Research Topics
| N L ThompsonSummaryAffiliation: University of North Carolina Country: USA Publications
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Detail Information
Publications
Recent advances in fluorescence correlation spectroscopyNancy L Thompson
Department of Chemistry, Campus Box 3290, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 3290, USA
Curr Opin Struct Biol 12:634-41. 2002....- Detecting microdomains in intact cell membranesB Christoffer Lagerholm
Department of Cell and Developmental Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
Annu Rev Phys Chem 56:309-36. 2005..Experimental results are highlighted and the strengths and limitations of different techniques for microdomain identification and characterization are assessed... - Total internal reflection with fluorescence correlation spectroscopyNancy L Thompson
Department of Chemistry, University of North Carolina at Chapel Hill, North Carolina 27599, USA
Nat Protoc 2:878-90. 2007.... 
Measuring surface binding thermodynamics and kinetics by using total internal reflection with fluorescence correlation spectroscopy: practical considerationsNancy L Thompson
Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
J Phys Chem B 115:120-31. 2011....- Total internal reflection fluorescence: applications in cellular biophysicsN L Thompson
Department of Chemistry, Campus Box 3290, University of North Carolina, Chapel Hill, NC 27599 3290, USA
Curr Opin Biotechnol 8:58-64. 1997..These studies are providing enhanced understanding of cellular function. Several recent developments in total internal reflection fluorescence methodology from other fields are likely to find future application in cellular biophysics... - Equilibrium, kinetics, diffusion and self-association of proteins at membrane surfaces: measurement by total internal reflection fluorescence microscopyN L Thompson
Department of Chemistry, University of North Carolina, Chapel Hill 27599 3290, USA
Photochem Photobiol 65:39-46. 1997..Fluorescence correlation spectroscopy provides information about the self-association (e.g. dimerization) of membrane-associated proteins... - Total internal reflection with fluorescence correlation spectroscopy: Applications to substrate-supported planar membranesNancy L Thompson
Department of Chemistry, University of North Carolina at Chapel Hill, 27599 3290, USA
J Struct Biol 168:95-106. 2009..Methods related, but not technically equivalent, to TIR-FCS are also summarized. Future directions for TIR-FCS are outlined... - Total internal reflection with fluorescence correlation spectroscopy: combined surface reaction and solution diffusionT E Starr
Department of Chemistry, University of North Carolina, Chapel Hill 27599-3290, USA
Biophys J 80:1575-84. 2001..Both general and approximate expressions are presented... - Lipid rafts reconstituted in model membranesC Dietrich
Department of Cell Biology and Anatomy, University of North Carolina at Chapel Hill, 27599, USA
Biophys J 80:1417-28. 2001..Overall, the notion that in biomembranes selected lipids could laterally aggregate to form more ordered, detergent-resistant lipid rafts into which glycosphingolipids partition is strongly supported by this study... - Binding of a monoclonal antibody and its Fab fragment to supported phospholipid monolayers measured by total internal reflection fluorescence microscopyM L Pisarchick
Department of Chemistry, University of North Carolina, Chapel Hill 27599 3290
Biophys J 58:1235-49. 1990..Data analysis with simple theoretical models indicated that, at most antibody surface densities, 50-90% of membrane-associated intact antibodies were attached to the surface by two antigen binding sites... - Slow rotational mobilities of antibodies and lipids associated with substrate-supported phospholipid monolayers as measured by polarized fluorescence photobleaching recoveryM M Timbs
Department of Chemistry, University of North Carolina, Chapel Hill 27599 3290
Biophys J 58:413-28. 1990..No difference in fluorescence recovery and decay curves was measured for dil in DSPC monolayers, DSPC/DNP-DOPE monolayers, and DSPC/DNP-DOPE monolayers treated with unlabeled ANO2 antibodies... - Ligand-receptor kinetics measured by total internal reflection with fluorescence correlation spectroscopyAlena M Lieto
Department of Physics and Astronomy, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA
Biophys J 85:3294-302. 2003..The dissociation rate constant and rate of diffusion through the evanescent field agree with previous results, and all measured parameters were independent of the incident intensity... - Total internal reflection with fluorescence correlation spectroscopy: nonfluorescent competitorsAlena M Lieto
Department of Physics and Astronomy, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
Biophys J 87:1268-78. 2004..Both general and approximate expressions are presented... - Size dependence of protein diffusion very close to membrane surfaces: measurement by total internal reflection with fluorescence correlation spectroscopyJamie K Pero
Department of Chemistry, Campus Box 3290, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599-3290, USA
J Phys Chem B 110:10910-8. 2006..The TIR-FCS data are consistent with predictions derived from hydrodynamic theory. This work illustrates one factor that could contribute to previously observed nonideal ligand-receptor kinetics at model and natural cell membranes... - Ligand binding by estrogen receptor beta attached to nanospheres measured by fluorescence correlation spectroscopyNoah W Allen
Department of Chemistry, University of North Carolina at Asheville, 28804 8511, USA
Cytometry A 69:524-32. 2006..The advantage of using FCS with the ERbeta-LBD: bead methodology is the ability to obtain reliable and reproducible data in a short time frame... - Partitioning of Thy-1, GM1, and cross-linked phospholipid analogs into lipid rafts reconstituted in supported model membrane monolayersC Dietrich
Department of Cell and Developmental Biology, University of North Carolina, Chapel Hill, NC 27599, USA
Proc Natl Acad Sci U S A 98:10642-7. 2001..This result demonstrates that cross-linking, a process known to be important for certain cell-signaling processes, can selectively translocate molecules to liquid-ordered domains... - Fluorescence pattern photobleaching recovery for samples with multi-component diffusionTammy E Starr
Department of Chemistry, Campus Box 3290, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-3290, USA
Biophys Chem 97:29-44. 2002..The new theoretical formalism has been demonstrated on data for the translational mobility of a fluorescent lipid probe in phospholipid bilayers deposited on planar-fused silica substrates... - Binding of NAP-22, a calmodulin-binding neuronal protein, to raft-like domains in model membranesTapan K Khan
Department of Cell and Developmental Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
Biochemistry 42:4780-6. 2003..This study suggests that NAP-22 binding may be employed to image cholesterol-rich regions, such as caveolae/rafts, on the plasma membrane of cells, and preliminary efforts in that direction are presented... - Quantifying green fluorescent protein diffusion in Escherichia coli by using continuous photobleaching with evanescent illuminationKristin M Slade
Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 3290, USA
J Phys Chem B 113:4837-45. 2009..We present the theoretical basis for the technique and demonstrate its applicability by measuring the diffusion coefficient, 6.3 +/- 1.1 microm(2)/s, of green fluorescent protein in Escherichia coli cells... 
Distribution and lateral mobility of DC-SIGN on immature dendritic cells--implications for pathogen uptakeAaron K Neumann
Department of Cell and Developmental Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
J Cell Sci 121:634-43. 2008..Immature dendritic cells may acquire and internalize HIV and other pathogens by this process...- Slow conformational dynamics at C2'-endo nucleotides in RNACostin M Gherghe
Department of Chemistry, University of North Carolina, Chapel Hill, North Carolina 27599 3290, USA
J Am Chem Soc 130:8884-5. 2008..Due to their distinctive local dynamics, C2'-endo nucleotides have the potential to function as rate-determining molecular switches and are likely to play central, currently unexplored, roles in RNA folding and function... - Effects of recombinant protein expression on green fluorescent protein diffusion in Escherichia coliKristin M Slade
Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 3290, USA
Biochemistry 48:5083-9. 2009..We conclude that expression of these soluble proteins has little to no effect on the diffusion of GFP. These results have implications for the utility of in-cell nuclear magnetic resonance spectroscopy...