Research Topics
Genomes and Genes | M L VasilSummaryAffiliation: University of Colorado Health Sciences Center Country: USA Publications
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Publications
The fur-regulated gene encoding the alternative sigma factor PvdS is required for iron-dependent expression of the LysR-type regulator ptxR in Pseudomonas aeruginosaM L Vasil
Department of Microbiology, University of Colorado Health Sciences Center, Denver, Colorado 80262, USA
J Bacteriol 180:6784-8. 1998..In contrast, pvdS-mediated iron regulation of ptxR under aerobic conditions is Fur independent...
Architecture of a protein central to iron homeostasis: crystal structure and spectroscopic analysis of the ferric uptake regulatorEhmke Pohl
European Molecular Biology Laboratory, Hamburg Outstation, Notkestr 85, D 22603 Hamburg, Germany
Mol Microbiol 47:903-15. 2003..The combination of these complementary techniques enables us to present a model for the activation and DNA binding of the Fur protein...- Pseudomonas aeruginosa twitching motility-mediated chemotaxis towards phospholipids and fatty acids: specificity and metabolic requirementsRhea M Miller
Department of Microbiology, University of Colorado Denver, Aurora, CO 80045, USA
J Bacteriol 190:4038-49. 2008.... - DNA microarrays in analysis of quorum sensing: strengths and limitationsMichael L Vasil
Department of Microbiology, University of Colorado Health Sciences Center, Denver, Colorado 80262, USA
J Bacteriol 185:2061-5. 2003 - The response of Pseudomonas aeruginosa to iron: genetics, biochemistry and virulenceM L Vasil
Department of Microbiology, University of Colorado Health Sciences Center, Denver, CO 80262, USA
Mol Microbiol 34:399-413. 1999..aeruginosa brings to bear on these questions outside the laboratory, whether it be in soil, in a pipeline, on plants or in the lungs of cystic fibrosis patients... - How we learnt about iron acquisition in Pseudomonas aeruginosa: a series of very fortunate eventsMichael L Vasil
University of Colorado at Denver and Health Sciences Center, 12800 E 19th Avenue RC 1 North P18 9127, Microbiology Mail Stop 8333, P O Box 6511, Aurora, CO 80045, USA
Biometals 20:587-601. 2007..g., citrate) during periods of iron limitation. These and other data to be presented indicate that Fur controlled gene expression in bacteria like P. aeruginosa is considerably more imperative and intricate than previously appreciated... - Characterization of an endoprotease (PrpL) encoded by a PvdS-regulated gene in Pseudomonas aeruginosaP J Wilderman
Department of Microbiology, University of Colorado Health Sciences Center, Denver, Colorado 80262, USA
Infect Immun 69:5385-94. 2001..Finally, PrpL cleaves casein, lactoferrin, transferrin, elastin, and decorin and contributes to PAO1's ability to persist in a rat chronic pulmonary infection model ... - Genetic and biochemical analyses of a eukaryotic-like phospholipase D of Pseudomonas aeruginosa suggest horizontal acquisition and a role for persistence in a chronic pulmonary infection modelP J Wilderman
Department of Microbiology, Campus Box B-175, University of Colorado Health Sciences Center, 4200 East Ninth Avenue, Denver, CO 80262, USA
Mol Microbiol 39:291-303. 2001..Competition studies between a PldA knock-out mutant and the parental wild-type strain indicate that PldA contributes to the ability of P. aeruginosa PAO1 to persist in a chronic pulmonary infection model in rats... - Role of the Pseudomonas aeruginosa oxyR-recG operon in oxidative stress defense and DNA repair: OxyR-dependent regulation of katB-ankB, ahpB, and ahpC-ahpFU A Ochsner
Department of Microbiology, University of Colorado Health Sciences Center, Denver, Colorado 80262, USA
J Bacteriol 182:4533-44. 2000..In conclusion, we present evidence that the oxyR-recG locus is essential for oxidative stress defense and for DNA repair... - Pseudomonas aeruginosa fur overlaps with a gene encoding a novel outer membrane lipoprotein, OmlAU A Ochsner
Department of Microbiology, University of Colorado Health Sciences Center, Denver, Colorado 80262, USA
J Bacteriol 181:1099-109. 1999..A structural role of OmlA in maintaining the cell envelope integrity is proposed... - Genetics and regulation of two distinct haem-uptake systems, phu and has, in Pseudomonas aeruginosaU A Ochsner
Department of Microbiology, University of Colorado Health Sciences Center, Denver 80262, USA
Microbiology 146:185-98. 2000..aeruginosa grown in low-iron media. Taken together, the evidence suggests that the phu and has loci encode two distinct systems required for the acquisition of haem and haemoglobin in P. aeruginosa... - Genetic and physiological characterization of ohr, encoding a protein involved in organic hydroperoxide resistance in Pseudomonas aeruginosaU A Ochsner
Department of Microbiology, University of Colorado Health Sciences Center, Denver, Colorado 80262, USA
J Bacteriol 183:773-8. 2001..An 84-bp portion upstream of the ohr mRNA start site was sufficient for ohr induction by OHPs. Thus, the ohr gene appears to encode an antioxidant enzyme that is not part of the OxyR regulon yet is specifically induced by OHPs... - Molecular characterization of mutants affected in the osmoprotectant-dependent induction of phospholipase C in Pseudomonas aeruginosa PAO1A E Sage
Department of Microbiology, University of Colorado Health Sciences Centre, Denver 80262, USA
Mol Microbiol 23:43-56. 1997..The defects in Tn5G19 are complemented by a DNA fragment from PAO1 that carries the betB gene. The orp gene is located between 0.6 to 6.6 min while betB is located between 10.5 to 12.5 min on the chromosome of PAO1... - Pseudomonas aeruginosa hemolytic phospholipase C suppresses neutrophil respiratory burst activityL S Terada
University of Colorado Health Sciences Center, Denver, Colorado 80262, USA
Infect Immun 67:2371-6. 1999..We conclude that expression of PlcHR by P. aeruginosa suppresses bacterium-induced neutrophil respiratory burst by interfering with a PKC-dependent, non-p38 kinase-dependent pathway... - Gene repression by the ferric uptake regulator in Pseudomonas aeruginosa: cycle selection of iron-regulated genesU A Ochsner
Department of Microbiology, University of Colorado Health Science Center, Denver 80262, USA
Proc Natl Acad Sci U S A 93:4409-14. 1996..The PIGs were mapped on the P.aeruginosa chromosome. Their possible role in iron metabolism and virulence of P. aeruginosa is discussed... - Biochemical and molecular analysis of phospholipase C and phospholipase D activity in mycobacteriaK A Johansen
Department of Microbiology, University of Colorado Health Sciences Center, Denver 80262, USA
Infect Immun 64:3259-66. 1996..These data indicate that PLC and sphingomyelinase activities are associated with the most virulent mycobacterial species, while PLD activity was detected in both virulent and saprophytic strains... - Siderophore-mediated signaling regulates virulence factor production in PseudomonasaeruginosaIain L Lamont
Department of Biochemistry and Centre for Gene Research, University of Otago, P O Box 56, Dunedin, New Zealand
Proc Natl Acad Sci U S A 99:7072-7. 2002..Recognition that a siderophore acts as a signaling molecule has important implications for the understanding of interactions between bacterial cells... - Purification and characterization of mycobacterial phospholipase A: an activity associated with mycobacterial cutinaseSarah K Parker
Department of Pediatrics, University of Colorado Health Sciences Center, Aurora, CO 80045, USA
J Bacteriol 189:4153-60. 2007..All mycobacteria tested had MPLA activity. These studies suggest an alternative use for putative cutinases by the M. tuberculosis group that is likely related to MPLA activity and lipid metabolism... - Iron and Pseudomonas aeruginosa biofilm formationEhud Banin
Department of Microbiology, School of Medicine, University of Washington, Seattle, WA 98195-7242, USA
Proc Natl Acad Sci U S A 102:11076-81. 2005..The two known Fur-controlled small regulatory RNAs (PrrF1 and F2) do not appear to mediate iron control of biofilm development. This information establishes a mechanistic basis for iron control of P. aeruginosa biofilm formation... - The influence of iron on Pseudomonas aeruginosa physiology: a regulatory link between iron and quorum sensingAmanda G Oglesby
University of Colorado Denver, Aurora, Colorado 80231, USA
J Biol Chem 283:15558-67. 2008..aeruginosa... - A novel extracellular phospholipase C of Pseudomonas aeruginosa is required for phospholipid chemotaxisAdam P Barker
Department of Microbiology, 4200 East Ninth Ave, University of Colorado Health Sciences Center, Denver, CO 80262, USA
Mol Microbiol 53:1089-98. 2004..e. plcB). PlcB, but not PlcH or PlcN, is required for directed twitching motility up a gradient of certain kinds of phospholipids. This response shows specificity for the fatty acid moiety of the phospholipid... - Identification of tandem duplicate regulatory small RNAs in Pseudomonas aeruginosa involved in iron homeostasisPaula J Wilderman
Department of Microbiology, University of Colorado Health Sciences Center, Denver, CO 80262, USA
Proc Natl Acad Sci U S A 101:9792-7. 2004..This work suggests that the role of sRNAs in bacterial iron homeostasis may be broad, and approaches similar to those described here may identify these sRNAs in other organisms... - FpvB, an alternative type I ferripyoverdine receptor of Pseudomonas aeruginosaBart Ghysels
Flanders Interuniversity Institute of Biotechnology (VIB6, Laboratory of Microbial Interactions, Vrije Universiteit Brussel, Building E, room 6.6, Pleinlaan 2, B-1050 Brussels, Belgium
Microbiology 150:1671-80. 2004..Inactivation of the fpvB homologues in these strains impaired their capacity to utilize type I ferripyoverdine as a source of iron. Accordingly, introduction of fpvB in trans restored the capacity to utilize type I ferripyoverdine... - Effects of the twin-arginine translocase on secretion of virulence factors, stress response, and pathogenesisUrs A Ochsner
Department of Microbiology, University of Colorado Health Sciences Center, Campus Box B-175, 4200 East Ninth Avenue, Denver, CO 80262, USA
Proc Natl Acad Sci U S A 99:8312-7. 2002..Because the TAT apparatus is well conserved among important bacterial pathogens yet absent in mammalian cells, it represents a potential target for novel antimicrobial compounds... - GeneChip expression analysis of the iron starvation response in Pseudomonas aeruginosa: identification of novel pyoverdine biosynthesis genesUrs A Ochsner
Department of Microbiology, Campus Box B 175, University of Colorado Health Sciences Center, 4200 East Ninth Avenue, Denver, CO 80262, USA
Mol Microbiol 45:1277-87. 2002..The GeneChip proved to be a very useful tool for rapid gene expression analysis and identification of novel genes controlled by Fur or PvdS... - Purification, characterization, and identification of a sphingomyelin synthase from Pseudomonas aeruginosa. PlcH is a multifunctional enzymeChiara Luberto
Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, South Carolina 29425, USA
J Biol Chem 278:32733-43. 2003..This SM synthase did not have reverse transferase activity. In conclusion, the Pseudomonas PlcH also exerts SM synthase activity; therefore, for the first time, we have identified a structural gene for a SM synthase... - Effects of net charge and the number of positively charged residues on the biological activity of amphipathic alpha-helical cationic antimicrobial peptidesZiqing Jiang
Department of Biochemistry and Molecular Genetics, University of Colorado at Denver and Health Sciences Center, Aurora, CO 80045, USA
Biopolymers 90:369-83. 2008..e., the change from +8 to +9 resulted in greater than 32-fold increase in hemolytic activity (250 microg/ml to <7.8 microg/ml, respectively)... - Leakage-free membrane fusion induced by the hydrolytic activity of PlcHR(2), a novel phospholipase C/sphingomyelinase from Pseudomonas aeruginosaL Ruth Montes
Unidad de Biofisica Centro Mixto CSIC UPV EHU, and Departamento de Bioquimica, Universidad del Pais Vasco, Aptdo 644, 48080 Bilbao, Spain
Biochim Biophys Acta 1768:2365-72. 2007.... - Characterization of two distinct phospholipase C enzymes from Burkholderia pseudomalleiSunee Korbsrisate
Department of Immunology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand
Microbiology 153:1907-15. 2007..These data suggested that PLCs contribute, at least in part, to B. pseudomallei virulence and support the view that Plc-1 and Plc-2 are not redundant virulence factors... - Pseudomonas aeruginosa synthesizes phosphatidylcholine by use of the phosphatidylcholine synthase pathwayPaula J Wilderman
Department of Microbiology, University of Colorado Health Sciences Center, 4200 East Ninth Avenue, Denver, CO 80262, USA
J Bacteriol 184:4792-9. 2002..We demonstrate that the PC synthesized by P. aeruginosa PAO1 localized to both the inner and outer membranes, where it is readily accessible to its periplasmic, PC-specific phospholipase D... - Role of peptide hydrophobicity in the mechanism of action of alpha-helical antimicrobial peptidesYuxin Chen
Department of Biochemistry and Molecular Genetics, University of Colorado at Denver and Health Sciences Center, Biomolecular Structure MS 8101, P O Box 6511, Aurora, CO 80045, USA
Antimicrob Agents Chemother 51:1398-406. 2007.... - Comparison of biophysical and biologic properties of alpha-helical enantiomeric antimicrobial peptidesYuxin Chen
Department of Biochemistry and Molecular Genetics, University of Colorado at Denver, Aurora, CO 80045, USA
Chem Biol Drug Des 67:162-73. 2006..The excellent stability of D-enantiomers to trypsin digestion (no proteolysis by trypsin) compared with the rapid breakdown of the L-enantiomers highlights the advantage of the D-enantiomers and their potential as clinical therapeutics... - Role of the Pseudomonas aeruginosa PlcH Tat signal peptide in protein secretion, transcription, and cross-species Tat secretion system compatibilityAleksandra Snyder
Department of Microbiology, Fitzsimons Mail Stop 8333, P.O. Box 6511, 12800 East 19th Ave, Aurora, CO 80045, USA
J Bacteriol 188:1762-74. 2006..Finally, data are presented which indicate that P. aeruginosa PlcH exhibits cross-species compatibility with the Escherichia coli Tat secretion machinery, but only when the E. coli Tat machinery is expressed in a P. aeruginosa host... - A novel class of microbial phosphocholine-specific phospholipases CMartin J Stonehouse
Department of Microbiology, University of Colorado Health Sciences Center, 4200 E. Ninth Ave, Box B-175, Denver, CO 80262, USA
Mol Microbiol 46:661-76. 2002..Inclusive in these data is the conclusion that the members of this PC-PLC and phosphatase family possess a novel mechanism for the recognition and hydrolysis of their respective substrates... - Enhanced Pseudomonas aeruginosa biofilm development mediated by human neutrophilsTravis S Walker
National Jewish Medical and Research Center, D202, 1400 Jackson Street, Denver, CO 80206, USA
Infect Immun 73:3693-701. 2005..When the host fails to eradicate the infection, cellular components from necrotic neutrophils can serve as a biological matrix to facilitate P. aeruginosa biofilm formation... - Pulmonary inflammation induced by Pseudomonas aeruginosa lipopolysaccharide, phospholipase C, and exotoxin A: role of interferon regulatory factor 1Catharina W Wieland
Department of Medicine, University of Colorado Health Sciences Center, Denver, Colorado 80262, USA
Infect Immun 70:1352-8. 2002..These data indicate that different virulence factors from P. aeruginosa induce pulmonary inflammation in vivo and that IRF-1 is involved in some of the cytokine responses to LPS and ETA... - Analysis of a novel prophage-encoded group A Streptococcus extracellular phospholipase A(2)Michal J Nagiec
Center for Human Bacterial Pathogenesis Research, Department of Pathology, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA
J Biol Chem 279:45909-18. 2004..Together, these data provide new information about a novel extracellular enzyme that participates in GAS-human interactions... - Rational design of alpha-helical antimicrobial peptides with enhanced activities and specificity/therapeutic indexYuxin Chen
Department of Biochemistry and Molecular Genetics, University of Colorado Health Sciences Center at Fitzsimons, Aurora, Colorado 80045, USA
J Biol Chem 280:12316-29. 2005....