Research Topics
| James Hc WangSummaryAffiliation: University of Pittsburgh Country: USA Publications
Research Grants
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Detail Information
Publications
Tendon biomechanics and mechanobiology--a minireview of basic concepts and recent advancementsJames H C Wang
MechanoBiology Laboratory, Department of Orthopaedic Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA
J Hand Ther 25:133-40; quiz 141. 2012..Moreover, along with scaffolds that can mimic tendon matrix environments and platelet-rich plasma, which serves as a source of growth factors, TSCs may be the optimal cell type for enhancing repair of injured tendons...- Characterization of differential properties of rabbit tendon stem cells and tenocytesJianying Zhang
MechanoBiology Laboratory, Departments of Orthopaedic Surgery, Bioengineering, and Mechanical Engineering and Materials Science, University of Pittsburgh, Pittsburgh, PA 15213, USA
BMC Musculoskelet Disord 11:10. 2010..However, the differential properties of TSCs and tenocytes remain largely undefined. This study aims to characterize the properties of these tendon cells derived from rabbits... - Differential properties of human ACL and MCL stem cells may be responsible for their differential healing capacityJianying Zhang
MechanoBiology Laboratory, Department of Orthopedic Surgery, University of Pittsburgh, PA 15213, USA
BMC Med 9:68. 2011.... - Mechanoregulation of gene expression in fibroblastsJames H C Wang
MechanoBiology Laboratory, Department of Orthopaedic Surgery, University of Pittsburgh, 210 Lothrop St, BST, E1640, Pittsburgh, PA 15213, USA
Gene 391:1-15. 2007..Future research directions in mechanoregulation of gene expression are also suggested... - Controlling cell responses to cyclic mechanical stretchingJames H C Wang
MechanoBiology Laboratory, Departments of Orthopaedic Surgery and Bioengineering, University of Pittsburgh, Pittsburgh, PA 15213, USA
Ann Biomed Eng 33:337-42. 2005..We suggest that microgrooved silicone substrates can be used to study biological responses of tendon or ligament fibroblasts to repetitive mechanical stretching conditions in a more controlled manner... - Biomechanical basis for tendinopathyJames H C Wang
MechanoBiology Laboratory, Department of Orthopaedic Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA
Clin Orthop Relat Res 443:320-32. 2006..Research is required to better understand the mechanisms of tendinopathy at the tissue, cellular, and molecular levels and to develop new scientifically based modalities to treat tendinopathy more effectively... - An introductory review of cell mechanobiologyJ H C Wang
MechanoBiology Laboratory, Department of Orthopaedic Surgery, University of Pittsburgh, 210 Lothrop St BST, E1640, Pittsburgh, PA 15213, USA
Biomech Model Mechanobiol 5:1-16. 2006.... - Cell traction force and measurement methodsJames H C Wang
Departments of Orthopaedic Surgery, Bioengineering and Mechanical Engineering, MechanoBiology Laboratory, E1640 Biomedical Science Tower, 210 Lothrop Street, Pittsburgh, PA 15213, USA
Biomech Model Mechanobiol 6:361-71. 2007.... - Fibroblast responses to cyclic mechanical stretching depend on cell orientation to the stretching directionJames H C Wang
Department of Orthopaedic Surgery, University of Pittsburgh, BST, E1641, 210 Lothrop Street, P O Box 71199, Pittsburgh, PA 15213, USA
J Biomech 37:573-6. 2004..Thus, these results show that fibroblast responses to mechanical stretching depend on cell orientation to the stretching direction... - Cyclic mechanical stretching of human tendon fibroblasts increases the production of prostaglandin E2 and levels of cyclooxygenase expression: a novel in vitro model studyJames H C Wang
Musculoskeletal Research Center, Department of Orthopaedic Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA
Connect Tissue Res 44:128-33. 2003..As PGE(2) is a known inflammatory mediator of tendinitis, the contribution of COX-1 and COX-2 to PGE(2) production and their roles in tendon inflammation are clearly indicated... - Cell orientation determines the alignment of cell-produced collagenous matrixJames H C Wang
Department of Orthopaedic Surgery, Musculoskeletal Research Center, University of Pittsburgh Medical Center, E1641 Biomedical Science Tower, 210 Lothrop Street, PO Box 71199, Pittsburgh, PA 15213, USA
J Biomech 36:97-102. 2003..This may enhance the mechanical properties of healing ligaments and tendons... - Specificity of endothelial cell reorientation in response to cyclic mechanical stretchingJ H Wang
Department of Biomedical Engineering, Washington University, St Louis, MO 63130, USA
J Biomech 34:1563-72. 2001..Finally, abolishing microtubules prevented neither the formation of stress fibers nor cell reorientation. Thus, endothelial cells respond very specifically to the type of deformation imposed upon them... - Production of PGE(2) increases in tendons subjected to repetitive mechanical loading and induces differentiation of tendon stem cells into non-tenocytesJianying Zhang
MechanoBiology Laboratory, Department of Orthopaedic Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA
J Orthop Res 28:198-203. 2010.... - Leukotrienes and tyrosine phosphorylation mediate stretching-induced actin cytoskeletal remodeling in endothelial cellsJ H Wang
Department of Biomedical Engineering, Washington University, St Louis, Missouri, USA
Cell Motil Cytoskeleton 46:137-45. 2000..Finally, leukotrienes and tyrosine phosphorylation are necessary for actin cytoskeletal remodeling of the endothelial cells in response to mechanical stretching... - Mechanics rules cell biologyJames Hc Wang
MechanoBiology Laboratory, Department of Orthopaedic Surgery, University of Pittsburgh School of Medicine, 210 Lothrop St, BST E1640, Pittsburgh, PA 15213, USA
Sports Med Arthrosc Rehabil Ther Technol 2:16. 2010.... - Alignment and proliferation of MC3T3-E1 osteoblasts in microgrooved silicone substrata subjected to cyclic stretchingJ H Wang
Noyes Giannestras Biomechanics Laboratory, Department of Aerospace Engineering and Engineering Mechanics, College of Engineering, University of Cincinnati, Cincinnati, OH 45221 0048, USA
J Biomech 33:729-35. 2000..The silicone microgrooves can also be useful for delivering defined substrate strains to other adherent cells in cultures... - Mechanobiology of tendonJames H C Wang
MechanoBiology Laboratory, Department of Orthopaedic Surgery, University of Pittsburgh, 210 Lothrop St, BST, E1647, Pittsburgh, PA 15213, USA
J Biomech 39:1563-82. 2006..Finally, future research directions in tendon mechanobiology research are discussed... - Contractility affects stress fiber remodeling and reorientation of endothelial cells subjected to cyclic mechanical stretchingJ H Wang
Department of Biomedical Engineering, Washington University, Sr Louis, MO, USA
Ann Biomed Eng 28:1165-71. 2000..We suggest that the effect of ROS on stress fiber remodeling and cell reorientation is due to the ability of ROS to regulate cellular contractility, which is crucial for these cellular responses... - Substrate deformation determines actin cytoskeleton reorganization: A mathematical modeling and experimental studyJ H Wang
Department of Aerospace Engineering and Engineering Mechanics, College of Engineering, Cincinnati, OH, 45221 0071, USA
J Theor Biol 202:33-41. 2000..The prediction was confirmed by experiments conducted on both fibroblasts and endothelial cells. The present model may be relevant for understanding better the effects of mechanical stimuli on the cells... - A new method for the representation of articular surfaces using the influence surface theory of platesJ H Wang
Orthopaedic Biomechanics Lab, Department of Orthopaedic Surgery, Texas Tech University Health Sciences Center, El Paso, TX 79905, USA
J Biomech 33:629-33. 2000..The effectiveness of the present method was shown by reconstruction of a human femoral surface and a mathematical cone... - The strain magnitude and contact guidance determine orientation response of fibroblasts to cyclic substrate strainsJ H Wang
Department of Aerospace Engineering and Engineering Mechanics, College of Engineering, University of Cincinnati, OH 45221 0048, USA
Connect Tissue Res 41:29-36. 2000..e., strain-direction independent). It also suggested that the failure of fibroblasts to change orientations in vivo may result from the contact guidance provided by collagen fibers... - Platelet-rich plasma releasate promotes differentiation of tendon stem cells into active tenocytesJianying Zhang
Department of Orthopaedic Surgery, University of Pittsburgh, PA 15213, USA
Am J Sports Med 38:2477-86. 2010..The aim of this study was to determine the effects of PRP, in the form of PRP-clot releasate (PRCR), on tendon stem cells (TSCs), a newly discovered cell population in tendons... - Intra- and inter-observer reproducibility of volume measurement of knee cartilage segmented from the OAI MR image set using a novel semi-automated segmentation methodK T Bae
Department of Radiology, University of Pittsburgh, Pittsburgh, PA 15213, USA
Osteoarthritis Cartilage 17:1589-97. 2009.... - The use of porcine small intestinal submucosa to enhance the healing of the medial collateral ligament--a functional tissue engineering study in rabbitsVolker Musahl
Musculoskeletal Research Center, Department of Orthopedic Surgery, University of Pittsburgh, E1641 Biomedical Science Tower, 210 Lothrop Street, P.O. Box 71199, Pittsburgh, PA 15213, USA
J Orthop Res 22:214-20. 2004.... - Monitoring cell adhesion by using thickness shear mode acoustic wave sensorsFang Li
Department of Mechanical Engineering and Material Science, University of Pittsburgh, Pittsburgh, PA 15261, United States
Biosens Bioelectron 23:42-50. 2007..The results indicate that resistance changes are related to the disruption of actin cytoskeleton and dosage-dependent... - Cell shape regulates collagen type I expression in human tendon fibroblastsFang Li
Department of Mechanical Engineering and Materials Science, University of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA
Cell Motil Cytoskeleton 65:332-41. 2008.... - Leukotriene B4 at low dosage negates the catabolic effect of prostaglandin E2 in human patellar tendon fibroblastsBhavani P Thampatty
MechanoBiology Laboratory, Department of Orthopaedic Surgery, University of Pittsburgh, E1641 Biomedical Science Tower 210 Lothrop Street, Pittsburgh, PA 15213, USA
Gene 372:103-9. 2006..Thus, our findings suggest that although LTB4 is generally thought to be pathogenic, low levels of LTB4 are actually beneficial in maintaining tendon tissue homeostasis... - A multi-station culture force monitor system to study cellular contractilityBrian H Campbell
Musculoskeletal Research Center, Department of Orthopaedic Surgery, University of Pittsburgh, Pittsburgh, PA, USA
J Biomech 36:137-40. 2003..Therefore, the system can facilitate statistical design and analysis of experiments to study the effects of growth factors (e.g., TGF-betas) on cellular contraction and their potential role in scar tissue formation... - A multi-station dynamic-culture force monitor system to study cell mechanobiologyKatherin A Peperzak
MechanoBiology Laboratory, Musculoskeletal Research Center, Department of Orthopaedic Surgery, University of Pittsburgh Medical Center, E1641 Biomedical Science Tower, 210 Lothrop Street, PO Box 71199, Pittsburgh, PA 15213, USA
Med Eng Phys 26:355-8. 2004..This new system will facilitate the study of the effects of dynamic mechanical loading on cells, remodeling of extracellular matrix, and cell-matrix interactions in vitro... - Alpha-smooth muscle actin expression enhances cell traction forceJianxin Chen
Department of Orthopaedic Surgery, MechanoBiology Laboratory, University of Pittsburgh, Pennsylvania 15213, USA
Cell Motil Cytoskeleton 64:248-57. 2007..Finally, CTFs were found to vary among a population of myofibroblasts, suggesting that alpha-SMA protein expression levels of individual cells also vary... - EP4 receptor regulates collagen type-I, MMP-1, and MMP-3 gene expression in human tendon fibroblasts in response to IL-1 beta treatmentBhavani P Thampatty
MechanoBiology Laboratory, Departments of Orthopaedic Surgery and Bioengineering, University of Pittsburgh, E1640 Biomedical Science Tower, 210 Lothrop Street, Pittsburgh, PA 15213, USA
Gene 386:154-61. 2007.... - Repetitive mechanical stretching modulates IL-1beta induced COX-2, MMP-1 expression, and PGE2 production in human patellar tendon fibroblastsGuoguang Yang
MechanoBiology Laboratory, Department of Orthopaedic Surgery, University of Pittsburgh, E1641 Biomedical Science Tower, 210 Lothrop Street, Pittsburgh, PA 15213, USA
Gene 363:166-72. 2005..Thus, the results suggest that repetitive, small-magnitude stretching is anti-inflammatory, whereas large-magnitude stretching is pro-inflammatory. Therefore, moderate exercise may be beneficial to reducing tendon inflammation... - Proliferation and collagen production of human patellar tendon fibroblasts in response to cyclic uniaxial stretching in serum-free conditionsGuoguang Yang
Musculoskeletal Research Center, Departments of Orthopaedic Surgery and Bioengineering, University of Pittsburgh Medical Center, E1641 Biomedical Science Tower, 210 Lothrop Street, PO Box 71199, Pittsburgh, PA 15213, USA
J Biomech 37:1543-50. 2004..The results suggest that mechanical stretching can modulate proliferation of human tendon fibroblasts in the absence of serum and increase the cellular production of collagen type I, which is at least in part mediated by TGF-beta1... - Prostaglandin E2 affects proliferation and collagen synthesis by human patellar tendon fibroblastsFeridun Cilli
MechanoBiology Laboratory, Musculoskeletal Research Center, Department of Orthopaedic Surgery, University of Pittsburgh Medical Center, Pittsburgh, PA 15213, USA
Clin J Sport Med 14:232-6. 2004..CLINICAL RELEVANCE: Based on these in vitro findings, we speculate that production of prostaglandin E2 in tendons might play some role in the acellularity and matrix disorganization seen in exercise-induced tendinopathy... - Healing and normal fibroblasts exhibit differential proliferation, collagen production, alpha-SMA expression, and contractionCharu Agarwal
Department of Orthopaedic Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
Ann Biomed Eng 34:653-9. 2006..Taken together, the results of this study show that healing fibroblasts possess a differential proliferation, alpha-SMA protein expression, and contraction than normal fibroblasts... - Determining substrate displacement and cell traction fields--a new approachZhaochun Yang
MechanoBiology Laboratory, Departments of Orthopaedic Surgery, Bioengineering and Mechanical Engineering, E1640 Biomedical Science Tower, 210 Lothrop Street, Pittsburgh, PA 15213, USA
J Theor Biol 242:607-16. 2006.... - RGD peptide-conjugated poly(dimethylsiloxane) promotes adhesion, proliferation, and collagen secretion of human fibroblastsBin Li
MechanoBiology Laboratory, Department of Orthopaedic Surgery, University of Pittsburgh, Pennsylvania 15213, USA
J Biomed Mater Res A 79:989-98. 2006..Finally, the RGD-conjugated PDMS surfaces are resistant to autoclaving and UV irradiation, which enables them to be repeatedly used in cell culture studies... - A new approach to study fibroblast migrationBhavani P Thampatty
MechanoBiology Laboratory, Departments of Orthopaedic Surgery, Bioengineering, and Mechanical Engineering, University of Pittsburgh, 210 Lothrop Street, Pittsburgh, PA 15213, USA
Cell Motil Cytoskeleton 64:1-5. 2007.... - Decreasing inflammatory response of injured patellar tendons results in increased collagen fibril diametersDimosthenis A Alaseirlis
Department of Orthopaedic Surgery, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA
Connect Tissue Res 46:12-7. 2005..Therefore, these results suggest that decreasing the inflammatory response in the early stages of tendon wound healing enhances the quality of the healing tendon through increased collagen fiber diameter and better organization... - Vanadate ingestion enhances the organization and collagen fibril diameters of rat healing medical collateral ligamentsJianxin Chen
MechanoBiology Laboratory, Department of Orthopaedic Surgery, University of Pittsburgh School of Medicine, 210 Lothrop Street, Biomedical Science Tower, E1641, Pittsburgh, PA 15213, USA
Knee Surg Sports Traumatol Arthrosc 14:750-5. 2006..001). These results indicate that application of vanadate may be a promising tissue engineering approach to enhance the quality of healing tissues such as injured MCLs... - Development of micropost force sensor array with culture experiments for determination of cell traction forcesBin Li
MechanoBiology Laboratory, Department of Orthopaedic Surgery, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA
Cell Motil Cytoskeleton 64:509-18. 2007..5 nN. Culture experiments with various types of cells showed that this MFSA technology can effectively determine CTFs of cells with different sizes and traction force magnitudes... - Repeated exposure of tendon to prostaglandin-E2 leads to localized tendon degenerationMustafa H Khan
Musculoskeletal Research Center, Department of Orthopaedic Surgery, University of Pittsburgh Medical Center, 210 Lothrop Street, Pittsburgh, PA 15213, USA
Clin J Sport Med 15:27-33. 2005..Therefore, PGE(2) produced by tendon fibroblasts in response to repetitive mechanical loading in vivo might contribute to the development of exercise-induced tendinopathy...
Research Grants
- An interdisciplinary and integrative study of tendinopathyJames H C Wang; Fiscal Year: 2010....
- An interdisciplinary and integrative study of tendinopathyJames Wang; Fiscal Year: 2009....
- A Request for support of 25th SPRSM MeetingJames Wang; Fiscal Year: 2006....
- A Multidisciplinary Study of Mechanisms for TendinitisJames Wang; Fiscal Year: 2005..The findings from this study will also be useful in helping design experiments to study other repetitive motion disorders (e.g., carpal tunnel syndrome). ..
- An interdisciplinary and integrative study of tendinopathyJames Wang; Fiscal Year: 2009....