Stephen Thom

Summary

Affiliation: University of Pennsylvania
Country: USA

Publications

  1. doi request reprint Carbon monoxide transport and actions in blood and tissues
    Stephen R Thom
    Department of Emergency Medicine, Institute for Environmental Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA
    Compr Physiol 1:421-46. 2011
  2. doi request reprint Bubbles, microparticles, and neutrophil activation: changes with exercise level and breathing gas during open-water SCUBA diving
    Stephen R Thom
    Institute for Environmental Medicine, University of Pennsylvania Medical Center, Philadelphia, PA 19104, USA
    J Appl Physiol (1985) 114:1396-405. 2013
  3. doi request reprint Intramicroparticle nitrogen dioxide is a bubble nucleation site leading to decompression-induced neutrophil activation and vascular injury
    Stephen R Thom
    Institute for Environmental Medicine, Univ of Pennsylvania, Philadelphia, PA 19104 6068, USA
    J Appl Physiol (1985) 114:550-8. 2013
  4. pmc Nitric-oxide synthase-2 linkage to focal adhesion kinase in neutrophils influences enzyme activity and β2 integrin function
    Stephen R Thom
    Department of Emergency Medicine, University of Pennsylvania Medical Center and School of Biomedical Engineering, Philadelphia, Pennsylvania 19104, USA
    J Biol Chem 288:4810-8. 2013
  5. pmc Thioredoxin reductase linked to cytoskeleton by focal adhesion kinase reverses actin S-nitrosylation and restores neutrophil β(2) integrin function
    Stephen R Thom
    Institute for Environmental Medicine, University of Pennsylvania Medical Center, Philadelphia, PA 19104, USA
    J Biol Chem 287:30346-57. 2012
  6. doi request reprint Microparticle production, neutrophil activation, and intravascular bubbles following open-water SCUBA diving
    Stephen R Thom
    Institute for Environmental Medicine, Department of Emergency Medicine, University of Pennsylvania Medical Center, Philadelphia, PA, USA
    J Appl Physiol (1985) 112:1268-78. 2012
  7. ncbi request reprint Release of glutathione from erythrocytes and other markers of oxidative stress in carbon monoxide poisoning
    S R Thom
    Institute for Environmental Medicine, University of Pennsylvania, Philadelphia 19104 6068, USA
    J Appl Physiol (1985) 82:1424-32. 1997
  8. pmc Oxidative stress is fundamental to hyperbaric oxygen therapy
    Stephen R Thom
    Department of Emergency Medicine, Institute for Environmental Medicine, University of Pennsylvania Medical Center, Philadelphia, Pennsylvania 19104 6068, USA
    J Appl Physiol (1985) 106:988-95. 2009
  9. doi request reprint Plasma biomarkers in carbon monoxide poisoning
    Stephen R Thom
    Department of Emergency Medicine, University of Pennsylvania, Philadelphia, PA, USA
    Clin Toxicol (Phila) 48:47-56. 2010
  10. doi request reprint Microparticles initiate decompression-induced neutrophil activation and subsequent vascular injuries
    Stephen R Thom
    Institute for Environmental Medicine, Univ of Pennsylvania, 1 John Morgan Bldg, 3620 Hamilton Walk, Philadelphia, PA 19104 6068, USA
    J Appl Physiol (1985) 110:340-51. 2011

Research Grants

Detail Information

Publications39

  1. doi request reprint Carbon monoxide transport and actions in blood and tissues
    Stephen R Thom
    Department of Emergency Medicine, Institute for Environmental Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA
    Compr Physiol 1:421-46. 2011
    ..Because CO binds competitively to heme-containing proteins its effects depend on CO concentration relative to alternative ligands. Therefore, some discussion is devoted to how nitric oxide and hydrogen sulfide influence CO effects...
  2. doi request reprint Bubbles, microparticles, and neutrophil activation: changes with exercise level and breathing gas during open-water SCUBA diving
    Stephen R Thom
    Institute for Environmental Medicine, University of Pennsylvania Medical Center, Philadelphia, PA 19104, USA
    J Appl Physiol (1985) 114:1396-405. 2013
    ..9, P < 0.05). We conclude that postdecompression relationships among bubbles, MPs, platelet-neutrophil interactions, and neutrophil activation appear to exist, but more study is required to improve confidence in the associations...
  3. doi request reprint Intramicroparticle nitrogen dioxide is a bubble nucleation site leading to decompression-induced neutrophil activation and vascular injury
    Stephen R Thom
    Institute for Environmental Medicine, Univ of Pennsylvania, Philadelphia, PA 19104 6068, USA
    J Appl Physiol (1985) 114:550-8. 2013
    ..We conclude that nitrogen dioxide is a nascent gas nucleation site synthesized in some MPs and is responsible for initiating postdecompression inflammatory injuries...
  4. pmc Nitric-oxide synthase-2 linkage to focal adhesion kinase in neutrophils influences enzyme activity and β2 integrin function
    Stephen R Thom
    Department of Emergency Medicine, University of Pennsylvania Medical Center and School of Biomedical Engineering, Philadelphia, Pennsylvania 19104, USA
    J Biol Chem 288:4810-8. 2013
    ..We conclude that iNOS activity is increased by an FAK-mediated association with actin filaments but peak nitric oxide production is transient due to actin S-nitrosylation during exposure to hyperoxia...
  5. pmc Thioredoxin reductase linked to cytoskeleton by focal adhesion kinase reverses actin S-nitrosylation and restores neutrophil β(2) integrin function
    Stephen R Thom
    Institute for Environmental Medicine, University of Pennsylvania Medical Center, Philadelphia, PA 19104, USA
    J Biol Chem 287:30346-57. 2012
    ..Uniquely, the FAK kinase domain is the site of TrxR linkage. We conclude that through its scaffold function, FAK influences TrxR activity and actin S-nitrosylation...
  6. doi request reprint Microparticle production, neutrophil activation, and intravascular bubbles following open-water SCUBA diving
    Stephen R Thom
    Institute for Environmental Medicine, Department of Emergency Medicine, University of Pennsylvania Medical Center, Philadelphia, PA, USA
    J Appl Physiol (1985) 112:1268-78. 2012
    ..Whether there are causal relationships among bubbles, MPs, platelet-neutrophil interactions, and neutrophil activation remains obscure and requires additional study...
  7. ncbi request reprint Release of glutathione from erythrocytes and other markers of oxidative stress in carbon monoxide poisoning
    S R Thom
    Institute for Environmental Medicine, University of Pennsylvania, Philadelphia 19104 6068, USA
    J Appl Physiol (1985) 82:1424-32. 1997
    ....
  8. pmc Oxidative stress is fundamental to hyperbaric oxygen therapy
    Stephen R Thom
    Department of Emergency Medicine, Institute for Environmental Medicine, University of Pennsylvania Medical Center, Philadelphia, Pennsylvania 19104 6068, USA
    J Appl Physiol (1985) 106:988-95. 2009
    ....
  9. doi request reprint Plasma biomarkers in carbon monoxide poisoning
    Stephen R Thom
    Department of Emergency Medicine, University of Pennsylvania, Philadelphia, PA, USA
    Clin Toxicol (Phila) 48:47-56. 2010
    ..The goal was to assess whether any values differed from age- and sex-matched controls using a commercially available plasma screening package...
  10. doi request reprint Microparticles initiate decompression-induced neutrophil activation and subsequent vascular injuries
    Stephen R Thom
    Institute for Environmental Medicine, Univ of Pennsylvania, 1 John Morgan Bldg, 3620 Hamilton Walk, Philadelphia, PA 19104 6068, USA
    J Appl Physiol (1985) 110:340-51. 2011
    ..We conclude that MPs generated by decompression stresses precipitate neutrophil activation and vascular damage...
  11. pmc Hyperbaric oxygen: its mechanisms and efficacy
    Stephen R Thom
    Institute for Environmental Medicine and Department of Emergency Medicine, University of Pennsylvania Medical Center, Philadelphia, PA, USA
    Plast Reconstr Surg 127:131S-141S. 2011
    ..This article outlines therapeutic mechanisms of hyperbaric oxygen therapy and reviews data on its efficacy for clinical problems seen by plastic and reconstructive surgeons...
  12. pmc Vasculogenic stem cell mobilization and wound recruitment in diabetic patients: increased cell number and intracellular regulatory protein content associated with hyperbaric oxygen therapy
    Stephen R Thom
    Institute for Environmental Medicine, University of Pennsylvania Medical Center, Philadelphia, Pennsylvania 19104 6068, USA
    Wound Repair Regen 19:149-61. 2011
    ..We conclude that HBO(2) T stimulates vasculogenic stem cell mobilization from bone marrow of diabetics and more cells are recruited to skin wounds...
  13. pmc Neutrophil beta2 integrin inhibition by enhanced interactions of vasodilator-stimulated phosphoprotein with S-nitrosylated actin
    Stephen R Thom
    Institute for Environmental Medicine, Department of Emergency Medicine, University of Pennsylvania Medical Center, Philadelphia, Pennsylvania 19104, USA
    J Biol Chem 286:32854-65. 2011
    ....
  14. pmc Intravascular neutrophil activation due to carbon monoxide poisoning
    Stephen R Thom
    Institute for Environmental Medicine, Department of Emergency Medicine, University of Pennsylvania, 1 John Morgan Building, 3620 Hamilton Walk, Philadelphia, PA 19104 6068, USA
    Am J Respir Crit Care Med 174:1239-48. 2006
    ..We hypothesized that platelet-neutrophil interactions occur as a result of acute carbon monoxide (CO) poisoning, and subsequent neutrophil activation triggers events that cause neurologic sequelae...
  15. ncbi request reprint Hyperbaric oxygen reduces delayed immune-mediated neuropathology in experimental carbon monoxide toxicity
    Stephen R Thom
    Department of Emergency Medicine, University of Pennsylvania Medical Center, Philadelphia, 19104 6068, USA
    Toxicol Appl Pharmacol 213:152-9. 2006
    ..The results indicate that HBO(2) prevents immune-mediated delayed neurological dysfunction following CO poisoning...
  16. pmc Delayed neuropathology after carbon monoxide poisoning is immune-mediated
    Stephen R Thom
    Institute for Environmental Medicine, Department of Emergency Medicine, University of Pennsylvania Medical Center, 3620 Hamilton Walk, Philadelphia, PA 19104 6068, USA
    Proc Natl Acad Sci U S A 101:13660-5. 2004
    ..These results demonstrate that delayed CO-mediated neuropathology is linked to an adaptive immunological response to chemically modified MBP...
  17. ncbi request reprint Neuronal nitric oxide synthase and N-methyl-D-aspartate neurons in experimental carbon monoxide poisoning
    Stephen R Thom
    Institute for Environmental Medicine, University of Pennsylvania Medical Center, 1 John Morgan Building, 3620 Hamilton Walk, Philadelphia, PA 19104 6068, USA
    Toxicol Appl Pharmacol 194:280-95. 2004
    ..We conclude that CO exposure initiates perivascular processes including oxidative stress that triggers activation of NMDA neuronal nNOS, and these events are necessary for the progression of CO-mediated neuropathology...
  18. ncbi request reprint Stimulation of perivascular nitric oxide synthesis by oxygen
    Stephen R Thom
    Department of Emergency Medicine, University of Pennsylvania Medical Center, Philadelphia 19104, USA
    Am J Physiol Heart Circ Physiol 284:H1230-9. 2003
    ..NO associated with hemoglobin. These findings highlight the complexity of oxidative stress responses and may help explain some of the dose responses associated with therapeutic applications of hyperbaric oxygen...
  19. ncbi request reprint Stimulation of nitric oxide synthase in cerebral cortex due to elevated partial pressures of oxygen: an oxidative stress response
    Stephen R Thom
    Department of Emergency Medicine, University of Pennsylvania Medical Center, Philadelphia, PA 19104, USA
    J Neurobiol 51:85-100. 2002
    ..We conclude that hyperoxia causes an increase in (*)NO synthesis as part of a response to oxidative stress. Mechanisms for nNOS activation include augmentation in the association with Hsp90 and intracellular entry of calcium...
  20. ncbi request reprint Stem cell mobilization by hyperbaric oxygen
    Stephen R Thom
    Institute for Environmental Medicine, University of Pennsylvania, Philadelphia, PA 19104 6068, USA
    Am J Physiol Heart Circ Physiol 290:H1378-86. 2006
    ..We conclude that HBO(2) mobilizes stem/progenitor cells by stimulating *NO synthesis...
  21. ncbi request reprint Roles for platelet-activating factor and *NO-derived oxidants causing neutrophil adherence after CO poisoning
    S R Thom
    Department of Emergency Medicine, University of Pennsylvania Medical Center, Philadelphia, Pennsylvania 19104 6068, USA
    Am J Physiol Heart Circ Physiol 281:H923-30. 2001
    ..Persistent PAF-mediated neutrophil adherence required production of *NO-derived oxidants because when oxidants were scavenged, neutrophil adherence was not maintained...
  22. pmc Adaptive responses and apoptosis in endothelial cells exposed to carbon monoxide
    S R Thom
    Institute for Environmental Medicine, University of Pennsylvania Medical Center, Philadelphia, PA 19104 6068, USA
    Proc Natl Acad Sci U S A 97:1305-10. 2000
    ....
  23. ncbi request reprint Nitric oxide synthesis in brain is stimulated by oxygen
    Stephen R Thom
    Department of Emergency Medicine, and the Institute for Environmental Medicine, University of Pennsylvania, Philadelphia, PA 19104 6068, USA
    Adv Exp Med Biol 510:133-7. 2003
  24. pmc Hyperbaric oxygen stimulates vasculogenic stem cell growth and differentiation in vivo
    Tatyana N Milovanova
    Institute for Environmental Medicine, University of Pennsylvania, Philadelphia, PA 19104 6068, USA
    J Appl Physiol (1985) 106:711-28. 2009
    ..Thioredoxin system activation leads to elevations in HIF-1 and -2, followed by synthesis of HIF-dependent growth factors. HIF-3 has a negative impact on SPCs...
  25. pmc Lactate stimulates vasculogenic stem cells via the thioredoxin system and engages an autocrine activation loop involving hypoxia-inducible factor 1
    Tatyana N Milovanova
    Institute for Environmental Medicine, University of Pennsylvania Medical Center, Philadelphia, PA 19104 6068, USA
    Mol Cell Biol 28:6248-61. 2008
    ..Oxidative stress from lactate metabolism by SPCs accelerated further SPC recruitment and differentiation through Trx1-mediated elevations in HIF-1 levels and the subsequent synthesis of HIF-1-dependent growth factors...
  26. pmc Xanthine oxidoreductase and neurological sequelae of carbon monoxide poisoning
    Shih Tsung Han
    Institute for Environmental Medicine, University of Pennsylvania Medical Center, Philadelphia, PA, USA
    Toxicol Lett 170:111-5. 2007
    ..These results indicate that XOR is essential to the pathological cascade of CO-mediated NS...
  27. pmc Diabetic impairments in NO-mediated endothelial progenitor cell mobilization and homing are reversed by hyperoxia and SDF-1 alpha
    Katherine A Gallagher
    Department of Surgery, University of Pennsylvania Medical Center, 3400 Spruce Street, Philadelphia, PA 19104, USA
    J Clin Invest 117:1249-59. 2007
    ..The targets identified, which we believe to be novel, can significantly advance the field of diabetic wound healing...
  28. pmc The differential effect of angiotensin-converting enzyme inhibitors and angiotensin receptor blockers with respect to foot ulcer and limb amputation in those with diabetes
    David J Margolis
    Department of Dermatology and Biostatistics and Epidemiology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
    Wound Repair Regen 18:445-51. 2010
    ..45 (0.22, 0.91) for the new onset of a LEA. In conclusion, among those with diabetes, exposure to ACEi as compared with ARB increases the risk of developing a DFU or LEA...
  29. ncbi request reprint Carbon monoxide-induced deficits in cognitive performance of mice and lack of effect of hyperbaric oxygen treatment
    Stephen R Thom
    Department of Emergency Medicine and Institute for Environmental Medicine, University of Pennsylvania Medical Center, Philadelphia, PA, USA
    Acad Emerg Med 9:75-7. 2002
  30. ncbi request reprint Hyperbaric oxygen and bone marrow-derived endothelial progenitor cells in diabetic wound healing
    Katherine A Gallagher
    Department of Surgery, Institute for Environmental Medicine, University of Pennsylvania Medical Center, Philadelphia 19124, USA
    Vascular 14:328-37. 2006
    ....
  31. ncbi request reprint Endothelial progenitor cell release into circulation is triggered by hyperoxia-induced increases in bone marrow nitric oxide
    Lee J Goldstein
    Department of Surgery, University of Pennsylvania Medical Center, 4th Floor Silverstein Pavilion, 3400 Spruce Street, Philadelphia, Pennsylvania 19124, USA
    Stem Cells 24:2309-18. 2006
    ..These data show that EPC mobilization into circulation is triggered by hyperoxia through induction of bone marrow NO with resulting enhancement in ischemic limb perfusion and wound healing...
  32. doi request reprint Actin S-nitrosylation inhibits neutrophil beta2 integrin function
    Stephen R Thom
    Institute for Environmental Medicine, Department of Emergency Medicine, University of Pennsylvania Medical Center, Philadelphia, Pennsylvania 19104 6068, USA
    J Biol Chem 283:10822-34. 2008
    ..We conclude that cytoskeletal changes triggered by hyperoxia inhibit beta(2) integrin-dependent neutrophil adhesion...
  33. ncbi request reprint Vascular leukocyte sequestration in decompression sickness and prophylactic hyperbaric oxygen therapy in rats
    James D Martin
    Department of Emergency Medicine and Institute for Environmental Medicine, University of Pennsylvania Medical Center, Philadelphia 19104 6068, USA
    Aviat Space Environ Med 73:565-9. 2002
    ....
  34. pmc Myocardial cytochrome oxidase activity is decreased following carbon monoxide exposure
    Kelechi N Iheagwara
    Department of Anesthesiology and Critical Care Medicine, The Children s Hospital of Philadelphia, PA, USA
    Biochim Biophys Acta 1772:1112-6. 2007
    ..Decreased CcOX activity following CO inhalation was likely due to decreased heme aa(3) and CcOX subunit I content. Importantly, myocardial CcOX impairment could underlie CO induced cardiac dysfunction...
  35. ncbi request reprint Effects of hyperbaric oxygen exposure on experimental head and neck tumor growth, oxygenation, and vasculature
    Yuquan Shi
    Department of Radiation Oncology, University of Pennsylvania, 195 John Morgan Building, 37th and Hamilton Walk, Philadelphia, PA 19104 6072, USA
    Head Neck 27:362-9. 2005
    ..Hyperbaric oxygen (HBO2) is used to promote healing in irradiated tissues, but concern persists about the possibility that it may promote residual tumor growth...
  36. ncbi request reprint Vascular reactivity and endothelial NOS activity in rat thoracic aorta during and after hyperbaric oxygen exposure
    Jonas Hink
    Hyperbaric Oxygen Treatment Unit 4092 Dept of Anaesthesia, The Centre of Head and Orthopaedics, Copenhagen Univ Hospital Rigshospitalet, 9 Blegdamsvej, DK 2100 Copenhagen OE, Denmark
    Am J Physiol Heart Circ Physiol 291:H1988-98. 2006
    ..In addition, HBO increases the resting tone of rat aortic rings and attenuates the contractile response to norepinephrine by endothelium-independent mechanisms that involve extraendothelial NO production...
  37. ncbi request reprint Hyperbaric-oxygen therapy for acute carbon monoxide poisoning
    Stephen R Thom
    N Engl J Med 347:1105-6. 2002
  38. ncbi request reprint "Therapeutic" carbon monoxide may be toxic
    Stephen R Thom
    Am J Respir Crit Care Med 171:1318. 2005
  39. ncbi request reprint Hyperbaric oxygen therapy for carbon monoxide poisoning : is it time to end the debates?
    Stephen R Thom
    Toxicol Rev 24:157-8; discussion 159-60. 2005

Research Grants10

  1. CO POISONING IN THE CONTEXT OF A REPERFUSION INJURY
    Stephen Thom; Fiscal Year: 2003
    ..The research plan is aimed to test the hypothesis that immune responses and the associated oxidative stress cause neurological injuries. ..
  2. CO POISONING IN THE CONTEXT OF A REPERFUSION INJURY
    Stephen Thom; Fiscal Year: 1999
    ..Impaired cGMP synthesis will be investigated as a possible mechanism for inhibition of B2 integrin function caused by both nitric oxide and hyperbaric oxygen treatment of CO-poisoned rats. ..