Research Topics
| DOUGLAS DILLON TAYLORSummaryAffiliation: University of Louisville Country: USA Publications
Research Grants
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Detail Information
Publications
Shed membrane fragment-associated markers for endometrial and ovarian cancersDouglas D Taylor
Department of Obstetrics, Gynecology, and Women s Health, University of Louisville School of Medicine, Louisville, Kentucky 40292, USA
Gynecol Oncol 84:443-8. 2002..The objective of this study was to assess circulating tumor-derived membrane fragments (MFs) and specific proteins associated with them as diagnostic and prognostic markers for ovarian and endometrial cancers...- Modulation of T-cell CD3-zeta chain expression during normal pregnancyDouglas D Taylor
Department of Obstetrics and Gynecology, University of Louisville School of Medicine, Louisville, KY 40292, USA
J Reprod Immunol 54:15-31. 2002..Suppression of zeta expression appears to be due to a circulating low-molecular-weight protein that suppresses CD3-zeta in a concentration-dependent manner... 
Tumour-derived exosomes and their role in cancer-associated T-cell signalling defectsD D Taylor
Department of Obstetrics, Gynecology and Women s Health, University of Louisville School of Medicine, Louisville, KY 40202, USA
Br J Cancer 92:305-11. 2005..These results indicate that, while 'exosomes' express tumour antigens, leading to their proposed utility as tumour vaccines, they also can suppress T-cell signalling molecules and induce apoptosis...- Pregnancy-linked suppression of TcR signaling pathways by a circulating factor absent in recurrent spontaneous pregnancy loss (RPL)Douglas D Taylor
Department of Obstetrics, Gynecology, and Women s Health, Division of Gynecologic Oncology, University of Louisville School of Medicine, 511 South Floyd Street, MDR 420, Louisville, KY 40292, USA
Mol Immunol 43:1872-80. 2006..The 14 kDa circulating ZIP from normal pregnancies suppressed components within the JAK/STAT pathway and induced suppressors of cytokine stimulation, SOCS2... - Pregnancy-associated exosomes and their modulation of T cell signalingDouglas D Taylor
Department of Obstetrics, Gynecology, and Women s Health, University of Louisville School of Medicine, Louisville, KY 40202, USA
J Immunol 176:1534-42. 2006.... 
MicroRNA signatures of tumor-derived exosomes as diagnostic biomarkers of ovarian cancerDouglas D Taylor
Department of Obstetrics, Gynecology, and Women s Health, University of Louisville School of Medicine, Louisville, KY 40202, USA
Gynecol Oncol 110:13-21. 2008..Tumors actively release exosomes into the peripheral circulation and we now demonstrate the association of microRNAs with circulating tumor-derived exosomes...- Alterations of T cell activation signalling and cytokine production by postmenopausal estrogen levelsLowell T Ku
Division of Reproductive Endocrinology and Infertility, Department of Obstetrics, Gynecology and Women s Health, University of Louisville School of Medicine, Louisville, Kentucky, USA
Immun Ageing 6:1. 2009..Lower signalling protein levels appear to result from decreased CD3-zeta, JAK2, and JAK3 gene expressions. These findings may provide a molecular basis for immunosenescence associated with the postmenopausal state... - Patient-derived tumor-reactive antibodies as diagnostic markers for ovarian cancerDouglas D Taylor
Division of Gynecologic Oncology, Department of Obstetrics, Gynecology and Women s Health, University of Louisville School of Medicine, Louisville, KY 40292, USA
Gynecol Oncol 115:112-20. 2009..Our objective was to validate our array assay for the identification of ovarian cancer based on quantitation of tumor-reactive IgG... - Characterization of humoral responses of ovarian cancer patients: antibody subclasses and antigenic componentsDouglas D Taylor
Division of Gynecologic Oncology, Department of Obstetrics, Gynecology and Women s Health, University of Louisville School of Medicine, Louisville, KY 40292, USA
Gynecol Oncol 116:213-21. 2010..In contrast, tumor antigen immunorecognition is observed following initial transformation events. Our objective was to characterize humoral antitumor responses in terms of IgG subclasses generated and tumor antigens recognized... - Exosome isolation for proteomic analyses and RNA profilingDouglas D Taylor
James Graham Brown Cancer Center, University of Louisville School of Medicine, Louisville, KY, USA
Methods Mol Biol 728:235-46. 2011.... - Alterations in T-cell signal transduction molecules associated with recurrent spontaneous pregnancy lossDouglas D Taylor
Department of Obstetrics and Gynecology, University of Louisville, School of Medicine, 511 S Floyd Street, MDR 420, Louisville, KY 40202, USA
J Reprod Immunol 63:137-54. 2004..Our findings indicate that women experiencing RSA fail to suppress CD3-zeta and JAK3, suggesting a deficiency in this circulating factor that induces their suppression in normal pregnancy... - Modulation of CD3-zeta as a marker of clinical response to IL-2 therapy in ovarian cancer patientsDouglas D Taylor
Division of Gynecologic Oncology, University of Louisville School of Medicine, Louisville, KY 40292, USA
Gynecol Oncol 94:54-60. 2004..The aims of this study were to identify the circulating component that modulated zeta expression and to define whether this suppressive activity could serve as a marker of biotherapy responsiveness... - Exosomes/microvesicles: mediators of cancer-associated immunosuppressive microenvironmentsDouglas D Taylor
Department of Obstetrics, Gynecology and Women s Health and James Graham Brown Cancer Center, University of Louisville School of Medicine, Louisville, KY, USA
Semin Immunopathol 33:441-54. 2011..By understanding the nature of these tumor-derived exosomes/microvesicles and their roles in mediating cancer progression and modulating the host immune response will significantly impact therapeutic approaches targeting exosomes... - T-cell apoptosis and suppression of T-cell receptor/CD3-zeta by Fas ligand-containing membrane vesicles shed from ovarian tumorsDouglas D Taylor
Departments of Obstetrics and Gynecology and Radiation Oncology, University of Louisville School of Medicine, Louisville, Kentucky 40202, USA
Clin Cancer Res 9:5113-9. 2003.... - Genistein reverses diminished T-cell signal transduction, induced by post-menopausal estrogen levelsJ Preston Parry
Division of Reproductive Endocrinology and Infertility, Department of Obstetrics, Gynecology, and Women s Health, University of Louisville School of Medicine, Louisville, KY 40202, USA
Am J Reprod Immunol 61:26-33. 2009..This study addressed the ability of genistein to reverse the loss of T-cell signaling components, induced by estrogen deficiency associated with aging... - Potentiation of the radiation effect with genistein in cervical cancer cellsCatheryn M Yashar
Department of Radiation Oncology, University of Louisville School of Medicine, 511 South Floyd Street, Room 416, Louisville, KY 40202, USA
Gynecol Oncol 99:199-205. 2005..CONCLUSIONS.: Genistein inhibits growth of cervical cancer cells. Genistein results in variable and significant enhancement of the radiation effect that may be partially mediated by G(2)M arrest, Mcl-1 and activation of the AKT gene... - Shed membrane fragment modulation of CD3-zeta during pregnancy: link with induction of apoptosisCicek Gercel-Taylor
Department of Obstetrics, Gynecology, and Women's Health, University of Louisville School of Medicine, 511 South Floyd Street, MDR 420, Louisville, KY 40292, USA
J Reprod Immunol 56:29-44. 2002..These membrane fragment isolates are capable of inducing FasL-mediated apoptosis and down-regulating CD3-zeta expression, which may contribute to the immune tolerance of the fetus... - Loss of communication in ovarian cancerChris R Toler
Division of Gynecologic Oncology, Department of Obstetrics, Gynecology and Women s Health, University of Louisville School of Medicine, Louisville, KY, USA
Am J Obstet Gynecol 194:e27-31. 2006..This study was undertaken to investigate the expression of connexins in ovarian cancer cell lines and assess correlation with chemosensitivity... - Induction of p53 and drug resistance following treatment with cisplatin or paclitaxel in ovarian cancer cell linesDaniel S Metzinger
Department of Obstetrics, Gynecology and Women's Health, Division of Gynecologic Oncology, University of Louisville School of Medicine, 511 South Floyd Street, MDR Building, Room 416, Louisville, KY 40202, USA
Cancer Lett 236:302-8. 2006..9 microg/ml, while UL-3B clones ranged from 11.8-252.0 microg/ml, with a mean value of 73.2 microg/ml for Cis, and 260.0-4400.0 nM (mean 2555.0 nM) for Tax. Selection pressures during treatment may contribute to drug resistance... - Inhibitory effect of genistein and daidzein on ovarian cancer cell growthCicek Gercel-Taylor
Department of Obstetrics, Gynecology and Women s Health, University of Louisville, School of Medicine, Louisville, KY 40202, USA
Anticancer Res 24:795-800. 2004..Survival from ovarian cancer has not changed significantly in the past twenty years requiring development of additional treatment protocols. We studied the effect of genistein and daidzein on ovarian cancer cell growth... - HSP-10 in ovarian cancer: expression and suppression of T-cell signalingSibel Akyol
Division of Gynecologic Oncology, Department of Obstetrics, Gynecology, and Women's Health, University of Louisville School of Medicine, 511 South Floyd Street, MDR 420, Louisville, KY 40292, USA
Gynecol Oncol 101:481-6. 2006..Our findings indicate that, as in pregnancy, production and release of HSP10 may be a critical factor in the suppression of T-cell activation, allowing the tumor to escape immune surveillance... - Endometriosis markers: immunologic alterations as diagnostic indicators for endometriosisHenry C Bohler
Department of Obstetrics, Gynecology and Women s Health, University of Louisville School of Medicine, Louisville, Kentucky, USA
Reprod Sci 14:595-604. 2007..Alterations in autoreactivity and NK activity are observed in endometriosis and may be useful as diagnostic markers, even in early stage disease... - Effect of chemotherapy on the mutation frequency of ovarian cancer cells at the HPRT locusCicek Gercel-Taylor
Department of Obstetrics and Gynecology, University of Louisville School of Medicine, Louisville, KY 40202, USA
Anticancer Res 25:2113-7. 2005..Our objective was to demonstrate the effect of chemotherapeutic agents, used in the treatment of ovarian cancer, on the mutation frequency of ovarian cancer cells at the HPRT locus... - Cellular response to chemotherapy and radiation in cervical cancerAngela Saxena
Department of Obstetrics, Gynecology, and Women's Health, University of Louisville School of Medicine, KY 40202, USA
Am J Obstet Gynecol 192:1399-403. 2005..These data provide correlation of response to combined therapeutic modalities with HPV status of cervical cancer and expression of survivin and AKT... - Gene expression profiling in response to estradiol and genistein in ovarian cancer cellsLynn P Parker
Division of Gynecologic Oncology, Department of Obstetrics, Gynecology and Women s Health, University of Louisville School of Medicine, Louisville, KY 40202, USA
Cancer Genomics Proteomics 6:189-94. 2009..We analyzed the gene-expression in response to estradiol (E2) and genistein (Gen) in ovarian cancer cells... - Exosomal microRNA: a diagnostic marker for lung cancerGuilherme Rabinowits
J G Brown Cancer Center, University of Louisville, KY, USA
Clin Lung Cancer 10:42-6. 2009..No correlation between the exosomal miRNA levels and the stage of disease can be made at this point... - Modulation of T-cell CD3-zeta chain expression in early pregnancyAbby C Eblen
Department of Obstetrics, Gynecology and Women's Health, University of Louisville School of Medicine, KY, USA
Am J Reprod Immunol 47:167-73. 2002..9%, P < 0.0001). CONCLUSION: A factor capable of suppressing TcR/CD3-zeta expression can be detected in the sera of pregnant women 14 days after embryo retrieval. Loss of zeta chain was associated with the induction of apoptosis... - A novel approach to cancer immunotherapy: tumor cells decorated with CD80 generate effective antitumor immunityNarendra P Singh
Institute for Cellular Therapeutics, University of Louisville, Louisville, Kentucky 40202, USA
Cancer Res 63:4067-73. 2003..This technology may serve as a fast, efficient, and safe alternative to gene transfer approaches for engineering tumor cells for use in immunotherapy and research... - Specific isolation of placenta-derived exosomes from the circulation of pregnant women and their immunoregulatory consequencesAnuradha Sabapatha
Department of Obstetrics, Gynecology, and Women s Health, University of Louisville School of Medicine, Louisville, KY 40202, USA
Am J Reprod Immunol 56:345-55. 2006..In normal pregnancy, as factors linked with early immunomodulation decline, placental exosomes become critical in modulating T-cell activation, suppressing effector T cells by enhancing lymphocyte apoptosis and CD3-zeta loss... - Differential expression of TcR-CD3 zeta as evidence for altered immunoregulation in preeclamptic versus normotensive womenGarrett K Lam
Division of Maternal Fetal Medicine, Department of Obstetrics and Gynecology, University of North Carolina School of Medicine, Chapel Hill, USA
Am J Obstet Gynecol 189:843-7. 2003..The study was undertaken to exhibit and quantify the difference in modulation of CD3-zeta protein (an integral component of the T-cell receptor) in preeclamptic and normotensive women... - Primary tumor cells resected from cancer patients and decorated with a novel form of CD80 protein serve as effective antigen-presenting cells for the induction of autologous T cell immune responses ex vivoNarendra P Singh
Institute for Cellular Therapeutics and Department of Microbiology and Immunology, University of Louisville, Louisville, KY 40202, USA
Hum Gene Ther 17:334-46. 2006..Taken together, these data demonstrate that primary tumor cells can be effectively decorated with CD80-SA and that such cells serve as APCs to induce autologous antitumor T cell responses... - Urinary matrix metalloproteinases as a potential screening test for gynecologic malignanciesLisa B Bazzett
Department of Obstetrics and Gynecology, Division of Gynecologic Oncology, Ochnsner Clinic Foundation, New Orleans, LA 70121, USA
Gynecol Oncol 90:435-42. 2003..This was a pilot study to determine the feasibility of using urinary matrix metalloproteinases (MMPs) as a screening test for gynecologic malignancies...
Research Grants
- Tumor-reactive IgG in the diagnosis of ovarian cancerDouglas Taylor; Fiscal Year: 2005..Ultimately, antigenic proteins, linked with histologic type and stage of disease, will be combined into the development of protein arrays to screen for circulating reactive IgG as an early diagnostic test. ..