Research Topics
Genomes and Genes | Yinsheng WangSummaryAffiliation: University of California Country: USA Publications
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Publications
Beta-arrestin inhibits CAMKKbeta-dependent AMPK activation downstream of protease-activated-receptor-2Ping Wang
Division of Biomedical Sciences University of California Riverside, California, USA
BMC Biochem 11:36. 2010....
Bulky DNA lesions induced by reactive oxygen speciesYinsheng Wang
Department of Chemistry 027, University of California, Riverside, CA 92521 0403, USA
Chem Res Toxicol 21:276-81. 2008..These bulky, oxidatively induced DNA lesions may contribute significantly to neurological disorders that are associated with deficiency in NER and the natural processes of aging...- Homeodomain-interacting protein kinase-2 (HIPK2) phosphorylates HMGA1a at Ser-35, Thr-52, and Thr-77 and modulates its DNA binding affinityQingchun Zhang
J Proteome Res 6:4711-9. 2007.... 
The photochemistry of 5-BrdCpdC, dCp5-BrdCpdA, and dCp5-BrdCdT in aqueous solutionYuan Gao
Department of Chemistry 027, University of California, 900 University Avenue, Riverside, CA 92521, USA
Nucleosides Nucleotides Nucleic Acids 25:279-87. 2006..The same cross-link product can also be induced from the similar UV irradiation of dCp5-BrdCpdA and dCp5-BrdCpdT...- Fragmentation of protonated ions of peptides containing cysteine, cysteine sulfinic acid, and cysteine sulfonic acidYinsheng Wang
Department of Chemistry, University of California at Riveirside, Riverside, California 92521 0403, USA
J Am Soc Mass Spectrom 15:697-702. 2004..The results are important for the determination of the site of the cysteine oxidation and might be useful for the sequencing of cysteine-containing peptides... - ESI-MS/MS for the differentiation of diastereomeric pyrimidine glycols in mononucleosidesYinsheng Wang
Department of Chemistry, University of California at Riverside, 92521 0403, USA
Anal Chem 74:4505-12. 2002..MS/MS acquired at different collisional energies gave similar results, which establishes the reproducibility of spectra... - HPLC isolation and mass spectrometric characterization of two isomers of thymine glycols in oligodeoxynucleotidesYinsheng Wang
Department of Chemistry 027, University of California at Riverside, 92521 0403, USA
Chem Res Toxicol 15:671-6. 2002..We further demonstrate that the [M + H]+ and [M + Na]+ ions of the two cis stereoisomers of thymine glycol in the dinucleotides, which are extricated from the ODNs by nuclease P1, gave distinctive product-ion spectra... - Fragmentation of deprotonated ions of oligodeoxynucleotides carrying a 5-formyluracil or 2-aminoimidazoloneYinsheng Wang
Department of Chemistry, University of California at Riverside, 92521 0403, USA
J Am Soc Mass Spectrom 13:1190-4. 2002..In addition we observed that at high charge states, the loss of 5-formyluracil as an anion and the resulting strand cleavage is predominant over cleavages at other sites... - Endogenous formation and repair of oxidatively induced G[8-5 m]T intrastrand cross-link lesionJin Wang
Department of Chemistry, University of California, Riverside, CA 92521 0403, USA
Nucleic Acids Res 40:7368-74. 2012..Together, our studies established that d(G[8-5 m]T) can be induced endogenously in mammalian tissues and constitutes a substrate for NER in vivo... - Efficient formation of the tandem thymine glycol/8-oxo-7,8-dihydroguanine lesion in isolated DNA and the mutagenic and cytotoxic properties of the tandem lesions in Escherichia coli cellsBifeng Yuan
Department of Chemistry and, Environmental Toxicology Graduate Program, University of California, Riverside, California 92521 0403, USA
Chem Res Toxicol 23:11-9. 2010..coli cells. Together, our results support that complex lesions could exert greater cytotoxic and mutagenic effects than when the composing individual lesions are present alone... - Quantification of oxidative DNA lesions in tissues of Long-Evans Cinnamon rats by capillary high-performance liquid chromatography-tandem mass spectrometry coupled with stable isotope-dilution methodJin Wang
Department of Chemistry, University of California, Riverside, California 92521 0403, United States
Anal Chem 83:2201-9. 2011.... - Effects of 6-thioguanine and S6-methylthioguanine on transcription in vitro and in human cellsChangjun You
Department of Chemistry, University of California, Riverside, California 92521 0403, USA
J Biol Chem 287:40915-23. 2012..We propose that S(6)mG might contribute, at least in part, to thiopurine-mediated cytotoxicity through inhibition of transcription and to potential therapy-related carcinogenesis via transcriptional mutagenesis... - Accurate and efficient bypass of 8,5'-cyclopurine-2'-deoxynucleosides by human and yeast DNA polymerase ηAshley L Swanson
Environmental Toxicology Graduate Program, University of California, Riverside, CA 92521 0403, USA
Chem Res Toxicol 25:1682-91. 2012..Together, our results suggested that pol η may have an additional function in cells, i.e., to alleviate the cellular burden of endogenously induced DNA lesions, including cyclo-dA and cyclo-dG... - Stereospecific synthesis and characterization of oligodeoxyribonucleotides containing an N2-(1-carboxyethyl)-2'-deoxyguanosineHuachuan Cao
Department of Chemistry, University of California, Riverside, California 92521 0403, USA
J Am Chem Soc 129:12123-30. 2007..Strikingly, the polymerase incorporated incorrect nucleotides, dGMP and dAMP, opposite the lesion more preferentially than the correct nucleotide, dCMP... - Formation and genotoxicity of a guanine-cytosine intrastrand cross-link lesion in vivoHaizheng Hong
Environmental Toxicology Graduate Program, University of California, Riverside, CA 92521 0403, USA
Nucleic Acids Res 35:7118-27. 2007..7% G-->T and a 1.2% G-->C transversion mutations. DNA replication in E. coli hosts deficient in SOS-induced polymerases revealed that polymerase V was responsible for the error-prone translesion synthesis in vivo... - Kinetics of deamination and Cu(II)/H2O2/Ascorbate-induced formation of 5-methylcytosine glycol at CpG sites in duplex DNAHuachuan Cao
Department of Chemistry and Environmental Toxicology Graduate Program, University of California, Riverside, CA 92521 0403, USA
Nucleic Acids Res 37:6635-43. 2009..Together, our data support that the formation and deamination of 5-methylcytosine glycol may contribute significantly to the C -->T transition mutation at mCpG dinucleotide site... - A quantitative assay for assessing the effects of DNA lesions on transcriptionChangjun You
Department of Chemistry, University of California Riverside, Riverside, CA, USA
Nat Chem Biol 8:817-22. 2012..This newly developed CTAB assay should be generally applicable for quantitatively assessing how other DNA lesions affect DNA transcription in vitro and in cells... - Quantification of oxidative single-base and intrastrand cross-link lesions in unmethylated and CpG-methylated DNA induced by Fenton-type reagentsHuachuan Cao
Department of Chemistry 027, University of California, Riverside, CA 92521 0403, USA
Nucleic Acids Res 35:4833-44. 2007..These results suggest that oxidative intrastrand cross-link lesions formed at methylated-CpG sites may account for the previously reported mCG-->TT tandem double mutations induced by Fenton-type reagents... - Global proteome quantification for discovering imatinib-induced perturbation of multiple biological pathways in K562 human chronic myeloid leukemia cellsLei Xiong
Department of Chemistry, University of California, Riverside, CA 92521 0403, USA
J Proteome Res 9:6007-15. 2010..Overall, these results provided potential biomarkers for monitoring the therapeutic intervention of CML using imatinib and offered important new knowledge for gaining insight into the molecular mechanisms of action of imatinib... - Quantitative analysis of DNA interstrand cross-links and monoadducts formed in human cells induced by psoralens and UVA irradiationCongfang Lai
Department of Chemistry, University of California, Riverside, California 92521 0403, USA
Anal Chem 80:8790-8. 2008.... - Mechanisms for the formation of major oxidation products of adenine upon 365-nm irradiation with 2-methyl-1,4-naphthoquinone as a sensitizerYinsheng Wang
Department of Chemistry-027, University of California at Riverside, Riverside, California 92521-0403, USA
J Org Chem 67:8507-12. 2002..From above results, we proposed mechanisms for the formation of the two products... - In vitro replication and repair studies of tandem lesions containing neighboring thymidine glycol and 8-oxo-7,8-dihydro-2'-deoxyguanosineYong Jiang
Environmental Toxicology Graduate Program and Department of Chemistry, University of California, Riverside, California 92521 0403, USA
Chem Res Toxicol 22:574-83. 2009..These results supported the notion that the replication and repair of tandem single-nucleobase lesions depend on the types of lesions involved and their spatial arrangement... - Efficient and accurate bypass of N2-(1-carboxyethyl)-2'-deoxyguanosine by DinB DNA polymerase in vitro and in vivoBifeng Yuan
Department of Chemistry and Environmental Toxicology Graduate Program, University of California, Riverside, CA 92521 0403, USA
Proc Natl Acad Sci U S A 105:8679-84. 2008..e., polymerase kappa), opposite the N(2)-CEdG is both accurate and efficient. Taken together, our data support that N(2)-CEdG, a minor-groove DNA adduct arising from MG, is an important endogenous substrate for DinB DNA polymerase... - The reactivity of the 5-hydroxy-5,6-dihydrothymidin-6-yl radical in oligodeoxyribonucleotidesQibin Zhang
Department of Chemistry-027, University of California at Riverside, Riverside, California 92521-0403, USA
Chem Res Toxicol 18:1897-906. 2005..The formation of this type of cross-link product in duplex ODNs from either the independently generated radical or gamma irradiation was near or below the detection limit of the LC-MS/MS method that we used... - Isolation and characterization of a novel cross-link lesion in d(CpC) induced by one-electron photooxidationZhenjiu Liu
Department of Chemistry-027, University of California-Riverside, Riverside, CA 92501-0403, USA
Photochem Photobiol 80:209-15. 2004..In addition, the 5' cytosine moiety is deaminated and the C5 carbon atom in this cytosine is oxidized to a carbonyl group... - In vivo formation and in vitro replication of a guanine-thymine intrastrand cross-link lesionYong Jiang
Environmental Toxicology Graduate Program, University of California at Riverside, Riverside, California 92521, USA
Biochemistry 46:12757-63. 2007..The above results support the conclusion that oxidative intrastrand cross-link lesions, if not repaired, can be cytotoxic and mutagenic... - A quantitative study on the in vitro and in vivo acetylation of high mobility group A1 proteinsQingchun Zhang
Department of Chemistry 027, University of California, Riverside, California 92521 0403, USA
J Am Soc Mass Spectrom 18:1569-78. 2007..Our results showed that all the above five lysine residues were also acetylated in vivo, with Lys-64, Lys-66 and Lys-70 in HMGA1a exhibiting higher levels of acetylation than Lys-14 and Lys-73... - Site-specific effects of 17beta-estradiol in hornyhead turbot (Pleuronichthys verticalis) collected from a wastewater outfall and reference locationMary Ann Rempel-Hester
Environmental Toxicology Graduate Program, University of California Riverside, Riverside, CA, USA
Environ Res 109:552-8. 2009.... - Tandem mass spectrometry for the examination of the posttranslational modifications of high-mobility group A1 proteins: symmetric and asymmetric dimethylation of Arg25 in HMGA1a proteinYan Zou
Department of Chemistry 027, University of California at Riverside, Riverside, California 92521 0403, USA
Biochemistry 44:6293-301. 2005..The unambiguous identification of the sites of phosphorylation and the nature of methylation facilitates the future examination of the biological implications of the HMGA1 proteins... - Identification and characterization of a novel cross-link lesion in d(CpC) upon 365-nm irradiation in the presence of 2-methyl-1,4-naphthoquinoneZhenjiu Liu
Department of Chemistry-027, University of California at Riverside, Riverside, CA 92521-0403, USA
Nucleic Acids Res 31:5413-24. 2003.... - Formation of intrastrand cross-link products between cytosine and adenine from UV irradiation of d((Br)CA) and duplex DNA containing a 5-bromocytosineHaizheng Hong
Department of Chemistry and Environmental Toxicology Graduate Program, University of California, Riverside, California 92521-0403, USA
J Am Chem Soc 127:13969-77. 2005..The chemistry discovered here may facilitate the future preparations of oxidative cross-link lesion-bearing substrates for biochemical and biophysical studies... - 5-Aza-2'-deoxycytidine induced growth inhibition of leukemia cells through modulating endogenous cholesterol biosynthesisFan Zhang
Department of Chemistry, University of California, Riverside, California 92521 0403, USA
Mol Cell Proteomics 11:M111.016915. 2012.... - Fragmentation of isomeric intrastrand crosslink lesions of DNA in an ion-trap mass spectrometerHuachuan Cao
Department of Chemistry, University of California at Riverside, Riverside, California 92521 0403, USA
J Am Soc Mass Spectrom 20:611-7. 2009..The results from the present study afforded important guidance for the application of mass spectrometry for the structure elucidation of other intrastrand/interstrand crosslink lesions... - A mass spectrometric study on the in vitro methylation of HMGA1a and HMGA1b proteins by PRMTs: methylation specificity, the effect of binding to AT-rich duplex DNA, and the effect of C-terminal phosphorylationYan Zou
Department of Chemistry 027, University of California, Riverside, California 92521 0403, USA
Biochemistry 46:7896-906. 2007..Our results suggest that PRMT1 might be involved in the previously reported methylation of Arg25 in HMGA1a in vivo... - Synthesis and characterization of oligodeoxyribonucleotides containing a site-specifically incorporated N6-carboxymethyl-2'-deoxyadenosine or N4-carboxymethyl-2'-deoxycytidineJianshuang Wang
Department of Chemistry, University of California, Riverside, CA 92521 0403, USA
Nucleic Acids Res 38:6774-84. 2010..The formation and the mutagenic potential of N(6)-CMdA and N(4)-CMdC suggest that these lesions may bear important implications in the etiology of NOC-induced tumor development... - Mass spectrometric analysis of high-mobility group proteins and their post-translational modifications in normal and cancerous human breast tissuesYan Zou
Department of Chemistry 027, University of California at Riverside, Riverside, California 92521 0403, USA
J Proteome Res 6:2304-14. 2007..Furthermore, a more complex spectrum of PTMs on HMGA1 proteins was correlated with a more aggressive malignancy in human breast cancer tissues... - Collisionally activated dissociation of protonated 2'-deoxycytidine, 2'-deoxyuridine, and their oxidatively damaged derivativesHuachuan Cao
Department of Chemistry, University of California, Riverside, California 92521-0403, USA
J Am Soc Mass Spectrom 17:1335-41. 2006..The results from this study provided a basis for the identifications of other modified pyrimidine bases/nucleosides by tandem mass spectrometry... - Generation of 5-(2'-deoxycytidyl)methyl radical and the formation of intrastrand cross-link lesions in oligodeoxyribonucleotidesQibin Zhang
Department of Chemistry, University of California at Riverside Riverside, CA 92521-0403, USA
Nucleic Acids Res 33:1593-603. 2005.... - Identification and quantification of a guanine-thymine intrastrand cross-link lesion induced by Cu(II)/H2O2/ascorbateHaizheng Hong
Environmental Toxicology Graduate Program, University of California, Riverside, California 92521-0403, USA
Chem Res Toxicol 19:614-21. 2006..The induction of intrastrand cross-link lesion in calf thymus DNA by Fenton reagents in vitro suggests that this type of lesion might be formed endogenously in mammalian cells... - Independent generation of the 5-hydroxy-5,6-dihydrothymidin-6-yl radical and its reactivity in dinucleoside monophosphatesQibin Zhang
Department of Chemistry-027, University of California at Riverside, Riverside, California 92521-0403, USA
J Am Chem Soc 126:13287-97. 2004..Moreover, LC-MS/MS results showed that gamma-radiation of d(GT) under anaerobic condition yielded the same type of cross-link lesions... - Facile formation of an intrastrand cross-link lesion between cytosine and guanine upon pyrex-filtered UV light irradiation of d((Br)CG) and duplex DNA containing 5-bromocytosineYu Zeng
Department of Chemistry-027, University of California at Riverside, Riverside, California 92521-0403, USA
J Am Chem Soc 126:6552-3. 2004..A similar cross-link lesion has been previously found in the X-ray irradiation mixture of d(CGTA)... - Quantitative proteomic analysis revealed lovastatin-induced perturbation of cellular pathways in HL-60 cellsXiaoli Dong
Department of Chemistry, University of California, Riverside, California 92521 0403, United States
J Proteome Res 10:5463-71. 2011..k.a. carbonic anhydrase II) and perturbed the protein ubiquitination pathway. Together, the results from the present study underscored several new cellular pathways perturbed by lovastatin... - Tandem mass spectrometry for the determination of the sites of DNA interstrand cross-linkYuesong Wang
Department of Chemistry, University of California at Riverside, Riverside, California 92521 0403, USA
J Am Soc Mass Spectrom 15:1565-71. 2004.... - Methyl group migration during the fragmentation of singly charged ions of trimethyllysine-containing peptides: precaution of using MS/MS of singly charged ions for interrogating peptide methylationLei Xiong
Department of Chemistry, University of California, Riverside, California, USA
J Am Soc Mass Spectrom 20:1172-81. 2009..The results presented in this study highlighted that caution should be exerted while MS/MS of singly charged ions is employed to interrogate the PTMs of trimethyllysine-containing peptides... - The oxidation of yeast alcohol dehydrogenase-1 by hydrogen peroxide in vitroLijie Men
Department of Chemistry 027, University of California, Riverside, California 92521 0403, USA
J Proteome Res 6:216-25. 2007..The oxidation of Cys43 might be responsible for the inactivation of the enzyme upon H2O2 treatment... - LC-MS/MS for the detection of DNA interstrand cross-links formed by 8-methoxypsoralen and UVA irradiation in human cellsHuachuan Cao
Department of Chemistry, University of California, Riverside, California 92521 0403, USA
Anal Chem 80:2932-8. 2008..The specificity and accuracy of this high-throughput approach are advantageous over other methods for the detection of ICLs formed in vitro and in vivo... - Phosphorylation of human high mobility group N1 protein by protein kinase CK2Xinzhao Grace Jiang
Department of Chemistry 027, University of California, Riverside, 92521 0403, USA
Biochem Biophys Res Commun 345:1497-503. 2006..All five sites were previously shown to be phosphorylated in MCF-7 human breast cancer cells in vivo. Among these five sites, Ser6, Ser7, and Ser85 were new sites of phosphorylation induced by protein kinase CK2 in vitro... - High-throughput analysis of the mutagenic and cytotoxic properties of DNA lesions by next-generation sequencingBifeng Yuan
Department of Chemistry, University of California, Riverside, CA 92521 0403, USA
Nucleic Acids Res 39:5945-54. 2011..Taken together, the newly developed method provided a high-throughput and readily affordable method for assessing quantitatively how DNA lesions compromise the efficiency and fidelity of DNA replication in cells... - In vitro replication and thermodynamic studies of methylation and oxidation modifications of 6-thioguanineChunang Gu
Environmental Toxicology Graduate Program, University of California, Riverside, CA 92521 0403, USA
Nucleic Acids Res 35:3693-704. 2007..Taken together, the results from this study shed important new light on the biological implications of the two metabolites of 6-TG... - LC-MS/MS coupled with stable isotope dilution method for the quantification of 6-thioguanine and S(6)-methylthioguanine in genomic DNA of human cancer cells treated with 6-thioguanineHongxia Wang
Department of Chemistry, University of California, Riverside, California 92521 0403, USA
Anal Chem 82:5797-803. 2010..In addition, our results suggested that DNA (S)G might trigger mismatch repair (MMR) pathway without being converted to S(6)mG... - Redox-dependent formation of disulfide bonds in human replication protein ALijie Men
Department of Chemistry 027, University of California, Riverside, CA 92521 0403, USA
Rapid Commun Mass Spectrom 21:2743-9. 2007..Moreover, the other 11 cysteines in this protein remained intact. The results demonstrated that the formation of disulfide bonds at the zinc-finger site was responsible for the redox regulation of the DNA-binding activity of RPA... - Conformation-dependent formation of the G[8-5]U intrastrand cross-link in 5-bromouracil-containing G-quadruplex DNA induced by UVA irradiationGuangxin Lin
Department of Chemistry, University of California, Riverside, California 92521 0403, USA
Biochemistry 49:2346-50. 2010.... - Recognition and incision of oxidative intrastrand cross-link lesions by UvrABC nucleaseChunang Gu
Environmental Toxicology Graduate Program, University of California, Riverside, California 92521 0403, USA
Biochemistry 45:10739-46. 2006..Taken together, the results from this study suggest that oxidative intrastrand lesions might be substrates for NER enzymes in vivo... - Facile photocyclization chemistry of 5-phenylthio-2'-deoxyuridine in duplex DNAYu Zeng
Department of Chemistry, University of California, Riverside, CA 92521-0403, USA
Org Lett 8:2527-30. 2006..The chemistry reported here may serve as the basis for the efficient preparation of a new class of duplex DNA with an extended pi system. [reaction: see text].. - Quantification of N2-carboxymethyl-2'-deoxyguanosine in calf thymus DNA and cultured human kidney epithelial cells by capillary high-performance liquid chromatography-tandem mass spectrometry coupled with stable isotope dilution methodHongxia Wang
Department of Chemistry, University of California, Riverside, California 92521 0403, USA
Chem Res Toxicol 23:74-81. 2010..25 mM. Collectively, our results suggested that N(2)-CMdG might serve as a biomarker for glyoxal exposure... - 6-Thioguanine perturbs cytosine methylation at the CpG dinucleotide site by DNA methyltransferases in vitro and acts as a DNA demethylating agent in vivoHongxia Wang
Department of Chemistry, University of California, Riverside, California 92521 0403, USA
Biochemistry 48:2290-9. 2009..These results showed that 6-thioguanine, after being incorporated into DNA, may perturb the epigenetic pathway of gene regulation... - In vitro replication studies of carboxymethylated DNA lesions with Saccharomyces cerevisiae polymerase ηAshley L Swanson
Environmental Toxicology Graduate Program, University of California, Riverside, California 92521 0403, United States
Biochemistry 50:7666-73. 2011..These results suggest that these lesions may contribute significantly to diazoacetate-induced mutations and those in the p53 gene observed in human gastrointestinal tumors... - 6-Thioguanine reactivates epigenetically silenced genes in acute lymphoblastic leukemia cells by facilitating proteasome-mediated degradation of DNMT1Bifeng Yuan
Department of Chemistry, University of California, Riverside, California 92521 0403, USA
Cancer Res 71:1904-11. 2011..Taken together, our findings reveal a previously uncharacterized but vital mechanistic link between (S)G treatment and DNA hypomethylation... - Mapping of lysine methylation and acetylation in core histones of Neurospora crassaLei Xiong
Department of Chemistry, University of California, Riverside, California 92521 0403, USA
Biochemistry 49:5236-43. 2010..Our analysis provides a potentially comprehensive picture of methylation and acetylation of core histones in Neurospora, which should serve as a foundation for future studies of the function of histone PTMs in this model organism... - Synthesis and thermodynamic studies of oligodeoxyribonucleotides containing tandem lesions of thymidine glycol and 8-oxo-2'-deoxyguanosineYuesong Wang
Department of Chemistry, University of California at Riverside, 92521-0403, USA
Chem Res Toxicol 19:837-43. 2006..The thermodynamic results could offer a basis for understanding the repair of the tandem base lesions... - Fragmentation of the deprotonated ions of peptides containing cysteine, cysteine sulfinic acid, cysteine sulfonic acid, aspartic acid, and glutamic acidLijie Men
Department of Chemistry-027, University of California at Riverside, Riverside, CA 92521-0403, USA
Rapid Commun Mass Spectrom 20:777-84. 2006..Furthermore, ab initio calculation results for model cleavage reactions are consistent with the selective cleavage of the backbone N-Calpha bond in the Asp residue... - UVB-Induced formation of intrastrand cross-link products of DNA in MCF-7 cells treated with 5-bromo-2'-deoxyuridineYu Zeng
Department of Chemistry 027, University of California, Riverside, California 92521 0403, USA
Biochemistry 46:8189-95. 2007..The formation of these intrastrand cross-link products and 8-oxo-dG in vivo underscores the importance of these products in the photosensitizing effect of BrdU... - Probing adenosine nucleotide-binding proteins with an affinity-labeled nucleotide probe and mass spectrometryHaibo Qiu
Department of Chemistry 027, University of California, Riverside, California 92521 0403, USA
Anal Chem 79:5547-56. 2007.... - Sequence-dependent formation of intrastrand crosslink products from the UVB irradiation of duplex DNA containing a 5-bromo-2'-deoxyuridine or 5-bromo-2'-deoxycytidineYu Zeng
Department of Chemistry 027, University of California, Riverside, CA 92521 0403, USA
Nucleic Acids Res 34:6521-9. 2006..The formation of intrastrand crosslink products from these halopyrimidines in duplex DNA may account for the photosensitizing effects of these nucleosides... - Thermodynamic and in vitro replication studies of an intrastrand G[8-5]C cross-link lesionChunang Gu
Environmental Toxicology Graduate Program and Department of Chemistry-027, University of California, Riverside, California 92521-0403, USA
Biochemistry 44:8883-9. 2005..The G[8-5]C lesion destabilizes the duplex form by approximately 4 kcal/mol in free energy at 25 degrees C relative to the undamaged parent duplex, and the thermally most stable duplex has natural bases opposite the lesion... - High mobility group proteins and their post-translational modificationsQingchun Zhang
Department of Chemistry, University of California, Riverside, CA 92521 0403, USA
Biochim Biophys Acta 1784:1159-66. 2008..Here, we reviewed the identified covalent modifications of HMG proteins, and highlighted how these PTMs affect the functions of HMG proteins in a variety of cellular processes... - Mutagenic and cytotoxic properties of 6-thioguanine, S6-methylthioguanine, and guanine-S6-sulfonic acidBifeng Yuan
Department of Chemistry, University of California, Riverside, CA 92521 0403, USA
J Biol Chem 283:23665-70. 2008..The mutagenic properties of 6-thioguanine and its derivatives revealed in the present study offered important knowledge about the biological implications of these thionucleosides... - The roles of DNA polymerases κ and ι in the error-free bypass of N2-carboxyalkyl-2'-deoxyguanosine lesions in mammalian cellsBifeng Yuan
From the Department of Chemistry, University of California, Riverside, California 92521, USA
J Biol Chem 286:17503-11. 2011..However, in the absence of polymerase κ or ι, other translesion synthesis polymerase(s) could incorporate nucleotide(s) opposite these lesions but would do so inaccurately... - Independent generation of 5-(2'-deoxycytidinyl)methyl radical and the formation of a novel cross-link lesion between 5-methylcytosine and guanineQibin Zhang
Department of Chemistry-027, University of California at Riverside, Riverside, California 92521-0403, USA
J Am Chem Soc 125:12795-802. 2003..Furthermore, we show that the cross-link lesion can also form in d(mCG) from gamma irradiation under anaerobic conditions... - Major adenine products from 2-methyl-1,4-naphthoquinone-sensitized photoirradiation at 365 nmYinsheng Wang
Department of Chemistry, 027, University of California at Riverside, Riverside, CA 92521 0403, USA
Biochem Biophys Res Commun 291:1252-7. 2002..Our results are consistent with previous observation showing that N(6) radical formed on adenine upon UVA irradiation... - LC-MS/MS identification and yeast polymerase eta bypass of a novel gamma-irradiation-induced intrastrand cross-link lesion G[8-5]CChunang Gu
Environmental Toxicology Graduate Program, University of California, Riverside, California 92521-0403, USA
Biochemistry 43:6745-50. 2004..The above results support that the oxidative cross-link lesion, if not repaired, can be mutagenic... - Further studies on the fragmentation of protonated ions of peptides containing aspartic acid, glutamic acid, cysteine sulfinic acid, and cysteine sulfonic acidLijie Men
Department of Chemistry-027, University of California, Riverside, CA 92521-0403, USA
Rapid Commun Mass Spectrom 19:23-30. 2005..The results from this study may provide useful tools for peptide sequencing... - Structure elucidation of DNA interstrand cross-link by a combination of nuclease P1 digestion with mass spectrometryYuesong Wang
Department of Chemistry 027, University of California at Riverside, Riverside, California 92521 0403, USA
Anal Chem 75:6306-13. 2003..We believe that the method described in this paper can be generally applicable for investigating the structures of other DNA ICLs... - Chemical synthesis of oligodeoxyribonucleotides containing N3- and O4-carboxymethylthymidine and their formation in DNAJianshuang Wang
Department of Chemistry, University of California, Riverside, CA 92521 0403, USA
Nucleic Acids Res 37:336-45. 2009.... - Identification of novel in vivo phosphorylation sites in high mobility group N1 protein from the MCF-7 human breast cancer cellsYan Zou
Department of Chemistry 027, University of California at Riverside, Riverside, California 92521 0403, USA
Biochemistry 43:6322-9. 2004..In addition, the phosphorylation of HMGN1 at Ser85, which precedes Pro86, might play an important role in cellular signaling... - A method to determine the ionization efficiency change of peptides caused by phosphorylationYuan Gao
Department of Chemistry 027, University of California at Riverside, Riverside, California 92521 0403, USA
J Am Soc Mass Spectrom 18:1973-6. 2007..We demonstrated the usefulness of the method by assessing the change in ionization efficiencies of four peptides introduced by phosphorylation... - Statins activate AMP-activated protein kinase in vitro and in vivoWei Sun
Division of Biomedical Sciences, University of California, Riverside, Riverside, CA 92521-0121, USA
Circulation 114:2655-62. 2006..Such phosphorylation results in endothelial NO synthase activation, which provides a novel explanation for the pleiotropic effects of statins that benefit the cardiovascular system... - Uptake of estradiol from sediment by hornyhead turbot (Pleuronichthys verticalis) and effects on oxidative DNA damage in male gonadsMary Ann Rempel
Environmental Toxicology Program, University of California Riverside, Riverside, CA, USA
Mar Environ Res 66:111-2. 2008..The results suggest that the DNA damage previously seen in the gonads of feral hornyhead turbot at a sewage outfall is likely not caused by acute exposure to exogenous E2 from sediments... - Acetylation and phosphorylation of high-mobility group A1 proteins in PC-3 human tumor cellsXinzhao Jiang
Department of Chemistry, 027, University of California at Riverside, Riverside, California 92521-0403, USA
Biochemistry 45:7194-201. 2006..In addition, the specifically posttranslationally modified HMGA1 proteins may serve as molecular biomarkers for cancer diagnosis and prognosis... - Redox reactions of copper complexes formed with different beta-amyloid peptides and their neuropathological [correction of neuropathalogical] relevanceDianlu Jiang
Department of Chemistry and Biochemistry, California State University, Los Angeles, Los Angeles, California 90032, USA
Biochemistry 46:9270-82. 2007.... - Derivatization with Girard reagent T combined with LC-MS/MS for the sensitive detection of 5-formyl-2'-deoxyuridine in cellular DNAHaizheng Hong
Environmental Toxicology Graduate Program and Department of Chemistry, University of California at Riverside, Riverside, California 92521, USA
Anal Chem 79:322-6. 2007..By using isotope-labeled FodU as the internal standard and this derivatization method, we further quantified, by using LC-MS/MS, the yield of FodU formed in cellular DNA... - Site-selective modifications of arginine residues in human hemoglobin induced by methylglyoxalYuan Gao
Department of Chemistry-027, University of California, Riverside, California 92521-0403, USA
Biochemistry 45:15654-60. 2006.... - Beta-elimination coupled with tandem mass spectrometry for the identification of in vivo and in vitro phosphorylation sites in maize dehydrin DHN1 proteinXinzhao Jiang
Department of Chemistry, Mail Drop 027, University of California, Riverside, California 92521-0403, USA
Biochemistry 43:15567-76. 2004..Moreover, the in vitro phosphorylation also occurred on the serine residues in the serine tract region, suggesting that CK2 might be involved in the phosphorylation of the serine track region in maize kernel in vivo... - Quantitative analysis of surface plasma membrane proteins of primary and metastatic melanoma cellsHaibo Qiu
Department of Chemistry, University of California, Riverside, CA 92521 0403, USA
J Proteome Res 7:1904-15. 2008....
Research Grants
- Post-translational Modifications of High-mobility Group ProteinsYinsheng Wang; Fiscal Year: 2009..In addition, the outcome of proposed studies may provide important molecular biomarkers for cancer diagnosis and prognosis. ..
- Chemistry and Biology of DNA CarboxyalkylationYinsheng Wang; Fiscal Year: 2010..and exposure to N-nitroso compounds in the etiology of diabetic complications and gastrointestinal tumors, and lead to the discovery of novel molecular biomarkers and risk factors for developing these pathological conditions ..
- Post-translational Modifications of High-mobility Group ProteinsYinsheng Wang; Fiscal Year: 2009..In addition, the outcome of proposed studies may provide important molecular biomarkers for cancer diagnosis and prognosis. ..
- Oxidative Crosslink Lesions and CpG MutagenesisYinsheng Wang; Fiscal Year: 2007..The outcome of the proposed research will provide important insights into the roles of these crosslink lesions in CpG mutagenesis. ..
- ROS-induced Nucleic Acid DamageYinsheng Wang; Fiscal Year: 2006..The outcome of the research proposed here will provide better understanding of the biological consequences of oxidative damage on nucleic acids by ROS and their implications in human health. ..
- Chemistry and Biology of DNA CarboxyalkylationYinsheng Wang; Fiscal Year: 2010..and exposure to N-nitroso compounds in the etiology of diabetic complications and gastrointestinal tumors, and lead to the discovery of novel molecular biomarkers and risk factors for developing these pathological conditions ..