Michael J Wacker
Affiliation: University of Missouri-Kansas City
- Cardiac thromboxane A2 receptor activation does not directly induce cardiomyocyte hypertrophy but does cause cell death that is prevented with gentamicin and 2-APBChad D Touchberry
Muscle Biology Group, School of Medicine, University of Missouri Kansas City, 2464 Charlotte Street, Kansas City, MO 64108, USA
BMC Pharmacol Toxicol 15:73. 2014..Since TXA2 is an inflammatory mediator and induces direct calcium changes in cardiomyocytes, we hypothesized that TXA2 released during ischemia or inflammation could also cause cardiac remodeling...
- Technique for quantitative RT-PCR analysis directly from single muscle fibersMichael J Wacker
Department of Basic Medical Science, University of Missouri Kansas City School of Medicine, Kansas City, MO 64108, USA
J Appl Physiol (1985) 105:308-15. 2008..9-fold change in expression after resistance exercise, which is consistent with what has been previously observed. These results demonstrate a successful method for gene expression analysis directly from single muscle fibers...
- Sex difference in the association of the angiotensin converting enzyme I/D polymorphism and body mass indexMichael J Wacker
Department of Basic Medical Science, School of Medicine, University of Missouri Kansas City, Kansas City, MO 64108, USA
Med Sci Monit 14:CR353-7. 2008..ACE and the components of the renin-angiotensin system are expressed in adipose tissue and therefore the I/D polymorphism within ACE may be associated with obesity...
- Inhibition of thromboxane A2-induced arrhythmias and intracellular calcium changes in cardiac myocytes by blockade of the inositol trisphosphate pathwayM J Wacker
Muscle Biology Research Group, School of Medicine, University of Missouri Kansas City, Kansas City, Missouri 64108, USA
J Pharmacol Exp Ther 331:917-24. 2009..In summary, this study provides novel evidence for direct TXA(2)-induced cardiac arrhythmias and provides a rationale for IP(3) as a potential target for the treatment of TXA(2)-mediated arrhythmias...
- Store-operated calcium entry is present in HL-1 cardiomyocytes and contributes to resting calciumChad D Touchberry
School of Medicine, Muscle Biology Research Group, University of Missouri Kansas City, Kansas City, MO 64108, USA
Biochem Biophys Res Commun 416:45-50. 2011..Currently, there is little knowledge about SOCE in cardiomyocytes, and the present results suggest that HL-1 cells will be of great utility in investigating the role of SOCE in the heart...
- FGF23 is a novel regulator of intracellular calcium and cardiac contractility in addition to cardiac hypertrophyChad D Touchberry
Muscle Biology Group, School of Medicine, University of Missouri Kansas City, Kansas City, MO 64108, USA
Am J Physiol Endocrinol Metab 304:E863-73. 2013..In conclusion, FGF23 is a novel bone/heart endocrine factor and may be an important mediator of cardiac Ca(2+) regulation and contractile function during chronic kidney disease...
- Phosphatidylinositol 3,5-bisphosphate increases intracellular free Ca2+ in arterial smooth muscle cells and elicits vasocontractionNeerupma Silswal
Basic Medical Science Dept, Muscle Biology Research Group, School of Medicine, Univ of Missouri, Kansas City, MO 64108, USA
Am J Physiol Heart Circ Physiol 300:H2016-26. 2011..Thus, full and sustained aortic contractions to PI(3,5)P(2) required the release of SR Ca(2+), probably via the activation of RyR, and also extracellular Ca(2+) entry via voltage-dependent Ca(2+) channels...
- FGF23 directly impairs endothelium-dependent vasorelaxation by increasing superoxide levels and reducing nitric oxide bioavailabilityNeerupma Silswal
Muscle Biology Group, School of Medicine, University of Missouri Kansas City, Kansas City, Missouri and
Am J Physiol Endocrinol Metab 307:E426-36. 2014..Together, these data provide evidence that high levels of FGF23 contribute to cardiovascular dysfunction. ..
- PPARα-Independent Arterial Smooth Muscle Relaxant Effects of PPARα AgonistsNeerupma Silswal
Muscle Biology Research Group MUBIG, Basic Medical Science Department, School of Medicine, University of Missouri Kansas City, Kansas City, MO 64108, USA
PPAR Res 2012:302495. 2012..Responses to PPARα agonists in the aorta involved K(ATP) channels and sGC, whereas in the MCA the PKC and sGC pathways also appeared to contribute to the response...
- FGF23 production by osteocytesLynda F Bonewald
School of Dentistry, University of Missouri, 650 East 25th Street, Kansas City, MO, 64108 2784, USA
Pediatr Nephrol 28:563-8. 2013..In this review we discuss FGF23 expression in osteocytes and the potential means to regulate expression and function of this protein at the osteocyte level...
- Phosphatidylinositol 3,5-bisphosphate (PI(3,5)P2) potentiates cardiac contractility via activation of the ryanodine receptorChad D Touchberry
Schools of Medicine, University of Missouri, Kansas City, Missouri 64108, USA
J Biol Chem 285:40312-21. 2010..Thus, we provide inaugural evidence that PI(3,5)P2 is a powerful activator of sarcoplasmic reticulum Ca(2+) release and thereby modulates cardiac contractility...
- Characterization of thromboxane A₂ receptor and TRPV1 mRNA expression in cultured sensory neuronsMichael J Wacker
Department of Basic Medical Science, University of Missouri Kansas City School of Medicine, Kansas City, MO 64108, United States
Neurosci Lett 515:12-7. 2012..Our data also indicate that TxA(2) is likely to stimulate both nociceptive and non-nociceptive neurons thereby broadening the types of neurons and reflexes that it may excite...
- Age-related changes in relative expression of real-time PCR housekeeping genes in human skeletal muscleChad D Touchberry
Applied Physiology Laboratory, University of Kansas, Department of Health Sport and Exercise Sciences, Lawrence, KS, USA
J Biomol Tech 17:157-62. 2006..51). The results indicate that special consideration needs to be made when selecting housekeeping genes for comparisons in real-time reverse-transcriptase polymerase chain reaction, depending upon the age of the populations of interest...
- Analysis of one-step and two-step real-time RT-PCR using SuperScript IIIMichael J Wacker
Applied Physiology Laboratory, 5059 Haworth Hall, 1200 Sunnyside Ave, University of Kansas, Lawrence, KS 66045, USA
J Biomol Tech 16:266-71. 2005..However, using the one-step method with gene-specific priming may be more sensitive for quantification of certain genes such as PolR2A...
- Detection of thromboxane A(2) receptor mRNA in rabbit nodose ganglion neuronsMichael J Wacker
Department of Molecular Biosciences, University of Kansas, 1200 Sunnyside Avenue, Lawrence, KS 66045, USA
Neurosci Lett 386:121-6. 2005..Therefore, we conclude that the presence of TP mRNA in a subset of cultured nodose ganglion neurons allows for the possibility that TXA(2) may directly stimulate or modulate vagal afferent neurons...
- Contributions of the ubiquitin-proteasome pathway and apoptosis to human skeletal muscle wasting with ageSamantha A Whitman
Applied Physiology Laboratory, University of Kansas, 1301 Sunnyside Ave, Robinson Center Room 101DJ, Lawrence, KS 66045, USA
Pflugers Arch 450:437-46. 2005..Muscle strength was also significantly lower in old compared to young subjects (p < 0.05). In conclusion, this study indicates a preferential role for apoptosis contributing to decreases in muscle function with age...