Research Topics
| RONALD VIOLASummaryAffiliation: University of Toledo Country: USA Publications
Research Grants
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Detail Information
Publications
The central enzymes of the aspartate family of amino acid biosynthesisR E Viola
Department of Chemistry, University of Toledo, Toledo, Ohio 43606, USA
Acc Chem Res 34:339-49. 2001..Our current state of knowledge of these enzymes is reviewed, including recently determined structural information and newly constructed bifunctional fusion enzymes...
The impact of structural biology on neurobiologyRonald E Viola
Department of Chemistry, University of Toledo, Toledo, OH 43606, USA
Proc Natl Acad Sci U S A 104:399-400. 2007
The structure of a redundant enzyme: a second isoform of aspartate beta-semialdehyde dehydrogenase in Vibrio choleraeRonald E Viola
Department of Chemistry, The University of Toledo, Toledo, Ohio 43606, USA
Acta Crystallogr D Biol Crystallogr 64:321-30. 2008..cholerae most closely resembles the ASADH from a Gram-positive organism and is likely to bind the coenzyme in a different conformation to that observed in the other V. cholerae isoform...- Purification and preliminary characterization of brain aspartoacylaseRoger A Moore
Department of Chemistry, University of Toledo, 2801 W Bancroft Street, OH 43606, USA
Arch Biochem Biophys 413:1-8. 2003..The clinically relevant E285A mutant reveals an altered enzyme with poor stability and barely detectable activity, while a more conservative E285D substitution leads to only fivefold lower activity than native aspartoacylase... - The role of substrate-binding groups in the mechanism of aspartate-beta-semialdehyde dehydrogenaseJulio Blanco
Department of Chemistry, University of Toledo, Ohio 43606, USA
Acta Crystallogr D Biol Crystallogr 60:1388-95. 2004..1% native activity)... - Examination of the mechanism of human brain aspartoacylase through the binding of an intermediate analogueJohanne Le Coq
Department of Chemistry, University of Toledo, Toledo, Ohio 43606, USA
Biochemistry 47:3484-92. 2008.... - The effect of deuteration on protein structure: a high-resolution comparison of hydrogenous and perdeuterated haloalkane dehalogenaseXuying Liu
Department of Chemistry, University of Toledo, Toledo, OH 43606, USA
Acta Crystallogr D Biol Crystallogr 63:1000-8. 2007..These results underline the importance of carefully verifying the assumption that isotopic substitution does not produce significant structural changes in protein structures... - Structural asymmetry and intersubunit communication in muscle creatine kinaseJeffrey F Ohren
Department of Chemistry, The University of Toledo, Toledo, Ohio 43606, USA
Acta Crystallogr D Biol Crystallogr 63:381-9. 2007..This study provides support for a structural role for the amino-terminus in subunit association and a mechanistic role in active-site communication and catalytic regulation... - Expansion of the aspartate beta-semialdehyde dehydrogenase family: the first structure of a fungal orthologBuenafe T Arachea
Department of Chemistry, University of Toledo, Toledo, Ohio 43606, USA
Acta Crystallogr D Biol Crystallogr 66:205-12. 2010..The detailed functional information derived from this new structure will allow an assessment of ASADH as a possible target for antifungal drug development... - Examination of key intermediates in the catalytic cycle of aspartate-beta-semialdehyde dehydrogenase from a gram-positive infectious bacteriaChristopher R Faehnle
Department of Chemistry, University of Toledo, Toledo, Ohio 43606, USA
J Biol Chem 281:31031-40. 2006..The covalent acyl-enzyme intermediate was trapped by soaking the substrate into crystals of the coenzyme complex, and the structure of this elusive intermediate provides detailed insights into the catalytic mechanism... - A new branch in the family: structure of aspartate-beta-semialdehyde dehydrogenase from Methanococcus jannaschiiChristopher R Faehnle
Department of Chemistry, University of Toledo, 2801 W Bancroft St, Toledo, OH 43606, USA
J Mol Biol 353:1055-68. 2005.... - Structural basis for discrimination between oxyanion substrates or inhibitors in aspartate-beta-semialdehyde dehydrogenaseChristopher R Faehnle
Department of Chemistry, University of Toledo, Ohio 43606, USA
Acta Crystallogr D Biol Crystallogr 60:2320-4. 2004..This subtle change appears to be the difference between a substrate and an inhibitor of this enzyme... - Structural characterization of tartrate dehydrogenase: a versatile enzyme catalyzing multiple reactionsRadhika Malik
Department of Chemistry, University of Toledo, Toledo, Ohio 43606, USA
Acta Crystallogr D Biol Crystallogr 66:673-84. 2010..Each substrate undergoes the initial hydride transfer, but differences in substrate orientation are proposed to account for the different reactions catalyzed by TDH... - Purification, crystallization and preliminary X-ray analysis of aspartokinase III from Escherichia coliJulio Blanco
Department of Chemistry, University of Toledo, 2801 West Bancroft Street, Toledo, Ohio 43606, USA
Acta Crystallogr D Biol Crystallogr 58:352-4. 2002..44, b = 190.31, c = 99.55 A, and data 99.3% complete to 2.7 A. Solving the structure of AK III will provide the first structure of an aspartokinase from any organism... - Capture of an intermediate in the catalytic cycle of L-aspartate-beta-semialdehyde dehydrogenaseJulio Blanco
Department of Chemistry, University of Toledo, Toledo, OH 43606, USA
Proc Natl Acad Sci U S A 100:12613-7. 2003..This pocket, reminiscent of the oxyanion hole found in serine proteases, is completed through hydrogen bonding to the bound phosphate substrate... - A structural basis for the mechanism of aspartate-beta-semialdehyde dehydrogenase from Vibrio choleraeJulio Blanco
University of Toledo, Department of Chemistry, Toledo, OH 43606, USA
Protein Sci 12:27-33. 2003..The conformational changes that do occur result primarily from NADP binding, and are localized to the repositioning of two surface loops located on the rim at opposite sides of the NADP cleft... - Fully automated protein purificationDeMarco V Camper
Department of Chemistry, University of Toledo, Toledo, OH 43606, USA
Anal Biochem 393:176-81. 2009..These individual methods are designed to be coupled and run sequentially in any order to achieve a flexible and fully automated protein purification protocol... - A missense mutation causes aspartase deficiency in Yersinia pestisRonald E Viola
Department of Chemistry, University of Toledo, 2801 W Bancroft Street, Toledo, OH 43606, USA
Microbiology 154:1271-80. 2008..These observations have important implications for understanding the nature of the stringent low-calcium response of Y. pestis and its role in promoting acute disease... - The structural basis for allosteric inhibition of a threonine-sensitive aspartokinaseXuying Liu
Department of Chemistry, University of Toledo, Toledo, Ohio 43606
J Biol Chem 283:16216-25. 2008.... - Crystal structure of F65A/Y131C-methylimidazole carbonic anhydrase V reveals architectural features of an engineered proton shuttleKevin M Jude
Roy and Diana Vagelos Laboratories, Department of Chemistry, University of Pennsylvania, Philadelphia, Pennsylvania 19104 6323, USA
Biochemistry 41:2485-91. 2002.... - Production and characterization of bifunctional enzymes. Substrate channeling in the aspartate pathwayCindy L James
Department of Chemistry, University of Toledo, Toledo, Ohio 43606, USA
Biochemistry 41:3726-31. 2002..coli and suggest that ASADH may provide a bridge to channel the intermediates between the non-consecutive reactions of AK-HDH I... - Inhibitor coordination interactions in the binuclear manganese cluster of arginaseEvis Cama
Roy and Diana Vagelos Laboratories, Department of Chemistry, University of Pennsylvania, Philadelphia, Pennsylvania 19104 6323, USA
Biochemistry 43:8987-99. 2004.... - Expression and purification of aspartate beta-semialdehyde dehydrogenase from infectious microorganismsRoger A Moore
Department of Chemistry, University of Toledo, Toledo, Ohio 43606, USA
Protein Expr Purif 25:189-94. 2002..Kinetic parameters have been determined for each purified enzyme, and the values have been compared to those of E. coli ASA DH... - The initial step in the archaeal aspartate biosynthetic pathway catalyzed by a monofunctional aspartokinaseChristopher R Faehnle
Department of Chemistry, University of Toledo, Toledo, Ohio 43606, USA
Acta Crystallogr Sect F Struct Biol Cryst Commun 62:962-6. 2006..The active-site functional groups responsible for substrate binding and specificity have been identified and roles have been proposed for putative catalytic functional groups... - Characterization of human aspartoacylase: the brain enzyme responsible for Canavan diseaseJohanne Le Coq
Department of Chemistry, University of Toledo, Toledo, Ohio 43606, USA
Biochemistry 45:5878-84. 2006..Chelation studies to remove the zinc result in a reversible loss of catalytic activity, thus establishing aspartoacylase as a zinc metalloenzyme... - Critical catalytic functional groups in the mechanism of aspartate-beta-semialdehyde dehydrogenaseJulio Blanco
Department of Chemistry, University of Toledo, Toledo, Ohio 43606, USA
Acta Crystallogr D Biol Crystallogr 60:1808-15. 2004..However, small perturbations in the positioning of essential catalytic groups or reactive intermediates have dramatic effects on catalytic efficiency... - Production and characterization of bifunctional enzymes. Domain swapping to produce new bifunctional enzymes in the aspartate pathwayCindy L James
Department of Chemistry, University of Toledo, Toledo, Ohio 43606, USA
Biochemistry 41:3720-5. 2002..The more stable aspartokinase III is further stabilized against thermal denaturation in the hybrid bifunctional enzyme and was found to retain some catalytic activity even at temperatures approaching 100 degrees C... - Splicing of unnatural amino acids into proteins: a peptide model studyShoufa Han
University of Toledo, Department of Chemistry, Toledo, Ohio 43606, USA
Protein Pept Lett 11:107-14. 2004..These reactions suggest an approach for the incorporation of unnatural amino acids into proteins by successive native chemical ligation and Staudinger ligation... - Synthesis and evaluation of alternative substrates for arginaseShoufa Han
Department of Chemistry, University of Toledo, Toledo, OH 43606, USA
Bioorg Chem 30:81-94. 2002..Isothiourea homologs previously reported to be nitric oxide synthase inhibitors have been found to undergo a rapid non-enzymatic rearrangement to a species that is probably the true inhibitor...
Research Grants
- Probing the Enzymatic Basis of Canavan DiseaseRONALD VIOLA; Fiscal Year: 2009..We will also learn how aspartoacylase functions, and why it malfunctions in Canavan disease. ..
- Development of Aspartate Pathway Inhibitors as Novel AntibioticsRONALD EDWARD VIOLA; Fiscal Year: 2010....