Research Topics
| Adel M TalaatSummaryAffiliation: University of Wisconsin Country: USA Publications
Research Grants
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Detail Information
Publications
Genome sequencing of ovine isolates of Mycobacterium avium subspecies paratuberculosis offers insights into host associationJohn P Bannantine
National Animal Disease Center, USDA Agricultural Research Service, Ames, Iowa, USA
BMC Genomics 13:89. 2012..To systematically characterize all genomic differences that may be associated with the specific hosts, we sequenced the genomes of three U.S. sheep isolates and also obtained an optical map...- Mycobacterial bacilli are metabolically active during chronic tuberculosis in murine lungs: insights from genome-wide transcriptional profilingAdel M Talaat
Laboratory of Bacterial Genomics, Department of Pathobiological Sciences, University of Wisconsin Madison, Madison, WI 53706 1581, USA
J Bacteriol 189:4265-74. 2007..A similar approach can be adopted to study key stages of intracellular pathogens... - Whole-genome plasticity among Mycobacterium avium subspecies: insights from comparative genomic hybridizationsChia Wei Wu
Department of Animal Health and Biomedical Sciences, University of Wisconsin Madison, 1656 Linden Drive, Madison, WI 53706 1581
J Bacteriol 188:711-23. 2006..The presented findings will affect the future design of diagnostics and vaccines for Johne's and Crohn's diseases and provide a model for genomic analysis of closely related bacteria... - Expression library immunization: a road map for discovery of vaccines against infectious diseasesAdel M Talaat
Department of Animal Health and Biomedical Sciences, University of Wisconsin-Madison, 1656 Linden Drive, Madison, WI 53706-1581, USA
Infect Immun 73:7089-98. 2005 - mosR, a novel transcriptional regulator of hypoxia and virulence in Mycobacterium tuberculosisBassam Abomoelak
Department of Pathobiological Sciences, University of Wisconsin Madison, Madison, WI 53706, USA
J Bacteriol 191:5941-52. 2009.... - CtpV: a putative copper exporter required for full virulence of Mycobacterium tuberculosisSarah K Ward
Department of Pathobiological Sciences, University of Wisconsin Madison, 1656 Linden Drive, Madison, WI 53706, USA
Mol Microbiol 77:1096-110. 2010..tuberculosis, supporting the hypothesis that copper response could be important to intracellular pathogens, in general... - A novel cell wall lipopeptide is important for biofilm formation and pathogenicity of Mycobacterium avium subspecies paratuberculosisChia Wei Wu
The Laboratory of Bacterial Genomics, Department of Pathobiological Sciences, University of Wisconsin Madison, 1656 Linden Drive, Madison, WI 53706 1581, USA
Microb Pathog 46:222-30. 2009..ap. Molecular tools to better control M. ap infections could be developed utilizing the presented findings... - Defining the stressome of Mycobacterium avium subsp. paratuberculosis in vitro and in naturally infected cowsChia Wei Wu
Laboratory of Bacterial Genomics, Department of Pathobiological Sciences, University of Wisconsin Madison, 1656 Linden Drive, Madison, WI 53706 1581, USA
J Bacteriol 189:7877-86. 2007..Overall, the employed approach profiled mycobacterial genetic networks triggered by variable stressors and identified a novel set of putative virulence genes. A similar approach could be applied to analyze other intracellular pathogens... - Characterization of a novel heat shock protein (Hsp22.5) involved in the pathogenesis of Mycobacterium tuberculosisBassam Abomoelak
Department of Pathobiological Sciences, University of Wisconsin Madison, 1656 Linden Drive, Madison, Wisconsin 53706 1581, USA
J Bacteriol 193:3497-505. 2011..Overall, we suggest that rv0990c encodes a heat shock protein that plays an important role in mycobacterial virulence. Hence, we renamed rv0990c heat shock protein 22.5 (hsp22.5), reflecting its molecular mass... - Invasion and persistence of Mycobacterium avium subsp. paratuberculosis during early stages of Johne's disease in calvesChia Wei Wu
The Laboratory of Bacterial Genomics, Department of Pathobiological Sciences, University of Wisconsin Madison, 1656 Linden Drive, Madison, WI 53706 1581, USA
Infect Immun 75:2110-9. 2007..Overall, novel insights into the early stages of Johne's disease were obtained, and a practical model of mycobacterial invasiveness was developed. A similar approach can be used for other enteric bacteria... - Optical mapping of the Mycobacterium avium subspecies paratuberculosis genomeChia Wei Wu
The Laboratory of Bacterial Genomics, Department of Pathobiological Sciences, University of Wisconsin Madison, WI, USA
BMC Genomics 10:25. 2009..In an effort to better examine diversity among M. ap strains, we used optical mapping to profile genomic variations between strains of M. ap K-10 (sequenced strain) and M. ap ATCC 19698 (type strain)... - The global responses of Mycobacterium tuberculosis to physiological levels of copperSarah K Ward
Laboratory of Bacterial Genomics, Department of Pathobiological Sciences, University of Wisconsin Madison, 1656 Linden Drive, Madison, WI 53706 1581, USA
J Bacteriol 190:2939-46. 2008..In general, the Cu-induced transcriptome generated should help elucidate the role of the Cu response in maintaining M. tuberculosis survival during infection and could provide novel targets for controlling this virulent pathogen... 
Cloning, expression, purification and serodiagnostic evaluation of fourteen Mycobacterium paratuberculosis proteinsDonghee Cho
School of Veterinary Medicine, University of Wisconsin Madison, 2015 Linden Drive, Madison, WI 53706, USA
Protein Expr Purif 53:411-20. 2007..Taken together, our results showed that the natural forms of the identified proteins may be useful for diagnosis of bovine paratuberculosis...- Transcriptional profiling of mycobacterium tuberculosis during infection: lessons learnedSarah K Ward
Department of Pathobiological Sciences, University of Wisconsin Madison Madison, WI, USA
Front Microbiol 1:121. 2010..tuberculosis pathogenesis. Further, these data suggest that in vivo transcriptional profiling is a valid approach to identify genes required for bacterial pathogenesis... - Identification of novel virulence determinants in Mycobacterium paratuberculosis by screening a library of insertional mutantsSung Jae Shin
Department of Animal Health and Biomedical Sciences, University of Wisconsin-Madison, 1656 Linden Drive, Madison, WI 53706-1581, USA
Infect Immun 74:3825-33. 2006.... 
Single-Cell Elemental Analysis of Bacteria: Quantitative Analysis of Polyphosphates in Mycobacterium tuberculosisSarah K Ward
Department of Pathobiological Sciences, University of Wisconsin Madison Madison, WI, USA
Front Cell Infect Microbiol 2:63. 2012..tuberculosis, with potential future applications for in vivo analysis...- Genome-wide analysis of the emerging infection with Mycobacterium avium subspecies paratuberculosis in the Arabian camels (Camelus dromedarius)Pallab Ghosh
Department of Pathobiological Sciences, University of Wisconsin Madison, Madison, Wisconsin, United States of America
PLoS ONE 7:e31947. 2012..The sequenced genomes of M. ap isolates from camels will further assist our efforts to understand JD pathogenesis and the dynamic of disease transmission across animal species... - Genome-Wide Sequence Variation among Mycobacterium avium Subspecies paratuberculosis Isolates: A Better Understanding of Johne's Disease Transmission DynamicsChung Yi Hsu
Laboratory of Bacterial Genomics, Department of Pathobiological Sciences, University of Wisconsin Madison Madison, WI, USA
Front Microbiol 2:236. 2011..Genome-wide analysis described here could provide a strong foundation for a population genetic structure that could be useful for the analysis of mycobacterial evolution and for the tracking of Johne's disease transmission among animals... - Experimental challenge models for Johne's disease: a review and proposed international guidelinesMurray E Hines
Tifton Veterinary Diagnostic and Investigational Laboratory, University of Georgia, Tifton, GA 31793, USA
Vet Microbiol 122:197-222. 2007..paratuberculosis used, challenge dose, dose frequency, age of challenge, route of challenge, preparation of inoculum, experimental animal selection, quality control, minimal experimental endpoints and other parameters... - CsoR is a novel Mycobacterium tuberculosis copper-sensing transcriptional regulatorTong Liu
Department of Biochemistry and Biophysics, Texas A and M University, College Station, Texas 77843 2128, USA
Nat Chem Biol 3:60-8. 2007..Substitution of Cys36 or His61 with alanine abolishes Cu(I)- and CsoR-dependent regulation in vivo and in vitro. Potential roles of CsoR in Mtb pathogenesis are discussed... - The temporal expression profile of Mycobacterium tuberculosis infection in miceAdel M Talaat
Center for Biomedical Inventions, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390-8573, USA
Proc Natl Acad Sci U S A 101:4602-7. 2004..The in vivo expression-profiling technology presented should be applicable to any microbial model of infection... - Genomic DNA standards for gene expression profiling in Mycobacterium tuberculosisAdel M Talaat
Center for Biomedical Inventions and Department of Medicine, University of Texas-Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390-8573, USA
Nucleic Acids Res 30:e104. 2002..We conclude that genomic DNA standards offer advantages over conventional RNA normalization procedures and can be adapted for the investigation of microbial genomes... - Priming reverse transcription with oligo(dT) does not yield representative samples of Mycobacterium tuberculosis cDNADavid L Lakey
Center for Pulmonary and Infectious Disease Control, and Department of Microbiology, University of Texas Health Center, Tyler, TX 75708, USA
Microbiology 148:2567-72. 2002..tuberculosis microarray. These data demonstrate that priming of reverse transcription of mycobacterial mRNA with oligo(dT) does not yield representative samples of cDNA... - DNA microarray analysis of differential gene expression in Borrelia burgdorferi, the Lyme disease spirocheteAndrew T Revel
Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
Proc Natl Acad Sci U S A 99:1562-7. 2002..The microarray data thus provide a basis for formulating new testable hypotheses regarding the life cycle of Bb and attaining a more complete understanding of many aspects of Bb's complex parasitic strategies... - Identification of Mycobacterium marinum virulence genes using signature-tagged mutagenesis and the goldfish model of mycobacterial pathogenesisKristin M Ruley
Center for Vaccine Development, University of Maryland School of Medicine, Baltimore, MD 21201, USA
FEMS Microbiol Lett 232:75-81. 2004..DNA sequence analysis of these 40 mutants identified transposon insertions in 35 unique loci. Twenty-eight out of 33 (85%) loci encoding putative virulence genes have homologous genes in M. tuberculosis... - Shotgun library construction in a dayIrene T Rombel
Center for Biomedical Inventions, UT Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, TX 75390-9185, USA
Biotechniques 34:244-6, 248, 250. 2003
Research Grants
- In vivo Transcriptional Analysis of Latent TuberculosisADEL TALAAT; Fiscal Year: 2007..Transcriptional regulators that could contribute to both chronic and reactivation stages of tuberculosis will be the target for future analysis. ..