James Hc Wang

Summary

Affiliation: University of Pittsburgh
Country: USA

Publications

  1. pmc Tendon biomechanics and mechanobiology--a minireview of basic concepts and recent advancements
    James H C Wang
    MechanoBiology Laboratory, Department of Orthopaedic Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA
    J Hand Ther 25:133-40; quiz 141. 2012
  2. pmc Characterization of differential properties of rabbit tendon stem cells and tenocytes
    Jianying Zhang
    MechanoBiology Laboratory, Departments of Orthopaedic Surgery, Bioengineering, and Mechanical Engineering and Materials Science, University of Pittsburgh, Pittsburgh, PA 15213, USA
    BMC Musculoskelet Disord 11:10. 2010
  3. pmc Differential properties of human ACL and MCL stem cells may be responsible for their differential healing capacity
    Jianying Zhang
    MechanoBiology Laboratory, Department of Orthopedic Surgery, University of Pittsburgh, PA 15213, USA
    BMC Med 9:68. 2011
  4. pmc Mechanoregulation of gene expression in fibroblasts
    James H C Wang
    MechanoBiology Laboratory, Department of Orthopaedic Surgery, University of Pittsburgh, 210 Lothrop St, BST, E1640, Pittsburgh, PA 15213, USA
    Gene 391:1-15. 2007
  5. ncbi request reprint Controlling cell responses to cyclic mechanical stretching
    James H C Wang
    MechanoBiology Laboratory, Departments of Orthopaedic Surgery and Bioengineering, University of Pittsburgh, Pittsburgh, PA 15213, USA
    Ann Biomed Eng 33:337-42. 2005
  6. ncbi request reprint Biomechanical basis for tendinopathy
    James H C Wang
    MechanoBiology Laboratory, Department of Orthopaedic Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA
    Clin Orthop Relat Res 443:320-32. 2006
  7. ncbi request reprint An introductory review of cell mechanobiology
    J H C Wang
    MechanoBiology Laboratory, Department of Orthopaedic Surgery, University of Pittsburgh, 210 Lothrop St BST, E1640, Pittsburgh, PA 15213, USA
    Biomech Model Mechanobiol 5:1-16. 2006
  8. ncbi request reprint Cell traction force and measurement methods
    James H C Wang
    Departments of Orthopaedic Surgery, Bioengineering and Mechanical Engineering, MechanoBiology Laboratory, E1640 Biomedical Science Tower, 210 Lothrop Street, Pittsburgh, PA 15213, USA
    Biomech Model Mechanobiol 6:361-71. 2007
  9. ncbi request reprint Fibroblast responses to cyclic mechanical stretching depend on cell orientation to the stretching direction
    James H C Wang
    Department of Orthopaedic Surgery, University of Pittsburgh, BST, E1641, 210 Lothrop Street, P O Box 71199, Pittsburgh, PA 15213, USA
    J Biomech 37:573-6. 2004
  10. ncbi request reprint Cyclic mechanical stretching of human tendon fibroblasts increases the production of prostaglandin E2 and levels of cyclooxygenase expression: a novel in vitro model study
    James H C Wang
    Musculoskeletal Research Center, Department of Orthopaedic Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA
    Connect Tissue Res 44:128-33. 2003

Collaborators

Detail Information

Publications42

  1. pmc Tendon biomechanics and mechanobiology--a minireview of basic concepts and recent advancements
    James H C Wang
    MechanoBiology Laboratory, Department of Orthopaedic Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA
    J Hand Ther 25:133-40; quiz 141. 2012
    ..Moreover, along with scaffolds that can mimic tendon matrix environments and platelet-rich plasma, which serves as a source of growth factors, TSCs may be the optimal cell type for enhancing repair of injured tendons...
  2. pmc Characterization of differential properties of rabbit tendon stem cells and tenocytes
    Jianying Zhang
    MechanoBiology Laboratory, Departments of Orthopaedic Surgery, Bioengineering, and Mechanical Engineering and Materials Science, University of Pittsburgh, Pittsburgh, PA 15213, USA
    BMC Musculoskelet Disord 11:10. 2010
    ..However, the differential properties of TSCs and tenocytes remain largely undefined. This study aims to characterize the properties of these tendon cells derived from rabbits...
  3. pmc Differential properties of human ACL and MCL stem cells may be responsible for their differential healing capacity
    Jianying Zhang
    MechanoBiology Laboratory, Department of Orthopedic Surgery, University of Pittsburgh, PA 15213, USA
    BMC Med 9:68. 2011
    ....
  4. pmc Mechanoregulation of gene expression in fibroblasts
    James H C Wang
    MechanoBiology Laboratory, Department of Orthopaedic Surgery, University of Pittsburgh, 210 Lothrop St, BST, E1640, Pittsburgh, PA 15213, USA
    Gene 391:1-15. 2007
    ..Future research directions in mechanoregulation of gene expression are also suggested...
  5. ncbi request reprint Controlling cell responses to cyclic mechanical stretching
    James H C Wang
    MechanoBiology Laboratory, Departments of Orthopaedic Surgery and Bioengineering, University of Pittsburgh, Pittsburgh, PA 15213, USA
    Ann Biomed Eng 33:337-42. 2005
    ..We suggest that microgrooved silicone substrates can be used to study biological responses of tendon or ligament fibroblasts to repetitive mechanical stretching conditions in a more controlled manner...
  6. ncbi request reprint Biomechanical basis for tendinopathy
    James H C Wang
    MechanoBiology Laboratory, Department of Orthopaedic Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA
    Clin Orthop Relat Res 443:320-32. 2006
    ..Research is required to better understand the mechanisms of tendinopathy at the tissue, cellular, and molecular levels and to develop new scientifically based modalities to treat tendinopathy more effectively...
  7. ncbi request reprint An introductory review of cell mechanobiology
    J H C Wang
    MechanoBiology Laboratory, Department of Orthopaedic Surgery, University of Pittsburgh, 210 Lothrop St BST, E1640, Pittsburgh, PA 15213, USA
    Biomech Model Mechanobiol 5:1-16. 2006
    ....
  8. ncbi request reprint Cell traction force and measurement methods
    James H C Wang
    Departments of Orthopaedic Surgery, Bioengineering and Mechanical Engineering, MechanoBiology Laboratory, E1640 Biomedical Science Tower, 210 Lothrop Street, Pittsburgh, PA 15213, USA
    Biomech Model Mechanobiol 6:361-71. 2007
    ....
  9. ncbi request reprint Fibroblast responses to cyclic mechanical stretching depend on cell orientation to the stretching direction
    James H C Wang
    Department of Orthopaedic Surgery, University of Pittsburgh, BST, E1641, 210 Lothrop Street, P O Box 71199, Pittsburgh, PA 15213, USA
    J Biomech 37:573-6. 2004
    ..Thus, these results show that fibroblast responses to mechanical stretching depend on cell orientation to the stretching direction...
  10. ncbi request reprint Cyclic mechanical stretching of human tendon fibroblasts increases the production of prostaglandin E2 and levels of cyclooxygenase expression: a novel in vitro model study
    James H C Wang
    Musculoskeletal Research Center, Department of Orthopaedic Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA
    Connect Tissue Res 44:128-33. 2003
    ..As PGE(2) is a known inflammatory mediator of tendinitis, the contribution of COX-1 and COX-2 to PGE(2) production and their roles in tendon inflammation are clearly indicated...
  11. ncbi request reprint Cell orientation determines the alignment of cell-produced collagenous matrix
    James H C Wang
    Department of Orthopaedic Surgery, Musculoskeletal Research Center, University of Pittsburgh Medical Center, E1641 Biomedical Science Tower, 210 Lothrop Street, PO Box 71199, Pittsburgh, PA 15213, USA
    J Biomech 36:97-102. 2003
    ..This may enhance the mechanical properties of healing ligaments and tendons...
  12. ncbi request reprint Specificity of endothelial cell reorientation in response to cyclic mechanical stretching
    J H Wang
    Department of Biomedical Engineering, Washington University, St Louis, MO 63130, USA
    J Biomech 34:1563-72. 2001
    ..Finally, abolishing microtubules prevented neither the formation of stress fibers nor cell reorientation. Thus, endothelial cells respond very specifically to the type of deformation imposed upon them...
  13. doi request reprint Production of PGE(2) increases in tendons subjected to repetitive mechanical loading and induces differentiation of tendon stem cells into non-tenocytes
    Jianying Zhang
    MechanoBiology Laboratory, Department of Orthopaedic Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA
    J Orthop Res 28:198-203. 2010
    ....
  14. ncbi request reprint Leukotrienes and tyrosine phosphorylation mediate stretching-induced actin cytoskeletal remodeling in endothelial cells
    J H Wang
    Department of Biomedical Engineering, Washington University, St Louis, Missouri, USA
    Cell Motil Cytoskeleton 46:137-45. 2000
    ..Finally, leukotrienes and tyrosine phosphorylation are necessary for actin cytoskeletal remodeling of the endothelial cells in response to mechanical stretching...
  15. pmc Mechanics rules cell biology
    James Hc Wang
    MechanoBiology Laboratory, Department of Orthopaedic Surgery, University of Pittsburgh School of Medicine, 210 Lothrop St, BST E1640, Pittsburgh, PA 15213, USA
    Sports Med Arthrosc Rehabil Ther Technol 2:16. 2010
    ....
  16. ncbi request reprint Alignment and proliferation of MC3T3-E1 osteoblasts in microgrooved silicone substrata subjected to cyclic stretching
    J H Wang
    Noyes Giannestras Biomechanics Laboratory, Department of Aerospace Engineering and Engineering Mechanics, College of Engineering, University of Cincinnati, Cincinnati, OH 45221 0048, USA
    J Biomech 33:729-35. 2000
    ..The silicone microgrooves can also be useful for delivering defined substrate strains to other adherent cells in cultures...
  17. ncbi request reprint Mechanobiology of tendon
    James H C Wang
    MechanoBiology Laboratory, Department of Orthopaedic Surgery, University of Pittsburgh, 210 Lothrop St, BST, E1647, Pittsburgh, PA 15213, USA
    J Biomech 39:1563-82. 2006
    ..Finally, future research directions in tendon mechanobiology research are discussed...
  18. ncbi request reprint Contractility affects stress fiber remodeling and reorientation of endothelial cells subjected to cyclic mechanical stretching
    J H Wang
    Department of Biomedical Engineering, Washington University, Sr Louis, MO, USA
    Ann Biomed Eng 28:1165-71. 2000
    ..We suggest that the effect of ROS on stress fiber remodeling and cell reorientation is due to the ability of ROS to regulate cellular contractility, which is crucial for these cellular responses...
  19. ncbi request reprint Substrate deformation determines actin cytoskeleton reorganization: A mathematical modeling and experimental study
    J H Wang
    Department of Aerospace Engineering and Engineering Mechanics, College of Engineering, Cincinnati, OH, 45221 0071, USA
    J Theor Biol 202:33-41. 2000
    ..The prediction was confirmed by experiments conducted on both fibroblasts and endothelial cells. The present model may be relevant for understanding better the effects of mechanical stimuli on the cells...
  20. ncbi request reprint A new method for the representation of articular surfaces using the influence surface theory of plates
    J H Wang
    Orthopaedic Biomechanics Lab, Department of Orthopaedic Surgery, Texas Tech University Health Sciences Center, El Paso, TX 79905, USA
    J Biomech 33:629-33. 2000
    ..The effectiveness of the present method was shown by reconstruction of a human femoral surface and a mathematical cone...
  21. ncbi request reprint The strain magnitude and contact guidance determine orientation response of fibroblasts to cyclic substrate strains
    J H Wang
    Department of Aerospace Engineering and Engineering Mechanics, College of Engineering, University of Cincinnati, OH 45221 0048, USA
    Connect Tissue Res 41:29-36. 2000
    ..e., strain-direction independent). It also suggested that the failure of fibroblasts to change orientations in vivo may result from the contact guidance provided by collagen fibers...
  22. doi request reprint Platelet-rich plasma releasate promotes differentiation of tendon stem cells into active tenocytes
    Jianying Zhang
    Department of Orthopaedic Surgery, University of Pittsburgh, PA 15213, USA
    Am J Sports Med 38:2477-86. 2010
    ..The aim of this study was to determine the effects of PRP, in the form of PRP-clot releasate (PRCR), on tendon stem cells (TSCs), a newly discovered cell population in tendons...
  23. pmc Intra- and inter-observer reproducibility of volume measurement of knee cartilage segmented from the OAI MR image set using a novel semi-automated segmentation method
    K T Bae
    Department of Radiology, University of Pittsburgh, Pittsburgh, PA 15213, USA
    Osteoarthritis Cartilage 17:1589-97. 2009
    ....
  24. ncbi request reprint The use of porcine small intestinal submucosa to enhance the healing of the medial collateral ligament--a functional tissue engineering study in rabbits
    Volker Musahl
    Musculoskeletal Research Center, Department of Orthopedic Surgery, University of Pittsburgh, E1641 Biomedical Science Tower, 210 Lothrop Street, P O Box 71199, Pittsburgh, PA 15213, USA
    J Orthop Res 22:214-20. 2004
    ..The objective of this study was to evaluate whether SIS can be used to enhance the healing process of a medial collateral ligament (MCL) with a gap injury in a rabbit model...
  25. ncbi request reprint Monitoring cell adhesion by using thickness shear mode acoustic wave sensors
    Fang Li
    Department of Mechanical Engineering and Material Science, University of Pittsburgh, Pittsburgh, PA 15261, United States
    Biosens Bioelectron 23:42-50. 2007
    ..The results indicate that resistance changes are related to the disruption of actin cytoskeleton and dosage-dependent...
  26. doi request reprint Cell shape regulates collagen type I expression in human tendon fibroblasts
    Fang Li
    Department of Mechanical Engineering and Materials Science, University of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA
    Cell Motil Cytoskeleton 65:332-41. 2008
    ....
  27. pmc Leukotriene B4 at low dosage negates the catabolic effect of prostaglandin E2 in human patellar tendon fibroblasts
    Bhavani P Thampatty
    MechanoBiology Laboratory, Department of Orthopaedic Surgery, University of Pittsburgh, E1641 Biomedical Science Tower 210 Lothrop Street, Pittsburgh, PA 15213, USA
    Gene 372:103-9. 2006
    ..Thus, our findings suggest that although LTB4 is generally thought to be pathogenic, low levels of LTB4 are actually beneficial in maintaining tendon tissue homeostasis...
  28. ncbi request reprint A multi-station culture force monitor system to study cellular contractility
    Brian H Campbell
    Musculoskeletal Research Center, Department of Orthopaedic Surgery, University of Pittsburgh, Pittsburgh, PA, USA
    J Biomech 36:137-40. 2003
    ..Therefore, the system can facilitate statistical design and analysis of experiments to study the effects of growth factors (e.g., TGF-betas) on cellular contraction and their potential role in scar tissue formation...
  29. ncbi request reprint A multi-station dynamic-culture force monitor system to study cell mechanobiology
    Katherin A Peperzak
    MechanoBiology Laboratory, Musculoskeletal Research Center, Department of Orthopaedic Surgery, University of Pittsburgh Medical Center, E1641 Biomedical Science Tower, 210 Lothrop Street, PO Box 71199, Pittsburgh, PA 15213, USA
    Med Eng Phys 26:355-8. 2004
    ..This new system will facilitate the study of the effects of dynamic mechanical loading on cells, remodeling of extracellular matrix, and cell-matrix interactions in vitro...
  30. ncbi request reprint Alpha-smooth muscle actin expression enhances cell traction force
    Jianxin Chen
    Department of Orthopaedic Surgery, MechanoBiology Laboratory, University of Pittsburgh, Pennsylvania 15213, USA
    Cell Motil Cytoskeleton 64:248-57. 2007
    ..Finally, CTFs were found to vary among a population of myofibroblasts, suggesting that alpha-SMA protein expression levels of individual cells also vary...
  31. pmc EP4 receptor regulates collagen type-I, MMP-1, and MMP-3 gene expression in human tendon fibroblasts in response to IL-1 beta treatment
    Bhavani P Thampatty
    MechanoBiology Laboratory, Departments of Orthopaedic Surgery and Bioengineering, University of Pittsburgh, E1640 Biomedical Science Tower, 210 Lothrop Street, Pittsburgh, PA 15213, USA
    Gene 386:154-61. 2007
    ....
  32. pmc Repetitive mechanical stretching modulates IL-1beta induced COX-2, MMP-1 expression, and PGE2 production in human patellar tendon fibroblasts
    Guoguang Yang
    MechanoBiology Laboratory, Department of Orthopaedic Surgery, University of Pittsburgh, E1641 Biomedical Science Tower, 210 Lothrop Street, Pittsburgh, PA 15213, USA
    Gene 363:166-72. 2005
    ..Thus, the results suggest that repetitive, small-magnitude stretching is anti-inflammatory, whereas large-magnitude stretching is pro-inflammatory. Therefore, moderate exercise may be beneficial to reducing tendon inflammation...
  33. ncbi request reprint Proliferation and collagen production of human patellar tendon fibroblasts in response to cyclic uniaxial stretching in serum-free conditions
    Guoguang Yang
    Musculoskeletal Research Center, Departments of Orthopaedic Surgery and Bioengineering, University of Pittsburgh Medical Center, E1641 Biomedical Science Tower, 210 Lothrop Street, PO Box 71199, Pittsburgh, PA 15213, USA
    J Biomech 37:1543-50. 2004
    ..The results suggest that mechanical stretching can modulate proliferation of human tendon fibroblasts in the absence of serum and increase the cellular production of collagen type I, which is at least in part mediated by TGF-beta1...
  34. ncbi request reprint Prostaglandin E2 affects proliferation and collagen synthesis by human patellar tendon fibroblasts
    Feridun Cilli
    MechanoBiology Laboratory, Musculoskeletal Research Center, Department of Orthopaedic Surgery, University of Pittsburgh Medical Center, Pittsburgh, PA 15213, USA
    Clin J Sport Med 14:232-6. 2004
    ..To determine the effect of prostaglandin E2 on proliferation and collagen synthesis by human patellar tendon fibroblasts...
  35. ncbi request reprint Healing and normal fibroblasts exhibit differential proliferation, collagen production, alpha-SMA expression, and contraction
    Charu Agarwal
    Department of Orthopaedic Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
    Ann Biomed Eng 34:653-9. 2006
    ..Taken together, the results of this study show that healing fibroblasts possess a differential proliferation, alpha-SMA protein expression, and contraction than normal fibroblasts...
  36. ncbi request reprint Determining substrate displacement and cell traction fields--a new approach
    Zhaochun Yang
    MechanoBiology Laboratory, Departments of Orthopaedic Surgery, Bioengineering and Mechanical Engineering, E1640 Biomedical Science Tower, 210 Lothrop Street, Pittsburgh, PA 15213, USA
    J Theor Biol 242:607-16. 2006
    ....
  37. ncbi request reprint RGD peptide-conjugated poly(dimethylsiloxane) promotes adhesion, proliferation, and collagen secretion of human fibroblasts
    Bin Li
    MechanoBiology Laboratory, Department of Orthopaedic Surgery, University of Pittsburgh, Pennsylvania 15213, USA
    J Biomed Mater Res A 79:989-98. 2006
    ..Finally, the RGD-conjugated PDMS surfaces are resistant to autoclaving and UV irradiation, which enables them to be repeatedly used in cell culture studies...
  38. ncbi request reprint A new approach to study fibroblast migration
    Bhavani P Thampatty
    MechanoBiology Laboratory, Departments of Orthopaedic Surgery, Bioengineering, and Mechanical Engineering, University of Pittsburgh, 210 Lothrop Street, Pittsburgh, PA 15213, USA
    Cell Motil Cytoskeleton 64:1-5. 2007
    ....
  39. ncbi request reprint Decreasing inflammatory response of injured patellar tendons results in increased collagen fibril diameters
    Dimosthenis A Alaseirlis
    Department of Orthopaedic Surgery, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA
    Connect Tissue Res 46:12-7. 2005
    ..Therefore, these results suggest that decreasing the inflammatory response in the early stages of tendon wound healing enhances the quality of the healing tendon through increased collagen fiber diameter and better organization...
  40. ncbi request reprint Vanadate ingestion enhances the organization and collagen fibril diameters of rat healing medical collateral ligaments
    Jianxin Chen
    MechanoBiology Laboratory, Department of Orthopaedic Surgery, University of Pittsburgh School of Medicine, 210 Lothrop Street, Biomedical Science Tower, E1641, Pittsburgh, PA 15213, USA
    Knee Surg Sports Traumatol Arthrosc 14:750-5. 2006
    ..001). These results indicate that application of vanadate may be a promising tissue engineering approach to enhance the quality of healing tissues such as injured MCLs...
  41. ncbi request reprint Development of micropost force sensor array with culture experiments for determination of cell traction forces
    Bin Li
    MechanoBiology Laboratory, Department of Orthopaedic Surgery, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA
    Cell Motil Cytoskeleton 64:509-18. 2007
    ..5 nN. Culture experiments with various types of cells showed that this MFSA technology can effectively determine CTFs of cells with different sizes and traction force magnitudes...
  42. ncbi request reprint Repeated exposure of tendon to prostaglandin-E2 leads to localized tendon degeneration
    Mustafa H Khan
    Musculoskeletal Research Center, Department of Orthopaedic Surgery, University of Pittsburgh Medical Center, 210 Lothrop Street, Pittsburgh, PA 15213, USA
    Clin J Sport Med 15:27-33. 2005
    ..To determine whether repeated exposure of rabbit patellar tendon to prostaglandin-E(2) leads to degenerative changes in the tendon...

Research Grants13

  1. An interdisciplinary and integrative study of tendinopathy
    James H C Wang; Fiscal Year: 2010
    ....
  2. An interdisciplinary and integrative study of tendinopathy
    James Wang; Fiscal Year: 2009
    ....
  3. A Request for support of 25th SPRSM Meeting
    James Wang; Fiscal Year: 2006
    ....
  4. A Multidisciplinary Study of Mechanisms for Tendinitis
    James Wang; Fiscal Year: 2005
    ..The findings from this study will also be useful in helping design experiments to study other repetitive motion disorders (e.g., carpal tunnel syndrome). ..
  5. An interdisciplinary and integrative study of tendinopathy
    James Wang; Fiscal Year: 2009
    ....