Research Topics
| L S TobacmanSummaryAffiliation: University of Iowa Country: USA Publications
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Publications
A new model of cooperative myosin-thin filament bindingL S Tobacman
Departments of Internal Medicine and Biochemistry, The University of Iowa, Iowa City, Iowa 52242, USA
J Biol Chem 275:27587-93. 2000..Finally, initiation of a shift in tropomyosin position is 100-fold more difficult than is its extension from one actin to the next, producing the myosin binding cooperativity that underlies cooperative activation of muscle contraction...- The troponin tail domain promotes a conformational state of the thin filament that suppresses myosin activityLarry S Tobacman
Department of Internal Medicine, The University of Iowa, Iowa City, Iowa 52242, USA
J Biol Chem 277:27636-42. 2002..All of these results indicate that portions of the troponin tail domain, and not only troponin I, contribute to the positioning of tropomyosin on the actin outer domain, thereby inhibiting muscle contraction in the absence of Ca(2+)... - Roles for the troponin tail domain in thin filament assembly and regulation. A deletional study of cardiac troponin TA Hinkle
Department of Internal Medicine, University of Iowa, Iowa City, Iowa 52242, USA
J Biol Chem 274:7157-64. 1999.... - Modulation of myosin function by isoform-specific properties of Saccharomyces cerevisiae and muscle tropomyosinsJ Strand
Departments of Internal Medicine and Biochemistry, The University of Iowa, Iowa City, Iowa 52242, USA
J Biol Chem 276:34832-9. 2001.... 
Effects of cardiac thin filament Ca2+: statistical mechanical analysis of a troponin C site II mutantQ Huynh
Department of Internal Medicine, University of Iowa, Iowa City 52242, USA
Biophys J 70:1447-55. 1996..However, these indirect TnC-TnC interactions are weak, indicating that the cooperativity of muscle activation by Ca2+ requires other sources of cooperativity...- Functional consequences of troponin T mutations found in hypertrophic cardiomyopathyL S Tobacman
Department of Internal Medicine, The University of Iowa, Iowa City, Iowa 52242, USA
J Biol Chem 274:28363-70. 1999..Enhancement as well as impairment of contractile protein function is observed, suggesting that TnT, including the troponin tail region, modulates the regulation of cardiac contraction... - Effects of a cardiomyopathy-causing troponin t mutation on thin filament function and structureJ Burhop
Departments of Internal Medicine and Biochemistry, University of Iowa, Iowa City, Iowa 52242, USA
J Biol Chem 276:20788-94. 2001..Development of hypertrophic cardiomyopathy can thus be traced to a defect in the primary mechanism controlling cardiac contraction, switching between different conformations of the thin filament... - Mini-thin filaments regulated by troponin-tropomyosinHuiyu Gong
Department of Internal Medicine, University of Iowa, Iowa City, IA 52242, USA
Proc Natl Acad Sci U S A 102:656-61. 2005..Two-stranded single regulatory units therefore may be sufficient for myosin-mediated Ca2+-binding cooperativity. Functional mini-thin filaments are well suited for biochemical and structural analysis of thin-filament regulation... - Cardiomyopathic tropomyosin mutations that increase thin filament Ca2+ sensitivity and tropomyosin N-domain flexibilityMark J Heller
Departments of Internal Medicine and Biochemistry, University of Iowa, Iowa City, IA 52242, USA
J Biol Chem 278:41742-8. 2003.... - Temperature-dependence of isometric tension and cross-bridge kinetics of cardiac muscle fibers reconstituted with a tropomyosin internal deletion mutantXiaoying Lu
Department of Anatomy and Cell Biology, University of Iowa, Iowa City, IA, USA
Biophys J 91:4230-40. 2006..We conclude that Tm affects the conformation of actin so as to increase the area of hydrophobic interaction between actin and myosin molecules... - Folding and function of the troponin tail domain. Effects of cardiomyopathic troponin T mutationsAshley Hinkle
Department of Internal Medicine, University of Iowa, Iowa City, Iowa 52242, USA
J Biol Chem 278:506-13. 2003..We suggest that most cardiomyopathic mutations in the troponin tail alter muscle function indirectly, by perturbing interactions between troponin and tropomyosin requisite for the complex effects of these proteins on myosin... - Effects of tropomyosin internal deletion Delta23Tm on isometric tension and the cross-bridge kinetics in bovine myocardiumXiaoying Lu
Department of Anatomy and Cell Biology, University of Iowa, Iowa City, IA 52242, USA
J Physiol 553:457-71. 2003..In Delta23Tm, the interface may not have a perfect stereospecific match so that the tension- and stiffness-generating capacity is greatly diminished... - Structure of the mid-region of tropomyosin: bending and binding sites for actinJerry H Brown
Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, MA 02454 9110, USA
Proc Natl Acad Sci U S A 102:18878-83. 2005..e., the so-called "alpha zones") of tropomyosin axially aligned with subdomain 3 of each monomer in F-actin... - Single particle analysis of relaxed and activated muscle thin filamentsAlnoor Pirani
Department of Physiology and Biophysics, Boston University School of Medicine, 715 Albany Street, Boston, MA 02118, USA
J Mol Biol 346:761-72. 2005..This flexibility in the localization of tropomyosin may provide a means of sterically regulating contraction at low energy cost... - Structural basis for the regulation of muscle contraction by troponin and tropomyosinAgnieszka Galinska-Rakoczy
Department of Physiology and Biophysics, Boston University School of Medicine, 715 Albany Street, Boston, MA 02118, USA
J Mol Biol 379:929-35. 2008..Thus, the observations reported reveal the structural mechanism responsible for troponin-tropomyosin-mediated steric interference of actin-myosin interaction that regulates muscle contraction... - The regulation of subtilisin-cleaved actin by tropomyosin/troponinDmitry Pavlov
Department of Chemistry and Biochemistry, University of California, Los Angeles, California 90095, USA
J Biol Chem 278:5517-22. 2003..In addition, the unimpaired calcium-sensitive regulation of cleaved actin indicates that subdomain 2 conformation does not play an essential role in the regulation process... - The crystal structure of the C-terminal fragment of striated-muscle alpha-tropomyosin reveals a key troponin T recognition siteYu Li
Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, MA 02454-9110, USA
Proc Natl Acad Sci U S A 99:7378-83. 2002..These results reveal a specific recognition site for troponin T and clarify the physical basis for the unique regulatory mechanism of striated muscles... - Drosophila muscle regulation characterized by electron microscopy and three-dimensional reconstruction of thin filament mutantsAnthony Cammarato
Department of Physiology and Biophysics, Boston University School of Medicine, Boston, Massachusetts 02118 2526, USA
Biophys J 86:1618-24. 2004..The interaction of mutant troponin I with Drosophila troponin C is discussed, along with functional differences between troponin C from Drosophila and vertebrates... - An atomic model of the thin filament in the relaxed and Ca2+-activated statesAlnoor Pirani
Department of Physiology and Biophysics, Boston University School of Medicine, Boston, MA 02118, USA
J Mol Biol 357:707-17. 2006..Our observations suggest a simple model for muscle regulation in which troponin affects the charge balance on actin and hence tropomyosin position... - A comparison of muscle thin filament models obtained from electron microscopy reconstructions and low-angle X-ray fibre diagrams from non-overlap muscleKatrina J V Poole
Arbeitsgruppe Biophysik, , 69120 Heidelberg, Germany
J Struct Biol 155:273-84. 2006.... - Thin filament activation and unloaded shortening velocity of rabbit skinned muscle fibresCarl A Morris
Department of Physiology, School of Medicine, University of California, Los Angeles, CA 90095, USA
J Physiol 550:205-15. 2003....