Billy Tsai

Summary

Affiliation: University of Michigan
Country: USA

Publications

  1. doi request reprint Cellular entry of polyomaviruses
    Billy Tsai
    Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI, 48109, USA
    Curr Top Microbiol Immunol 343:177-94. 2010
  2. ncbi request reprint Penetration of nonenveloped viruses into the cytoplasm
    Billy Tsai
    Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI 48109, USA
    Annu Rev Cell Dev Biol 23:23-43. 2007
  3. ncbi request reprint A virus takes an "L" turn to find its receptor
    Billy Tsai
    Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI 48109, USA
    Cell Host Microbe 8:301-2. 2010
  4. pmc A lipid receptor sorts polyomavirus from the endolysosome to the endoplasmic reticulum to cause infection
    Mengding Qian
    Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, Michigan, United States of America
    PLoS Pathog 5:e1000465. 2009
  5. pmc The E3 ubiquitin ligases Hrd1 and gp78 bind to and promote cholera toxin retro-translocation
    Kaleena M Bernardi
    Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI 48109, USA
    Mol Biol Cell 21:140-51. 2010
  6. pmc Lipids and proteins act in opposing manners to regulate polyomavirus infection
    Mengding Qian
    Department of Cell and Developmental Biology, University of Michigan Medical School, 109 Zina Pitcher Place, Rm 3043, Ann Arbor, MI 48109, USA
    J Virol 84:9840-52. 2010
  7. pmc The ERdj5-Sel1L complex facilitates cholera toxin retrotranslocation
    Jeffrey M Williams
    Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI 48103, USA
    Mol Biol Cell 24:785-95. 2013
  8. pmc A large and intact viral particle penetrates the endoplasmic reticulum membrane to reach the cytosol
    Takamasa Inoue
    Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, Michigan, United States of America
    PLoS Pathog 7:e1002037. 2011
  9. pmc The Ero1alpha-PDI redox cycle regulates retro-translocation of cholera toxin
    Paul Moore
    Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI 48109, USA
    Mol Biol Cell 21:1305-13. 2010
  10. pmc Endoplasmic reticulum-dependent redox reactions control endoplasmic reticulum-associated degradation and pathogen entry
    Christopher P Walczak
    Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, Michigan, USA
    Antioxid Redox Signal 16:809-18. 2012

Research Grants

Collaborators

  • Paul Moore
  • Souren Mkrtchian
  • Wayne I Lencer
  • Yihong Ye
  • Peter Arvan
  • Tom Rapoport
  • Takamasa Inoue
  • Kaleena M Bernardi
  • Emily K Rainey-Barger
  • Jeffrey M Williams
  • Michele L Forster
  • Christopher P Walczak
  • Mengding Qian
  • Brian Magnuson
  • Mengxi Jiang
  • Michael J Imperiale
  • Jonathan A Low
  • Aric Schultz
  • Lindsey Banks
  • Emmanuel J Wiertz
  • Marjolein Kikkert
  • Sjaak van Voorden
  • Dawen Cai
  • Johanna R Abend
  • Kristen J Verhey
  • James J Mahn
  • Young Nam Park
  • Kelsey Sivick
  • Thomas Benjamin
  • Mikhail Baryshev
  • Emily K Rainey

Detail Information

Publications26

  1. doi request reprint Cellular entry of polyomaviruses
    Billy Tsai
    Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI, 48109, USA
    Curr Top Microbiol Immunol 343:177-94. 2010
    ..Thus, as Pys and bacterial toxins hijack similar cellular machineries during infection, a general principle appears to guide their entry into host cells...
  2. ncbi request reprint Penetration of nonenveloped viruses into the cytoplasm
    Billy Tsai
    Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI 48109, USA
    Annu Rev Cell Dev Biol 23:23-43. 2007
    ..Moreover, higher-resolution structures of penetration intermediates, particularly those solved in complex with membranes, would provide important molecular details into this process...
  3. ncbi request reprint A virus takes an "L" turn to find its receptor
    Billy Tsai
    Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI 48109, USA
    Cell Host Microbe 8:301-2. 2010
    ..In this issue of Cell Host & Microbe, Neu et al. (2010) identify a receptor motif that engages JC virus, a human polyomavirus known to cause progressive multifocal leukoencephalopathy in immunocompromised individuals...
  4. pmc A lipid receptor sorts polyomavirus from the endolysosome to the endoplasmic reticulum to cause infection
    Mengding Qian
    Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, Michigan, United States of America
    PLoS Pathog 5:e1000465. 2009
    ..Our results provide a rationale for transport of Py through the endolysosome, demonstrate a novel endolysosome-to-ER transport pathway that is regulated by a lipid, and implicate ganglioside binding as a general ER targeting mechanism...
  5. pmc The E3 ubiquitin ligases Hrd1 and gp78 bind to and promote cholera toxin retro-translocation
    Kaleena M Bernardi
    Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI 48109, USA
    Mol Biol Cell 21:140-51. 2010
    ....
  6. pmc Lipids and proteins act in opposing manners to regulate polyomavirus infection
    Mengding Qian
    Department of Cell and Developmental Biology, University of Michigan Medical School, 109 Zina Pitcher Place, Rm 3043, Ann Arbor, MI 48109, USA
    J Virol 84:9840-52. 2010
    ..Thus, glycolipids and glycoproteins, two major constituents of the plasma membrane, execute opposing functions in regulating infection by a defined virus...
  7. pmc The ERdj5-Sel1L complex facilitates cholera toxin retrotranslocation
    Jeffrey M Williams
    Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI 48103, USA
    Mol Biol Cell 24:785-95. 2013
    ..We postulate this scenario enables the Hrd1-associated retrotranslocation machinery to capture the toxin efficiently once the toxin is released from BiP...
  8. pmc A large and intact viral particle penetrates the endoplasmic reticulum membrane to reach the cytosol
    Takamasa Inoue
    Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, Michigan, United States of America
    PLoS Pathog 7:e1002037. 2011
    ..They also suggest that the ER membrane supports passage of a large particle, potentially through either a sizeable protein-conducting channel or the lipid bilayer...
  9. pmc The Ero1alpha-PDI redox cycle regulates retro-translocation of cholera toxin
    Paul Moore
    Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI 48109, USA
    Mol Biol Cell 21:1305-13. 2010
    ..These findings demonstrate that an appropriate Ero1alpha-PDI ratio is critical for regulating the binding-release cycle of CTA1 by PDI during retro-translocation, and implicate PDI's redox state in targeting it to the retro-translocon...
  10. pmc Endoplasmic reticulum-dependent redox reactions control endoplasmic reticulum-associated degradation and pathogen entry
    Christopher P Walczak
    Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, Michigan, USA
    Antioxid Redox Signal 16:809-18. 2012
    ..Strikingly, various pathogenic viruses and toxins co-opt these redox components to reach the cytosol during entry. These redox factors thus regulate critical cellular homeostasis and host-pathogen interactions...
  11. pmc Generating an unfoldase from thioredoxin-like domains
    Michele L Forster
    Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI 48109, USA
    J Biol Chem 284:13045-56. 2009
    ..Thus, we propose that generating an unfoldase from thioredoxin-like domains requires the bb'(x) domains of PDI followed by an a' domain but not preceded by an inhibitory a domain...
  12. pmc Derlin-1 facilitates the retro-translocation of cholera toxin
    Kaleena M Bernardi
    Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI 48109, USA
    Mol Biol Cell 19:877-84. 2008
    ..CTB may play a role in this process by targeting the holotoxin to Derlin-1, enabling the Derlin-1-bound PDI to unfold the A1 subunit and prepare it for transport...
  13. pmc How viruses use the endoplasmic reticulum for entry, replication, and assembly
    Takamasa Inoue
    Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI 48103, USA
    Cold Spring Harb Perspect Biol 5:a013250. 2013
    ..An interdisciplinary approach, using rigorous biochemical and cell biological assays coupled with advanced microscopy strategies, will push to the next level our understanding of the virus-ER interaction during infection...
  14. pmc A chaperone-activated nonenveloped virus perforates the physiologically relevant endoplasmic reticulum membrane
    Emily K Rainey-Barger
    Department of Cell and Developmental Biology, University of Michigan Medical School, 109 Zina Pitcher Place, Rm 3043, Ann Arbor, MI 48109, USA
    J Virol 81:12996-3004. 2007
    ..Our data thus link the activity of a cellular factor on a nonenveloped virus to the membrane perforation event and identify a viral component that mediates this process...
  15. pmc A deubiquitinase negatively regulates retro-translocation of nonubiquitinated substrates
    Kaleena M Bernardi
    Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI 48103 Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109
    Mol Biol Cell 24:3545-56. 2013
    ..Our findings reveal that a cytosolic DUB exerts a negative function during retro-translocation of nonubiquitinated substrates, potentially by acting on elements of the ERAD machinery. ..
  16. pmc The C-terminal domain of ERp29 mediates polyomavirus binding, unfolding, and infection
    Emily K Rainey-Barger
    Department of Cell and Developmental Biology, University of Michigan Medical School, 109 Zina Pitcher Place, Rm 3043, Ann Arbor, MI 48109, USA
    J Virol 83:1483-91. 2009
    ..Our data thus demonstrate that the ERp29 CTD plays a crucial role in PyV unfolding and infection, likely by serving as part of a substrate-binding domain...
  17. pmc Dimerization of ERp29, a PDI-like protein, is essential for its diverse functions
    Emily K Rainey-Barger
    Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI 48109, USA
    Mol Biol Cell 18:1253-60. 2007
    ..We show that this escort function likewise depends on ERp29 dimerization. Thus our data demonstrate that dimerization of a PDI-like protein acts to regulate its diverse ER activities...
  18. pmc A PDI family network acts distinctly and coordinately with ERp29 to facilitate polyomavirus infection
    Christopher P Walczak
    Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI 48109, USA
    J Virol 85:2386-96. 2011
    ..This study reveals how a PDI family functions coordinately and distinctly to promote Py infection and pinpoints a role of viral cysteines in this process...
  19. pmc Early events during BK virus entry and disassembly
    Mengxi Jiang
    Department of Microbiology and Immunology and Comprehensive Cancer Center, University of Michigan Medical School, Ann Arbor, Michigan 48109 5620, USA
    J Virol 83:1350-8. 2009
    ..Finally, we show that proteasome function is also linked to BKV infection and capsid rearrangement. These results indicate that BKV early entry and disassembly are highly regulated processes involving multiple cellular components...
  20. pmc Identification of gangliosides GD1b and GT1b as receptors for BK virus
    Jonathan A Low
    Department of Microbiology and Immunology, University of Michigan Medical School, 1500 E Medical Center Dr, 6304 Cancer Center, Ann Arbor, MI 48109 0942, USA
    J Virol 80:1361-6. 2006
    ..These data demonstrate that BKV uses the gangliosides GT1b and GD1b as receptors and passes through the ER on the way to the nucleus...
  21. doi request reprint How viruses and toxins disassemble to enter host cells
    Takamasa Inoue
    Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA
    Annu Rev Microbiol 65:287-305. 2011
    ..Where appropriate, we also underscore their differences. Our major intention is to draw together the fields of viral and toxin cell entry by using lessons gleaned from each field to inform and benefit one another...
  22. pmc Protein disulfide isomerase-like proteins play opposing roles during retrotranslocation
    Michele L Forster
    Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI 48109, USA
    J Cell Biol 173:853-9. 2006
    ..Thus, our data identify PDI family proteins that play opposing roles in ER quality control and establish an assay to further delineate the mechanism of CT retrotranslocation...
  23. pmc Establishment of an In Vitro Transport Assay That Reveals Mechanistic Differences in Cytosolic Events Controlling Cholera Toxin and T-Cell Receptor α Retro-Translocation
    Paul Moore
    Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, Michigan, United States of America
    PLoS ONE 8:e75801. 2013
    ..Our results reveal mechanistic insights into cytosolic events controlling CTA1 and TCRα retro-translocation, and provide a reliable tool to further probe this process. ..
  24. ncbi request reprint ERp29 triggers a conformational change in polyomavirus to stimulate membrane binding
    Brian Magnuson
    Department of Cell and Developmental Biology, University of Michigan Medical School, 4643 Medical Sciences II, 1335 East Catherine Street, Ann Arbor, Michigan 48109, USA
    Mol Cell 20:289-300. 2005
    ..Our data thus identify an ER factor that mediates membrane penetration of a nonenveloped virus and suggest that PDI family members are generally involved in ER remodeling reactions...
  25. ncbi request reprint The intracellular voyage of cholera toxin: going retro
    Wayne I Lencer
    GI Cell Biology, Children s Hospital and the Department of Pediatrics, Harvard Medical School, Boston, MA 02115, USA
    Trends Biochem Sci 28:639-45. 2003
    ..Upon entering the cytosol, the A1-chain rapidly refolds, avoids the proteasome and induces toxicity...
  26. pmc Gangliosides are receptors for murine polyoma virus and SV40
    Billy Tsai
    Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    EMBO J 22:4346-55. 2003
    ..Lipid binding sites for polyoma are shown to be present in rough ER membranes, suggesting that the virus travel with the ganglioside(s) from the plasma membranes to the ER...

Research Grants7

  1. Transport of polyomavirus across the ER membrane
    Billy Tsai; Fiscal Year: 2007
    ..As many of Py's structurally-related viruses are human pathogens, such as the JC, BK, and papilloma viruses, the lessons gleaned from the cellular entry mechanism of Py may be applied to a broader spectrum of human diseases. ..
  2. Transport of polyomavirus across the ER membrane
    Billy Tsai; Fiscal Year: 2009
    ..As many of Py's structurally-related viruses are human pathogens, such as the JC, BK, and papilloma viruses, the lessons gleaned from the cellular entry mechanism of Py may be applied to a broader spectrum of human diseases. ..
  3. Transport of polyomavirus across the ER membrane
    Billy Tsai; Fiscal Year: 2010
    ..As many of Py's structurally-related viruses are human pathogens, such as the JC, BK, and papilloma viruses, the lessons gleaned from the cellular entry mechanism of Py may be applied to a broader spectrum of human diseases. ..
  4. Mechanism of cholera toxin retro-translocation
    Billy Tsai; Fiscal Year: 2010
    ..However, the molecular details by which CT penetrate the ER membrane is not clear. We intend to clarify these processes in this application. ..
  5. Transport of polyomavirus across the ER membrane
    Billy Tsai; Fiscal Year: 2009
    ..As many of Py's structurally-related viruses are human pathogens, such as the JC, BK, and papilloma viruses, the lessons gleaned from the cellular entry mechanism of Py may be applied to a broader spectrum of human diseases. ..