Robert Tarran

Summary

Affiliation: University of North Carolina
Country: USA

Publications

  1. ncbi request reprint Mammalian short palate lung and nasal epithelial clone 1 (SPLUNC1) in pH-dependent airway hydration
    Robert Tarran
    Cystic Fibrosis Pulmonary Research and Treatment Center, Department of Cell Biology and Physiology, University of North Carolina, Chapel Hill, NC 27599, USA Electronic address
    Int J Biochem Cell Biol 52:130-5. 2014
  2. pmc Identification of the SPLUNC1 ENaC-inhibitory domain yields novel strategies to treat sodium hyperabsorption in cystic fibrosis airway epithelial cultures
    Carey A Hobbs
    Cystic Fibrosis Pulmonary Research and Treatment Center, 7125 Thurston Bowles Bldg, UNC, Chapel Hill, NC 27599 7248
    Am J Physiol Lung Cell Mol Physiol 305:L990-L1001. 2013
  3. pmc Does epithelial sodium channel hyperactivity contribute to cystic fibrosis lung disease?
    Carey A Hobbs
    R Tarran 7125 Thurston Bowles Building, UNC, Chapel Hill, NC 27599 7248, USA
    J Physiol 591:4377-87. 2013
  4. pmc Nonantibiotic macrolides prevent human neutrophil elastase-induced mucus stasis and airway surface liquid volume depletion
    Robert Tarran
    Cystic Fibrosis Pulmonary Research and Treatment Center, University of North Carolina, Chapel Hill, NC 27516, USA
    Am J Physiol Lung Cell Mol Physiol 304:L746-56. 2013
  5. pmc Soluble mediators, not cilia, determine airway surface liquid volume in normal and cystic fibrosis superficial airway epithelia
    Robert Tarran
    Cystic Fibrosis Pulmonary Research and Treatment Center, The University of North Carolina, Chapel Hill 27599, USA
    J Gen Physiol 127:591-604. 2006
  6. ncbi request reprint Regulation of normal and cystic fibrosis airway surface liquid volume by phasic shear stress
    Robert Tarran
    Cystic Fibrosis Pulmonary Research and Treatment Center, University of North Carolina, Chapel Hill, North Carolina 27599 7248, USA
    Annu Rev Physiol 68:543-61. 2006
  7. pmc Identification of SPLUNC1's ENaC-inhibitory domain yields novel strategies to treat sodium hyperabsorption in cystic fibrosis airways
    Carey A Hobbs
    Cystic Fibrosis Pulmonary Research and Treatment Center, Department of Prosthodontics, University of North Carolina, Chapel Hill, 7125 Thurston Bowles Bldg, Chapel Hill, NC 27599 7248, USA
    FASEB J 26:4348-59. 2012
  8. pmc Normal and cystic fibrosis airway surface liquid homeostasis. The effects of phasic shear stress and viral infections
    Robert Tarran
    Cystic Fibrosis Pulmonary Research and Treatment Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 7248, USA
    J Biol Chem 280:35751-9. 2005
  9. pmc Molecular basis for pH-dependent mucosal dehydration in cystic fibrosis airways
    Alaina L Garland
    Cystic Fibrosis Pulmonary Research and Treatment Center, Department of Chemistry, Department of Biochemistry and Biophysics, Departments of Prosthodontics and Pharmacology, Department of Microbiology and Immunology, and Department of Cell Biology and Physiology, University of North Carolina, Chapel Hill, NC 27599
    Proc Natl Acad Sci U S A 110:15973-8. 2013
  10. pmc SPLUNC1 regulates airway surface liquid volume by protecting ENaC from proteolytic cleavage
    Agustin Garcia-Caballero
    Cystic Fibrosis Pulmonary Research and Treatment Center, Thurston Bowles Building, University of North Carolina, Chapel Hill, NC 27599 7248, USA
    Proc Natl Acad Sci U S A 106:11412-7. 2009

Research Grants

  1. Ion Transport and Mucus Clearance in CF Airways
    Robert Tarran; Fiscal Year: 2005
  2. Ion Transport and Mucus Clearance in CF Airways
    Robert Tarran; Fiscal Year: 2006
  3. Ion Transport and Mucus Clearance in CF Airways
    Robert Tarran; Fiscal Year: 2007

Collaborators

Detail Information

Publications32

  1. ncbi request reprint Mammalian short palate lung and nasal epithelial clone 1 (SPLUNC1) in pH-dependent airway hydration
    Robert Tarran
    Cystic Fibrosis Pulmonary Research and Treatment Center, Department of Cell Biology and Physiology, University of North Carolina, Chapel Hill, NC 27599, USA Electronic address
    Int J Biochem Cell Biol 52:130-5. 2014
    ..This review will focus on the latter function of SPLUNC1, and will discuss new structural and physiological data regarding SPLUNC1's failure to function as a regulator of ASL hydration in CF airways...
  2. pmc Identification of the SPLUNC1 ENaC-inhibitory domain yields novel strategies to treat sodium hyperabsorption in cystic fibrosis airway epithelial cultures
    Carey A Hobbs
    Cystic Fibrosis Pulmonary Research and Treatment Center, 7125 Thurston Bowles Bldg, UNC, Chapel Hill, NC 27599 7248
    Am J Physiol Lung Cell Mol Physiol 305:L990-L1001. 2013
    ..Furthermore, the robust inhibition of ENaC by the S18 peptide suggests that this peptide may be suitable for treating CF lung disease. ..
  3. pmc Does epithelial sodium channel hyperactivity contribute to cystic fibrosis lung disease?
    Carey A Hobbs
    R Tarran 7125 Thurston Bowles Building, UNC, Chapel Hill, NC 27599 7248, USA
    J Physiol 591:4377-87. 2013
    ..This review will focus on our current understanding of the contribution of ENaC to CF pathogenesis...
  4. pmc Nonantibiotic macrolides prevent human neutrophil elastase-induced mucus stasis and airway surface liquid volume depletion
    Robert Tarran
    Cystic Fibrosis Pulmonary Research and Treatment Center, University of North Carolina, Chapel Hill, NC 27516, USA
    Am J Physiol Lung Cell Mol Physiol 304:L746-56. 2013
    ..We conclude that GS-459755 may limit HNE-induced activation of ENaC and may be useful for the treatment of mucus dehydration in CF and COPD without inducing bacterial resistance...
  5. pmc Soluble mediators, not cilia, determine airway surface liquid volume in normal and cystic fibrosis superficial airway epithelia
    Robert Tarran
    Cystic Fibrosis Pulmonary Research and Treatment Center, The University of North Carolina, Chapel Hill 27599, USA
    J Gen Physiol 127:591-604. 2006
    ..Interestingly, the amount of ASL/Cl- secretion elicited by ATP/ADO was influenced by the level of CAP-induced Na+ absorption, suggesting that there are important interactions between the soluble regulators which finely tune ASL volume...
  6. ncbi request reprint Regulation of normal and cystic fibrosis airway surface liquid volume by phasic shear stress
    Robert Tarran
    Cystic Fibrosis Pulmonary Research and Treatment Center, University of North Carolina, Chapel Hill, North Carolina 27599 7248, USA
    Annu Rev Physiol 68:543-61. 2006
    ..We also discuss (a) how derangement of the ADO-CFTR pathway renders CF airways vulnerable to viral infections that deplete ASL volume and produce mucus stasis, and (b) potential shear stress-dependent therapies for CF...
  7. pmc Identification of SPLUNC1's ENaC-inhibitory domain yields novel strategies to treat sodium hyperabsorption in cystic fibrosis airways
    Carey A Hobbs
    Cystic Fibrosis Pulmonary Research and Treatment Center, Department of Prosthodontics, University of North Carolina, Chapel Hill, 7125 Thurston Bowles Bldg, Chapel Hill, NC 27599 7248, USA
    FASEB J 26:4348-59. 2012
    ..Furthermore, the robust inhibition of ENaC by the G22-A39 peptide suggests that this peptide may be suitable for treating CF lung disease...
  8. pmc Normal and cystic fibrosis airway surface liquid homeostasis. The effects of phasic shear stress and viral infections
    Robert Tarran
    Cystic Fibrosis Pulmonary Research and Treatment Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 7248, USA
    J Biol Chem 280:35751-9. 2005
    ....
  9. pmc Molecular basis for pH-dependent mucosal dehydration in cystic fibrosis airways
    Alaina L Garland
    Cystic Fibrosis Pulmonary Research and Treatment Center, Department of Chemistry, Department of Biochemistry and Biophysics, Departments of Prosthodontics and Pharmacology, Department of Microbiology and Immunology, and Department of Cell Biology and Physiology, University of North Carolina, Chapel Hill, NC 27599
    Proc Natl Acad Sci U S A 110:15973-8. 2013
    ..Together, these data provide molecular insights into the mucosal dehydration associated with a range of pulmonary diseases, including CF, and suggest that future therapy be directed toward alkalinizing the pH of CF airways. ..
  10. pmc SPLUNC1 regulates airway surface liquid volume by protecting ENaC from proteolytic cleavage
    Agustin Garcia-Caballero
    Cystic Fibrosis Pulmonary Research and Treatment Center, Thurston Bowles Building, University of North Carolina, Chapel Hill, NC 27599 7248, USA
    Proc Natl Acad Sci U S A 106:11412-7. 2009
    ..Thus, we propose that SPLUNC1 is secreted onto mucosal surfaces as a soluble volume sensor whose concentration and dilution can regulate ENaC activity and mucosal volumes, including that of airway surface liquid...
  11. pmc 17beta-Estradiol inhibits Ca2+-dependent homeostasis of airway surface liquid volume in human cystic fibrosis airway epithelia
    Ray D Coakley
    Cystic Fibrosis Pulmonary Research and Treatment Center, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    J Clin Invest 118:4025-35. 2008
    ....
  12. pmc Cigarette smoke exposure induces CFTR internalization and insolubility, leading to airway surface liquid dehydration
    Lucy A Clunes
    Cystic Fibrosis Pulmonary Research and Treatment Center, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    FASEB J 26:533-45. 2012
    ..Furthermore, our data suggest that strategies to rehydrate airway surfaces may provide a novel form of therapy for patients with CB...
  13. pmc SPLUNC1 expression reduces surface levels of the epithelial sodium channel (ENaC) in Xenopus laevis oocytes
    Brett M Rollins
    Cystic Fibrosis Pulmonary Research and Treatment Center, The University of North Carolina, Chapel Hill, NC, USA
    Channels (Austin) 4:255-9. 2010
    ..A better understanding of ENaC's regulation by endogenous inhibitors may aid in the development of novel therapies designed to inhibit hyperactive ENaC in cystic fibrosis epithelia...
  14. pmc A2B adenosine receptors regulate the mucus clearance component of the lung's innate defense system
    Brett M Rollins
    Cystic Fibrosis Pulmonary Research and Treatment Center, The University of North Carolina, Chapel Hill, North Carolina, USA
    Am J Respir Cell Mol Biol 39:190-7. 2008
    ..A(2B)-R are required for ASL volume homeostasis in human airways, and therapies directed at inhibiting A(2B)-R may lead to a cystic fibrosis-like phenotype with depleted ASL volume and mucus stasis...
  15. pmc Defective adenosine-stimulated cAMP production in cystic fibrosis airway epithelia: a novel role for CFTR in cell signaling
    Michael J Watson
    Cystic Fibrosis Pulmonary Research and Treatment Center, The University of North Carolina, Chapel Hill, NC 27599 7248, USA
    FASEB J 25:2996-3003. 2011
    ..Thus, we hypothesize that CFTR regulates A2BR levels in the plasma membrane to modulate cell signaling and to enhance selective components of the innate lung defense system...
  16. pmc Nucleotide release provides a mechanism for airway surface liquid homeostasis
    Eduardo R Lazarowski
    Cystic Fibrosis Pulmonary Research and Treatment Center, University of North Carolina School of Medicine, 7017 Thurston Bowles Building, Chapel Hill, NC 72599, USA
    J Biol Chem 279:36855-64. 2004
    ..Our data reveal a complex pattern of nucleotides/nucleosides in ASL under resting conditions and suggest that adenosine may play a key role in regulating ASL volume homeostasis...
  17. pmc Liquid movement across the surface epithelium of large airways
    Lucy A Chambers
    Cystic Fibrosis Pulmonary Research and Treatment Center, University of North Carolina, Chapel Hill, NC 27599 7248, USA
    Respir Physiol Neurobiol 159:256-70. 2007
    ..CFTR as a cAMP-dependent regulator of sodium channels. Science 269, 847-850]. In this chapter, we will discuss the regulation of these two ion channels, and how they can influence liquid movement across the superficial airway epithelium...
  18. ncbi request reprint The cystic fibrosis transmembrane conductance regulator is regulated by a direct interaction with the protein phosphatase 2A
    William R Thelin
    Department of Cell and Developmental Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    J Biol Chem 280:41512-20. 2005
    ..Furthermore, PP2A may be a clinically relevant drug target for CF, which should be considered in future studies...
  19. pmc A mechanochemical model for auto-regulation of lung airway surface layer volume
    Gregory Herschlag
    Department of Mathematics, University of North Carolina, Chapel Hill, NC, USA
    J Theor Biol 325:42-51. 2013
    ....
  20. pmc Characterization of the oligomeric structure of the Ca(2+)-activated Cl- channel Ano1/TMEM16A
    John T Sheridan
    Department of Cell and Molecular Physiology, University of North Carolina, North Carolina, Chapel Hill 27599, USA
    J Biol Chem 286:1381-8. 2011
    ..These data are the first to probe the quaternary structure of Ano1. Understanding the oligomeric nature of Ano1 is an essential step in the development of therapeutic drugs that could be useful in the treatment of cystic fibrosis...
  21. ncbi request reprint Rationale for hypertonic saline therapy for cystic fibrosis lung disease
    Robert Tarran
    Cystic Fibrosis Pulmonary Research and Treatment Center, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 7248, USA
    Semin Respir Crit Care Med 28:295-302. 2007
    ....
  22. doi request reprint Methods for ASL measurements and mucus transport rates in cell cultures
    Erin N Worthington
    Cystic Fibrosis Pulmonary Research and Treatment Center, The University of North Carolina, Chapel Hill, NC 27599 7248, USA
    Methods Mol Biol 742:77-92. 2011
    ....
  23. ncbi request reprint Mucus clearance and lung function in cystic fibrosis with hypertonic saline
    Scott H Donaldson
    University of North Carolina at Chapel Hill Cystic Fibrosis Research and Treatment Center, Chapel Hill 27599, USA
    N Engl J Med 354:241-50. 2006
    ..Through osmotic forces, hypertonic saline may increase the volume of airway surface liquid, restore mucus clearance, and improve lung function...
  24. pmc Regulation of murine airway surface liquid volume by CFTR and Ca2+-activated Cl- conductances
    Robert Tarran
    Cystic Fibsosis Pulmonary Research and Treatment Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    J Gen Physiol 120:407-18. 2002
    ..g., mucosal nucleotides). We conclude that both CaCC and the Ca(2+) signal are increased in CF airway epithelia, and that they contribute to acute but not basal regulation of ASL height...
  25. ncbi request reprint Regulation of airway surface liquid volume and mucus transport by active ion transport
    Robert Tarran
    Thurston Bowles Building, The University of North Carolina, Chapel Hill, NC 27599 7248, USA
    Proc Am Thorac Soc 1:42-6. 2004
    ....
  26. ncbi request reprint Thin-film measurements of airway surface liquid volume/composition and mucus transport rates in vitro
    Robert Tarran
    Cystic Fibrosis Pulmonary Research and Treatment Center, University of North Carolina, Chapel Hill, NC, USA
    Methods Mol Med 70:479-92. 2002
  27. pmc Regulator of G-protein signaling-21 (RGS21) is an inhibitor of bitter gustatory signaling found in lingual and airway epithelia
    Staci P Cohen
    Department of Pharmacology, University of North Carolina Neuroscience Center, University of North Carolina, Chapel Hill, North Carolina 27599 7365, USA
    J Biol Chem 287:41706-19. 2012
    ..Our data collectively suggests that RGS21 modulates bitter taste signal transduction...
  28. pmc In vivo versus in vitro airway surface liquid nicotine levels following cigarette smoke exposure
    Lucy A Clunes
    University of North Carolina at Chapel Hill, CF Center, Chapel Hill, North Carolina, USA
    J Anal Toxicol 32:201-7. 2008
    ..These results suggest that nicotine is a good dosimetry marker of WCS exposure and provides direct evidence that the use of WCS is more relevant than the use of CSC for in vitro systems...
  29. pmc In vivo airway surface liquid Cl- analysis with solid-state electrodes
    Ray A Caldwell
    Cystic Fibrosis Pulmonary Research and Treatment Center, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    J Gen Physiol 119:3-14. 2002
    ..3 +/- 7.1 mM, n = 5) and monkey (128.5 +/- 6.8 mM, n = 3). The measured [Cl-], in excess of 100 mM throughout all airway regions tested in multiple species, is consistent with the isotonic volume hypothesis to describe ASL physiology...
  30. ncbi request reprint Measurements of airway surface liquid height and mucus transport by fluorescence microscopy, and of ion composition by X-ray microanalysis
    Godfried M Roomans
    Department of Medical Cell Biology, University of Uppsala, Box 571, SE 75123 Uppsala, Sweden
    J Cyst Fibros 3:135-9. 2004
    ..The paper describes methods to determine the height of the ASL and corresponding mucus transport rates using fluorescent probes, and methods to determine the ionic composition of the ASL by X-ray microanalysis...
  31. ncbi request reprint Murine epithelial cells: isolation and culture
    Donald J Davidson
    B C Research Institute, Room 381, University of British Columbia, 950 West 28th Avenue, Vancouver, BC, Canada V5Z 4H4
    J Cyst Fibros 3:59-62. 2004
    ....
  32. pmc Effects of reduced mucus oxygen concentration in airway Pseudomonas infections of cystic fibrosis patients
    Dieter Worlitzsch
    Institute of General and Environmental Hygiene, University of Tubingen, Tubingen, Germany
    J Clin Invest 109:317-25. 2002
    ..These studies indicate that novel therapies for CF include removal of hypoxic mucus plaques and antibiotics effective against P. aeruginosa adapted to anaerobic environments...

Research Grants3

  1. Ion Transport and Mucus Clearance in CF Airways
    Robert Tarran; Fiscal Year: 2005
    ..Based on these observations, the specific aims of this proposal are (1) to identify sensors of ASL volume, (2) to understand how ASL volume regulation is transduced and (3) to normalize CF airway surface liquid volume regulation. ..
  2. Ion Transport and Mucus Clearance in CF Airways
    Robert Tarran; Fiscal Year: 2006
    ..Based on these observations, the specific aims of this proposal are (1) to identify sensors of ASL volume, (2) to understand how ASL volume regulation is transduced and (3) to normalize CF airway surface liquid volume regulation. ..
  3. Ion Transport and Mucus Clearance in CF Airways
    Robert Tarran; Fiscal Year: 2007
    ..Based on these observations, the specific aims of this proposal are (1) to identify sensors of ASL volume, (2) to understand how ASL volume regulation is transduced and (3) to normalize CF airway surface liquid volume regulation. ..