Research Topics
Genomes and Genes
Species | Hengbin WangSummaryAffiliation: University of Alabama at Birmingham Country: USA Publications
Research Grants
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Detail Information
Publications
Histone H3 and H4 ubiquitylation by the CUL4-DDB-ROC1 ubiquitin ligase facilitates cellular response to DNA damageHengbin Wang
Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Kaul Human Genetics Building Room 402A, 720 South 20th Street, 35294, USA
Mol Cell 22:383-94. 2006..Thus, our studies uncover CUL4-DDB-ROC1 as a histone ubiquitin ligase and demonstrate that histone H3 and H4 ubiquitylation participates in the cellular response to DNA damage...
Regulation of histone H2A and H2B deubiquitination and Xenopus development by USP12 and USP46Heui Yun Joo
Department of Biochemistry and Molecular Genetics, University of Alabama, Birmingham, Alabama 35294, USA
J Biol Chem 286:7190-201. 2011..Taken together, this study identifies USP12 and USP46 as histone deubiquitinases for H2A and H2B and reveals that USP12 regulates Xenopus development during gastrula stages...- Regulation of cell cycle progression and gene expression by H2A deubiquitinationHeui Yun Joo
Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Kaul Human Genetics Building 402A, 720 South 20th Street, Birmingham, Alabama 35294, USA
Nature 449:1068-72. 2007..This study identifies the major deubiquitinase for histone H2A and demonstrates that H2A deubiquitination is critically involved in cell cycle progression and gene expression... 
PRC2 complexes with JARID2, MTF2, and esPRC2p48 in ES cells to modulate ES cell pluripotency and somatic cell reprogrammingZhuo Zhang
Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Birmingham, Alabama 35294 0024, USA
Stem Cells 29:229-40. 2011....- In vitro and in vivo assays for studying histone ubiquitination and deubiquitinationLing Zhai
Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Birmingham, AL, USA
Methods Mol Biol 523:295-309. 2009..These experimental procedures are largely based on our studies in mammalian cells. These methods should provide useful tools for studying this bulky histone modification... - Purification of histone ubiquitin ligases from HeLa cellsAmanda Jones
Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Kaul Human Genetics Building 402A, 720 South 20th Street, Birmingham, AL 35294, United States
Methods 54:315-25. 2011..So long as an in vitro enzymatic assay can be established, the approach we describe can be easily adapted to identify other histone and non-histone modifying enzymes... - Role of Bmi1 in H2A ubiquitylation and Hox gene silencingJianhua Wei
Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
J Biol Chem 281:22537-44. 2006..Intriguingly, Ring2 binds to its target regions in Bmi1 knockdown cells. Therefore, our studies reveal that Bmi1 is required for H2A ubiquitylation and suggest that H2A ubiquitylation regulates Bmi1-mediated gene expression... - Polycomb repressive complex 2 in embryonic stem cells: an overviewAmanda Jones
Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Kaul Human Genetics Building 402A, 720 South 20th Street, Birmingham, AL 35294, USA
Protein Cell 1:1056-62. 2010.... - Role of histone H3 lysine 27 methylation in Polycomb-group silencingRu Cao
Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7295, USA
Science 298:1039-43. 2002..Methylation on H3-K27 facilitates binding of Polycomb (PC), a component of the PRC1 complex, to histone H3 amino-terminal tail. Thus, these studies establish a link between histone methylation and PcG-mediated gene silencing... - Role of hPHF1 in H3K27 methylation and Hox gene silencingRu Cao
Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7295, USA
Mol Cell Biol 28:1862-72. 2008..Therefore, our results reveal hPHF1 as a component of a novel EED-EZH2 complex and demonstrate its important role in H3K27 methylation and Hox gene silencing... - Molecular cloning of ESET, a novel histone H3-specific methyltransferase that interacts with ERG transcription factorLiu Yang
Medical Research Service, VA Puget Sound Health Care System, University of Washington, Seattle, Washington, WA 98108, USA
Oncogene 21:148-52. 2002..Together, these findings raise the possibility that transcription factor ERG may participate in transcriptional regulation through ESET-mediated histone methylation... - Role of histone H2A ubiquitination in Polycomb silencingHengbin Wang
Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 7295, USA
Nature 431:873-8. 2004..Thus, our studies identify the H2A ubiquitin ligase, and link H2A ubiquitination to Polycomb silencing... - Purification of histone methyltransferases from HeLa cellsJia Fang
Lineberger Comprehensive Cancer Center, Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599-7295, USA
Methods Enzymol 377:213-26. 2004 - Methylation of H3-lysine 79 is mediated by a new family of HMTases without a SET domainQin Feng
Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, 27599, USA
Curr Biol 12:1052-8. 2002..Furthermore, we found that K79 methylation level is regulated throughout the cell cycle. Thus, our studies reveal a new methylation site and define a novel family of histone lysine methyltransferase... - Purification and functional characterization of SET8, a nucleosomal histone H4-lysine 20-specific methyltransferaseJia Fang
Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, 27599, USA
Curr Biol 12:1086-99. 2002..Thus far, only 2 residues on histone H4 are known to be methylated. While H4-arginine 3 (H4-R3) methylation is mediated by PRMT1, the enzyme(s) responsible for H4-lysine 20 (H4-K20) methylation is not known... - Lysine methylation within the globular domain of histone H3 by Dot1 is important for telomeric silencing and Sir protein associationHuck Hui Ng
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
Genes Dev 16:1518-27. 2002..Our results indicate that histone modifications in the core globular domain have important biological functions... - mAM facilitates conversion by ESET of dimethyl to trimethyl lysine 9 of histone H3 to cause transcriptional repressionHengbin Wang
Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
Mol Cell 12:475-87. 2003..Thus, our studies establish that promoter H3-K9 trimethylation is the cause of transcriptional repression and that mAM/hAM facilitates conversion of H3-K9 dimethyl to trimethyl by ESET/SETDB1... - Role of histone H3 lysine 27 methylation in X inactivationKathrin Plath
Department of Biochemistry and Biophysics, University of California San Francisco, San Francisco, CA 94143, USA
Science 300:131-5. 2003..Together, our results suggest a role for Eed-Ezh2-mediated H3-K27 methylation during initiation of both imprinted and random X inactivation and demonstrate that H3-K27 methylation is not sufficient for silencing of the Xi... - PRDM6 is enriched in vascular precursors during development and inhibits endothelial cell proliferation, survival, and differentiationYaxu Wu
Carolina Cardiovascular Biology Center, University of North Carolina, Chapel Hill, NC 27599 7126, USA
J Mol Cell Cardiol 44:47-58. 2008....
Research Grants
- Role of Ubp-M and H2A deubiquitination in chromatin and cellular functionHengbin Wang; Fiscal Year: 2010..However, due to the amount of work in this application and the uncertain outcome of these experiments, we did not list these experiments as a Specific Aim. ..
- Role of Ubp-M and H2A deubiquitination in chromatin and cellular functionHengbin Wang; Fiscal Year: 2009..However, due to the amount of work in this application and the uncertain outcome of these experiments, we did not list these experiments as a Specific Aim. ..