P Wadsworth

Summary

Affiliation: University of Massachusetts
Country: USA

Publications

  1. pmc Centrosome fragments and microtubules are transported asymmetrically away from division plane in anaphase
    Nasser M Rusan
    Department of Biology and Program in Molecular and Cellular Biology, University of Massachusetts, Amherst, MA 01003, USA
    J Cell Biol 168:21-8. 2005
  2. pmc Reorganization of the microtubule array in prophase/prometaphase requires cytoplasmic dynein-dependent microtubule transport
    Nasser M Rusan
    Department of Biology, Program in Molecular and Cellular Biology, Morrill Science Center, University of Massachusetts, N Pleasant Street, Amherst, MA 01003, USA
    J Cell Biol 158:997-1003. 2002
  3. pmc TPX2 regulates the localization and activity of Eg5 in the mammalian mitotic spindle
    Nan Ma
    Department of Biology, University of Massachusetts, Amherst, MA 01003, USA
    J Cell Biol 195:87-98. 2011
  4. doi request reprint Microtubule motors: doin' it without dynactin
    Patricia Wadsworth
    Department of Biology and Program in Molecular and Cellular Biology, University of Massachusetts Amherst, Amherst, MA 01003, USA
    Curr Biol 23:R563-5. 2013
  5. doi request reprint Variations on theme: spindle assembly in diverse cells
    Patricia Wadsworth
    Department of Biology, Morrill Science Center, University of Massachusetts, Amherst, MA 01003, USA
    Protoplasma 248:439-46. 2011
  6. ncbi request reprint Stable expression of fluorescently tagged proteins for studies of mitosis in mammalian cells
    Patricia Wadsworth
    Department of Biology, University of Massachusetts, Amherst 01003, USA
    Nat Methods 2:981-7. 2005
  7. ncbi request reprint Cell biology. Persistence pays
    Patricia Wadsworth
    Department of Biology, University of Massachusetts, Amherst, MA 01003, USA
    Science 300:1675-7. 2003
  8. ncbi request reprint Regional regulation of microtubule dynamics in polarized, motile cells
    P Wadsworth
    Department of Biology and Program in Molecular and Cellular Biology, University of Massachusetts, Amherst 01003, USA
    Cell Motil Cytoskeleton 42:48-59. 1999
  9. pmc Cell cycle-dependent changes in microtubule dynamics in living cells expressing green fluorescent protein-alpha tubulin
    N M Rusan
    Department of Biology and Program in Molecular and Cellular Biology, University of Massachusetts, Amherst 01003, USA
    Mol Biol Cell 12:971-80. 2001
  10. ncbi request reprint Centrosome behavior in motile HGF-treated PtK2 cells expressing GFP-gamma tubulin
    B A Danowski
    Department of Biology, Union College, Schnectady, New York 12308, USA
    Cell Motil Cytoskeleton 50:59-68. 2001

Research Grants

  1. Purchase of Leica TCS SP2 Confocal Microscope
    Patricia Wadsworth; Fiscal Year: 2002
  2. Mechanisms of Microtubule Dynamic Turnover
    Patricia Wadsworth; Fiscal Year: 2009
  3. MECHANISM AND REGULATION OF MICROTUBULE TURNOVER
    Patricia Wadsworth; Fiscal Year: 2003

Collaborators

Detail Information

Publications28

  1. pmc Centrosome fragments and microtubules are transported asymmetrically away from division plane in anaphase
    Nasser M Rusan
    Department of Biology and Program in Molecular and Cellular Biology, University of Massachusetts, Amherst, MA 01003, USA
    J Cell Biol 168:21-8. 2005
    ..Biased MT release may provide a mechanism for MT-dependent positioning of components necessary for specifying the site of contractile ring formation...
  2. pmc Reorganization of the microtubule array in prophase/prometaphase requires cytoplasmic dynein-dependent microtubule transport
    Nasser M Rusan
    Department of Biology, Program in Molecular and Cellular Biology, Morrill Science Center, University of Massachusetts, N Pleasant Street, Amherst, MA 01003, USA
    J Cell Biol 158:997-1003. 2002
    ..The data demonstrate that dynamic astral Mts search the cytoplasm for other Mts, as well as chromosomes, in mitotic cells...
  3. pmc TPX2 regulates the localization and activity of Eg5 in the mammalian mitotic spindle
    Nan Ma
    Department of Biology, University of Massachusetts, Amherst, MA 01003, USA
    J Cell Biol 195:87-98. 2011
    ..These results establish a novel function of TPX2 in regulating the location and activity of the mitotic motor Eg5...
  4. doi request reprint Microtubule motors: doin' it without dynactin
    Patricia Wadsworth
    Department of Biology and Program in Molecular and Cellular Biology, University of Massachusetts Amherst, Amherst, MA 01003, USA
    Curr Biol 23:R563-5. 2013
    ..New research now shows that dynein accomplishes many of its mitotic functions without dynactin. ..
  5. doi request reprint Variations on theme: spindle assembly in diverse cells
    Patricia Wadsworth
    Department of Biology, Morrill Science Center, University of Massachusetts, Amherst, MA 01003, USA
    Protoplasma 248:439-46. 2011
    ..We hypothesize that the essential process for spindle assembly is the motor-driven organization of microtubules that accumulate in the form of dense bundles at or near the chromosomes...
  6. ncbi request reprint Stable expression of fluorescently tagged proteins for studies of mitosis in mammalian cells
    Patricia Wadsworth
    Department of Biology, University of Massachusetts, Amherst 01003, USA
    Nat Methods 2:981-7. 2005
  7. ncbi request reprint Cell biology. Persistence pays
    Patricia Wadsworth
    Department of Biology, University of Massachusetts, Amherst, MA 01003, USA
    Science 300:1675-7. 2003
  8. ncbi request reprint Regional regulation of microtubule dynamics in polarized, motile cells
    P Wadsworth
    Department of Biology and Program in Molecular and Cellular Biology, University of Massachusetts, Amherst 01003, USA
    Cell Motil Cytoskeleton 42:48-59. 1999
    ..The data indicate that region specific differences in the organization and dynamics of actin filaments may regulate microtubule dynamic instability behavior in vivo...
  9. pmc Cell cycle-dependent changes in microtubule dynamics in living cells expressing green fluorescent protein-alpha tubulin
    N M Rusan
    Department of Biology and Program in Molecular and Cellular Biology, University of Massachusetts, Amherst 01003, USA
    Mol Biol Cell 12:971-80. 2001
    ..Microtubule release from the centrosome and a subset of differentially stable astral microtubules were also observed. The results provide the first quantitative measurements of mitotic microtubule dynamics in mammalian cells...
  10. ncbi request reprint Centrosome behavior in motile HGF-treated PtK2 cells expressing GFP-gamma tubulin
    B A Danowski
    Department of Biology, Union College, Schnectady, New York 12308, USA
    Cell Motil Cytoskeleton 50:59-68. 2001
    ..Because microtubules are required for efficient cellular locomotion, we propose that non-centrosomal microtubules stabilize the direction of locomotion without a requirement for reorientation of the centrosome...
  11. ncbi request reprint Peripheral, non-centrosome-associated microtubules contribute to spindle formation in centrosome-containing cells
    U S Tulu
    Department of Biology and Program in Molecular and Cellular Biology, University of Massachusetts, Amherst 01003, USA
    Curr Biol 13:1894-9. 2003
    ..Our results directly demonstrate that non-centrosome-associated microtubules contribute to spindle formation in centrosome-containing cells...
  12. pmc Antagonistic forces generated by myosin II and cytoplasmic dynein regulate microtubule turnover, movement, and organization in interphase cells
    A M Yvon
    Program in Molecular and Cellular Biology and Department of Biology, University of Massachusetts, Amherst, MA 01003, USA
    Proc Natl Acad Sci U S A 98:8656-61. 2001
    ..These results demonstrate that myosin II and cytoplasmic dynein contribute to a balance of forces that regulates MT organization, movement, and turnover in interphase cells...
  13. ncbi request reprint Quantification of microtubule nucleation, growth and dynamics in wound-edge cells
    Kimberly J Salaycik
    Department of Biology, Program in Molecular and Cellular Biology, University of Massachusetts, Amherst, MA 01003, USA
    J Cell Sci 118:4113-22. 2005
    ....
  14. doi request reprint Photoactivatable green fluorescent protein-tubulin
    U Serdar Tulu
    Department of Biology, Duke University, Durham, North Carolina 27708, USA
    Methods Cell Biol 97:81-90. 2010
    ..Use of photoactivatable proteins is a powerful approach to examine dynamic processes, including spindle formation, in diverse cells...
  15. ncbi request reprint A conserved role for kinesin-5 in plant mitosis
    Alex Bannigan
    Biology Department, University of Massachusetts, Amherst, MA 01003, USA
    J Cell Sci 120:2819-27. 2007
    ..These results are among the first reports of any motor with a major role in anastral spindle structure in plants and demonstrate that the conservation of kinesin-5 motor function throughout eukaryotes extends to vascular plants...
  16. pmc Centrosome reorientation in wound-edge cells is cell type specific
    Anne Marie C Yvon
    Department of Biology and Program in Molecular and Cellular Biology, University of Massachusetts, Amherst, MA 01002, USA
    Mol Biol Cell 13:1871-80. 2002
    ..Our data demonstrate that centrosome reorientation is not required for directed migration and that diverse cells use distinct mechanisms for remodeling the microtubule array during directed migration...
  17. pmc Molecular requirements for kinetochore-associated microtubule formation in mammalian cells
    U Serdar Tulu
    Department of Biology and Program in Molecular and Cellular Biology, University of Massachusetts, Amherst, Amherst, Massachusetts 01003, USA
    Curr Biol 16:536-41. 2006
    ..The presence of microtubules at kinetochores provides a mechanism to reconcile the time required for spindle assembly in vivo with that observed in computer simulations of search and capture...
  18. ncbi request reprint Myosin-II-dependent localization and dynamics of F-actin during cytokinesis
    Kausalya Murthy
    Department of Biology and Program in Molecular and Cellular Biology, University of Massachusetts, Amherst, 01003, USA
    Curr Biol 15:724-31. 2005
    ..The mechanism(s) that contribute to the spatially and temporally regulated assembly and disassembly of the CR at the cell equator are poorly understood...
  19. pmc Prophase microtubule arrays undergo flux-like behavior in mammalian cells
    Nick P Ferenz
    Department of Biology and Program in Molecular and Cellular Biology, University of Massachusetts, Amherst, MA 01003, USA
    Mol Biol Cell 18:3993-4002. 2007
    ..We propose that the marked reduction in rate and directionality of microtubule motion from prophase to metaphase results from changes in microtubule organization during spindle formation...
  20. pmc Poleward transport of TPX2 in the mammalian mitotic spindle requires dynein, Eg5, and microtubule flux
    Nan Ma
    University of Massachusetts, Amherst, MA 01003, USA
    Mol Biol Cell 21:979-88. 2010
    ..Our data support a model in which poleward transport of TPX2 down-regulates its microtubule nucleating activity near kinetochores and links microtubules generated at kinetochores to dynein for incorporation into the spindle...
  21. pmc Dynein antagonizes eg5 by crosslinking and sliding antiparallel microtubules
    Nick P Ferenz
    Department of Biology, University of Massachusetts Amherst, Amherst, MA 01003, USA
    Curr Biol 19:1833-8. 2009
    ..Our results suggest that dynein functionally coordinates with Eg5 by crosslinking and sliding antiparallel MTs, a novel role for dynein within the framework of spindle assembly...
  22. pmc Dual role for microtubules in regulating cortical contractility during cytokinesis
    Kausalya Murthy
    Department of Biology and Program in Molecular and Cellular Biology, University of Massachusetts at Amherst, Amherst, MA 01003, USA
    J Cell Sci 121:2350-9. 2008
    ..Wave-like contractile behavior in anaphase cells was Rho-dependent. We conclude that dynamic astral microtubules function to suppress Rho activation in the non-equatorial cortex, limiting the contractile activity of the polar cortex...
  23. pmc Living microlens arrays
    Jessica A Zimberlin
    Department of Polymer Science and Engineering, University of Massachusetts, Amherst, Massachusetts 01003, USA
    Cell Motil Cytoskeleton 65:762-7. 2008
    ..Together, these findings demonstrate the importance of studying the mechanics of cell sheets and also introduce a new design paradigm for advanced materials, offering great promise for a range of applications...
  24. pmc Imaging protein dynamics in live mitotic cells
    Nick P Ferenz
    Department of Biology, University of Massachusetts, Amherst, MA 01003, USA
    Methods 51:193-6. 2010
    ..We provide examples of photoactivable proteins in mammalian and yeast cells to illustrate the power of this approach to examine the dynamics of spindle formation and function in diverse cells...
  25. ncbi request reprint Cytokinesis: Rho marks the spot
    Patricia Wadsworth
    Biology Department, University of Massachusetts, Amherst 01003 5810, USA
    Curr Biol 15:R871-4. 2005
    ..Recent studies have provided new insights into the mechanism by which the site of contractile ring assembly is specified...
  26. ncbi request reprint E pluribus unum: towards a universal mechanism for spindle assembly
    Patricia Wadsworth
    Morrill Science Center, Department of Biology, University of Massachusetts, Amherst, MA 01003, USA
    Trends Cell Biol 14:413-9. 2004
    ....
  27. pmc Kinetochore dynein is required for chromosome motion and congression independent of the spindle checkpoint
    Zhenye Yang
    Lab of Cell Growth and Division, Division of Molecular Medicine, Wadsworth Center, New York State Department of Health, Albany, New York 12201 0509, USA
    Curr Biol 17:973-80. 2007
    ..Thus, in addition to its role in silencing the SAC, KD is important for forming and stabilizing K fibers and in powering chromosome motion...
  28. ncbi request reprint Noscapine alters microtubule dynamics in living cells and inhibits the progression of melanoma
    Jaren W Landen
    Emory University School of Medicine, Atlanta, Georgia 30322, USA
    Cancer Res 62:4109-14. 2002
    ..Consequently, noscapine could be a valuable chemotherapeutic agent, alone or in combination, for the treatment of advanced melanoma...

Research Grants10

  1. Purchase of Leica TCS SP2 Confocal Microscope
    Patricia Wadsworth; Fiscal Year: 2002
    ....
  2. Mechanisms of Microtubule Dynamic Turnover
    Patricia Wadsworth; Fiscal Year: 2009
    ..Learning how normal cells construct a mitotic spindle is an important first step towards understanding defective cell division in cancer. ..
  3. MECHANISM AND REGULATION OF MICROTUBULE TURNOVER
    Patricia Wadsworth; Fiscal Year: 2003
    ..Understanding these processes in normal cells is an important first step towards analysis of cancer cells, where such processes are aberrant. ..