E B Thompson

Summary

Affiliation: University of Texas Medical Branch
Country: USA

Publications

  1. ncbi request reprint Gene regulation by the glucocorticoid receptor: structure:function relationship
    Raj Kumar
    Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, 301 University Boulevard, Galveston, TX 77555 1068, USA
    J Steroid Biochem Mol Biol 94:383-94. 2005
  2. pmc Gene expression profiling of leukemic cells and primary thymocytes predicts a signature for apoptotic sensitivity to glucocorticoids
    Aaron L Miller
    Department of Biochemistry and Molecular Biology, The University of Texas Medical Branch, Galveston, TX, USA
    Cancer Cell Int 7:18. 2007
  3. pmc Pathway interactions between MAPKs, mTOR, PKA, and the glucocorticoid receptor in lymphoid cells
    Aaron L Miller
    Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, 301 University Boulevard, Galveston, Texas 77555 1068, USA
    Cancer Cell Int 7:3. 2007
  4. ncbi request reprint Stepping stones in the path of glucocorticoid-driven apoptosis of lymphoid cells
    E Brad Thompson
    Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, Texas 77555 1068, USA
    Acta Biochim Biophys Sin (Shanghai) 40:595-600. 2008
  5. ncbi request reprint Identification of genes leading to glucocorticoid-induced leukemic cell death
    E B Thompson
    The University of Texas Medical Branch, Department of Human Biological Chemistry and Genetics, Galveston, Texas 77555 1068, USA
    Lipids 39:821-5. 2004
  6. ncbi request reprint Regulation of a distinctive set of genes in glucocorticoid-evoked apoptosis in CEM human lymphoid cells
    E Brad Thompson
    Department of Human Biological Chemistry and Genetics, The University of Texas Medical Branch, Galveston, Texas 77555 0645, USA
    Recent Prog Horm Res 58:175-97. 2003
  7. ncbi request reprint The many roles of c-Myc in apoptosis
    E B Thompson
    Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston 77555 0645, USA
    Annu Rev Physiol 60:575-600. 1998
  8. ncbi request reprint DNA binding of nuclear hormone receptors influences their structure and function
    E Brad Thompson
    The University of Texas Medical Branch, Department of Human Biological Chemistry and Genetics, Galveston, TX 77555 0645, USA
    Biochem Biophys Res Commun 306:1-4. 2003
  9. pmc Glucocorticoids, oxysterols, and cAMP with glucocorticoids each cause apoptosis of CEM cells and suppress c-myc
    E B Thompson
    The Department of Human Biological Chemistry and Genetics, The University of Texas Medical Branch, Galveston 77555 0645, USA
    J Steroid Biochem Mol Biol 69:453-61. 1999
  10. ncbi request reprint Involvement of caspases in 4-hydroxy-alkenal-induced apoptosis in human leukemic cells
    W Zhang
    Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston, TX 77555-0647, USA
    Free Radic Biol Med 30:699-706. 2001

Collaborators

Detail Information

Publications41

  1. ncbi request reprint Gene regulation by the glucocorticoid receptor: structure:function relationship
    Raj Kumar
    Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, 301 University Boulevard, Galveston, TX 77555 1068, USA
    J Steroid Biochem Mol Biol 94:383-94. 2005
    ..Because of the close similarities in modular organization among the members of the nuclear hormone receptors, the principles discussed here for the GR should be applicable to many other receptors in the family as well...
  2. pmc Gene expression profiling of leukemic cells and primary thymocytes predicts a signature for apoptotic sensitivity to glucocorticoids
    Aaron L Miller
    Department of Biochemistry and Molecular Biology, The University of Texas Medical Branch, Galveston, TX, USA
    Cancer Cell Int 7:18. 2007
    ..Kasumi-1 cells gave an example of the rare Dex-sensitive acute myeloblastic leukemia (AML). To test the generality of the correlations in malignant cell gene sets, we compared with GC effects on mouse non-transformed thymocytes...
  3. pmc Pathway interactions between MAPKs, mTOR, PKA, and the glucocorticoid receptor in lymphoid cells
    Aaron L Miller
    Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, 301 University Boulevard, Galveston, Texas 77555 1068, USA
    Cancer Cell Int 7:3. 2007
    ....
  4. ncbi request reprint Stepping stones in the path of glucocorticoid-driven apoptosis of lymphoid cells
    E Brad Thompson
    Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, Texas 77555 1068, USA
    Acta Biochim Biophys Sin (Shanghai) 40:595-600. 2008
    ..A central core of networked genes may be found under GC control in many types of malignant, GC-sensitive cells. The partial core list identified should be tested in clinical cell samples from hematologic malignancies...
  5. ncbi request reprint Identification of genes leading to glucocorticoid-induced leukemic cell death
    E B Thompson
    The University of Texas Medical Branch, Department of Human Biological Chemistry and Genetics, Galveston, Texas 77555 1068, USA
    Lipids 39:821-5. 2004
    ..The apoptosis-resistant clone C1-15 showed Dex effects on a largely different set of genes. Promoter analysis of the regulated genes suggested that primary gene targets for GC often lack a classic GC response element...
  6. ncbi request reprint Regulation of a distinctive set of genes in glucocorticoid-evoked apoptosis in CEM human lymphoid cells
    E Brad Thompson
    Department of Human Biological Chemistry and Genetics, The University of Texas Medical Branch, Galveston, Texas 77555 0645, USA
    Recent Prog Horm Res 58:175-97. 2003
    ..These data support our hypothesis and further show that the glucocorticoid-resistant clone is capable of responding to steroid but with a different set of genes. We propose that a general metabolic switch accounts for the alteration...
  7. ncbi request reprint The many roles of c-Myc in apoptosis
    E B Thompson
    Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston 77555 0645, USA
    Annu Rev Physiol 60:575-600. 1998
    ..Hypotheses of the mechanisms by which expression and repression of c-myc lead to apoptosis are discussed...
  8. ncbi request reprint DNA binding of nuclear hormone receptors influences their structure and function
    E Brad Thompson
    The University of Texas Medical Branch, Department of Human Biological Chemistry and Genetics, Galveston, TX 77555 0645, USA
    Biochem Biophys Res Commun 306:1-4. 2003
  9. pmc Glucocorticoids, oxysterols, and cAMP with glucocorticoids each cause apoptosis of CEM cells and suppress c-myc
    E B Thompson
    The Department of Human Biological Chemistry and Genetics, The University of Texas Medical Branch, Galveston 77555 0645, USA
    J Steroid Biochem Mol Biol 69:453-61. 1999
    ....
  10. ncbi request reprint Involvement of caspases in 4-hydroxy-alkenal-induced apoptosis in human leukemic cells
    W Zhang
    Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston, TX 77555-0647, USA
    Free Radic Biol Med 30:699-706. 2001
    ....
  11. pmc Constitutive expression of ectopic c-Myc delays glucocorticoid-evoked apoptosis of human leukemic CEM-C7 cells
    R D Medh
    Department of Human Biological Chemistry and Genetics, The University of Texas Medical Branch, Galveston, Texas, TX 77555 0645, USA
    Oncogene 20:4629-39. 2001
    ..Our results emphasize the importance of c-Myc suppression in GC-evoked apoptosis of CEM-C7-14 cells...
  12. ncbi request reprint Identification of the activation-labile gene: a single point mutation in the human glucocorticoid receptor presents as two distinct receptor phenotypes
    J Ashraf
    Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston 77550 0645
    Mol Endocrinol 7:631-42. 1993
    ..A single point mutation in the steroid-binding domain of the GR, therefore, may behave differently, depending on the cellular milieu in which it is expressed...
  13. ncbi request reprint Overexpression of full-length human glucocorticoid receptor in Spodoptera frugiperda cells using the baculovirus expression vector system
    G Srinivasan
    Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston 77550
    Mol Endocrinol 4:209-16. 1990
    ..By scaling up this culture we can, for the first time, obtain enough purified full-length receptor for crystallographic and functional studies which could provide new insight into exactly how hGR works...
  14. ncbi request reprint Heat shock protein is tightly associated with the recombinant human glucocorticoid receptor:glucocorticoid response element complex
    G Srinivasan
    Department of Human Biological Chemistry, University of Texas Medical Branch, Galveston 77555 0645
    Mol Endocrinol 8:189-96. 1994
    ..The in vitro activated hGR has been shown to be highly active in an in vitro transcription system. We speculate that hsp70 may influence the DNA-binding and/or transcriptional activities of hGR...
  15. pmc Effects of different osmolytes on the induced folding of the N-terminal activation domain (AF1) of the glucocorticoid receptor
    R Kumar
    Department of Internal Medicine, Division of Gastroenterology, University of Texas Medical Branch, 301 University Blvd, Galveston, TX 77555 1071, USA
    Arch Biochem Biophys 465:452-60. 2007
    ..The cell-specific functions of the GR AF1--and by extension the AF1s of other nuclear hormone receptors--may in part be affected by the presence and concentrations of particular osmolytes within a particular cellular environment...
  16. ncbi request reprint The structure of the nuclear hormone receptors
    R Kumar
    Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch at Galveston, 77555 0645, USA
    Steroids 64:310-9. 1999
    ..In this review, we summarize and discuss the current information on structures of the steroid-thyroid-retinoid receptors...
  17. ncbi request reprint Elevated expression of Bcl-2 and Bcl-x by intestinal intraepithelial lymphocytes: resistance to apoptosis by glucocorticoids and irradiation
    N Van Houten
    Department of Internal Medicine, University of Texas Medical Branch, Galveston 77555, USA
    Int Immunol 9:945-53. 1997
    ..Also, the high levels of Bcl-2 and Bcl-x in this in vivo activated population supports the in vitro correlate of protection from activation-induced cell death...
  18. pmc Intrinsic disorder as a mechanism to optimize allosteric coupling in proteins
    Vincent J Hilser
    Department of Biochemistry and Molecular Biology, and Sealy Center for Structural Biology and Molecular Biophysics, University of Texas Medical Branch, Galveston, TX 77555 1068, USA
    Proc Natl Acad Sci U S A 104:8311-5. 2007
    ....
  19. pmc Hormonal regulation of physiological cell turnover and apoptosis
    R D Medh
    Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch at Galveston 77555 0645, USA
    Cell Tissue Res 301:101-24. 2000
    ..Here we review the mechanisms by which hormonal and cytokine factors mediate cell turnover in various tissues, emphasizing common themes and tissue-specific differences...
  20. ncbi request reprint Cellular lipid peroxidation end-products induce apoptosis in human lens epithelial cells
    S Choudhary
    Department of Human Biological Chemistry and Genetics, The University of Texas Medical Branch, Galveston, TX, USA
    Free Radic Biol Med 32:360-9. 2002
    ..Taken together, the results demonstrate the apoptotic potential of the lipid peroxidation end-products and suggest that H(2)O(2)-induced apoptosis may be mediated by these end-products in the lens epithelium...
  21. ncbi request reprint Activation function 1 of glucocorticoid receptor binds TATA-binding protein in vitro and in vivo
    Alicja J Copik
    Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, 301 University Boulevard, Galveston, Texas 77555 1068, USA
    Mol Endocrinol 20:1218-30. 2006
    ..To demonstrate the functional role of the in vivo GR-TBP interaction, increased amounts of TBP expressed in vivo stimulated expression of GR-driven reporters and endogenous genes, and the effect was also specifically dependent on AF1...
  22. ncbi request reprint p38 Mitogen-activated protein kinase (MAPK) is a key mediator in glucocorticoid-induced apoptosis of lymphoid cells: correlation between p38 MAPK activation and site-specific phosphorylation of the human glucocorticoid receptor at serine 211
    Aaron L Miller
    Department of Human Biological Chemistry and Genetics, The University of Texas Medical Branch, 301 University Boulevard, Room 5 104, Medical Research Building, Route 1068, Galveston, Texas 77555 1068, USA
    Mol Endocrinol 19:1569-83. 2005
    ..Mutation of this site to alanine greatly diminished GR-driven gene transcription and apoptosis. Our results clearly demonstrate a role for p38 MAPK signaling in the pathway of GC-induced apoptosis of lymphoid cells...
  23. ncbi request reprint Overview of the structural basis for transcription regulation by nuclear hormone receptors
    Raj Kumar
    Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, 5 104 Medical Research Bldg, Galveston, TX 77555, U S A
    Essays Biochem 40:27-39. 2004
    ..However, precisely how signals from a ligand through its NHR are passed to specific genes is still unknown. Herein, we present a broad overview of current knowledge on the structure and functions of the NHRs...
  24. ncbi request reprint Optimal ligand binding by the recombinant human glucocorticoid receptor and assembly of the receptor complex with heat shock protein 90 correlate with high intracellular ATP levels in Spodoptera frugiperda cells
    G Srinivasan
    Department of Human Biological Chemistry and Genetics, The University of Texas Medical Branch, Galveston 77555 0645, U S A
    J Steroid Biochem Mol Biol 60:1-9. 1997
    ..Besides having practical consequences for the production of recombinant GR and other related proteins, our findings could ultimately have relevance in diseases such as diabetes mellitus...
  25. ncbi request reprint Cloning of a teleost fish glucocorticoid receptor shows that it contains a deoxyribonucleic acid-binding domain different from that of mammals
    B Ducouret
    Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston 77555 0645, USA
    Endocrinology 136:3774-83. 1995
    ..Computer analysis of the known sequences of GRs and MRs, however, suggests that the fish GR did not give rise to the MR of higher animals, but that both subfamilies of receptor arose from some earlier gene...
  26. ncbi request reprint The conformation of the glucocorticoid receptor af1/tau1 domain induced by osmolyte binds co-regulatory proteins
    R Kumar
    Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston, Texas 77555, USA
    J Biol Chem 276:18146-52. 2001
    ..These data indicate that TMAO induces a conformation in AF1/tau1 that is important for its interaction with certain co-regulatory proteins...
  27. ncbi request reprint Extinction of prolactin gene expression in somatic cell hybrids is correlated with the repression of the pituitary-specific trans-activator GHF-1/Pit-1
    S C Supowit
    University of Texas Medical Branch, Department of Human Biological Chemistry and Genetics, Galveston 77550
    Mol Endocrinol 6:786-92. 1992
    ..These data suggest that in pituitary x fibroblast hybrids repression of the trans-activator GHF-1/Pit-1 is a primary mechanism for the extinction of PRL and GH gene expression...
  28. ncbi request reprint Gene networks in glucocorticoid-evoked apoptosis of leukemic cells
    M Scott Webb
    Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, 301 University Boulevard, Galveston, TX 77555 0645, USA
    J Steroid Biochem Mol Biol 85:183-93. 2003
    ..A number of the genes basally higher in the resistant clone are potentially anti-apoptotic. This is consistent with our hypothesis that the resistant cells have undergone a general shift in gene expression...
  29. ncbi request reprint Comparison of two structurally diverse glucocorticoid receptor agonists: cortivazol selectively regulates a distinct set of genes separate from dexamethasone in CEM cells
    Aaron L Miller
    The University of Texas Medical Branch, Department of Biochemistry and Molecular Biology, 301 University Blvd, Galveston, TX 77555 1068, USA
    Steroids 72:673-81. 2007
    ..Many of the cortivazol specific genes are key components of various signal transduction pathways. Our data clearly show cortivazol to be a selective modulator of GR action...
  30. pmc Mutational analysis of DBD*--a unique antileukemic gene sequence
    Yan shan Ji
    Department of Human Biological Chemistry and Genetics, The University of Texas Medical Branch, Galveston, TX 77555 0645, USA
    Neoplasia 4:417-23. 2002
    ..Transfections of fragments of DBD* showed that optimal activity localized to the sequence for its C-terminal 32 amino acids...
  31. pmc TATA box binding protein induces structure in the recombinant glucocorticoid receptor AF1 domain
    Raj Kumar
    Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston, TX 77555, USA
    Proc Natl Acad Sci U S A 101:16425-30. 2004
    ..These results are in accordance with the hypothesis that an induced-fit mechanism gives structure to the GR AF1 when it encounters TBP...
  32. ncbi request reprint Transactivation functions of the N-terminal domains of nuclear hormone receptors: protein folding and coactivator interactions
    Raj Kumar
    Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston, Texas 77555, USA
    Mol Endocrinol 17:1-10. 2003
    ..We propose models for the folding of the NTD AFs and their protein-protein interactions...
  33. pmc In CEM cells the autosomal deafness gene dfna5 is regulated by glucocorticoids and forskolin
    M S Webb
    Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, 301 University Boulevard, Galveston, TX 77555 1068, USA
    J Steroid Biochem Mol Biol 107:15-21. 2007
    ..Induction of dfna5 mRNA correlates with GC-dependent apoptosis of CEM cells, though dfna5 expression alone is not sufficient for apoptosis...
  34. pmc Glucocorticoids and polyamine inhibitors synergize to kill human leukemic CEM cells
    Aaron L Miller
    Department of Human Biological Chemistry and Genetics, The University of Texas Medical Branch, Galveston 77550 0645, USA
    Neoplasia 4:68-81. 2002
    ..Both the rapidity and extent of cell kill are enhanced compared to the effects of Dex alone. These results suggest that use of such combinations in vivo may result in apoptosis of malignant cells with lower overall toxicity...
  35. pmc Protein kinase A (PKA) isoform RIIbeta mediates the synergistic killing effect of cAMP and glucocorticoid in acute lymphoblastic leukemia cells
    Zhenyu Ji
    Department of Pharmacology and Toxicology, Sealy Center for Cancer Cell Biology, School of Medicine, The University of Texas Medical Branch, Galveston, Texas 77555 1031, USA
    J Biol Chem 283:21920-5. 2008
    ....
  36. ncbi request reprint Pyridinium cationic lipids in gene delivery: an in vitro and in vivo comparison of transfection efficiency versus a tetraalkylammonium congener
    Marc A Ilies
    Department of Marine Sciences, Texas A and M University at Galveston, 5007 Avenue U, Galveston, TX 77551, USA
    Arch Biochem Biophys 435:217-26. 2005
    ....
  37. ncbi request reprint Pyridinium cationic lipids in gene delivery: a structure-activity correlation study
    Marc Antoniu Ilies
    Department of Marine Sciences, Texas A and M University at Galveston, 5007 Avenue U, Galveston, TX 77551, USA
    J Med Chem 47:3744-54. 2004
    ..Subsequent tests on other malignancies yielded similarly promising results...
  38. ncbi request reprint Induced alpha-helix structure in AF1 of the androgen receptor upon binding transcription factor TFIIF
    Raj Kumar
    Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston, Texas 77555 1068, USA
    Biochemistry 43:3008-13. 2004
    ..We further show that this induced conformation in the AR AF1 is well-suited for its interaction with SRC-1...
  39. ncbi request reprint Protein kinase A, not Epac, suppresses hedgehog activity and regulates glucocorticoid sensitivity in acute lymphoblastic leukemia cells
    Zhenyu Ji
    Department of Pharmacology and Toxicology, School of Medicine, The University of Texas Medical Branch, Galveston, TX 77555, USA
    J Biol Chem 282:37370-7. 2007
    ..These results suggest that Hh activity is critical for leukemia cell growth and survival and that the level of Hh activity is in part responsible for the synergism between cAMP and GC...
  40. doi request reprint Converting cell lines representing hematological malignancies from glucocorticoid-resistant to glucocorticoid-sensitive: signaling pathway interactions
    Anna S Garza
    The University of Texas Medical Branch, Department of Biochemistry and Molecular Biology, Galveston, TX 77555 1068, USA
    Leuk Res 33:717-27. 2009
    ..Sensitivity to GC-driven apoptosis correlated with GC-dependent increases in phosphorylated and total glucocorticoid receptor, and in increased levels of the pro-apoptotic protein Bim...
  41. pmc Gene expression profile of human lymphoid CEM cells sensitive and resistant to glucocorticoid-evoked apoptosis
    Rheem D Medh
    Department of Biology, California State University, Northridge, CA 91330 8303, USA
    Genomics 81:543-55. 2003
    ..The altered genes identified provide new foci for study of their role in cell death...