Research Topics
Genomes and Genes
| Kay Uwe WagnerSummaryAffiliation: University of Nebraska Medical Center Country: USA Publications
Research Grants
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Detail Information
Publications
Conditional deletion of the Bcl-x gene from erythroid cells results in hemolytic anemia and profound splenomegalyK U Wagner
Laboratory of Genetics and Physiology, National Institute of Diabetes, Digestive, and Kidney Diseases, National Institutes of Health, Bldg 8, Rm 107, Bethesda, MD 20892 0822, USA
Development 127:4949-58. 2000..Mice conditionally deficient in Bcl-x permitted us for the first time to study the effects of Bcl-x deficiency on cell proliferation, maturation and survival under physiological conditions in an adult animal...
Impaired alveologenesis and maintenance of secretory mammary epithelial cells in Jak2 conditional knockout miceKay Uwe Wagner
Eppley Institute for Research in Cancer and Allied Diseases and Department of Pathology, University of Nebraska Medical Center, Omaha, 68198 6805, USA
Mol Cell Biol 24:5510-20. 2004....- Pregnancy and stem cell behaviorKay Uwe Wagner
Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Nebraska Medical Center, Omaha, Nebraska 68198, USA
J Mammary Gland Biol Neoplasia 10:25-36. 2005..Using appropriate animal models, we further demonstrate that PI-MECs are cellular targets for pregnancy-enhanced mammary tumorigenesis... 
Models of breast cancer: quo vadis, animal modeling?Kay Uwe Wagner
University of Nebraska Medical Center, Omaha, Nebraska, USA
Breast Cancer Res 6:31-8. 2004....- Tsg101 is essential for cell growth, proliferation, and cell survival of embryonic and adult tissuesKay Uwe Wagner
Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha 68198 6805, USA
Mol Cell Biol 23:150-62. 2003..In summary, our results suggest that Tsg101 is required for normal cell function of embryonic and adult tissues but that this gene is not a tumor suppressor for sporadic forms of breast cancer... 
Jak2/Stat5 signaling in mammogenesis, breast cancer initiation and progressionKay Uwe Wagner
Eppley Institute for Research in Cancer and Allied Diseases, Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NE 68198, USA
J Mammary Gland Biol Neoplasia 13:93-103. 2008..Future studies to further test the concept of contrasting effects of Jak2/Stat5 pathway on breast cancer initiation and metastatic progression are proposed...- Spatial and temporal expression of the Cre gene under the control of the MMTV-LTR in different lines of transgenic miceK U Wagner
Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha 68198 6805, USA
Transgenic Res 10:545-53. 2001..Information provided by this study will guide the researcher to those cell types and developmental stages at which a phenotype can be expected upon deletion of relevant genes... - An adjunct mammary epithelial cell population in parous females: its role in functional adaptation and tissue renewalKay Uwe Wagner
Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, 986805 Nebraska Medical Center, Rm 8009, Omaha, NE 68198 6805, USA
Development 129:1377-86. 2002..e. ductal morphogenesis and lobulogenesis). We propose that this parity-induced population contributes importantly to the biological differences between the mammary glands of parous and nulliparous females... - Bcl-x is not required for maintenance of follicles and corpus luteum in the postnatal mouse ovaryGregory Riedlinger
Laboratory of Genetics and Physiology, National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
Biol Reprod 66:438-44. 2002..Whereas luteal cells underwent apoptosis in the absence of the PrlR, no changes were observed in the absence of Stat5 or Bcl-x... - Cell cycle arrest and cell death are controlled by p53-dependent and p53-independent mechanisms in Tsg101-deficient cellsMarissa J Carstens
Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Nebraska Medical Center, Omaha, Nebraska 68198 6805, USA
J Biol Chem 279:35984-94. 2004.... - Basal activation of transcription factor signal transducer and activator of transcription (Stat5) in nonpregnant mouse and human breast epitheliumMarja T Nevalainen
United States Military Cancer Institute and Department of Pathology, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20852, USA
Mol Endocrinol 16:1108-24. 2002..Continuous activation of Stat5 indicates a role of this transcription factor in normal, nonpregnant breast epithelial cells, and may shed new light on Stat5 involvement in breast tumor promotion... - Stat5 promotes survival of mammary epithelial cells through transcriptional activation of a distinct promoter in Akt1Bradley A Creamer
Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, 985950 Nebraska Medical Center, Omaha, NE 68198 5950, USA
Mol Cell Biol 30:2957-70. 2010..Collectively, the results of the present study elucidate an underlying mechanism by which active Stat5 mediates evasion from apoptosis and self-sufficiency in growth signals... - Targeted deletion of the Tsg101 gene results in cell cycle arrest at G1/S and p53-independent cell deathAndrea Krempler
Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Nebraska Medical Center, Omaha, Nebraska 68198 6805, USA
J Biol Chem 277:43216-23. 2002..Our results clearly demonstrate that Tsg101 is not a primary tumor suppressor in mouse embryonic fibroblasts. However, the protein is crucial for cell proliferation and cell survival... - Activation of beta-catenin in prostate epithelium induces hyperplasias and squamous transdifferentiationBrian Bierie
Laboratory of Genetics and Physiology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Oncogene 22:3875-87. 2003..This suggests that the transdifferentiation into squamous metaplasias is an early response of endoderm-derived cells to beta-catenin, and that the development of intra-acinous hyperplasias or neoplastic foci is a later event... - Expression of the whey acidic protein (Wap) is necessary for adequate nourishment of the offspring but not functional differentiation of mammary epithelial cellsAleata A Triplett
Eppley Institute for Research in Cancer and Allied Diseases and the Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, Nebraska 68198 6805, USA
Genesis 43:1-11. 2005..Important implications of these findings for the use of Wap as a marker for advanced differentiation of mammary epithelial cells and the biology of pluripotent progenitors are discussed in the final section... - Generation of a conditional knockout allele for the Janus kinase 2 (Jak2) gene in miceAndrea Krempler
Eppley Institute for Research in Cancer and Allied Diseases and the Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, Nebraska 68198 6805, USA
Genesis 40:52-7. 2004.... - Parity-induced mammary epithelial cells facilitate tumorigenesis in MMTV-neu transgenic miceMaLinda D Henry
Eppley Institute for Research in Cancer and Allied Diseases and the Department of Pathology and Microbiology, University of Nebraska Medical Center, 986805 Nebraska Medical Center, Omaha, NE 68198 6805, USA
Oncogene 23:6980-5. 2004.... - Temporally and spatially controlled expression of transgenes in embryonic and adult tissuesQian Zhang
Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, 985950 Nebraska Medical Center, DRCII, Rm 5033, Omaha, NE 68198 5950, USA
Transgenic Res 19:499-509. 2010.... - A knockout of the Tsg101 gene leads to decreased expression of ErbB receptor tyrosine kinases and induction of autophagy prior to cell deathChantey R Morris
Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, Nebraska, United States of America
PLoS ONE 7:e34308. 2012..These multifaceted alterations need to be recognized as they have an impact on defining particular functions for Tsg101 in processes such as signal transduction and lysosomal/endosomal trafficking... - Cyclin D3 compensates for the loss of cyclin D1 during ErbB2-induced mammary tumor initiation and progressionQian Zhang
Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, Nebraska 68198, USA
Cancer Res 71:7513-24. 2011..Collectively, the results of this study suggest that only the combined inhibition of Cyclin D1 and D3 might be a suitable strategy for breast cancer prevention and therapy... - Parity-induced mammary epithelial cells are multipotent and express cell surface markers associated with stem cellsLaurice A Matulka
Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, 986805 Nebraska Medical Center, Rm 8009, Omaha, NE 68198 6805, USA
Dev Biol 303:29-44. 2007..These observations suggest that PI-MECs contain multipotent progenitors that are able to self renew and generate diverse epithelial lineages present in the murine mammary gland... - Longitudinal analysis of mammogenesis using a novel tetracycline-inducible mouse model and in vivo imagingBradley A Creamer
Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, 986805 Nebraska Medical Center, Omaha, Nebraska 68198 6805, USA
Genesis 47:234-45. 2009..The study shows that, unlike their progeny in mammary epithelial transplants as reported previously, PI-MECs themselves may not belong to the long-term label-retaining epithelial subtype... - The Janus kinase 2 is required for expression and nuclear accumulation of cyclin D1 in proliferating mammary epithelial cellsKazuhito Sakamoto
Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, 986805 Nebraska Medical Center, Omaha, Nebraska 68198 6805, USA
Mol Endocrinol 21:1877-92. 2007.... - Loss of the peroxisome proliferation-activated receptor gamma (PPARgamma ) does not affect mammary development and propensity for tumor formation but leads to reduced fertilityYongzhi Cui
Laboratory of Genetics and Physiology, NIDDK, the Laboratory of Immunoregulation, Immune Activation Section, NIAID, National Institutes of Health, Bethesda, Maryland 20892, USA
J Biol Chem 277:17830-5. 2002..These mice have normal populations of follicles, they ovulate and develop corpora lutea. Although progesterone levels are decreased and implantation rates are reduced, the exact cause of the impaired fertility remains to be determined... - Thymic stromal lymphopoietin-mediated STAT5 phosphorylation via kinases JAK1 and JAK2 reveals a key difference from IL-7-induced signalingYrina Rochman
Laboratory of Molecular Immunology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892 1674, USA
Proc Natl Acad Sci U S A 107:19455-60. 2010..These findings clarify the basis for TSLP-mediated signaling and provide an example wherein a cytokine uses JAK1 and JAK2 to mediate the activation of STAT5... - Targeting janus kinase 2 in Her2/neu-expressing mammary cancer: Implications for cancer prevention and therapyKazuhito Sakamoto
Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, 68198 5950, USA
Cancer Res 69:6642-50. 2009..This might be a compensatory mechanism that explains why Jak2 is a relevant target for preventing the initiation but not the progression of ErbB2-associated mammary cancer... - Brca1-deficient murine mammary epithelial cells have increased sensitivity to CDDP and MMSMagdalene K Sgagias
Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, Nebraska 68198 6805, USA
Cell Cycle 3:1451-6. 2004..These studies support the role of BRCA1 in the CDDP-induced and MMS-induced DNA damage and repair by p53-independent pathways... - Parity-induced mouse mammary epithelial cells are pluripotent, self-renewing and sensitive to TGF-beta1 expressionCorinne A Boulanger
Mammary Biology and Tumorigenesis Laboratory, National Cancer Institute, Bethesda, MD 20892, USA
Oncogene 24:552-60. 2005.... - A mammary-specific, long-range deletion on mouse chromosome 11 accelerates Brca1-associated mammary tumorigenesisAleata A Triplett
Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, NE 68198 6805, USA
Neoplasia 10:1325-34. 2008..The analysis of the coding sequence and expression pattern of p53 and p21 suggests that loss-of-heterozygosity of Trp53 caused by somatic mutations contributes to accelerated mammary tumorigenesis in this model... - Loss of Dnmt3b function upregulates the tumor modifier Ment and accelerates mouse lymphomagenesisRyan A Hlady
Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, Nebraska 68198 5950, USA
J Clin Invest 122:163-77. 2012..Our findings identify Ment as an enhancer of lymphomagenesis that contributes to the tumor suppressor function of Dnmt3b and suggest it could be a potential target for anticancer therapies... - Generation of a novel MMTV-tTA transgenic mouse strain for the targeted expression of genes in the embryonic and postnatal mammary glandKazuhito Sakamoto
Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, Nebraska, United States of America
PLoS ONE 7:e43778. 2012.... - Endosomal-sorting complexes required for transport (ESCRT) pathway-dependent endosomal traffic regulates the localization of active Src at focal adhesionsChun Tu
Eppley Institute for Research in Cancer and Allied Diseases and University of Nebraska Medical Center Eppley Cancer Center, University of Nebraska Medical Center, Omaha, NE 68198, USA
Proc Natl Acad Sci U S A 107:16107-12. 2010..These results reveal a critical role for an ESCRT pathway-dependent LE/LY trafficking step in Src function by promoting localization of active Src to FAs... - IRF4 is a suppressor of c-Myc induced B cell leukemiaSimanta Pathak
Department of Genetics, Cell Biology and Anatomy, University of Nebraska Medical Center, Omaha, Nebraska, United States of America
PLoS ONE 6:e22628. 2011..Thus, IRF4 functions as a classical tumor suppressor to inhibit c-Myc induced B cell leukemia in EμMyc mice... - The two faces of Janus kinases and their respective STATs in mammary gland development and cancerKay Uwe Wagner
Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, 985950 Nebraska Medical Center, DRC2, Rm 5033, Omaha, NE, USA
J Carcinog 10:32. 2011.... - Transforming growth factor-beta regulates mammary carcinoma cell survival and interaction with the adjacent microenvironmentBrian Bierie
Department of Cancer Biology, Vanderbilt University, Nashville, TN 37232 6838, USA
Cancer Res 68:1809-19. 2008.... - Early onset of neoplasia in the prostate and skin of mice with tissue-specific deletion of PtenStephanie A Backman
Department of Medical Biophysics, University of Toronto, Ontario Cancer Institute and University Health Network, 610 University Avenue, Toronto, Ontario, Canada M5G 2M9
Proc Natl Acad Sci U S A 101:1725-30. 2004..Consistent with high PTEN mutation rates in human prostate tumors, these data indicate that PTEN is a critical tumor suppressor in this organ... - Loss of the LIM domain protein Lmo4 in the mammary gland during pregnancy impedes lobuloalveolar developmentEleanor Y M Sum
The Walter and Eliza Hall Institute of Medical Research and Bone Marrow Research Laboratories, 1G Royal Parade, Parkville, VIC 3050, Australia
Oncogene 24:4820-8. 2005..Thus, the use of a conditional targeting strategy has revealed that Lmo4 is required for proper development of the mammary gland during pregnancy and indicated that Lmo4 acts as a positive regulator of alveolar epithelial proliferation... - Impaired differentiation and lactational failure of Erbb4-deficient mammary glands identify ERBB4 as an obligate mediator of STAT5Weiwen Long
Department of Structural and Cellular Biology, Tulane Cancer Center, Tulane University Health Sciences Center, 1430 Tulane Avenue, New Orleans, Louisiana 70112 2699, USA
Development 130:5257-68. 2003.... - HIF1alpha is a critical regulator of secretory differentiation and activation, but not vascular expansion, in the mouse mammary glandTiffany N Seagroves
Division of Biological Sciences, Molecular Biology Section, University of California San Diego, La Jolla, CA 92093, USA
Development 130:1713-24. 2003..Transplantation experiments confirmed that these developmental defects were mammary epithelial cell autonomous. These data make clear that HIF1alpha plays a critical role in the differentiation and function of the mammary epithelium... - Epithelial-specific and stage-specific functions of insulin-like growth factor-I during postnatal mammary developmentAimee V Loladze
Department of Neural and Behavioral Sciences, Pennsylvania State University College of Medicine, Hershey, Pennsylvania 17033, USA
Endocrinology 147:5412-23. 2006..Taken together, our results support distinct roles for IGF-I expressed in epithelial and stromal compartments in mediating growth of the postnatal mammary gland... - Coactivation of janus tyrosine kinase (Jak)1 positively modulates prolactin-Jak2 signaling in breast cancer: recruitment of ERK and signal transducer and activator of transcription (Stat)3 and enhancement of Akt and Stat5a/b pathwaysLynn M Neilson
Kimmel Cancer Center, Department of Cancer Biology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
Mol Endocrinol 21:2218-32. 2007.... - Estrogen receptor-alpha expression in the mammary epithelium is required for ductal and alveolar morphogenesis in miceYuxin Feng
Department of Cell and Cancer Biology, Vontz Center for Molecular Studies, University of Cincinnati College of Medicine, Cincinnati, OH 45267, USA
Proc Natl Acad Sci U S A 104:14718-23. 2007..These observations suggest that ERalpha expression in the mammary epithelium is essential for normal ductal morphogenesis during puberty and alveologenesis during pregnancy and lactation... - Conditional loss of PTEN leads to precocious development and neoplasia in the mammary glandGang Li
Department of Molecular and Medical Pharmacology, UCLA School of Medicine, 650 Circle Drive South, 90095 1735, USA
Development 129:4159-70. 2002.... - Brca2 deficiency does not impair mammary epithelium development but promotes mammary adenocarcinoma formation in p53(+/-) mutant miceAlison M Y Cheung
Advanced Medical Discovery Institute, University Health Network, 620 University Avenue, Suite 706, Toronto, Ontario M5G 2C1, Canada
Cancer Res 64:1959-65. 2004..Our data indicate that Brca2 is not essential for mammary epithelium development but that Brca2 deficiency and down-regulated p53 expression can work jointly to promote mammary tumorigenesis...
Research Grants
- Tsg101 - a Modulator of ErbB2 Signaling in Breast CancerKay Uwe Wagner; Fiscal Year: 2010..The genetic and molecular studies outlined in this application will help to predict the potential value of Tsg101 as a therapeutic target before a drug will be developed and tested in preclinical trials. ..
- Growth-Regulatory Signaling Networks in Breast CancerKay Uwe Wagner; Fiscal Year: 2010..Furthermore, they might reveal whether a combinatorial therapy of pan-ErbB tyrosine kinase inhibitors and Jak2 inhibitors would be beneficial for the treatment of ErbB2-positive breast cancers. ..
- Growth-Regulatory Signaling Networks in Breast CancerKay Uwe Wagner; Fiscal Year: 2007..Furthermore, they might reveal whether a combinatorial therapy of pan-ErbB tyrosine kinase inhibitors and Jak2 inhibitors would be beneficial for the treatment of ErbB2-positive breast cancers. ..
- Tumor Susceptibility Gene 101 Deficiency and NeoplasiaKay Uwe Wagner; Fiscal Year: 2006..abstract_text> ..
- Tsg101 - a Modulator of ErbB2 Signaling in Breast CancerKay Uwe Wagner; Fiscal Year: 2009..The genetic and molecular studies outlined in this application will help to predict the potential value of Tsg101 as a therapeutic target before a drug will be developed and tested in preclinical trials. ..