Hengbin Wang

Summary

Affiliation: University of Alabama at Birmingham
Country: USA

Publications

  1. ncbi request reprint Histone H3 and H4 ubiquitylation by the CUL4-DDB-ROC1 ubiquitin ligase facilitates cellular response to DNA damage
    Hengbin Wang
    Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Kaul Human Genetics Building Room 402A, 720 South 20th Street, 35294, USA
    Mol Cell 22:383-94. 2006
  2. ncbi request reprint Regulation of cell cycle progression and gene expression by H2A deubiquitination
    Heui Yun Joo
    Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Kaul Human Genetics Building 402A, 720 South 20th Street, Birmingham, Alabama 35294, USA
    Nature 449:1068-72. 2007
  3. pmc Regulation of histone H2A and H2B deubiquitination and Xenopus development by USP12 and USP46
    Heui Yun Joo
    Department of Biochemistry and Molecular Genetics, University of Alabama, Birmingham, Alabama 35294, USA
    J Biol Chem 286:7190-201. 2011
  4. pmc PRC2 complexes with JARID2, MTF2, and esPRC2p48 in ES cells to modulate ES cell pluripotency and somatic cell reprogramming
    Zhuo Zhang
    Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Birmingham, Alabama 35294 0024, USA
    Stem Cells 29:229-40. 2011
  5. doi request reprint In vitro and in vivo assays for studying histone ubiquitination and deubiquitination
    Ling Zhai
    Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Birmingham, AL, USA
    Methods Mol Biol 523:295-309. 2009
  6. pmc USP49 deubiquitinates histone H2B and regulates cotranscriptional pre-mRNA splicing
    Zhuo Zhang
    Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
    Genes Dev 27:1581-95. 2013
  7. pmc Purification of histone ubiquitin ligases from HeLa cells
    Amanda Jones
    Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Kaul Human Genetics Building 402A, 720 South 20th Street, Birmingham, AL 35294, United States
    Methods 54:315-25. 2011
  8. ncbi request reprint Role of Bmi1 in H2A ubiquitylation and Hox gene silencing
    Jianhua Wei
    Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
    J Biol Chem 281:22537-44. 2006
  9. doi request reprint Polycomb repressive complex 2 in embryonic stem cells: an overview
    Amanda Jones
    Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Kaul Human Genetics Building 402A, 720 South 20th Street, Birmingham, AL 35294, USA
    Protein Cell 1:1056-62. 2010
  10. ncbi request reprint Role of histone H2A ubiquitination in Polycomb silencing
    Hengbin Wang
    Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 7295, USA
    Nature 431:873-8. 2004

Collaborators

  • Jun Xu
  • Chenbei Chang
  • Yi Zhang
  • Yanming Wang
  • Li Chen Wu
  • Yue Xiong
  • Daniel Wu
  • C Patterson
  • Kathrin Plath
  • Kevin Struhl
  • Heui Yun Joo
  • Hediye Erdjument-Bromage
  • Paul Tempst
  • Amanda Jones
  • Zhuo Zhang
  • Ling Zhai
  • Ru Cao
  • Jia Fang
  • Qin Feng
  • Tim M Townes
  • Chunying Yang
  • Li Xia
  • Huck Hui Ng
  • Yaxu Wu
  • Jianhua Wei
  • Liu Yang
  • MATTHEW RENFROW
  • Ligeng Ma
  • Hang He
  • Dewang Zhou
  • Ying Cao
  • Keith E Giles
  • Shaoxia Chen
  • Wei Yang
  • Chao Li
  • Amada Jones
  • Chiao Wang Sun
  • Archer D Smith
  • Jinrong Min
  • Kevin M Pawlik
  • Woody Robbins
  • Yahong Guo
  • Zu Wen Sun
  • Mahesh B Chandrasekharan
  • Matthew R Mysliwiec
  • Matthew B Renfrow
  • Qian Dai
  • Youngsook Lee
  • Kaimao Liu
  • Rusty Kelley
  • Holly McDonough
  • Rongqin Ren
  • Jin He
  • James Meeker
  • James E Ferguson
  • Peter C Charles
  • Shuyi Nie
  • Jeffrey A Simon
  • Liangjun Wang
  • Dennis D Hickstein
  • Richard S Jones
  • Howard A Chansky
  • William H Schubach
  • Carrie S Ketel

Detail Information

Publications20

  1. ncbi request reprint Histone H3 and H4 ubiquitylation by the CUL4-DDB-ROC1 ubiquitin ligase facilitates cellular response to DNA damage
    Hengbin Wang
    Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Kaul Human Genetics Building Room 402A, 720 South 20th Street, 35294, USA
    Mol Cell 22:383-94. 2006
    ..Thus, our studies uncover CUL4-DDB-ROC1 as a histone ubiquitin ligase and demonstrate that histone H3 and H4 ubiquitylation participates in the cellular response to DNA damage...
  2. ncbi request reprint Regulation of cell cycle progression and gene expression by H2A deubiquitination
    Heui Yun Joo
    Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Kaul Human Genetics Building 402A, 720 South 20th Street, Birmingham, Alabama 35294, USA
    Nature 449:1068-72. 2007
    ..This study identifies the major deubiquitinase for histone H2A and demonstrates that H2A deubiquitination is critically involved in cell cycle progression and gene expression...
  3. pmc Regulation of histone H2A and H2B deubiquitination and Xenopus development by USP12 and USP46
    Heui Yun Joo
    Department of Biochemistry and Molecular Genetics, University of Alabama, Birmingham, Alabama 35294, USA
    J Biol Chem 286:7190-201. 2011
    ..Taken together, this study identifies USP12 and USP46 as histone deubiquitinases for H2A and H2B and reveals that USP12 regulates Xenopus development during gastrula stages...
  4. pmc PRC2 complexes with JARID2, MTF2, and esPRC2p48 in ES cells to modulate ES cell pluripotency and somatic cell reprogramming
    Zhuo Zhang
    Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Birmingham, Alabama 35294 0024, USA
    Stem Cells 29:229-40. 2011
    ....
  5. doi request reprint In vitro and in vivo assays for studying histone ubiquitination and deubiquitination
    Ling Zhai
    Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Birmingham, AL, USA
    Methods Mol Biol 523:295-309. 2009
    ..These experimental procedures are largely based on our studies in mammalian cells. These methods should provide useful tools for studying this bulky histone modification...
  6. pmc USP49 deubiquitinates histone H2B and regulates cotranscriptional pre-mRNA splicing
    Zhuo Zhang
    Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
    Genes Dev 27:1581-95. 2013
    ..Therefore, this study identifies USP49 as a histone H2B-specific deubiquitinase and uncovers a critical role for H2B deubiquitination in cotranscriptional pre-mRNA processing events. ..
  7. pmc Purification of histone ubiquitin ligases from HeLa cells
    Amanda Jones
    Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Kaul Human Genetics Building 402A, 720 South 20th Street, Birmingham, AL 35294, United States
    Methods 54:315-25. 2011
    ..So long as an in vitro enzymatic assay can be established, the approach we describe can be easily adapted to identify other histone and non-histone modifying enzymes...
  8. ncbi request reprint Role of Bmi1 in H2A ubiquitylation and Hox gene silencing
    Jianhua Wei
    Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
    J Biol Chem 281:22537-44. 2006
    ..Intriguingly, Ring2 binds to its target regions in Bmi1 knockdown cells. Therefore, our studies reveal that Bmi1 is required for H2A ubiquitylation and suggest that H2A ubiquitylation regulates Bmi1-mediated gene expression...
  9. doi request reprint Polycomb repressive complex 2 in embryonic stem cells: an overview
    Amanda Jones
    Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Kaul Human Genetics Building 402A, 720 South 20th Street, Birmingham, AL 35294, USA
    Protein Cell 1:1056-62. 2010
    ....
  10. ncbi request reprint Role of histone H2A ubiquitination in Polycomb silencing
    Hengbin Wang
    Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 7295, USA
    Nature 431:873-8. 2004
    ..Thus, our studies identify the H2A ubiquitin ligase, and link H2A ubiquitination to Polycomb silencing...
  11. ncbi request reprint Role of histone H3 lysine 27 methylation in Polycomb-group silencing
    Ru Cao
    Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7295, USA
    Science 298:1039-43. 2002
    ..Methylation on H3-K27 facilitates binding of Polycomb (PC), a component of the PRC1 complex, to histone H3 amino-terminal tail. Thus, these studies establish a link between histone methylation and PcG-mediated gene silencing...
  12. pmc Role of hPHF1 in H3K27 methylation and Hox gene silencing
    Ru Cao
    Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7295, USA
    Mol Cell Biol 28:1862-72. 2008
    ..Therefore, our results reveal hPHF1 as a component of a novel EED-EZH2 complex and demonstrate its important role in H3K27 methylation and Hox gene silencing...
  13. ncbi request reprint Purification of histone methyltransferases from HeLa cells
    Jia Fang
    Lineberger Comprehensive Cancer Center, Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 7295, USA
    Methods Enzymol 377:213-26. 2004
  14. ncbi request reprint Molecular cloning of ESET, a novel histone H3-specific methyltransferase that interacts with ERG transcription factor
    Liu Yang
    Medical Research Service, VA Puget Sound Health Care System, University of Washington, Seattle, Washington, WA 98108, USA
    Oncogene 21:148-52. 2002
    ..Together, these findings raise the possibility that transcription factor ERG may participate in transcriptional regulation through ESET-mediated histone methylation...
  15. ncbi request reprint Role of histone H3 lysine 27 methylation in X inactivation
    Kathrin Plath
    Department of Biochemistry and Biophysics, University of California San Francisco, San Francisco, CA 94143, USA
    Science 300:131-5. 2003
    ..Together, our results suggest a role for Eed-Ezh2-mediated H3-K27 methylation during initiation of both imprinted and random X inactivation and demonstrate that H3-K27 methylation is not sufficient for silencing of the Xi...
  16. pmc PRDM6 is enriched in vascular precursors during development and inhibits endothelial cell proliferation, survival, and differentiation
    Yaxu Wu
    Carolina Cardiovascular Biology Center, University of North Carolina, Chapel Hill, NC 27599 7126, USA
    J Mol Cell Cardiol 44:47-58. 2008
    ....
  17. ncbi request reprint mAM facilitates conversion by ESET of dimethyl to trimethyl lysine 9 of histone H3 to cause transcriptional repression
    Hengbin Wang
    Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Mol Cell 12:475-87. 2003
    ..Thus, our studies establish that promoter H3-K9 trimethylation is the cause of transcriptional repression and that mAM/hAM facilitates conversion of H3-K9 dimethyl to trimethyl by ESET/SETDB1...
  18. ncbi request reprint Methylation of H3-lysine 79 is mediated by a new family of HMTases without a SET domain
    Qin Feng
    Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, 27599, USA
    Curr Biol 12:1052-8. 2002
    ..Furthermore, we found that K79 methylation level is regulated throughout the cell cycle. Thus, our studies reveal a new methylation site and define a novel family of histone lysine methyltransferase...
  19. ncbi request reprint Purification and functional characterization of SET8, a nucleosomal histone H4-lysine 20-specific methyltransferase
    Jia Fang
    Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, 27599, USA
    Curr Biol 12:1086-99. 2002
    ..Thus far, only 2 residues on histone H4 are known to be methylated. While H4-arginine 3 (H4-R3) methylation is mediated by PRMT1, the enzyme(s) responsible for H4-lysine 20 (H4-K20) methylation is not known...
  20. pmc Lysine methylation within the globular domain of histone H3 by Dot1 is important for telomeric silencing and Sir protein association
    Huck Hui Ng
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Genes Dev 16:1518-27. 2002
    ..Our results indicate that histone modifications in the core globular domain have important biological functions...

Research Grants4

  1. Role of Ubp-M and H2A deubiquitination in chromatin and cellular function
    Hengbin Wang; Fiscal Year: 2010
    ..However, due to the amount of work in this application and the uncertain outcome of these experiments, we did not list these experiments as a Specific Aim. ..
  2. Role of Ubp-M and H2A deubiquitination in chromatin and cellular function
    Hengbin Wang; Fiscal Year: 2009
    ..However, due to the amount of work in this application and the uncertain outcome of these experiments, we did not list these experiments as a Specific Aim. ..