Affiliation: University of California
Grimsey N, Lin Y, Narala R, Rada C, Mejia Pena H, Trejo J. G protein-coupled receptors activate p38 MAPK via a non-canonical TAB1-TAB2- and TAB1-TAB3-dependent pathway in endothelial cells. J Biol Chem. 2019;294:5867-5878 pubmed publisher
..We conclude that multiple GPCR agonists utilize non-canonical TAB1-TAB2 and TAB1-TAB3-dependent p38 activation to promote endothelial inflammatory responses. ..
Grimsey N, Narala R, Rada C, Mehta S, Stephens B, Kufareva I, et al
. A Tyrosine Switch on NEDD4-2 E3 Ligase Transmits GPCR Inflammatory Signaling. Cell Rep. 2018;24:3312-3323.e5 pubmed publisher
..These studies reveal a novel role for c-Src in GPCR-induced NEDD4-2 activation, which is critical for driving ubiquitin-mediated p38 inflammatory signaling. ..
Grimsey N, Lin H, Trejo J. Endosomal signaling by protease-activated receptors. Methods Enzymol. 2014;535:389-401 pubmed publisher
..Here, we provide an overview of methods and strategies used to examine endosomal signaling by PARs. ..
Arakaki A, Pan W, Trejo J. GPCRs in Cancer: Protease-Activated Receptors, Endocytic Adaptors and Signaling. Int J Mol Sci. 2018;19: pubmed publisher
Grimsey N, Aguilar B, Smith T, Le P, Soohoo A, Puthenveedu M, et al
. Ubiquitin plays an atypical role in GPCR-induced p38 MAP kinase activation on endosomes. J Cell Biol. 2015;210:1117-31 pubmed publisher
..These studies define an atypical ubiquitin-mediated signaling pathway used by a subset of GPCRs that regulates endosomal p38 signaling and endothelial barrier disruption. ..
Chen B, Soto A, Coronel L, Goss A, van Ryn J, Trejo J. Characterization of thrombin-bound dabigatran effects on protease-activated receptor-1 expression and signaling in vitro. Mol Pharmacol. 2015;88:95-105 pubmed publisher
Arakaki A, Pan W, Lin H, Trejo J. The α-arrestin ARRDC3 suppresses breast carcinoma invasion by regulating G protein-coupled receptor lysosomal sorting and signaling. J Biol Chem. 2018;293:3350-3362 pubmed publisher
..These findings are the first to identify a critical link between the tumor suppressor ARRDC3 and regulation of GPCR trafficking and signaling in breast cancer. ..
Soto A, Smith T, Chen B, Bhattacharya S, Cordova I, Kenakin T, et al
. N-linked glycosylation of protease-activated receptor-1 at extracellular loop 2 regulates G-protein signaling bias. Proc Natl Acad Sci U S A. 2015;112:E3600-8 pubmed publisher
Dores M, Lin H, J Grimsey N, Méndez F, Trejo J. The Î±-arrestin ARRDC3 mediates ALIX ubiquitination and G protein-coupled receptor lysosomal sorting. Mol Biol Cell. 2015;26:4660-73 pubmed publisher
..These findings demonstrate a new role for the Î±-arrestin ARRDC3 and the E3 ubiquitin ligase WWP2 in regulation of ALIX ubiquitination and lysosomal sorting of GPCRs. ..