Research Topics
| Miguel Vicente-ManzanaresSummaryAffiliation: University of Virginia Country: USA Publications
| Collaborators
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Detail Information
Publications
Regulation of protrusion, adhesion dynamics, and polarity by myosins IIA and IIB in migrating cellsMiguel Vicente-Manzanares
Department of Cell Biology, University of Virginia, Charlottesville, VA 22908
J Cell Biol 176:573-80. 2007..It mediates each of the major component processes that drive migration, e.g., polarization, protrusion, adhesion assembly and turnover, polarity, signaling, and tail retraction, and it integrates spatially separated processes...- Myosin IIA/IIB restrict adhesive and protrusive signaling to generate front-back polarity in migrating cellsMiguel Vicente-Manzanares
Department of Cell Biology, University of Virginia School of Medicine, Charlottesville, VA 22908, USA
J Cell Biol 193:381-96. 2011..These observations lead to a model for front-back polarity through local GEF depletion... - Myosin light chain mono- and di-phosphorylation differentially regulate adhesion and polarity in migrating cellsMiguel Vicente-Manzanares
Department of Cell Biology, University of Virginia, School of Medicine, Charlottesville, VA 22908, USA
Biochem Biophys Res Commun 402:537-42. 2010..Finally, RLC phosphorylation regulates the assembly and stability of MIIB, but not MIIA. Our data reveal a novel mechanism by which the degree of phosphorylation of the RLC differentially controls cell adhesion and polarity... - Actin and alpha-actinin orchestrate the assembly and maturation of nascent adhesions in a myosin II motor-independent mannerColin K Choi
Department of Biomedical Engineering, University of Virginia, Charlottesville, Virginia 22908, USA
Nat Cell Biol 10:1039-50. 2008..From these studies, we have developed a model for adhesion assembly that clarifies the relative contributions of myosin II and actin polymerization and organization... - Paxillin phosphorylation at Ser273 localizes a GIT1-PIX-PAK complex and regulates adhesion and protrusion dynamicsAnjana Nayal
Department of Cell Biology, University of Virginia, Charlottesville, VA 22908, USA
J Cell Biol 173:587-9. 2006..These adhesions appear to be regulated by the GIT1-PIX-PAK module near the leading edge... 
Cell migration: an overviewMiguel Vicente-Manzanares
Department of Cell Biology, University of Virginia School of Medicine, Charlottesville, VA, USA
Methods Mol Biol 769:1-24. 2011..The rest of the chapters are devoted to describe in detail some of the most salient technical advances that have illuminated the field of cell migration in recent years...- Segregation and activation of myosin IIB creates a rear in migrating cellsMiguel Vicente-Manzanares
Department of Cell Biology, University of Virginia, Charlottesville, VA 22908, USA
J Cell Biol 183:543-54. 2008.... - Myosin IIb activity and phosphorylation status determines dendritic spine and post-synaptic density morphologyJennifer L Hodges
Department of Cell Biology, University of Virginia School of Medicine, Charlottesville, Virginia, United States of America
PLoS ONE 6:e24149. 2011.... - Regulation of lamellipodial persistence, adhesion turnover, and motility in macrophages by focal adhesion kinaseKatherine A Owen
Department of Microbiology, University of Virginia Health System, Charlottesville, VA 22908, USA
J Cell Biol 179:1275-87. 2007..However, the combined loss of FAK and Pyk2 had no greater effect than the loss of either molecule alone, indicating that both kinases function within the same pathway to promote invasion... - Non-muscle myosin II takes centre stage in cell adhesion and migrationMiguel Vicente-Manzanares
Department of Cell Biology, University of Virginia School of Medicine, Charlottesville, Virginia 22908, USA
Nat Rev Mol Cell Biol 10:778-90. 2009.... - Integrins in cell migration--the actin connectionMiguel Vicente-Manzanares
Department of Cell Biology, University of Virginia School of Medicine, Charlottesville, VA 22908, USA
J Cell Sci 122:199-206. 2009..Regulation of the assembly and organization of adhesions and their linkage to actin relies on signaling pathways that converge on components that control actin polymerization and organization... - The integrin-ligand interaction regulates adhesion and migration through a molecular clutchLingfeng Chen
Department of Cell Biology, University of Virginia, Charlottesville, Virginia, United States of America
PLoS ONE 7:e40202. 2012.... 
Cell migration at a glanceMiguel Vicente-Manzanares
Department of Cell Biology, University of Virginia, Charlottesville, VA 22908, USA
J Cell Sci 118:4917-9. 2005- A role for the Rho-p160 Rho coiled-coil kinase axis in the chemokine stromal cell-derived factor-1alpha-induced lymphocyte actomyosin and microtubular organization and chemotaxisMiguel Vicente-Manzanares
Servicio de Inmunologia, Hospital de la Princesa, Universidad Autonoma de Madrid, C Diego de Leon, 62, E 28006 Madrid, Spain
J Immunol 168:400-10. 2002..Our results indicate that the Rho-p160ROCK axis plays a pivotal role in the control of the cell shape as a step before lymphocyte migration toward a chemotactic gradient... - The leukocyte cytoskeleton in cell migration and immune interactionsMiguel Vicente-Manzanares
, Hospital Universitario de la Princesa, , Spain
Int Rev Cytol 216:233-89. 2002..The impact of receptor-ligand binding on the functional responses and the rearrangement of the cytoskeleton is also examined... - The RhoA effector mDia is induced during T cell activation and regulates actin polymerization and cell migration in T lymphocytesMiguel Vicente-Manzanares
Servicio de Inmunologia, Hospital de la Princesa, Universidad Autonoma de Madrid, Madrid, Spain
J Immunol 171:1023-34. 2003..Together, our data demonstrate that RhoA is involved in the control of the filamentous actin/monomeric actin balance through mDia, and that this balance is critical for T cell responses... - Control of lymphocyte shape and the chemotactic response by the GTP exchange factor VavMiguel Vicente-Manzanares
Servicio de Inmunologia, Hospital Universitario de la Princesa, Madrid, Spain
Blood 105:3026-34. 2005..Together, our data postulate a pivotal role for Vav in the transmission of the migratory signal through the chemokine receptor CXCR4... - A novel serine-rich motif in the intercellular adhesion molecule 3 is critical for its ezrin/radixin/moesin-directed subcellular targetingJuan M Serrador
Servicio de Inmunologia, Hospital de la Princesa, Universidad Autonoma de Madrid, Madrid 28006, Spain
J Biol Chem 277:10400-9. 2002..Our results underscore the key role of specific serine residues within the cytoplasmic region of ICAM-3 for its ERM-directed positioning at the trailing edge of motile lymphocytes... - Dynamic interaction of VCAM-1 and ICAM-1 with moesin and ezrin in a novel endothelial docking structure for adherent leukocytesOlga Barreiro
Servicio de Inmunologia, Hospital de la Princesa, Universidad Autonoma de Madrid, 28006 Madrid, Spain
J Cell Biol 157:1233-45. 2002..These results provide the first characterization of an endothelial docking structure that plays a key role in the firm adhesion of leukocytes to the endothelium during inflammation... - A juxta-membrane amino acid sequence of P-selectin glycoprotein ligand-1 is involved in moesin binding and ezrin/radixin/moesin-directed targeting at the trailing edge of migrating lymphocytesJuan M Serrador
Servicio de Inmunologia, Hospital de la Princesa, Universidad Autonoma de Madrid, Madrid, Spain
Eur J Immunol 32:1560-6. 2002..These results underline the role of moesin in the subcellular redistribution of PSGL-1 in lymphoid cells and make evident the importance of specific serine residues within the cytoplasmic tail of PSGL-1 for this process... - Cell adhesion and polarity during immune interactionsMaria C Montoya
Servicio de Inmunologia, Hospital de la Princesa, Universidad Autonoma de Madrid, Madrid, Spain
Immunol Rev 186:68-82. 2002..The role of membrane microdomains, adaptor molecules and the cytoskeleton in the regulation of the molecular reorganization at cell-cell contacts is also discussed... - Cutting edge: association of the motor protein nonmuscle myosin heavy chain-IIA with the C terminus of the chemokine receptor CXCR4 in T lymphocytesMercedes Rey
Servicio de Inmunologia, Hospital de la Princesa, Universidad Autonoma de Madrid, C Diego de Leon 62, E 28006 Madrid, Spain
J Immunol 169:5410-4. 2002..These results provide the first evidence of a biochemical association between chemokine receptors and motor proteins, a mechanosignaling mechanism that may have a key role in lymphocyte migration... - Regulation of microtubule-organizing center orientation and actomyosin cytoskeleton rearrangement during immune interactionsDavid Sancho
, Hospital de la Princesa, , Madrid, Spain
Immunol Rev 189:84-97. 2002..We focus on the mechanisms that control the early MTOC reorientation during T cell-APC interaction and the relevance of this process to T cell activation... - Measurement of the levels of polymerized actin (F-actin) in chemokine-stimulated lymphocytes and GFP-coupled cDNA transfected lymphoid cells by flow cytometryMiguel Vicente-Manzanares
Immunology Service, Hospital Universitario de la Princesa, UAM, Madrid, Spain
Methods Mol Biol 239:53-68. 2004 - Signaling through the leukocyte integrin LFA-1 in T cells induces a transient activation of Rac-1 that is regulated by Vav and PI3K/Akt-1Lorena Sánchez-Martín
Instituto de Farmacologia y Toxicologia CSIC UCM, Facultad de Medicina UCM, Madrid 28040, Spain
J Biol Chem 279:16194-205. 2004.... - Lymphocyte chemotaxis is regulated by histone deacetylase 6, independently of its deacetylase activityJ Román Cabrero
Servicio de Inmunologia, Hospital de la Princesa, Universidad Autonoma de Madrid, 28006 Madrid, Spain
Mol Biol Cell 17:3435-45. 2006..Our results indicate that HDAC6 has an important role in the chemotaxis of T-lymphocytes, which is independent of its tubulin deacetylase activity... - Interactive protrusive structures during leukocyte adhesion and transendothelial migrationOlga Barreiro
Servicio de Inmunologia, Hospital de la Princesa, , 28006 Madrid, Spain
Front Biosci 9:1849-63. 2004..The adhesion receptor redistribution, cytoskeletal remodelling and intracellular signaling events that participate in this phenomenon are also discussed... - F-actin-dependent translocation of the Rap1 GDP/GTP exchange factor RasGRP2Mariía J Caloca
Centro de Investigacion del Cancer, University of Salamanca Consejo Superior de Investigaciones Cientiíficas, Campus Unamuno, E 37007 Salamanca, Spain
J Biol Chem 279:20435-46. 2004..These results reveal a novel function of the actin cytoskeleton in mediating the spatial activation of Ras subfamily GTPases through the selective recruitment of GDP/GTP exchange factors... - Role of the cytoskeleton during leukocyte responsesMiguel Vicente-Manzanares
, Hospital Universitario de la Princesa, , 28006-Madrid, Spain
Nat Rev Immunol 4:110-22. 2004..In this review, we discuss the composition and regulation of the different cytoskeletal elements and their role in immune responses... - Synaptic clusters of MHC class II molecules induced on DCs by adhesion molecule-mediated initial T-cell scanningHortensia de la Fuente
, Hospital de la Princesa, , 28006 Madrid, Spain
Mol Biol Cell 16:3314-22. 2005..Our results support a model in which ICAM-mediated MHC-II clustering on DC constitutes a priming mechanism to enhance antigen presentation to T-cells...