As Verkman

Summary

Affiliation: University of California
Country: USA

Publications

  1. ncbi Neuromyelitis optica pathology in rats following intraperitoneal injection of NMO-IgG and intracerebral needle injury
    Nithi Asavapanumas
    Departments of Medicine and Physiology, University of California, San Francisco, CA, USA
    Acta Neuropathol Commun 2:48. 2014
  2. pmc Convective washout reduces the antidiarrheal efficacy of enterocyte surface-targeted antisecretory drugs
    Byung Ju Jin
    Department of Medicine, University of California, San Francisco, San Francisco, CA 94143, USA
    J Gen Physiol 141:261-72. 2013
  3. pmc Fractionation of a herbal antidiarrheal medicine reveals eugenol as an inhibitor of Ca2+-Activated Cl- channel TMEM16A
    Zhen Yao
    Department of Medicine and Physiology, University of California San Francisco, San Francisco, California, United States of America
    PLoS ONE 7:e38030. 2012
  4. pmc Light inactivation of water transport and protein-protein interactions of aquaporin-Killer Red chimeras
    Florian Baumgart
    Department of Medicine, University of California, San Francisco, San Francisco, CA 94143, USA
    J Gen Physiol 139:83-91. 2012
  5. pmc Intravenous neuromyelitis optica autoantibody in mice targets aquaporin-4 in peripheral organs and area postrema
    Julien Ratelade
    Departments of Medicine and Physiology, University of California San Francisco, San Francisco, California, United States of America
    PLoS ONE 6:e27412. 2011
  6. ncbi Antidiarrheal efficacy and cellular mechanisms of a Thai herbal remedy
    Lukmanee Tradtrantip
    Departments of Medicine and Physiology, University of California, San Francisco, San Francisco, California, United States of America
    PLoS Negl Trop Dis 8:e2674. 2014
  7. pmc Airway surface liquid depth imaged by surface laser reflectance microscopy
    Jay R Thiagarajah
    Department of Medicine, University of California, San Francisco, CA 94143, USA
    J Gen Physiol 136:353-62. 2010
  8. pmc Aquaporin-4-dependent K(+) and water transport modeled in brain extracellular space following neuroexcitation
    Byung Ju Jin
    Department of Medicine, University of California, San Francisco, San Francisco, CA 94143, USA
    J Gen Physiol 141:119-32. 2013
  9. doi Aquaporin-1 gene deletion reduces breast tumor growth and lung metastasis in tumor-producing MMTV-PyVT mice
    Cristina Esteva-Font
    11246 Health Sciences East Tower, University of California, San Francisco, CA 94143 0521, USA
    FASEB J 28:1446-53. 2014
  10. ncbi Experimental mouse model of optic neuritis with inflammatory demyelination produced by passive transfer of neuromyelitis optica-immunoglobulin G
    Nithi Asavapanumas
    Department of Medicine and Physiology, University of California, 1246 Health Sciences East Tower, San Francisco, CA 94143 0521, USA
    J Neuroinflammation 11:16. 2014

Collaborators

Detail Information

Publications106 found, 100 shown here

  1. ncbi Neuromyelitis optica pathology in rats following intraperitoneal injection of NMO-IgG and intracerebral needle injury
    Nithi Asavapanumas
    Departments of Medicine and Physiology, University of California, San Francisco, CA, USA
    Acta Neuropathol Commun 2:48. 2014
    ..Animal models of neuromyelitis optica (NMO) are needed for drug testing and evaluation of NMO disease pathogenesis mechanisms...
  2. pmc Convective washout reduces the antidiarrheal efficacy of enterocyte surface-targeted antisecretory drugs
    Byung Ju Jin
    Department of Medicine, University of California, San Francisco, San Francisco, CA 94143, USA
    J Gen Physiol 141:261-72. 2013
    ..Efficacy of a surface-targeted permeable inhibitor delivered from the blood requires high inhibitor permeability and blood concentration (relative to K(d))...
  3. pmc Fractionation of a herbal antidiarrheal medicine reveals eugenol as an inhibitor of Ca2+-Activated Cl- channel TMEM16A
    Zhen Yao
    Department of Medicine and Physiology, University of California San Francisco, San Francisco, California, United States of America
    PLoS ONE 7:e38030. 2012
    ..TMEM16A Cl(-) channel inhibition adds to the list of eugenol molecular targets and may account for some of its biological activities...
  4. pmc Light inactivation of water transport and protein-protein interactions of aquaporin-Killer Red chimeras
    Florian Baumgart
    Department of Medicine, University of California, San Francisco, San Francisco, CA 94143, USA
    J Gen Physiol 139:83-91. 2012
    ..Our data also support the utility of CALI to study protein-protein interactions as well as other membrane transporters and receptors...
  5. pmc Intravenous neuromyelitis optica autoantibody in mice targets aquaporin-4 in peripheral organs and area postrema
    Julien Ratelade
    Departments of Medicine and Physiology, University of California San Francisco, San Francisco, California, United States of America
    PLoS ONE 6:e27412. 2011
    ..The rapid access of serum NMO-IgG to AQP4 in peripheral organs but not the CNS indicates that restricted antibody access cannot account for the absence of NMO pathology in peripheral organs...
  6. ncbi Antidiarrheal efficacy and cellular mechanisms of a Thai herbal remedy
    Lukmanee Tradtrantip
    Departments of Medicine and Physiology, University of California, San Francisco, San Francisco, California, United States of America
    PLoS Negl Trop Dis 8:e2674. 2014
    ....
  7. pmc Airway surface liquid depth imaged by surface laser reflectance microscopy
    Jay R Thiagarajah
    Department of Medicine, University of California, San Francisco, CA 94143, USA
    J Gen Physiol 136:353-62. 2010
    ..Compared with confocal imaging, ASL depth measurement by surface laser reflectance microscopy does not require dye staining or costly instrumentation, and can potentially be adapted for in vivo measurements using fiberoptics...
  8. pmc Aquaporin-4-dependent K(+) and water transport modeled in brain extracellular space following neuroexcitation
    Byung Ju Jin
    Department of Medicine, University of California, San Francisco, San Francisco, CA 94143, USA
    J Gen Physiol 141:119-32. 2013
    ....
  9. doi Aquaporin-1 gene deletion reduces breast tumor growth and lung metastasis in tumor-producing MMTV-PyVT mice
    Cristina Esteva-Font
    11246 Health Sciences East Tower, University of California, San Francisco, CA 94143 0521, USA
    FASEB J 28:1446-53. 2014
    ..005). These results implicate AQP1 as an important determinant of tumor angiogenesis and, hence, as a potential drug target for adjuvant therapy of solid tumors...
  10. ncbi Experimental mouse model of optic neuritis with inflammatory demyelination produced by passive transfer of neuromyelitis optica-immunoglobulin G
    Nithi Asavapanumas
    Department of Medicine and Physiology, University of California, 1246 Health Sciences East Tower, San Francisco, CA 94143 0521, USA
    J Neuroinflammation 11:16. 2014
    ..Most NMO patients are seropositive for immunoglobulin G autoantibodies (NMO-IgG) against the astrocyte water channel aquaporin-4 (AQP4)...
  11. doi Greatly improved survival and neuroprotection in aquaporin-4-knockout mice following global cerebral ischemia
    Ryuichi Katada
    1University of California, 1246 Health Sciences East Tower, Box 0521, San Francisco, CA 94143 0521, USA
    FASEB J 28:705-14. 2014
    ..AQP4 water transport inhibition may improve survival and neurological outcome after cardiac arrest and in other conditions associated with global cerebral ischemia...
  12. doi Microfluidics platform for single-shot dose-response analysis of chloride channel-modulating compounds
    Byung Ju Jin
    Departments of Medicine and Physiology, University of California, San Francisco, CA 94143, USA
    Lab Chip 13:3862-7. 2013
    ..The microfluidics approach developed here may accelerate the discovery and characterization of chloride channel-targeted drugs. ..
  13. pmc Therapeutic cleavage of anti-aquaporin-4 autoantibody in neuromyelitis optica by an IgG-selective proteinase
    Lukmanee Tradtrantip
    Departments of Medicine and Physiology, University of California, San Francisco, CA, USA
    Mol Pharmacol 83:1268-75. 2013
    ..IgG-selective cleavage by IdeS thus neutralizes NMO-IgG pathogenicity, and yields therapeutic F(ab')(2) and Fc fragments. IdeS treatment, by therapeutic apheresis or direct administration, may be beneficial in NMO...
  14. ncbi CFTR inhibitors
    Alan S Verkman
    University of California San Francisco, CA 94143 0521, U S A
    Curr Pharm Des 19:3529-41. 2013
    ..The most potent CFTR inhibitor has IC50 of approximately 4 nM. Studies in animal models support the development of CFTR inhibitors for antisecretory therapy of enterotoxin-mediated diarrheas and polycystic kidney disease...
  15. pmc Triazolothienopyrimidine inhibitors of urea transporter UT-B reduce urine concentration
    Chenjuan Yao
    Department of Medicine, University of California, San Francisco, USA
    J Am Soc Nephrol 23:1210-20. 2012
    ..The diuretic mechanism of UT inhibitors may complement the action of conventional diuretics, which target sodium transport...
  16. pmc Small-molecule inhibitors of NMO-IgG binding to aquaporin-4 reduce astrocyte cytotoxicity in neuromyelitis optica
    Lukmanee Tradtrantip
    Department of Medicine, University of California, San Francisco, CA 94143 0521, USA
    FASEB J 26:2197-208. 2012
    ..Small-molecule blockers may also be useful for other autoimmune diseases caused by binding of pathogenic autoantibodies to defined targets...
  17. pmc Greatly attenuated experimental autoimmune encephalomyelitis in aquaporin-4 knockout mice
    Lihua Li
    Department of Medicine, University of California, San Francisco, California, USA
    BMC Neurosci 10:94. 2009
    ....
  18. pmc Mammalian aquaporins: diverse physiological roles and potential clinical significance
    A S Verkman
    Department of Medicine, Cardiovascular Research Institute, 1246 Health Sciences East Tower, University of California, San Francisco, CA 94143 0521, USA
    Expert Rev Mol Med 10:e13. 2008
    ....
  19. pmc Aquaporins--new players in cancer biology
    A S Verkman
    Department of Medicine, University of California, 1246 Health Sciences East Tower, San Francisco, CA 94143 0521, USA
    J Mol Med (Berl) 86:523-9. 2008
    ..Together, the data suggest that AQP expression in tumor cells and tumor vessels facilitates tumor growth and spread, suggesting AQP inhibition as a novel antitumor therapy...
  20. pmc Aquaporin-4: orthogonal array assembly, CNS functions, and role in neuromyelitis optica
    Alan S Verkman
    Department of Medicine, University of California, San Francisco, CA 94143 0521, USA
    Acta Pharmacol Sin 32:702-10. 2011
    ....
  21. pmc Aquaporins: translating bench research to human disease
    A S Verkman
    Department of Medicine, Cardiovascular Research Institute, University of California, San Francisco, CA 94143, USA
    J Exp Biol 212:1707-15. 2009
    ..These exciting possibilities and their associated challenges are reviewed...
  22. pmc Functions of aquaporins in the eye
    A S Verkman
    Department of Medicine and Physiology, University of California, San Francisco, 1246 Health Sciences East Tower, San Francisco, CA 94143 0521, USA
    Prog Retin Eye Res 27:420-33. 2008
    ..However, much research remains in defining cell-level mechanisms for the ocular AQP functions, in establishing the relevance to human eye disease of conclusions from knockout mice, and in developing AQP-modulating drugs...
  23. pmc Dissecting the roles of aquaporins in renal pathophysiology using transgenic mice
    A S Verkman
    Department of Medicine, Cardiovascular Research Institute, University of California, San Francisco, CA 94143 0521, USA
    Semin Nephrol 28:217-26. 2008
    ..Future work in renal AQPs will focus on mechanisms responsible for these non-fluid-transporting functions, and on the development of small-molecule AQP inhibitors for use as aquaretic-type diuretics...
  24. pmc Chloride channels as drug targets
    Alan S Verkman
    Departments of Medicine and Physiology, University of California, San Francisco, California 94143 0521, USA
    Nat Rev Drug Discov 8:153-71. 2009
    ..Here, we discuss the broad opportunities that remain in chloride-channel-based drug discovery...
  25. pmc Aquaporins in clinical medicine
    A S Verkman
    Department of Medicine, University of California, San Francisco, California 94143 0521, USA
    Annu Rev Med 63:303-16. 2012
    ..There is great promise in the development of small-molecule aquaporin modulators for therapy of some types of refractory edema, brain swelling, neuroinflammation, glaucoma, epilepsy, cancer, pain, and obesity...
  26. pmc Evidence against aquaporin-1-dependent CO2 permeability in lung and kidney
    Xiaohui Fang
    Departments of Medicine and Physiology, Cardiovascular Research Institute, University of California, San Francisco, CA 94143 0521, USA
    J Physiol 542:63-9. 2002
    ..The results provide direct evidence against physiologically significant transport of CO2 by AQP1 in mammalian lung and kidney...
  27. ncbi Erythrocyte water permeability and renal function in double knockout mice lacking aquaporin-1 and aquaporin-3
    B Yang
    Department of Medicine, Cardiovascular Research Institute, University of California, San Francisco, California, 94143 0521, USA
    J Biol Chem 276:624-8. 2001
    ..The renal function studies indicate independent roles of AQP1 and AQP3 in countercurrent exchange and collecting duct osmotic equilibration, respectively...
  28. ncbi Evidence against a role of mouse, rat, and two cloned human t1alpha isoforms as a water channel or a regulator of aquaporin-type water channels
    T Ma
    Departments of Medicine and Physiology, Cardiovascular Research Institute, University of California, San Francisco, California, USA
    Am J Respir Cell Mol Biol 19:143-9. 1998
    ..The cloning of hT1alpha isoforms may permit the development of an assay of type I cell antigen in airspace fluid as a marker of human lung injury...
  29. ncbi Diffusional mobility of the cystic fibrosis transmembrane conductance regulator mutant, delta F508-CFTR, in the endoplasmic reticulum measured by photobleaching of GFP-CFTR chimeras
    Peter M Haggie
    Department of Medicine, Cardiovascular Research Institute, University of California, San Francisco, California 94143 0521, USA
    J Biol Chem 277:16419-25. 2002
    ....
  30. ncbi Pleural surface fluorescence measurement of Na+ and Cl- transport across the air space-capillary barrier
    Jinjun Jiang
    Department of Medicine, Cardiovascular Research Institute, University of California, San Francisco, 94143 0521, USA
    J Appl Physiol 94:343-52. 2003
    ..Our results establish a pleural surface fluorescence method to measure unidirectional Cl(-) and Na(+) flux in intact lung and provide evidence for cAMP-stimulated transcellular Cl(-) and Na(+) transport...
  31. ncbi A small molecule CFTR inhibitor produces cystic fibrosis-like submucosal gland fluid secretions in normal airways
    Jay R Thiagarajah
    Department of Medicine, Cardiovascular Research Institute, University of California, San Francisco, California, USA
    FASEB J 18:875-7. 2004
    ....
  32. ncbi Increased diffusional mobility of CFTR at the plasma membrane after deletion of its C-terminal PDZ binding motif
    Peter M Haggie
    Departments of Medicine and Physiology, Cardiovascular Research Institute, University of California, San Francisco, California 94143 0521, USA
    J Biol Chem 279:5494-500. 2004
    ..PDZ-domain interactions of CFTR are thus dynamic and occur on a time scale of seconds or faster...
  33. pmc Thiophenecarboxylate suppressor of cyclic nucleotides discovered in a small-molecule screen blocks toxin-induced intestinal fluid secretion
    Lukmanee Tradtrantip
    Department of Medicine, Cardiovascular Research Institute, University of California, San Francisco, CA 94143 0521, USA
    Mol Pharmacol 75:134-42. 2009
    ....
  34. ncbi cDNA and genomic cloning of mouse aquaporin-2: functional analysis of an orthologous mutant causing nephrogenic diabetes insipidus
    B Yang
    Department of Physiology, Cardiovascular Research Institute, San Francisco, California, 94143 0521, USA
    Genomics 57:79-83. 1999
    ..The chemical chaperone glycerol produced a redistribution of Aqp2-T126M from ER to plasma membrane/endosomes. These results establish a basis for an Aqp2-T126M transgenic knock-in model of NDI...
  35. ncbi The mercurial insensitive water channel (AQP-4) forms orthogonal arrays in stably transfected Chinese hamster ovary cells
    B Yang
    Department of Medicine, Cardiovascular Research Institute, University of California, San Francisco, California 94143 0521, USA
    J Biol Chem 271:4577-80. 1996
    ..These results provide direct evidence that a molecular water channel can spontaneously assemble in regular arrays...
  36. ncbi Nanomolar affinity small molecule correctors of defective Delta F508-CFTR chloride channel gating
    Hong Yang
    Department of Medicine, University of California, San Francisco, California 94143, USA
    J Biol Chem 278:35079-85. 2003
    ..In conjunction with correctors of defective Delta F508-CFTR processing, small molecule potentiators of defective Delta F508-CFTR gating may be useful for therapy of cystic fibrosis caused by the Delta F508 mutation...
  37. ncbi Phenotype analysis of aquaporin-8 null mice
    Baoxue Yang
    Cardiovascular Research Institute, Univ of California, San Francisco, CA 94143 0521, USA
    Am J Physiol Cell Physiol 288:C1161-70. 2005
    ..The mild phenotype of AQP8-null mice was surprising in view of the multiple phenotype abnormalities found in mouse models of AQP1-5 deficiency...
  38. ncbi ClC-3 chloride channels facilitate endosomal acidification and chloride accumulation
    Mariko Hara-Chikuma
    Departments of Medicine and Physiology, Cardiovascular Research Institute, University of California, San Francisco, California 94143 0521, USA
    J Biol Chem 280:1241-7. 2005
    ..3 versus 5.9. Our results provide direct evidence for involvement of ClC-3 in endosomal acidification by Cl- shunting of the interior-positive membrane potential created by the vacuolar H+ pump...
  39. ncbi Evidence against functionally significant aquaporin expression in mitochondria
    Baoxue Yang
    Departments of Medicine and Physiology, Cardiovascular Research Institute, University of California San Francisco, San Francisco, CA 94143 0521, USA
    J Biol Chem 281:16202-6. 2006
    ....
  40. ncbi High-affinity activators of cystic fibrosis transmembrane conductance regulator (CFTR) chloride conductance identified by high-throughput screening
    Tonghui Ma
    Department of Medicine, Cardiovascular Research Institute, University of California, San Francisco, California 94143 0521, USA
    J Biol Chem 277:37235-41. 2002
    ..The new activators identified here may be useful in defining molecular mechanisms of CFTR activation and as lead compounds in CF drug development...
  41. ncbi Role of aquaporin water channels in pleural fluid dynamics
    Y Song
    Departments of Medicine and Physiology, Cardiovascular Research Institute, University of California, San Francisco, California 94143 0521, USA
    Am J Physiol Cell Physiol 279:C1744-50. 2000
    ..However, AQP1 does not appear to play a role in clinically relevant mechanisms of pleural fluid accumulation or clearance...
  42. pmc In situ fluorescence measurement of tear film [Na+], [K+], [Cl-], and pH in mice shows marked hypertonicity in aquaporin-5 deficiency
    Javier Ruiz-Ederra
    Department of Medicine and Physiology, Cardiovascular Research Institute, University of California, San Francisco, California 94143 0521, USA
    Invest Ophthalmol Vis Sci 50:2132-8. 2009
    ....
  43. ncbi Aquaporin-dependent water permeation at the mouse ocular surface: in vivo microfluorimetric measurements in cornea and conjunctiva
    Marc H Levin
    Department of Medicine and Physiology, Cardiovascular Research Institute, University of California San Francisco, San Francisco, CA 94143 0521, USA
    Invest Ophthalmol Vis Sci 45:4423-32. 2004
    ....
  44. pmc Role of aquaporin water channels in airway fluid transport, humidification, and surface liquid hydration
    Y Song
    Department of Medicine, Cardiovascular Research Institute, University of California, San Francisco, California 94143, USA
    J Gen Physiol 117:573-82. 2001
    ..These results establish the fluid transporting properties of mouse airways, and indicate that aquaporins play at most a minor role in airway humidification, ASL hydration, and isosmolar fluid absorption...
  45. ncbi Tear secretion by lacrimal glands in transgenic mice lacking water channels AQP1, AQP3, AQP4 and AQP5
    M Moore
    Department of Medicine, University of California, San Francisco 94143 0521, USA
    Exp Eye Res 70:557-62. 2000
    ..The requirement for aquaporins in salivary but not lacrimal gland secretion, may involve the substantially slower fluid secretion rate across lacrimal gland acinar cells...
  46. pmc Role of aquaporins in alveolar fluid clearance in neonatal and adult lung, and in oedema formation following acute lung injury: studies in transgenic aquaporin null mice
    Y Song
    Departments of Medicine and Physiology, Cardiovascular Research Institute, University of California, San Francisco, San Francisco, CA 94143, USA
    J Physiol 525:771-9. 2000
    ....
  47. ncbi Impairment of angiogenesis and cell migration by targeted aquaporin-1 gene disruption
    Samira Saadoun
    Department of Medicine, Cardiovascular Research Institute, University of California, San Francisco, San Francisco, California 94143, USA
    Nature 434:786-92. 2005
    ..Our findings support a fundamental role of water channels in cell migration, which is central to diverse biological phenomena including angiogenesis, wound healing, tumour spread and organ regeneration...
  48. pmc Glial cell aquaporin-4 overexpression in transgenic mice accelerates cytotoxic brain swelling
    Baoxue Yang
    Departments of Medicine and Physiology, University of California San Francisco, 1246 Health Sciences East Tower, San Francisco, CA 94143 0521, USA
    J Biol Chem 283:15280-6. 2008
    ..We conclude that AQP4 expression is rate-limiting for brain water accumulation, and thus, that altered AQP4 expression can be functionally significant...
  49. ncbi Cloning of a novel rat kidney cDNA homologous to CHIP28 and WCH-CD water channels
    T Ma
    Department of Medicine, University of California, San Francisco 94143 0532
    Biochem Biophys Res Commun 197:654-9. 1993
    ..WCH3 is a new member of the MIP26 family of channel-forming proteins in mammalian kidney...
  50. ncbi Optical measurement of swelling and water transport in spinal cord slices from aquaporin null mice
    E I Solenov
    Departments of Medicine and Physiology, 1246 Health Sciences East Tower, Cardiovascular Research Institute, University of California, San Francisco, CA 94143 0521, USA
    J Neurosci Methods 113:85-90. 2002
    ..These results establish a simple imaging method to map changes in water content of spinal cord slices, and provide evidence that aquaporins facilitate osmotic water transport in functionally relevant areas of the spinal cord...
  51. ncbi Airway surface liquid pH in well-differentiated airway epithelial cell cultures and mouse trachea
    S Jayaraman
    Department of Medicine, Cardiovascular Research Institute, University of California, San Francisco, California 94143-0521, USA
    Am J Physiol Cell Physiol 281:C1504-11. 2001
    ..The rapid response of ASL pH to changes in systemic acid-base status may contribute to airway hypersensitivity in asthma and other airway diseases...
  52. ncbi cDNA cloning and gene structure of a novel water channel expressed exclusively in human kidney: evidence for a gene cluster of aquaporins at chromosome locus 12q13
    T Ma
    Department of Medicine, University of California, San Francisco 94143 0521, USA
    Genomics 35:543-50. 1996
    ..Further work is needed to establish the physiological significance of hKID...
  53. ncbi Severely impaired urinary concentrating ability in transgenic mice lacking aquaporin-1 water channels
    T Ma
    Department of Medicine, Cardiovascular Research Institute, University of California, San Francisco, California 94143 0521, USA
    J Biol Chem 273:4296-9. 1998
    ..The results suggest that AQP1 knockout mice are unable to create a hypertonic medullary interstitium by countercurrent multiplication. AQP1 is thus required for the formation of a concentrated urine by the kidney...
  54. pmc Colon water transport in transgenic mice lacking aquaporin-4 water channels
    K S Wang
    Department of Surgery, Stanford University School of Medicine, CA 94143 0521, USA
    Am J Physiol Gastrointest Liver Physiol 279:G463-70. 2000
    ....
  55. ncbi Molecular cloning of a mercurial-insensitive water channel expressed in selected water-transporting tissues
    H Hasegawa
    Department of Medicine and Physiology, University of California, San Francisco 94143 0532
    J Biol Chem 269:5497-500. 1994
    ..The MIWC water channel may participate in the urinary concentrating mechanism, the absorption of cerebrospinal fluid, and other physiological processes...
  56. ncbi In vivo measurement of brain extracellular space diffusion by cortical surface photobleaching
    Devin K Binder
    Department of Medicine, Cardiovascular Research Institute, University of California, San Francisco, San Francisco, California 94143 0521, USA
    J Neurosci 24:8049-56. 2004
    ....
  57. ncbi Expression, functional analysis, and in situ hybridization of a cloned rat kidney collecting duct water channel
    T Ma
    Department of Medicine, University of California, San Francisco 94143
    Am J Physiol 266:C189-97. 1994
    ..abstract truncated at 250 words)..
  58. ncbi Chloride accumulation and swelling in endosomes enhances DNA transfer by polyamine-DNA polyplexes
    N D Sonawane
    Departments of Medicine and Physiology, Cardiovascular Research Institute, University of California, San Francisco, California 94143, USA
    J Biol Chem 278:44826-31. 2003
    ..Our results provide direct support for the proton sponge hypothesis and thus a rational basis for the design of improved non-viral vectors for gene delivery...
  59. pmc Lung fluid transport in aquaporin-5 knockout mice
    T Ma
    Department of Medicine, Cardiovascular Research Institute, University of California, San Francisco, California 94143, USA
    J Clin Invest 105:93-100. 2000
    ..The unimpaired alveolar fluid clearance in AQP5-null mice indicates that high alveolar water permeability is not required for active, near-isosmolar fluid transport...
  60. ncbi Aquaporin deletion in mice reduces corneal water permeability and delays restoration of transparency after swelling
    Jay R Thiagarajah
    Department of Medicine, Cardiovascular Research Institute, University of California, San Francisco, California 94143 0521, USA
    J Biol Chem 277:19139-44. 2002
    ..The up-regulation of AQP1 expression and/or function in corneal endothelium may reduce corneal swelling and opacification following injury...
  61. ncbi Aquaporin-3-dependent cell migration and proliferation during corneal re-epithelialization
    Marc H Levin
    Department of Medicine and Physiology, Cardiovascular Research Institute, Graduate Group in Biophysics, University of California, San Francisco, California 94143 0521, USA
    Invest Ophthalmol Vis Sci 47:4365-72. 2006
    ..To determine a role for the water- and glycerol-transporting protein aquaporin-3 (AQP3) in mammalian corneal epithelium, where it is expressed but has no known function...
  62. pmc Airway surface liquid osmolality measured using fluorophore-encapsulated liposomes
    S Jayaraman
    Department of Medicine, Cardiovascular Research Institute, University of California, San Francisco, San Francisco, California, 94143, USA
    J Gen Physiol 117:423-30. 2001
    ..ASL fluid is approximately isosmolar under normal physiological conditions, but can become hyperosmolar when exposed to dry air, which may induce cough and airway reactivity in some patients...
  63. ncbi Diffusion in the endoplasmic reticulum of an aquaporin-2 mutant causing human nephrogenic diabetes insipidus
    M H Levin
    Departments of Medicine and Physiology, Cardiovascular Research Institute, University of California, San Francisco, California 94143 0521
    J Biol Chem 276:21331-6. 2001
    ..These results indicate that the ER retention of AQP2-T126M does not result from restricted or slowed mobility and suggest that the majority of AQP2-T126M is not aggregated or bound to slowly moving membrane proteins...
  64. pmc In situ measurement of airway surface liquid [K+] using a ratioable K+-sensitive fluorescent dye
    Wan Namkung
    Departments of Medicine and Physiology, University of California, San Francisco, California 94143, USA
    J Biol Chem 284:15916-26. 2009
    ..Our results provide the first noninvasive measurements of [K(+)] in the ASL and indicate the involvement of apical and basolateral membrane ion transporters in maintaining a high ASL [K(+)]...
  65. ncbi Gene structure, cDNA cloning, and expression of a mouse mercurial-insensitive water channel
    T Ma
    Department of Medicine, University of California, San Francisco, California, 94143 0521, USA
    Genomics 33:382-8. 1996
    ..The genomic cloning of mMIWC represents the first step in the construction of a targeting vector for mMIWC gene knockout...
  66. ncbi Aquaporins and CFTR in ocular epithelial fluid transport
    M H Levin
    Department of Medicine, Cardiovascular Research Institute, Graduate Group in Biophysics, University of California, San Francisco, CA 94143 0521, USA
    J Membr Biol 210:105-15. 2006
    ..Recent data suggest AQPs and CFTR as attractive targets for drug development for therapy of keratoconjunctivitis sicca, recurrent corneal erosions, corneal edema, glaucoma, retinal detachment, and retinal ischemia...
  67. ncbi Aquaporin-4 facilitates reabsorption of excess fluid in vasogenic brain edema
    Marios C Papadopoulos
    Department of Medicine, Cardiovascular Research Institute, University of California, San Francisco, CA, 94143 0521, USA
    FASEB J 18:1291-3. 2004
    ..Thus, AQP4-mediated transcellular water movement is crucial for fluid clearance in vasogenic brain edema, suggesting AQP4 activation and/or up-regulation as a novel therapeutic option in vasogenic brain edema...
  68. pmc Cloning, functional analysis and cell localization of a kidney proximal tubule water transporter homologous to CHIP28
    R Zhang
    Department of Medicine, University of California, San Francisco 94143 0532
    J Cell Biol 120:359-69. 1993
    ..These results indicate that CHIP28k is a major and highly selective water transporting protein in the kidney proximal tubule and thin descending limb of Henle, but not collecting duct...
  69. ncbi Sevenfold-reduced osmotic water permeability in primary astrocyte cultures from AQP-4-deficient mice, measured by a fluorescence quenching method
    Eugen Solenov
    Department of Medicine and Physiology, Cardiovascular Research Institute, University of California, San Francisco, San Francisco, CA 94143 0521, USA
    Am J Physiol Cell Physiol 286:C426-32. 2004
    ..3 +/- 0.5 vs. 5.5 +/- 0.4 kcal/mol). Our studies establish a calcein quenching method for measurement of cell membrane water permeability and indicate that AQP-4 provides the principal route for water transport in astrocytes...
  70. pmc Small-molecule correctors of defective DeltaF508-CFTR cellular processing identified by high-throughput screening
    Nicoletta Pedemonte
    Department of Medicine, UCSF, San Francisco, California 94143 0521, USA
    J Clin Invest 115:2564-71. 2005
    ..Small-molecule correctors may be useful in the treatment of CF caused by the DeltaF508 mutation...
  71. ncbi Reduced osmotic water permeability of the peritoneal barrier in aquaporin-1 knockout mice
    B Yang
    Departments of Medicine and Physiology, Cardiovascular Research Institute, University of California, San Francisco, California 94143 0521, USA
    Am J Physiol 276:C76-81. 1999
    ..05 and 0.46 +/- 0.04 ml/h in (+/+) and (-/-) mice, respectively] were not affected by AQP1 deletion. Therefore, AQP1 provides a major route for osmotically driven water transport across the peritoneal barrier in peritoneal dialysis...
  72. pmc Lung fluid transport in aquaporin-1 and aquaporin-4 knockout mice
    C Bai
    Departments of Medicine and Physiology, Cardiovascular Research Institute, University of California San Francisco, San Francisco, California 94143 0521, USA
    J Clin Invest 103:555-61. 1999
    ..AQP1 facilitates hydrostatically driven lung edema but is not required for active near-isosmolar absorption of alveolar fluid...
  73. ncbi cDNA cloning, gene organization, and chromosomal localization of a human mercurial insensitive water channel. Evidence for distinct transcriptional units
    B Yang
    Department of Medicine, University of California, San Francisco 94143 0521, USA
    J Biol Chem 270:22907-13. 1995
    ..The structural organization of the hMIWC gene represents a first step in definition of hMIWC differential expression, regulation, and possible role in human disease...
  74. pmc Aquaporin water channels in transepithelial fluid transport
    Lukmanee Tradtrantip
    Department of Medicine, University of California, San Francisco, CA 94143 0521, USA
    J Med Invest 56:179-84. 2009
    ....
  75. pmc CFTR-adenylyl cyclase I association responsible for UTP activation of CFTR in well-differentiated primary human bronchial cell cultures
    Wan Namkung
    Department of Medicine and Physiology, University of California, San Francisco, CA 94143, USA
    Mol Biol Cell 21:2639-48. 2010
    ..We further conclude that CFTR is the principal chloride secretory pathway in non-CF airways for both cAMP and Ca(2+) agonists, providing a novel mechanism to link CFTR dysfunction to CF lung disease...
  76. pmc Nephrogenic diabetes insipidus in mice lacking aquaporin-3 water channels
    T Ma
    Departments of Medicine, Physiology, and Pediatrics, Cardiovascular Research Institute, University of California, San Francisco, CA 94143 0521, USA
    Proc Natl Acad Sci U S A 97:4386-91. 2000
    ..Basolateral membrane aquaporins may thus provide blood-accessible targets for drug discovery of aquaretic inhibitors...
  77. ncbi Carbon dioxide permeability of aquaporin-1 measured in erythrocytes and lung of aquaporin-1 null mice and in reconstituted proteoliposomes
    B Yang
    Department of Medicine, Cardiovascular Research Institute, University of California, San Francisco, California 94143 0521, USA
    J Biol Chem 275:2686-92. 2000
    ..These results provide direct evidence against physiologically significant AQP1-mediated CO(2) transport and establish an upper limit to the CO(2) permeability through single AQP1 water channels...
  78. pmc Generation and phenotype of a transgenic knockout mouse lacking the mercurial-insensitive water channel aquaporin-4
    T Ma
    Department of Medicine, Cardiovascular Research Institute, University of California, San Francisco, California 94143 0521, USA
    J Clin Invest 100:957-62. 1997
    ....
  79. pmc Role of aquaporin-4 in airspace-to-capillary water permeability in intact mouse lung measured by a novel gravimetric method
    Y Song
    Department of Medicine, Cardiovascular Research Institute, University of California, San Francisco, San Francisco, California 94143, USA
    J Gen Physiol 115:17-27. 2000
    ..AQP1 null mice. These results establish a simple gravimetric method to quantify osmosis and filtration in intact mouse lung and provide direct evidence for a contribution of the distal airways to airspace-to-capillary water transport...
  80. ncbi Accelerated cataract formation and reduced lens epithelial water permeability in aquaporin-1-deficient mice
    Javier Ruiz-Ederra
    Department of Medicine, Cardiovascular Research Institute, University of California, San Francisco, California 94143 0521, USA
    Invest Ophthalmol Vis Sci 47:3960-7. 2006
    ..To investigate the involvement of aquaporin (AQP)-1 in lens epithelial cell water permeability and maintenance of lens transparency in experimental models of cataract formation...
  81. ncbi Submucosal gland dysfunction as a primary defect in cystic fibrosis
    Danieli Salinas
    Department of Medicine, University of California, San Francisco, California 94143 0521, USA
    FASEB J 19:431-3. 2005
    ..Our results suggest that defective airway submucosal gland function is an early, primary defect in CF. Therapies directed at normalizing gland fluid secretion early in CF may thus reduce lung disease...
  82. ncbi Urearetics: a small molecule screen yields nanomolar potency inhibitors of urea transporter UT-B
    Marc H Levin
    Department of Medicine, Cardiovascular Research Institute, University of California, San Francisco, San Francisco, CA 94143 0521, USA
    FASEB J 21:551-63. 2007
    ..UT-B inhibitors represent a new class of diuretics, "urearetics," which are predicted to increase renal water and solute clearance in water-retaining states...
  83. ncbi Hypertonic saline therapy in cystic fibrosis: Evidence against the proposed mechanism involving aquaporins
    Marc H Levin
    Department of Medicine, University of California, San Francisco, California 94143 0521, USA
    J Biol Chem 281:25803-12. 2006
    ..Our data provide evidence against the mechanism proposed by Donaldson et al. to account for the effects of amiloride and hypertonic saline in CF lung disease, indicating the need to identify alternate mechanisms...
  84. ncbi Increased seizure threshold in mice lacking aquaporin-4 water channels
    Devin K Binder
    Department of Neurological Surgery, University of California, 505 Parnassus Ave, Box 0112, San Francisco, CA 94143, USA
    Neuroreport 15:259-62. 2004
    ..These results suggest that glial water channels may modulate brain excitability and the initiation and generalization of seizure activity...
  85. ncbi Cell-based assay for high-throughput quantitative screening of CFTR chloride transport agonists
    L V Galietta
    Department of Medicine and Physiology, Cardiovascular Research Institute, University of California, San Francisco, California 94143-0521, USA
    Am J Physiol Cell Physiol 281:C1734-42. 2001
    ..The fluorescence assay and cell lines should facilitate the screening of novel CFTR activators and the characterization of alternative Cl(-) channels and transporters...
  86. ncbi Small-molecule screen identifies inhibitors of a human intestinal calcium-activated chloride channel
    Ricardo de la Fuente
    1246 Health Sciences East Tower, Box 0521, University of California, San Francisco CA 94143 0521, USA
    Mol Pharmacol 73:758-68. 2008
    ..Small-molecule CaCC inhibitors may be useful in pharmacological dissection of CaCC functions and in reducing intestinal fluid losses in CaCC-mediated secretory diarrheas...
  87. pmc Extracellular space diffusion in central nervous system: anisotropic diffusion measured by elliptical surface photobleaching
    Marios C Papadopoulos
    Departments of Medicine and Physiology, Cardiovascular Research Institute, 1246 Health Sciences East Tower, University of California San Francisco, San Francisco, CA 94143, USA
    Biophys J 89:3660-8. 2005
    ..We suggest that the extracellular matrix might have evolved to facilitate rather than hinder diffusion even for large molecules...
  88. pmc Small-molecule activators of TMEM16A, a calcium-activated chloride channel, stimulate epithelial chloride secretion and intestinal contraction
    Wan Namkung
    Department of Medicine and Physiology, University of California, San Francisco, California 94143 0521, USA
    FASEB J 25:4048-62. 2011
    ..Small-molecule, TMEM16A-targeted activators may be useful for drug therapy of cystic fibrosis, dry mouth, and gastrointestinal hypomotility disorders, and for pharmacological dissection of TMEM16A function...
  89. pmc Cyanoquinolines with independent corrector and potentiator activities restore ΔPhe508-cystic fibrosis transmembrane conductance regulator chloride channel function in cystic fibrosis
    Puay Wah Phuan
    Department of Medicine, University of California, San Francisco, California, USA
    Mol Pharmacol 80:683-93. 2011
    ..Compounds with dual corrector and potentiator activities may be useful for single-drug treatment of cystic fibrosis caused by ΔPhe508 mutation...
  90. pmc Hyperviscous airway periciliary and mucous liquid layers in cystic fibrosis measured by confocal fluorescence photobleaching
    Nico Derichs
    Department of Medicine, University of California, San Francisco, California, USA
    FASEB J 25:2325-32. 2011
    ..Our results challenge the view that the PCL is a relatively watery, nonviscous fluid layer in contact with a more viscous ML, and offer an explanation for CF lung disease in the gland-free lower airways...
  91. pmc Proinflammatory role of aquaporin-4 in autoimmune neuroinflammation
    Lihua Li
    Department of Medicine, University of California, San Francisco, California 94143 0521, USA
    FASEB J 25:1556-66. 2011
    ..Our data suggest an intrinsic proinflammatory role of AQP4 involving AQP4-dependent astrocyte swelling and cytokine release. Reduction in AQP4 water transport may be protective in neuroinflammatory CNS diseases...
  92. pmc Crofelemer, an antisecretory antidiarrheal proanthocyanidin oligomer extracted from Croton lechleri, targets two distinct intestinal chloride channels
    Lukmanee Tradtrantip
    Department of Medicine, University of California, San Francisco, San Francisco, CA 94143 0521, USA
    Mol Pharmacol 77:69-78. 2010
    ..5 microM. The dual inhibitory action of crofelemer on two structurally unrelated prosecretory intestinal Cl(-) channels may account for its intestinal antisecretory activity...
  93. ncbi Role of RhAG and AQP1 in NH3 and CO2 gas transport in red cell ghosts: a stopped-flow analysis
    P Ripoche
    Institut National de la Transfusion Sanguine, Paris F 75015, France
    Transfus Clin Biol 13:117-22. 2006
    ....
  94. ncbi Cardiac aquaporin expression in humans, rats, and mice
    Tanya L Butler
    Kids Heart Research, Children s Hospital at Westmead, Locked Bag 4001, Westmead, NSW 2145, Australia
    Am J Physiol Heart Circ Physiol 291:H705-13. 2006
    ..These studies support a possible functional role of AQP-1 in myocardium but indicate that early adaptations to osmotic and ischemic stress do not involve transcriptional or posttranslational AQP-1 regulation...
  95. ncbi Aquaporin-4 gene disruption in mice reduces brain swelling and mortality in pneumococcal meningitis
    Marios C Papadopoulos
    Department of Medicine, Cardiovascular Research Institute, University of California, San Francisco, California 94143 0521, USA
    J Biol Chem 280:13906-12. 2005
    ..Our findings provide a novel molecular mechanism for the pathogenesis of brain edema in acute bacterial meningitis, and suggest that inhibition of AQP4 function or up-regulation may dramatically improve clinical outcome...
  96. ncbi Absence of orthogonal arrays in kidney, brain and muscle from transgenic knockout mice lacking water channel aquaporin-4
    J M Verbavatz
    Service de Biologie Cellulaire, CEA Saclay, Gif sur Yvette, France
    J Cell Sci 110:2855-60. 1997
    ..These studies provide direct evidence that AQP4 is required for formation of OAPs and is a component of OAPs, thus establishing the identity and function of OAPs...
  97. ncbi Neonatal mortality in an aquaporin-2 knock-in mouse model of recessive nephrogenic diabetes insipidus
    B Yang
    Departments of Medicine, Physiology, and Pediatrics, Cardiovascular Research Institute, University of California, San Francisco 94143 0521, USA
    J Biol Chem 276:2775-9. 2001
    ..Our results establish a mouse model of human autosomal NDI and provide the first in vivo biochemical data on a disease-causing AQP2 mutant...
  98. ncbi Fourfold reduction of water permeability in inner medullary collecting duct of aquaporin-4 knockout mice
    C L Chou
    Laboratory of Kidney and Electrolyte Metabolism, National Institutes of Health, Bethesda, Maryland 20892 1603, USA
    Am J Physiol 274:C549-54. 1998
    ..These results indicate that AQP4 is responsible for the majority of basolateral membrane water movement in IMCD but that its deletion is associated with a very mild defect in urinary concentrating ability...
  99. ncbi Aquaporin-1 plays an essential role in water permeability and ultrafiltration during peritoneal dialysis
    J Ni
    Division of Nephrology and Department of Pathology, UCL Medical School, Brussels, Belgium
    Kidney Int 69:1518-25. 2006
    ..They validate essential predictions of the three-pore model: (i) the ultrasmall pores account for the sodium sieving, and (ii) they mediate 50% of UF during a hypertonic dwell...
  100. ncbi Small-molecule vasopressin-2 receptor antagonist identified by a g-protein coupled receptor "pathway" screen
    Buranee Yangthara
    Department of Medicine, Cardiovascular Research Institute, University of California, San Francisco, California 94143 0521, USA
    Mol Pharmacol 72:86-94. 2007
    ..The CFTR-linked cAMP assay developed here is applicable for efficient, high-throughput identification of modulators of cAMP-coupled GPCRs...
  101. ncbi Defective water and glycerol transport in the proximal tubules of AQP7 knockout mice
    Eisei Sohara
    Department of Nephrology, Graduate School of Medicine, Tokyo Medical and Dental University, Japan
    Am J Physiol Renal Physiol 289:F1195-200. 2005
    ..007 +/- 0.005 mg/ml; cisplatin, 0.063 +/- 0.043 mg/ml; ischemia, 0.076 +/- 0.02 mg/ml), suggesting that urine glycerol could be used as a new biomarker for detecting proximal straight tubule injury...

Research Grants47

  1. Functional Role of Aquaporins in Eye Physiology
    Alan S Verkman; Fiscal Year: 2010
    ..Together, our studies will establish new roles of aquaporins in the eye with direct implications for new therapies. ..
  2. Role of ASL and Airway Glands in CF Lung Disease
    Alan Verkman; Fiscal Year: 2006
    ..Understanding of lung disease mechanisms is of central importance in CF research, having direct implications for development of evaluation of new therapies. ..
  3. Functional Role of Aquaporins in Eye Physiology
    Alan Verkman; Fiscal Year: 2006
    ..If aquaporins are proven to be important, as anticipated, then modulation of aquaporin expression and/or function might provide a new therapeutic strategy for treatment of glaucoma and corneal edema. ..
  4. PHYSIOLOGY AND MOLECULAR BIOLOGY OF LUNG WATER TRANSPORT
    Alan Verkman; Fiscal Year: 2006
    ..abstract_text> ..
  5. APPLICATION OF NOVEL OPTICAL METHODS TO CELL DYNAMICS
    Alan Verkman; Fiscal Year: 2006
    ..abstract_text> ..
  6. Role of ASL and Airway Glands in CF Lung Disease
    Alan Verkman; Fiscal Year: 2005
    ..Understanding of lung disease mechanisms is of central importance in CF research, having direct implications for development of evaluation of new therapies. ..
  7. Functional Role of Aquaporins in Eye Physiology
    Alan Verkman; Fiscal Year: 2005
    ..If aquaporins are proven to be important, as anticipated, then modulation of aquaporin expression and/or function might provide a new therapeutic strategy for treatment of glaucoma and corneal edema. ..
  8. PHYSIOLOGY AND MOLECULAR BIOLOGY OF LUNG WATER TRANSPORT
    Alan Verkman; Fiscal Year: 2005
    ..abstract_text> ..
  9. Role of ASL and Airway Glands in CF Lung Disease
    Alan Verkman; Fiscal Year: 2004
    ..Understanding of lung disease mechanisms is of central importance in CF research, having direct implications for development of evaluation of new therapies. ..
  10. Application of Novel Optial Methods to Cell Dynamics
    Alan Verkman; Fiscal Year: 2007
    ....
  11. PHYSIOLOGY AND MOLECULAR BIOLOGY OF LUNG WATER TRANSPORT
    Alan Verkman; Fiscal Year: 2007
    ..abstract_text> ..
  12. Functional Role of Aquaporins in Eye Physiology
    Alan Verkman; Fiscal Year: 2007
    ..Together, our studies will establish new roles of aquaporins in the eye with direct implications for new therapies. ..
  13. Role of airway submucosal glands in CF lung disease
    Alan S Verkman; Fiscal Year: 2010
    ..The data will provide a rigorous scientific rationale for use of small-molecules in CF therapy, and establish widely useful assays to prioritize small molecules for clinical development. ..
  14. Functional Role of Aquaporins in Eye Physiology
    Alan Verkman; Fiscal Year: 2009
    ..Together, our studies will establish new roles of aquaporins in the eye with direct implications for new therapies. ..
  15. Role of airway submucosal glands in CF lung disease
    Alan Verkman; Fiscal Year: 2009
    ..The data will provide a rigorous scientific rationale for use of small-molecules in CF therapy, and establish widely useful assays to prioritize small molecules for clinical development. ..
  16. Role of airway submucosal glands in CF lung disease
    Alan Verkman; Fiscal Year: 2007
    ..The data will provide a rigorous scientific rationale for use of small-molecules in CF therapy, and establish widely useful assays to prioritize small molecules for clinical development. ..
  17. Functional Role of Aquaporins in Eye Physiology
    Alan Verkman; Fiscal Year: 2004
    ..If aquaporins are proven to be important, as anticipated, then modulation of aquaporin expression and/or function might provide a new therapeutic strategy for treatment of glaucoma and corneal edema. ..
  18. PHYSIOLOGY AND MOLECULAR BIOLOGY OF LUNG WATER TRANSPORT
    Alan Verkman; Fiscal Year: 2004
    ..abstract_text> ..
  19. APPLICATION OF NOVEL OPTICAL METHODS TO CELL DYNAMICS
    Alan Verkman; Fiscal Year: 1999
    ..These studies will address the hypothesis that the membrane skeleton of these cells is a mobile, dynamic structure. ..
  20. APPLICATION OF NOVEL OPTICAL METHODS TO CELL DYNAMICS
    Alan Verkman; Fiscal Year: 1993
    ..In mitochondria, the hypothesis will be tested that high concentrations of mitochondrial proteins dramatically hinder the motion of metabolite solutes, leading to enzyme organization and metabolite channeling...
  21. PHYSIOLOGY AND MOLECULAR BIOLOGY OF LUNG WATER TRANSPORT
    Alan Verkman; Fiscal Year: 1999
    ..Homozygous transgenic knock-out mice will be generated for AQP3 and AQP5, and characterized in terms of gross phenotype, lung water permeability, clearance of alveolar and interstitial edema, and airway resistance and reactivity. ..
  22. APPLICATION OF NOVEL OPTICAL METHODS TO CELL DYNAMICS
    Alan Verkman; Fiscal Year: 2000
    ..These studies will address the hypothesis that the membrane skeleton of these cells is a mobile, dynamic structure. ..
  23. Role of ASL and Airway Glands in CF Lung Disease
    Alan Verkman; Fiscal Year: 2003
    ..Understanding of lung disease mechanisms is of central importance in CF research, having direct implications for development of evaluation of new therapies. ..
  24. Functional Role of Aquaporins in Eye Physiology
    Alan Verkman; Fiscal Year: 2003
    ..If aquaporins are proven to be important, as anticipated, then modulation of aquaporin expression and/or function might provide a new therapeutic strategy for treatment of glaucoma and corneal edema. ..
  25. Functional Role of Aquaporins in Eye Physiology
    Alan Verkman; Fiscal Year: 2002
    ..If aquaporins are proven to be important, as anticipated, then modulation of aquaporin expression and/or function might provide a new therapeutic strategy for treatment of glaucoma and corneal edema. ..
  26. PHYSIOLOGY AND MOLECULAR BIOLOGY OF LUNG WATER TRANSPORT
    Alan Verkman; Fiscal Year: 2002
    ..Homozygous transgenic knock-out mice will be generated for AQP3 and AQP5, and characterized in terms of gross phenotype, lung water permeability, clearance of alveolar and interstitial edema, and airway resistance and reactivity. ..
  27. PHYSIOLOGY AND MOLECULAR BIOLOGY OF LUNG WATER TRANSPORT
    Alan Verkman; Fiscal Year: 2001
    ..Homozygous transgenic knock-out mice will be generated for AQP3 and AQP5, and characterized in terms of gross phenotype, lung water permeability, clearance of alveolar and interstitial edema, and airway resistance and reactivity. ..
  28. APPLICATION OF NOVEL OPTICAL METHODS TO CELL DYNAMICS
    Alan Verkman; Fiscal Year: 2001
    ..These studies will address the hypothesis that the membrane skeleton of these cells is a mobile, dynamic structure. ..
  29. PHYSIOLOGY AND MOLECULAR BIOLOGY OF LUNG WATER TRANSPORT
    Alan Verkman; Fiscal Year: 2000
    ..Homozygous transgenic knock-out mice will be generated for AQP3 and AQP5, and characterized in terms of gross phenotype, lung water permeability, clearance of alveolar and interstitial edema, and airway resistance and reactivity. ..