Xuemin Wang

..The method provides quantification of most major plant hormones in a single run from 50 mg of fresh plant tissue. Extraction and quantitative analysis of 40 samples takes 2-3 d...

..The regulatory processes in which PA has been implicated include signaling pathways in cell growth, proliferation, reproduction, and responses to hormones and biotic and abiotic stresses...

..The lipid-signaling process may play a role in connecting membrane sensing of nutrient status to increased plant growth and biomass production...

..PA is involved in the activation of SPHK, and activation of PLDα1 requires SPHK activity. The data suggest that SPHK/phyto-S1P and PLDα1A are co-dependent in amplification of response to ABA, mediating stomatal closure in Arabidopsis...

..These data suggest that alteration of pPLAIIIβ expression and the resulting lipid changes alter cellulose content and cell elongation in Arabidopsis...

..These data suggest that PLDalpha3 positively mediates plant responses to hyperosmotic stresses and that increased PLDalpha3 expression and associated lipid changes promote root growth, flowering, and stress avoidance...

..These data suggest that NPC4-produced DAG is converted to PA and that NPC4 and its derived lipids positively modulate ABA response and promote plant tolerance to drought and salt stresses...

..The results indicate that the H(2)O(2)-promoted interaction of GAPC and PLDδ may provide a direct connection between membrane lipid-based signaling, energy metabolism and growth control in the plant response to ROS and water stress...

..The study shows that AtPLAI is an acyl hydrolase, rather than a specific phospholipase A. AtPLAI is involved in basal JA production and Arabidopsis resistance to the necrotrophic fungus B. cinerea...

..A high level of PLDalpha1 is detrimental to seed quality, and attenuation of PLDalpha1 expression has the potential to improve oil stability, seed quality and seed longevity...

..It promotes stomatal closure at earlier stages, but disrupts membranes in prolonged drought stress. These findings are discussed in relation to the understanding of PLD functions and potential applications...

..These differences provide insights into determining the cellular function of specific phospholipases in plants, and they can be explored for different industrial applications...

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..The PA-SPHK interaction depends on the PA molecular species. The data suggest that these two Arabidopsis SPHKs are molecular targets of PA, and the PA stimulation of SPHK is part of the signaling networks in Arabidopsis...

..The results suggest that hydrolysis of phosphatidylcholine by PLD zetas during phosphorus starvation contributes to the supply of inorganic phosphorus for cell metabolism and diacylglycerol moieties for galactolipid synthesis...

..These differences in biochemical and molecular properties suggest that the highly homologous PLDgammas are subjected to unique regulations and might have distinguishable functions...

..Structurally diverse compounds from complicated biological matrices are determined with high selectivity and sensitivity...

..The capacity to simultaneously quantify multiple classes of phytohormones will facilitate the study of hormone function and cross-talk...

..The results reveal a bifurcating signaling pathway that regulates plant water loss...

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..We successfully engineered an entire plant natural product pathway into a mammalian host...

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..PLDs and their product phosphatidic acid are involved in various stress responses, including water deficits, salts, wounding, and elicitation. Increasing evidence supports a role of PLD in the abscisic acid signaling cascades...

..Phospholipase D enzymes and phosphatidic acid may play a broader role in lipid signaling in plants than in other systems...

..These results demonstrate that activation of oleate-stimulated PLDdelta constitutes an important step in the plant response to H2O2 and increasing plant stress tolerance...

..These findings identify a new way in which amino acids regulate a key step in translation initiation and indicate that this involves a novel amino acid-sensitive signaling mechanism...

..These results suggest that PLDalpha1 plays a key role in freezing tolerance of Arabidopsis by modulating the cold-responsive genes and accumulation of osmolytes...

..mTOR plays key roles in cell physiology. mTOR regulates numerous components involved in protein synthesis, including initiation and elongation factors, and the biogenesis of ribosomes themselves...

..This indicates that Ca(2+) activates PLD by promoting the binding of phospholipid substrate to the catalytic site of the enzyme...

..These data suggest a negative role for PLDalpha1 and a positive role for PLDdelta in freezing tolerance...

..Such lipidomic analyses have been used to uncover roles for lipids in plant response to stresses and to identify in vivo functions of genes involved in lipid metabolism...

..In addition, we provide guidelines on using small molecule inhibitors to assess the involvement of specific signaling pathways in controlling translation factors and protein synthesis...

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..This combined information on structural features, expression profiles, and cellular localization will assist the basis for dissection of PLD isoform function in rice...

..The RAIP motif thus promotes the mTOR-dependent phosphorylation of multiple sites in 4E-BP1 independently of the 4E-BP1/raptor interaction...

..Our studies reveal a nuclear pathway by which neurotoxin/Cdk5 induces neuronal apoptosis through inhibiting prosurvival nuclear machinery...

..Thus, physical and functional interactions between PLD and actin in PLD family members from bacteria to mammals are highly conserved throughout evolution...

..However, in 4E-BP3, replacement of the alanine at the position corresponding to S112 by serine or glutamate did not confer the ability to be released from eIF4E in response to insulin...

..These data are consistent with a model in which the physical state of the actin cytoskeleton is a critical determinant of its regulation of PLD activity...

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..Our data reveal that these stress-inducing agents, which are widely used to study stress-signalling in mammalian cells, exert multiple and complex inhibitory effects on the translational machinery...

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..Together, these data indicate that calpain-p35-p25/cdk5-mediated inactivation of MEF2 plays a critical role in dopaminergic loss in vivo...

..These results indicate that PLDzeta1 and PLDzeta2 play a role in regulating root development in response to nutrient limitation...

..PLD and PA play multiple roles in the signaling networks of plant response to abscisic acid and reactive oxygen species...

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..These data have important implications for understanding signaling downstream of mTOR and the development of new strategies to impair mTOR signaling...

..This study identifies the transcription factor MEF2 as a target of cAMP-PKA pathway and demonstrates that PKA phosphorylation of MEF2 is a key step in modulating its DNA binding activity and ability to promote neuronal survival...

..These results demonstrate the contrasting and complementary effects of the Ca(2+)- and lipid-binding properties of the C2 and catalytic domains of plant PLD and provide insight into the mechanism by which Ca(2+) regulates PLD activity...

..Our studies give further supports for JNK-targeting strategy in preventing neuronal apoptosis...

..These signaling mechanisms may mediate the growth-promoting effect of ANG II in human cSMCs...

..The identification of ABI1 as a direct target of the lipid messenger PA provides a functional link between the two families of important signaling enzymes, PLD and PP2C...

..The results taken together allow us to describe an anchorage-scooting model for the synergistic activation of PLDbeta by PI(4,5)P2 and Ca2+...