Yingxiao Wang

Summary

Affiliation: University of Illinois
Country: USA

Publications

  1. pmc FRET and mechanobiology
    Yingxiao Wang
    Department of Bioengineering, Center for Biophysics and Computational Biology, Institute of Genomic Biology, The Beckman Institute for Advance Science and Technology, University of Illinois at Urbana Champaign, IL 61801, USA
    Integr Biol (Camb) 1:565-73. 2009
  2. doi request reprint Fluorescence proteins, live-cell imaging, and mechanobiology: seeing is believing
    Yingxiao Wang
    Department of Bioengineering and Beckman Institute for Advanced Science and Technology, University of Illinois, Urbana Champaign, Urbana, IL 61801, USA
    Annu Rev Biomed Eng 10:1-38. 2008
  3. pmc Two distinct phases of calcium signalling under flow
    Bo Liu
    Department of Bioengineering and Beckman Institute for Advanced Science and Technology, University of Illinois, Urbana Champaign, Urbana, IL 61801, USA
    Cardiovasc Res 91:124-33. 2011
  4. pmc Plectin contributes to mechanical properties of living cells
    Sungsoo Na
    Dept of Mechanical Science, Univ of Illinois, Urbana, IL 61801, USA
    Am J Physiol Cell Physiol 296:C868-77. 2009
  5. pmc Visualization of Src activity at different compartments of the plasma membrane by FRET imaging
    Jihye Seong
    Neuroscience Program, Center for Biophysics and Computational Biology, University of Illinois at Urbana Champaign, Urbana, IL 61801, USA
    Chem Biol 16:48-57. 2009
  6. pmc Substrate rigidity regulates Ca2+ oscillation via RhoA pathway in stem cells
    Tae Jin Kim
    Neuroscience Program, Beckman Institute for Advanced Science and Technology, University of Illinois, Urbana Champaign, Urbana, Illinois, USA
    J Cell Physiol 218:285-93. 2009
  7. pmc Antagonism between binding site affinity and conformational dynamics tunes alternative cis-interactions within Shp2
    Jie Sun
    Department of Molecular and Integrative Physiology, University of Illinois at Urbana Champaign, Urbana, IL 61801, USA
    Nat Commun 4:2037. 2013
  8. pmc N-cadherin regulates spatially polarized signals through distinct p120ctn and β-catenin-dependent signalling pathways
    Mingxing Ouyang
    Department of Bioengineering, University of Illinois, Urbana Champaign, Illinois 61801, USA
    Nat Commun 4:1589. 2013
  9. pmc Rapid activation of Rac GTPase in living cells by force is independent of Src
    Yeh Chuin Poh
    Department of Mechanical Science and Engineering, University of Illinois at Urbana Champaign, Urbana, Illinois, USA
    PLoS ONE 4:e7886. 2009
  10. pmc Quantitative FRET imaging to visualize the invasiveness of live breast cancer cells
    Shaoying Lu
    Department of Bioengineering, University of Illinois at Urbana Champaign, Urbana, Illinois, United States of America
    PLoS ONE 8:e58569. 2013

Collaborators

Detail Information

Publications37

  1. pmc FRET and mechanobiology
    Yingxiao Wang
    Department of Bioengineering, Center for Biophysics and Computational Biology, Institute of Genomic Biology, The Beckman Institute for Advance Science and Technology, University of Illinois at Urbana Champaign, IL 61801, USA
    Integr Biol (Camb) 1:565-73. 2009
    ....
  2. doi request reprint Fluorescence proteins, live-cell imaging, and mechanobiology: seeing is believing
    Yingxiao Wang
    Department of Bioengineering and Beckman Institute for Advanced Science and Technology, University of Illinois, Urbana Champaign, Urbana, IL 61801, USA
    Annu Rev Biomed Eng 10:1-38. 2008
    ..These live-cell imaging technologies, which represent a frontier area in biomedical engineering, can shed new light on the mechanisms regulating mechanobiology at cellular and molecular levels in normal and pathophysiological conditions...
  3. pmc Two distinct phases of calcium signalling under flow
    Bo Liu
    Department of Bioengineering and Beckman Institute for Advanced Science and Technology, University of Illinois, Urbana Champaign, Urbana, IL 61801, USA
    Cardiovasc Res 91:124-33. 2011
    ..Here, we investigate the spatiotemporal pattern of HSS-induced intracellular calcium alteration...
  4. pmc Plectin contributes to mechanical properties of living cells
    Sungsoo Na
    Dept of Mechanical Science, Univ of Illinois, Urbana, IL 61801, USA
    Am J Physiol Cell Physiol 296:C868-77. 2009
    ..Our findings underscore the importance of plectin for mechanical properties, stress propagation, and prestress of living cells, thereby influencing their biological functions...
  5. pmc Visualization of Src activity at different compartments of the plasma membrane by FRET imaging
    Jihye Seong
    Neuroscience Program, Center for Biophysics and Computational Biology, University of Illinois at Urbana Champaign, Urbana, IL 61801, USA
    Chem Biol 16:48-57. 2009
    ..In contrast, the induction of Src activity in nonrafts was faster and stronger, dependent on microtubules. Hence, Src activity is differentially regulated via cytoskeleton at different membrane compartments...
  6. pmc Substrate rigidity regulates Ca2+ oscillation via RhoA pathway in stem cells
    Tae Jin Kim
    Neuroscience Program, Beckman Institute for Advanced Science and Technology, University of Illinois, Urbana Champaign, Urbana, Illinois, USA
    J Cell Physiol 218:285-93. 2009
    ..Therefore, our results demonstrate that RhoA and its downstream molecule ROCK may mediate the substrate rigidity-regulated Ca(2+) oscillation, which determines the physiological functions of HMSCs...
  7. pmc Antagonism between binding site affinity and conformational dynamics tunes alternative cis-interactions within Shp2
    Jie Sun
    Department of Molecular and Integrative Physiology, University of Illinois at Urbana Champaign, Urbana, IL 61801, USA
    Nat Commun 4:2037. 2013
    ..Thus, the antagonistic combination of sequence and position may serve as a basic design principle for proteins with tunable conformations. ..
  8. pmc N-cadherin regulates spatially polarized signals through distinct p120ctn and β-catenin-dependent signalling pathways
    Mingxing Ouyang
    Department of Bioengineering, University of Illinois, Urbana Champaign, Illinois 61801, USA
    Nat Commun 4:1589. 2013
    ..We hence conclude that N-cadherin couples with distinct effectors to polarize phosphoinositide 3-kinase/Rac and myosin II light chain/actin filaments in migrating cells...
  9. pmc Rapid activation of Rac GTPase in living cells by force is independent of Src
    Yeh Chuin Poh
    Department of Mechanical Science and Engineering, University of Illinois at Urbana Champaign, Urbana, Illinois, USA
    PLoS ONE 4:e7886. 2009
    ..These findings suggest that signaling pathways of mechanical forces via integrins might be fundamentally different from those of growth factors...
  10. pmc Quantitative FRET imaging to visualize the invasiveness of live breast cancer cells
    Shaoying Lu
    Department of Bioengineering, University of Illinois at Urbana Champaign, Urbana, Illinois, United States of America
    PLoS ONE 8:e58569. 2013
    ..This readout can be applied to profile the invasiveness of single cells from clinical samples, and to serve as an indicator for screening anti-cancer inhibitors...
  11. pmc Calcium signaling in live cells on elastic gels under mechanical vibration at subcellular levels
    Wagner Shin Nishitani
    Department of Bioengineering and Beckman Institute for Advanced Science and Technology, University of Illinois, Urbana Champaign, Urbana, Illinois, United States of America
    PLoS ONE 6:e26181. 2011
    ..Our newly developed mechanical stimulation device can also provide a powerful tool for the study of molecular mechanism by which cells perceive the mechanical cues at subcellular levels...
  12. pmc Simultaneous visualization of protumorigenic Src and MT1-MMP activities with fluorescence resonance energy transfer
    Mingxing Ouyang
    Department of Bioengineering, Beckman Institute for Advanced Science and Technology, University of Illinois, Urbana Champaign, Illinois 61801, USA
    Cancer Res 70:2204-12. 2010
    ..Our results also showed the potential of mOrange2/mCherry as a new FRET pair, together with the popular variants of CFP and YFP, for the simultaneous visualization of multiple molecular activities in a single live cell...
  13. pmc Rapid signal transduction in living cells is a unique feature of mechanotransduction
    Sungsoo Na
    Departments of Mechanical Science and Engineering and Bioengineering, University of Illinois at Urbana Champaign, Urbana, IL 61801, USA
    Proc Natl Acad Sci U S A 105:6626-31. 2008
    ..These results demonstrate that rapid signal transduction via the prestressed cytoskeleton is a unique feature of mechanotransduction...
  14. pmc Differential RhoA dynamics in migratory and stationary cells measured by FRET and automated image analysis
    John Paul Eichorst
    Center of Biophysics and Computational Biology, University of Illinois at Urbana Champaign, Urbana, IL, USA
    PLoS ONE 3:e4082. 2008
    ....
  15. pmc Determination of hierarchical relationship of Src and Rac at subcellular locations with FRET biosensors
    Mingxing Ouyang
    Department of Bioengineering, Beckman Institute for Advanced Science and Technology, University of Illinois, Urbana Champaign, IL 61801, USA
    Proc Natl Acad Sci U S A 105:14353-8. 2008
    ..In summary, ECFP/YPet may serve as a general FRET pair for the development of highly sensitive biosensors to allow the determination of molecular hierarchies at subcellular locations in live cells...
  16. pmc Integrins regulate VE-cadherin and catenins: dependence of this regulation on Src, but not on Ras
    Yingxiao Wang
    Department of Bioengineering, The Whitaker Institute of Biomedical Engineering, University of California at San Diego, La Jolla, CA 92093, USA
    Proc Natl Acad Sci U S A 103:1774-9. 2006
    ..The results indicate that integrin engagement disrupts VE-cadherin-containing adherens junctions via the activation of Src, but not Ras, possibly as a result of modulation of the actin network...
  17. pmc Live cell imaging of mechanotransduction
    Bo Liu
    Department of Bioengineering and Beckman Institute for Advanced Science and Technology, University of Illinois, Urbana Champaign, Urbana, IL 61801, USA
    J R Soc Interface 7:S365-75. 2010
    ..In summary, FRET technology has provided a powerful tool for the study of mechanotransduction to advance our systematic understanding of the molecular mechanisms by which cells respond to mechanical stimulation...
  18. pmc Visualizing the effect of microenvironment on the spatiotemporal RhoA and Src activities in living cells by FRET
    Tae Jin Kim
    Neuroscience Program University of Illinois at Urbana Champaign, IL 61801, USA
    Small 5:1453-9. 2009
    ..The results help to advance mechanistic understanding of how cells perceive and interpret microenvironments to co-ordinate intracellular molecular signals and ultimately physiological responses...
  19. pmc The spatiotemporal pattern of Src activation at lipid rafts revealed by diffusion-corrected FRET imaging
    Shaoying Lu
    Department of Bioengineering, University of Illinois at Urbana Champaign, Urbana, Illinois, USA
    PLoS Comput Biol 4:e1000127. 2008
    ..Our FE-based method also provides an integrated platform of image analysis for studying molecular mobility and reconstructing the spatiotemporal activation maps of signaling molecules in live cells...
  20. pmc Detection of focal adhesion kinase activation at membrane microdomains by fluorescence resonance energy transfer
    Jihye Seong
    Neuroscience Program, University of Illinois, Urbana Champaign, Urbana, Illinois 61801, USA
    Nat Commun 2:406. 2011
    ..Therefore, FAK is activated at membrane microdomains with distinct activation mechanisms in response to different physiological stimuli...
  21. doi request reprint FRET imaging of calcium signaling in live cells in the microenvironment
    Tongcheng Qian
    Department of Bioengineering and Beckman Institute for Advanced Science and Technology, University of Illinois, Urbana Champaign, IL 61801, USA
    Integr Biol (Camb) 5:431-8. 2013
    ..Therefore, our results shed new light on the molecular mechanism by which HUVECs perceive the microenvironment and regulate intracellular calcium signals...
  22. pmc Phase differential enhancement of FLIM to distinguish FRET components of a biosensor for monitoring molecular activity of Membrane Type 1 Matrix Metalloproteinase in live cells
    John Paul Eichorst
    Center of Biophysics and Computational Biology, University of Illinois at Urbana Champaign, Urbana, IL 61801, USA
    J Fluoresc 21:1763-77. 2011
    ..FLIM carried out in differential enhancement or phase suppression modes, requires only two acquired phase images, and permits rapid imaging of the activity of MT1-MMP in live cells...
  23. pmc Mechanotransduction at focal adhesions: from physiology to cancer development
    Jihye Seong
    Neuroscience Program, University of Illinois, Urbana, IL 61801, USA
    J Cell Mol Med 17:597-604. 2013
    ..We also introduce recent studies on how extracellular matrix and mechanical microenvironments contribute to the development of cancer...
  24. pmc The antagonistic roles of PDGF and integrin αvβ3 in regulating ROS production at focal adhesions
    Li Jung Lin
    Department of Bioengineering, University of Illinois at Urbana Champaign, Urbana, IL 61801, USA
    Biomaterials 34:3807-15. 2013
    ..Therefore, PDGF and integrin αvβ3 coordinate to have an antagonistic effect in the ROS production at FA sites to regulate cell adhesion and migration...
  25. ncbi request reprint Dispersion-relation fluorescence spectroscopy
    Ru Wang
    Quantitative Light Imaging Laboratory, Department of Mechanical Science and Engineering, Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana Champaign, Urbana, Illinois 61801, USA
    Phys Rev Lett 109:188104. 2012
    ..Our data indicate that the fluorescently labeled actin cytoskeleton exhibits active transport motion along a direction parallel to the fibers and diffusive in the perpendicular direction...
  26. pmc Computational analysis of the spatiotemporal coordination of polarized PI3K and Rac1 activities in micro-patterned live cells
    Shaoying Lu
    Department of Bioengineering, University of Illinois at Urbana Champaign, Champaign, Illinois, United States of America
    PLoS ONE 6:e21293. 2011
    ....
  27. pmc Distinct biophysical mechanisms of focal adhesion kinase mechanoactivation by different extracellular matrix proteins
    Jihye Seong
    Neuroscience Program, Department of Mechanical Science and Engineering, and Department of Bioengineering and Beckman Institute for Advanced Science and Technology, University of Illinois, Urbana Champaign, Urbana, IL 61801
    Proc Natl Acad Sci U S A 110:19372-7. 2013
    ....
  28. pmc The effect of differentiation induction on FAK and Src activity in live HMSCs visualized by FRET
    Xiaoling Liao
    Biomaterials and Live Cell Imaging Institute, Chongqing University of Science and Technology, Chongqing, People s Republic of China Beckman Institute for Advanced Science and Technology, Center for Biophysics and Computational Biology, Department of Integrative and Molecular Physiology, Institute for Genomic Biology, University of Illinois, Urbana Champaign, Urbana, Illinois, United States of America
    PLoS ONE 8:e72233. 2013
    ..These results unravel distinct regulation mechanisms of FAK and Src activities during HMSC fate decision, which should advance our understanding of stem cell differentiation in tissue engineering. ..
  29. pmc Fluorescence resonance energy transfer biosensors for cancer detection and evaluation of drug efficacy
    Shaoying Lu
    Department of Bioengineering and Beckman Institute for Advanced Science and Technology, Neuroscience Program, Center of Biophysics and Computational Biology, Institute of Genomic Biology, University of Illinois at Urbana Champaign, 405 N Mathews Avenue, Urbana, IL 61801, USA
    Clin Cancer Res 16:3822-4. 2010
    ..This biosensor allowed the detection of cancerous and drug-resistant cells, and the evaluation of kinase inhibitor efficacy. Future biosensor development and imaging can increasingly contribute to cancer diagnosis and therapeutics...
  30. ncbi request reprint Analysis of integrin signaling by fluorescence resonance energy transfer
    Yingxiao Wang
    Department of Bioengineering and Molecular and Integrative Physiology, Neuroscience Program, Center for Biophysics and Computational Biology, Beckman Institute for Advanced Science and Technology, University of Illinois, Urbana Champaign, USA
    Methods Enzymol 426:177-201. 2007
    ..The dynamic and subcellular visualization enabled by FRET can shed new light on the molecular mechanisms regulating integrin signaling and advance our knowledge in the understanding of integrin-related pathophysiological processes...
  31. doi request reprint Genetically encoded fluorescent biosensors for live-cell imaging of MT1-MMP protease activity
    Mingxing Ouyang
    Department of Bioengineering, University of Illinois at Urbana Champaign, Champaign, IL, USA
    Methods Mol Biol 1071:163-74. 2014
    ..We also provide brief guidelines for observing MT1-MMP subcellular activity by fluorescence resonance energy transfer (FRET) in a cell migration assay. ..
  32. pmc Visualization of polarized membrane type 1 matrix metalloproteinase activity in live cells by fluorescence resonance energy transfer imaging
    Mingxing Ouyang
    Department of Bioengineering and the Beckman Institute for Advanced Science and Technology, University of Illinois, Urbana Champaign, Illinois 61801, USA
    J Biol Chem 283:17740-8. 2008
    ..Hence, the MT1-MMP biosensor provides a powerful tool for characterizing the molecular processes underlying the spatiotemporal regulation of this critical class of enzymes...
  33. ncbi request reprint Effects of flow patterns on the localization and expression of VE-cadherin at vascular endothelial cell junctions: in vivo and in vitro investigations
    Hui Miao
    Department of Bioengineering, The Whitaker Institute of Biomedical Engineering, University of California, San Diego, La Jolla, CA 92093 0412, USA
    J Vasc Res 42:77-89. 2005
    ....
  34. ncbi request reprint Shear stress and VEGF activate IKK via the Flk-1/Cbl/Akt signaling pathway
    Yingxiao Wang
    Department of Bioengineering, The Whitaker Institute of Biomedical Engineering, University of California San Diego, La Jolla 92093 0427, USA
    Am J Physiol Heart Circ Physiol 286:H685-92. 2004
    ..Therefore, our results indicate that the signaling events induced by shear stress and VEGF converge at the membrane receptor Flk-1 and that these stimuli share the Flk-1/Cbl/Akt pathway in activating IKK activation...
  35. pmc Micro/nano-fabrication technologies for cell biology
    Tongcheng Qian
    Department of Bioengineering, University of Illinois at Urbana Champaign, Urbana, IL 61801, USA
    Med Biol Eng Comput 48:1023-32. 2010
    ..Finally, potential schemes in cell biology involving micro/nano-fabrication technologies are proposed to provide perspectives on the future research activities...
  36. ncbi request reprint Visualizing the mechanical activation of Src
    Yingxiao Wang
    Department of Bioengineering and The Whitaker Institute of Biomedical Engineering, La Jolla, California 92093, USA
    Nature 434:1040-5. 2005
    ..We find that the transmission of mechanically induced Src activation is a dynamic process that directs signals via the cytoskeleton to spatial destinations...
  37. ncbi request reprint Laser tweezers in the study of mechanobiology in live cells
    Elliot L Botvinick
    Department of Biomedical Engineering, Beckman Laser Institute, University of California, Irvine, California 92612, USA
    Methods Cell Biol 82:497-523. 2007
    ..Methods of analyzing the distribution of activated Src molecules are detailed with examples of cells with varying degrees of mechanostimulation...

Research Grants1

  1. FRET Imaging of Molecular Hierarchy at Subcellular Levels in Mechanotransduction
    Yingxiao Wang; Fiscal Year: 2010
    ..Therefore, the success of the proposed project will have significant impact on improving public health. ..