Ching C Wang

Summary

Affiliation: University of California
Country: USA

Publications

  1. ncbi request reprint Biochemical analysis of the 20 S proteasome of Trypanosoma brucei
    Ching C Wang
    Department of Pharmaceutical Chemistry, University of California, San Francisco, CA 94143 0446, USA
    J Biol Chem 278:15800-8. 2003
  2. ncbi request reprint Functional characterization of the 11 non-ATPase subunit proteins in the trypanosome 19 S proteasomal regulatory complex
    Ziyin Li
    Department of Pharmaceutical Chemistry, University of California, San Francisco, California 94143 0446, USA
    J Biol Chem 277:42686-93. 2002
  3. pmc Polo-like kinase guides cytokinesis in Trypanosoma brucei through an indirect means
    Zhi Li
    Department of Pharmaceutical Chemistry, University of California, San Francisco, CA 94158 2280, USA
    Eukaryot Cell 9:705-16. 2010
  4. ncbi request reprint Tousled-like kinase in a microbial eukaryote regulates spindle assembly and S-phase progression by interacting with Aurora kinase and chromatin assembly factors
    Ziyin Li
    Department of Pharmaceutical Chemistry, University of California, San Francisco, CA 94158 2280, USA
    J Cell Sci 120:3883-94. 2007
  5. pmc Coupling of posterior cytoskeletal morphogenesis to the G1/S transition in the Trypanosoma brucei cell cycle
    Xiaoming Tu
    Department of Pharmaceutical Chemistry, University of California, San Francisco, CA 94143 2280, USA
    Mol Biol Cell 16:97-105. 2005
  6. ncbi request reprint The involvement of two cdc2-related kinases (CRKs) in Trypanosoma brucei cell cycle regulation and the distinctive stage-specific phenotypes caused by CRK3 depletion
    Xiaoming Tu
    Department of Pharmaceutical Chemistry, University of California San Francisco, San Francisco, CA 94143 2280, USA
    J Biol Chem 279:20519-28. 2004
  7. pmc The multiple roles of cyclin E1 in controlling cell cycle progression and cellular morphology of Trypanosoma brucei
    Stephane Gourguechon
    Department of Pharmaceutical Chemistry, University of California, San Francisco, CA 94158 2280, USA
    J Mol Biol 368:939-50. 2007
  8. pmc A La autoantigen homologue is required for the internal ribosome entry site mediated translation of giardiavirus
    Srinivas Garlapati
    Department of Pharmaceutical Chemistry, University of California San Francisco, San Francisco, California, United States of America
    PLoS ONE 6:e18263. 2011
  9. ncbi request reprint Capped mRNA with a single nucleotide leader is optimally translated in a primitive eukaryote, Giardia lamblia
    Lei Li
    Department of Pharmaceutical Chemistry, University of California, San Francisco, California 94143 2280, USA
    J Biol Chem 279:14656-64. 2004
  10. pmc Giardiavirus internal ribosome entry site has an apparently unique mechanism of initiating translation
    Srinivas Garlapati
    Department of Pharmaceutical Chemistry, University of California San Francisco, San Francisco, California, United States of America
    PLoS ONE 4:e7435. 2009

Collaborators

  • Ziyin Li
  • Wei Li
  • Xiaoming Tu
  • Lei Li
  • Stephane Gourguechon
  • Yan Li
  • Zbynek Bozdech
  • Zachary B Mackey
  • Philip Coffino
  • Bradley J Backes
  • Vern Schramm
  • H Deng
  • Jeremy C Mottram
  • Ashesh A Saraiya
  • Srinivas Garlapati
  • Praveen Kumar
  • Mohamed Bessat
  • Lu Sun
  • Takashi Umeyama
  • Anne E Sarver
  • Agnes Rinaldo-Matthis
  • Arti Gupta
  • Jane M Carlton
  • Angamuthu Selvapandiyan
  • Yang Zang
  • Steven C Almo
  • Narsimha Rao Munagala
  • Chuan H Chang
  • Wuxian Shi
  • Narsimha Munagala
  • Jesse Wu
  • Alma L Burlingame
  • Giselle Knudsen
  • Shehre Banoo Malik
  • Joana C Silva
  • Cheryl Y Okumura
  • Sylke Muller
  • Joel B Dacks
  • Peng Wu
  • Peter Upcroft
  • Robert P Hirt
  • Geoffrey J Barton
  • Rachel Schneider
  • Pier Luigi Fiori
  • Yves Van de Peer
  • Sebastien Besteiro
  • Shelby L Bidwell
  • Ian Paulsen
  • Ying Shiung Lee
  • U Cecilia M Alsmark
  • Graham H Coombs
  • Jacqueline A Upcroft
  • Patricia J Johnson
  • Daniele Dessi
  • Pavel Dolezal
  • Diego Miranda-Saavedra
  • Kazutoyo Osoegawa
  • Corin Wing
  • Cedric Simillion
  • Brian J Haas
  • Terry R Utterback
  • Felix D Bastida-Corcuera
  • T Martin Embley
  • Tamara V Feldblyum
  • Mark T Brown
  • Katrin Henze
  • Augusto Simoes-Barbosa
  • Peter C Tyler
  • Thomas Sicheritz-Ponten
  • Christophe J Noel
  • Stepanka Vanacova
  • Jeffrey L Salisbury
  • Richard H Furneaux
  • Steven L Salzberg
  • Owen White
  • Michael Schatz
  • Pieter J de Jong
  • Rebecca L Dunne
  • Hanbang Zhang
  • Mihaela Pertea
  • Zuzana Zubacova
  • Alias J Smith
  • Mandira Mukherjee
  • Gareth D Westrop
  • Richard D Hayes
  • Mahmoud Ghanem
  • Qinghu Ren
  • Hira L Nakhasi
  • Jennifer R Wortman
  • James C Morris

Detail Information

Publications45

  1. ncbi request reprint Biochemical analysis of the 20 S proteasome of Trypanosoma brucei
    Ching C Wang
    Department of Pharmaceutical Chemistry, University of California, San Francisco, CA 94143 0446, USA
    J Biol Chem 278:15800-8. 2003
    ..These results demonstrate the importance of substrate sequence specificity of the T. brucei proteasome and highlight its biochemical divergence from the human enzyme...
  2. ncbi request reprint Functional characterization of the 11 non-ATPase subunit proteins in the trypanosome 19 S proteasomal regulatory complex
    Ziyin Li
    Department of Pharmaceutical Chemistry, University of California, San Francisco, California 94143 0446, USA
    J Biol Chem 277:42686-93. 2002
    ..This structural dispensability but functional indispensability of Rpn10 may constitute another unique aspect of the proteasomes in T. brucei...
  3. pmc Polo-like kinase guides cytokinesis in Trypanosoma brucei through an indirect means
    Zhi Li
    Department of Pharmaceutical Chemistry, University of California, San Francisco, CA 94158 2280, USA
    Eukaryot Cell 9:705-16. 2010
    ..Apparently, TbPLK has accomplished its role in guiding cytokinesis before the late S phase, presumably by phosphorylating a certain substrate(s) during S phase, which may play a critical role in initiating the subsequent cytokinesis...
  4. ncbi request reprint Tousled-like kinase in a microbial eukaryote regulates spindle assembly and S-phase progression by interacting with Aurora kinase and chromatin assembly factors
    Ziyin Li
    Department of Pharmaceutical Chemistry, University of California, San Francisco, CA 94158 2280, USA
    J Cell Sci 120:3883-94. 2007
    ..In summary, TLK1 cooperates with Aurora kinase to regulate spindle assembly and chromosome segregation, and it performs a role in DNA replication probably by regulating histone modification in trypanosomes...
  5. pmc Coupling of posterior cytoskeletal morphogenesis to the G1/S transition in the Trypanosoma brucei cell cycle
    Xiaoming Tu
    Department of Pharmaceutical Chemistry, University of California, San Francisco, CA 94143 2280, USA
    Mol Biol Cell 16:97-105. 2005
    ..This connection may provide a new insight into the mechanism of morphological maintenance of an ancient protist during its cell cycle progression...
  6. ncbi request reprint The involvement of two cdc2-related kinases (CRKs) in Trypanosoma brucei cell cycle regulation and the distinctive stage-specific phenotypes caused by CRK3 depletion
    Xiaoming Tu
    Department of Pharmaceutical Chemistry, University of California San Francisco, San Francisco, CA 94143 2280, USA
    J Biol Chem 279:20519-28. 2004
    ..The different leakiness in cell cycle regulation between two stage-specific forms of an organism provides an interesting and useful model for further understanding the evolution of cell cycle control among the eukaryotes...
  7. pmc The multiple roles of cyclin E1 in controlling cell cycle progression and cellular morphology of Trypanosoma brucei
    Stephane Gourguechon
    Department of Pharmaceutical Chemistry, University of California, San Francisco, CA 94158 2280, USA
    J Mol Biol 368:939-50. 2007
    ....
  8. pmc A La autoantigen homologue is required for the internal ribosome entry site mediated translation of giardiavirus
    Srinivas Garlapati
    Department of Pharmaceutical Chemistry, University of California San Francisco, San Francisco, California, United States of America
    PLoS ONE 6:e18263. 2011
    ..HA tagged GlLa protein was detected mainly in the cytoplasm of Giardia, thus supporting a primary role of GlLa in translation initiation in Giardiavirus...
  9. ncbi request reprint Capped mRNA with a single nucleotide leader is optimally translated in a primitive eukaryote, Giardia lamblia
    Lei Li
    Department of Pharmaceutical Chemistry, University of California, San Francisco, California 94143 2280, USA
    J Biol Chem 279:14656-64. 2004
    ..Overall, our results suggest an absence of ribosome scanning for AUG in initiating translation in Giardia. A capped mRNA with a single nucleotide leader is apparently sufficient for recruiting ribosome and initiating translation...
  10. pmc Giardiavirus internal ribosome entry site has an apparently unique mechanism of initiating translation
    Srinivas Garlapati
    Department of Pharmaceutical Chemistry, University of California San Francisco, San Francisco, California, United States of America
    PLoS ONE 4:e7435. 2009
    ..These three proteins could be involved in facilitating small ribosome recruitment for initiating translation...
  11. ncbi request reprint Distinct cytoskeletal modulation and regulation of G1-S transition in the two life stages of Trypanosoma brucei
    Xiaoming Tu
    Department of Pharmaceutical Chemistry, University of California, 600 16th Street, San Francisco, CA 94143 2280, USA
    J Cell Sci 118:4353-64. 2005
    ..There is thus a major distinction in the mechanisms regulating G1-S transition and posterior morphogenesis between the two life stages of T. brucei...
  12. pmc Identification of a novel chromosomal passenger complex and its unique localization during cytokinesis in Trypanosoma brucei
    Ziyin Li
    Department of Pharmaceutical Chemistry, University of California San Francisco, San Francisco, California, United States of America
    PLoS ONE 3:e2354. 2008
    ....
  13. pmc The chromosomal passenger complex and a mitotic kinesin interact with the Tousled-like kinase in trypanosomes to regulate mitosis and cytokinesis
    Ziyin Li
    Department of Pharmaceutical Chemistry, University of California San Francisco, San Francisco, California, United States of America
    PLoS ONE 3:e3814. 2008
    ....
  14. pmc The profile of snoRNA-derived microRNAs that regulate expression of variant surface proteins in Giardia lamblia
    Wei Li
    Department of Pharmaceutical Chemistry, University of California, San Francisco, CA 94158 2280, USA
    Cell Microbiol 14:1455-73. 2012
    ..Of the total 220 vsp genes in Giardia, we have now found 178 of them carrying putative binding sites for all the miRNAs that have been currently identified, suggesting that miRNAs are likely the regulators of VSP expression in Giardia...
  15. pmc A microRNA derived from an apparent canonical biogenesis pathway regulates variant surface protein gene expression in Giardia lamblia
    Ashesh A Saraiya
    Department of Pharmaceutical Chemistry, University of California San Francisco, San Francisco, California 94158 2280, USA
    RNA 17:2152-64. 2011
    ....
  16. ncbi request reprint An aurora kinase homologue is involved in regulating both mitosis and cytokinesis in Trypanosoma brucei
    Xiaoming Tu
    Department of Pharmaceutical Chemistry, University of California, San Francisco, California 94143, USA
    J Biol Chem 281:9677-87. 2006
    ..TbAUK1 is also the first identified protein that couples both mitosis and kinetoplast replication with cytokinesis in the trypanosome...
  17. ncbi request reprint Okadaic acid overcomes the blocked cell cycle caused by depleting Cdc2-related kinases in Trypanosoma brucei
    Ziyin Li
    Department of Pharmaceutical Chemistry, University of California, San Francisco, CA 94143 2280, USA
    Exp Cell Res 312:3504-16. 2006
    ..These observations support the abovementioned assumption and suggest the presence of a novel OKA-sensitive protein(s) regulating kinetoplast replication that still remains to be identified...
  18. pmc Transition of a microRNA from repressing to activating translation depending on the extent of base pairing with the target
    Ashesh A Saraiya
    Department of Pharmaceutical Chemistry, University of California San Francisco, San Francisco, California, United States of America
    PLoS ONE 8:e55672. 2013
    ..This is the first report showing a correlation between the extent of base-pairing with the target and a change in miRNA function...
  19. ncbi request reprint Depletion of anaphase-promoting complex or cyclosome (APC/C) subunit homolog APC1 or CDC27 of Trypanosoma brucei arrests the procyclic form in metaphase but the bloodstream form in anaphase
    Praveen Kumar
    Department of Pharmaceutical Chemistry, University of California, San Francisco, California 94143 2280, USA
    J Biol Chem 280:31783-91. 2005
    ..The apparent lack of functional involvement of some of the other individual structural subunit homologs of APC/C may indicate the structural uniqueness of T. brucei APC/C...
  20. ncbi request reprint The purine nucleoside phosphorylase from Trichomonas vaginalis is a homologue of the bacterial enzyme
    Narsimha Munagala
    Department of Pharmaceutical Chemistry, University of California San Francisco, San Francisco, California 94143 0446, USA
    Biochemistry 41:10382-9. 2002
    ..vaginalis PNP with a K(is) of 2.3 microM by competing with adenosine. T. vaginalis PNP thus belongs to the family of bacterial PNPs and constitutes a target for antitrichomonial chemotherapy...
  21. pmc CRK9 contributes to regulation of mitosis and cytokinesis in the procyclic form of Trypanosoma brucei
    Stephane Gourguechon
    Department of Pharmaceutical Chemistry, University of California, San Francisco, CA 94158 2280, USA
    BMC Cell Biol 10:68. 2009
    ..The Trypanosoma brucei cell cycle is regulated by combinations of cyclin/CRKs (cdc2 related kinases). Recently, two additional cyclins (CYC10, CYC11) and six new CRK (CRK7-12) homologues were identified in the T. brucei genome database 12...
  22. pmc Structural elements in the 5'-untranslated region of giardiavirus transcript essential for internal ribosome entry site-mediated translation initiation
    Srinivas Garlapati
    Department of Pharmaceutical Chemistry, University of California, San Francisco, California 94107 2280, USA
    Eukaryot Cell 4:742-54. 2005
    ..Together with the previous results, we have now completed analysis of the entire structure of GLV IRES and fully defined the functionally essential structural elements in it...
  23. pmc The structural basis of localizing polo-like kinase to the flagellum attachment zone in Trypanosoma brucei
    Lu Sun
    Department of Pharmaceutical Chemistry, University of California San Francisco, San Francisco, California, United States of America
    PLoS ONE 6:e27303. 2011
    ..A putative cryptic bipartite nuclear targeting signal was identified in TbPlk, which was capable of directing TbPlk into the nucleus when either the kinase activity was lost or the PB1 was deleted from the protein...
  24. pmc Identification of a bacterial-like HslVU protease in the mitochondria of Trypanosoma brucei and its role in mitochondrial DNA replication
    Ziyin Li
    Department of Pharmaceutical Chemistry, University of California, San Francisco, California, United States of America
    PLoS Pathog 4:e1000048. 2008
    ..TbHslVU is a eubacterial protease identified in the mitochondria of a eukaryote. It has a novel function in regulating mitochondrial DNA replication that has never been observed in other organisms...
  25. pmc Identification in the ancient protist Giardia lamblia of two eukaryotic translation initiation factor 4E homologues with distinctive functions
    Lei Li
    Department of Pharmaceutical Chemistry, University of California San Francisco, N572C, Mission Bay Genentech Hall, 600 16th St, San Francisco, CA 94122 2280, USA
    Eukaryot Cell 4:948-59. 2005
    ....
  26. pmc Pairwise knockdowns of cdc2-related kinases (CRKs) in Trypanosoma brucei identified the CRKs for G1/S and G2/M transitions and demonstrated distinctive cytokinetic regulations between two developmental stages of the organism
    Xiaoming Tu
    Department of Pharmaceutical Chemistry, University of California, San Francisco, California 94143 2280, USA
    Eukaryot Cell 4:755-64. 2005
    ..The two different cytokinetic regulations between two distinct stage-specific forms of the same organism may provide an interesting and useful model for further understanding the evolution of cytokinetic control among eukaryotes...
  27. pmc Polo-like kinase is expressed in S/G2/M phase and associated with the flagellum attachment zone in both procyclic and bloodstream forms of Trypanosoma brucei
    Takashi Umeyama
    Department of Pharmaceutical Chemistry, University of California, San Francisco, CA 94158 2280, USA
    Eukaryot Cell 7:1582-90. 2008
    ..An indicated TbPLK-EYFP association with the FAZs of G(2)/M cells may thus represent a timely localization to a potential initiation site of cytokinesis, which agrees with the recognized role of TbPLK in cytokinetic initiation...
  28. pmc KMP-11, a basal body and flagellar protein, is required for cell division in Trypanosoma brucei
    Ziyin Li
    Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, CA 94158 2280, USA
    Eukaryot Cell 7:1941-50. 2008
    ..Its distinct roles in regulating FAZ formation in the two forms may provide a clue to the different mechanisms of cytokinetic initiation in procyclic and bloodstream trypanosomes...
  29. pmc Experimental verification of the identity of variant-specific surface proteins in Giardia lamblia trophozoites
    Wei Li
    Department of Pharmaceutical Chemistry, University of California, San Francisco, CA, USA
    MBio 4:e00321-13. 2013
    ..By these criteria, 73 genes are identified in the Giardia WB strain genome database as the putative repertoire of VSPs...
  30. pmc Gene regulation in Giardia lambia involves a putative microRNA derived from a small nucleolar RNA
    Wei Li
    Department of Pharmaceutical Chemistry, University of California San Francisco, San Francisco, California, United States of America
    PLoS Negl Trop Dis 5:e1338. 2011
    ..miR5 was found to inhibit Luciferase expression (∼20%) of transcripts carrying these potential target sites, indicating that snoRNA-derived miRNA can regulate the expression of multiple genes in Giardia...
  31. ncbi request reprint The adenine phosphoribosyltransferase from Giardia lamblia has a unique reaction mechanism and unusual substrate binding properties
    Anne E Sarver
    Department of Pharmaceutical Chemistry, University of California, San Francisco, California 94143 0446, USA
    J Biol Chem 277:39973-80. 2002
    ..An ordered substrate binding in the reverse reaction with AMP bound first followed by PP(i) is thus postulated...
  32. ncbi request reprint A PHO80-like cyclin and a B-type cyclin control the cell cycle of the procyclic form of Trypanosoma brucei
    Ziyin Li
    Department of Pharmaceutical Chemistry, University of California, San Francisco, California 94143 0446, USA
    J Biol Chem 278:20652-8. 2003
    ..This observation indicates an uncoupling between the kinetoplast and the nuclear cycle, resulting in cell division driven by kinetoplast segregation with neither a priori S phase nor mitosis in the trypanosome...
  33. pmc A minimal anaphase promoting complex/cyclosome (APC/C) in Trypanosoma brucei
    Mohamed Bessat
    Department of Pharmaceutical Chemistry, University of California San Francisco, San Francisco, California, United States of America
    PLoS ONE 8:e59258. 2013
    ..In all, a 10-subunit APC/C machinery with a conserved function is identified in T. brucei without linking to a MCC-like complex, thus indicating a unique T. brucei APC/C...
  34. pmc snoRNA, a novel precursor of microRNA in Giardia lamblia
    Ashesh A Saraiya
    Department of Pharmaceutical Chemistry, University of California San Francisco, San Francisco, California, USA
    PLoS Pathog 4:e1000224. 2008
    ..Additionally, the level of miR2 was significantly reduced when Dicer was knocked down. In all, the evidence indicates the presence of a snoRNA-derived miRNA-mediated translational repression in Giardia...
  35. ncbi request reprint Differentiation of Trypanosoma brucei may be stage non-specific and does not require progression of cell cycle
    Yan Li
    Department of Pharmaceutical Chemistry, University of California, Genentech Hall, 600 16th Street, San Francisco, CA 94143 2280, USA
    Mol Microbiol 49:251-65. 2003
    ..brucei cell cycle. The process is probably a simple pattern change of gene expression in the trypanosome induced by a temperature decrease from 37 degrees C to 26 degrees C in the presence of citrate and cis-aconitate...
  36. ncbi request reprint Identification of a novel internal ribosome entry site in giardiavirus that extends to both sides of the initiation codon
    Srinivas Garlapati
    Department of Pharmaceutical Chemistry, University of California, San Francisco, California 94143 2280, USA
    J Biol Chem 279:3389-97. 2004
    ..This is, to our knowledge, the first reported identification of a viral IRES with an estimated size of 517 nts that extends to both sides of the initiation site...
  37. ncbi request reprint A likely molecular basis of the susceptibility of Giardia lamblia towards oxygen
    Lei Li
    Department of Pharmaceutical Chemistry, University of California, San Francisco, CA 94143 2280, USA
    Mol Microbiol 59:202-11. 2006
    ..Giardia NADH oxidase could be thus an instrumental enzyme for the organism to adapt to and to tolerate an aerobic living environment...
  38. ncbi request reprint Adenosine is the primary precursor of all purine nucleotides in Trichomonas vaginalis
    Narsimha Rao Munagala
    Department of Pharmaceutical Chemistry, School of Pharmacy, University of California, 513 Parnassus Avenue, San Francisco, CA 94143 0446, USA
    Mol Biochem Parasitol 127:143-9. 2003
    ..vaginalis and the purine nucleoside kinase one of the most pivotal enzymes in purine salvage and a potential target for anti-trichomoniasis chemotherapy...
  39. pmc The microRNAs in an ancient protist repress the variant-specific surface protein expression by targeting the entire coding sequence
    Ashesh A Saraiya
    Department of Pharmaceutical Chemistry, University of California San Francisco, San Francisco, California, United States of America
    PLoS Pathog 10:e1003791. 2014
    ....
  40. ncbi request reprint An easily dissociated 26 S proteasome catalyzes an essential ubiquitin-mediated protein degradation pathway in Trypanosoma brucei
    Ziyin Li
    Department of Pharmaceutical Chemistry, University of California, San Francisco, California 94143 0446, USA
    J Biol Chem 277:15486-98. 2002
    ..The T. brucei 11 S regulator (PA26)-deficient RNA interference cells grew normally, suggesting the dispensability of activated 20 S proteasome in T. brucei...
  41. ncbi request reprint Closed site complexes of adenine phosphoribosyltransferase from Giardia lamblia reveal a mechanism of ribosyl migration
    Wuxian Shi
    Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Biol Chem 277:39981-8. 2002
    ..1-A excursion of the ribosyl anomeric carbon, whereas the adenine ring and the 5-phosphoryl group remained fixed. G. lamblia APRTase therefore provides another example of nucleophilic displacement by electrophile migration...
  42. pmc Centrin1 is required for organelle segregation and cytokinesis in Trypanosoma brucei
    Angamuthu Selvapandiyan
    Division of Emerging and Transfusion Transmitted Diseases, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892, USA
    Mol Biol Cell 18:3290-301. 2007
    ..brucei...
  43. ncbi request reprint Identification of a subversive substrate of Trichomonas vaginalis purine nucleoside phosphorylase and the crystal structure of the enzyme-substrate complex
    Yang Zang
    Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 14853 1301, USA
    J Biol Chem 280:22318-25. 2005
    ..5 A as compared with the binding scheme of F-dAdo in E. coli PNP. The structures of the TvPNP complexes suggest opportunities for further improved subversive substrates beyond F-dAdo...
  44. pmc Draft genome sequence of the sexually transmitted pathogen Trichomonas vaginalis
    Jane M Carlton
    Institute for Genomic Research, 9712 Medical Research Drive, Rockville, MD 20850, USA
    Science 315:207-12. 2007
    ..The genome sequence predicts previously unknown functions for the hydrogenosome, which support a common evolutionary origin of this unusual organelle with mitochondria...
  45. pmc Inhibition and structure of Trichomonas vaginalis purine nucleoside phosphorylase with picomolar transition state analogues
    Agnes Rinaldo-Matthis
    Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Biochemistry 46:659-68. 2007
    ..And this difference is explained by isotope-edited difference infrared spectroscopy with [6-18O]ImmH to establish that O6 is the keto tautomer in TvPNP x ImmH x PO4, causing an unfavorable leaving-group interaction...