N L Thompson

Summary

Affiliation: University of North Carolina
Country: USA

Publications

  1. ncbi request reprint Recent advances in fluorescence correlation spectroscopy
    Nancy L Thompson
    Department of Chemistry, Campus Box 3290, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 3290, USA
    Curr Opin Struct Biol 12:634-41. 2002
  2. ncbi request reprint Detecting microdomains in intact cell membranes
    B Christoffer Lagerholm
    Department of Cell and Developmental Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Annu Rev Phys Chem 56:309-36. 2005
  3. ncbi request reprint Total internal reflection with fluorescence correlation spectroscopy
    Nancy L Thompson
    Department of Chemistry, University of North Carolina at Chapel Hill, North Carolina 27599, USA
    Nat Protoc 2:878-90. 2007
  4. pmc Measuring surface binding thermodynamics and kinetics by using total internal reflection with fluorescence correlation spectroscopy: practical considerations
    Nancy L Thompson
    Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    J Phys Chem B 115:120-31. 2011
  5. ncbi request reprint Total internal reflection fluorescence: applications in cellular biophysics
    N L Thompson
    Department of Chemistry, Campus Box 3290, University of North Carolina, Chapel Hill, NC 27599 3290, USA
    Curr Opin Biotechnol 8:58-64. 1997
  6. ncbi request reprint Equilibrium, kinetics, diffusion and self-association of proteins at membrane surfaces: measurement by total internal reflection fluorescence microscopy
    N L Thompson
    Department of Chemistry, University of North Carolina, Chapel Hill 27599 3290, USA
    Photochem Photobiol 65:39-46. 1997
  7. pmc Total internal reflection with fluorescence correlation spectroscopy: Applications to substrate-supported planar membranes
    Nancy L Thompson
    Department of Chemistry, University of North Carolina at Chapel Hill, 27599 3290, USA
    J Struct Biol 168:95-106. 2009
  8. pmc Total internal reflection with fluorescence correlation spectroscopy: combined surface reaction and solution diffusion
    T E Starr
    Department of Chemistry, University of North Carolina, Chapel Hill 27599-3290, USA
    Biophys J 80:1575-84. 2001
  9. pmc Lipid rafts reconstituted in model membranes
    C Dietrich
    Department of Cell Biology and Anatomy, University of North Carolina at Chapel Hill, 27599, USA
    Biophys J 80:1417-28. 2001
  10. pmc Binding of a monoclonal antibody and its Fab fragment to supported phospholipid monolayers measured by total internal reflection fluorescence microscopy
    M L Pisarchick
    Department of Chemistry, University of North Carolina, Chapel Hill 27599 3290
    Biophys J 58:1235-49. 1990

Collaborators

Detail Information

Publications22

  1. ncbi request reprint Recent advances in fluorescence correlation spectroscopy
    Nancy L Thompson
    Department of Chemistry, Campus Box 3290, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 3290, USA
    Curr Opin Struct Biol 12:634-41. 2002
    ....
  2. ncbi request reprint Detecting microdomains in intact cell membranes
    B Christoffer Lagerholm
    Department of Cell and Developmental Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Annu Rev Phys Chem 56:309-36. 2005
    ..Experimental results are highlighted and the strengths and limitations of different techniques for microdomain identification and characterization are assessed...
  3. ncbi request reprint Total internal reflection with fluorescence correlation spectroscopy
    Nancy L Thompson
    Department of Chemistry, University of North Carolina at Chapel Hill, North Carolina 27599, USA
    Nat Protoc 2:878-90. 2007
    ....
  4. pmc Measuring surface binding thermodynamics and kinetics by using total internal reflection with fluorescence correlation spectroscopy: practical considerations
    Nancy L Thompson
    Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    J Phys Chem B 115:120-31. 2011
    ....
  5. ncbi request reprint Total internal reflection fluorescence: applications in cellular biophysics
    N L Thompson
    Department of Chemistry, Campus Box 3290, University of North Carolina, Chapel Hill, NC 27599 3290, USA
    Curr Opin Biotechnol 8:58-64. 1997
    ..These studies are providing enhanced understanding of cellular function. Several recent developments in total internal reflection fluorescence methodology from other fields are likely to find future application in cellular biophysics...
  6. ncbi request reprint Equilibrium, kinetics, diffusion and self-association of proteins at membrane surfaces: measurement by total internal reflection fluorescence microscopy
    N L Thompson
    Department of Chemistry, University of North Carolina, Chapel Hill 27599 3290, USA
    Photochem Photobiol 65:39-46. 1997
    ..Fluorescence correlation spectroscopy provides information about the self-association (e.g. dimerization) of membrane-associated proteins...
  7. pmc Total internal reflection with fluorescence correlation spectroscopy: Applications to substrate-supported planar membranes
    Nancy L Thompson
    Department of Chemistry, University of North Carolina at Chapel Hill, 27599 3290, USA
    J Struct Biol 168:95-106. 2009
    ..Methods related, but not technically equivalent, to TIR-FCS are also summarized. Future directions for TIR-FCS are outlined...
  8. pmc Total internal reflection with fluorescence correlation spectroscopy: combined surface reaction and solution diffusion
    T E Starr
    Department of Chemistry, University of North Carolina, Chapel Hill 27599-3290, USA
    Biophys J 80:1575-84. 2001
    ..Both general and approximate expressions are presented...
  9. pmc Lipid rafts reconstituted in model membranes
    C Dietrich
    Department of Cell Biology and Anatomy, University of North Carolina at Chapel Hill, 27599, USA
    Biophys J 80:1417-28. 2001
    ..Overall, the notion that in biomembranes selected lipids could laterally aggregate to form more ordered, detergent-resistant lipid rafts into which glycosphingolipids partition is strongly supported by this study...
  10. pmc Binding of a monoclonal antibody and its Fab fragment to supported phospholipid monolayers measured by total internal reflection fluorescence microscopy
    M L Pisarchick
    Department of Chemistry, University of North Carolina, Chapel Hill 27599 3290
    Biophys J 58:1235-49. 1990
    ..Data analysis with simple theoretical models indicated that, at most antibody surface densities, 50-90% of membrane-associated intact antibodies were attached to the surface by two antigen binding sites...
  11. pmc Slow rotational mobilities of antibodies and lipids associated with substrate-supported phospholipid monolayers as measured by polarized fluorescence photobleaching recovery
    M M Timbs
    Department of Chemistry, University of North Carolina, Chapel Hill 27599 3290
    Biophys J 58:413-28. 1990
    ..No difference in fluorescence recovery and decay curves was measured for dil in DSPC monolayers, DSPC/DNP-DOPE monolayers, and DSPC/DNP-DOPE monolayers treated with unlabeled ANO2 antibodies...
  12. pmc Ligand-receptor kinetics measured by total internal reflection with fluorescence correlation spectroscopy
    Alena M Lieto
    Department of Physics and Astronomy, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA
    Biophys J 85:3294-302. 2003
    ..The dissociation rate constant and rate of diffusion through the evanescent field agree with previous results, and all measured parameters were independent of the incident intensity...
  13. pmc Total internal reflection with fluorescence correlation spectroscopy: nonfluorescent competitors
    Alena M Lieto
    Department of Physics and Astronomy, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Biophys J 87:1268-78. 2004
    ..Both general and approximate expressions are presented...
  14. ncbi request reprint Size dependence of protein diffusion very close to membrane surfaces: measurement by total internal reflection with fluorescence correlation spectroscopy
    Jamie K Pero
    Department of Chemistry, Campus Box 3290, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 3290, USA
    J Phys Chem B 110:10910-8. 2006
    ..The TIR-FCS data are consistent with predictions derived from hydrodynamic theory. This work illustrates one factor that could contribute to previously observed nonideal ligand-receptor kinetics at model and natural cell membranes...
  15. ncbi request reprint Ligand binding by estrogen receptor beta attached to nanospheres measured by fluorescence correlation spectroscopy
    Noah W Allen
    Department of Chemistry, University of North Carolina at Asheville, 28804 8511, USA
    Cytometry A 69:524-32. 2006
    ..The advantage of using FCS with the ERbeta-LBD: bead methodology is the ability to obtain reliable and reproducible data in a short time frame...
  16. pmc Partitioning of Thy-1, GM1, and cross-linked phospholipid analogs into lipid rafts reconstituted in supported model membrane monolayers
    C Dietrich
    Department of Cell and Developmental Biology, University of North Carolina, Chapel Hill, NC 27599, USA
    Proc Natl Acad Sci U S A 98:10642-7. 2001
    ..This result demonstrates that cross-linking, a process known to be important for certain cell-signaling processes, can selectively translocate molecules to liquid-ordered domains...
  17. ncbi request reprint Fluorescence pattern photobleaching recovery for samples with multi-component diffusion
    Tammy E Starr
    Department of Chemistry, Campus Box 3290, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 3290, USA
    Biophys Chem 97:29-44. 2002
    ..The new theoretical formalism has been demonstrated on data for the translational mobility of a fluorescent lipid probe in phospholipid bilayers deposited on planar-fused silica substrates...
  18. ncbi request reprint Binding of NAP-22, a calmodulin-binding neuronal protein, to raft-like domains in model membranes
    Tapan K Khan
    Department of Cell and Developmental Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Biochemistry 42:4780-6. 2003
    ..This study suggests that NAP-22 binding may be employed to image cholesterol-rich regions, such as caveolae/rafts, on the plasma membrane of cells, and preliminary efforts in that direction are presented...
  19. pmc Quantifying green fluorescent protein diffusion in Escherichia coli by using continuous photobleaching with evanescent illumination
    Kristin M Slade
    Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 3290, USA
    J Phys Chem B 113:4837-45. 2009
    ..We present the theoretical basis for the technique and demonstrate its applicability by measuring the diffusion coefficient, 6.3 +/- 1.1 microm(2)/s, of green fluorescent protein in Escherichia coli cells...
  20. doi request reprint Distribution and lateral mobility of DC-SIGN on immature dendritic cells--implications for pathogen uptake
    Aaron K Neumann
    Department of Cell and Developmental Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    J Cell Sci 121:634-43. 2008
    ..Immature dendritic cells may acquire and internalize HIV and other pathogens by this process...
  21. doi request reprint Slow conformational dynamics at C2'-endo nucleotides in RNA
    Costin M Gherghe
    Department of Chemistry, University of North Carolina, Chapel Hill, North Carolina 27599 3290, USA
    J Am Chem Soc 130:8884-5. 2008
    ..Due to their distinctive local dynamics, C2'-endo nucleotides have the potential to function as rate-determining molecular switches and are likely to play central, currently unexplored, roles in RNA folding and function...
  22. doi request reprint Effects of recombinant protein expression on green fluorescent protein diffusion in Escherichia coli
    Kristin M Slade
    Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 3290, USA
    Biochemistry 48:5083-9. 2009
    ..We conclude that expression of these soluble proteins has little to no effect on the diffusion of GFP. These results have implications for the utility of in-cell nuclear magnetic resonance spectroscopy...