Stefanie Vogel

Summary

Affiliation: University of Maryland
Country: USA

Publications

  1. pmc Modulation of hepatic PPAR expression during Ft LVS LPS-induced protection from Francisella tularensis LVS infection
    Saroj K Mohapatra
    Virginia Bioinformatics Institute, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA
    BMC Infect Dis 10:10. 2010
  2. ncbi request reprint Mutations in TLR4 signaling that lead to increased susceptibility to infection in humans: an overview
    Stefanie N Vogel
    Department of Microbiology and Immunology, University of Maryland, Baltimore, Baltimore, MD 21201, USA
    J Endotoxin Res 11:333-9. 2005
  3. ncbi request reprint TLRs: differential adapter utilization by toll-like receptors mediates TLR-specific patterns of gene expression
    Stefanie N Vogel
    Departments of Microbiology and Immunology and Medicine, University of Maryland, Baltimore, MD, USA
    Mol Interv 3:466-77. 2003
  4. ncbi request reprint Toll-like receptor 4 signalling: new perspectives on a complex signal-transduction problem
    S N Vogel
    Department of Microbiology and Immunology, and Department of Medicine, University of Maryland, Baltimore, 655 W Baltimore Street 13 009, Baltimore, MD 21201, USA
    Biochem Soc Trans 31:664-8. 2003
  5. ncbi request reprint TLR4, but not TLR2, mediates IFN-beta-induced STAT1alpha/beta-dependent gene expression in macrophages
    Vladimir Toshchakov
    Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA
    Nat Immunol 3:392-8. 2002
  6. ncbi request reprint Dysregulation of LPS-induced Toll-like receptor 4-MyD88 complex formation and IL-1 receptor-associated kinase 1 activation in endotoxin-tolerant cells
    Andrei E Medvedev
    Department of Microbiology and Immunology, University of Maryland, Baltimore 21201, USA
    J Immunol 169:5209-16. 2002
  7. ncbi request reprint Differential involvement of BB loops of toll-IL-1 resistance (TIR) domain-containing adapter proteins in TLR4- versus TLR2-mediated signal transduction
    Vladimir U Toshchakov
    Department of Microbiology and Immunology, University of Maryland, Baltimore, MD 21201, USA
    J Immunol 175:494-500. 2005
  8. ncbi request reprint Overexpression of CD14, TLR4, and MD-2 in HEK 293T cells does not prevent induction of in vitro endotoxin tolerance
    Andrei E Medvedev
    Department of Microbiology and Immunology, University of Maryland, Baltimore 21201, USA
    J Endotoxin Res 9:60-4. 2003
  9. ncbi request reprint TLR2 and TLR4 agonists stimulate unique repertoires of host resistance genes in murine macrophages: interferon-beta-dependent signaling in TLR4-mediated responses
    Vladimir Toshchakov
    Department of Microbiology and Immunology, University of Maryland, 655 W Baltimore Street, Baltimore, MD 21201, USA
    J Endotoxin Res 9:169-75. 2003
  10. ncbi request reprint Cutting edge: expression of IL-1 receptor-associated kinase-4 (IRAK-4) proteins with mutations identified in a patient with recurrent bacterial infections alters normal IRAK-4 interaction with components of the IL-1 receptor complex
    Andrei E Medvedev
    Department of Microbiology and Immunology, University of Maryland, Baltimore, 21201, USA
    J Immunol 174:6587-91. 2005

Research Grants

Detail Information

Publications77

  1. pmc Modulation of hepatic PPAR expression during Ft LVS LPS-induced protection from Francisella tularensis LVS infection
    Saroj K Mohapatra
    Virginia Bioinformatics Institute, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA
    BMC Infect Dis 10:10. 2010
    ..It has been shown previously that administration of Francisella tularensis (Ft) Live Vaccine Strain (LVS) lipopolysaccharide (LPS) protects mice against subsequent challenge with Ft LVS and blunts the pro-inflammatory cytokine response...
  2. ncbi request reprint Mutations in TLR4 signaling that lead to increased susceptibility to infection in humans: an overview
    Stefanie N Vogel
    Department of Microbiology and Immunology, University of Maryland, Baltimore, Baltimore, MD 21201, USA
    J Endotoxin Res 11:333-9. 2005
    ..To date, mutations in the extracellular domain of TLR4 itself, IRAK-4, NEMO (IKK gamma), and I kappa B alpha have been identified and profoundly affect the host response to infection...
  3. ncbi request reprint TLRs: differential adapter utilization by toll-like receptors mediates TLR-specific patterns of gene expression
    Stefanie N Vogel
    Departments of Microbiology and Immunology and Medicine, University of Maryland, Baltimore, MD, USA
    Mol Interv 3:466-77. 2003
    ..Given the rapidity with which such interactions have been described, we have attempted to summarize our current understanding of the adapters that are so essential for TLR signaling and provide a working model for future studies...
  4. ncbi request reprint Toll-like receptor 4 signalling: new perspectives on a complex signal-transduction problem
    S N Vogel
    Department of Microbiology and Immunology, and Department of Medicine, University of Maryland, Baltimore, 655 W Baltimore Street 13 009, Baltimore, MD 21201, USA
    Biochem Soc Trans 31:664-8. 2003
    ..Collectively, our findings provide the first mechanistic basis for differential patterns of gene expression activated by TLR4 and TLR2 agonists...
  5. ncbi request reprint TLR4, but not TLR2, mediates IFN-beta-induced STAT1alpha/beta-dependent gene expression in macrophages
    Vladimir Toshchakov
    Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA
    Nat Immunol 3:392-8. 2002
    ..Together, these findings provide the first mechanistic basis for differential patterns of gene expression activated by TLR4 and TLR2 agonists...
  6. ncbi request reprint Dysregulation of LPS-induced Toll-like receptor 4-MyD88 complex formation and IL-1 receptor-associated kinase 1 activation in endotoxin-tolerant cells
    Andrei E Medvedev
    Department of Microbiology and Immunology, University of Maryland, Baltimore 21201, USA
    J Immunol 169:5209-16. 2002
    ..Thus, decreased TLR4-MyD88 complex formation with subsequent impairment of IRAK-1 activity may underlie the LPS-tolerant phenotype...
  7. ncbi request reprint Differential involvement of BB loops of toll-IL-1 resistance (TIR) domain-containing adapter proteins in TLR4- versus TLR2-mediated signal transduction
    Vladimir U Toshchakov
    Department of Microbiology and Immunology, University of Maryland, Baltimore, MD 21201, USA
    J Immunol 175:494-500. 2005
    ..Our data suggest that the interactions required for a fully functional TLR4 signaling "platform" are disrupted by these BPs, and that the adapter BB loops may serve distinct roles in TLR4 and TLR2 signalosome assembly...
  8. ncbi request reprint Overexpression of CD14, TLR4, and MD-2 in HEK 293T cells does not prevent induction of in vitro endotoxin tolerance
    Andrei E Medvedev
    Department of Microbiology and Immunology, University of Maryland, Baltimore 21201, USA
    J Endotoxin Res 9:60-4. 2003
    ..Thus, altered expression and/or function of intracellular mediators downstream of the TLR4/MD-2 complex play an important role in mediating LPS tolerance...
  9. ncbi request reprint TLR2 and TLR4 agonists stimulate unique repertoires of host resistance genes in murine macrophages: interferon-beta-dependent signaling in TLR4-mediated responses
    Vladimir Toshchakov
    Department of Microbiology and Immunology, University of Maryland, 655 W Baltimore Street, Baltimore, MD 21201, USA
    J Endotoxin Res 9:169-75. 2003
    ....
  10. ncbi request reprint Cutting edge: expression of IL-1 receptor-associated kinase-4 (IRAK-4) proteins with mutations identified in a patient with recurrent bacterial infections alters normal IRAK-4 interaction with components of the IL-1 receptor complex
    Andrei E Medvedev
    Department of Microbiology and Immunology, University of Maryland, Baltimore, 21201, USA
    J Immunol 174:6587-91. 2005
    ..Therefore, mimetics of these truncated IRAK-4 proteins may represent a novel approach to mitigating hyperinflammatory states...
  11. ncbi request reprint Toll receptors, CD14, and macrophage activation and deactivation by LPS
    Marina A Dobrovolskaia
    Department of Microbiology and Immunology, Uniformed Services University of Health Sciences, 4301 Jones Bridge Road, Bethesda, MD 20814, USA
    Microbes Infect 4:903-14. 2002
    ..The most recent advances in the understanding of the function of the LPS receptor complex and its role in the development of the septic shock syndrome and endotoxin tolerance will be discussed...
  12. ncbi request reprint Bordetella pertussis adenylate cyclase toxin (ACT) induces cyclooxygenase-2 (COX-2) in murine macrophages and is facilitated by ACT interaction with CD11b/CD18 (Mac-1)
    Darren J Perkins
    Department of Microbiology and Immunology, University of Maryland, Baltimore UMB, School of Medicine, 660 W Baltimore Street, Suite 324, Baltimore, MD 21201, USA
    Mol Microbiol 66:1003-15. 2007
    ..pertussis infection by augmenting COX-2 expression and provides evidence against the concept that ACT functions exclusively via its inhibitory effects on phagocytic leucocytes...
  13. ncbi request reprint Tolerance to microbial TLR ligands: molecular mechanisms and relevance to disease
    Andrei E Medvedev
    Department of Microbiology and Immunology, University of Maryland, Baltimore UMB, Baltimore, Maryland 21201, USA
    J Endotoxin Res 12:133-50. 2006
    ..In this review, we present current theories of the molecular mechanisms that underlie induction and maintenance of 'microbial tolerance', and discuss the possible relevance of tolerance to several infectious and non-infectious diseases...
  14. ncbi request reprint Cell-penetrating TIR BB loop decoy peptides a novel class of TLR signaling inhibitors and a tool to study topology of TIR-TIR interactions
    Vladimir Y Toshchakov
    University of Maryland, Department of Microbiology and Immunology, School of Medicine, MD 21201 1559, Baltimore, USA
    Expert Opin Biol Ther 7:1035-50. 2007
    ..This review discusses cell-penetrating decoy peptides as a new tool to further understanding of the molecular interactions required for TLR signaling and evaluates the potential of this approach for the creation of therapeutic agents...
  15. pmc Role of TLR4 tyrosine phosphorylation in signal transduction and endotoxin tolerance
    Andrei E Medvedev
    Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
    J Biol Chem 282:16042-53. 2007
    ..Thus, our data demonstrate that TLR4 tyrosine phosphorylation is important for signaling and is impaired in endotoxin-tolerant cells, and suggest involvement of Lyn kinase in these processes...
  16. ncbi request reprint Mastoparan, a G protein agonist peptide, differentially modulates TLR4- and TLR2-mediated signaling in human endothelial cells and murine macrophages
    Arnd Lentschat
    Department of Microbiology and Immunology, University of Maryland, Baltimore, MD 21201, USA
    J Immunol 174:4252-61. 2005
    ....
  17. ncbi request reprint Analysis of TLR4 polymorphic variants: new insights into TLR4/MD-2/CD14 stoichiometry, structure, and signaling
    Prasad Rallabhandi
    Department of Microbiology and Immunology, University of Maryland, 660 West Redwood Street, Baltimore, MD 21201, USA
    J Immunol 177:322-32. 2006
    ..A structural model that accounts for the diminished responsiveness of mutant TLR4 polymorphisms to structurally unrelated TLR4 agonists is proposed...
  18. ncbi request reprint Tobacco smoking inhibits expression of proinflammatory cytokines and activation of IL-1R-associated kinase, p38, and NF-kappaB in alveolar macrophages stimulated with TLR2 and TLR4 agonists
    Haiyan Chen
    Department of Microbiology and Immunology, University of Maryland, Baltimore, Baltimore, MD 21201, USA
    J Immunol 179:6097-106. 2007
    ....
  19. ncbi request reprint Induction of in vitro reprogramming by Toll-like receptor (TLR)2 and TLR4 agonists in murine macrophages: effects of TLR "homotolerance" versus "heterotolerance" on NF-kappa B signaling pathway components
    Marina A Dobrovolskaia
    Department of Microbiology and Immunology, University of Maryland, Baltimore, MD 21201, USA
    J Immunol 170:508-19. 2003
    ..Collectively, these data demonstrate that induction of homotolerance affects a broader spectrum of signaling components than in heterotolerance, with selective modulation of specific elements within the NF-kappaB signaling pathway...
  20. pmc Analysis of proteinase-activated receptor 2 and TLR4 signal transduction: a novel paradigm for receptor cooperativity
    Prasad Rallabhandi
    Department of Microbiology and Immunology, University of Maryland, Baltimore, Maryland 21201, USA
    J Biol Chem 283:24314-25. 2008
    ..Our data suggest a unique interaction between two distinct innate immune response receptors and support a novel paradigm of receptor cooperativity in inflammatory responses...
  21. ncbi request reprint The role of the interferon regulatory factors, IRF-1 and IRF-2, in LPS-induced cyclooxygenase-2 (COX-2) expression in vivo and in vitro
    Shuling Zhang
    Department of Microbiology and Immunology, University of Maryland, Baltimore, Maryland 21201, USA
    J Endotoxin Res 8:379-88. 2002
    ..Collectively, these data provide evidence for an important role for IRF-1 in vivo and in vitro and for IRF-2 in vitro in the regulation of COX-2 expression by LPS...
  22. ncbi request reprint Regulation of lipopolysaccharide sensitivity by IFN regulatory factor-2
    Natalia Cuesta
    Department of Microbiology and Immunology, University of Maryland, Baltimore, MD 21201, USA
    J Immunol 170:5739-47. 2003
    ..Collectively, these findings reveal a critical role for IRF-2 in endotoxicity, and point to a previously unappreciated role for IRF-2 in the regulation of apoptosis...
  23. ncbi request reprint Gliadin stimulation of murine macrophage inflammatory gene expression and intestinal permeability are MyD88-dependent: role of the innate immune response in Celiac disease
    Karen E Thomas
    Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, 21201, USA
    J Immunol 176:2512-21. 2006
    ....
  24. pmc Febrile-range temperature modifies cytokine gene expression in LPS-stimulated macrophages by differentially modifying NF-{kappa}B recruitment to cytokine gene promoters
    Zachary A Cooper
    Univ of Maryland School of Medicine, Health Science Facility II, Rm S311, 20 Penn St, Baltimore, MD 21201, USA
    Am J Physiol Cell Physiol 298:C171-81. 2010
    ..These data suggest that FRT exerts its effects on cytokine gene expression in a gene-specific manner through distal effects on promoter activation rather than proximal receptor activation and signal transduction...
  25. ncbi request reprint Cutting Edge: Differential inhibition of TLR signaling pathways by cell-permeable peptides representing BB loops of TLRs
    Vladimir Y Toshchakov
    Department of Microbiology and Immunology, University of Maryland Baltimore, 660 West Redwood Street, Baltimore, MD 21201, USA
    J Immunol 178:2655-60. 2007
    ..Our results suggest that the receptor BBPs selectively affect different TLR signaling pathways, and that the BB loops of TLR1/6 and TLR2 play distinct roles in formation of receptor heterodimers and recruitment of adaptor proteins...
  26. pmc TLR4/MyD88/PI3K interactions regulate TLR4 signaling
    Michelle H W Laird
    University of Maryland, Baltimore, 660 W Redwood Street, Room 324, Baltimore, MD 21201, USA
    J Leukoc Biol 85:966-77. 2009
    ..We propose a hypothetical model in which sustained PI3K activity at the membrane limits the availability of the PI3K substrate, thereby negatively regulating signaling...
  27. pmc Phagosomal retention of Francisella tularensis results in TIRAP/Mal-independent TLR2 signaling
    Leah E Cole
    Department of Microbiology and Immunology, University of Maryland, Baltimore, Baltimore, MD 21201, USA
    J Leukoc Biol 87:275-81. 2010
    ..Taken together, these data suggest that prolonging or enhancing the interaction between TLR2 and its agonist overcomes the "bridging" function ascribed previously to TIRAP...
  28. pmc Differential activation of human TLR4 by Escherichia coli and Shigella flexneri 2a lipopolysaccharide: combined effects of lipid A acylation state and TLR4 polymorphisms on signaling
    Prasad Rallabhandi
    Department of Microbiology and Immunology, Center for Vaccine Development, University of Maryland, Baltimore 21201, USA
    J Immunol 180:1139-47. 2008
    ..Thus, the combination of acylation state and host genetics may significantly impact vaccine immunogenicity and/or efficacy, whether LPS is an integral component of a whole organism vaccine or included as an adjuvant...
  29. ncbi request reprint Immunologic consequences of Francisella tularensis live vaccine strain infection: role of the innate immune response in infection and immunity
    Leah E Cole
    Department of Microbiology and Immunology, University of Maryland, Baltimore, School of Medicine, Baltimore, MD 21201, USA
    J Immunol 176:6888-99. 2006
    ....
  30. pmc Distinct mutations in IRAK-4 confer hyporesponsiveness to lipopolysaccharide and interleukin-1 in a patient with recurrent bacterial infections
    Andrei E Medvedev
    Department of Microbiology and Immunology, University of Maryland, Baltimore, Baltimore, MD 20101, USA
    J Exp Med 198:521-31. 2003
    ..Thus, IRAK-4 is pivotal in the development of a normal inflammatory response initiated by bacterial or nonbacterial insults...
  31. ncbi request reprint Role of phosphatidylinositol-3 kinase in transcriptional regulation of TLR-induced IL-12 and IL-10 by Fc gamma receptor ligation in murine macrophages
    Swamy Kumar Polumuri
    Department of Microbiology and Immunology, School of Medicine, University of Maryland, 660 West Redwood Street, Baltimore, MD 21201, USA
    J Immunol 179:236-46. 2007
    ..Thus, FcgammaR ligation modulates LPS-induced IL-12 via multiple PI3K-sensitive pathways that affect production, accumulation, and binding of key DNA-binding proteins required for IL-12 induction...
  32. pmc Vibrio cholerae flagellins induce Toll-like receptor 5-mediated interleukin-8 production through mitogen-activated protein kinase and NF-kappaB activation
    Lisa M Harrison
    Department of Microbiology and Immunology, University of Maryland Baltimore School of Medicine, Baltimore, Maryland 21201, USA
    Infect Immun 76:5524-34. 2008
    ..Collectively, these data suggest that V. cholerae flagellins are present in culture supernatants and can induce TLR5- and MAPK-dependent IL-8 secretion in host cells...
  33. ncbi request reprint Transcriptional regulation of lipopolysaccharide (LPS)-induced Toll-like receptor (TLR) expression in murine macrophages: role of interferon regulatory factors 1 (IRF-1) and 2 (IRF-2)
    Quan M Nhu
    Department of Microbiology and Immunology, University of Maryland Baltimore UMB, School of Medicine, Baltimore, MD 21201, USA
    J Endotoxin Res 12:285-95. 2006
    ....
  34. ncbi request reprint The proteasome: a central regulator of inflammation and macrophage function
    Nilofer Qureshi
    Department of Basic Medical Science, School of Medicine, Shock Trauma Research Center, University of Maryland Baltimore, Baltimore, MD, USA
    Immunol Res 31:243-60. 2005
    ..Taken collectively, the results suggest a potentially important role of the proteasome in inflammation and other macrophage functions...
  35. ncbi request reprint A role for Stat1 in the regulation of lipopolysaccharide-induced interleukin-1beta expression
    Vishwas D Joshi
    Department of Medicine, Division of Geographic Medicine, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    J Interferon Cytokine Res 26:739-47. 2006
    ....
  36. pmc Bacillus anthracis spores and lethal toxin induce IL-1beta via functionally distinct signaling pathways
    Tae Jin Kang
    Center for Vaccine Development, Department of Medicine, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    Eur J Immunol 38:1574-84. 2008
    ..Thus different components of the same bacterium each induce IL-1beta by distinct signaling pathways. Whereas the spore-induced IL-1beta limits BA infection, LT-induced IL-1beta enables BA to escape host defenses...
  37. ncbi request reprint Contribution of interferon-beta to the murine macrophage response to the toll-like receptor 4 agonist, lipopolysaccharide
    Karen E Thomas
    Department of Microbiology and Immunology, University of Maryland, Baltimore, Maryland 21201, USA
    J Biol Chem 281:31119-30. 2006
    ..g. TLR4(-/-) or TRIF(-/-) mice). Collectively, these findings reveal unanticipated regulatory roles for IFN-beta in response to LPS in vitro and in vivo...
  38. pmc TLR4 signaling is coupled to SRC family kinase activation, tyrosine phosphorylation of zonula adherens proteins, and opening of the paracellular pathway in human lung microvascular endothelia
    Ping Gong
    Department of Pathology, University of Maryland, Baltimore, Maryland 21201, USA
    J Biol Chem 283:13437-49. 2008
    ..These data indicate that LPS recognition by TLR4 activates the SFKs, c-SRC, FYN, and YES, which, in turn, contribute to tyrosine phosphorylation of zonula adherens proteins to open the endothelial paracellular pathway...
  39. ncbi request reprint Up-regulation of human monocyte CD163 upon activation of cell-surface Toll-like receptors
    Lehn K Weaver
    Department of Microbiology and Immunology, Dartmouth Medical School, HB7700, Lebanon, NH 03756, USA
    J Leukoc Biol 81:663-71. 2007
    ..Thus, surface CD163 expression on mononuclear phagocytes is a carefully regulated component of the innate immune response to infection...
  40. pmc Type I IL-4Rs selectively activate IRS-2 to induce target gene expression in macrophages
    Nicola M Heller
    Center for Vascular and Inflammatory Diseases, Marlene and Stewart Greenebaum Cancer Center, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    Sci Signal 1:ra17. 2008
    ..Thus, IL-4 activates signaling pathways through type I IL-4Rs qualitatively differently from IL-13, which cooperate to induce optimal gene expression...
  41. pmc The anti-tumor agent, 5,6-dimethylxanthenone-4-acetic acid (DMXAA), induces IFN-beta-mediated antiviral activity in vitro and in vivo
    Kari Ann Shirey
    Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
    J Leukoc Biol 89:351-7. 2011
    ..Collectively, this study provides data to support the use of DMXAA as a novel antiviral agent...
  42. pmc Role of TLR signaling in Francisella tularensis-LPS-induced, antibody-mediated protection against Francisella tularensis challenge
    Leah E Cole
    Department of Microbiology and Immunology, University of Maryland, Baltimore, MD 21201, USA
    J Leukoc Biol 90:787-97. 2011
    ..e., production of Ft-LPS-specific antibody, as well as TLR-mediated macrophage activation, to fully control Francisella infection...
  43. pmc Macrophage proinflammatory response to Francisella tularensis live vaccine strain requires coordination of multiple signaling pathways
    Leah E Cole
    Department of Microbiology and Immunology, School of Medicine, University of Maryland, Baltimore, MD 21201, USA
    J Immunol 180:6885-91. 2008
    ....
  44. pmc Mice deficient in the CXCR2 ligand, CXCL1 (KC/GRO-alpha), exhibit increased susceptibility to dextran sodium sulfate (DSS)-induced colitis
    Terez Shea-Donohue
    Department of Medicine, University of Maryland, School of Medicine, Baltimore, MD 21201, USA
    Innate Immun 14:117-24. 2008
    ..This finding implies that neutrophil infiltration induced by CXCL1/KC is an essential component of the intestinal response to inflammatory stimuli as well as the ability of the intestine to restore mucosal barrier integrity...
  45. pmc Francisella tularensis live vaccine strain induces macrophage alternative activation as a survival mechanism
    Kari Ann Shirey
    Department of Microbiology and Immunology, University of Maryland, School of Medicine, Baltimore, MD 21201, USA
    J Immunol 181:4159-67. 2008
    ..Thus, redirection of macrophage differentiation by Ft LVS from a classical to an alternative activation state enables the organism to survive at the expense of the host...
  46. ncbi request reprint Association of TLR4 polymorphisms with symptomatic respiratory syncytial virus infection in high-risk infants and young children
    Agnes A Awomoyi
    Department of Microbiology and Immunology, University of Maryland Baltimore, 660 W Redwood Street, Baltimore, MD 21201, USA
    J Immunol 179:3171-7. 2007
    ....
  47. pmc Antigen-specific B-1a antibodies induced by Francisella tularensis LPS provide long-term protection against F. tularensis LVS challenge
    Leah E Cole
    Department of Microbiology and Immunology, University of Maryland, Baltimore, MD 21201, USA
    Proc Natl Acad Sci U S A 106:4343-8. 2009
    ..These data support the possibility of creating effective, minimally invasive vaccines that can provide effective protection against pathogen invasion...
  48. pmc The chemotherapeutic agent DMXAA potently and specifically activates the TBK1-IRF-3 signaling axis
    Zachary J Roberts
    Department of Microbiology and Immunology, University of Maryland Baltimore, Baltimore, MD 21201, USA
    J Exp Med 204:1559-69. 2007
    ..These findings detail a novel pathway for TBK1-mediated IRF-3 activation and provide new insights into the mechanism of this new class of chemotherapeutic drugs...
  49. pmc Toll-like receptor 2-mediated signaling requirements for Francisella tularensis live vaccine strain infection of murine macrophages
    Leah E Cole
    Department of Microbiology and Immunology, University of Maryland, Baltimore, 660 West Redwood Street, Room 324, Baltimore, MD 21201, USA
    Infect Immun 75:4127-37. 2007
    ..Collectively, these data indicate that the primary macrophage response to F. tularensis LVS is overwhelmingly TLR2 dependent, requires de novo bacterial protein synthesis, and is independent of intracellular F. tularensis replication...
  50. pmc Annexin A2 tetramer activates human and murine macrophages through TLR4
    Jennifer F A Swisher
    Laboratory of Molecular and Developmental Immunology, Division of Monoclonal Antibodies, Office of Biotechnology Products, Center for Drug Evaluation and Research, Food and Drug Administration, Bethesda, MD, USA
    Blood 115:549-58. 2010
    ....
  51. ncbi request reprint IFN regulatory factor-2 regulates macrophage apoptosis through a STAT1/3- and caspase-1-dependent mechanism
    Natalia Cuesta
    Department of Microbiology and Immunology, University of Maryland School of Medicine, 600 West Baltimore Street, Baltimore, MD 21201, USA
    J Immunol 178:3602-11. 2007
    ..Collectively, these data support the hypothesis that IRF-2 acts as a transcriptional repressor of Casp1, and that the absence of IRF-2 renders macrophages more sensitive to apoptotic stimuli in a caspase-1-dependent process...
  52. pmc Characterization of rationally attenuated Francisella tularensis vaccine strains that harbor deletions in the guaA and guaB genes
    Araceli E Santiago
    Center for Vaccine Development, University of Maryland, Baltimore, 21201, United States
    Vaccine 27:2426-36. 2009
    ..These findings suggest the specific deletion of these target genes could generate a safe and efficacious live attenuated vaccine...
  53. ncbi request reprint Selective roles for Toll-like receptor (TLR)2 and TLR4 in the regulation of neutrophil activation and life span
    Ian Sabroe
    Academic Unit of Respiratory Medicine, Section of Functional Genomics, Division of Genomic Medicine, University of Sheffield, Sheffield, United Kingdom
    J Immunol 170:5268-75. 2003
    ..TLR4 signaling presents itself as a pharmacological target that may allow therapeutic modulation of neutrophil survival by direct and indirect mechanisms at sites of inflammation...
  54. ncbi request reprint Toll-like receptors 2 and 4 activate STAT1 serine phosphorylation by distinct mechanisms in macrophages
    Sang Hoon Rhee
    The Pulmonary Center, Boston University School of Medicine, Boston, Massachusetts 02118, USA
    J Biol Chem 278:22506-12. 2003
    ..Furthermore, p38 mitogen-activated protein kinase, protein kinase C-delta, and a novel TLR2-specific signaling pathway appear to be necessary to induce Ser-727 STAT1 phosphorylation...
  55. ncbi request reprint Proteasome-mediated regulation of CpG DNA- and peptidoglycan-induced cytokines, inflammatory genes, and mitogen-activated protein kinase activation
    Jing Shen
    Department of Basic Medical Science, School of Medicine, and Shock Trauma Research Center, University of Missouri Kansas City, Kansas City, MO 64108, USA
    Shock 25:594-9. 2006
    ..Collectively, these data strongly support the conclusion that the proteasome is a key regulator of the CpG DNA- and PG-induced signaling pathways...
  56. ncbi request reprint Induction of early inflammatory gene expression in a murine model of nonresuscitated, fixed-volume hemorrhage
    Michael Rajnik
    Department of Pediatrics, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814 4799, USA
    Shock 17:322-8. 2002
    ....
  57. ncbi request reprint Regulation of gene expression in mouse macrophages stimulated with bacterial CpG-DNA and lipopolysaccharide
    Jian Jun Gao
    Department of Basic Medical Sciences, University of Missouri Kansas City, 64108, USA
    J Leukoc Biol 72:1234-45. 2002
    ..We conclude that CpG-DNA signaling through TLR9 activates a subset of genes induced by LPS-TLR4 signaling...
  58. pmc Host immune response to Salmonella enterica serovar Typhimurium infection in mice derived from wild strains
    Giovanna Sebastiani
    Department of Biochemistry, McGill University, Montreal, Quebec, Canada H3G 1A4
    Infect Immun 70:1997-2009. 2002
    ..The high levels of IL-1 expression in the spleens and livers of MOLF/Ei mice may have important implications for the activation of peripheral and central innate immune mechanisms...
  59. ncbi request reprint Toll-like receptor 4 and Toll-IL-1 receptor domain-containing adapter protein (TIRAP)/myeloid differentiation protein 88 adapter-like (Mal) contribute to maximal IL-6 expression in macrophages
    Dagmar Schilling
    Pulmonary Center, School of Medicine, Boston University, Boston, MA 02118, USA
    J Immunol 169:5874-80. 2002
    ..Together, these studies demonstrate that IL-6, like iNOS and IP-10, is differentially expressed in macrophages stimulated via TLR2 vs TLR4, although these differences appear to arise from distinct signaling mechanisms...
  60. ncbi request reprint The proteasome as a lipopolysaccharide-binding protein in macrophages: differential effects of proteasome inhibition on lipopolysaccharide-induced signaling events
    Nilofer Qureshi
    Department of Basic Medical Science, School of Medicine and Shock Trauma Research Center, University of Missouri, Kansas City, MO 64108, USA
    J Immunol 171:1515-25. 2003
    ..These data strongly suggest that the proteasome complex regulates the LPS-induced signal transduction and that it may be an important therapeutic target in Gram-negative sepsis...
  61. ncbi request reprint Bacterial LPS and CpG DNA differentially induce gene expression profiles in mouse macrophages
    Jian Jun Gao
    Department of Basic Medical Sciences, University of Missouri Kansas City, Kansas City, MO 64108, USA
    J Endotoxin Res 9:237-43. 2003
    ..These data indicate that the CpG DNA signaling pathway through TLR9 activates only a subset of genes induced by the LPS TLR4 signaling pathway...
  62. ncbi request reprint Toll-like receptor (TLR)2 and TLR4 agonists regulate CCR expression in human monocytic cells
    Lisa C Parker
    Section of Functional Genomics, Division of Genomic Medicine, University of Sheffield, Sheffield, United Kingdom
    J Immunol 172:4977-86. 2004
    ..This work provides further insights into the mechanisms that regulate leukocyte recruitment and trafficking during TLR-induced inflammatory responses...
  63. ncbi request reprint A novel cell-based system for the rapid quantitative evaluation of (anti)-inflammatory potential of test substances
    Serguei V Kozlov
    Cancer and Developmental Biology Laboratory, National Cancer Institute, P O Box B, Building 539, Frederick, MD 21702, USA
    J Immunol Methods 281:51-63. 2003
    ..In addition, the dual selection capability of the model provides a powerful tool to discover novel molecular components of the NF-kappaB signal transduction pathway...
  64. ncbi request reprint The CATERPILLER protein monarch-1 is an antagonist of toll-like receptor-, tumor necrosis factor alpha-, and Mycobacterium tuberculosis-induced pro-inflammatory signals
    Kristi L Williams
    Lineberger Comprehensive Cancer Center, Division of Infectious Diseases, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    J Biol Chem 280:39914-24. 2005
    ..Mutants containing the NBD-LRR or PyD-NBD also blocked IRAK-1 activation. This is the first example of a CLR protein that antagonizes inflammatory responses initiated by TLR agonists via interference with IRAK-1 activation...
  65. ncbi request reprint Toll-like receptors in health and disease: complex questions remain
    Ian Sabroe
    Division of Genomic Medicine, University of Sheffield, Royal Hallamshire Hospital, Sheffield, United Kingdom
    J Immunol 171:1630-5. 2003
  66. pmc Negative regulation of Toll-like receptor 4 signaling by the Toll-like receptor homolog RP105
    Senad Divanovic
    Division of Molecular Immunology, Cincinnati Children s Hospital Medical Center and University of Cincinnati College of Medicine, Cincinnati, Ohio 45229, USA
    Nat Immunol 6:571-8. 2005
    ..Finally, RP105 regulated TLR4 signaling in dendritic cells as well as endotoxin responses in vivo. Thus, our results identify RP105 as a physiological negative regulator of TLR4 responses...
  67. ncbi request reprint TLR2 and TLR4 serve distinct roles in the host immune response against Mycobacterium bovis BCG
    Kurt A Heldwein
    The Pulmonary Center, Department of Medicine, Boston University School of Medicine, Massachusetts, USA
    J Leukoc Biol 74:277-86. 2003
    ..Thus, TLR2 and TLR4 appear to regulate distinct aspects of the host immune response against BCG...
  68. pmc An angiogenic switch in macrophages involving synergy between Toll-like receptors 2, 4, 7, and 9 and adenosine A(2A) receptors
    Grace Pinhal-Enfield
    Department of Cell Biology and Molecular Medicine, New Jersey Medical School, UMDNJ, Newark, New Jersey 07013, USA
    Am J Pathol 163:711-21. 2003
    ..This angiogenic switch may play an important role in ischemia when TLR agonists are present, providing an interface between innate immunity and wound healing...
  69. ncbi request reprint Key inflammatory signaling pathways are regulated by the proteasome
    Jing Shen
    Department of Basic Medical Science, School of Medicine, and Shock Trauma Research Center, University of Missouri, Kansas City, MO 64108, USA
    Shock 25:472-84. 2006
    ..This represents a novel approach to the identification of signaling pathways that are both toll-like receptor 4- and proteasome-dependent and may lead to the development of new drug targets in Gram-negative sepsis and septic shock...
  70. ncbi request reprint Role of the phosphatidylinositol 3 kinase-Akt pathway in the regulation of IL-10 and IL-12 by Porphyromonas gingivalis lipopolysaccharide
    Michael Martin
    Department of Microbiology, University of Alabama, Birmingham, AL 35294, USA
    J Immunol 171:717-25. 2003
    ..gingivalis LPS affects the induction of key immunoregulatory cytokines that control both qualitative and quantitative aspects of innate and adaptive immunity...
  71. pmc Toll-like receptor 2 is required for inflammatory responses to Francisella tularensis LVS
    Jannet Katz
    Department of Pediatric Dentistry, University of Alabama at Birmingham, Birmingham, AL 35294, USA
    Infect Immun 74:2809-16. 2006
    ..Taken together, our results demonstrate the role of TLR2/TLR6 in the host's inflammatory response to F. tularensis LVS in vitro and the regulatory function of NF-kappaB in modulating the inflammatory response...
  72. ncbi request reprint Inhibition of TLR-4/MD-2 signaling by RP105/MD-1
    Senad Divanovic
    Division of Molecular Immunology, Cincinnati Children s Hospital Medical Center and University of Cincinnati College of Medicine, Cincinnati, Ohio, USA
    J Endotoxin Res 11:363-8. 2005
    ....
  73. ncbi request reprint CpG DNA induced IL-12 p40 gene activation is independent of STAT1 activation or production of interferon consensus sequence binding protein
    Mary Bradford
    Department of Veterans Affairs Medical Center, University of Iowa, College of Medicine, Iowa City, Iowa 52246, USA
    J Biomed Sci 9:688-96. 2002
    ..Thus, CpG DNA-induced IL-12 p40 secretion is mediated by one or more signaling elements distinct from those induced by either LPS or IFN-gamma...
  74. doi request reprint Measurement of Fc gamma receptor-mediated binding and phagocytosis
    Alexander D Politis
    National Institute of Health, Bethesda, Maryland, USA
    Curr Protoc Immunol . 2005
    ..A choice of opsonin in both methods allows for some discrimination between subclasses of FcgammaRs that are responsible for the observed results...
  75. pmc Flagellin of enteropathogenic Escherichia coli stimulates interleukin-8 production in T84 cells
    Xin Zhou
    Center for Vaccine Development, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    Infect Immun 71:2120-9. 2003
    ..Our data clearly indicate that FliC of EPEC is sufficient to induce IL-8 release in T84 cells and that activation of the Erk and p38 pathways is required for IL-8 induction...
  76. ncbi request reprint The IFN-inducible GTPase LRG47 (Irgm1) negatively regulates TLR4-triggered proinflammatory cytokine production and prevents endotoxemia
    Andre Bafica
    Immunobiology Section, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Immunol 179:5514-22. 2007
    ..Thus, our findings reveal a new host-protective function for this GTPase in the response to pathogenic encounter...
  77. ncbi request reprint Pivotal advance: activation of cell surface Toll-like receptors causes shedding of the hemoglobin scavenger receptor CD163
    Lehn K Weaver
    Department of Physiology, Dartmouth Medical School, 1 Medical Center Drive, Lebanon, NH 03756, USA
    J Leukoc Biol 80:26-35. 2006
    ..These data demonstrate that the soluble HbSR is released from monocytic cells in response to TLR signaling as an acute innate immune response to extracellular pathogen infections...

Research Grants15

  1. DIFFERENTIATIVE SIGNALS FOR MACROPHAGE ACTIVATION
    Stefanie N Vogel; Fiscal Year: 2010
    ..Our findings will lead to development of new drugs that will increase the capacity of macrophages to kill bacteria and viruses more effectively or to prevent or repair damage to tissues caused by bacterial or viral infection. ..
  2. DIFFERENTIATIVE SIGNALS FOR MACROPHAGE ACTIVATION
    Stefanie Vogel; Fiscal Year: 2007
    ..abstract_text> ..
  3. Mechanisms and Consequences of Toll Like Receptor Signal Transduction
    Stefanie Vogel; Fiscal Year: 2007
    ..3) Assess the mechanism of TLR tyrosine phosphorylation and its functional consequences. ..
  4. ROLES OF TOLL LIKE RECEPTORS IN INNATE IMMUNITY
    Stefanie Vogel; Fiscal Year: 2005
    ....
  5. EARLY ENDOTOXIN TOLERANCE: CELLULAR/MOLECULAR MECHANISMS
    Stefanie Vogel; Fiscal Year: 2004
    ..It is expected that, at the conclusion of this research, the mechanism of endotoxin tolerance and its potential impact on disease outcome in both sepsis and other diseases will be better understood. ..
  6. DIFFERENTIATIVE SIGNALS FOR MACROPHAGE ACTIVATION
    Stefanie N Vogel; Fiscal Year: 2010
    ..Our findings will lead to development of new drugs that will increase the capacity of macrophages to kill bacteria and viruses more effectively or to prevent or repair damage to tissues caused by bacterial or viral infection. ..