R D Vale

Summary

Affiliation: University of California
Country: USA

Publications

  1. pmc Molecular requirements for actin-based lamella formation in Drosophila S2 cells
    Stephen L Rogers
    Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94107, USA
    J Cell Biol 162:1079-88. 2003
  2. pmc Searching for kinesin's mechanical amplifier
    R D Vale
    Howard Hughes Medical Institute, and Department of Cellular and Molecular Pharmacology, University of California, San Francisco 94143, USA
    Philos Trans R Soc Lond B Biol Sci 355:449-57. 2000
  3. pmc AAA proteins. Lords of the ring
    R D Vale
    Howard Hughes Medical Institute and the Department of Cellular and Molecular Pharmacology, University of California, San Francisco, California 94143, USA
    J Cell Biol 150:F13-9. 2000
  4. ncbi request reprint The way things move: looking under the hood of molecular motor proteins
    R D Vale
    Howard Hughes Medical Institute and Department of Cellular and Molecular Pharmacology, University of California, 513 Parnassus Avenue, San Francisco, CA 94143, USA
    Science 288:88-95. 2000
  5. ncbi request reprint Millennial musings on molecular motors
    R D Vale
    Howard Hughes Medical Institute and the Dept of Cellular and Molecular Pharmacology, University of California, San Francisco, CA 94143, USA
    Trends Cell Biol 9:M38-42. 1999
  6. ncbi request reprint The molecular motor toolbox for intracellular transport
    Ronald D Vale
    Department of Cellular and Molecular Pharmacology, Howard Hughes Medical Institute, University of California, San Francisco, 513 Parnassus Avenue, San Francisco, CA 94143, USA
    Cell 112:467-80. 2003
  7. ncbi request reprint The design plan of kinesin motors
    R D Vale
    Howard Hughes Medical Institute, University of California, San Francisco 94143, USA
    Annu Rev Cell Dev Biol 13:745-77. 1997
  8. ncbi request reprint Plasma membrane compartmentalization in yeast by messenger RNA transport and a septin diffusion barrier
    P A Takizawa
    Department of Cellular and Molecular Pharmacology, Howard Hughes Medical Institute, University of California, San Francisco, CA 94143, USA
    Science 290:341-4. 2000
  9. pmc The myosin motor, Myo4p, binds Ash1 mRNA via the adapter protein, She3p
    P A Takizawa
    Department of Cellular and Molecular Pharmacology and Howard Hughes Medical Institute, University of California, San Francisco, CA 94143 0450, USA
    Proc Natl Acad Sci U S A 97:5273-8. 2000
  10. ncbi request reprint Katanin, a microtubule-severing protein, is a novel AAA ATPase that targets to the centrosome using a WD40-containing subunit
    J J Hartman
    Department of Cellular and Molecular Pharmacology, University of California, San Francisco 94143, USA
    Cell 93:277-87. 1998

Collaborators

Detail Information

Publications89

  1. pmc Molecular requirements for actin-based lamella formation in Drosophila S2 cells
    Stephen L Rogers
    Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94107, USA
    J Cell Biol 162:1079-88. 2003
    ..Our results have identified an essential set of proteins involved in actin dynamics during lamella formation in Drosophila S2 cells...
  2. pmc Searching for kinesin's mechanical amplifier
    R D Vale
    Howard Hughes Medical Institute, and Department of Cellular and Molecular Pharmacology, University of California, San Francisco 94143, USA
    Philos Trans R Soc Lond B Biol Sci 355:449-57. 2000
    ..Thus, kinesin and myosin have evolved unique mechanical elements that amplify small, nucleotide-dependent conformational changes that occur in their similar catalytic cores...
  3. pmc AAA proteins. Lords of the ring
    R D Vale
    Howard Hughes Medical Institute and the Department of Cellular and Molecular Pharmacology, University of California, San Francisco, California 94143, USA
    J Cell Biol 150:F13-9. 2000
  4. ncbi request reprint The way things move: looking under the hood of molecular motor proteins
    R D Vale
    Howard Hughes Medical Institute and Department of Cellular and Molecular Pharmacology, University of California, 513 Parnassus Avenue, San Francisco, CA 94143, USA
    Science 288:88-95. 2000
    ..This modular design has given rise to a remarkable diversity of kinesin and myosin motors whose motile properties are optimized for performing distinct biological functions...
  5. ncbi request reprint Millennial musings on molecular motors
    R D Vale
    Howard Hughes Medical Institute and the Dept of Cellular and Molecular Pharmacology, University of California, San Francisco, CA 94143, USA
    Trends Cell Biol 9:M38-42. 1999
    ..This article explores how these remarkable protein machines might have evolved and what roles they could play in biological and medical research in the coming decades...
  6. ncbi request reprint The molecular motor toolbox for intracellular transport
    Ronald D Vale
    Department of Cellular and Molecular Pharmacology, Howard Hughes Medical Institute, University of California, San Francisco, 513 Parnassus Avenue, San Francisco, CA 94143, USA
    Cell 112:467-80. 2003
    ..Remarkably, fungi, parasites, plants, and animals have distinct subsets of Toolbox motors in their genomes, suggesting an underlying diversity of strategies for intracellular transport...
  7. ncbi request reprint The design plan of kinesin motors
    R D Vale
    Howard Hughes Medical Institute, University of California, San Francisco 94143, USA
    Annu Rev Cell Dev Biol 13:745-77. 1997
    ..We also discuss how the kinesin motor domain uses chemical energy from ATP hydrolysis to move along microtubules...
  8. ncbi request reprint Plasma membrane compartmentalization in yeast by messenger RNA transport and a septin diffusion barrier
    P A Takizawa
    Department of Cellular and Molecular Pharmacology, Howard Hughes Medical Institute, University of California, San Francisco, CA 94143, USA
    Science 290:341-4. 2000
    ..These results indicate that yeast creates distinct plasma membrane compartments, as has been described in neurons and epithelial cells...
  9. pmc The myosin motor, Myo4p, binds Ash1 mRNA via the adapter protein, She3p
    P A Takizawa
    Department of Cellular and Molecular Pharmacology and Howard Hughes Medical Institute, University of California, San Francisco, CA 94143 0450, USA
    Proc Natl Acad Sci U S A 97:5273-8. 2000
    ..These results suggest that She3p acts as an adapter protein that docks the myosin motor onto an Ash1-She2p ribonucleoprotein complex...
  10. ncbi request reprint Katanin, a microtubule-severing protein, is a novel AAA ATPase that targets to the centrosome using a WD40-containing subunit
    J J Hartman
    Department of Cellular and Molecular Pharmacology, University of California, San Francisco 94143, USA
    Cell 93:277-87. 1998
    ..These results indicate katanin's activities are segregated into a subunit (p60) that possesses enzymatic activity and a subunit (p80) that targets the enzyme to the centrosome...
  11. ncbi request reprint Crystal structure of the motor domain of the kinesin-related motor ncd
    E P Sablin
    Department of Biochemistry Biophysics, University of California, San Francisco, California 94143, USA
    Nature 380:555-9. 1996
    ..We propose a general model for converting a common gamma-phosphate-sensing mechanism into opposite polarities of movement for kinesin and ncd...
  12. pmc EPR spectroscopy shows a microtubule-dependent conformational change in the kinesin switch 1 domain
    Nariman Naber
    Department of Biochemistry and Biophysics, Department of Cellular and Molecular Pharmacology, and Cardiovascular Research Institute, University of California, San Francisco, CA 94143, USA
    Biophys J 84:3190-6. 2003
    ..For kinesin.ADP.MT, a van't Hoff plot gave DeltaH degrees = -96 kJ/mol implying that the conformational change was extensive. We conclude there is a conformational change in the switch 1-alpha3-helix domain when kinesin binds to MTs...
  13. ncbi request reprint Cloning and localization of a conventional kinesin motor expressed exclusively in neurons
    J Niclas
    Department of Pharmacology, University of California, San Francisco 94143
    Neuron 12:1059-72. 1994
    ..These results demonstrate that mammalian neuronal tissue contains two conventional kinesin motors which may serve distinct functions in microtubule-based transport...
  14. pmc Isolation of a ribonucleoprotein complex involved in mRNA localization in Drosophila oocytes
    J E Wilhelm
    Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, California 94143, USA
    J Cell Biol 148:427-40. 2000
    ..We propose that Exu is a core component of a large protein complex involved in localizing mRNAs both within nurse cells and the developing oocyte...
  15. ncbi request reprint Single-molecule behavior of monomeric and heteromeric kinesins
    D W Pierce
    Howard Hughes Medical Institute, Department of Cellular and Molecular Pharmacology, University of California, San Francisco 94143, USA
    Biochemistry 38:5412-21. 1999
    ..Conventional kinesin's unusual processivity may be required for efficient transport of protein complexes that cannot carry multiple motors...
  16. pmc Crystal structure of the kinesin motor domain reveals a structural similarity to myosin
    F J Kull
    Department of Biochemistry Biophysics, University of California, San Francisco, California 94143, USA
    Nature 380:550-5. 1996
    ....
  17. ncbi request reprint Circularization of mRNA by eukaryotic translation initiation factors
    S E Wells
    Department of Molecular and Cell Biology, University of California, Berkeley 94720, USA
    Mol Cell 2:135-40. 1998
    ..Our results suggest that formation of circular mRNA by translation factors could contribute to the control of mRNA expression in the eukaryotic cell...
  18. ncbi request reprint The directional preference of kinesin motors is specified by an element outside of the motor catalytic domain
    R B Case
    Department of Pharmacology, University of California, San Francisco 94143, USA
    Cell 90:959-66. 1997
    ..We propose that a region adjacent to the catalytic domain serves as a mechanical transducer that determines directionality...
  19. ncbi request reprint The case for a common ancestor: kinesin and myosin motor proteins and G proteins
    F J Kull
    Howard Hughes Medical Institute, University of California, San Francisco, USA
    J Muscle Res Cell Motil 19:877-86. 1998
    ..The different topologies can be accounted for by unique genetic insertions that add to the edge of a progenitor protein structure and do not disrupt the hydrophobic core...
  20. ncbi request reprint Microtubule interaction site of the kinesin motor
    G Woehlke
    Howard Hughes Medical Institute, Department of Pharmacology, University of California, San Francisco, 94143, USA
    Cell 90:207-16. 1997
    ..Thus, kinesin and myosin have developed distinct polymer-binding domains in a similar region with respect to their common catalytic cores...
  21. pmc Identification of a kinesin-like microtubule-based motor protein in Dictyostelium discoideum
    G McCaffrey
    Department of Pharmacology, University of California, San Francisco 94143
    EMBO J 8:3229-34. 1989
    ..Thus, an AMP-PNP-induced rigor binding may not be a characteristic of kinesins from lower eukaryotes...
  22. ncbi request reprint Microtubule disassembly by ATP-dependent oligomerization of the AAA enzyme katanin
    J J Hartman
    The Howard Hughes Medical Institute and the Department of Cellular and Molecular Pharmacology, University of California, San Francisco, CA 94143, USA
    Science 286:782-5. 1999
    ..Coupling a nucleotide-dependent oligomerization cycle to the disassembly of a target protein complex may be a general feature of ATP-hydrolyzing AAA domains...
  23. pmc Widespread cytoplasmic mRNA transport in yeast: identification of 22 bud-localized transcripts using DNA microarray analysis
    K A Shepard
    Department of Cellular and Molecular Pharmacology and Biochemistry and Biophysics and Howard Hughes Medical Institute, University of California, San Francisco, CA 94107, USA
    Proc Natl Acad Sci U S A 100:11429-34. 2003
    ..In contrast to findings in metazoans, the untranslated regions are dispensable for mRNA localization in yeast. This study reveals an unanticipated widespread use of RNA transport in budding yeast...
  24. pmc Mechanisms for focusing mitotic spindle poles by minus end-directed motor proteins
    Gohta Goshima
    The Howard Hughes Medical Institute and the Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94107, USA
    J Cell Biol 171:229-40. 2005
    ..From these results and simulations, we propose a model on how two minus end-directed motors cooperate to ensure spindle pole coalescence during mitosis...
  25. pmc Structural basis of microtubule plus end tracking by XMAP215, CLIP-170, and EB1
    Kevin C Slep
    Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, CA 94158, USA
    Mol Cell 27:976-91. 2007
    ..We propose that +TIPs, although diverse in structure, share a common property of multimerizing tubulin, thus acting as polymerization chaperones that aid in subunit addition to the microtubule plus end...
  26. pmc Controlling kinesin by reversible disulfide cross-linking. Identifying the motility-producing conformational change
    M Tomishige
    The Howard Hughes Medical Institute and the Department of Cellular and Molecular Pharmacology, University of California, San Francisco, California 94143, USA
    J Cell Biol 151:1081-92. 2000
    ..These studies indicate that conformational changes in the neck linker, not in the neck coiled-coil, drive processive movement by the kinesin motor...
  27. pmc Cell cycle-dependent dynamics and regulation of mitotic kinesins in Drosophila S2 cells
    Gohta Goshima
    The Howard Hughes Medical Institute and the Department of Cellular and Molecular Pharmacology, University of California San Francisco, San Francisco, CA 94107, USA
    Mol Biol Cell 16:3896-907. 2005
    ..These results reveal a diverse spectrum of regulatory mechanisms for controlling the localization and function of five mitotic kinesins at different stages of the cell cycle...
  28. pmc A lever-arm rotation drives motility of the minus-end-directed kinesin Ncd
    Nicholas F Endres
    The Howard Hughes Medical Institute, and the Department of Cellular and Molecular Pharmacology, University of California San Francisco, 600 16th Street, San Francisco, California 94107, USA
    Nature 439:875-8. 2006
    ..These results show that the force-producing conformational change in Ncd occurs on ATP binding, as in other kinesins, but involves the swing of a lever-arm mechanical element similar to that described for myosins...
  29. pmc Structure and functional role of dynein's microtubule-binding domain
    Andrew P Carter
    Howard Hughes Medical Institute and Department of Cellular and Molecular Pharmacology, University of California, San Francisco, CA 94158, USA
    Science 322:1691-5. 2008
    ..Surprisingly, functional data suggest that the MTBD, and not the ATPase domain, is the main determinant of the direction of dynein motility...
  30. pmc Patronin regulates the microtubule network by protecting microtubule minus ends
    Sarah S Goodwin
    The Howard Hughes Medical Institute and Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94158 2200, USA
    Cell 143:263-74. 2010
    ..We propose that Patronin caps and stabilizes microtubule minus ends, an activity that serves a critical role in the organization of the microtubule cytoskeleton...
  31. pmc The load dependence of kinesin's mechanical cycle
    C M Coppin
    Howard Hughes Medical Institute, University of California, San Francisco, CA 94143, USA
    Proc Natl Acad Sci U S A 94:8539-44. 1997
    ....
  32. ncbi request reprint Conversion of Unc104/KIF1A kinesin into a processive motor after dimerization
    Michio Tomishige
    The Howard Hughes Medical Institute and the Department of Cellular and Molecular Pharmacology, University of California, San Francisco, CA 94143, USA
    Science 297:2263-7. 2002
    ..These results suggest that Unc104/KIF1A operates in vivo by a mechanism similar to conventional kinesin and that regulation of motor dimerization may be used to control transport by this class of kinesins...
  33. pmc Genes required for mitotic spindle assembly in Drosophila S2 cells
    Gohta Goshima
    Howard Hughes Medical Institute and the Department of Cellular and Molecular Pharmacology, University of California, San Francisco, 600 16th Street, San Francisco, CA 94158, USA
    Science 316:417-21. 2007
    ..The screen, in combination with a variety of secondary assays, led to new insights into how spindle microtubules are generated; how centrosomes are positioned; and how centrioles, centrosomes, and kinetochores are assembled...
  34. pmc Communication between the AAA+ ring and microtubule-binding domain of dynein
    Andrew P Carter
    Howard Hughes Medical Institute and Department of Cellular and Molecular Pharmacology, University of California, San Francisco, CA 94158, USA
    Biochem Cell Biol 88:15-21. 2010
    ..A recent crystal structure of dynein's MTBD sheds new light on how this long-range communication along a coiled coil might occur...
  35. pmc Regulatory ATPase sites of cytoplasmic dynein affect processivity and force generation
    Carol Cho
    Howard Hughes Medical Institute, University of California, San Francisco, California 94158 2517, USA
    J Biol Chem 283:25839-45. 2008
    ..These results indicate that the nucleotide binding state at AAA3 and AAA4 can allosterically modulate microtubule binding affinity and affect dynein processivity and force production...
  36. pmc Microscopes for fluorimeters: the era of single molecule measurements
    Ronald D Vale
    Department of Cellular and Molecular Pharmacology and The Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, CA 94143, USA
    Cell 135:779-85. 2008
    ..This Essay highlights applications and challenges of single molecule studies in structural biology, cell biology, and biotechnology...
  37. pmc Myosin V motor proteins: marching stepwise towards a mechanism
    Ronald D Vale
    Department of Cellular and Molecular Pharmacology and The Howard Hughes Medical Institute, University of California, San Francisco, CA 94107, USA
    J Cell Biol 163:445-50. 2003
    ....
  38. pmc The roles of microtubule-based motor proteins in mitosis: comprehensive RNAi analysis in the Drosophila S2 cell line
    Gohta Goshima
    Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94107, USA
    J Cell Biol 162:1003-16. 2003
    ..From our phenotypic data, we construct a model for the distinct roles of molecular motors during mitosis in a single metazoan cell type...
  39. pmc It's a wonderful life: a career as an academic scientist
    Ronald D Vale
    Department of Cellular and Molecular Pharmacology, Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, CA 94158, USA
    Mol Biol Cell 21:11-4. 2010
    ..In this essay, I further elaborate by listing my top ten reasons why an academic job is a desirable career for young people who are interested in the life sciences...
  40. pmc The biological sciences in India: aiming high for the future
    Ronald D Vale
    The Howard Hughes Medical Institute and Department of Cellular and Molecular Pharmacology, University of California, San Francisco, USA
    J Cell Biol 184:342-53. 2009
    ..Such challenges are faced by the US/Europe, but are particularly acute in developing countries that are racing to achieve scientific excellence, perhaps faster than their present educational and faculty support systems will allow...
  41. pmc Role of phosphatidylinositol(4,5)bisphosphate organization in membrane transport by the Unc104 kinesin motor
    Dieter R Klopfenstein
    Department of Cellular and Molecular Pharmacology, University of California, San Francisco, 513 Parnassus Avenue, 94143, USA
    Cell 109:347-58. 2002
    ..These studies suggest that clustering of Unc104 in PtdIns(4,5)P2-containing rafts provides a trigger for membrane transport...
  42. pmc A tribute to Shinya Inoue and innovation in light microscopy
    Karen R Dell
    The Journal of Cell Biology
    J Cell Biol 165:21-5. 2004
    ....
  43. ncbi request reprint Closing of the nucleotide pocket of kinesin-family motors upon binding to microtubules
    Nariman Naber
    Department of Biochemistry, University of California, San Francisco, CA 94143, USA
    Science 300:798-801. 2003
    ..The switch movement primes the motor both for the free energy-yielding nucleotide hydrolysis reaction and for subsequent conformational changes that are crucial for the generation of force and directed motion along the microtubule...
  44. ncbi request reprint Actin-dependent localization of an RNA encoding a cell-fate determinant in yeast
    P A Takizawa
    Howard Hughes Medical Institute, Department of Cellular and Molecular Pharmacology, University of California, San Francisco 94143 0450, USA
    Nature 389:90-3. 1997
    ..Thus, as in higher eukaryotes, Saccharomyces cerevisiae employs RNA localization to generate an asymmetric distribution of proteins and hence to determine cell fate...
  45. ncbi request reprint Identification of katanin, an ATPase that severs and disassembles stable microtubules
    F J McNally
    Department of Pharmacology, University of California, San Francisco 94143
    Cell 75:419-29. 1993
    ..Katanin represents a novel type of enzyme that utilizes energy from nucleotide hydrolysis to break tubulin-tubulin bonds within a microtubule polymer, a process that may aid in disassembling complex microtubule arrays within cells...
  46. ncbi request reprint Inhibitors of kinesin activity from structure-based computer screening
    S C Hopkins
    Department of Pharmaceutical Chemistry, Howard Hughes Medical Institute, University of California, San Francisco, California 94143 0446, USA
    Biochemistry 39:2805-14. 2000
    ..These results confirm this region's role in microtubule binding and identify this pocket as a novel binding site for kinesin inhibition...
  47. pmc Structural basis of microtubule severing by the hereditary spastic paraplegia protein spastin
    Antonina Roll-Mecak
    Howard Hughes Medical Institute and Department of Cellular and Molecular Pharmacology, University of California, San Francisco, 600 16th Street, San Francisco, California 94158, USA
    Nature 451:363-7. 2008
    ..Our work also provides insights into the structural defects in spastin that arise from mutations identified in hereditary spastic paraplegia patients...
  48. pmc Intramolecular strain coordinates kinesin stepping behavior along microtubules
    Ahmet Yildiz
    Department of Cellular and Molecular Pharmacology and The Howard Hughes Medical Institute, University of California, San Francisco, CA 94158, USA
    Cell 134:1030-41. 2008
    ..Our results indicate that the kinesin motor domain senses and responds to strain in a manner that facilitates its plus-end-directed stepping and communication between its two motor domains...
  49. pmc Autoinhibition regulates the motility of the C. elegans intraflagellar transport motor OSM-3
    Miki Imanishi
    Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, CA 94107, USA
    J Cell Biol 174:931-7. 2006
    ..Interestingly, the G444E allele in C. elegans produces similar ciliary defects to an osm-3-null mutation, suggesting that autoinhibition is important for OSM-3's biological function...
  50. pmc Single-molecule analysis of dynein processivity and stepping behavior
    Samara L Reck-Peterson
    Department of Cellular and Molecular Pharmacology, University of California, San Francisco, CA 94158, USA
    Cell 126:335-48. 2006
    ....
  51. pmc Walking the walk: how kinesin and dynein coordinate their steps
    Arne Gennerich
    Howard Hughes Medical Institute and Department of Cellular and Molecular Pharmacology, University of California San Francisco, San Francisco, CA 94158 2200, USA
    Curr Opin Cell Biol 21:59-67. 2009
    ..In this review, we discuss how these processive motors coordinate the activities of their two identical motor domains so that they can walk along microtubules...
  52. pmc Force-induced bidirectional stepping of cytoplasmic dynein
    Arne Gennerich
    The Howard Hughes Medical Institute and the Department of Cellular and Molecular Pharmacology, University of California San Francisco, San Francisco, CA 94158 2200, USA
    Cell 131:952-65. 2007
    ..These results suggest a model for how dynein's two motor domains coordinate their activities during normal processive motility and provide new clues for understanding dynein-based motility in living cells...
  53. ncbi request reprint Drosophila pod-1 crosslinks both actin and microtubules and controls the targeting of axons
    Michael E Rothenberg
    Department of Physiology, Howard Hughes Medical Institute, University of California, San Francisco, 533 Parnassus Avenue, San Francisco, CA 94143, USA
    Neuron 39:779-91. 2003
    ..Taken together, these results reveal novel activities for pod-1 and show that proper levels of Dpod1, an actin/MT crosslinker, must be maintained in the growth cone for correct axon guidance...
  54. pmc Molecular dissection of the roles of nucleotide binding and hydrolysis in dynein's AAA domains in Saccharomyces cerevisiae
    Samara L Reck-Peterson
    Howard Hughes Medical Institute and Department of Cellular and Molecular Pharmacology, University of California San Francisco, San Francisco, CA 94107, USA
    Proc Natl Acad Sci U S A 101:1491-5. 2004
    ..These results show that the four conserved dynein AAA domains have distinct functions in dynein's mechanochemical cycle...
  55. ncbi request reprint The Drosophila homologue of the hereditary spastic paraplegia protein, spastin, severs and disassembles microtubules
    Antonina Roll-Mecak
    The Howard Hughes Medical Institute and the Department of Cellular and Molecular Pharmacology, University of California, San Francisco, California 94143, USA
    Curr Biol 15:650-5. 2005
    ..We show that D-spastin, like katanin, displays ATPase activity and uses energy from ATP hydrolysis to sever and disassemble microtubules; disease mutations abolish or partially interfere with these activities...
  56. pmc The lipid binding pleckstrin homology domain in UNC-104 kinesin is necessary for synaptic vesicle transport in Caenorhabditis elegans
    Dieter R Klopfenstein
    Department of Cellular and Molecular Pharmacology, University of California San Francisco, San Francisco, California 94143, USA
    Mol Biol Cell 15:3729-39. 2004
    ..These results reveal a critical role for PI(4,5)P(2) binding in UNC-104-mediated axonal transport and shows that the cargo-binding properties of the distal PH domain can affect motor output...
  57. pmc Spindly, a novel protein essential for silencing the spindle assembly checkpoint, recruits dynein to the kinetochore
    Eric R Griffis
    Howard Hughes Medical Institute, University of California, San Francisco, CA 94158, USA
    J Cell Biol 177:1005-15. 2007
    ..However, dynein's nonkinetochore functions are unaffected by Spindly depletion. Our findings indicate that Spindly is a novel regulator of mitotic dynein, functioning specifically to target dynein to kinetochores...
  58. ncbi request reprint Drosophila RhoGEF2 associates with microtubule plus ends in an EB1-dependent manner
    Stephen L Rogers
    Howard Hughes Medical Institute and Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94107, USA
    Curr Biol 14:1827-33. 2004
    ....
  59. pmc Mechanism of transport of IFT particles in C. elegans cilia by the concerted action of kinesin-II and OSM-3 motors
    Xiaoyu Pan
    Section of Molecular and Cellular Biology, Center for Genetics and Development, University of California, Davis, Davis, CA 95616, USA
    J Cell Biol 174:1035-45. 2006
    ....
  60. pmc Structural determinants for EB1-mediated recruitment of APC and spectraplakins to the microtubule plus end
    Kevin C Slep
    The Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94107, USA
    J Cell Biol 168:587-98. 2005
    ..These results provide a structural understanding of how EB1 binds two regulators of microtubule-based cell polarity...
  61. pmc Drosophila EB1 is important for proper assembly, dynamics, and positioning of the mitotic spindle
    Stephen L Rogers
    The Howard Hughes Medical Institute and Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94143, USA
    J Cell Biol 158:873-84. 2002
    ..Our results reveal crucial roles for EB1 in mitosis, which we postulate involves its ability to promote the growth and interactions of microtubules within the central spindle and at the cell cortex...
  62. pmc The affinity of the dynein microtubule-binding domain is modulated by the conformation of its coiled-coil stalk
    I R Gibbons
    Molecular and Cell Biology Department, University of California, Berkeley, California 94720, USA
    J Biol Chem 280:23960-5. 2005
    ....
  63. pmc Cloning and expression of a human kinesin heavy chain gene: interaction of the COOH-terminal domain with cytoplasmic microtubules in transfected CV-1 cells
    F Navone
    Department of Pharmacology, University of California, San Francisco 94143
    J Cell Biol 117:1263-75. 1992
    ..The finding that both the kinesin motor and tail domains can interact with cytoplasmic microtubules raises the possibility that kinesin could crossbridge and induce sliding between microtubules under certain circumstances...
  64. pmc Functional genomic screen reveals genes involved in lipid-droplet formation and utilization
    Yi Guo
    Department of Biochemistry and Biophysics, University of California, San Francisco, California 94158, USA
    Nature 453:657-61. 2008
    ..These phenotypes are conserved in mammalian cells, suggesting that insights from these studies are likely to be central to our understanding of human diseases involving excessive lipid storage...
  65. pmc Regulation of the processivity and intracellular localization of Saccharomyces cerevisiae dynein by dynactin
    Julia R Kardon
    Department of Cellular and Molecular Pharmacology and Howard Hughes Medical Institute, University of California, San Francisco, CA 94158, USA
    Proc Natl Acad Sci U S A 106:5669-74. 2009
    ..Instead, a segment of the coiled-coil of Nip100 is required for these activities. Our results directly demonstrate that dynactin increases the processivity of dynein through a mechanism independent of microtubule tethering...
  66. pmc Making more microtubules by severing: a common theme of noncentrosomal microtubule arrays?
    Antonina Roll-Mecak
    Howard Hughes Medical Institute and Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94158, USA
    J Cell Biol 175:849-51. 2006
    ..Together, these studies hint at a wider role for microtubule-severing enzymes in the formation and organization of noncentrosomal microtubule arrays by generating new seeds for microtubule growth...
  67. ncbi request reprint Making microtubules and mitotic spindles in cells without functional centrosomes
    Nicole M Mahoney
    The Howard Hughes Medical Institute and Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, California 94107, USA
    Curr Biol 16:564-9. 2006
    ..We propose that the bipolar spindle propagates its own architecture by stimulating microtubule growth, thereby augmenting the well-described microtubule nucleation pathways that take place at centrosomes and chromosomes...
  68. ncbi request reprint Kinesin motors and microtubule-based organelle transport in Dictyostelium discoideum
    Dieter R Klopfenstein
    Department of Cellular and Molecular Pharmacology, University of California San Francisco, 600 16th Street, San Francisco, CA 94107, USA
    J Muscle Res Cell Motil 23:631-8. 2002
    ..We describe how the biological functions of these motors has been dissected through a combination of biochemical to genetic approaches...
  69. pmc Polarized myosin produces unequal-size daughters during asymmetric cell division
    Guangshuo Ou
    The Howard Hughes Medical Institute and the Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94158, USA
    Science 330:677-80. 2010
    ..Thus, the balance of myosin activity on the two sides of a dividing cell can govern the size and fate of the daughters...
  70. pmc Molecular signatures of cell migration in C. elegans Q neuroblasts
    Guangshuo Ou
    The Howard Hughes Medical Institute, University of California San Francisco, San Francisco, CA 94158, USA
    J Cell Biol 185:77-85. 2009
    ..Thus, MIG-2 and INA-1 function distinctly to control Q neuroblast migration in living C. elegans...
  71. pmc Single-molecule microscopy reveals plasma membrane microdomains created by protein-protein networks that exclude or trap signaling molecules in T cells
    Adam D Douglass
    The Howard Hughes Medical Institute and Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, California 94107, USA
    Cell 121:937-50. 2005
    ..Our data suggest that diffusional trapping through protein-protein interactions creates microdomains that concentrate or exclude cell surface proteins to facilitate T cell signaling...
  72. ncbi request reprint Single-molecule imaging of fluorescent proteins
    Adam D Douglass
    Department of Cellular and Molecular Pharmacology, University of California, The Howard Hughes Medical Institute, San Francisco, California 94107, USA
    Methods Cell Biol 85:113-25. 2008
    ..Last, we discuss some common pitfalls involved in analyzing single molecule datasets, and consider some of the unique information that can be obtained using these techniques...
  73. pmc Mechanisms for segregating T cell receptor and adhesion molecules during immunological synapse formation in Jurkat T cells
    Yoshihisa Kaizuka
    The Howard Hughes Medical Institute and the Department of Cellular and Molecular Pharmacology, University of California, San Francisco, CA 94158, USA
    Proc Natl Acad Sci U S A 104:20296-301. 2007
    ..Our results reveal that TCR and adhesion molecules spatially partition from one another well before the formation of a mature IS and that differential actin interactions help to shape and maintain the final bull's-eye pattern of the IS...
  74. pmc Unbiased selection of localization elements reveals cis-acting determinants of mRNA bud localization in Saccharomyces cerevisiae
    Ashwini Jambhekar
    Department of Biochemistry and Biophysics, University of California, San Francisco, 94107, USA
    Proc Natl Acad Sci U S A 102:18005-10. 2005
    ..Our findings further our understanding of RNA recognition by the She complex, and the methods used here should be applicable for elucidating minimal RNA motifs involved in many other types of interactions...
  75. pmc A whole genome RNAi screen of Drosophila S2 cell spreading performed using automated computational image analysis
    Michael V D'Ambrosio
    The Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, CA 94158, USA
    J Cell Biol 191:471-8. 2010
    ....
  76. pmc Mechanism of prion propagation: amyloid growth occurs by monomer addition
    Sean R Collins
    Howard Hughes Medical Institute, Department of Cellular and Molecular Pharmacology, University of California, San Francisco, USA
    PLoS Biol 2:e321. 2004
    ..Thus, amyloid growth can occur by monomer addition in a reaction distinct from and competitive with formation of potentially toxic oligomeric intermediates...
  77. ncbi request reprint How kinesin waits between steps
    Teppei Mori
    Department of Applied Physics, The University of Tokyo, Tokyo 113 8656, Japan
    Nature 450:750-4. 2007
    ..On the basis of these results, we suggest a model for how transitions in the ATPase cycle position the two kinesin heads and drive their hand-over-hand motion...
  78. ncbi request reprint Distinct mechanisms govern the localisation of Drosophila CLIP-190 to unattached kinetochores and microtubule plus-ends
    Nikola S Dzhindzhev
    The Wellcome Trust Centre for Cell Biology, Institute of Cell Biology, School of Biological Sciences, The University of Edinburgh, Edinburgh, EH9 3JR, UK
    J Cell Sci 118:3781-90. 2005
    ..These results indicate distinct molecular requirements for CLIP-190 localisation to unattached kinetochores in mitosis and microtubule ends in interphase...
  79. ncbi request reprint Functionally distinct kinesin-13 family members cooperate to regulate microtubule dynamics during interphase
    Vito Mennella
    Department of Physiology and Biophysics, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Nat Cell Biol 7:235-45. 2005
    ..Our data also suggest that KLP10A is deposited on microtubules by the plus-end tracking protein, EB1. Our findings support a model in which these two members of the kinesin-13 family divide the labour of microtubule depolymerization...
  80. pmc Augmin: a protein complex required for centrosome-independent microtubule generation within the spindle
    Gohta Goshima
    Institute for Advanced Research, Nagoya University, Chikusa ku, Nagoya 464 8601, Japan
    J Cell Biol 181:421-9. 2008
    ..Our results suggest that an important mitotic function for gamma-tubulin may lie within the spindle, where augmin and gamma-tubulin function cooperatively to amplify the number of microtubules...
  81. pmc Distinct conformations of the kinesin Unc104 neck regulate a monomer to dimer motor transition
    Jawdat Al-Bassam
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    J Cell Biol 163:743-53. 2003
    ..We suggest that the Unc104 neck regulates motility by switching from a self-folded, repressed state to a dimerized conformation that can support fast processive movement...
  82. ncbi request reprint Two mitotic kinesins cooperate to drive sister chromatid separation during anaphase
    Gregory C Rogers
    Department of Physiology and Biophysics, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Nature 427:364-70. 2004
    ..The other, KLP10A, is required to depolymerize microtubules at their pole-associated minus ends, thereby moving chromatids by means of poleward flux...
  83. ncbi request reprint Kinesin walks hand-over-hand
    Ahmet Yildiz
    Center for Biophysics and Computational Biology, University of Illinois, Urbana Champaign, IL 61801, USA
    Science 303:676-8. 2004
    ..These results strongly support a hand-over-hand mechanism, and not an inchworm mechanism. In addition, our results suggest that kinesin is bound by both heads to the microtubule while it waits for adenosine triphosphate in between steps...
  84. ncbi request reprint Single-molecule observations of neck linker conformational changes in the kinesin motor protein
    Michio Tomishige
    Department of Applied Physics, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 8656, Japan
    Nat Struct Mol Biol 13:887-94. 2006
    ..During ATP-driven motility, the neck linkers switch between these conformational states. These results support the notion that neck linker movements accompany the 'hand-over-hand' motion of the two motor domains...
  85. ncbi request reprint Length control of the metaphase spindle
    Gohta Goshima
    Physiology Course 2004, Marine Biological Laboratory, Woods Hole, Massachusetts 02543, USA
    Curr Biol 15:1979-88. 2005
    ..Although a number of molecular perturbations have been shown to influence spindle length, a global understanding of the factors that determine metaphase spindle length has not been achieved...
  86. pmc The Khd1 protein, which has three KH RNA-binding motifs, is required for proper localization of ASH1 mRNA in yeast
    Kenji Irie
    Department of Molecular Biology, Graduate School of Science, Nagoya University, Chikusa ku, Nagoya 464 8602, Japan
    EMBO J 21:1158-67. 2002
    ..These results suggest that Khd1 may function in the linkage between ASH1 mRNA localization and its translation...
  87. pmc Distinct pathways control recruitment and maintenance of myosin II at the cleavage furrow during cytokinesis
    Sara O Dean
    Department of Biochemistry, Stanford University, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 102:13473-8. 2005
    ....
  88. pmc A standardized kinesin nomenclature
    Carolyn J Lawrence
    Department of Plant Biology, The University of Georgia, Athens, GA 30602, USA
    J Cell Biol 167:19-22. 2004
    ..The scheme unifies all previous phylogenies and nomenclature proposals, while allowing individual sequence names to remain the same, and for expansion to occur as new sequences are discovered...