RONALD VIOLA

Summary

Affiliation: University of Toledo
Country: USA

Publications

  1. ncbi request reprint The central enzymes of the aspartate family of amino acid biosynthesis
    R E Viola
    Department of Chemistry, University of Toledo, Toledo, Ohio 43606, USA
    Acc Chem Res 34:339-49. 2001
  2. pmc The impact of structural biology on neurobiology
    Ronald E Viola
    Department of Chemistry, University of Toledo, Toledo, OH 43606, USA
    Proc Natl Acad Sci U S A 104:399-400. 2007
  3. doi request reprint The structure of a redundant enzyme: a second isoform of aspartate beta-semialdehyde dehydrogenase in Vibrio cholerae
    Ronald E Viola
    Department of Chemistry, The University of Toledo, Toledo, Ohio 43606, USA
    Acta Crystallogr D Biol Crystallogr 64:321-30. 2008
  4. ncbi request reprint Purification and preliminary characterization of brain aspartoacylase
    Roger A Moore
    Department of Chemistry, University of Toledo, 2801 W Bancroft Street, OH 43606, USA
    Arch Biochem Biophys 413:1-8. 2003
  5. ncbi request reprint The role of substrate-binding groups in the mechanism of aspartate-beta-semialdehyde dehydrogenase
    Julio Blanco
    Department of Chemistry, University of Toledo, Ohio 43606, USA
    Acta Crystallogr D Biol Crystallogr 60:1388-95. 2004
  6. pmc Examination of the mechanism of human brain aspartoacylase through the binding of an intermediate analogue
    Johanne Le Coq
    Department of Chemistry, University of Toledo, Toledo, Ohio 43606, USA
    Biochemistry 47:3484-92. 2008
  7. ncbi request reprint The effect of deuteration on protein structure: a high-resolution comparison of hydrogenous and perdeuterated haloalkane dehalogenase
    Xuying Liu
    Department of Chemistry, University of Toledo, Toledo, OH 43606, USA
    Acta Crystallogr D Biol Crystallogr 63:1000-8. 2007
  8. ncbi request reprint Structural asymmetry and intersubunit communication in muscle creatine kinase
    Jeffrey F Ohren
    Department of Chemistry, The University of Toledo, Toledo, Ohio 43606, USA
    Acta Crystallogr D Biol Crystallogr 63:381-9. 2007
  9. doi request reprint Expansion of the aspartate beta-semialdehyde dehydrogenase family: the first structure of a fungal ortholog
    Buenafe T Arachea
    Department of Chemistry, University of Toledo, Toledo, Ohio 43606, USA
    Acta Crystallogr D Biol Crystallogr 66:205-12. 2010
  10. ncbi request reprint Examination of key intermediates in the catalytic cycle of aspartate-beta-semialdehyde dehydrogenase from a gram-positive infectious bacteria
    Christopher R Faehnle
    Department of Chemistry, University of Toledo, Toledo, Ohio 43606, USA
    J Biol Chem 281:31031-40. 2006

Research Grants

Collaborators

  • David Christianson
  • Daniel Mansuy
  • B Leif Hanson
  • Robert R Brubaker
  • PAUL A LANGAN
  • Christopher R Faehnle
  • Julio Blanco
  • Xuying Liu
  • Roger A Moore
  • Johanne Le Coq
  • Shoufa Han
  • Radhika Malik
  • Jeffrey F Ohren
  • Cindy L James
  • Buenafe T Arachea
  • DeMarco V Camper
  • Alexander G Pavlovsky
  • Alexander Pavlovsky
  • Evis Cama
  • Kevin M Jude
  • Chengfu Xu
  • Ruslan Sanishvili
  • Melisa L Kundracik
  • Charles L Borders
  • Paul Edmiston
  • Carlito Lebrilla
  • Hyun Joo An
  • Jean Luc Boucher
  • David E Ash
  • David M Coe
  • Frances A Emig
  • Stephanie Pethe
  • Venkataraman Kabaleeswaran
  • David N Silverman
  • William E Bocik
  • Chingkuang Tu
  • S Kirk Wright

Detail Information

Publications29

  1. ncbi request reprint The central enzymes of the aspartate family of amino acid biosynthesis
    R E Viola
    Department of Chemistry, University of Toledo, Toledo, Ohio 43606, USA
    Acc Chem Res 34:339-49. 2001
    ..Our current state of knowledge of these enzymes is reviewed, including recently determined structural information and newly constructed bifunctional fusion enzymes...
  2. pmc The impact of structural biology on neurobiology
    Ronald E Viola
    Department of Chemistry, University of Toledo, Toledo, OH 43606, USA
    Proc Natl Acad Sci U S A 104:399-400. 2007
  3. doi request reprint The structure of a redundant enzyme: a second isoform of aspartate beta-semialdehyde dehydrogenase in Vibrio cholerae
    Ronald E Viola
    Department of Chemistry, The University of Toledo, Toledo, Ohio 43606, USA
    Acta Crystallogr D Biol Crystallogr 64:321-30. 2008
    ..cholerae most closely resembles the ASADH from a Gram-positive organism and is likely to bind the coenzyme in a different conformation to that observed in the other V. cholerae isoform...
  4. ncbi request reprint Purification and preliminary characterization of brain aspartoacylase
    Roger A Moore
    Department of Chemistry, University of Toledo, 2801 W Bancroft Street, OH 43606, USA
    Arch Biochem Biophys 413:1-8. 2003
    ..The clinically relevant E285A mutant reveals an altered enzyme with poor stability and barely detectable activity, while a more conservative E285D substitution leads to only fivefold lower activity than native aspartoacylase...
  5. ncbi request reprint The role of substrate-binding groups in the mechanism of aspartate-beta-semialdehyde dehydrogenase
    Julio Blanco
    Department of Chemistry, University of Toledo, Ohio 43606, USA
    Acta Crystallogr D Biol Crystallogr 60:1388-95. 2004
    ..1% native activity)...
  6. pmc Examination of the mechanism of human brain aspartoacylase through the binding of an intermediate analogue
    Johanne Le Coq
    Department of Chemistry, University of Toledo, Toledo, Ohio 43606, USA
    Biochemistry 47:3484-92. 2008
    ....
  7. ncbi request reprint The effect of deuteration on protein structure: a high-resolution comparison of hydrogenous and perdeuterated haloalkane dehalogenase
    Xuying Liu
    Department of Chemistry, University of Toledo, Toledo, OH 43606, USA
    Acta Crystallogr D Biol Crystallogr 63:1000-8. 2007
    ..These results underline the importance of carefully verifying the assumption that isotopic substitution does not produce significant structural changes in protein structures...
  8. ncbi request reprint Structural asymmetry and intersubunit communication in muscle creatine kinase
    Jeffrey F Ohren
    Department of Chemistry, The University of Toledo, Toledo, Ohio 43606, USA
    Acta Crystallogr D Biol Crystallogr 63:381-9. 2007
    ..This study provides support for a structural role for the amino-terminus in subunit association and a mechanistic role in active-site communication and catalytic regulation...
  9. doi request reprint Expansion of the aspartate beta-semialdehyde dehydrogenase family: the first structure of a fungal ortholog
    Buenafe T Arachea
    Department of Chemistry, University of Toledo, Toledo, Ohio 43606, USA
    Acta Crystallogr D Biol Crystallogr 66:205-12. 2010
    ..The detailed functional information derived from this new structure will allow an assessment of ASADH as a possible target for antifungal drug development...
  10. ncbi request reprint Examination of key intermediates in the catalytic cycle of aspartate-beta-semialdehyde dehydrogenase from a gram-positive infectious bacteria
    Christopher R Faehnle
    Department of Chemistry, University of Toledo, Toledo, Ohio 43606, USA
    J Biol Chem 281:31031-40. 2006
    ..The covalent acyl-enzyme intermediate was trapped by soaking the substrate into crystals of the coenzyme complex, and the structure of this elusive intermediate provides detailed insights into the catalytic mechanism...
  11. ncbi request reprint A new branch in the family: structure of aspartate-beta-semialdehyde dehydrogenase from Methanococcus jannaschii
    Christopher R Faehnle
    Department of Chemistry, University of Toledo, 2801 W Bancroft St, Toledo, OH 43606, USA
    J Mol Biol 353:1055-68. 2005
    ....
  12. ncbi request reprint Structural basis for discrimination between oxyanion substrates or inhibitors in aspartate-beta-semialdehyde dehydrogenase
    Christopher R Faehnle
    Department of Chemistry, University of Toledo, Ohio 43606, USA
    Acta Crystallogr D Biol Crystallogr 60:2320-4. 2004
    ..This subtle change appears to be the difference between a substrate and an inhibitor of this enzyme...
  13. pmc Structural characterization of tartrate dehydrogenase: a versatile enzyme catalyzing multiple reactions
    Radhika Malik
    Department of Chemistry, University of Toledo, Toledo, Ohio 43606, USA
    Acta Crystallogr D Biol Crystallogr 66:673-84. 2010
    ..Each substrate undergoes the initial hydride transfer, but differences in substrate orientation are proposed to account for the different reactions catalyzed by TDH...
  14. ncbi request reprint Purification, crystallization and preliminary X-ray analysis of aspartokinase III from Escherichia coli
    Julio Blanco
    Department of Chemistry, University of Toledo, 2801 West Bancroft Street, Toledo, Ohio 43606, USA
    Acta Crystallogr D Biol Crystallogr 58:352-4. 2002
    ..44, b = 190.31, c = 99.55 A, and data 99.3% complete to 2.7 A. Solving the structure of AK III will provide the first structure of an aspartokinase from any organism...
  15. pmc Capture of an intermediate in the catalytic cycle of L-aspartate-beta-semialdehyde dehydrogenase
    Julio Blanco
    Department of Chemistry, University of Toledo, Toledo, OH 43606, USA
    Proc Natl Acad Sci U S A 100:12613-7. 2003
    ..This pocket, reminiscent of the oxyanion hole found in serine proteases, is completed through hydrogen bonding to the bound phosphate substrate...
  16. pmc A structural basis for the mechanism of aspartate-beta-semialdehyde dehydrogenase from Vibrio cholerae
    Julio Blanco
    University of Toledo, Department of Chemistry, Toledo, OH 43606, USA
    Protein Sci 12:27-33. 2003
    ..The conformational changes that do occur result primarily from NADP binding, and are localized to the repositioning of two surface loops located on the rim at opposite sides of the NADP cleft...
  17. pmc Fully automated protein purification
    DeMarco V Camper
    Department of Chemistry, University of Toledo, Toledo, OH 43606, USA
    Anal Biochem 393:176-81. 2009
    ..These individual methods are designed to be coupled and run sequentially in any order to achieve a flexible and fully automated protein purification protocol...
  18. doi request reprint A missense mutation causes aspartase deficiency in Yersinia pestis
    Ronald E Viola
    Department of Chemistry, University of Toledo, 2801 W Bancroft Street, Toledo, OH 43606, USA
    Microbiology 154:1271-80. 2008
    ..These observations have important implications for understanding the nature of the stringent low-calcium response of Y. pestis and its role in promoting acute disease...
  19. pmc The structural basis for allosteric inhibition of a threonine-sensitive aspartokinase
    Xuying Liu
    Department of Chemistry, University of Toledo, Toledo, Ohio 43606
    J Biol Chem 283:16216-25. 2008
    ....
  20. ncbi request reprint Crystal structure of F65A/Y131C-methylimidazole carbonic anhydrase V reveals architectural features of an engineered proton shuttle
    Kevin M Jude
    Roy and Diana Vagelos Laboratories, Department of Chemistry, University of Pennsylvania, Philadelphia, Pennsylvania 19104 6323, USA
    Biochemistry 41:2485-91. 2002
    ....
  21. ncbi request reprint Production and characterization of bifunctional enzymes. Substrate channeling in the aspartate pathway
    Cindy L James
    Department of Chemistry, University of Toledo, Toledo, Ohio 43606, USA
    Biochemistry 41:3726-31. 2002
    ..coli and suggest that ASADH may provide a bridge to channel the intermediates between the non-consecutive reactions of AK-HDH I...
  22. ncbi request reprint Inhibitor coordination interactions in the binuclear manganese cluster of arginase
    Evis Cama
    Roy and Diana Vagelos Laboratories, Department of Chemistry, University of Pennsylvania, Philadelphia, Pennsylvania 19104 6323, USA
    Biochemistry 43:8987-99. 2004
    ....
  23. ncbi request reprint Expression and purification of aspartate beta-semialdehyde dehydrogenase from infectious microorganisms
    Roger A Moore
    Department of Chemistry, University of Toledo, Toledo, Ohio 43606, USA
    Protein Expr Purif 25:189-94. 2002
    ..Kinetic parameters have been determined for each purified enzyme, and the values have been compared to those of E. coli ASA DH...
  24. pmc The initial step in the archaeal aspartate biosynthetic pathway catalyzed by a monofunctional aspartokinase
    Christopher R Faehnle
    Department of Chemistry, University of Toledo, Toledo, Ohio 43606, USA
    Acta Crystallogr Sect F Struct Biol Cryst Commun 62:962-6. 2006
    ..The active-site functional groups responsible for substrate binding and specificity have been identified and roles have been proposed for putative catalytic functional groups...
  25. pmc Characterization of human aspartoacylase: the brain enzyme responsible for Canavan disease
    Johanne Le Coq
    Department of Chemistry, University of Toledo, Toledo, Ohio 43606, USA
    Biochemistry 45:5878-84. 2006
    ..Chelation studies to remove the zinc result in a reversible loss of catalytic activity, thus establishing aspartoacylase as a zinc metalloenzyme...
  26. ncbi request reprint Critical catalytic functional groups in the mechanism of aspartate-beta-semialdehyde dehydrogenase
    Julio Blanco
    Department of Chemistry, University of Toledo, Toledo, Ohio 43606, USA
    Acta Crystallogr D Biol Crystallogr 60:1808-15. 2004
    ..However, small perturbations in the positioning of essential catalytic groups or reactive intermediates have dramatic effects on catalytic efficiency...
  27. ncbi request reprint Production and characterization of bifunctional enzymes. Domain swapping to produce new bifunctional enzymes in the aspartate pathway
    Cindy L James
    Department of Chemistry, University of Toledo, Toledo, Ohio 43606, USA
    Biochemistry 41:3720-5. 2002
    ..The more stable aspartokinase III is further stabilized against thermal denaturation in the hybrid bifunctional enzyme and was found to retain some catalytic activity even at temperatures approaching 100 degrees C...
  28. ncbi request reprint Splicing of unnatural amino acids into proteins: a peptide model study
    Shoufa Han
    University of Toledo, Department of Chemistry, Toledo, Ohio 43606, USA
    Protein Pept Lett 11:107-14. 2004
    ..These reactions suggest an approach for the incorporation of unnatural amino acids into proteins by successive native chemical ligation and Staudinger ligation...
  29. ncbi request reprint Synthesis and evaluation of alternative substrates for arginase
    Shoufa Han
    Department of Chemistry, University of Toledo, Toledo, OH 43606, USA
    Bioorg Chem 30:81-94. 2002
    ..Isothiourea homologs previously reported to be nitric oxide synthase inhibitors have been found to undergo a rapid non-enzymatic rearrangement to a species that is probably the true inhibitor...

Research Grants7

  1. Probing the Enzymatic Basis of Canavan Disease
    RONALD VIOLA; Fiscal Year: 2009
    ..We will also learn how aspartoacylase functions, and why it malfunctions in Canavan disease. ..
  2. Development of Aspartate Pathway Inhibitors as Novel Antibiotics
    RONALD EDWARD VIOLA; Fiscal Year: 2010
    ....