Kylie Walters

Summary

Affiliation: University of Minnesota
Country: USA

Publications

  1. pmc MTMDAT-HADDOCK: high-throughput, protein complex structure modeling based on limited proteolysis and mass spectrometry
    Janosch Hennig
    Department of Physics, Chemistry, and Biology, Linkoping University, SE 581 83 Linkoping, Sweden
    BMC Struct Biol 12:29. 2012
  2. pmc DNA-repair protein hHR23a alters its protein structure upon binding proteasomal subunit S5a
    Kylie J Walters
    Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN 55455, USA
    Proc Natl Acad Sci U S A 100:12694-9. 2003
  3. doi request reprint Rpn10 protects the proteasome from Dsk2
    Kylie J Walters
    Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Minneapolis, MN 55455, USA
    Mol Cell 32:459-60. 2008
  4. pmc Ubiquitin receptor proteins hHR23a and hPLIC2 interact
    Yang Kang
    Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN 55455, USA
    J Mol Biol 365:1093-101. 2007
  5. ncbi request reprint Ubiquitin recognition by the DNA repair protein hHR23a
    Qinghua Wang
    Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, Minnesota 55455, USA
    Biochemistry 42:13529-35. 2003
  6. pmc Defining how ubiquitin receptors hHR23a and S5a bind polyubiquitin
    Yang Kang
    Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN 55455, USA
    J Mol Biol 369:168-76. 2007
  7. pmc Structure of the s5a:k48-linked diubiquitin complex and its interactions with rpn13
    Naixia Zhang
    Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Minneapolis, 55455, USA
    Mol Cell 35:280-90. 2009
  8. pmc Structure of proteasome ubiquitin receptor hRpn13 and its activation by the scaffolding protein hRpn2
    Xiang Chen
    Department of Biochemistry, University of Minnesota, Minneapolis, MN 55455, USA
    Mol Cell 38:404-15. 2010
  9. pmc The SH3 domain of a M7 interacts with its C-terminal proline-rich region
    Qinghua Wang
    Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN 55455, USA
    Protein Sci 16:189-96. 2007
  10. ncbi request reprint Structure of S5a bound to monoubiquitin provides a model for polyubiquitin recognition
    Qinghua Wang
    Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, 6 155 Jackson Hall, 321 Church St SE, Minneapolis, MN 55455, USA
    J Mol Biol 348:727-39. 2005

Collaborators

Detail Information

Publications27

  1. pmc MTMDAT-HADDOCK: high-throughput, protein complex structure modeling based on limited proteolysis and mass spectrometry
    Janosch Hennig
    Department of Physics, Chemistry, and Biology, Linkoping University, SE 581 83 Linkoping, Sweden
    BMC Struct Biol 12:29. 2012
    ..Here, we introduce a pipeline between MTMDAT and HADDOCK, that facilitates protein-protein complex structure probing in a high-throughput and highly automated fashion...
  2. pmc DNA-repair protein hHR23a alters its protein structure upon binding proteasomal subunit S5a
    Kylie J Walters
    Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN 55455, USA
    Proc Natl Acad Sci U S A 100:12694-9. 2003
    ..Interestingly, binding of the proteasomal subunit S5a disrupts the hHR23a interdomain interactions and thereby causes it to adopt an opened conformation...
  3. doi request reprint Rpn10 protects the proteasome from Dsk2
    Kylie J Walters
    Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Minneapolis, MN 55455, USA
    Mol Cell 32:459-60. 2008
    ..In a recent issue of Molecular Cell, Matiuhin et al. (2008) reveal how the ubiquitin receptor Dsk2 becomes deleterious to cells and that it is kept in check by Rpn10, which restricts its access to the proteasome...
  4. pmc Ubiquitin receptor proteins hHR23a and hPLIC2 interact
    Yang Kang
    Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN 55455, USA
    J Mol Biol 365:1093-101. 2007
    ..In addition, we demonstrate that these two proteins associate in mammalian cells. Intriguingly, inhibition of the proteasome mitigates hHR23a/hPLIC2 interaction...
  5. ncbi request reprint Ubiquitin recognition by the DNA repair protein hHR23a
    Qinghua Wang
    Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, Minnesota 55455, USA
    Biochemistry 42:13529-35. 2003
    ..HHR23 proteins are hypothesized to link ubiquitin to S5a, and we provide direct evidence that hHR23 could form a ternary complex with ubiquitin and S5a...
  6. pmc Defining how ubiquitin receptors hHR23a and S5a bind polyubiquitin
    Yang Kang
    Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN 55455, USA
    J Mol Biol 369:168-76. 2007
    ..Furthermore, we demonstrate that hHR23a is surprisingly adept at sequestering the ubiquitin moieties of a polyubiquitin chain, and provide evidence that it and the ubiquitylated substrate are committed to each other after binding...
  7. pmc Structure of the s5a:k48-linked diubiquitin complex and its interactions with rpn13
    Naixia Zhang
    Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Minneapolis, 55455, USA
    Mol Cell 35:280-90. 2009
    ..Altogether, our data demonstrate that S5a is highly adaptive and cooperative toward binding ubiquitin chains...
  8. pmc Structure of proteasome ubiquitin receptor hRpn13 and its activation by the scaffolding protein hRpn2
    Xiang Chen
    Department of Biochemistry, University of Minnesota, Minneapolis, MN 55455, USA
    Mol Cell 38:404-15. 2010
    ..Altogether, our results provide mechanistic insights into hRpn13's functional activities with Uch37 and ubiquitin and suggest that its role as a ubiquitin receptor is finely tuned for proteasome targeting...
  9. pmc The SH3 domain of a M7 interacts with its C-terminal proline-rich region
    Qinghua Wang
    Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN 55455, USA
    Protein Sci 16:189-96. 2007
    ..The interaction may affect the orientation of the tail without sacrificing the availability of the canonical "PxxP"-binding surface...
  10. ncbi request reprint Structure of S5a bound to monoubiquitin provides a model for polyubiquitin recognition
    Qinghua Wang
    Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, 6 155 Jackson Hall, 321 Church St SE, Minneapolis, MN 55455, USA
    J Mol Biol 348:727-39. 2005
    ..On the basis of these results we present a model for how S5a and other ubiquitin-binding proteins recognize polyubiquitin...
  11. ncbi request reprint Ubiquitin family proteins and their relationship to the proteasome: a structural perspective
    Kylie J Walters
    Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN 55455, USA
    Biochim Biophys Acta 1695:73-87. 2004
    ..In this chapter, we provide a structural perspective of how the ubiquitin family of proteins interacts with the proteasome...
  12. ncbi request reprint UBL/UBA ubiquitin receptor proteins bind a common tetraubiquitin chain
    Yang Kang
    Department of Biochemistry, Molecular Biology, University of Minnesota, Minneapolis, MN 55455, USA
    J Mol Biol 356:1027-35. 2006
    ..Altogether our results suggest a mechanism through which UBL/UBA proteins could protect chains from premature de-ubiquitylation and unnecessary elongation during their transit to the proteasome...
  13. pmc Prokaryotic ubiquitin-like protein pup is intrinsically disordered
    Xiang Chen
    Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, 55455, USA
    J Mol Biol 392:208-17. 2009
    ..Moreover, Mpa selected one configuration for a region undergoing chemical exchange in the free protein. These findings provide mechanistic insights into Pup's functional role as a degradation signal...
  14. ncbi request reprint Arylamine N-acetyltransferase aggregation and constitutive ubiquitylation
    Fen Liu
    Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN 55455, USA
    J Mol Biol 361:482-92. 2006
    ..Altogether, we provide fundamental information on why humans harboring certain NAT variants exhibit reduced acetylation capabilities...
  15. pmc A critical role for the loop region of the basic helix-loop-helix/leucine zipper protein Mlx in DNA binding and glucose-regulated transcription
    Lin Ma
    Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN 55455, USA
    Nucleic Acids Res 35:35-44. 2007
    ..In summary, our results support a model in which the loop regions of Mlx play an important functional role in mediating the coordinate binding of ChREBP/Mlx heterodimers to the ChoRE...
  16. ncbi request reprint Arylamine N-acetyltransferases: characterization of the substrate specificities and molecular interactions of environmental arylamines with human NAT1 and NAT2
    Li Liu
    Department of Medicinal Chemistry, University of Minnesota, 308 Harvard Street SE, Minneapolis, MN 55455, USA
    Chem Res Toxicol 20:1300-8. 2007
    ..These results provide insight into the structural basis for the substrate specificity of two NATs that play major roles in the biotransformation of genotoxic environmental arylamines...
  17. doi request reprint Multitasking with ubiquitin through multivalent interactions
    Fen Liu
    Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, Minnesota 55455, USA
    Trends Biochem Sci 35:352-60. 2010
    ..Multivalent interactions regulate each stage of ubiquitin signaling pathways, and appear within the ubiquitin signal, the ubiquitylated substrate, ubiquitin processing enzymes and ubiquitin recognition proteins...
  18. pmc Probing the catalytic potential of the hamster arylamine N-acetyltransferase 2 catalytic triad by site-directed mutagenesis of the proximal conserved residue, Tyr190
    Xin Zhou
    Department of Medicinal Chemistry, University of Minnesota, Minneapolis, MN 55455, USA
    FEBS J 276:6928-41. 2009
    ..These results suggest that NAT2 catalytic efficiency is partially governed by the ability of Tyr190 to mediate the collective impact of multiple side chains on the electrostatic potential and local conformation of the active site...
  19. ncbi request reprint NMR-based model reveals the structural determinants of mammalian arylamine N-acetyltransferase substrate specificity
    Naixia Zhang
    Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN 55455, USA
    J Mol Biol 363:188-200. 2006
    ..Our results represent an important step toward predicting whether arylamines present in new products can be detoxified by mammalian NATs...
  20. pmc Insights into how protein dynamics affects arylamine N-acetyltransferase catalysis
    Naixia Zhang
    Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN 55455, USA
    Biochem Biophys Res Commun 385:395-401. 2009
    ..Our NMR data on a catalytically active state of hamster NAT2 suggest that structural rearrangements caused by its acetylation might contribute to this protection...
  21. ncbi request reprint Chemical shift assignments of the (poly)ubiquitin-binding region of the proteasome subunit S5a
    Qinghua Wang
    J Biomol NMR 30:231-2. 2004
  22. pmc Ubiquitin docking at the proteasome through a novel pleckstrin-homology domain interaction
    Patrick Schreiner
    Center for Integrated Protein Science at the Department Chemie, Lehrstuhl für Biochemie, Technische Universitat Munchen, Lichtenbergstrasse 4, D 85747 Garching, Germany
    Nature 453:548-52. 2008
    ..Finally, we provide a model structure of Rpn13 complexed to diubiquitin, which provides insights into how Rpn13 as a ubiquitin receptor is coupled to substrate deubiquitination by Uch37...
  23. pmc Proteasome subunit Rpn13 is a novel ubiquitin receptor
    Koraljka Husnjak
    Institute of Biochemistry II and Cluster of Excellence Macromolecular Complexes, Goethe University, Theodor Stern Kai 7, D 60590 Frankfurt Main, Germany
    Nature 453:481-8. 2008
    ..Because Rpn13 is also the proteasomal receptor for Uch37, a deubiquitinating enzyme, our findings suggest a coupling of chain recognition and disassembly at the proteasome...
  24. pmc Components of the ubiquitin-proteasome pathway compete for surfaces on Rad23 family proteins
    Amanda M Goh
    Department of Pathology, Harvard Medical School, Boston, Massachusetts, USA
    BMC Biochem 9:4. 2008
    ....
  25. ncbi request reprint Structural studies of the interaction between ubiquitin family proteins and proteasome subunit S5a
    Kylie J Walters
    Department of Pathology and Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Biochemistry 41:1767-77. 2002
    ..This study provides structural insights into protein recognition by the proteasome, and illustrates how the protein surface of a commonly utilized fold has highly evolved for various biological roles...
  26. ncbi request reprint Ufd1 exhibits dual ubiquitin binding modes
    Kylie J Walters
    Structure 13:943-4. 2005

Research Grants14

  1. Structural Analysis of NAT Acetylation, Substrate Specificity and Polymorphisms
    Kylie J Walters; Fiscal Year: 2010
    ..We determine how certain arylamines evade NAT detoxification and why the NAT proteins of some people are destroyed before they can perform their protein function. ..
  2. Defining how the Proteasome Recognizes its Ubiquitylated Substrates
    Kylie Walters; Fiscal Year: 2009
    ..Such knowledge is the first step towards rationally designing inhibitors for specific protein substrates, which in the long-term could be used clinically with few side effects. ..
  3. Defining how the Proteasome Recognizes its Ubiquitylated Substrates
    Kylie J Walters; Fiscal Year: 2010
    ..Such knowledge is the first step towards rationally designing inhibitors for specific protein substrates, which in the long-term could be used clinically with few side effects. ..
  4. Defining how the Proteasome Recognizes its Ubiquitylated Substrates
    Kylie Walters; Fiscal Year: 2009
    ..Such knowledge is the first step towards rationally designing inhibitors for specific protein substrates, which in the long-term could be used clinically with few side effects. ..
  5. Structural Analysis of NAT Acetylation, Substrate Specificity and Polymorphisms
    Kylie Walters; Fiscal Year: 2007
    ..We determine how certain arylamines evade NAT detoxification and why the NAT proteins of some people are destroyed before they can perform their protein function. ..
  6. Linking proteasome activity to DNA repair through hHR23
    Kylie Walters; Fiscal Year: 2007
    ..This work is expected to yield fundamental knowledge of the mechanism behind the hHR23 interaction with the ubiquitin-proteasome pathway and how this interaction functions in nucleotide excision repair. ..
  7. NMR Structural Studies of Ubiquitin Receptor Protein Complexes
    Kylie J Walters; Fiscal Year: 2010
    ..The outcome of this proposal will afford fundamental information on how the proteasome degrades its substrates and factors involved in determining whether ubiquitinated proteins are fated for degradation. ..