Vladislav V Verkhusha

Summary

Affiliation: University of Colorado Health Sciences Center
Country: USA

Publications

  1. ncbi request reprint High stability of Discosoma DsRed as compared to Aequorea EGFP
    Vladislav V Verkhusha
    Department of Pharmacology, University of Colorado Health Sciences Center, Denver, Colorado 80262, USA
    Biochemistry 42:7879-84. 2003
  2. ncbi request reprint Effect of high pressure and reversed micelles on the fluorescent proteins
    Vladislav V Verkhusha
    Department of Pharmacology, C236, University of Colorado Health Sciences Center, 4200 East Ninth Avenue, Denver, CO 80262, USA
    Biochim Biophys Acta 1622:192-5. 2003
  3. ncbi request reprint The molecular properties and applications of Anthozoa fluorescent proteins and chromoproteins
    Vladislav V Verkhusha
    Department of Pharmacology, University of Colorado Health Sciences Center, 4200 East Ninth Avenue, C236, Denver, Colorado 80262, USA
    Nat Biotechnol 22:289-96. 2004
  4. pmc Membrane insertion of the FYVE domain is modulated by pH
    Ju He
    Department of Pharmacology, University of Colorado Denver School of Medicine, Aurora, Colorado 80045, USA
    Proteins 76:852-60. 2009
  5. pmc Molecular mechanism of membrane targeting by the GRP1 PH domain
    Ju He
    Department of Pharmacology, University of Colorado Health Sciences Center, Aurora, CO, USA
    J Lipid Res 49:1807-15. 2008
  6. ncbi request reprint Conversion of the monomeric red fluorescent protein into a photoactivatable probe
    Vladislav V Verkhusha
    Department of Pharmacology, University of Colorado Health Sciences Center, Aurora, CO 80045, USA
    Chem Biol 12:279-85. 2005
  7. pmc pH-dependent binding of the Epsin ENTH domain and the AP180 ANTH domain to PI(4,5)P2-containing bilayers
    Robert A Hom
    Department of Pharmacology, University of Colorado Health Sciences Center, Aurora, CO 80045, USA
    J Mol Biol 373:412-23. 2007
  8. ncbi request reprint Common pathway for the red chromophore formation in fluorescent proteins and chromoproteins
    Vladislav V Verkhusha
    Department of Pharmacology, University of Colorado Health Sciences Center, Denver, CO 80262, USA
    Chem Biol 11:845-54. 2004
  9. ncbi request reprint Three-chromophore FRET microscopy to analyze multiprotein interactions in living cells
    Emilia Galperin
    Department of Pharmacology, University of Colorado Health Sciences Center, Aurora, Colorado 80045, USA
    Nat Methods 1:209-17. 2004
  10. ncbi request reprint Photoswitchable cyan fluorescent protein for protein tracking
    Dmitriy M Chudakov
    Institute of Bioorganic Chemistry RAS, Laboratory of Genes for Regeneration, Miklukho Maklaya 16 10, Moscow 117997, Russia
    Nat Biotechnol 22:1435-9. 2004

Collaborators

Detail Information

Publications22

  1. ncbi request reprint High stability of Discosoma DsRed as compared to Aequorea EGFP
    Vladislav V Verkhusha
    Department of Pharmacology, University of Colorado Health Sciences Center, Denver, Colorado 80262, USA
    Biochemistry 42:7879-84. 2003
    ..Therefore, DsRed can be used as a genetic reporter and advanced population marker with a significantly extended intracellular lifespan...
  2. ncbi request reprint Effect of high pressure and reversed micelles on the fluorescent proteins
    Vladislav V Verkhusha
    Department of Pharmacology, C236, University of Colorado Health Sciences Center, 4200 East Ninth Avenue, Denver, CO 80262, USA
    Biochim Biophys Acta 1622:192-5. 2003
    ..In contrast to the remarkable stability of DsRed, the highest sensitivity of EYFP fluorescence under pressure and in micelles is attributed to its chromophore structure...
  3. ncbi request reprint The molecular properties and applications of Anthozoa fluorescent proteins and chromoproteins
    Vladislav V Verkhusha
    Department of Pharmacology, University of Colorado Health Sciences Center, 4200 East Ninth Avenue, C236, Denver, Colorado 80262, USA
    Nat Biotechnol 22:289-96. 2004
    ..Future studies on the structure of this diverse set of proteins will further enhance their use in animal tissues and as intracellular biosensors...
  4. pmc Membrane insertion of the FYVE domain is modulated by pH
    Ju He
    Department of Pharmacology, University of Colorado Denver School of Medicine, Aurora, Colorado 80045, USA
    Proteins 76:852-60. 2009
    ..Both protonation of the His residues and nonspecific electrostatic contacts stabilize the FYVE domain in the lipid-bound form, promoting its penetration and increasing the membrane residence time...
  5. pmc Molecular mechanism of membrane targeting by the GRP1 PH domain
    Ju He
    Department of Pharmacology, University of Colorado Health Sciences Center, Aurora, CO, USA
    J Lipid Res 49:1807-15. 2008
    ..Together, our results provide new insight into the multivalent mechanism of the membrane targeting and regulation of the GRP1 PH domain...
  6. ncbi request reprint Conversion of the monomeric red fluorescent protein into a photoactivatable probe
    Vladislav V Verkhusha
    Department of Pharmacology, University of Colorado Health Sciences Center, Aurora, CO 80045, USA
    Chem Biol 12:279-85. 2005
    ..PA-mRFP1s were used as protein tags to study the intracellular dynamics of GTPase Rab5...
  7. pmc pH-dependent binding of the Epsin ENTH domain and the AP180 ANTH domain to PI(4,5)P2-containing bilayers
    Robert A Hom
    Department of Pharmacology, University of Colorado Health Sciences Center, Aurora, CO 80045, USA
    J Mol Biol 373:412-23. 2007
    ..The pH sensitivity of the ENTH and ANTH domains is reminiscent to the pH dependency of the FYVE domain suggesting a common regulatory mechanism of membrane anchoring by a subset of the PI-binding domains...
  8. ncbi request reprint Common pathway for the red chromophore formation in fluorescent proteins and chromoproteins
    Vladislav V Verkhusha
    Department of Pharmacology, University of Colorado Health Sciences Center, Denver, CO 80262, USA
    Chem Biol 11:845-54. 2004
    ..Light sensitivity found in the DsRed neutral form, resulting in its instant transformation to the mature red chromophore, could be exploited to accelerate the fluorescence acquisition...
  9. ncbi request reprint Three-chromophore FRET microscopy to analyze multiprotein interactions in living cells
    Emilia Galperin
    Department of Pharmacology, University of Colorado Health Sciences Center, Aurora, Colorado 80045, USA
    Nat Methods 1:209-17. 2004
    ..These data highlight the potential of 3-FRET microscopy in studies of spatial and temporal regulation of signaling processes in living cells...
  10. ncbi request reprint Photoswitchable cyan fluorescent protein for protein tracking
    Dmitriy M Chudakov
    Institute of Bioorganic Chemistry RAS, Laboratory of Genes for Regeneration, Miklukho Maklaya 16 10, Moscow 117997, Russia
    Nat Biotechnol 22:1435-9. 2004
    ..At moderate excitation intensities, PS-CFP can be used as a pH-stable cyan label for protein tagging and fluorescence resonance energy transfer applications...
  11. pmc Fluorescent proteins as biomarkers and biosensors: throwing color lights on molecular and cellular processes
    Olesya V Stepanenko
    Institute of Cytology, Russian Academy of Sciences, St Petersburg, Russia
    Curr Protein Pept Sci 9:338-69. 2008
    ....
  12. pmc Far-red fluorescent tag for protein labelling
    Arkady F Fradkov
    Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry RAS, Miklukho Maklaya 16 10, Moscow 117997, Russia
    Biochem J 368:17-21. 2002
    ..In addition, a possibility of effective fluorescence resonance energy transfer ('FRET') between enhanced yellow FP mutant ('EYFP') and tandem HcRed1 was demonstrated in a protease assay...
  13. ncbi request reprint The place of inactivated actin and its kinetic predecessor in actin folding-unfolding
    Irina M Kuznetsova
    Institute of Cytology, Russian Academy of Science, St Petersburg 194064, Russia
    Biochemistry 41:13127-32. 2002
    ..According to it, the founded essentially unfolded kinetic state is the on-pathway intermediate, while inactivated actin is the off-pathway misfolded state stabilized by aggregation of partially folded macromolecules of protein...
  14. ncbi request reprint Kinetics of actin unfolding induced by guanidine hydrochloride
    Konstantin K Turoverov
    Institute of Cytology, Russian Academy of Science, St Petersburg 194064, Russia
    Biochemistry 41:1014-9. 2002
    ....
  15. ncbi request reprint Engineering of a monomeric green-to-red photoactivatable fluorescent protein induced by blue light
    Nadya G Gurskaya
    Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho Maklaya 16 10, Moscow 117997, Russia
    Nat Biotechnol 24:461-5. 2006
    ..We demonstrate the suitability of Dendra for protein labeling and tracking to quantitatively study dynamics of fibrillarin and vimentin in mammalian cells...
  16. ncbi request reprint Innovation: Photoactivatable fluorescent proteins
    Konstantin A Lukyanov
    Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho Maklaya 16 10, Moscow 117997, Russia
    Nat Rev Mol Cell Biol 6:885-91. 2005
    ..Here, we discuss the properties of the available photoactivatable fluorescent proteins and their potential applications...
  17. pmc Hetero-oligomeric tagging diminishes non-specific aggregation of target proteins fused with Anthozoa fluorescent proteins
    Maria E Bulina
    Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry RAS, Miklukho Maklaya 16 10, Moscow 117997, Russia
    Biochem J 371:109-14. 2003
    ..In addition, heterotetramers appeared to be a unique model for biophysical characterization of Anthozoa FPs in pseudo-monomeric state...
  18. ncbi request reprint Comparative studies on the structure and stability of fluorescent proteins EGFP, zFP506, mRFP1, "dimer2", and DsRed1
    Olesia V Stepanenko
    Institute of Cytology, Russian Academy of Sciences, St Petersburg 194064, Russia
    Biochemistry 43:14913-23. 2004
    ..This means that the quaternary structure, being an important stabilizing factor, does not represent the only circumstance dictating the dramatic variations between fluorescent proteins in their conformational stabilities...
  19. pmc The first mutant of the Aequorea victoria green fluorescent protein that forms a red chromophore
    Alexander S Mishin
    Shemiakin Ovchinnikov Institute of Bioorganic Chemistry, Miklukho Maklaya 16 10, Moscow 117997, Russia
    Biochemistry 47:4666-73. 2008
    ..Model experiments showed that the novel dual-color GFP mutant with green and red emission is suitable for multicolor flow cytometry as an additional color since it is clearly separable from both green and red fluorescent tags...
  20. doi request reprint Solid state yellow and orange lasers for flow cytometry
    Veena Kapoor
    Experimental Transplantation and Immunology Branch, NCI NIH, Bethesda, Maryland 20892, USA
    Cytometry A 73:570-7. 2008
    ..This technology fills an important gap in the laser wavelengths available for flow, now almost any fluorochrome requiring visible light excitation can be accommodated...
  21. ncbi request reprint Expression of recombinant GFP-actin fusion protein in the methylotrophic yeast Pichia pastoris
    Vladislav V Verkhusha
    Center for Molecular Medicine, Building B, Moscow State University, 119899, Moscow, Russia
    FEMS Yeast Res 3:105-11. 2003
    ..The recombinant GFP-actin 5C was used to produce polyclonal antibodies, which had not been reported so far but are extremely needed for immuno-labeling and isolation of wild-type and mutant forms of actin 5C...
  22. ncbi request reprint New lasers for flow cytometry: filling the gaps
    Veena Kapoor
    Nat Methods 4:678-9. 2007