Roger Tsien

Summary

Affiliation: University of California
Country: USA

Publications

  1. ncbi Multicolor and electron microscopic imaging of connexin trafficking
    Guido Gaietta
    National Center for Microscopy and Imaging Research, Department of Neurosciences, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA
    Science 296:503-7. 2002
  2. ncbi Genetically targeted chromophore-assisted light inactivation
    Oded Tour
    Howard Hughes Medical Institute, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093 0647, USA
    Nat Biotechnol 21:1505-8. 2003
  3. pmc A drug-controllable tag for visualizing newly synthesized proteins in cells and whole animals
    Michael Z Lin
    Department of Pharmacology and Howard Hughes Medical Institute, University of California at San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0647, USA
    Proc Natl Acad Sci U S A 105:7744-9. 2008
  4. pmc Fluorescent ligand for human progesterone receptor imaging in live cells
    Roy Weinstain
    Department of Pharmacology 0647, University of California, San Diego, La Jolla, CA 92093, USA
    Bioconjug Chem 24:766-71. 2013
  5. pmc In vivo targeting of hydrogen peroxide by activatable cell-penetrating peptides
    Roy Weinstain
    Department of Pharmacology, Department of Chemistry and Biochemistry, and Howard Hughes Medical Institute, University of California San Diego, La Jolla, California 92093, United States
    J Am Chem Soc 136:874-7. 2014
  6. pmc Fluorescent and photo-oxidizing TimeSTAMP tags track protein fates in light and electron microscopy
    Margaret T Butko
    Department of Pharmacology and Howard Hughes Medical Institute, University of California San Diego, La Jolla, California, USA
    Nat Neurosci 15:1742-51. 2012
  7. pmc Ratiometric activatable cell-penetrating peptides provide rapid in vivo readout of thrombin activation
    Michael Whitney
    Department of Pharmacology, UCSD School of Medicine, University of California San Diego, La Jolla, USA
    Angew Chem Int Ed Engl 52:325-30. 2013
  8. pmc A genetically encoded tag for correlated light and electron microscopy of intact cells, tissues, and organisms
    Xiaokun Shu
    Howard Hughes Medical Institute, University of California at San Diego, La Jolla, California, United States of America
    PLoS Biol 9:e1001041. 2011
  9. pmc Parallel in vivo and in vitro selection using phage display identifies protease-dependent tumor-targeting peptides
    Mike Whitney
    Department of Pharmacology, University of California at San Diego, La Jolla, California 92093, USA
    J Biol Chem 285:22532-41. 2010
  10. pmc Detection and monitoring of localized matrix metalloproteinase upregulation in a murine model of asthma
    Csilla N Felsen
    Howard Hughes Medical Institute, Univ California San Diego, 9500 Gilman Dr, George Palade 310, La Jolla, CA 92093 0647
    Am J Physiol Lung Cell Mol Physiol 306:L764-74. 2014

Research Grants

Collaborators

Detail Information

Publications72

  1. ncbi Multicolor and electron microscopic imaging of connexin trafficking
    Guido Gaietta
    National Center for Microscopy and Imaging Research, Department of Neurosciences, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA
    Science 296:503-7. 2002
    ..Selective imaging by correlated optical and electron microscopy of protein molecules of known ages will clarify fundamental processes of protein trafficking in situ...
  2. ncbi Genetically targeted chromophore-assisted light inactivation
    Oded Tour
    Howard Hughes Medical Institute, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093 0647, USA
    Nat Biotechnol 21:1505-8. 2003
    ..ReAsH-mediated CALI acts largely via singlet oxygen because quenchers or enhancers of singlet oxygen respectively inhibit or enhance CALI...
  3. pmc A drug-controllable tag for visualizing newly synthesized proteins in cells and whole animals
    Michael Z Lin
    Department of Pharmacology and Howard Hughes Medical Institute, University of California at San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0647, USA
    Proc Natl Acad Sci U S A 105:7744-9. 2008
    ..We demonstrate applications of TimeSTAMP in retrospectively identifying growing synapses in cultured neurons and in visualizing the distribution of recently synthesized proteins in intact fly brains...
  4. pmc Fluorescent ligand for human progesterone receptor imaging in live cells
    Roy Weinstain
    Department of Pharmacology 0647, University of California, San Diego, La Jolla, CA 92093, USA
    Bioconjug Chem 24:766-71. 2013
    ..The design and properties of the BODIPY-labeled RU486 make it a potential candidate for in vivo imaging of PR by positron emission tomography through incorporation of (18)F into the BODIPY core...
  5. pmc In vivo targeting of hydrogen peroxide by activatable cell-penetrating peptides
    Roy Weinstain
    Department of Pharmacology, Department of Chemistry and Biochemistry, and Howard Hughes Medical Institute, University of California San Diego, La Jolla, California 92093, United States
    J Am Chem Soc 136:874-7. 2014
    ..Targeting H2O2 has potential applications in imaging and therapy of diseases related to oxidative stress. ..
  6. pmc Fluorescent and photo-oxidizing TimeSTAMP tags track protein fates in light and electron microscopy
    Margaret T Butko
    Department of Pharmacology and Howard Hughes Medical Institute, University of California San Diego, La Jolla, California, USA
    Nat Neurosci 15:1742-51. 2012
    ..These results demonstrate the versatility of the TimeSTAMP approach for visualizing newly synthesized proteins in neurons...
  7. pmc Ratiometric activatable cell-penetrating peptides provide rapid in vivo readout of thrombin activation
    Michael Whitney
    Department of Pharmacology, UCSD School of Medicine, University of California San Diego, La Jolla, USA
    Angew Chem Int Ed Engl 52:325-30. 2013
    ..The most advanced RACPP uses norleucine (Nle)-TPRSFL as a linker that increases sensitivity to thrombin by about 90-fold...
  8. pmc A genetically encoded tag for correlated light and electron microscopy of intact cells, tissues, and organisms
    Xiaokun Shu
    Howard Hughes Medical Institute, University of California at San Diego, La Jolla, California, United States of America
    PLoS Biol 9:e1001041. 2011
    ..Thus SynCAM1 and SynCAM2 could be heterophilic partners. MiniSOG may do for EM what Green Fluorescent Protein did for fluorescence microscopy...
  9. pmc Parallel in vivo and in vitro selection using phage display identifies protease-dependent tumor-targeting peptides
    Mike Whitney
    Department of Pharmacology, University of California at San Diego, La Jolla, California 92093, USA
    J Biol Chem 285:22532-41. 2010
    ..The identification of an ACPP that targets tumor expressed proteases without rational design highlights the value of unbiased selection schemes for the development of potential therapeutic agents...
  10. pmc Detection and monitoring of localized matrix metalloproteinase upregulation in a murine model of asthma
    Csilla N Felsen
    Howard Hughes Medical Institute, Univ California San Diego, 9500 Gilman Dr, George Palade 310, La Jolla, CA 92093 0647
    Am J Physiol Lung Cell Mol Physiol 306:L764-74. 2014
    ....
  11. pmc Very long-term memories may be stored in the pattern of holes in the perineuronal net
    Roger Y Tsien
    Department of Pharmacology, and Howard Hughes Medical Institute, University of California at San Diego, La Jolla, CA 92093 0647, USA
    Proc Natl Acad Sci U S A 110:12456-61. 2013
    ..The snapshot reporter should enable more precise inhibition or potentiation of PNN erosion to compare with behavioral consequences. Finally, biosynthesis of PNN components and proteases would be imaged. ..
  12. ncbi Building and breeding molecules to spy on cells and tumors
    Roger Y Tsien
    Department of Pharmacology, University of California, San Diego, Howard Hughes Medical Institute, 310 George Palade Laboratories 0647, La Jolla, CA 92093 0647, USA
    FEBS Lett 579:927-32. 2005
    ....
  13. ncbi Imagining imaging's future
    Roger Y Tsien
    Howard Hughes Medical Institute, Department of Pharmacology, University of California, San Diego, La Jolla 92093 0647, USA
    Nat Rev Mol Cell Biol . 2003
    ..This quest will require input from physics, chemistry, and the genetics and biochemistry of diverse organisms with useful talents...
  14. ncbi Breeding and building molecules to spy on cells and tumors
    Roger Y Tsien
    Howard Hughes Medical Institute, University of California, San Diego, CA, USA
    Keio J Med 55:127-40. 2006
  15. ncbi Reducing the environmental sensitivity of yellow fluorescent protein. Mechanism and applications
    O Griesbeck
    Howard Hughes Medical Institute, University of California, San Diego, La Jolla, California 92093-0647, USA
    J Biol Chem 276:29188-94. 2001
    ..Citrine is superior to all previous YFPs except when pH or halide sensitivity is desired and is particularly advantageous within genetically encoded fluorescent indicators of physiological signals...
  16. pmc Overexpression of CALNUC (nucleobindin) increases agonist and thapsigargin releasable Ca2+ storage in the Golgi
    P Lin
    Division of Cellular and Molecular Medicine, University of California San Diego, La Jolla, California 92093 0651, USA
    J Cell Biol 145:279-89. 1999
    ..These results provide direct evidence that CALNUC binds Ca2+ in vivo and together with SERCA and IP3R is involved in establishment of the agonist-mobilizable Golgi Ca2+ store...
  17. ncbi Mechanisms of pH regulation in the regulated secretory pathway
    M M Wu
    Department of Molecular and Cell Biology, University of California, Berkeley, California 94720-3200, USA
    J Biol Chem 276:33027-35. 2001
    ..We found that P(H+) decreased progressively from ER to Golgi to MSGs, and proper acidification of Golgi and MSGs required gradual decreases in P(H+) and successive increases in the active H(+) pump density...
  18. ncbi Fluorescent indicators for Ca2+ based on green fluorescent proteins and calmodulin
    A Miyawaki
    Department of Pharmacology, University of California, San Diego, La Jolla 92093 0647, USA
    Nature 388:882-7. 1997
    ..Thus FRET between GFP mutants can monitor localized Ca2+ signals and protein heterodimerization in individual live cells...
  19. ncbi Cytochrome c is released in a single step during apoptosis
    J C Goldstein
    La Jolla Institute for Allergy and Immunology, 10355 Science Center Dr, San Diego, CA 92121, USA
    Cell Death Differ 12:453-62. 2005
    ..c-GFP. We conclude that there is a general mechanism responsible for cytochrome c release that proceeds in a single step that is independent of changes in DeltaPsi(m)...
  20. ncbi Spatio-temporal dynamics of cyclic AMP signals in an intact neural circuitm
    C M Hempel
    Department of Biology, Howard Hughes Medical Institute, University of California, San Diego, La Jolla 92093, USA
    Nature 384:166-9. 1996
    ..Diffusion of cAMP may explain how transient localized inputs to a neuron can produce long-range effects such as long-term changes in gene expression...
  21. ncbi A new caged Ca2+, azid-1, is far more photosensitive than nitrobenzyl-based chelators
    S R Adams
    Department of Pharmacology, 310 Cellular and Molecular Medicine West, University of California San Diego, La Jolla, CA 92093 0647, USA
    Chem Biol 4:867-78. 1997
    ..As these chelators are all based on nitrobenzyl photochemistry, we explored the use of other photosensitive moieties to generate a new caged calcium with improved properties...
  22. ncbi Alteration of stimulus-specific guard cell calcium oscillations and stomatal closing in Arabidopsis det3 mutant
    G J Allen
    Cell and Developmental Biology Section, Division of Biology and Center for Molecular Genetics, University of California, San Diego, La Jolla, CA 92093 0116, USA
    Science 289:2338-42. 2000
    ..Moreover, in det3 guard cells, experimentally imposing external calcium-induced oscillations rescued stomatal closure. These data provide genetic evidence that stimulus-specific calcium oscillations are necessary for stomatal closure...
  23. ncbi Recent advances in technology for measuring and manipulating cell signals
    D A Zacharias
    Department of Pharmacology, Howard Hughes Medical Institute, University of California at San Diego, La Jolla, 0647, CA 92093 0647, USA
    Curr Opin Neurobiol 10:416-21. 2000
    ..Signal transduction research has made some glowing progress in the past 12 months. Recent advances in fluorescent proteins, small molecule fluorophores and imaging technology are generating new ways to investigate signal transduction...
  24. pmc Video-rate scanning two-photon excitation fluorescence microscopy and ratio imaging with cameleons
    G Y Fan
    National Center for Microscopy and Imaging Research, Department of Neurosciences, School of Medicine, San Diego, California 92093, USA
    Biophys J 76:2412-20. 1999
    ..Dynamic two-photon images of cardiac myocytes and ratio images of yellow cameleon-2.1, -3.1, and -3.1nu are also presented...
  25. ncbi Cameleon calcium indicator reports cytoplasmic calcium dynamics in Arabidopsis guard cells
    G J Allen
    Department of Biology, University of California, San Diego, La Jolla, CA 92093 0116, USA
    Plant J 19:735-47. 1999
    ..This technical advance in combination with cell biological and molecular genetic approaches will become an invaluable tool in the dissection of plant cell signal transduction pathways...
  26. ncbi Absence of cerebellar long-term depression in mice lacking neuronal nitric oxide synthase
    V Lev-Ram
    Department of Pharmacology, University of California San Diego, La Jolla 92093 0647, USA
    Learn Mem 4:169-77. 1997
    ..Also slices from knockout mice show no deficit in cGMP elevation in response to exogenous NO. Therefore, prolonged absence of nNOS allows atrophy of the signaling pathway downstream of cGMP...
  27. ncbi Changes in intramitochondrial and cytosolic pH: early events that modulate caspase activation during apoptosis
    S Matsuyama
    Programme on Apoptosis and Cell Death Regulation, The Burnham Institute, 10901 North Torrey Pines Road, La Jolla, California 92037, USA
    Nat Cell Biol 2:318-25. 2000
    ..These findings indicate that mitochondria-mediated alteration of intracellular pH may be an early event that regulates caspase activation in the mitochondrial pathway for apoptosis...
  28. pmc Ligand-dependent interactions of coactivators steroid receptor coactivator-1 and peroxisome proliferator-activated receptor binding protein with nuclear hormone receptors can be imaged in live cells and are required for transcription
    J Llopis
    Department of Pharmacology, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA
    Proc Natl Acad Sci U S A 97:4363-8. 2000
    ..These findings suggest that ligand-dependent transcriptional activities of the RAR and ER require concurrent or sequential recruitment of SRC-1 and PBP-containing coactivator complexes...
  29. pmc Genetically encoded fluorescent reporters of protein tyrosine kinase activities in living cells
    A Y Ting
    Department of Pharmacology, Howard Hughes Medical Institute, University of California at San Diego, La Jolla, CA 92093, USA
    Proc Natl Acad Sci U S A 98:15003-8. 2001
    ..These results establish a general strategy for nondestructively imaging dynamic protein tyrosine kinase activities with high spatial and temporal resolution in single living cells...
  30. pmc Genetically encoded reporters of protein kinase A activity reveal impact of substrate tethering
    J Zhang
    Department of Pharmacology, University of California at San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA
    Proc Natl Acad Sci U S A 98:14997-5002. 2001
    ....
  31. ncbi Isoform-specific PKA dynamics revealed by dye-triggered aggregation and DAKAP1alpha-mediated localization in living cells
    Brent R Martin
    Department of Pharmacology, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA
    Chem Biol 14:1031-42. 2007
    ..Overall, effective separation of type I PKA is substrate dependent, whereas type II PKA dissociation relies on autophosphorylation...
  32. pmc Golgi twins in late mitosis revealed by genetically encoded tags for live cell imaging and correlated electron microscopy
    Guido M Gaietta
    National Center for Microscopy and Imaging Research, Center for Research on Biological Structure, University of California at San Diego, La Jolla, CA 92093, USA
    Proc Natl Acad Sci U S A 103:17777-82. 2006
    ..Our studies provide previously undescribed insights into Golgi disassociation and reassembly during mitosis and offer a powerful approach to follow recombinant protein distribution in 4D imaging and correlated high-resolution analysis...
  33. ncbi The fluorescent toolbox for assessing protein location and function
    Ben N G Giepmans
    National Center for Microscopy and Imaging Research, Center for Research in Biological Systems, Department of Neurosciences, University of California, San Diego, La Jolla, CA 92093, USA
    Science 312:217-24. 2006
    ..Small organic fluorescent dyes, nanocrystals ("quantum dots"), autofluorescent proteins, small genetic encoded tags that can be complexed with fluorochromes, and combinations of these probes are highlighted...
  34. ncbi Dynamics of the upper 50-kDa domain of myosin V examined with fluorescence resonance energy transfer
    Mingxuan Sun
    Department of Biology, University of North Carolina, Charlotte, NC 28223, USA
    J Biol Chem 281:5711-7. 2006
    ....
  35. ncbi Mammalian cell-based optimization of the biarsenical-binding tetracysteine motif for improved fluorescence and affinity
    Brent R Martin
    Department of Pharmacology, University of California, San Diego, 9500 Gilman Dr, La Jolla, California, 92093 0647, USA
    Nat Biotechnol 23:1308-14. 2005
    ..These improved biarsenical-tetracysteine motifs should enable detection of a much broader spectrum of cellular proteins...
  36. ncbi A FlAsH-based FRET approach to determine G protein-coupled receptor activation in living cells
    Carsten Hoffmann
    Institute of Pharmacology and Toxicology, University of Wurzburg, Germany
    Nat Methods 2:171-6. 2005
    ..Thus, FRET from CFP to FlAsH reports GPCR activation in living cells without disturbing receptor function and shows that the small size of the tetracysteine-biarsenical tag can be decisively advantageous...
  37. pmc Improving the photostability of bright monomeric orange and red fluorescent proteins
    Nathan C Shaner
    Department of Pharmacology, University of California at San Diego, 9500 Gilman Drive, La Jolla, California 92093, USA
    Nat Methods 5:545-51. 2008
    ..and TagRFP (a monomeric derivative of eqFP578 from Entacmaea quadricolor) that maintain most of the beneficial qualities of the original proteins and perform as reliably as Aequorea victoria GFP derivatives in fusion constructs...
  38. ncbi Tetracysteine genetic tags complexed with biarsenical ligands as a tool for investigating gap junction structure and dynamics
    Gina E Sosinsky
    National Center for Microscopy and Imaging Research, Department of Neurosciences, University of California, San Diego, La Jolla, CA 92093, USA
    Cell Commun Adhes 10:181-6. 2003
    ....
  39. ncbi Investigating mitochondrial redox potential with redox-sensitive green fluorescent protein indicators
    George T Hanson
    Department of Biology, Institute of Molecular Biology, University of Oregon, Eugene, OR 97403 1229, USA
    J Biol Chem 279:13044-53. 2004
    ..T. Dooley, T. M. Dore, G. Hanson, W. C. Jackson, S. J. Remington, and R. Y. Tsien, submitted for publication), it is shown that the cytosol of HeLa cells is also unusually reducing but somewhat less so than the mitochondrial matrix...
  40. ncbi Activity-dependent regulation of dendritic synthesis and trafficking of AMPA receptors
    William Ju
    Nancy Friend Pritzker Laboratory, Department of Psychiatry and Behavioral Sciences, 1201 Welch Road, Stanford University School of Medicine, Palo Alto, California 94304, USA
    Nat Neurosci 7:244-53. 2004
    ....
  41. pmc A genetically encoded fluorescent reporter reveals oscillatory phosphorylation by protein kinase C
    Jonathan D Violin
    Department of Pharmacology, University of California, San Diego, La Jolla 92093, USA
    J Cell Biol 161:899-909. 2003
    ..Thus, specificity of PKC signaling depends on the local second messenger-controlled equilibrium between kinase and phosphatase activities to result in strict calcium-controlled temporal regulation of substrate phosphorylation...
  42. ncbi Detection of calcium transients in Drosophila mushroom body neurons with camgaroo reporters
    Dinghui Yu
    Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas 77030, USA
    J Neurosci 23:64-72. 2003
    ..Fluorescence was observed to increase in all three classes of mushroom body neurons, indicating that all types respond to cholinergic innervation...
  43. ncbi Creating new fluorescent probes for cell biology
    Jin Zhang
    Department of Pharmacology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, 18 496, Cambridge, Massachusetts 02139, USA
    Nat Rev Mol Cell Biol 3:906-18. 2002
    ....
  44. ncbi Imaging dynamic redox changes in mammalian cells with green fluorescent protein indicators
    Colette T Dooley
    Department of Chemistry and Biochemistry, University of California San Diego, La Jolla, California 92093 0647, USA
    J Biol Chem 279:22284-93. 2004
    ..g. epidermal growth factor and lysophosphatidic acid...
  45. ncbi New biarsenical ligands and tetracysteine motifs for protein labeling in vitro and in vivo: synthesis and biological applications
    Stephen R Adams
    Department of Pharmacology, Department of Chemistry and Biochemistry, Howard Hughes Medical Institute, and Biomedical Sciences Program, University of California, San Diego, La Jolla, California 92093 0647, USA
    J Am Chem Soc 124:6063-76. 2002
    ..Analogous biarsenicals enable affinity chromatography, fluorescence anisotropy measurements, and electron-microscopic localization of tetracysteine-tagged proteins...
  46. ncbi Partitioning of lipid-modified monomeric GFPs into membrane microdomains of live cells
    David A Zacharias
    Department of Pharmacology, Biomedical Sciences Graduate Program, and, Howard Hughes Medical Institute, University of California, San Diego, La Jolla, CA 92093 0647, USA
    Science 296:913-6. 2002
    ..Thus the nature of the lipid anchor on a protein is sufficient to determine submicroscopic localization within the plasma membrane...
  47. ncbi Exploration of new chromophore structures leads to the identification of improved blue fluorescent proteins
    Hui Wang Ai
    University of Alberta, Department of Chemistry, Edmonton, Alberta, Canada T6G 2G2
    Biochemistry 46:5904-10. 2007
    ..These new variants should greatly facilitate multicolor fluorescent imaging by legitimizing blue fluorescing proteins as practical and robust members of the fluorescent protein "toolkit"...
  48. ncbi Evolving proteins in mammalian cells using somatic hypermutation
    Lei Wang
    The Jack H Skirball Center for Chemical Biology and Proteomics, The Salk Institute for Biological Studies, 10010 N Torrey Pines Road, La Jolla, California 92037, USA
    Nat Protoc 1:1346-50. 2006
    ..For each round of evolution, it takes approximately 1 d to mutate the target gene, approximately 0.5-1 d to select or screen, and 2-4 d to propagate the cells for the next round depending on how many cells are collected...
  49. pmc Calcium Green FlAsH as a genetically targeted small-molecule calcium indicator
    Oded Tour
    Howard Hughes Medical Institute, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093 0647, USA
    Nat Chem Biol 3:423-31. 2007
    ..With high Ca(2+) buffering, CaGF signals decay as rapidly as the calcium currents, as expected for submicron Ca(2+) domains immediately surrounding active channels. Thus CaGF can report highly localized, rapid [Ca(2+)] dynamics...
  50. pmc Bcl-2-mediated alterations in endoplasmic reticulum Ca2+ analyzed with an improved genetically encoded fluorescent sensor
    Amy E Palmer
    Department of Pharmacology and Howard Hughes Medical Institute, University of California at San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0647, USA
    Proc Natl Acad Sci U S A 101:17404-9. 2004
    ....
  51. ncbi All-optical histology using ultrashort laser pulses
    Philbert S Tsai
    Department of Physics, University of California, San Diego, La Jolla, CA 92093, USA
    Neuron 39:27-41. 2003
    ..In a second example, the microvasculature within a block of neocortex was measured and reconstructed with micron resolution...
  52. pmc Optical measurement of synaptic glutamate spillover and reuptake by linker optimized glutamate-sensitive fluorescent reporters
    Samuel Andrew Hires
    Graduate Program in Neurosciences and Department of Pharmacology and Howard Hughes Medical Institute, University of California at San Diego, 9500 Gilman Drive, La Jolla, CA 92093
    Proc Natl Acad Sci U S A 105:4411-6. 2008
    ..Stimulation frequency-dependent modulation of spillover suggests a mechanism for nonsynaptic neuronal communication...
  53. pmc Tumor imaging by means of proteolytic activation of cell-penetrating peptides
    Tao Jiang
    Howard Hughes Medical Institute and Department of Pharmacology, University of California at San Diego, La Jolla, CA 92093 0647, USA
    Proc Natl Acad Sci U S A 101:17867-72. 2004
    ....
  54. pmc A monomeric red fluorescent protein
    Robert E Campbell
    Department of Pharmacology University of California at San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA
    Proc Natl Acad Sci U S A 99:7877-82. 2002
    ....
  55. pmc A new form of cerebellar long-term potentiation is postsynaptic and depends on nitric oxide but not cAMP
    Varda Lev-Ram
    Department of Pharmacology, The Howard Hughes Medical Institute, University of California at San Diego, La Jolla, CA 92093 0647, USA
    Proc Natl Acad Sci U S A 99:8389-93. 2002
    ..This LTP is postsynaptically expressed, enhanced by chelating postsynaptic Ca(2+), and depends on nitric oxide but not cAMP or cGMP, making it a plausible anti-Hebbian counterpart to Hebbian LTD...
  56. ncbi Measuring calcium signaling using genetically targetable fluorescent indicators
    Amy E Palmer
    Department of Chemistry and Biochemistry, University of Colorado, Boulder, Colorado 80309 0215, USA
    Nat Protoc 1:1057-65. 2006
    ..An experiment and calibration will probably take a few hours. Finally, the offline data workup can take approximately 1 d depending on the extent of analysis...
  57. pmc A hexahistidine-Zn2+-dye label reveals STIM1 surface exposure
    Christina T Hauser
    Department of Pharmacology, Howard Hughes Medical Institute, University of California at San Diego, La Jolla, CA 92093 0647, USA
    Proc Natl Acad Sci U S A 104:3693-7. 2007
    ..Whereas N-terminal fluorescent protein fusions interfere with surface exposure of STIM1, short His(6) tags are accessible to the dye or antibodies, demonstrating externalization...
  58. ncbi Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein
    Nathan C Shaner
    Nat Biotechnol 22:1567-72. 2004
    ..Three monomers with distinguishable hues from yellow-orange to red-orange have higher quantum efficiencies...
  59. pmc Functional fluorescent Ca2+ indicator proteins in transgenic mice under TET control
    Mazahir T Hasan
    Max Planck Institute for Medical Research, Heidelberg, Germany
    PLoS Biol 2:e163. 2004
    ....
  60. ncbi Visualizing the mechanical activation of Src
    Yingxiao Wang
    Department of Bioengineering and The Whitaker Institute of Biomedical Engineering, La Jolla, California 92093, USA
    Nature 434:1040-5. 2005
    ..We find that the transmission of mechanically induced Src activation is a dynamic process that directs signals via the cytoskeleton to spatial destinations...
  61. ncbi Breeding molecules to spy on cells
    Roger Y Tsien
    University of California, San Diego San Diego, California, USA
    Harvey Lect 99:77-93. 2003
  62. pmc Imaging tri-fusion multimodality reporter gene expression in living subjects
    Pritha Ray
    Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA 90095, USA
    Cancer Res 64:1323-30. 2004
    ..Imaging of reporter gene expression from single cells to living animals with the help of a single tri-fusion reporter gene will have the potential to accelerate translational cancer research...
  63. ncbi Insulin disrupts beta-adrenergic signalling to protein kinase A in adipocytes
    Jin Zhang
    Department of Pharmacology, University of California at San Diego, La Jolla, California 92093, USA
    Nature 437:569-73. 2005
    ..Chronically high insulin levels may disrupt the close apposition of beta-adrenergic receptors and PKA, identifying a new mechanism for crosstalk between heterologous signal transduction pathways...
  64. ncbi Novel fluorogenic substrates for imaging beta-lactamase gene expression
    Wenzhong Gao
    Department of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging, University of California, Los Angeles, California 90095 1770, USA
    J Am Chem Soc 125:11146-7. 2003
    ..These new fluorogenic substrates should find uses in clinical diagnostics and facilitate the applications of beta-lactamase as a biosensor...
  65. ncbi A guide to choosing fluorescent proteins
    Nathan C Shaner
    Department of Pharmacology, University of California at San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA
    Nat Methods 2:905-9. 2005
    ....
  66. ncbi Molecular biology and mutation of green fluorescent protein
    David A Zacharias
    The Whitney Laboratory for Marine Bioscience, University of Florida Department of Neuroscience, St Auqustine, FL, USA
    Methods Biochem Anal 47:83-120. 2006
  67. ncbi Ca2+ indicators based on computationally redesigned calmodulin-peptide pairs
    Amy E Palmer
    Department of Pharmacology and Howard Hughes Medical Institute, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093, USA
    Chem Biol 13:521-30. 2006
    ..These redesigned cameleons show significant improvements over previous versions in the ability to monitor Ca(2+) in the cytoplasm as well as distinct subcellular localizations, such as the plasma membrane of neurons and the mitochondria...
  68. ncbi Novel chromophores and buried charges control color in mFruits
    Xiaokun Shu
    Institute of Molecular Biology and Department of Physics, University of Oregon, Eugene, Oregon 97403, USA
    Biochemistry 45:9639-47. 2006
    ..pH-dependent spectral shifts of mCherry and mStrawberry appear to result from the titration of Glu 215, although, for mStrawberry, partial cyclization of Thr 66 may contribute at high pH...
  69. pmc Imaging of cAMP levels and protein kinase A activity reveals that retinal waves drive oscillations in second-messenger cascades
    Timothy A Dunn
    Neurobiology Section, Division of Biological Sciences, University of California, San Diego, La Jolla, California 92093, USA
    J Neurosci 26:12807-15. 2006
    ..These results show a link between neural activity and an intracellular biochemical cascade associated with plasticity, axon guidance, and neural differentiation...
  70. ncbi Aptamers switch on fluorescence of triphenylmethane dyes
    Jeremy R Babendure
    Department of Pharmacology, Howard Hughes Medical Institute, University of California San Diego, La Jolla, CA 92093 0647, USA
    J Am Chem Soc 125:14716-7. 2003
    ..Such sequence-specific fluorescence labeling of short RNA motifs is a first step toward genetically encodable fusion tags for imaging selected RNAs in vitro and in cells...
  71. pmc Calcium-dependent regulation of protein kinase D revealed by a genetically encoded kinase activity reporter
    Maya T Kunkel
    Department of Pharmacology and Howard Hughes Medical Institute, University of California at San Diego, La Jolla, California 92093, USA
    J Biol Chem 282:6733-42. 2007
    ..These data reveal that Ca(2+) is a major contributor to the initiation of PKD signaling through positive feedback regulation of diacylglycerol production, unveiling a new mechanism in PKD activation...

Research Grants25

  1. Advanced Probes and Targeting for Multiscale Microscopy
    Roger Y Tsien; Fiscal Year: 2010
    ....
  2. DYNAMICS OF SIGNAL TRANSDUCTION IN NEURONS
    Roger Tsien; Fiscal Year: 2004
    ..Such new techniques should help explore the biochemical mechanisms of long-term depression and a newly discovered form of long-term potentiation of synapses between parallel fibers and Purkinje neurons in young adult cerebellar slices. ..
  3. DYNAMICS OF SIGNAL TRANSDUCTION IN NEURONS
    Roger Tsien; Fiscal Year: 2005
    ..Such new techniques should help explore the biochemical mechanisms of long-term depression and a newly discovered form of long-term potentiation of synapses between parallel fibers and Purkinje neurons in young adult cerebellar slices. ..
  4. DYNAMICS OF SIGNAL TRANSDUCTION IN NEURONS
    Roger Tsien; Fiscal Year: 2006
    ..Such new techniques should help explore the biochemical mechanisms of long-term depression and a newly discovered form of long-term potentiation of synapses between parallel fibers and Purkinje neurons in young adult cerebellar slices. ..
  5. DYNAMICS OF SIGNAL TRANSDUCTION IN NEURONS
    Roger Tsien; Fiscal Year: 2007
    ..Such tools will help elucidate mechanisms of neuronal information processing, communication, lifecycles, control of behavior, and pathological injury. ..
  6. GENETICALLY TARGETABLE LABELS FOR LIGHT AND EM
    Roger Tsien; Fiscal Year: 2007
    ..These activities benefit from the combination of this applicant teams expertise in chemistry, imaging instrument development, cell and molecular biology, physics and biophysics. ..
  7. GENETICALLY TARGETABLE LABELS FOR LIGHT AND EM
    Roger Tsien; Fiscal Year: 2006
    ..These activities benefit from the combination of this applicant teams expertise in chemistry, imaging instrument development, cell and molecular biology, physics and biophysics. ..
  8. Advanced Probes and Targeting for Multiscale Microscopy
    Roger Tsien; Fiscal Year: 2009
    ....
  9. DYNAMICS OF SIGNAL TRANSDUCTION IN NEURONS
    Roger Tsien; Fiscal Year: 2003
    ..Such new techniques should help explore the biochemical mechanisms of long-term depression and a newly discovered form of long-term potentiation of synapses between parallel fibers and Purkinje neurons in young adult cerebellar slices. ..
  10. DYNAMICS OF SIGNAL TRANSDUCTION IN NEURONS
    Roger Tsien; Fiscal Year: 2002
    ..Such new techniques should help explore the biochemical mechanisms of long-term depression and a newly discovered form of long-term potentiation of synapses between parallel fibers and Purkinje neurons in young adult cerebellar slices. ..
  11. DYNAMICS OF SIGNAL TRANSDUCTION IN NEURONS
    Roger Tsien; Fiscal Year: 2000
    ....
  12. DYNAMICS OF SIGNAL TRANSDUCTION IN NEURONS
    Roger Tsien; Fiscal Year: 1999
    ....
  13. DYNAMICS OF IONIC MESSENGERS IN NEURONS
    Roger Tsien; Fiscal Year: 1990
    ....
  14. DYNAMICS OF IONIC MESSENGERS IN NEURONS
    Roger Tsien; Fiscal Year: 1991
    ....
  15. DYNAMICS OF IONIC MESSENGERS IN NEURONS
    Roger Tsien; Fiscal Year: 1992
    ....
  16. DYNAMICS OF IONIC MESSENGERS IN NEURONS
    Roger Tsien; Fiscal Year: 1993
    ....
  17. Advanced Probes and Targeting for Multiscale Microscopy
    Roger Tsien; Fiscal Year: 2009
    ....