LISA D TIMMONSSummaryAffiliation: University of Kansas Country: USA Publications
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Publications
Inducible systemic RNA silencing in Caenorhabditis elegansLisa Timmons
Department of Molecular Biosciences, The University of Kansas, Lawrence, Kansas 66045, USA
Mol Biol Cell 14:2972-83. 2003..elegans and can separate mechanisms underlying systemic RNA silencing into tissue-specific components. These data suggest that trafficking of RNA silencing signals in C. elegans is regulated by specific physiological and genetic factors...- Endogenous inhibitors of RNA interference in Caenorhabditis elegansLisa Timmons
Department of Molecular Biosciences, 1200 Sunnyside Avenue, University of Kansas, Lawrence, Kansas 66045, USA
Bioessays 26:715-8. 2004..Since ERI-1 is a conserved protein, development of procedures to enhance RNAi effectiveness in other systems may be possible... - ABC transporters and RNAi in Caenorhabditis elegansLISA D TIMMONS
Department of Molecular Biosciences, The University of Kansas, 1200 Sunnyside Avenue, 5041 Haworth Hall, Lawrence, KS 66045 7534, USA
J Bioenerg Biomembr 39:459-63. 2007..We will also describe unique roles for some transporter genes in endogenous RNAi-related processes... - Caenorhabditis elegans ABCRNAi transporters interact genetically with rde-2 and mut-7Prema Sundaram
Department of Molecular Biosciences, University of Kansas, Lawrence, Kansas 66045, USA
Genetics 178:801-14. 2008..From our results, we speculate that the coordinated activities of ABC(RNAi) transporters, through their effects on endogenous RNAi-related mechanisms, ultimately affect chromosome function and integrity... - The Caenorhabditis elegans rsd-2 and rsd-6 genes are required for chromosome functions during exposure to unfavorable environmentsWang Han
Department of Molecular Biosciences, University of Kansas, Lawrence, Kansas 66045, USA
Genetics 178:1875-93. 2008..Thus, the ultimate functions of rsd-2 and rsd-6 in systemic RNAi are remarkably complex and environmentally responsive... - ATP-binding cassette transporters are required for efficient RNA interference in Caenorhabditis elegansPrema Sundaram
Department of Molecular Biosciences, University of Kansas, Lawrence, KS 66045, USA
Mol Biol Cell 17:3678-88. 2006..elegans. Thus, the ability to mount a robust RNAi response to dsRNA depends upon the deployment of two ancient systems that respond to environmental assaults: RNAi mechanisms and membrane transport systems that use ABC proteins... - Delivery methods for RNA interference in C. elegansLisa Timmons
Department of Molecular Biosciences, University of Kansas, Lawrence, USA
Methods Mol Biol 351:119-25. 2006..This chapter describes four methods for delivery of double-stranded RNA to Caenorhabditis elegans (injection, feeding, soaking, and in vivo delivery), and suggests schemes that should facilitate detection of specific gene silencing... - Construction of plasmids for RNA interference and in vitro transcription of double-stranded RNALisa Timmons
Department of Molecular Biosciences, University of Kansas, Lawrence, USA
Methods Mol Biol 351:109-17. 2006..In this chapter, we describe how to generate DNAs that can be used as templates for transcription of dsRNA... - The long and short of siRNAsLisa Timmons
Department of Molecular Biosciences, University of Kansas, Lawrence, KS 66045, USA
Mol Cell 10:435-7. 2002..2002). This class has slower electrophoretic mobility than previously characterized siRNAs and may play an important role in transgene-induced systemic silencing and in methylation of endogenous retroelement DNA... - Methods for delivery of double-stranded RNA into Caenorhabditis elegansDawn Hull
Department of Molecular Biosciences, University of Kansas, Lawrence, KS, USA
Methods Mol Biol 265:23-58. 2004..elegans, techniques for dsRNA preparation, four basic methods for delivery of dsRNA to C. elegans (injection, soaking, feeding, and in vivo delivery), and we suggest schemes that should facilitate detection of specific gene silencing...
Research Grants
- INBRE: KU-L: PARTITION OF DSRNAS TO DAUGHTER CELLS FOLLOWING MITOSIS/MEIOSISLisa Timmons; Fiscal Year: 2006..We are using genetics and developmental biology approaches in C. elegans to uncover mechanisms that facilitate silencing in dividing cells of developing organisms. ..