JAMES J VARANI

Summary

Affiliation: University of Michigan
Country: USA

Publications

  1. ncbi request reprint Human skin in organ culture and human skin cells (keratinocytes and fibroblasts) in monolayer culture for assessment of chemically induced skin damage
    James Varani
    Department of Pathology, The University of Michigan Medical School, Ann Arbor, Michigan 48109, USA
    Toxicol Pathol 35:693-701. 2007
  2. ncbi request reprint Differential expression of an alpha-galactosyl-containing trisaccharide on high- and low-malignant murine sarcoma cells: identification and regulation
    James Varani
    Departments of Pathology, The University of Michigan Medical School, Ann Arbor 48109, USA
    Clin Exp Metastasis 19:1-8. 2002
  3. ncbi request reprint Inhibition of retinoic acid-induced skin irritation in calorie-restricted mice
    James Varani
    Department of Pathology, The University of Michigan, 1301 Catherine Road, Box 0602, Ann Arbor, MI 48109, USA
    Arch Dermatol Res 300:27-35. 2008
  4. pmc Upregulation of calcium-sensing receptor and mitogen-activated protein kinase signalling in the regulation of growth and differentiation in colon carcinoma
    N Bhagavathula
    Department of Pathology, University of Michigan Medical School, Ann Arbor, MI 48109 0602, USA
    Br J Cancer 93:1364-71. 2005
  5. pmc Keratinocyte stimulation of matrix metalloproteinase-1 production and proliferation in fibroblasts: regulation through mitogen-activated protein kinase signalling events
    S E Moon
    Department of Pathology, The University of Michigan Medical School, 1301 Catherine Road, Box 0602, Ann Arbor, Michigan, MI 48109, USA
    Br J Cancer 87:457-64. 2002
  6. ncbi request reprint Epithelial cell invasion of the stroma in human skin organ culture
    James Varani
    Department of Pathology, University of Michigan, Ann Arbor, Michigan 48109, USA
    Front Biosci 9:2989-95. 2004
  7. ncbi request reprint Retinoid-induced epidermal hyperplasia in human skin organ culture: inhibition with soy extract and soy isoflavones
    James Varani
    Department of Pathology, The University of Michigan, Ann Arbor, MI 48109, USA
    Exp Mol Pathol 77:176-83. 2004
  8. ncbi request reprint A novel benzodiazepine selectively inhibits keratinocyte proliferation and reduces retinoid-induced epidermal hyperplasia in organ-cultured human skin
    James Varani
    Department of Pathology, The University of Michigan, 1301 Catherine Rd Box 0602, Ann Arbor, MI 48109, USA
    J Pharmacol Exp Ther 313:56-63. 2005
  9. ncbi request reprint Effects of gadolinium-based magnetic resonance imaging contrast agents on human skin in organ culture and human skin fibroblasts
    James Varani
    Department of Pathology, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA
    Invest Radiol 44:74-81. 2009
  10. ncbi request reprint Antagonism of epidermal growth factor receptor tyrosine kinase ameliorates the psoriatic phenotype in organ-cultured skin
    J Varani
    Department of Pathology, The University of Michigan Medical School, Ann Arbor, 48109, USA
    Skin Pharmacol Physiol 18:123-31. 2005

Research Grants

  1. TOPICAL RETINOIDS FOR DIABETIC FOOT ULCERS
    James Varani; Fiscal Year: 2002
  2. Mechanisms of MMP-3 Action in Acute Lung Injury
    James Varani; Fiscal Year: 2004
  3. TOPICAL RETINOIDS FOR DIABETIC FOOT ULCERS
    James Varani; Fiscal Year: 2003
  4. Mechanisms of MMP-3 Action in Acute Lung Injury
    James Varani; Fiscal Year: 2005
  5. TOPICAL RETINOIDS FOR DIABETIC FOOT ULCERS
    James Varani; Fiscal Year: 2001
  6. SQUAMOUS EPITHELIAL CELL INVASION IN ORGAN CULTURE
    James Varani; Fiscal Year: 2000
  7. SQUAMOUS EPITHELIAL CELL INVASION IN ORGAN CULTURE
    James Varani; Fiscal Year: 1999
  8. Mechanisms of MMP-3 Action in Acute Lung Injury
    James Varani; Fiscal Year: 2003
  9. SQUAMOUS EPITHELIAL CELL INVASION IN ORGAN CULTURE
    James Varani; Fiscal Year: 2001

Collaborators

Detail Information

Publications65

  1. ncbi request reprint Human skin in organ culture and human skin cells (keratinocytes and fibroblasts) in monolayer culture for assessment of chemically induced skin damage
    James Varani
    Department of Pathology, The University of Michigan Medical School, Ann Arbor, Michigan 48109, USA
    Toxicol Pathol 35:693-701. 2007
    ..Taken together, these data suggest that the use of organ-cultured skin in conjunction with cells derived from the skin in monolayer culture may provide an initial approach to screening agents for deleterious changes in skin...
  2. ncbi request reprint Differential expression of an alpha-galactosyl-containing trisaccharide on high- and low-malignant murine sarcoma cells: identification and regulation
    James Varani
    Departments of Pathology, The University of Michigan Medical School, Ann Arbor 48109, USA
    Clin Exp Metastasis 19:1-8. 2002
    ..These studies also identify a mechanism to account for surface carbohydrate differences between the high- and low-malignant cells...
  3. ncbi request reprint Inhibition of retinoic acid-induced skin irritation in calorie-restricted mice
    James Varani
    Department of Pathology, The University of Michigan, 1301 Catherine Road, Box 0602, Ann Arbor, MI 48109, USA
    Arch Dermatol Res 300:27-35. 2008
    ..CR appears to exert a protective effect at the target tissue level rather than by a reduction in pro-inflammatory events, per se...
  4. pmc Upregulation of calcium-sensing receptor and mitogen-activated protein kinase signalling in the regulation of growth and differentiation in colon carcinoma
    N Bhagavathula
    Department of Pathology, University of Michigan Medical School, Ann Arbor, MI 48109 0602, USA
    Br J Cancer 93:1364-71. 2005
    ..They appear to function, at least in part, independently of one another...
  5. pmc Keratinocyte stimulation of matrix metalloproteinase-1 production and proliferation in fibroblasts: regulation through mitogen-activated protein kinase signalling events
    S E Moon
    Department of Pathology, The University of Michigan Medical School, 1301 Catherine Road, Box 0602, Ann Arbor, Michigan, MI 48109, USA
    Br J Cancer 87:457-64. 2002
    ....
  6. ncbi request reprint Epithelial cell invasion of the stroma in human skin organ culture
    James Varani
    Department of Pathology, University of Michigan, Ann Arbor, Michigan 48109, USA
    Front Biosci 9:2989-95. 2004
    ..These observations provide convincing evidence that events thought to be involved in invasion in simple two- and three-dimensional in vitro models are also operative during invasion of intact tissue...
  7. ncbi request reprint Retinoid-induced epidermal hyperplasia in human skin organ culture: inhibition with soy extract and soy isoflavones
    James Varani
    Department of Pathology, The University of Michigan, Ann Arbor, MI 48109, USA
    Exp Mol Pathol 77:176-83. 2004
    ....
  8. ncbi request reprint A novel benzodiazepine selectively inhibits keratinocyte proliferation and reduces retinoid-induced epidermal hyperplasia in organ-cultured human skin
    James Varani
    Department of Pathology, The University of Michigan, 1301 Catherine Rd Box 0602, Ann Arbor, MI 48109, USA
    J Pharmacol Exp Ther 313:56-63. 2005
    ..Together, these data suggest that Bz-423 has the potential to limit the untoward effects associated with topical retinoid treatment, and in addition, may have therapeutic effects against other forms of epidermal hyperplasia...
  9. ncbi request reprint Effects of gadolinium-based magnetic resonance imaging contrast agents on human skin in organ culture and human skin fibroblasts
    James Varani
    Department of Pathology, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA
    Invest Radiol 44:74-81. 2009
    ..The pathogenesis of the disease is largely unknown. The present study addresses potential pathophysiological mechanisms...
  10. ncbi request reprint Antagonism of epidermal growth factor receptor tyrosine kinase ameliorates the psoriatic phenotype in organ-cultured skin
    J Varani
    Department of Pathology, The University of Michigan Medical School, Ann Arbor, 48109, USA
    Skin Pharmacol Physiol 18:123-31. 2005
    ....
  11. ncbi request reprint Calcium, calcium-sensing receptor and growth control in the colonic mucosa
    James Varani
    Department of Pathology, University of Michigan Medical School, 1301 Catherine Street, Ann Arbor, MI 48109, USA
    Histol Histopathol 26:769-79. 2011
    ..Whether a multi-mineral approach will, ultimately, prove to be more effective than Ca²+ alone as a colon cancer chemopreventive agent remains to be seen, but certainly worth investigating...
  12. pmc Decreased collagen production in chronologically aged skin: roles of age-dependent alteration in fibroblast function and defective mechanical stimulation
    James Varani
    Department of Pathology, The University of Michigan, 1301 Catherine Rd Box 0602, Ann Arbor, MI 48109 USA
    Am J Pathol 168:1861-8. 2006
    ..Based on the findings presented here, we conclude that reduced collagen synthesis in chronologically aged skin reflects at least two different underlying mechanisms: cellular fibroblast aging and a lower level of mechanical stimulation...
  13. ncbi request reprint Thiazolidinediones: potential as therapeutics for psoriasis and perhaps other hyperproliferative skin disease
    James Varani
    University of Michigan Medical School, Department of Pathology, 1301 Catherine Road Box 0602, Ann Arbor, MI 48109, USA
    Expert Opin Investig Drugs 15:1453-68. 2006
    ..Other studies are aimed at identifying optimal ways to use these agents in the treatment of hyperplastic skin diseases such as psoriasis...
  14. pmc MDI 301, a non-irritating retinoid, induces changes in human skin that underlie repair
    James Varani
    Department of Pathology, The University of Michigan, 1301 Catherine Road, Box 0602, Ann Arbor, MI 48109, USA
    Arch Dermatol Res 298:439-48. 2007
    ..Potential for efficacy under conditions in which irritation is not observed is a strong rationale for further development of MDI 301 as a skin-repair agent...
  15. pmc Impaired keratinocyte function on matrix metalloproteinase-1 (MMP-1) damaged collagen
    James Varani
    Department of Pathology, University of Michigan Medical School, 1301 Catherine Street, SPC 5602, Ann Arbor, MI 48109, USA
    Arch Dermatol Res 301:497-506. 2009
    ..Based on these findings, we suggest that damage to the underlying collagenous matrix may impede efficient keratinocyte function and retard wound closure...
  16. pmc Attachment and growth of anchorage-dependent cells on a novel, charged-surface microcarrier under serum-free conditions
    J Varani
    Department of Pathology, The University of Michigan, 1301 Catherine Road, P O Box 0602, Ann Arbor, MI, 48109, U S A
    Cytotechnology 28:101-9. 1998
    ..This novel substrate should find application in the industry, especially in processes which depend on viable cell recovery...
  17. ncbi request reprint Reduced fibroblast interaction with intact collagen as a mechanism for depressed collagen synthesis in photodamaged skin
    James Varani
    Department of Pathology, University of Michigan, Ann Arbor, Michigan 48109, USA
    J Invest Dermatol 122:1471-9. 2004
    ..We hypothesize that fibroblasts in severely damaged skin have less interaction with intact collagen and as a result experience a reduction in mechanical tension. Decreased collagen synthesis is (presumed to be) the result...
  18. ncbi request reprint Inhibition of type I procollagen production in photodamage: correlation between presence of high molecular weight collagen fragments and reduced procollagen synthesis
    James Varani
    Department of Pathology, The University of Michigan Medical School, 1301 Catherine Road Box 0602, Ann Arbor, MI 48109, U S A
    J Invest Dermatol 119:122-9. 2002
    ..As these fragments are processed further, there is less inhibition of type I procollagen production...
  19. pmc Inhibition of type I procollagen synthesis by damaged collagen in photoaged skin and by collagenase-degraded collagen in vitro
    J Varani
    Department of Pathology, The University of Michigan Medical School, 1301 Catherine Rd Box 0602, Ann Arbor, MI 48109, USA
    Am J Pathol 158:931-42. 2001
    ....
  20. ncbi request reprint Characteristics of nonmalignant and malignant human prostate in organ culture
    J Varani
    Department of Pathology, University of Michigan Medical School, Ann Arbor 48109, USA
    Lab Invest 79:723-31. 1999
    ..Histological/histochemical features that distinguish nonmalignant and malignant tissue are present in this model...
  21. ncbi request reprint Vitamin A antagonizes decreased cell growth and elevated collagen-degrading matrix metalloproteinases and stimulates collagen accumulation in naturally aged human skin
    J Varani
    Departments of Pathology and Dermatology, The University of Michigan, Medical School, Ann Arbor, MI 48109, USA
    J Invest Dermatol 114:480-6. 2000
    ..In addition, vitamin A treatment reduces matrix metalloproteinase expression and stimulates collagen synthesis in naturally aged, sun-protected skin, as it does in photoaged skin...
  22. pmc Separation of retinoid-induced epidermal and dermal thickening from skin irritation
    James Varani
    Department of Pathology, The University of Michigan, 1301 Catherine Road, Box 0602, Ann Arbor, MI 48109, USA
    Arch Dermatol Res 295:255-62. 2003
    ..Intraperitoneal injection of either 14-all-trans-RA or MDI-301 also resulted in epidermal and dermal thickening. Irritation of skin was not observed in these animals but splenomegaly was prominent in animals treated with either agent...
  23. ncbi request reprint All-trans retinoic acid improves structure and function of diabetic rat skin in organ culture
    James Varani
    Department of Pathology, The University of Michigan, 1301 Catherine Road Box 0602, Ann Arbor, MI 48109, USA
    Diabetes 51:3510-6. 2002
    ..RA also increased proliferation of STZ-D skin fibroblasts (approximately threefold over control; P < 0.05). Together, these data suggest that RA has the capacity to improve structure and function of diabetic skin...
  24. ncbi request reprint Human psoriatic skin in organ culture: comparison with normal skin exposed to exogenous growth factors and effects of an antibody to the EGF receptor
    J Varani
    Department of Pathology, The University of Michigan Medical School, Ann Arbor, MI 48109, USA
    Pathobiology 66:253-9. 1998
    ..They also suggest that organ culture may provide a useful tool with which to elucidate the pathophysiological mechanisms of altered keratinocyte proliferation and differentiation in psoriasis...
  25. ncbi request reprint Heparin-binding epidermal-growth-factor-like growth factor activation of keratinocyte ErbB receptors Mediates epidermal hyperplasia, a prominent side-effect of retinoid therapy
    J Varani
    Department of Pathology, The University of Michigan Medical School, Ann Arbor, Michigan 48109, USA
    J Invest Dermatol 117:1335-41. 2001
    ..Taken together, these data indicate (i) that retinoid-induced epidermal hyperplasia in human skin proceeds through c-erbB, and (ii) that HB-EGF-like growth factor is one of the c-erbB ligands mediating this effect...
  26. pmc All-trans-retinoic acid suppresses matrix metalloproteinase activity and increases collagen synthesis in diabetic human skin in organ culture
    Humaira Lateef
    Department of Pathology, The University of Michigan, 1301 Catherine Road, Ann Arbor, MI 48109, USA
    Am J Pathol 165:167-74. 2004
    ..Together, these data suggest that RA has the capacity to improve structure and function of diabetic skin, and that a major effect is on reduction of collagen-degrading MMPs...
  27. ncbi request reprint Vascular expression of matrix metalloproteinase-13 (collagenase-3) in basal cell carcinoma
    Yukari Hattori
    Department of Pathology, The University of Michigan, Ann Arbor 48109, USA
    Exp Mol Pathol 74:230-7. 2003
    ..These data indicate that endothelial cells in the skin are a source of MMP-13 and that enzyme expression is upregulated under conditions that promote endothelial cell growth and vascular differentiation...
  28. pmc MDI 301, a nonirritating retinoid, improves abrasion wound healing in damaged/atrophic skin
    Roscoe L Warner
    Department of Pathology, University of Michigan, Ann Arbor, MI 48109, USA
    Wound Repair Regen 16:117-24. 2008
    ..In all three models, the level of type I collagen was increased and MMP levels were decreased by MDI 301. In all three models, skin irritation during the retinoid-treatment phase was virtually nonexistent...
  29. ncbi request reprint PADMA 28: a multi-component herbal preparation with retinoid-like dermal activity but without epidermal effects
    Muhammad Nadeem Aslam
    Department of Pathology, The University of Michigan Medical School, Ann Arbor, Michigan 48109, USA
    J Invest Dermatol 124:524-9. 2005
    ....
  30. ncbi request reprint Retinoid-induced epidermal hyperplasia is mediated by epidermal growth factor receptor activation via specific induction of its ligands heparin-binding EGF and amphiregulin in human skin in vivo
    Laure Rittie
    Department of Dermatology, University of Michigan Medical School, University of Michigan, Ann Arbor, Michigan 48109 0609, USA
    J Invest Dermatol 126:732-9. 2006
    ..These data demonstrate the central role of EGFR activation in retinoid-induced epidermal hyperplasia, and suggest that EGFR inhibitors can mitigate retinoid-induced scaling...
  31. pmc Amphiregulin and epidermal hyperplasia: amphiregulin is required to maintain the psoriatic phenotype of human skin grafts on severe combined immunodeficient mice
    Narasimharao Bhagavathula
    Department of Pathology, University of Michigan, 1301 Catherine Rd, Box 0602, Ann Arbor, MI 48109, USA
    Am J Pathol 166:1009-16. 2005
    ..These data also suggest that the hyperplastic response occurring in nonpsoriatic human skin on transplantation to the SCID mouse is mediated, in large part, by amphiregulin...
  32. ncbi request reprint Matrix metalloproteinase expression in basal cell carcinoma: relationship between enzyme profile and collagen fragmentation pattern
    Taskin Yucel
    Department of Otolaryngology Head and Neck Surgery, The University of Michigan Medical School, Ann Arbor, MI 48109, USA
    Exp Mol Pathol 79:151-60. 2005
    ....
  33. ncbi request reprint Rosiglitazone inhibits proliferation, motility, and matrix metalloproteinase production in keratinocytes
    Narasimharao Bhagavathula
    Department of Pathology, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA
    J Invest Dermatol 122:130-9. 2004
    ....
  34. ncbi request reprint Matrix metalloproteinase-1 is the major collagenolytic enzyme responsible for collagen damage in UV-irradiated human skin
    Meghan Brennan
    Department of Pathology, University of Michigan Medical School, Ann Arbor, MI 48109, USA
    Photochem Photobiol 78:43-8. 2003
    ..These findings strongly implicate MMP-1 rather than MMP-13 as the major collagenolytic enzyme responsible for collagen damage in photoaging...
  35. ncbi request reprint Pomegranate as a cosmeceutical source: pomegranate fractions promote proliferation and procollagen synthesis and inhibit matrix metalloproteinase-1 production in human skin cells
    Muhammad Nadeem Aslam
    Department of Pathology, The University of Michigan Medical School, 1301 Catherine Road Box 0602, Ann Arbor, MI 48109, USA
    J Ethnopharmacol 103:311-8. 2006
    ....
  36. ncbi request reprint Marasmius oreades lectin induces renal thrombotic microangiopathic lesions
    Roscoe L Warner
    Department of Pathology, University of Michigan Medical School, Ann Arbor, MI 48109 0602, USA
    Exp Mol Pathol 77:77-84. 2004
    ..We suggest that MOA-induced glomerular microangiopathic injury could be used to study the pathophysiology of endothelial cell injury as related to glomerular microangiopathic injury...
  37. ncbi request reprint Matrix metalloproteinases in acute inflammation: induction of MMP-3 and MMP-9 in fibroblasts and epithelial cells following exposure to pro-inflammatory mediators in vitro
    Roscoe L Warner
    The Department of Pathology, The University of Michigan, Ann Arbor, MI 48109, USA
    Exp Mol Pathol 76:189-95. 2004
    ..The observation that resident cells in the tissue parenchyma can elaborate MMPs in direct response to pro-inflammatory stimuli provides insight into possible mechanisms by which tissue damage occurs in acute inflammation...
  38. ncbi request reprint Pretreatment of diabetic rats with lipoic acid improves healing of subsequently-induced abrasion wounds
    Humaira Lateef
    Department of Pathology, The University of Michigan, 1301 Catherine Road 0602, Ann Arbor, MI 48109, USA
    Arch Dermatol Res 297:75-83. 2005
    ..These findings suggest that prophylactic use of lipoic acid might be useful in preventing the development of non-healing skin ulcers from minor traumas in at-risk skin...
  39. ncbi request reprint Sustained high-yield production of recombinant proteins in transiently transfected COS-7 cells grown on trimethylamine-coated (hillex) microcarrier beads
    Randall N Knibbs
    Department of Pathology, University of Michigan, Ann Arbor 48109 0602, USA
    Biotechnol Prog 19:9-13. 2003
    ..Thus, microcarrier-based transient transfection yields quantities of recombinant proteins with a significant savings of time and reagents over monolayer culture...
  40. pmc Collagenolytic activity is suppressed in organ-cultured human skin exposed to a gadolinium-based MRI contrast agent
    Patricia A Perone
    Department of Pathology, University of Michigan Medical School, Ann Arbor, MI 48109, USA
    Invest Radiol 45:42-8. 2010
    ..The objective of the present study was to determine whether collagenolytic activity was concomitantly up-regulated with increased enzyme...
  41. pmc Growth-inhibitory effects of a mineralized extract from the red marine algae, Lithothamnion calcareum, on Ca(2+)-sensitive and Ca(2+)-resistant human colon carcinoma cells
    Muhammad Nadeem Aslam
    Department of Pathology, University of Michigan Medical School, 1301 Catherine Street, SPC 5602, Ann Arbor, MI 48109, USA
    Cancer Lett 283:186-92. 2009
    ..4mM). However, with cells that are resistant to Ca2+ alone, the extract was still able to reduce proliferation and stimulate differentiation...
  42. pmc Recombinant collagen for animal product-free dextran microcarriers
    Michael K Dame
    Department of Pathology, The University of Michigan, Ann Arbor, MI 48109, USA
    In Vitro Cell Dev Biol Anim 44:407-14. 2008
    ..We are confident, based on these findings, that a fully recombinant substitute could replace gelatin in current microcarrier preparations without losing the cell growth benefits provided by the native protein...
  43. pmc Collagen fragmentation promotes oxidative stress and elevates matrix metalloproteinase-1 in fibroblasts in aged human skin
    Gary J Fisher
    Department of Dermatology, Medical School, University of Michigan, Ann Arbor, Michigan 48109 5609, USA
    Am J Pathol 174:101-14. 2009
    ..These data extend the current understanding of the oxidative theory of aging beyond a cellular-centric view to include extracellular matrix and the critical role that connective tissue microenvironment plays in the biology of aging...
  44. pmc The Göttingen minipig for assessment of retinoid efficacy in the skin: comparison of results from topically treated animals with results from organ-cultured skin
    Michael K Dame
    Department of Pathology, University of Michigan Medical School, 1301 Catherine St, SPC 5602, Ann Arbor, MI, 48109 5602, USA
    In Vitro Cell Dev Biol Anim 45:551-7. 2009
    ..The in vitro organ culture approach may provide a cost-effective alternative model to that of the intact animal for skin retinoid testing...
  45. pmc Human colon tissue in organ culture: preservation of normal and neoplastic characteristics
    Michael K Dame
    Department of Pathology, University of Michigan Medical School, Ann Arbor, MI 48109, USA
    In Vitro Cell Dev Biol Anim 46:114-22. 2010
    ..E-cadherin was seen at the cell surface (similar to normal tissue), but in some places, there was diffuse cytoplasmic staining. Finally, intense cytoplasmic and nuclear β-catenin staining was observed in cultured neoplastic tissue...
  46. pmc A mineral-rich red algae extract inhibits polyp formation and inflammation in the gastrointestinal tract of mice on a high-fat diet
    Muhammad N Aslam
    Department of Pathology, University of Michigan Medical School, 1301 Catherine Street, SPC 5602, Ann Arbor, MI 48109, USA
    Integr Cancer Ther 9:93-9. 2010
    ..Both were significantly reduced in animals on the low-fat diet and animals on the extract-supplemented HFWD.These data suggest that the mineral-rich algae extract may provide a novel approach to chemoprevention in the colon...
  47. pmc Looking older: fibroblast collapse and therapeutic implications
    Gary J Fisher
    Department of Dermatology, University of Michigan, Medical Science I Bldg, R6447, 1150 W Medical Center Dr, Ann Arbor, MI 48109 0609, USA
    Arch Dermatol 144:666-72. 2008
    ..Treatments that stimulate production of new, nonfragmented collagen should provide substantial improvement to the appearance and health of aged skin...
  48. pmc Human colon tissue in organ culture: calcium and multi-mineral-induced mucosal differentiation
    Michael K Dame
    Department of Pathology, University of Michigan Medical School, 1301 Catherine Street, Ann Arbor, MI 48109 5602, USA
    In Vitro Cell Dev Biol Anim 47:32-8. 2011
    ....
  49. pmc A mineral-rich extract from the red marine algae Lithothamnion calcareum preserves bone structure and function in female mice on a Western-style diet
    Muhammad Nadeem Aslam
    Department of Pathology, University of Michigan Medical School, Ann Arbor, MI 48109, USA
    Calcif Tissue Int 86:313-24. 2010
    ..Female mice on the mineral-supplemented HFWD had higher plasma levels of TRAP than mice of the other groups. There were no differences in the other two markers. Male mice showed little diet-specific differences by micro-CT...
  50. ncbi request reprint Establishment and characteristics of Gottingen minipig skin in organ culture and monolayer cell culture: relevance to drug safety testing
    Michael K Dame
    Department of Pathology, The University of Michigan Medical School, 1150 W Medical Center Dr, Box 0602, Ann Arbor, MI 48109, USA
    In Vitro Cell Dev Biol Anim 44:245-52. 2008
    ..Taken together, these data suggest that the skin from the Gottingen minipig can be used as a surrogate for human skin in ex vivo skin safety studies...
  51. ncbi request reprint Collagen degradation in aged/photodamaged skin in vivo and after exposure to matrix metalloproteinase-1 in vitro
    Suzanne E G Fligiel
    Department of Pathology, The University of Michigan, Ann Arbor, Michigan 48109, USA
    J Invest Dermatol 120:842-8. 2003
    ..We suggest that collagen fragmentation in vivo could underlie the loss of collagen synthesis in photodamaged skin and, to a lesser extent perhaps, in aged skin...
  52. ncbi request reprint A stepwise method for the isolation of endothelial cells and smooth muscle cells from individual canine coronary arteries
    Michael K Dame
    Department of Pathology, University of Michigan Medical School, 4224 Medical Science I Building, 1301 Catherine Street, Ann Arbor, Michigan 48109 0602, USA
    In Vitro Cell Dev Biol Anim 39:402-6. 2003
    ..Capacity to isolate endothelial cells and smooth muscle cells from individual canine coronary arteries should prove useful in the study of coronary artery disease...
  53. ncbi request reprint Mechanisms of photoaging and chronological skin aging
    Gary J Fisher
    Department of Dermatology, University of Michigan, Ann Arbor, MI 48109, USA
    Arch Dermatol 138:1462-70. 2002
    ..This article reviews our current understanding and presents new data about the molecular pathways that mediate skin damage by UV irradiation and by the passage of time...
  54. ncbi request reprint In vivo stimulation of de novo collagen production caused by cross-linked hyaluronic acid dermal filler injections in photodamaged human skin
    Frank Wang
    Department of Dermatology, University of Michigan Medical School, Ann Arbor, MI 48109, USA
    Arch Dermatol 143:155-63. 2007
    ..To determine whether endogenous synthesis of new extracellular matrix may contribute to the degree and duration of clinical benefits derived from cross-linked hyaluronic acid dermal filler injections...
  55. ncbi request reprint BP-1107 [{2-[4-(2,4-dioxo-thiazolidin-5-ylmethyl)-phenoxy]-ethyl}-methyl-amide]: a novel synthetic thiazolidinedione that inhibits epidermal hyperplasia in psoriatic skin-severe-combined immunodeficient mouse transplants after topical application
    Narasimharao Bhagavathula
    Department of Pathology, The University of Michigan, 1301 Catherine Rd, Box 0602, Ann Arbor, MI 48109, USA
    J Pharmacol Exp Ther 315:996-1004. 2005
    ..Concentrations of BP-1107 that effectively inhibit keratinocyte function have no detrimental effect on dermal fibroblasts. These data suggest that effective topical antipsoriatic therapy may be provided with this agent...
  56. ncbi request reprint Topical pretreatment of diabetic rats with all-trans retinoic acid improves healing of subsequently induced abrasion wounds
    Humaira Lateef
    Department of Pathology, University of Michigan, 1301 Catherine Road Box 0602, Ann Arbor, MI 48109, USA
    Diabetes 54:855-61. 2005
    ..In light of these findings, we suggest that prophylactic use of retinoid-containing preparations might be useful in preventing the development of nonhealing skin ulcers resultant from minor traumas in at-risk skin...
  57. ncbi request reprint Matrix metalloproteinase expression in normal skin associated with basal cell carcinoma and in distal skin from the same patients
    Nathan Monhian
    Department of Otolaryngology Head and Neck Surgery, University of Michigan Medical School, Ann Arbor, 48109, USA
    Arch Facial Plast Surg 7:238-43. 2005
    ....
  58. pmc A combination of curcumin and ginger extract improves abrasion wound healing in corticosteroid-impaired hairless rat skin
    Narasimharao Bhagavathula
    Department of Pathology, The University of Michigan Medical School, Ann Arbor, Michigan 48109, USA
    Wound Repair Regen 17:360-6. 2009
    ....
  59. pmc Regulation of collagen turnover in human skin fibroblasts exposed to a gadolinium-based contrast agent
    Narasimharao Bhagavathula
    Department of Pathology, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA
    Invest Radiol 44:433-9. 2009
    ....
  60. ncbi request reprint 7-Chloro-5-(4-hydroxyphenyl)-1-methyl-3-(naphthalen-2-ylmethyl)-4,5-dihydro-1H-benzo[b][1,4]diazepin-2(3H)-one (Bz-423), a benzodiazepine, suppresses keratinocyte proliferation and has antipsoriatic activity in the human skin-severe, combined immunodefici
    Narasimharao Bhagavathula
    Department of Pathology, The University of Michigan, 1301 Catherine Rd, Box 0602, Ann Arbor, MI 48109, USA
    J Pharmacol Exp Ther 324:938-47. 2008
    ....
  61. ncbi request reprint Matrix metalloproteinases and matrix metalloproteinase inhibitors in acute lung injury
    Suzanne E G Fligiel
    Department of Pathology, The University of Michigan, Ann Arbor, 48109, USA
    Hum Pathol 37:422-30. 2006
    ..On the other hand, the presence of detectable MMP-1 and/or MMP-3 is an indicator of more ominous disease progression...
  62. ncbi request reprint Regulation of E-cadherin and beta-catenin by Ca2+ in colon carcinoma is dependent on calcium-sensing receptor expression and function
    Narasimharao Bhagavathula
    Department of Pathology, University of Michigan Medical School, Ann Arbor, MI 48109, USA
    Int J Cancer 121:1455-62. 2007
    ..Where CaSR expression was high, strong surface staining for E-cadherin and beta-catenin was observed. Where CaSR expression was reduced, beta-catenin surface expression was likewise reduced...
  63. ncbi request reprint Improvement of naturally aged skin with vitamin A (retinol)
    Reza Kafi
    Department of Dermatology, University of Michigan Medical School, Ann Arbor, MI 48109, USA
    Arch Dermatol 143:606-12. 2007
    ..To evaluate the effectiveness of topical retinol (vitamin A) in improving the clinical signs of naturally aged skin...
  64. pmc Role of metalloelastase in a model of allergic lung responses induced by cockroach allergen
    Roscoe L Warner
    Department of Pathology, The University of Michigan, 1301 Catherine Rd, Box 0602, Ann Arbor, MI 48109, USA
    Am J Pathol 165:1921-30. 2004
    ..Alterations in the levels of chemotactic factors and other proinflammatory cytokines in the MMP-12-deficient mice may underlie the decrease in leukocyte recruitment into inflamed lungs...
  65. pmc Matrix metalloproteinase-3 (stromelysin-1) in acute inflammatory tissue injury
    Kamalakar C Nerusu
    Department of Pathology, The University of Michigan Medical School, 1301 Catherine Road Box 0602, Ann Arbor, MI 48109, USA
    Exp Mol Pathol 83:169-76. 2007
    ..There was also a relationship between tissue injury and influx of neutrophils into the BAL or PL fluid. Taken together, these data demonstrate an important role for MMP-3 in acute inflammatory tissue injury...

Research Grants12

  1. TOPICAL RETINOIDS FOR DIABETIC FOOT ULCERS
    James Varani; Fiscal Year: 2002
    ..abstract_text> ..
  2. Mechanisms of MMP-3 Action in Acute Lung Injury
    James Varani; Fiscal Year: 2004
    ..These studies will provide an overall understanding of the mechanism(s) by which MMP-3 contributes to acute lung injury. ..
  3. TOPICAL RETINOIDS FOR DIABETIC FOOT ULCERS
    James Varani; Fiscal Year: 2003
    ..abstract_text> ..
  4. Mechanisms of MMP-3 Action in Acute Lung Injury
    James Varani; Fiscal Year: 2005
    ..These studies will provide an overall understanding of the mechanism(s) by which MMP-3 contributes to acute lung injury. ..
  5. TOPICAL RETINOIDS FOR DIABETIC FOOT ULCERS
    James Varani; Fiscal Year: 2001
    ..abstract_text> ..
  6. SQUAMOUS EPITHELIAL CELL INVASION IN ORGAN CULTURE
    James Varani; Fiscal Year: 2000
    ..abstract_text> ..
  7. SQUAMOUS EPITHELIAL CELL INVASION IN ORGAN CULTURE
    James Varani; Fiscal Year: 1999
    ..abstract_text> ..
  8. Mechanisms of MMP-3 Action in Acute Lung Injury
    James Varani; Fiscal Year: 2003
    ..These studies will provide an overall understanding of the mechanism(s) by which MMP-3 contributes to acute lung injury. ..
  9. SQUAMOUS EPITHELIAL CELL INVASION IN ORGAN CULTURE
    James Varani; Fiscal Year: 2001
    ..abstract_text> ..