Roxanne Vaughan

Summary

Affiliation: University of North Dakota
Country: USA

Publications

  1. ncbi request reprint Dual incorporation of photoaffinity ligands on dopamine transporters implicates proximity of labeled domains
    R A Vaughan
    Department of Biochemistry and Molecular Biology, University of North Dakota School of Medicine and Health Sciences, Grand Forks, North Dakota, USA
    Mol Pharmacol 59:1157-64. 2001
  2. ncbi request reprint Affinity labeling the dopamine transporter ligand binding site
    Roxanne A Vaughan
    Department of Biochemistry and Molecular Biology, University of North Dakota, School of Medicine and Health Sciences, 501 N Columbia Road, Grand Forks, ND 58203, USA
    J Neurosci Methods 143:33-40. 2005
  3. ncbi request reprint Phosphorylation and regulation of psychostimulant-sensitive neurotransmitter transporters
    Roxanne A Vaughan
    Department of Biochemistry and Molecular Biology, University of North Dakota School of Medicine and Health Sciences, Grand Forks, North Dakota 58201 9037, USA
    J Pharmacol Exp Ther 310:1-7. 2004
  4. ncbi request reprint Localization of cocaine analog [125I]RTI 82 irreversible binding to transmembrane domain 6 of the dopamine transporter
    Roxanne A Vaughan
    Department of Biochemistry and Molecular Biology, University of North Dakota School of Medicine and Health Sciences, Grand Forks, North Dakota 58203 9037, USA
    J Biol Chem 282:8915-25. 2007
  5. doi request reprint Labeling of dopamine transporter transmembrane domain 1 with the tropane ligand N-[4-(4-azido-3-[125I]iodophenyl)butyl]-2beta-carbomethoxy-3beta-(4-chlorophenyl)tropane implicates proximity of cocaine and substrate active sites
    M Laura Parnas
    Department of Biochemistry and Molecular Biology, University of North Dakota School of Medicine and Health Sciences, Grand Forks, ND 58202, USA
    Mol Pharmacol 73:1141-50. 2008
  6. ncbi request reprint Uptake inhibitors but not substrates induce protease resistance in extracellular loop two of the dopamine transporter
    Jon D Gaffaney
    Department of Biochemistry and Molecular Biology, University of North Dakota School of Medicine and Health Sciences, Grand Forks, ND 58203, USA
    Mol Pharmacol 65:692-701. 2004
  7. ncbi request reprint Dopamine transporters are dephosphorylated in striatal homogenates and in vitro by protein phosphatase 1
    James D Foster
    Department of Biochemistry and Molecular Biology, University of North Dakota School of Medicine and Health Sciences, 501 N Columbia Road, Grand Forks, ND 58202, USA
    Brain Res Mol Brain Res 110:100-8. 2003
  8. ncbi request reprint 1-Methyl-4-phenylpyridinium-induced down-regulation of dopamine transporter function correlates with a reduction in dopamine transporter cell surface expression
    Jaturaporn Chagkutip
    Department of Pharmacology, University of North Dakota School of Medicine and Health Sciences, Grand Forks 58202, USA
    Biochem Biophys Res Commun 311:49-54. 2003
  9. ncbi request reprint Dopamine transporters are phosphorylated on N-terminal serines in rat striatum
    James D Foster
    Department of Biochemistry and Molecular Biology, University of North Dakota School of Medicine and Health Sciences, Grand Forks 58202, USA
    J Biol Chem 277:25178-86. 2002
  10. ncbi request reprint Differential effects of dopamine and psychoactive drugs on dopamine transporter phosphorylation and regulation
    Balachandra K Gorentla
    Department of Biochemistry and Molecular Biology, University of North Dakota School of Medicine and Health Sciences, 501 N Columbia Road, Grand Forks, ND 58203, USA
    Neuropharmacology 49:759-68. 2005

Research Grants

Collaborators

Detail Information

Publications15

  1. ncbi request reprint Dual incorporation of photoaffinity ligands on dopamine transporters implicates proximity of labeled domains
    R A Vaughan
    Department of Biochemistry and Molecular Biology, University of North Dakota School of Medicine and Health Sciences, Grand Forks, North Dakota, USA
    Mol Pharmacol 59:1157-64. 2001
    ..These differences in protease sensitivity may indicate the relative proximity of the ligands to the protease site or reflect antagonist-induced conformational changes in the loop related to transport inhibition...
  2. ncbi request reprint Affinity labeling the dopamine transporter ligand binding site
    Roxanne A Vaughan
    Department of Biochemistry and Molecular Biology, University of North Dakota, School of Medicine and Health Sciences, 501 N Columbia Road, Grand Forks, ND 58203, USA
    J Neurosci Methods 143:33-40. 2005
    ..Using these methods we have identified two distinct regions of DAT that interact with multiple structurally related and diverse irreversible ligands, suggesting that these regions may be involved in the formation of ligand binding sites...
  3. ncbi request reprint Phosphorylation and regulation of psychostimulant-sensitive neurotransmitter transporters
    Roxanne A Vaughan
    Department of Biochemistry and Molecular Biology, University of North Dakota School of Medicine and Health Sciences, Grand Forks, North Dakota 58201 9037, USA
    J Pharmacol Exp Ther 310:1-7. 2004
    ....
  4. ncbi request reprint Localization of cocaine analog [125I]RTI 82 irreversible binding to transmembrane domain 6 of the dopamine transporter
    Roxanne A Vaughan
    Department of Biochemistry and Molecular Biology, University of North Dakota School of Medicine and Health Sciences, Grand Forks, North Dakota 58203 9037, USA
    J Biol Chem 282:8915-25. 2007
    ..Cocaine binding near TM6 may thus overlap the dopamine translocation pathway and function to inhibit TM6 structural rearrangements necessary for transport...
  5. doi request reprint Labeling of dopamine transporter transmembrane domain 1 with the tropane ligand N-[4-(4-azido-3-[125I]iodophenyl)butyl]-2beta-carbomethoxy-3beta-(4-chlorophenyl)tropane implicates proximity of cocaine and substrate active sites
    M Laura Parnas
    Department of Biochemistry and Molecular Biology, University of North Dakota School of Medicine and Health Sciences, Grand Forks, ND 58202, USA
    Mol Pharmacol 73:1141-50. 2008
    ..These results directly establish the proximity of TMs 1 and 6 in DAT and suggest that the mechanism of transport inhibition by cocaine involves close interactions with multiple regions of the substrate permeation pathway...
  6. ncbi request reprint Uptake inhibitors but not substrates induce protease resistance in extracellular loop two of the dopamine transporter
    Jon D Gaffaney
    Department of Biochemistry and Molecular Biology, University of North Dakota School of Medicine and Health Sciences, Grand Forks, ND 58203, USA
    Mol Pharmacol 65:692-701. 2004
    ..Together this data provides novel information about inhibitor and substrate interactions at EL2, possibly relating the protease resistant DAT conformation to a mechanism of transport inhibition...
  7. ncbi request reprint Dopamine transporters are dephosphorylated in striatal homogenates and in vitro by protein phosphatase 1
    James D Foster
    Department of Biochemistry and Molecular Biology, University of North Dakota School of Medicine and Health Sciences, 501 N Columbia Road, Grand Forks, ND 58202, USA
    Brain Res Mol Brain Res 110:100-8. 2003
    ..These results demonstrate that DAT is a direct substrate for PP1 in vitro and suggest that PP1 is a major DAT phosphatase in rat striatum...
  8. ncbi request reprint 1-Methyl-4-phenylpyridinium-induced down-regulation of dopamine transporter function correlates with a reduction in dopamine transporter cell surface expression
    Jaturaporn Chagkutip
    Department of Pharmacology, University of North Dakota School of Medicine and Health Sciences, Grand Forks 58202, USA
    Biochem Biophys Res Commun 311:49-54. 2003
    ....
  9. ncbi request reprint Dopamine transporters are phosphorylated on N-terminal serines in rat striatum
    James D Foster
    Department of Biochemistry and Molecular Biology, University of North Dakota School of Medicine and Health Sciences, Grand Forks 58202, USA
    J Biol Chem 277:25178-86. 2002
    ..These results indicate that most or all of the basal and stimulated phosphorylation of DAT in striatal tissue occurs on one or more residues in a group of six serines clustered near the distal end of the cytoplasmic N terminus...
  10. ncbi request reprint Differential effects of dopamine and psychoactive drugs on dopamine transporter phosphorylation and regulation
    Balachandra K Gorentla
    Department of Biochemistry and Molecular Biology, University of North Dakota School of Medicine and Health Sciences, 501 N Columbia Road, Grand Forks, ND 58203, USA
    Neuropharmacology 49:759-68. 2005
    ....
  11. ncbi request reprint Psychoactive substrates stimulate dopamine transporter phosphorylation and down-regulation by cocaine-sensitive and protein kinase C-dependent mechanisms
    Mark A Cervinski
    Department of Biochemistry and Molecular Biology, University of North Dakota School of Medicine and Health Sciences, Grand Forks, North Dakota 58202, USA
    J Biol Chem 280:40442-9. 2005
    ..The finding that DAT phosphorylation is stimulated by amphetamines reveals a previously unknown effect of these drugs that is not produced by cocaine and may be related to reinforcement...
  12. doi request reprint Phorbol ester induced trafficking-independent regulation and enhanced phosphorylation of the dopamine transporter associated with membrane rafts and cholesterol
    James D Foster
    Department of Biochemistry and Molecular Biology, University of North Dakota School of Medicine and Health Sciences, North Dakota 58202, USA
    J Neurochem 105:1683-99. 2008
    ....
  13. pmc Proline-directed phosphorylation of the dopamine transporter N-terminal domain
    Balachandra K Gorentla
    Department of Biochemistry and Molecular Biology, School of Medicine and Health Sciences, University of North Dakota, Grand Forks, North Dakota 58201, USA
    Biochemistry 48:1067-76. 2009
    ....
  14. ncbi request reprint Tyr-95 and Ile-172 in transmembrane segments 1 and 3 of human serotonin transporters interact to establish high affinity recognition of antidepressants
    L Keith Henry
    Department of Pharmacology and Center for Molecular Neuroscience, Vanderbilt University Medical Center, Nashville, TN 37232, USA
    J Biol Chem 281:2012-23. 2006
    ..These studies provided evidence for distinct binding sites coordinating SERT antagonists and revealed a close interaction between TM1 and TM3 differentially targeted by stereoisomers of CIT...
  15. ncbi request reprint Radioiodinated azide and isothiocyanate derivatives of cocaine for irreversible labeling of dopamine transporters: synthesis and covalent binding studies
    John R Lever
    Departments of Radiology, University of Missouri Columbia, Missouri 65212, USA
    Bioconjug Chem 16:644-9. 2005
    ..The utility of the radioligands was demonstrated by their covalent linkage to rat striatal membranes, and immunoprecipitation of a single radiolabeled band at 80 kDa corresponding to the full-length DAT...

Research Grants12

  1. Phosphorylation and Regulation of Dopamine Transporters
    Roxanne Vaughan; Fiscal Year: 2009
    ....
  2. Phosphorylation and Regulation of Dopamine Transporters
    Roxanne Vaughan; Fiscal Year: 2007
    ..4. Identify the endogenous pathways responsible for physiological control of DAT phosphorylation. ..
  3. Affinity Labeling the Dopamine Transporter Active Site
    Roxanne Vaughan; Fiscal Year: 2005
    ..The similarity of DAT to the norepinephrine and serotonin transporters presents the potential for these studies to be applicable to these other transporters. ..
  4. PHOSPHORYLATION AND REGULATION OF DOPAMINE TRANSPORTER
    Roxanne Vaughan; Fiscal Year: 2001
    ..4. Test for related changes in transport of dopamine, binding of antagonists, and surface expression. 5. Identify the endogenous pathways responsible for in vivo control of DAT phosphorylation. ..
  5. Phosphorylation and Regulation of Dopamine Transporters
    Roxanne A Vaughan; Fiscal Year: 2010
    ....