Colin Thorpe

Summary

Affiliation: University of Delaware
Country: USA

Publications

  1. ncbi request reprint Generating disulfides in multicellular organisms: emerging roles for a new flavoprotein family
    Colin Thorpe
    Department of Chemistry and Biochemistry, University of Delaware, Newark, DE 19716, USA
    J Biol Chem 282:13929-33. 2007
  2. ncbi request reprint Sulfhydryl oxidases: emerging catalysts of protein disulfide bond formation in eukaryotes
    Colin Thorpe
    Department of Chemistry and Biochemistry, University of Delaware, Newark, DE 19716, USA
    Arch Biochem Biophys 405:1-12. 2002
  3. pmc Arsenic(III) species inhibit oxidative protein folding in vitro
    Danny Ramadan
    Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware 19716, USA
    Biochemistry 48:424-32. 2009
  4. pmc Flavin-linked Erv-family sulfhydryl oxidases release superoxide anion during catalytic turnover
    Vidyadhar N Daithankar
    Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware 19716 2522, United States
    Biochemistry 51:265-72. 2012
  5. pmc Human quiescin-sulfhydryl oxidase, QSOX1: probing internal redox steps by mutagenesis
    Erin J Heckler
    Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware 19716, USA
    Biochemistry 47:4955-63. 2008
  6. pmc A novel disulfide-rich protein motif from avian eggshell membranes
    Vamsi K Kodali
    Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware, United States of America
    PLoS ONE 6:e18187. 2011
  7. pmc (77)Se enrichment of proteins expands the biological NMR toolbox
    Stephanie A Schaefer
    136 Brown Laboratory, Department of Chemistry and Biochemistry, University of Delaware, Newark, DE 19716, USA
    J Mol Biol 425:222-31. 2013
  8. pmc Protein substrate discrimination in the quiescin sulfhydryl oxidase (QSOX) family
    Jennifer A Codding
    Department of Chemistry and Biochemistry, University of Delaware, Newark, DE 19716 2522, USA
    Biochemistry 51:4226-35. 2012
  9. pmc Quiescin sulfhydryl oxidase from Trypanosoma brucei: catalytic activity and mechanism of a QSOX family member with a single thioredoxin domain
    Vamsi K Kodali
    Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware 19716, USA
    Biochemistry 49:2075-85. 2010
  10. pmc Augmenter of liver regeneration: substrate specificity of a flavin-dependent oxidoreductase from the mitochondrial intermembrane space
    Vidyadhar N Daithankar
    Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware 19716, USA
    Biochemistry 48:4828-37. 2009

Research Grants

  1. Flavoproteins in Oxidative Protein Folding
    Colin Thorpe; Fiscal Year: 2010
  2. FLAVOPROTEINS IN FATTY ACID METABOLISM
    Colin Thorpe; Fiscal Year: 1999
  3. FLAVOPROTEINS IN FATTY ACID METABOLISM
    Colin Thorpe; Fiscal Year: 2003
  4. Flavoproteins in Oxidative Protein Folding
    Colin Thorpe; Fiscal Year: 2007
  5. FLAVOPROTEINS IN FATTY ACID METABOLISM
    Colin Thorpe; Fiscal Year: 1991
  6. FLAVOPROTEINS IN FATTY ACID METABOLISM
    Colin Thorpe; Fiscal Year: 1993
  7. FLAVOPROTEINS IN FATTY ACID METABOLISM
    Colin Thorpe; Fiscal Year: 1980
  8. Flavoproteins in Oxidative Protein Folding
    Colin Thorpe; Fiscal Year: 2010

Collaborators

  • Deborah Fass
  • Shi Bai
  • Sharon Rozovsky
  • Joan Burnside
  • R D Bach
  • Vamsi K Kodali
  • Daniel J Cline
  • Vidyadhar N Daithankar
  • Joel P Schneider
  • Pumtiwitt C Rancy
  • Danny Ramadan
  • Erin J Heckler
  • Sonali Raje
  • Stephanie A Schaefer
  • Scott R Farrell
  • Wenzhong Wang
  • Stephanie Schaefer-Ramadan
  • Aparna Sapra
  • Shawn A Gannon
  • Jennifer A Codding
  • Brian J Bahnson
  • Ming Dong
  • Stephen G Brohawn
  • Olga Dmitrenko
  • Jennifer Jaje
  • Einav Gross
  • Christopher W M Kay
  • Donald L Coppock
  • Karen L Hoober
  • Renee P Rubenstein
  • Wayne A Wilkie
  • Benjamin A Israel
  • Joliene R Trujillo
  • Tatyana Polenova
  • Sivakumar Paramasivam
  • Radhika P Nagarkar
  • Assaf Alon
  • Ian H Mather
  • Olajumoke Fadugba
  • Holly N Wolcott
  • Jakob R Winther
  • Diane Cripps
  • Austin J Yang
  • Elvira Vitu
  • Nimrod Heldman
  • Carolyn S Sevier
  • Celine Elsässer
  • Moran Bentzur
  • Chris A Kaiser
  • Robert Bittl
  • James N Psathas
  • Sarah E Redding
  • Irina Rudik Miksa
  • Nicole M Glynn

Detail Information

Publications33

  1. ncbi request reprint Generating disulfides in multicellular organisms: emerging roles for a new flavoprotein family
    Colin Thorpe
    Department of Chemistry and Biochemistry, University of Delaware, Newark, DE 19716, USA
    J Biol Chem 282:13929-33. 2007
  2. ncbi request reprint Sulfhydryl oxidases: emerging catalysts of protein disulfide bond formation in eukaryotes
    Colin Thorpe
    Department of Chemistry and Biochemistry, University of Delaware, Newark, DE 19716, USA
    Arch Biochem Biophys 405:1-12. 2002
    ..In sum, this review suggests that QSOX enzymes play a significant role in oxidative folding of a large variety of proteins in a wide range of multicellular organisms...
  3. pmc Arsenic(III) species inhibit oxidative protein folding in vitro
    Danny Ramadan
    Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware 19716, USA
    Biochemistry 48:424-32. 2009
    ..In vivo, the propensity of As(III) species to bind to unfolded cysteine-containing proteins may contribute to oxidative and protein folding stresses that are prominent features of the cellular response to arsenic exposure...
  4. pmc Flavin-linked Erv-family sulfhydryl oxidases release superoxide anion during catalytic turnover
    Vidyadhar N Daithankar
    Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware 19716 2522, United States
    Biochemistry 51:265-72. 2012
    ..Aspects of the mechanism and possible physiological significance of superoxide release from these Erv-domain flavoproteins are discussed...
  5. pmc Human quiescin-sulfhydryl oxidase, QSOX1: probing internal redox steps by mutagenesis
    Erin J Heckler
    Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware 19716, USA
    Biochemistry 47:4955-63. 2008
    ....
  6. pmc A novel disulfide-rich protein motif from avian eggshell membranes
    Vamsi K Kodali
    Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware, United States of America
    PLoS ONE 6:e18187. 2011
    ..Structure is completely lost on reduction of the 8 disulfide bonds of this protein fragment. Finally, solid state NMR spectra suggest a surprising degree of order in intact ESM fibers...
  7. pmc (77)Se enrichment of proteins expands the biological NMR toolbox
    Stephanie A Schaefer
    136 Brown Laboratory, Department of Chemistry and Biochemistry, University of Delaware, Newark, DE 19716, USA
    J Mol Biol 425:222-31. 2013
    ..Hence, (77)Se NMR spectroscopy can be used to report the local electronic environment of reactive and structural sulfur sites, as well as changes taking place in those locations during catalysis...
  8. pmc Protein substrate discrimination in the quiescin sulfhydryl oxidase (QSOX) family
    Jennifer A Codding
    Department of Chemistry and Biochemistry, University of Delaware, Newark, DE 19716 2522, USA
    Biochemistry 51:4226-35. 2012
    ..These aspects of protein substrate discrimination by QSOX family members are rationalized in terms of the stringent steric requirements for disulfide exchange reactions...
  9. pmc Quiescin sulfhydryl oxidase from Trypanosoma brucei: catalytic activity and mechanism of a QSOX family member with a single thioredoxin domain
    Vamsi K Kodali
    Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware 19716, USA
    Biochemistry 49:2075-85. 2010
    ..In sum, the single-Trx domain QSOX studied here shows a striking similarity in enzymatic behavior to its double-Trx metazoan counterparts...
  10. pmc Augmenter of liver regeneration: substrate specificity of a flavin-dependent oxidoreductase from the mitochondrial intermembrane space
    Vidyadhar N Daithankar
    Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware 19716, USA
    Biochemistry 48:4828-37. 2009
    ..Thus, catalysis involves a flow of reducing equivalents from the reduced CxC motif of Mia40 to distal and then proximal CxxC motifs of lfALR to the flavin ring and, finally, to cytochrome c or molecular oxygen...
  11. ncbi request reprint Effects of As(III) binding on beta-hairpin structure
    Danny Ramadan
    Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware 19716 2522, USA
    J Am Chem Soc 129:2981-8. 2007
    ....
  12. ncbi request reprint New water-soluble phosphines as reductants of peptide and protein disulfide bonds: reactivity and membrane permeability
    Daniel J Cline
    Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware 19716, USA
    Biochemistry 43:15195-203. 2004
    ..In sum, these data suggest that there is considerable scope for the synthesis of phosphine analogues tailored for specific applications in biological systems...
  13. doi request reprint Human augmenter of liver regeneration: probing the catalytic mechanism of a flavin-dependent sulfhydryl oxidase
    Stephanie Schaefer-Ramadan
    Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware 19716 2522, United States
    Biochemistry 52:8323-32. 2013
    ....
  14. ncbi request reprint Structure-based design of a fluorimetric redox active peptide probe
    Daniel J Cline
    Department of Chemistry and Biochemistry, University of Delaware, Newark, DE 19716 2522, USA
    Anal Biochem 325:144-50. 2004
    ..The reduced peptide is an excellent substrate of the enzyme quiescin-sulfhydryl oxidase and may find utility in the characterization of other disulfide oxidoreductases...
  15. pmc Oxidative protein folding in vitro: a study of the cooperation between quiescin-sulfhydryl oxidase and protein disulfide isomerase
    Pumtiwitt C Rancy
    Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware 19716, USA
    Biochemistry 47:12047-56. 2008
    ..These in vitro experiments are discussed in terms of current models for oxidative folding in the endoplasmic reticulum...
  16. ncbi request reprint Avian sulfhydryl oxidase is not a metalloenzyme: adventitious binding of divalent metal ions to the enzyme
    Stephen G Brohawn
    Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware 19716, USA
    Biochemistry 42:11074-82. 2003
    ..The redox-modulated binding of zinc to QSOX is considered in light of evidence for a role of zinc-thiolate interactions in redox signaling and zinc mobilization...
  17. pmc Oxidative protein folding and the Quiescin-sulfhydryl oxidase family of flavoproteins
    Vamsi K Kodali
    Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware 19711, USA
    Antioxid Redox Signal 13:1217-30. 2010
    ..Finally, limitations of our current understanding of disulfide generation in metazoans are identified and questions posed for the future...
  18. ncbi request reprint Mechanism of thiolate-disulfide interchange reactions in biochemistry
    Robert D Bach
    Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware 19716, USA
    J Org Chem 73:12-21. 2008
    ..7 kcal/mol below isolated reactants. A well depth of this magnitude should provide a sufficient lifetime of the intermediate to accommodate the requisite conformational adjustments that accompanies formation of the new disulfide bond...
  19. pmc Mechanism of SN2 disulfide bond cleavage by phosphorus nucleophiles. Implications for biochemical disulfide reducing agents
    Olga Dmitrenko
    Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware 19716, USA
    J Org Chem 72:8298-307. 2007
    ....
  20. pmc Erv2p: characterization of the redox behavior of a yeast sulfhydryl oxidase
    Wenzhong Wang
    Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware 19716, USA
    Biochemistry 46:3246-54. 2007
    ..This comparison of the redox properties between members of the ERV/ALR family of sulfhydryl oxidases provides insights into their likely roles in oxidative protein folding...
  21. ncbi request reprint Inter-domain redox communication in flavoenzymes of the quiescin/sulfhydryl oxidase family: role of a thioredoxin domain in disulfide bond formation
    Sonali Raje
    Department of Chemistry and Biochemistry, University of Delaware, Newark 19716, USA
    Biochemistry 42:4560-8. 2003
    ..The ancient fusion of thioredoxin domains to a catalytically more limited ERV domain has produced an efficient catalyst for the direct introduction of disulfide bonds into a wide range of proteins and peptides in multicellular organisms...
  22. ncbi request reprint Augmenter of liver regeneration: a flavin-dependent sulfhydryl oxidase with cytochrome c reductase activity
    Scott R Farrell
    Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware 19716, USA
    Biochemistry 44:1532-41. 2005
    ..These data suggest that this poorly understood flavoenzyme may not function as a sulfhydryl oxidase within the mitochondrial intermembrane space but may communicate with the respiratory chain via the mediation of cytochrome c...
  23. pmc A flavin-dependent sulfhydryl oxidase in bovine milk
    Jennifer Jaje
    Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware 19716, USA
    Biochemistry 46:13031-40. 2007
    ..Bovine milk QSOX1 is highly active toward reduced RNase and with the model substrate dithiothreitol. The significance of these new findings is discussed in relation to the earlier reports of metal-dependent sulfhydryl oxidases...
  24. ncbi request reprint A continuous fluorescence assay for sulfhydryl oxidase
    Sonali Raje
    Department of Chemistry and Biochemistry, University of Delaware, Newark, DE 19716, USA
    Anal Biochem 307:266-72. 2002
    ..Finally, the assay is used to show that there is sulfhydryl oxidase activity in a number of secretory fluids including human tears...
  25. ncbi request reprint General method for facile intramolecular disulfide formation in synthetic peptides
    Daniel J Cline
    Department of Chemistry and Biochemistry, University of Delaware, Newark, DE 19716 2522, USA
    Anal Biochem 335:168-70. 2004
  26. pmc Structure of the human sulfhydryl oxidase augmenter of liver regeneration and characterization of a human mutation causing an autosomal recessive myopathy
    Vidyadhar N Daithankar
    Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware 19716, USA
    Biochemistry 49:6737-45. 2010
    ..In sum, these in vitro data document the major impact of the seemingly conservative R194H mutation on the stability of dimeric ALR and complement the in vivo observations of Di Fonzo et al...
  27. pmc An arsenical-maleimide for the generation of new targeted biochemical reagents
    Aparna Sapra
    Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware 19716, USA
    J Am Chem Soc 135:2415-8. 2013
    ..As-Mal will serve as a facile reagent for the incorporation of As(III) species into a wide range of thiol-containing proteins, biomaterials, and surfaces...
  28. pmc Generating disulfides with the Quiescin-sulfhydryl oxidases
    Erin J Heckler
    Department of Chemistry and Biochemistry, University of Delaware, Newark, DE 19711, USA
    Biochim Biophys Acta 1783:567-77. 2008
    ..Finally, this review identifies important unresolved questions concerning this ancient family of sulfhydryl oxidases...
  29. ncbi request reprint Flavin-dependent sulfhydryl oxidases in protein disulfide bond formation
    Karen L Hoober
    Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware 19716, USA
    Methods Enzymol 348:30-4. 2002
  30. ncbi request reprint Effects of As(III) binding on alpha-helical structure
    Daniel J Cline
    Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware 19716 2522, USA
    J Am Chem Soc 125:2923-9. 2003
    ..These data demonstrate that As(III) binding can be a significant modulator of helical secondary structure...
  31. pmc Generating disulfides enzymatically: reaction products and electron acceptors of the endoplasmic reticulum thiol oxidase Ero1p
    Einav Gross
    Department of Structural Biology, The Weizmann Institute of Science, Rehovot 76100, Israel
    Proc Natl Acad Sci U S A 103:299-304. 2006
    ..These findings provide insight into mechanisms for regenerating oxidized Ero1p and maintaining disulfide bond formation under anaerobic conditions in the endoplasmic reticulum...
  32. ncbi request reprint Multidomain flavin-dependent sulfhydryl oxidases
    Donald L Coppock
    Coriell Institute for Medical Research, Camden, New Jersey, USA
    Antioxid Redox Signal 8:300-11. 2006
    ..Finally, the structure and mechanism of QSOX proteins is compared to their smaller stand-alone cousins: yeast ERV1p and ERV2p, the mammalian augmenter of liver regeneration (ALR), and the viral ALR homologs...
  33. ncbi request reprint Determination of the distance between the two neutral flavin radicals in augmenter of liver regeneration by pulsed ELDOR
    Christopher W M Kay
    Institut fur Experimentalphysik, Fachbereich Physik, Freie Universitat Berlin, 14195 Berlin, Germany
    J Am Chem Soc 128:76-7. 2006
    ..The experiments also demonstrate that neutral flavin radicals have the appropriate properties to be used as intrinsic spin labels for distance determinations in proteins...

Research Grants33

  1. Flavoproteins in Oxidative Protein Folding
    Colin Thorpe; Fiscal Year: 2010
    ..Some of these proteins are tissue growth factors that are over-expressed in prostrate and bone cancer. A better understanding of the mechanism of these important proteins may help in the design of chemotherapeutic agents. ..
  2. FLAVOPROTEINS IN FATTY ACID METABOLISM
    Colin Thorpe; Fiscal Year: 1999
    ..Finally, the ultimate oxidant for disulfide bond formation in secreted proteins is still unclear. A new flavoenzyme oxidase isolated from egg white will be characterized and its potential role in disulfide generation will be studied. ..
  3. FLAVOPROTEINS IN FATTY ACID METABOLISM
    Colin Thorpe; Fiscal Year: 2003
    ..abstract_text> ..
  4. Flavoproteins in Oxidative Protein Folding
    Colin Thorpe; Fiscal Year: 2007
    ..The fifth and sixth aims extend this work to the enzyme's smaller, stand-alone, flavoprotein cousins: human recombinant ALR (augmenter of liver regeneration) and Erv2p (the closest functional counterpart of QSOX in yeast). ..
  5. FLAVOPROTEINS IN FATTY ACID METABOLISM
    Colin Thorpe; Fiscal Year: 1991
    ..The stable covalent flavin adduct formed upon inactivation of the reduced medium chain acyl-CoA dehydrogenase with 2-octynoyl-CoA will be characterized...
  6. FLAVOPROTEINS IN FATTY ACID METABOLISM
    Colin Thorpe; Fiscal Year: 1993
    ....
  7. FLAVOPROTEINS IN FATTY ACID METABOLISM
    Colin Thorpe; Fiscal Year: 1980
    ..Efforts will continue to utilize active site directed and suicide substrates in the exploration of the mode of substrate dehydrogenation. ..
  8. Flavoproteins in Oxidative Protein Folding
    Colin Thorpe; Fiscal Year: 2010
    ..Some of these proteins are tissue growth factors that are over-expressed in prostrate and bone cancer. A better understanding of the mechanism of these important proteins may help in the design of chemotherapeutic agents. ..