotide probe and mass spectrometry
    Haibo Qiu
    Department of Chemistry 027, University of California, Riverside, California 92521 0403, USA
    Anal Chem 79:5547-56. 2007
    ....
  75. pmc Synthesis and thermodynamic studies of oligodeoxyribonucleotides containing tandem lesions of thymidine glycol and 8-oxo-2'-deoxyguanosine
    Yuesong Wang
    Department of Chemistry, University of California at Riverside, 92521 0403, USA
    Chem Res Toxicol 19:837-43. 2006
    ..The thermodynamic results could offer a basis for understanding the repair of the tandem base lesions...
  76. pmc 6-Thioguanine perturbs cytosine methylation at the CpG dinucleotide site by DNA methyltransferases in vitro and acts as a DNA demethylating agent in vivo
    Hongxia Wang
    Department of Chemistry, University of California, Riverside, California 92521 0403, USA
    Biochemistry 48:2290-9. 2009
    ..These results showed that 6-thioguanine, after being incorporated into DNA, may perturb the epigenetic pathway of gene regulation...
  77. pmc Quantification of N2-carboxymethyl-2'-deoxyguanosine in calf thymus DNA and cultured human kidney epithelial cells by capillary high-performance liquid chromatography-tandem mass spectrometry coupled with stable isotope dilution method
    Hongxia Wang
    Department of Chemistry, University of California, Riverside, California 92521 0403, USA
    Chem Res Toxicol 23:74-81. 2010
    ..25 mM. Collectively, our results suggested that N(2)-CMdG might serve as a biomarker for glyoxal exposure...
  78. ncbi request reprint UVB-Induced formation of intrastrand cross-link products of DNA in MCF-7 cells treated with 5-bromo-2'-deoxyuridine
    Yu Zeng
    Department of Chemistry 027, University of California, Riverside, California 92521 0403, USA
    Biochemistry 46:8189-95. 2007
    ..The formation of these intrastrand cross-link products and 8-oxo-dG in vivo underscores the importance of these products in the photosensitizing effect of BrdU...
  79. pmc Mapping of lysine methylation and acetylation in core histones of Neurospora crassa
    Lei Xiong
    Department of Chemistry, University of California, Riverside, California 92521 0403, USA
    Biochemistry 49:5236-43. 2010
    ..Our analysis provides a potentially comprehensive picture of methylation and acetylation of core histones in Neurospora, which should serve as a foundation for future studies of the function of histone PTMs in this model organism...
  80. pmc High mobility group proteins and their post-translational modifications
    Qingchun Zhang
    Department of Chemistry, University of California, Riverside, CA 92521 0403, USA
    Biochim Biophys Acta 1784:1159-66. 2008
    ..Here, we reviewed the identified covalent modifications of HMG proteins, and highlighted how these PTMs affect the functions of HMG proteins in a variety of cellular processes...
  81. pmc The roles of DNA polymerases κ and ι in the error-free bypass of N2-carboxyalkyl-2'-deoxyguanosine lesions in mammalian cells
    Bifeng Yuan
    From the Department of Chemistry, University of California, Riverside, California 92521, USA
    J Biol Chem 286:17503-11. 2011
    ..However, in the absence of polymerase κ or ι, other translesion synthesis polymerase(s) could incorporate nucleotide(s) opposite these lesions but would do so inaccurately...
  82. pmc Mutagenic and cytotoxic properties of 6-thioguanine, S6-methylthioguanine, and guanine-S6-sulfonic acid
    Bifeng Yuan
    Department of Chemistry, University of California, Riverside, CA 92521 0403, USA
    J Biol Chem 283:23665-70. 2008
    ..The mutagenic properties of 6-thioguanine and its derivatives revealed in the present study offered important knowledge about the biological implications of these thionucleosides...
  83. ncbi request reprint Major adenine products from 2-methyl-1,4-naphthoquinone-sensitized photoirradiation at 365 nm
    Yinsheng Wang
    Department of Chemistry, 027, University of California at Riverside, Riverside, CA 92521 0403, USA
    Biochem Biophys Res Commun 291:1252-7. 2002
    ..Our results are consistent with previous observation showing that N(6) radical formed on adenine upon UVA irradiation...
  84. ncbi request reprint Further studies on the fragmentation of protonated ions of peptides containing aspartic acid, glutamic acid, cysteine sulfinic acid, and cysteine sulfonic acid
    Lijie Men
    Department of Chemistry 027, University of California, Riverside, CA 92521 0403, USA
    Rapid Commun Mass Spectrom 19:23-30. 2005
    ..The results from this study may provide useful tools for peptide sequencing...
  85. ncbi request reprint Structure elucidation of DNA interstrand cross-link by a combination of nuclease P1 digestion with mass spectrometry
    Yuesong Wang
    Department of Chemistry 027, University of California at Riverside, Riverside, California 92521 0403, USA
    Anal Chem 75:6306-13. 2003
    ..We believe that the method described in this paper can be generally applicable for investigating the structures of other DNA ICLs...
  86. ncbi request reprint Identification of novel in vivo phosphorylation sites in high mobility group N1 protein from the MCF-7 human breast cancer cells
    Yan Zou
    Department of Chemistry 027, University of California at Riverside, Riverside, California 92521 0403, USA
    Biochemistry 43:6322-9. 2004
    ..In addition, the phosphorylation of HMGN1 at Ser85, which precedes Pro86, might play an important role in cellular signaling...
  87. ncbi request reprint Statins activate AMP-activated protein kinase in vitro and in vivo
    Wei Sun
    Division of Biomedical Sciences, University of California, Riverside, Riverside, CA 92521 0121, USA
    Circulation 114:2655-62. 2006
    ..We therefore studied whether statins can activate AMPK, and if so, whether the activated AMPK regulates nitric oxide (NO) production and angiogenesis mediated by endothelial NO synthase, a substrate of AMPK in vascular endothelial cells...
  88. pmc A method to determine the ionization efficiency change of peptides caused by phosphorylation
    Yuan Gao
    Department of Chemistry 027, University of California at Riverside, Riverside, California 92521 0403, USA
    J Am Soc Mass Spectrom 18:1973-6. 2007
    ..We demonstrated the usefulness of the method by assessing the change in ionization efficiencies of four peptides introduced by phosphorylation...
  89. pmc Uptake of estradiol from sediment by hornyhead turbot (Pleuronichthys verticalis) and effects on oxidative DNA damage in male gonads
    Mary Ann Rempel
    Environmental Toxicology Program, University of California Riverside, Riverside, CA, USA
    Mar Environ Res 66:111-2. 2008
    ..The results suggest that the DNA damage previously seen in the gonads of feral hornyhead turbot at a sewage outfall is likely not caused by acute exposure to exogenous E2 from sediments...
  90. ncbi request reprint Acetylation and phosphorylation of high-mobility group A1 proteins in PC-3 human tumor cells
    Xinzhao Jiang
    Department of Chemistry, 027, University of California at Riverside, Riverside, California 92521 0403, USA
    Biochemistry 45:7194-201. 2006
    ..In addition, the specifically posttranslationally modified HMGA1 proteins may serve as molecular biomarkers for cancer diagnosis and prognosis...
  91. pmc Redox reactions of copper complexes formed with different beta-amyloid peptides and their neuropathological [correction of neuropathalogical] relevance
    Dianlu Jiang
    Department of Chemistry and Biochemistry, California State University, Los Angeles, Los Angeles, California 90032, USA
    Biochemistry 46:9270-82. 2007
    ....
  92. doi request reprint Quantitative analysis of surface plasma membrane proteins of primary and metastatic melanoma cells
    Haibo Qiu
    Department of Chemistry, University of California, Riverside, CA 92521 0403, USA
    J Proteome Res 7:1904-15. 2008
    ....
  93. pmc Derivatization with Girard reagent T combined with LC-MS/MS for the sensitive detection of 5-formyl-2'-deoxyuridine in cellular DNA
    Haizheng Hong
    Environmental Toxicology Graduate Program and Department of Chemistry, University of California at Riverside, Riverside, California 92521, USA
    Anal Chem 79:322-6. 2007
    ..By using isotope-labeled FodU as the internal standard and this derivatization method, we further quantified, by using LC-MS/MS, the yield of FodU formed in cellular DNA...
  94. ncbi request reprint Beta-elimination coupled with tandem mass spectrometry for the identification of in vivo and in vitro phosphorylation sites in maize dehydrin DHN1 protein
    Xinzhao Jiang
    Department of Chemistry, Mail Drop 027, University of California, Riverside, California 92521 0403, USA
    Biochemistry 43:15567-76. 2004
    ..Moreover, the in vitro phosphorylation also occurred on the serine residues in the serine tract region, suggesting that CK2 might be involved in the phosphorylation of the serine track region in maize kernel in vivo...
  95. ncbi request reprint Site-selective modifications of arginine residues in human hemoglobin induced by methylglyoxal
    Yuan Gao
    Department of Chemistry 027, University of California, Riverside, California 92521 0403, USA
    Biochemistry 45:15654-60. 2006
    ....

Research Grants23

  1. ROS-induced Nucleic Acid Damage
    Yinsheng Wang; Fiscal Year: 2002
    ..The outcome of the research proposed here will provide better understanding of the biological consequences of oxidative damage on nucleic acids by ROS and their implications in human health. ..
  2. Post-translational Modifications of High-mobility Group Proteins
    Yinsheng Wang; Fiscal Year: 2009
    ..In addition, the outcome of proposed studies may provide important molecular biomarkers for cancer diagnosis and prognosis. ..
  3. Chemistry and Biology of DNA Carboxyalkylation
    Yinsheng Wang; Fiscal Year: 2009
    ..and exposure to N-nitroso compounds in the etiology of diabetic complications and gastrointestinal tumors, and lead to the discovery of novel molecular biomarkers and risk factors for developing these pathological conditions ..
  4. Chemistry and Biology of DNA Carboxyalkylation
    Yinsheng Wang; Fiscal Year: 2010
    ..and exposure to N-nitroso compounds in the etiology of diabetic complications and gastrointestinal tumors, and lead to the discovery of novel molecular biomarkers and risk factors for developing these pathological conditions ..
  5. Post-translational Modifications of High-mobility Group Proteins
    Yinsheng Wang; Fiscal Year: 2009
    ..In addition, the outcome of proposed studies may provide important molecular biomarkers for cancer diagnosis and prognosis. ..
  6. Chemistry and Biology of DNA Carboxyalkylation
    Yinsheng Wang; Fiscal Year: 2009
    ..and exposure to N-nitroso compounds in the etiology of diabetic complications and gastrointestinal tumors, and lead to the discovery of novel molecular biomarkers and risk factors for developing these pathological conditions ..
  7. Oxidative Crosslink Lesions and CpG Mutagenesis
    Yinsheng Wang; Fiscal Year: 2006
    ..The outcome of the proposed research will provide important insights into the roles of these crosslink lesions in CpG mutagenesis. ..
  8. Oxidative Crosslink Lesions and CpG Mutagenesis
    Yinsheng Wang; Fiscal Year: 2007
    ..The outcome of the proposed research will provide important insights into the roles of these crosslink lesions in CpG mutagenesis. ..
  9. Post-translational Modifications of High-mobility Group Proteins
    Yinsheng Wang; Fiscal Year: 2007
    ..In addition, the outcome of proposed studies may provide important molecular biomarkers for cancer diagnosis and prognosis. ..
  10. ROS-induced Nucleic Acid Damage
    Yinsheng Wang; Fiscal Year: 2003
    ..The outcome of the research proposed here will provide better understanding of the biological consequences of oxidative damage on nucleic acids by ROS and their implications in human health. ..
  11. ROS-induced Nucleic Acid Damage
    Yinsheng Wang; Fiscal Year: 2004
    ..The outcome of the research proposed here will provide better understanding of the biological consequences of oxidative damage on nucleic acids by ROS and their implications in human health. ..
  12. Oxidative Crosslink Lesions and CpG Mutagenesis
    Yinsheng Wang; Fiscal Year: 2004
    ..The outcome of the proposed research will provide important insights into the roles of these crosslink lesions in CpG mutagenesis. ..
  13. ROS-induced Nucleic Acid Damage
    Yinsheng Wang; Fiscal Year: 2004
    ..The outcome of the research proposed here will provide better understanding of the biological consequences of oxidative damage on nucleic acids by ROS and their implications in human health. ..
  14. ROS-induced Nucleic Acid Damage
    Yinsheng Wang; Fiscal Year: 2005
    ..The outcome of the research proposed here will provide better understanding of the biological consequences of oxidative damage on nucleic acids by ROS and their implications in human health. ..
  15. Oxidative Crosslink Lesions and CpG Mutagenesis
    Yinsheng Wang; Fiscal Year: 2005
    ..The outcome of the proposed research will provide important insights into the roles of these crosslink lesions in CpG mutagenesis. ..
  16. ROS-induced Nucleic Acid Damage
    Yinsheng Wang; Fiscal Year: 2006
    ..The outcome of the research proposed here will provide better understanding of the biological consequences of oxidative damage on nucleic acids by ROS and their implications in human health. ..
  17. Oxidative Crosslink Lesions and CpG Mutagenesis
    Yinsheng Wang; Fiscal Year: 2006
    ..The outcome of the proposed research will provide important insights into the roles of these crosslink lesions in CpG mutagenesis. ..
  18. Chemistry and Biology of DNA Carboxyalkylation
    Yinsheng Wang; Fiscal Year: 2010
    ..and exposure to N-nitroso compounds in the etiology of diabetic complications and gastrointestinal tumors, and lead to the discovery of novel molecular biomarkers and risk factors for developing these pathological conditions ..