George J Turner
Affiliation: University of Miami
- Structural basis of perturbed pKa values of catalytic groups in enzyme active sitesThomas K Harris
Department of Biochemistry and Molecular Biology, University of Miami School of Medicine, Florida 33101 6129, USA
IUBMB Life 53:85-98. 2002..The magnitudes of these pKa perturbations are analyzed with respect to the structural details of the active-site microenvironment and the energetics of the reactions that they catalyze...
- High-throughput screening of bacteriorhodopsin mutants in whole cell pastesLynell C Martinez
Department of Physiology and Biophysics and the Neurosciences Program, The University of Miami School of Medicine, Miami, FL 33101, USA
Biochim Biophys Acta 1564:91-8. 2002..We demonstrate that whole cell reflectance spectroscopy can be used to efficiently characterize the large numbers of mutants generated by engineering strategies that exploit saturation mutagenesis...
- Subdomains in the F and G helices of bacteriorhodopsin regulate the conformational transitions of the reprotonation mechanismLynell C Martinez
Department of Physiology and Biophysics and the Neurosciences Program, University of Miami School of Medicine, Miami, Florida 33101, USA
Proteins 48:269-82. 2002....
- G-protein-coupled receptor domain overexpression in Halobacterium salinarum: long-range transmembrane interactions in heptahelical membrane proteinsVeli Pekka Jaakola
Structural Biology and Biophysics, Institute of Biotechnology, University of Helsinki, Helsinki, Finland
Proteins 60:412-23. 2005..g., signals) across the cellular membrane. This gene fusion approach may have general applicability for physical studies of G-protein-coupled receptor domains in the context of the bR structural scaffold...