L S Tobacman

Summary

Affiliation: University of Iowa
Country: USA

Publications

  1. ncbi request reprint A new model of cooperative myosin-thin filament binding
    L S Tobacman
    Departments of Internal Medicine and Biochemistry, The University of Iowa, Iowa City, Iowa 52242, USA
    J Biol Chem 275:27587-93. 2000
  2. ncbi request reprint The troponin tail domain promotes a conformational state of the thin filament that suppresses myosin activity
    Larry S Tobacman
    Department of Internal Medicine, The University of Iowa, Iowa City, Iowa 52242, USA
    J Biol Chem 277:27636-42. 2002
  3. ncbi request reprint Roles for the troponin tail domain in thin filament assembly and regulation. A deletional study of cardiac troponin T
    A Hinkle
    Department of Internal Medicine, University of Iowa, Iowa City, Iowa 52242, USA
    J Biol Chem 274:7157-64. 1999
  4. ncbi request reprint Modulation of myosin function by isoform-specific properties of Saccharomyces cerevisiae and muscle tropomyosins
    J Strand
    Departments of Internal Medicine and Biochemistry, The University of Iowa, Iowa City, Iowa 52242, USA
    J Biol Chem 276:34832-9. 2001
  5. pmc Effects of cardiac thin filament Ca2+: statistical mechanical analysis of a troponin C site II mutant
    Q Huynh
    Department of Internal Medicine, University of Iowa, Iowa City 52242, USA
    Biophys J 70:1447-55. 1996
  6. ncbi request reprint Functional consequences of troponin T mutations found in hypertrophic cardiomyopathy
    L S Tobacman
    Department of Internal Medicine, The University of Iowa, Iowa City, Iowa 52242, USA
    J Biol Chem 274:28363-70. 1999
  7. ncbi request reprint Effects of a cardiomyopathy-causing troponin t mutation on thin filament function and structure
    J Burhop
    Departments of Internal Medicine and Biochemistry, University of Iowa, Iowa City, Iowa 52242, USA
    J Biol Chem 276:20788-94. 2001
  8. pmc Mini-thin filaments regulated by troponin-tropomyosin
    Huiyu Gong
    Department of Internal Medicine, University of Iowa, Iowa City, IA 52242, USA
    Proc Natl Acad Sci U S A 102:656-61. 2005
  9. ncbi request reprint Cardiomyopathic tropomyosin mutations that increase thin filament Ca2+ sensitivity and tropomyosin N-domain flexibility
    Mark J Heller
    Departments of Internal Medicine and Biochemistry, University of Iowa, Iowa City, IA 52242, USA
    J Biol Chem 278:41742-8. 2003
  10. pmc Temperature-dependence of isometric tension and cross-bridge kinetics of cardiac muscle fibers reconstituted with a tropomyosin internal deletion mutant
    Xiaoying Lu
    Department of Anatomy and Cell Biology, University of Iowa, Iowa City, IA, USA
    Biophys J 91:4230-40. 2006

Collaborators

  • E Homsher
  • WILLIAM J LEHMAN
  • ROGER W CRAIG
  • Masataka Kawai
  • Carl A Morris
  • Kenneth C Holmes
  • Dmitry Pavlov
  • Alnoor Pirani
  • Victoria Hatch
  • Xiaoying Lu
  • Chen Xu
  • Jerry H Brown
  • Agnieszka Galinska-Rakoczy
  • Katrina J V Poole
  • Huiyu Gong
  • Anthony Cammarato
  • Carolyn Cohen
  • Ludmilla Reshetnikova
  • Ashley Hinkle
  • Mark J Heller
  • Yu Li
  • J Burhop
  • J Strand
  • Hyunsuk Jung
  • Patti Engel
  • A Hinkle
  • Paul M G Curmi
  • Gerd Rosenbaum
  • Michael Lorenz
  • Maia V Vinogradova
  • William A King
  • Robert J Fletterick
  • Gwyndaf Evans
  • Laith Ali
  • C A Butters
  • Rama D Yammani
  • Howard Robinson
  • Zhaocai Zhou
  • John C Sparrow
  • Judith Saide
  • Mahta Nili
  • James Strand
  • Suet Mui
  • Q Huynh
  • M Nili
  • M Rosol
  • A Goranson
  • J M Leiden

Detail Information

Publications21

  1. ncbi request reprint A new model of cooperative myosin-thin filament binding
    L S Tobacman
    Departments of Internal Medicine and Biochemistry, The University of Iowa, Iowa City, Iowa 52242, USA
    J Biol Chem 275:27587-93. 2000
    ..Finally, initiation of a shift in tropomyosin position is 100-fold more difficult than is its extension from one actin to the next, producing the myosin binding cooperativity that underlies cooperative activation of muscle contraction...
  2. ncbi request reprint The troponin tail domain promotes a conformational state of the thin filament that suppresses myosin activity
    Larry S Tobacman
    Department of Internal Medicine, The University of Iowa, Iowa City, Iowa 52242, USA
    J Biol Chem 277:27636-42. 2002
    ..All of these results indicate that portions of the troponin tail domain, and not only troponin I, contribute to the positioning of tropomyosin on the actin outer domain, thereby inhibiting muscle contraction in the absence of Ca(2+)...
  3. ncbi request reprint Roles for the troponin tail domain in thin filament assembly and regulation. A deletional study of cardiac troponin T
    A Hinkle
    Department of Internal Medicine, University of Iowa, Iowa City, Iowa 52242, USA
    J Biol Chem 274:7157-64. 1999
    ....
  4. ncbi request reprint Modulation of myosin function by isoform-specific properties of Saccharomyces cerevisiae and muscle tropomyosins
    J Strand
    Departments of Internal Medicine and Biochemistry, The University of Iowa, Iowa City, Iowa 52242, USA
    J Biol Chem 276:34832-9. 2001
    ....
  5. pmc Effects of cardiac thin filament Ca2+: statistical mechanical analysis of a troponin C site II mutant
    Q Huynh
    Department of Internal Medicine, University of Iowa, Iowa City 52242, USA
    Biophys J 70:1447-55. 1996
    ..However, these indirect TnC-TnC interactions are weak, indicating that the cooperativity of muscle activation by Ca2+ requires other sources of cooperativity...
  6. ncbi request reprint Functional consequences of troponin T mutations found in hypertrophic cardiomyopathy
    L S Tobacman
    Department of Internal Medicine, The University of Iowa, Iowa City, Iowa 52242, USA
    J Biol Chem 274:28363-70. 1999
    ..Enhancement as well as impairment of contractile protein function is observed, suggesting that TnT, including the troponin tail region, modulates the regulation of cardiac contraction...
  7. ncbi request reprint Effects of a cardiomyopathy-causing troponin t mutation on thin filament function and structure
    J Burhop
    Departments of Internal Medicine and Biochemistry, University of Iowa, Iowa City, Iowa 52242, USA
    J Biol Chem 276:20788-94. 2001
    ..Development of hypertrophic cardiomyopathy can thus be traced to a defect in the primary mechanism controlling cardiac contraction, switching between different conformations of the thin filament...
  8. pmc Mini-thin filaments regulated by troponin-tropomyosin
    Huiyu Gong
    Department of Internal Medicine, University of Iowa, Iowa City, IA 52242, USA
    Proc Natl Acad Sci U S A 102:656-61. 2005
    ..Two-stranded single regulatory units therefore may be sufficient for myosin-mediated Ca2+-binding cooperativity. Functional mini-thin filaments are well suited for biochemical and structural analysis of thin-filament regulation...
  9. ncbi request reprint Cardiomyopathic tropomyosin mutations that increase thin filament Ca2+ sensitivity and tropomyosin N-domain flexibility
    Mark J Heller
    Departments of Internal Medicine and Biochemistry, University of Iowa, Iowa City, IA 52242, USA
    J Biol Chem 278:41742-8. 2003
    ....
  10. pmc Temperature-dependence of isometric tension and cross-bridge kinetics of cardiac muscle fibers reconstituted with a tropomyosin internal deletion mutant
    Xiaoying Lu
    Department of Anatomy and Cell Biology, University of Iowa, Iowa City, IA, USA
    Biophys J 91:4230-40. 2006
    ..We conclude that Tm affects the conformation of actin so as to increase the area of hydrophobic interaction between actin and myosin molecules...
  11. ncbi request reprint Folding and function of the troponin tail domain. Effects of cardiomyopathic troponin T mutations
    Ashley Hinkle
    Department of Internal Medicine, University of Iowa, Iowa City, Iowa 52242, USA
    J Biol Chem 278:506-13. 2003
    ..We suggest that most cardiomyopathic mutations in the troponin tail alter muscle function indirectly, by perturbing interactions between troponin and tropomyosin requisite for the complex effects of these proteins on myosin...
  12. pmc Effects of tropomyosin internal deletion Delta23Tm on isometric tension and the cross-bridge kinetics in bovine myocardium
    Xiaoying Lu
    Department of Anatomy and Cell Biology, University of Iowa, Iowa City, IA 52242, USA
    J Physiol 553:457-71. 2003
    ..In Delta23Tm, the interface may not have a perfect stereospecific match so that the tension- and stiffness-generating capacity is greatly diminished...
  13. pmc Structure of the mid-region of tropomyosin: bending and binding sites for actin
    Jerry H Brown
    Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, MA 02454 9110, USA
    Proc Natl Acad Sci U S A 102:18878-83. 2005
    ..e., the so-called "alpha zones") of tropomyosin axially aligned with subdomain 3 of each monomer in F-actin...
  14. ncbi request reprint Single particle analysis of relaxed and activated muscle thin filaments
    Alnoor Pirani
    Department of Physiology and Biophysics, Boston University School of Medicine, 715 Albany Street, Boston, MA 02118, USA
    J Mol Biol 346:761-72. 2005
    ..This flexibility in the localization of tropomyosin may provide a means of sterically regulating contraction at low energy cost...
  15. pmc Structural basis for the regulation of muscle contraction by troponin and tropomyosin
    Agnieszka Galinska-Rakoczy
    Department of Physiology and Biophysics, Boston University School of Medicine, 715 Albany Street, Boston, MA 02118, USA
    J Mol Biol 379:929-35. 2008
    ..Thus, the observations reported reveal the structural mechanism responsible for troponin-tropomyosin-mediated steric interference of actin-myosin interaction that regulates muscle contraction...
  16. ncbi request reprint The regulation of subtilisin-cleaved actin by tropomyosin/troponin
    Dmitry Pavlov
    Department of Chemistry and Biochemistry, University of California, Los Angeles, California 90095, USA
    J Biol Chem 278:5517-22. 2003
    ..In addition, the unimpaired calcium-sensitive regulation of cleaved actin indicates that subdomain 2 conformation does not play an essential role in the regulation process...
  17. pmc The crystal structure of the C-terminal fragment of striated-muscle alpha-tropomyosin reveals a key troponin T recognition site
    Yu Li
    Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, MA 02454 9110, USA
    Proc Natl Acad Sci U S A 99:7378-83. 2002
    ..These results reveal a specific recognition site for troponin T and clarify the physical basis for the unique regulatory mechanism of striated muscles...
  18. pmc Drosophila muscle regulation characterized by electron microscopy and three-dimensional reconstruction of thin filament mutants
    Anthony Cammarato
    Department of Physiology and Biophysics, Boston University School of Medicine, Boston, Massachusetts 02118 2526, USA
    Biophys J 86:1618-24. 2004
    ..The interaction of mutant troponin I with Drosophila troponin C is discussed, along with functional differences between troponin C from Drosophila and vertebrates...
  19. ncbi request reprint An atomic model of the thin filament in the relaxed and Ca2+-activated states
    Alnoor Pirani
    Department of Physiology and Biophysics, Boston University School of Medicine, Boston, MA 02118, USA
    J Mol Biol 357:707-17. 2006
    ..Our observations suggest a simple model for muscle regulation in which troponin affects the charge balance on actin and hence tropomyosin position...
  20. ncbi request reprint A comparison of muscle thin filament models obtained from electron microscopy reconstructions and low-angle X-ray fibre diagrams from non-overlap muscle
    Katrina J V Poole
    Arbeitsgruppe Biophysik, Max Planck Institut fur medizinische Forschung, 69120 Heidelberg, Germany
    J Struct Biol 155:273-84. 2006
    ....
  21. pmc Thin filament activation and unloaded shortening velocity of rabbit skinned muscle fibres
    Carl A Morris
    Department of Physiology, School of Medicine, University of California, Los Angeles, CA 90095, USA
    J Physiol 550:205-15. 2003
    ....