Sergei Vinogradov

Summary

Affiliation: University of Pennsylvania
Country: USA

Publications

  1. ncbi request reprint A method for measuring oxygen distributions in tissue using frequency domain phosphorometry
    Sergei A Vinogradov
    Department of Biochemistry and Biophysics, School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
    Comp Biochem Physiol A Mol Integr Physiol 132:147-52. 2002
  2. pmc Oxygen distribution and vascular injury in the mouse eye measured by phosphorescence-lifetime imaging
    David F Wilson
    Department of Biochemistry, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Appl Opt 44:5239-48. 2005
  3. pmc Two-photon high-resolution measurement of partial pressure of oxygen in cerebral vasculature and tissue
    Sava Sakadzic
    Photon Migration Imaging Laboratory, Athinoula A Martinos Center for Biomedical Imaging, Department of Radiology, Massachusetts General Hospital and Harvard Medical School, Charlestown, MA, USA
    Nat Methods 7:755-9. 2010
  4. pmc Pi-extended dipyrrins capable of highly fluorogenic complexation with metal ions
    Mikhail A Filatov
    Department of Chemistry, Moscow State University, 119899 Moscow, Russia
    J Am Chem Soc 132:9552-4. 2010
  5. pmc Noninvasive imaging of the distribution in oxygen in tissue in vivo using near-infrared phosphors
    S A Vinogradov
    Department of Biochemistry and Biophysics, Medical School, University of Pennsylvania, Philadelphia 19104, USA
    Biophys J 70:1609-17. 1996
  6. ncbi request reprint Arylamide dendrimers with flexible linkers via haloacyl halide method
    Sergei A Vinogradov
    Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Org Lett 7:1761-4. 2005
  7. ncbi request reprint Electrostatic core shielding in dendritic polyglutamic porphyrins
    S A Vinogradov
    Department of Biochemistry and Biophysics, School of Medicine, University of Pennsylvania, Philadelphia 19104, USA
    Chemistry 6:2456-61. 2000
  8. ncbi request reprint Porphyrin and tetrabenzoporphyrin dendrimers: tunable membrane-impermeable fluorescent pH nanosensors
    Olga Finikova
    Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    J Am Chem Soc 125:4882-93. 2003
  9. ncbi request reprint Direct observation of triplet state emission of single molecules: single molecule phosphorescence quenching of metalloporphyrin and organometallic complexes by molecular oxygen and their quenching rate distributions
    Erwen Mei
    Department of Chemistry, University of Pennsylvania, Philadelphia, PA 19104, USA
    J Am Chem Soc 125:13198-204. 2003
  10. ncbi request reprint Accessibility of oxygen with respect to the heme pocket in horseradish peroxidase
    Mazdak Khajehpour
    Department of Biochemistry and Biophysics, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Proteins 53:656-66. 2003

Research Grants

Collaborators

  • Vivek J Srinivasan
  • Anna Devor
  • Mazdak Khajehpour
  • S M Evans
  • David Wilson
  • Vadim Soloviev
  • J M Vanderkooi
  • Mahsa Ranji
  • Yoshiko Miura
  • V Percec
  • Britton Chance
  • Olga S Finikova
  • Andrei V Cheprakov
  • Mikhail A Filatov
  • Artem Y Lebedev
  • Sofia V Apreleva
  • Raymond P Briñas
  • Robin M Hochstrasser
  • Sava Sakadzic
  • Mark S Kaucher
  • Sovia V Apreleva
  • Thomas Troxler
  • Lisa S Ziemer
  • Janna Nibbs
  • Patrick J Carroll
  • Vladimir V Rozhkov
  • Erwen Mei
  • Olga Finikova
  • Isolde Dunphy
  • Mohammad A Yaseen
  • Emiri T Mandeville
  • Eng H Lo
  • Svetlana Ruvinskaya
  • Ken Arai
  • Emmanuel Roussakis
  • Sergei N Mukhin
  • David A Boas
  • Andrés E Dulcey
  • Alessandro Senes
  • Danniel A Hammer
  • Anthony J Kim
  • William F Degrado
  • Paul A Heiney
  • Mihai Peterca
  • William M F Lee
  • Chandra Sehgal
  • Sergei E Aleshchenkov
  • Bogumil Zelent
  • Alexander Galkin
  • Vladimir Rozhkov
  • Maria Cordero
  • Cecilia Hägerhäll
  • Sergio Dalosto

Detail Information

Publications35

  1. ncbi request reprint A method for measuring oxygen distributions in tissue using frequency domain phosphorometry
    Sergei A Vinogradov
    Department of Biochemistry and Biophysics, School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
    Comp Biochem Physiol A Mol Integr Physiol 132:147-52. 2002
    ..Here we describe a multi-frequency system for measurements of oxygen distributions in tissue in vivo...
  2. pmc Oxygen distribution and vascular injury in the mouse eye measured by phosphorescence-lifetime imaging
    David F Wilson
    Department of Biochemistry, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Appl Opt 44:5239-48. 2005
    ..High-resolution maps of oxygen distribution in the retina can provide greater understanding of the role of oxygen and vascular function in diseases of the eye...
  3. pmc Two-photon high-resolution measurement of partial pressure of oxygen in cerebral vasculature and tissue
    Sava Sakadzic
    Photon Migration Imaging Laboratory, Athinoula A Martinos Center for Biomedical Imaging, Department of Radiology, Massachusetts General Hospital and Harvard Medical School, Charlestown, MA, USA
    Nat Methods 7:755-9. 2010
    ..The properties of the probe allowed for direct high-resolution measurement of cortical extravascular (tissue) pO(2), opening many possibilities for functional metabolic brain studies...
  4. pmc Pi-extended dipyrrins capable of highly fluorogenic complexation with metal ions
    Mikhail A Filatov
    Department of Chemistry, Moscow State University, 119899 Moscow, Russia
    J Am Chem Soc 132:9552-4. 2010
    ..The metal complexes possess tunable spectral bands and exhibit different emission properties depending on the mode of metal coordination...
  5. pmc Noninvasive imaging of the distribution in oxygen in tissue in vivo using near-infrared phosphors
    S A Vinogradov
    Department of Biochemistry and Biophysics, Medical School, University of Pennsylvania, Philadelphia 19104, USA
    Biophys J 70:1609-17. 1996
    ..In addition, tissue oxygen pressures can be monitored in real time, even through centimeter thicknesses of tissue...
  6. ncbi request reprint Arylamide dendrimers with flexible linkers via haloacyl halide method
    Sergei A Vinogradov
    Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Org Lett 7:1761-4. 2005
    ..The G2 AG dendrons were coupled to a Pd porphyrin core, demonstrating outstanding encapsulation efficiency in aqueous solutions...
  7. ncbi request reprint Electrostatic core shielding in dendritic polyglutamic porphyrins
    S A Vinogradov
    Department of Biochemistry and Biophysics, School of Medicine, University of Pennsylvania, Philadelphia 19104, USA
    Chemistry 6:2456-61. 2000
    ..The "dendrimer-protected" porphyrins are convenient fluorescent pH sensors in the biological pH range...
  8. ncbi request reprint Porphyrin and tetrabenzoporphyrin dendrimers: tunable membrane-impermeable fluorescent pH nanosensors
    Olga Finikova
    Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    J Am Chem Soc 125:4882-93. 2003
    ..When proton channels were created in the liposome walls, both compounds responded equally to the bulk pH changes. These results suggest that porphyrin dendrimers can be used as fluorescent pH indicators for proton gradient measurements...
  9. ncbi request reprint Direct observation of triplet state emission of single molecules: single molecule phosphorescence quenching of metalloporphyrin and organometallic complexes by molecular oxygen and their quenching rate distributions
    Erwen Mei
    Department of Chemistry, University of Pennsylvania, Philadelphia, PA 19104, USA
    J Am Chem Soc 125:13198-204. 2003
    ..The possibility of creating a quantitative single oxygen molecule sensor is suggested...
  10. ncbi request reprint Accessibility of oxygen with respect to the heme pocket in horseradish peroxidase
    Mazdak Khajehpour
    Department of Biochemistry and Biophysics, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Proteins 53:656-66. 2003
    ..We demonstrate that the model of a "fluctuating entry point," as suggested by Zwanzig (J Chem Phys 1992;97:3587-3589), properly describes the diffusion of oxygen through the protein matrix...
  11. ncbi request reprint Oxyphor R2 and G2: phosphors for measuring oxygen by oxygen-dependent quenching of phosphorescence
    Isolde Dunphy
    Department of Biochemistry and Biophysics, School of Medicine, University of Pennsylvania, Philadelphia, PA 19104 6059, USA
    Anal Biochem 310:191-8. 2002
    ..4 to 7.8). In vivo application is demonstrated by using Oxyphor G2 to noninvasively determine the oxygen distribution in a subcutaneous tumor growing in rats...
  12. ncbi request reprint Imaging oxygen pressure in the retina of the mouse eye
    David F Wilson
    Adv Exp Med Biol 566:159-65. 2005
    ....
  13. pmc Feasibility of diffuse optical imaging with long-lived luminescent probes
    Sofia V Apreleva
    Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Opt Lett 31:1082-4. 2006
    ..The approach developed will guide experimental imaging of phosphorescence lifetime and oxygen pressure in living tissue...
  14. ncbi request reprint Oxygen pressures in the interstitial space and their relationship to those in the blood plasma in resting skeletal muscle
    David F Wilson
    Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, PA 19104, USA
    J Appl Physiol (1985) 101:1648-56. 2006
    ..It was, therefore, not further used...
  15. pmc Imaging oxygen pressure in the rodent retina by phosphorescence lifetime
    David F Wilson
    Department of Biochemistry, Medical School, University of Pennsylvania, Philadelphia 19104, USA
    Adv Exp Med Biol 578:119-24. 2006
  16. ncbi request reprint Simultaneous fluorometry and phosphorometry of Langendorff perfused rat heart: ex vivo animal studies
    Mahsa Ranji
    Complex Media Laboratory, Department of Electrical and Systems Engineering, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Opt Lett 31:2995-7. 2006
    ....
  17. pmc Tomographic imaging of oxygen by phosphorescence lifetime
    Sovia V Apreleva
    Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, PA 19104, USA
    Appl Opt 45:8547-59. 2006
    ..We provide what we believe to be the first example of oxygen imaging in three dimensions using long-lived phosphorescent probes and establish the potential of these probes for diffuse optical tomography...
  18. ncbi request reprint Selective transport of water mediated by porous dendritic dipeptides
    Mark S Kaucher
    Roy and Diana Vagelos Laboratories, Department of Chemistry, University of Pennsylvania, Philadelphia, Pennsylvania 19104 6323, USA
    J Am Chem Soc 129:11698-9. 2007
  19. pmc Oxygen pressures in the interstitial space of skeletal muscle and tumors in vivo
    David F Wilson
    Department of Biochemistry and Biophysics, Medical School, University of Pennsylvania, Philadelphia, PA 19104, USA
    Adv Exp Med Biol 614:53-62. 2008
    ..In mice, anesthesia with ketamine plus xylazine markedly decreased oxygen pressures in the interstitial and intravascular spaces compared to awake or isoflurane anesthetized mice...
  20. pmc Phosphorescent oxygen sensor with dendritic protection and two-photon absorbing antenna
    Raymond P Briñas
    Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    J Am Chem Soc 127:11851-62. 2005
    ..Several Pt porphyrin-coumarin based sensors were synthesized and their photophyics studied to evaluate the proposed design...
  21. ncbi request reprint Monitoring the dynamics of tissue oxygenation in vivo by phosphorescence quenching
    David F Wilson
    Department of Biochemistry and Biophysics, School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
    Adv Exp Med Biol 540:1-5. 2003
  22. ncbi request reprint Self-assembly of amphiphilic dendritic dipeptides into helical pores
    Virgil Percec
    Roy and Diana Vagelos Laboratories, Department of Chemistry, University of Pennsylvania, Philadelphia, Pennsylvania 19104 6323, USA
    Nature 430:764-8. 2004
    ..We expect that this class of self-assembling dendrimers will allow the design of a variety of biologically inspired systems with functional properties arising from their porous structure...
  23. ncbi request reprint Oxygen distribution in murine tumors: characterization using oxygen-dependent quenching of phosphorescence
    Lisa S Ziemer
    Department of Clinical Studies, Philadelphia School of Veterinary Medicine, Univ of Pennsylvania, Rm 330, BRB II III, 421 Curie Blvd, Philadelphia, PA 19104, USA
    J Appl Physiol 98:1503-10. 2005
    ..The results show that phosphorescence quenching is a reliable, reproducible, and noninvasive method capable of providing real-time determination of oxygen concentrations within tumors...
  24. ncbi request reprint Measurement of tumor oxygenation using new frequency domain phosphorometers
    David F Wilson
    Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, PA 19104 6059, USA
    Comp Biochem Physiol A Mol Integr Physiol 132:153-9. 2002
    ..This procedure determines the tissue oxygen distribution at each position in the grid. Regions of relative hypoxia (associated with the tumor) can be readily localized and the extent of hypoxia quantitatively evaluated...
  25. ncbi request reprint Oxygen distributions in tissue measured by phosphorescence quenching
    Sergei A Vinogradov
    Dept of Biochem and Biophys, Univer of Pennsylvania, Philadelphia, PA 19104, USA
    Adv Exp Med Biol 510:181-5. 2003
  26. pmc Effects of structural deformations on optical properties of tetrabenzoporphyrins: free-bases and Pd complexes
    Artem Y Lebedev
    Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    J Phys Chem A 112:7723-33. 2008
    ..15; PdPh4TBP: tau(phos) = 258 micros, phi(phos) = 0.08). It appears that pi-extension is capable of counterbalancing deleterious effects of nonplanar deformations on triplet emissivity of Pd porphyrins...
  27. ncbi request reprint Influence of nonplanarity and extended conjugation on porphyrin basicity
    Olga S Finikova
    Department of Chemistry, Moscow State University, Russia
    Inorg Chem 41:6944-6. 2002
    ..Low basicities of distorted Ar(4)TBPs are believed to be due to extended pi-conjugation, which causes delocalization of the core electron density in these porphyrins and reduces negative charges at the protonation site...
  28. pmc Synthesis of 5,15-diaryltetrabenzoporphyrins
    Mikhail A Filatov
    Department of Chemistry, Moscow State University, Moscow 119899, Russia
    J Org Chem 73:4175-85. 2008
    ..Ar 2TBP free bases exhibit optical absorption spectra similar to those of meso-unsubstituted tetrabenzoporphyrins and fluoresce with high quantum yields. Pd complex of Ph 2TBP was found to be highly phosphorescent at room temperature...
  29. pmc Influence of optical heterogeneities on reconstruction of spatial phosphorescence lifetime distributions
    Sofia V Apreleva
    Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Opt Lett 33:782-4. 2008
    ..We computationally show that tomographic PLI can perform equally well in heterogeneous environments relying only on surface measurements of phosphorescence...
  30. ncbi request reprint Luminescent Zn and Pd tetranaphthaloporphyrins
    Vladimir V Rozhkov
    Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Inorg Chem 42:4253-5. 2003
    ..The phosphorescence of 2 occurs with lifetime of about 65 micros and (phi=6.5%) in deoxygenated DMF solution. Metalloporphyrins 1 and 2 are promising near infrared dyes biomedical applications...
  31. pmc Energy and electron transfer in enhanced two-photon-absorbing systems with triplet cores
    Olga S Finikova
    Departments of Biochemistry and Biophysics and Chemistry, University of Pennsylvania, Philadelphia, PA 19104, USA
    J Phys Chem A 111:6977-90. 2007
    ..We also have found that PtTBPs possess unexpectedly strong forbidden S0 --> T1 bands (lambda(max) = 762 nm, epsilon = 120 M-1 cm-1). The latter may overlap with the laser spectrum and lead to unwanted linear excitation...
  32. ncbi request reprint Phosphorescence lifetime imaging in turbid media: the inverse problem and experimental image reconstruction
    Vadim Y Soloviev
    Department of Biochemistry and Biophysics, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Appl Opt 43:564-74. 2004
    ..e., the Tikhonov-Phillips regularization and the maximum entropy method. Theoretical results are experimentally validated, and the reconstructed images of phosphorescent objects rendering oxygen maps in a layer are presented...
  33. ncbi request reprint Flexibility in proteins: tuning the sensitivity to O2 diffusion by varying the lifetime of a phosphorescent sensor in horseradish peroxidase
    Janna Nibbs
    Department of Biochemistry and Biophysics, Johnson Research Foundation, School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
    Photochem Photobiol 80:36-40. 2004
    ..The high quantum yield and suitable lifetime make Pt-porphyrins appropriate as sensors of O(2) diffusion and flexibility in heme proteins...
  34. pmc Synthesis and luminescence of soluble meso-unsubstituted tetrabenzo- and tetranaphtho[2,3]porphyrins
    Olga S Finikova
    Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    J Org Chem 70:9562-72. 2005
    ..Remarkably, the phosphorescence quantum yields of Pd and Pt TBPs reach as high as 20-50%, which places them among the brightest near-infrared phosphors known to date...
  35. ncbi request reprint Synthesis of symmetrical tetraaryltetranaphtho[2,3]porphyrins
    Olga S Finikova
    Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    J Org Chem 70:4617-28. 2005
    ..The X-ray crystallographic data suggest that these spectral shifts are caused not by the differences in nonplanar distortions of the macrocycles but by the purely electronic effects of the substituents...

Research Grants5

  1. DENDRITIC O2 SENSOR WITH TWO-PHOTON ABSORBING ANTENNA
    Sergei Vinogradov; Fiscal Year: 2005
    ..If successful, these experiments will open avenues for novel research, which will span from the studies of protein conformations by small molecule diffusion to the construction of a single-molecule oxygen sensor. ..
  2. Oxygen microscopy by two-photon excited phosphorescence
    Sergei Vinogradov; Fiscal Year: 2007
    ..The biological systems are selected to evaluate the performance of the technique and to simultaneously obtain valuable information for the related bio-medical problems. ..
  3. Oxygen microscopy by two-photon excited phosphorescence
    Sergei Vinogradov; Fiscal Year: 2009
    ..The biological systems are selected to evaluate the performance of the technique and to simultaneously obtain valuable information for the related bio-medical problems. ..
  4. Oxygen microscopy by two-photon excited phosphorescence
    Sergei Vinogradov; Fiscal Year: 2010
    ..The biological systems are selected to evaluate the performance of the technique and to simultaneously obtain valuable information for the related bio-medical problems. ..