P Walter

Summary

Affiliation: University of California
Country: USA

Publications

  1. pmc IRE1-independent gain control of the unfolded protein response
    Jess H Leber
    Department of Biochemistry and Biophysics, University of California, San Francisco, USA
    PLoS Biol 2:E235. 2004
  2. pmc Walking along the serendipitous path of discovery
    Peter Walter
    Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94143 2200, USA
    Mol Biol Cell 21:15-7. 2010
  3. pmc On the mechanism of sensing unfolded protein in the endoplasmic reticulum
    Joel J Credle
    Howard Hughes Medical Institute, Departments of Biochemistry and Biophysics and Medicine, University of California, San Francisco, CA 94143 2200, USA
    Proc Natl Acad Sci U S A 102:18773-84. 2005
  4. pmc Co-translational protein targeting catalyzed by the Escherichia coli signal recognition particle and its receptor
    T Powers
    Department of Biochemistry and Biophysics, University of California, School of Medicine, San Francisco 94143, USA
    EMBO J 16:4880-6. 1997
  5. ncbi request reprint Role of 4.5S RNA in assembly of the bacterial signal recognition particle with its receptor
    P Peluso
    Howard Hughes Medical Institute and Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94143, USA
    Science 288:1640-3. 2000
  6. ncbi request reprint The transmembrane kinase Ire1p is a site-specific endonuclease that initiates mRNA splicing in the unfolded protein response
    C Sidrauski
    Department of Biochemistry and Biophysics, University of California, School of Medicine, San Francisco 94143 0448, USA
    Cell 90:1031-9. 1997
  7. ncbi request reprint SRP samples nascent chains for the presence of signal sequences by interacting with ribosomes at a discrete step during translation elongation
    S C Ogg
    Department of Biochemistry and Biophysics Medical School, University of California, San Francisco 94143 0448, USA
    Cell 81:1075-84. 1995
  8. pmc The signal recognition particle (SRP) RNA links conformational changes in the SRP to protein targeting
    Niels Bradshaw
    Howard Hughes Medical Institute, Department of Biochemistry and Biophysics, University of California at San Francisco, CA 94158, USA
    Mol Biol Cell 18:2728-34. 2007
  9. pmc X-ray structures of the signal recognition particle receptor reveal targeting cycle intermediates
    Christopher L Reyes
    Graduate Group in Biophysics, Department of Biochemistry and Biophysics, University of California at San Francisco, San Francisco, California, United States of America
    PLoS ONE 2:e607. 2007
  10. pmc NMR studies of the most conserved RNA domain of the mammalian signal recognition particle (SRP)
    U Schmitz
    Department of Pharmaceutical Chemistry, University of California, San Francisco 94143 0446, USA
    RNA 2:1213-27. 1996

Collaborators

Detail Information

Publications83

  1. pmc IRE1-independent gain control of the unfolded protein response
    Jess H Leber
    Department of Biochemistry and Biophysics, University of California, San Francisco, USA
    PLoS Biol 2:E235. 2004
    ..We show that by adding an additional signaling element to the basic UPR circuit, a simple switch is transformed into a complex response...
  2. pmc Walking along the serendipitous path of discovery
    Peter Walter
    Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94143 2200, USA
    Mol Biol Cell 21:15-7. 2010
    ..A key regulatory switch in the UPR, for example, is a cytoplasmic, nonconventional mRNA spicing reaction, initiated by a bifunctional transmembrane kinase/endoribonuclease...
  3. pmc On the mechanism of sensing unfolded protein in the endoplasmic reticulum
    Joel J Credle
    Howard Hughes Medical Institute, Departments of Biochemistry and Biophysics and Medicine, University of California, San Francisco, CA 94143 2200, USA
    Proc Natl Acad Sci U S A 102:18773-84. 2005
    ..The changes in the ER lumen in turn position Ire1 kinase domains in the cytoplasm optimally for autophosphorylation to initiate the UPR...
  4. pmc Co-translational protein targeting catalyzed by the Escherichia coli signal recognition particle and its receptor
    T Powers
    Department of Biochemistry and Biophysics, University of California, School of Medicine, San Francisco 94143, USA
    EMBO J 16:4880-6. 1997
    ..Our data illustrate the extreme functional conservation between prokaryotic and eukaryotic SRP and SRP receptors and suggest that the basic mechanism of co-translational protein targeting is conserved between bacteria and mammals...
  5. ncbi request reprint Role of 4.5S RNA in assembly of the bacterial signal recognition particle with its receptor
    P Peluso
    Howard Hughes Medical Institute and Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94143, USA
    Science 288:1640-3. 2000
    ..Surprisingly, 4.5S RNA facilitates both assembly and disassembly of the Ffh-FtsY complex to a similar extent. These results provide an example of an RNA molecule facilitating protein-protein interactions in a catalytic fashion...
  6. ncbi request reprint The transmembrane kinase Ire1p is a site-specific endonuclease that initiates mRNA splicing in the unfolded protein response
    C Sidrauski
    Department of Biochemistry and Biophysics, University of California, School of Medicine, San Francisco 94143 0448, USA
    Cell 90:1031-9. 1997
    ..The addition of purified tRNA ligase completes splicing; we therefore have reconstituted HAC1 mRNA splicing in vitro from purified components...
  7. ncbi request reprint SRP samples nascent chains for the presence of signal sequences by interacting with ribosomes at a discrete step during translation elongation
    S C Ogg
    Department of Biochemistry and Biophysics Medical School, University of California, San Francisco 94143 0448, USA
    Cell 81:1075-84. 1995
    ..These results are consistent with a sampling model in which SRP cycles on and off of translating ribosomes at specific steps during the elongation cycle to inspect all nascent chains for the presence of signal sequences...
  8. pmc The signal recognition particle (SRP) RNA links conformational changes in the SRP to protein targeting
    Niels Bradshaw
    Howard Hughes Medical Institute, Department of Biochemistry and Biophysics, University of California at San Francisco, CA 94158, USA
    Mol Biol Cell 18:2728-34. 2007
    ..In this way, the SRP RNA may coordinate the interaction of the SRP and the SR with ribosome recruitment and transfer to the translocon, explaining why the SRP RNA is an indispensable component of the protein targeting machinery...
  9. pmc X-ray structures of the signal recognition particle receptor reveal targeting cycle intermediates
    Christopher L Reyes
    Graduate Group in Biophysics, Department of Biochemistry and Biophysics, University of California at San Francisco, San Francisco, California, United States of America
    PLoS ONE 2:e607. 2007
    ..We propose that these structural changes represent discrete conformational states assumed by FtsY during targeting complex formation and dissociation...
  10. pmc NMR studies of the most conserved RNA domain of the mammalian signal recognition particle (SRP)
    U Schmitz
    Department of Pharmaceutical Chemistry, University of California, San Francisco 94143 0446, USA
    RNA 2:1213-27. 1996
    ..The bulge present in both RNA fragments exhibits a similar, pronounced flexibility in the absence of Mg2+, indicating that the additional bulge in the 43mer does not stabilize the other bulge...
  11. pmc Induced nucleotide specificity in a GTPase
    Shu ou Shan
    Howard Hughes Medical Institute and Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94143 0448, USA
    Proc Natl Acad Sci U S A 100:4480-5. 2003
    ..We propose that this conformational change leads to more accurate positioning of the nucleotide and thus could contribute to activation of FtsY's GTPase activity by a novel mechanism...
  12. ncbi request reprint A novel mechanism for regulating activity of a transcription factor that controls the unfolded protein response
    J S Cox
    Department of Biochemistry and Biophysics, University of California, School of Medicine, San Francisco 94143 0448, USA
    Cell 87:391-404. 1996
    ..We propose that the complex regulation of Hac1p expression serves to provide multiple levels at which the UPR can be controlled...
  13. ncbi request reprint Structure of the conserved GTPase domain of the signal recognition particle
    D M Freymann
    Department of Biochemistry and Biophysics, School of Medicine, University of California, San Francisco 94143 0448, USA
    Nature 385:361-4. 1997
    ..A structural subdomain unique to these evolutionarily conserved GTPases constitutes them as a distinct subfamily in the GTPase superfamily...
  14. ncbi request reprint Intracellular signaling from the endoplasmic reticulum to the nucleus: the unfolded protein response in yeast and mammals
    C Patil
    Howard Hughes Medical Institute, Department of Biochemistry and Biophysics, University of California at San Francisco, San Francisco, CA 94143, USA
    Curr Opin Cell Biol 13:349-55. 2001
    ..Recent results reveal that the output of the mammalian UPR reaches beyond transcriptional regulation of secretory pathway components to control of general translation, the cell cycle and programmed cell death...
  15. pmc A functional GTPase domain, but not its transmembrane domain, is required for function of the SRP receptor beta-subunit
    S C Ogg
    Howard Hughes Medical Institute and Department of Biochemistry and Biophysics, University of California School of Medicine, San Francisco, California 94143 0448, USA
    J Cell Biol 142:341-54. 1998
    ..This result suggests that SR functions as a regulatory switch that needs to associate with the ER membrane only transiently through interactions with other components...
  16. pmc Unraveling the interface of signal recognition particle and its receptor by using chemical cross-linking and tandem mass spectrometry
    Feixia Chu
    Mass Spectrometry Facility, University of California, San Francisco 94143 0046, USA
    Proc Natl Acad Sci U S A 101:16454-9. 2004
    ..FtsY interface in the complex...
  17. pmc Multifaceted physiological response allows yeast to adapt to the loss of the signal recognition particle-dependent protein-targeting pathway
    S C Mutka
    Howard Hughes Medical Institute and Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, California 94143 0448, USA
    Mol Biol Cell 12:577-88. 2001
    ..Thus, we suggest that cells trade speed in cell growth for fidelity in protein sorting to adjust to life without SRP...
  18. ncbi request reprint Block of HAC1 mRNA translation by long-range base pairing is released by cytoplasmic splicing upon induction of the unfolded protein response
    U Rüegsegger
    Howard Hughes Medical Institute, Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94143, USA
    Cell 107:103-14. 2001
    ..We show that the polysomal, cytoplasmic pool of HAC1 mRNA is a substrate for splicing, suggesting that the stalled ribosomes may resume translation after the intron is removed...
  19. pmc Genome-scale approaches for discovering novel nonconventional splicing substrates of the Ire1 nuclease
    Maho Niwa
    Howard Hughes Medical Institute and Department of Biochemistry and Biophysics, University of California at San Francisco, San Francisco, CA 94143 2200, USA
    Genome Biol 6:R3. 2005
    ..Because only the spliced mRNA is translated, splicing is the key regulatory step of the UPR...
  20. pmc SRP RNA controls a conformational switch regulating the SRP-SRP receptor interaction
    Saskia B Neher
    Howard Hughes Medical Institute, University of California at San Francisco, 600 16th Street, San Francisco, California 94158, USA
    Nat Struct Mol Biol 15:916-23. 2008
    ..These results demonstrate that the N-terminal helices of SRP and SR are autoinhibitory for complex formation in the absence of SRP RNA, suggesting a mechanism for RNA-mediated coordination of the SRP-SR interaction...
  21. pmc BiP binding to the ER-stress sensor Ire1 tunes the homeostatic behavior of the unfolded protein response
    David Pincus
    Department of Biochemistry and Biophysics, University of California at San Francisco, San Francisco, California, United States of America
    PLoS Biol 8:e1000415. 2010
    ..By contrast, BiP provides a buffer for inactive Ire1 molecules that ensures an appropriate response to restore protein folding homeostasis to the ER by modulating the sensitivity and dynamics of Ire1 activity...
  22. ncbi request reprint Targeting proteins to membranes: structure of the signal recognition particle
    Pascal F Egea
    Department of Biochemistry and Biophysics, University of California San Francisco, San Francisco, CA 94143, USA
    Curr Opin Struct Biol 15:213-20. 2005
    ....
  23. pmc Prm1p, a pheromone-regulated multispanning membrane protein, facilitates plasma membrane fusion during yeast mating
    M G Heiman
    Howard Hughes Medical Institute and Department of Biochemistry and Biophysics, University of California at San Francisco, San Francisco, California 94143 0448, USA
    J Cell Biol 151:719-30. 2000
    ..This phenotype suggests a role for Prm1p in plasma membrane fusion...
  24. pmc Pkh-kinases control eisosome assembly and organization
    Tobias C Walther
    Department of Biochemistry and Biophysics, Howard Hughes Medical Institute, University of California at San Francisco, San Francisco, CA, USA
    EMBO J 26:4946-55. 2007
    ..Taken together with our previous results that link eisosomes to endocytosis, these observations suggest that Pkh-kinase signaling relayed to Pil1 may help regulate endocytic events to modulate the organization of the plasma membrane...
  25. ncbi request reprint Molecular crosstalk between the nucleotide specificity determinant of the SRP GTPase and the SRP receptor
    Shu ou Shan
    Howard Hughes Medical Institute and Department of Biochemistry and Biophysics, University of California, San Francisco, California 94143 2200, USA
    Biochemistry 44:6214-22. 2005
    ..FtsY complex...
  26. pmc Signal sequences specify the targeting route to the endoplasmic reticulum membrane
    D T Ng
    Department of Biochemistry and Biophysics, University of California, School of Medicine, San Francisco 94143 0448, USA
    J Cell Biol 134:269-78. 1996
    ..Our studies show a previously unrecognized diversity in ER-directed signal sequences, that carry structural information that serves to identify the route taken...
  27. pmc Comprehensive characterization of genes required for protein folding in the endoplasmic reticulum
    Martin C Jonikas
    Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94143, USA
    Science 323:1693-7. 2009
    ..The use of a quantitative reporter in a comprehensive screen followed by systematic analysis of genetic dependencies should be broadly applicable to functional dissection of complex cellular processes from yeast to human...
  28. pmc Pil1 controls eisosome biogenesis
    Karen E Moreira
    Howard Hughes Medical Institute, and Department of Biochemistry and Biophysics, University of California at San Francisco, San Francisco, CA 94158, USA
    Mol Biol Cell 20:809-18. 2009
    ..Pil1 expression is regulated by the cell cycle, which synchronizes eisosome formation with plasma membrane growth. Our results establish a first framework of the molecular principles that define eisosome assembly and distribution...
  29. ncbi request reprint Signal sequence recognition and protein targeting
    R M Stroud
    S 960 Department of Biochemistry and Biophysics, School of Medicine, University of California, San Francisco CA 94143 0448, USA
    Curr Opin Struct Biol 9:754-9. 1999
    ..The structure of signal peptidase suggests how it releases functional proteins...
  30. ncbi request reprint Each of the activities of signal recognition particle (SRP) is contained within a distinct domain: analysis of biochemical mutants of SRP
    V Siegel
    Department of Biochemistry and Biophysics, University of California, San Francisco 94143 0448
    Cell 52:39-49. 1988
    ..This approach has allowed us to map activities to specific protein domains on SRP, and should be generally useful for analyzing other ribonucleoproteins...
  31. pmc Structure of the phylogenetically most conserved domain of SRP RNA
    U Schmitz
    Department of Pharmaceutical Chemistry, University of California, San Francisco 94143 0446, USA
    RNA 5:1419-29. 1999
    ..The structure of domain IV suggests that loop A is the initial site for the RNA/protein interaction creating specificity, whereas loop B provides a secondary interaction site...
  32. pmc Regulation of ribosome biogenesis by the rapamycin-sensitive TOR-signaling pathway in Saccharomyces cerevisiae
    T Powers
    Howard Hughes Medical Institute and Department of Biochemistry and Biophysics, University of California, School of Medicine, San Francisco, California 94143 0448, USA
    Mol Biol Cell 10:987-1000. 1999
    ..This induction has been shown previously to involve both the Ras-adenylate cyclase as well as the fermentable growth medium-induced pathways, and our results therefore suggest that these three pathways may be intimately linked...
  33. pmc Divergent effects of PERK and IRE1 signaling on cell viability
    Jonathan H Lin
    Howard Hughes Medical Institute, University of California San Francisco, San Francisco, CA, USA
    PLoS ONE 4:e4170. 2009
    ..These results demonstrate that extended PERK and IRE1 signaling have opposite effects on cell viability. Differential activation of PERK and IRE1 may determine life or death decisions after ER protein misfolding...
  34. ncbi request reprint Co-translational protein targeting by the signal recognition particle
    Shu ou Shan
    Howard Hughes Medical Institute, Department of Biochemistry and Biophysics, University of California, San Francisco, USA
    FEBS Lett 579:921-6. 2005
    ..SR complex. Each stage provides a potential control point in the targeting reaction at which regulation by additional components can be exerted, thus ensuring the binding and release of cargo at the appropriate time...
  35. pmc Mammalian endoplasmic reticulum stress sensor IRE1 signals by dynamic clustering
    Han Li
    Department of Biochemistry and Biophysics, Howard Hughes Medical Institute, University of California, San Francisco, CA 94158, USA
    Proc Natl Acad Sci U S A 107:16113-8. 2010
    ..Thus IRE1 activity is governed by a timer that may be important in switching the UPR from the initially cytoprotective phase to the apoptotic mode...
  36. ncbi request reprint tRNA ligase is required for regulated mRNA splicing in the unfolded protein response
    C Sidrauski
    Department of Biochemistry and Biophysics, University of California School of Medicine, San Francisco 94143 0448, USA
    Cell 87:405-13. 1996
    ..Our results suggest that the regulated splicing of HAC1 mRNA occurs by a novel pathway, involving tRNA ligase and bypassing the spliceosome...
  37. pmc The Golgi-resident protease Kex2 acts in conjunction with Prm1 to facilitate cell fusion during yeast mating
    Maxwell G Heiman
    Howard Hughes Medical Institute, Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94158, USA
    J Cell Biol 176:209-22. 2007
    ..Kex2 and Kex1 may promote cell fusion by proteolytically processing substrates that act in parallel to Prm1 as an alternative fusion machine, as cell wall components, or both...
  38. pmc Lipase maturation factor LMF1, membrane topology and interaction with lipase proteins in the endoplasmic reticulum
    Mark H Doolittle
    Department of Medicine, David Geffen School of Medicine, University of California, Los Angeles, California 90095, USA
    J Biol Chem 284:33623-33. 2009
    ..We discuss the implication of our results with regard to lipase maturation and DUF1222 domain structure...
  39. pmc ER membrane protein complex required for nuclear fusion
    D T Ng
    Department of Biochemistry and Biophysics, University of California Medical School, San Francisco, 94143 0448, USA
    J Cell Biol 132:499-509. 1996
    ..We propose that an ER/nuclear membrane protein complex composed of Sec63p, Sec71p, and Sec72p plays a central role in mediating nuclear membrane fusion and requires ER luminally associated Kar2p for its function...
  40. ncbi request reprint Structure of the most conserved internal loop in SRP RNA
    U Schmitz
    Department of Pharmaceutical Chemistry, University of California San Francisco, 94143 0446, USA
    Nat Struct Biol 6:634-8. 1999
    ..The structure completely explains the phylogenetic conservation of the loop bases, underlining its importance for SRP54 binding and SRP function...
  41. pmc An ER-mitochondria tethering complex revealed by a synthetic biology screen
    Benoît Kornmann
    Department of Biochemistry and Biophysics, University of California at San Francisco, San Francisco, CA 94158, USA
    Science 325:477-81. 2009
    ..The tethering complex localized to discrete foci, suggesting that discrete sites of close apposition between ER and mitochondria facilitate interorganelle calcium and phospholipid exchange...
  42. ncbi request reprint ER-phagy: selective autophagy of the endoplasmic reticulum
    Sebastián Bernales
    Howard Hughes Medical Institute, Department of Biochemistry, School of Medicine, University of California San Francisco, San Francisco, California 94158 2517, USA
    Autophagy 3:285-7. 2007
    ..ER-phagy could remove damaged or redundant parts of the ER and thus represent an important degradative functionality of the UPR that helps to afford homeostatic control...
  43. pmc The unfolded protein response signals through high-order assembly of Ire1
    Alexei V Korennykh
    Department of Biochemistry and Biophysics, University of California at San Francisco, San Francisco, California 94158, USA
    Nature 457:687-93. 2009
    ..Activation of Ire1 through oligomerization expands the mechanistic repertoire of kinase-based signalling receptors...
  44. pmc IRE1 signaling affects cell fate during the unfolded protein response
    Jonathan H Lin
    Howard Hughes Medical Institute, University of California at San Francisco, San Francisco, CA 94158, USA
    Science 318:944-9. 2007
    ..Key findings from our studies in cell culture were recapitulated in photoreceptors expressing mutant rhodopsin in animal models of retinitis pigmentosa...
  45. pmc Structures of SRP54 and SRP19, the two proteins that organize the ribonucleic core of the signal recognition particle from Pyrococcus furiosus
    Pascal F Egea
    Department of Biochemistry and Biophysics, University of California San Francisco, San Francisco, California, USA
    PLoS ONE 3:e3528. 2008
    ....
  46. pmc Structures of the signal recognition particle receptor from the archaeon Pyrococcus furiosus: implications for the targeting step at the membrane
    Pascal F Egea
    Department of Biochemistry and Biophysics, University of California San Francisco, San Francisco, California, USA
    PLoS ONE 3:e3619. 2008
    ..Based on previous structures of two sub-complexes, we propose a model of the core of archeal and eukaryotic SRP*SR targeting complexes...
  47. pmc ERMES-mediated ER-mitochondria contacts: molecular hubs for the regulation of mitochondrial biology
    Benoît Kornmann
    Department of Biochemistry and Biophysics, Howard Hughes Medical Institute, University of California at San Francisco, CA 94158, USA
    J Cell Sci 123:1389-93. 2010
    ..Finally, we discuss how cells might use ER-mitochondria communication to fine-tune these processes according to their metabolic needs...
  48. pmc Membrane expansion alleviates endoplasmic reticulum stress independently of the unfolded protein response
    Sebastian Schuck
    Howard Hughes Medical Institute and Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94158, USA
    J Cell Biol 187:525-36. 2009
    ..Thus, increasing ER size through membrane synthesis is an integral yet distinct part of the cellular program to overcome ER stress...
  49. pmc Messenger RNA targeting to endoplasmic reticulum stress signalling sites
    TOMAS ARAGON
    Department of Biochemistry and Biophysics, University of California at San Francisco, San Francisco, California 94158 2517, USA
    Nature 457:736-40. 2009
    ..Translational repression afforded by the intron fulfils this requirement for HAC1 mRNA. Recruitment of mRNA to signalling centres provides a new paradigm for the control of eukaryotic gene expression...
  50. pmc The plasma membrane proteins Prm1 and Fig1 ascertain fidelity of membrane fusion during yeast mating
    Pablo S Aguilar
    Howard Hughes Medical Institute, and Department of Biochemistry and Biophysics, University of California at San Francisco, San Francisco, CA 94158, USA
    Mol Biol Cell 18:547-56. 2007
    ..Thus, our results suggest that Prm1 and Fig1 have a role in enhancing membrane fusion and maintaining its fidelity. Their absence results in frequent mating pair lysis, which is counteracted by Ca(2+)-dependent membrane repair...
  51. pmc Endoplasmic reticulum stress in disease pathogenesis
    Jonathan H Lin
    Department of Biochemistry, University of California, San Francisco, CA 94143, USA
    Annu Rev Pathol 3:399-425. 2008
    ..This review provides background information on the unfolded protein response and discusses a selection of diseases whose pathogenesis involves ER stress...
  52. pmc Gene recruitment of the activated INO1 locus to the nuclear membrane
    Jason H Brickner
    Howard Hughes Medical Institute and the Department of Biochemistry and Biophysics, University of California, San Francisco, California, USA
    PLoS Biol 2:e342. 2004
    ..Gene recruitment to the nuclear periphery, therefore, is a dynamic process and appears to play an important regulatory role...
  53. ncbi request reprint Intracellular signaling by the unfolded protein response
    Sebastián Bernales
    Howard Hughes Medical Institute, Department of Biochemistry and Biophysics, University of California, San Francisco, California 94143, USA
    Annu Rev Cell Dev Biol 22:487-508. 2006
    ....
  54. ncbi request reprint Bypassing a kinase activity with an ATP-competitive drug
    Feroz R Papa
    Department of Medicine, University of California, San Francisco, CA 94143 2200, USA
    Science 302:1533-7. 2003
    ..Thus, rather than through phosphorylation per se, a conformational change in the kinase domain triggered by occupancy of the active site with a ligand leads to activation of all known downstream functions...
  55. ncbi request reprint Eisosomes mark static sites of endocytosis
    Tobias C Walther
    Howard Hughes Medical Institute and Department of Biochemistry and Biophysics, University of California at San Francisco, 600 16th Street, San Francisco, California 94143 2200, USA
    Nature 439:998-1003. 2006
    ..Loss of Pil1 leads to clustering of eisosome remnants and redirects endocytosis and endocytic effector proteins to these clusters...
  56. ncbi request reprint Substrate twinning activates the signal recognition particle and its receptor
    Pascal F Egea
    Department of Biochemistry and Biophysics, University of California at San Francisco, California 94143 2240, USA
    Nature 427:215-21. 2004
    ..This unique circle of twinned interactions is severed twice on hydrolysis, leading to complex dissociation after cargo delivery...
  57. pmc Autophagy counterbalances endoplasmic reticulum expansion during the unfolded protein response
    Sebastián Bernales
    Howard Hughes Medical Institute, University of California San Francisco, San Francisco, California, United States of America
    PLoS Biol 4:e423. 2006
    ..Selective ER sequestration may help cells to maintain a new steady-state level of ER abundance even in the face of continuously accumulating unfolded proteins...
  58. pmc Mechanism of association and reciprocal activation of two GTPases
    Shu ou Shan
    Department of Biochemistry and Biophysics, University of California, San Francisco, USA
    PLoS Biol 2:e320. 2004
    ..Each stage provides a potential control point in the targeting reaction at which regulation by additional components can be exerted, thus ensuring the binding and release of cargo at the appropriate time...
  59. ncbi request reprint SEC65 gene product is a subunit of the yeast signal recognition particle required for its integrity
    B C Hann
    Department of Biochemistry and Biophysics, University of California, Medical School, San Francisco 94143 0448
    Nature 356:532-3. 1992
    ..cerevisiae SRP and that it is required for the stable association of another subunit, SRP54p, with SRP. Overexpression of SRP54p suppresses both growth and protein translocation defects in sec65-1 mutant cells...
  60. ncbi request reprint Reciprocal stimulation of GTP hydrolysis by two directly interacting GTPases
    T Powers
    Department of Biochemistry and Biophysics, University of California Medical School, San Francisco 94143 0448, USA
    Science 269:1422-4. 1995
    ..5S ribonucleoprotein and GTP. Thus, nucleotide triphosphate hydrolysis by Ffh and FtsY is likely to occur in reciprocally coupled reactions in which the two interacting guanosine triphosphatases act as regulatory proteins for each other...
  61. pmc Membrane lysis during biological membrane fusion: collateral damage by misregulated fusion machines
    Alex Engel
    Howard Hughes Medical Institute and Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94158, USA
    J Cell Biol 183:181-6. 2008
    ..The convergence of these findings showing membrane integrity loss during biological membrane fusion suggests new mechanistic models for membrane fusion and the role of membrane fusion complexes...
  62. pmc Signal recognition particle receptor is important for cell growth and protein secretion in Saccharomyces cerevisiae
    S C Ogg
    Department of Biochemistry and Biophysics, University of California, Medical School, San Francisco 94143 0448
    Mol Biol Cell 3:895-911. 1992
    ..This adaptation is a physiological process and is not due to the accumulation of a suppressor mutation. The degree of this adaptation is strain dependent...
  63. ncbi request reprint The signal recognition particle in S. cerevisiae
    B C Hann
    Department of Biochemistry and Biophysics, University of California, Medical School, San Francisco 94143 0448
    Cell 67:131-44. 1991
    ..The ability of some proteins to be efficiently targeted in SRP-deficient cells may explain why previous genetic and biochemical analyses in yeast and bacteria did not reveal components of the SRP-dependent protein targeting pathway...
  64. pmc Structure of sterol aliphatic chains affects yeast cell shape and cell fusion during mating
    Pablo S Aguilar
    Departments of Biochemistry and Biophysics, Howard Hughes Medical Institute, University of California, San Francisco, CA 94158, USA
    Proc Natl Acad Sci U S A 107:4170-5. 2010
    ..Thus, a two-atom change in the aliphatic moiety of ergosterol is sufficient to obstruct cell shape remodeling and cell fusion...
  65. pmc Identification of yeast proteins necessary for cell-surface function of a potassium channel
    Friederike A Haass
    Department of Physiology, Neuroscience Graduate Program, and Howard Hughes Medical Institute, University of California, San Francisco, CA 94158, USA
    Proc Natl Acad Sci U S A 104:18079-84. 2007
    ..The seven yeast proteins identified in our screen likely impact Kir-channel functional expression at the level of vesicle budding from the ER and/or the local lipid environment at the plasma membrane...
  66. pmc Functional genomic screen reveals genes involved in lipid-droplet formation and utilization
    Yi Guo
    Department of Biochemistry and Biophysics, University of California, San Francisco, California 94158, USA
    Nature 453:657-61. 2008
    ..These phenotypes are conserved in mammalian cells, suggesting that insights from these studies are likely to be central to our understanding of human diseases involving excessive lipid storage...
  67. ncbi request reprint Structural basis of FFAT motif-mediated ER targeting
    Stephen E Kaiser
    Department of Molecular and Cellular Physiology, Stanford University, Stanford, CA 94305, USA
    Structure 13:1035-45. 2005
    ..Our data establish the structural basis of FFAT-mediated ER targeting and suggest that FFAT-targeted proteins play an important role in determining ER morphology...
  68. pmc Gcn4p and novel upstream activating sequences regulate targets of the unfolded protein response
    Christopher K Patil
    Howard Hughes Medical Institute, Chevy Chase, Maryland, USA
    PLoS Biol 2:E246. 2004
    ..The discovery of a role for Gcn4p in the yeast UPR reveals an additional level of complexity and demonstrates a surprising conservation of the signaling circuit between yeast and metazoan cells...
  69. pmc Structural basis for mobility in the 1.1 A crystal structure of the NG domain of Thermus aquaticus Ffh
    Ursula D Ramirez
    Department of Molecular Pharmacology and Biological Chemistry, Northwestern University Medical School, 303 E Chicago Avenue, MC S215, Chicago, IL 60611 3008, USA
    J Mol Biol 320:783-99. 2002
    ..Our data allows us to propose a structural explanation for the functional significance of sequence elements conserved at the N/G interface...
  70. pmc Profile of Peter Walter
    Tinsley H Davis
    Proc Natl Acad Sci U S A 103:5259-61. 2006
  71. doi request reprint Effects of bevacizumab (Avastin) on retinal cells in organotypic culture
    Stefanie Kaempf
    Department of Ophthalmology and IZKF BIOMAT, RWTH Aachen University, Aachen, Germany
    Invest Ophthalmol Vis Sci 49:3164-71. 2008
    ..The aim of this study was to evaluate the toxicity of bevacizumab in the adult mammalian neurosensory retina in culture...
  72. ncbi request reprint The strange case of the signal recognition particle
    Karl S Matlin
    University of Cincinnati College of Medicine, The Vontz Center, ML 0581, 3125 Eden Avenue, Cincinnati, Ohio 45219 0581, USA
    Nat Rev Mol Cell Biol 3:538-42. 2002
  73. ncbi request reprint Translocation of lipid-linked oligosaccharides across the ER membrane requires Rft1 protein
    Jonne Helenius
    Institute of Microbiology, Swiss Federal Institute of Technology, Zurich, CH 8092 Zurich, Switzerland
    Nature 415:447-50. 2002
    ..Here we provide evidence that yeast RFT1 encodes an evolutionarily conserved protein required for the translocation of Man5GlcNAc2-PP-Dol from the cytoplasmic to the lumenal leaflet of the ER membrane...
  74. pmc Conformational changes in the GTPase modules of the signal reception particle and its receptor drive initiation of protein translocation
    Shu ou Shan
    Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA 91125, USA
    J Cell Biol 178:611-20. 2007
    ..Most importantly, our results show that an elaborate rearrangement within the SRP-SR GTPase complex is required to drive the unloading and initiate translocation of cargo proteins...
  75. ncbi request reprint Amplitude calculation in multifocal ERG: comparison of repeatability in 30 Hz flicker and first order kernel stimulation
    Babac A E Mazinani
    Department of Ophthalmology, RWTH Aachen University, Pauwelsstrasse 30, 52057, Aachen, Germany
    Graefes Arch Clin Exp Ophthalmol 245:338-44. 2007
    ..With this method, amplitudes can be calculated without having to localise peaks and troughs and set cursors. The purpose of this study is to compare the re-test stability of this method to conventional mfERG stimulation...
  76. ncbi request reprint Signal integration in the endoplasmic reticulum unfolded protein response
    David Ron
    The Kimmel Center for Biology and Medicine at the Skirball Institute, New York University School of Medicine, 540 First Avenue, New York, New York 10016, USA
    Nat Rev Mol Cell Biol 8:519-29. 2007
    ..The arms of the UPR are integrated to provide a response that remodels the secretory apparatus and aligns cellular physiology to the demands imposed by ER stress...
  77. ncbi request reprint Ocular aberrations in barn owl eyes
    Wolf M Harmening
    Department of Zoology and Animal Physiology, RWTH Aachen University, Kopernikusstrasse 16, 52056, Aachen, Germany
    Vision Res 47:2934-42. 2007
    ..Total RMS wavefront error was between 0.06 and 0.15 microm (mean: 0.10 microm, STD: 0.03 microm, defocus cancelled) for a 6 mm pupil. The results suggest that image quality in barn owl eyes is excellent...
  78. ncbi request reprint Different states of energy metabolism in the vertebrate retina can be identified by stimulus-related changes in near UV transmission
    Peter Walter
    Department of Ophthalmology, RWTH Aachen University, Pauwelsstr 30, 52074, Aachen, Germany
    Graefes Arch Clin Exp Ophthalmol 245:547-54. 2007
    ....
  79. ncbi request reprint The effect of membrane differential filtration on the colloid osmotic pressure in patients with age-related macular degeneration: significance to visual function?
    Christoph Lüke
    Center of Ophthalmology, Department of Vitreoretinal Surgery, University of Koln, Koln, Germany
    Ther Apher Dial 7:263-8. 2003
    ..Based on the observation that visual field and COP are correlated, one may hope for a means to improve decreased central retinal function in eyes with geographic AMD by modulation of COP plasma level...
  80. ncbi request reprint Visual resolution with retinal implants estimated from recordings in cat visual cortex
    Reinhard Eckhorn
    Department of Physics, Neurophysics Group, Philipps University Marburg, and University Eye Hospital, Medical Faculty University of Tübingen, Germany
    Vision Res 46:2675-90. 2006
    ..8 cm at about 1m and correspondingly 8 cm at 10 m distance seems sufficient for useful object recognition and visuo-motor behavior in many in- and out-door situations of daily life...
  81. ncbi request reprint Cortical activation via an implanted wireless retinal prosthesis
    Peter Walter
    Department of Ophthalmology, RWTH Aachen University, Pauwelsstrasse 30, 52074 Aachen, Germany
    Invest Ophthalmol Vis Sci 46:1780-5. 2005
    ..To demonstrate local cortical activations in the primary visual cortex of the cat as a result of retinal electrical stimulation by means of a completely wireless-controlled, implantable retinal prosthesis in a series of acute experiments...
  82. doi request reprint Novel organotypic culture model of adult mammalian neurosensory retina in co-culture with retinal pigment epithelium
    Stefanie Kaempf
    Department of Ophthalmology and IZKF BIOMAT, RWTH Aachen University, Pauwelsstr 30, 52074 Aachen, Germany
    J Neurosci Methods 173:47-58. 2008
    ..The purpose of this study was to assess survival of adult mammalian neurosensory retina cultured in contact with the layer of a choroid-retinal pigment epithelium (RPE) explant...
  83. ncbi request reprint A method and technical equipment for an acute human trial to evaluate retinal implant technology
    Ralf Hornig
    IIP Technologies GmbH, Bonn, Germany
    J Neural Eng 2:S129-34. 2005
    ....