Adel M Talaat

Summary

Affiliation: University of Wisconsin
Country: USA

Publications

  1. pmc Mycobacterial bacilli are metabolically active during chronic tuberculosis in murine lungs: insights from genome-wide transcriptional profiling
    Adel M Talaat
    Laboratory of Bacterial Genomics, Department of Pathobiological Sciences, University of Wisconsin Madison, Madison, WI 53706 1581, USA
    J Bacteriol 189:4265-74. 2007
  2. pmc Transcriptional profiling of mycobacterium tuberculosis during infection: lessons learned
    Sarah K Ward
    Department of Pathobiological Sciences, University of Wisconsin Madison Madison, WI, USA
    Front Microbiol 1:121. 2010
  3. pmc Genome-Wide Sequence Variation among Mycobacterium avium Subspecies paratuberculosis Isolates: A Better Understanding of Johne's Disease Transmission Dynamics
    Chung Yi Hsu
    Laboratory of Bacterial Genomics, Department of Pathobiological Sciences, University of Wisconsin Madison Madison, WI, USA
    Front Microbiol 2:236. 2011
  4. pmc Genome sequencing of ovine isolates of Mycobacterium avium subspecies paratuberculosis offers insights into host association
    John P Bannantine
    National Animal Disease Center, USDA Agricultural Research Service, Ames, Iowa, USA
    BMC Genomics 13:89. 2012
  5. pmc Whole-genome plasticity among Mycobacterium avium subspecies: insights from comparative genomic hybridizations
    Chia Wei Wu
    Department of Animal Health and Biomedical Sciences, University of Wisconsin Madison, 1656 Linden Drive, Madison, WI 53706 1581
    J Bacteriol 188:711-23. 2006
  6. pmc Expression library immunization: a road map for discovery of vaccines against infectious diseases
    Adel M Talaat
    Department of Animal Health and Biomedical Sciences, University of Wisconsin Madison, 1656 Linden Drive, Madison, WI 53706 1581, USA
    Infect Immun 73:7089-98. 2005
  7. pmc mosR, a novel transcriptional regulator of hypoxia and virulence in Mycobacterium tuberculosis
    Bassam Abomoelak
    Department of Pathobiological Sciences, University of Wisconsin Madison, Madison, WI 53706, USA
    J Bacteriol 191:5941-52. 2009
  8. pmc CtpV: a putative copper exporter required for full virulence of Mycobacterium tuberculosis
    Sarah K Ward
    Department of Pathobiological Sciences, University of Wisconsin Madison, 1656 Linden Drive, Madison, WI 53706, USA
    Mol Microbiol 77:1096-110. 2010
  9. pmc A novel cell wall lipopeptide is important for biofilm formation and pathogenicity of Mycobacterium avium subspecies paratuberculosis
    Chia Wei Wu
    The Laboratory of Bacterial Genomics, Department of Pathobiological Sciences, University of Wisconsin Madison, 1656 Linden Drive, Madison, WI 53706 1581, USA
    Microb Pathog 46:222-30. 2009
  10. pmc Key role for the alternative sigma factor, SigH, in the intracellular life of Mycobacterium avium subsp. paratuberculosis during macrophage stress
    Pallab Ghosh
    Laboratory of Bacterial Genomics, Department of Pathobiological Sciences, University of Wisconsin Madison, Madison, Wisconsin, USA
    Infect Immun 81:2242-57. 2013

Research Grants

Collaborators

Detail Information

Publications33

  1. pmc Mycobacterial bacilli are metabolically active during chronic tuberculosis in murine lungs: insights from genome-wide transcriptional profiling
    Adel M Talaat
    Laboratory of Bacterial Genomics, Department of Pathobiological Sciences, University of Wisconsin Madison, Madison, WI 53706 1581, USA
    J Bacteriol 189:4265-74. 2007
    ..A similar approach can be adopted to study key stages of intracellular pathogens...
  2. pmc Transcriptional profiling of mycobacterium tuberculosis during infection: lessons learned
    Sarah K Ward
    Department of Pathobiological Sciences, University of Wisconsin Madison Madison, WI, USA
    Front Microbiol 1:121. 2010
    ..tuberculosis pathogenesis. Further, these data suggest that in vivo transcriptional profiling is a valid approach to identify genes required for bacterial pathogenesis...
  3. pmc Genome-Wide Sequence Variation among Mycobacterium avium Subspecies paratuberculosis Isolates: A Better Understanding of Johne's Disease Transmission Dynamics
    Chung Yi Hsu
    Laboratory of Bacterial Genomics, Department of Pathobiological Sciences, University of Wisconsin Madison Madison, WI, USA
    Front Microbiol 2:236. 2011
    ..Genome-wide analysis described here could provide a strong foundation for a population genetic structure that could be useful for the analysis of mycobacterial evolution and for the tracking of Johne's disease transmission among animals...
  4. pmc Genome sequencing of ovine isolates of Mycobacterium avium subspecies paratuberculosis offers insights into host association
    John P Bannantine
    National Animal Disease Center, USDA Agricultural Research Service, Ames, Iowa, USA
    BMC Genomics 13:89. 2012
    ..To systematically characterize all genomic differences that may be associated with the specific hosts, we sequenced the genomes of three U.S. sheep isolates and also obtained an optical map...
  5. pmc Whole-genome plasticity among Mycobacterium avium subspecies: insights from comparative genomic hybridizations
    Chia Wei Wu
    Department of Animal Health and Biomedical Sciences, University of Wisconsin Madison, 1656 Linden Drive, Madison, WI 53706 1581
    J Bacteriol 188:711-23. 2006
    ..The presented findings will affect the future design of diagnostics and vaccines for Johne's and Crohn's diseases and provide a model for genomic analysis of closely related bacteria...
  6. pmc Expression library immunization: a road map for discovery of vaccines against infectious diseases
    Adel M Talaat
    Department of Animal Health and Biomedical Sciences, University of Wisconsin Madison, 1656 Linden Drive, Madison, WI 53706 1581, USA
    Infect Immun 73:7089-98. 2005
  7. pmc mosR, a novel transcriptional regulator of hypoxia and virulence in Mycobacterium tuberculosis
    Bassam Abomoelak
    Department of Pathobiological Sciences, University of Wisconsin Madison, Madison, WI 53706, USA
    J Bacteriol 191:5941-52. 2009
    ....
  8. pmc CtpV: a putative copper exporter required for full virulence of Mycobacterium tuberculosis
    Sarah K Ward
    Department of Pathobiological Sciences, University of Wisconsin Madison, 1656 Linden Drive, Madison, WI 53706, USA
    Mol Microbiol 77:1096-110. 2010
    ..tuberculosis, supporting the hypothesis that copper response could be important to intracellular pathogens, in general...
  9. pmc A novel cell wall lipopeptide is important for biofilm formation and pathogenicity of Mycobacterium avium subspecies paratuberculosis
    Chia Wei Wu
    The Laboratory of Bacterial Genomics, Department of Pathobiological Sciences, University of Wisconsin Madison, 1656 Linden Drive, Madison, WI 53706 1581, USA
    Microb Pathog 46:222-30. 2009
    ..ap. Molecular tools to better control M. ap infections could be developed utilizing the presented findings...
  10. pmc Key role for the alternative sigma factor, SigH, in the intracellular life of Mycobacterium avium subsp. paratuberculosis during macrophage stress
    Pallab Ghosh
    Laboratory of Bacterial Genomics, Department of Pathobiological Sciences, University of Wisconsin Madison, Madison, Wisconsin, USA
    Infect Immun 81:2242-57. 2013
    ..avium subsp. paratuberculosis virulence. Such analysis could identify potential targets for further testing as vaccine candidates against Johne's disease...
  11. pmc Defining the stressome of Mycobacterium avium subsp. paratuberculosis in vitro and in naturally infected cows
    Chia Wei Wu
    Laboratory of Bacterial Genomics, Department of Pathobiological Sciences, University of Wisconsin Madison, 1656 Linden Drive, Madison, WI 53706 1581, USA
    J Bacteriol 189:7877-86. 2007
    ..Overall, the employed approach profiled mycobacterial genetic networks triggered by variable stressors and identified a novel set of putative virulence genes. A similar approach could be applied to analyze other intracellular pathogens...
  12. pmc Characterization of a novel heat shock protein (Hsp22.5) involved in the pathogenesis of Mycobacterium tuberculosis
    Bassam Abomoelak
    Department of Pathobiological Sciences, University of Wisconsin Madison, 1656 Linden Drive, Madison, Wisconsin 53706 1581, USA
    J Bacteriol 193:3497-505. 2011
    ..Overall, we suggest that rv0990c encodes a heat shock protein that plays an important role in mycobacterial virulence. Hence, we renamed rv0990c heat shock protein 22.5 (hsp22.5), reflecting its molecular mass...
  13. pmc Invasion and persistence of Mycobacterium avium subsp. paratuberculosis during early stages of Johne's disease in calves
    Chia Wei Wu
    The Laboratory of Bacterial Genomics, Department of Pathobiological Sciences, University of Wisconsin Madison, 1656 Linden Drive, Madison, WI 53706 1581, USA
    Infect Immun 75:2110-9. 2007
    ..Overall, novel insights into the early stages of Johne's disease were obtained, and a practical model of mycobacterial invasiveness was developed. A similar approach can be used for other enteric bacteria...
  14. pmc Optical mapping of the Mycobacterium avium subspecies paratuberculosis genome
    Chia Wei Wu
    The Laboratory of Bacterial Genomics, Department of Pathobiological Sciences, University of Wisconsin Madison, WI, USA
    BMC Genomics 10:25. 2009
    ..In an effort to better examine diversity among M. ap strains, we used optical mapping to profile genomic variations between strains of M. ap K-10 (sequenced strain) and M. ap ATCC 19698 (type strain)...
  15. pmc The global responses of Mycobacterium tuberculosis to physiological levels of copper
    Sarah K Ward
    Laboratory of Bacterial Genomics, Department of Pathobiological Sciences, University of Wisconsin Madison, 1656 Linden Drive, Madison, WI 53706 1581, USA
    J Bacteriol 190:2939-46. 2008
    ..In general, the Cu-induced transcriptome generated should help elucidate the role of the Cu response in maintaining M. tuberculosis survival during infection and could provide novel targets for controlling this virulent pathogen...
  16. ncbi request reprint Cloning, expression, purification and serodiagnostic evaluation of fourteen Mycobacterium paratuberculosis proteins
    Donghee Cho
    School of Veterinary Medicine, University of Wisconsin Madison, 2015 Linden Drive, Madison, WI 53706, USA
    Protein Expr Purif 53:411-20. 2007
    ..Taken together, our results showed that the natural forms of the identified proteins may be useful for diagnosis of bovine paratuberculosis...
  17. pmc Protection by novel vaccine candidates, Mycobacterium tuberculosis ΔmosR and ΔechA7, against challenge with a Mycobacterium tuberculosis Beijing strain
    Sarah A Marcus
    Department of Pathobiological Sciences, University of Wisconsin Madison, Madison, WI 53706, USA Electronic address
    Vaccine 33:5633-9. 2015
    ..Although these strains require further development to meet safety standards, this first evidence of protection by these two new, live attenuated vaccine candidates shows promise. ..
  18. pmc CsoR Is Essential for Maintaining Copper Homeostasis in Mycobacterium tuberculosis
    Sarah A Marcus
    Department of Pathobiological Sciences, University of Wisconsin Madison, Madison, Wisconsin, United States of America
    PLoS ONE 11:e0151816. 2016
    ..tuberculosis fitness within the host. Overall, analysis of CsoR provided an increased understanding of the M. tuberculosis copper response with implications for other intracellular pathogens harboring CsoR. ..
  19. pmc Single-cell elemental analysis of bacteria: quantitative analysis of polyphosphates in Mycobacterium tuberculosis
    Sarah K Ward
    Department of Pathobiological Sciences, University of Wisconsin Madison Madison, WI, USA
    Front Cell Infect Microbiol 2:63. 2012
    ..tuberculosis, with potential future applications for in vivo analysis...
  20. pmc Identification of novel virulence determinants in Mycobacterium paratuberculosis by screening a library of insertional mutants
    Sung Jae Shin
    Department of Animal Health and Biomedical Sciences, University of Wisconsin Madison, 1656 Linden Drive, Madison, WI 53706 1581, USA
    Infect Immun 74:3825-33. 2006
    ....
  21. pmc Virulence and immunity orchestrated by the global gene regulator sigL in Mycobacterium avium subsp. paratuberculosis
    Pallab Ghosh
    Department of Pathobiological Sciences, University of Wisconsin Madison, Madison, Wisconsin, USA
    Infect Immun 82:3066-75. 2014
    ..Overall, our study highlights critical role of sigL in the pathogenesis and immunity of M. avium subsp. paratuberculosis infection, a potential role that could be shared by similar proteins in other intracellular pathogens. ..
  22. doi request reprint Superior protection elicited by live-attenuated vaccines in the murine model of paratuberculosis
    Pallab Ghosh
    Department of Pathobiological Sciences, University of Wisconsin Madison, Madison, WI, USA Electronic address
    Vaccine 33:7262-70. 2015
    ..Overall, our approach provides a rational basis for using live-attenuated mutant strains to develop improved vaccines that elicit robust immunity against this chronic infection. ..
  23. pmc Virtual Screening for UDP-Galactopyranose Mutase Ligands Identifies a New Class of Antimycobacterial Agents
    Virginia A Kincaid
    Department of Biochemistry, University of Wisconsin Madison, Madison, Wisconsin 53706, United States
    ACS Chem Biol 10:2209-18. 2015
    ..By focusing on promising compounds, the virtual screen rescued false negatives, providing a blueprint for generating new UGM probes and therapeutic leads. ..
  24. pmc Genome-wide analysis of the emerging infection with Mycobacterium avium subspecies paratuberculosis in the Arabian camels (Camelus dromedarius)
    Pallab Ghosh
    Department of Pathobiological Sciences, University of Wisconsin Madison, Madison, Wisconsin, United States of America
    PLoS ONE 7:e31947. 2012
    ..The sequenced genomes of M. ap isolates from camels will further assist our efforts to understand JD pathogenesis and the dynamic of disease transmission across animal species...
  25. pmc A murine oral model for Mycobacterium avium subsp. paratuberculosis infection and immunomodulation with Lactobacillus casei ATCC 334
    Meagan A Cooney
    Laboratory of Bacterial Genomics, Department of Pathobiological Sciences, University of Wisconsin Madison Madison, WI, USA
    Front Cell Infect Microbiol 4:11. 2014
    ..Although the L. casei ATCC 334 was not a promising candidate for controlling paratuberculosis, we established a protocol to screen other probiotic candidates. ..
  26. ncbi request reprint CsoR is a novel Mycobacterium tuberculosis copper-sensing transcriptional regulator
    Tong Liu
    Department of Biochemistry and Biophysics, Texas A and M University, College Station, Texas 77843 2128, USA
    Nat Chem Biol 3:60-8. 2007
    ..Substitution of Cys36 or His61 with alanine abolishes Cu(I)- and CsoR-dependent regulation in vivo and in vitro. Potential roles of CsoR in Mtb pathogenesis are discussed...
  27. ncbi request reprint Priming reverse transcription with oligo(dT) does not yield representative samples of Mycobacterium tuberculosis cDNA
    David L Lakey
    Center for Pulmonary and Infectious Disease Control, and Department of Microbiology, University of Texas Health Center, Tyler, TX 75708, USA
    Microbiology 148:2567-72. 2002
    ..tuberculosis microarray. These data demonstrate that priming of reverse transcription of mycobacterial mRNA with oligo(dT) does not yield representative samples of cDNA...
  28. pmc Genomic DNA standards for gene expression profiling in Mycobacterium tuberculosis
    Adel M Talaat
    Center for Biomedical Inventions and Department of Medicine, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390 8573, USA
    Nucleic Acids Res 30:e104. 2002
    ..We conclude that genomic DNA standards offer advantages over conventional RNA normalization procedures and can be adapted for the investigation of microbial genomes...
  29. ncbi request reprint Experimental challenge models for Johne's disease: a review and proposed international guidelines
    Murray E Hines
    Tifton Veterinary Diagnostic and Investigational Laboratory, University of Georgia, Tifton, GA 31793, USA
    Vet Microbiol 122:197-222. 2007
    ..paratuberculosis used, challenge dose, dose frequency, age of challenge, route of challenge, preparation of inoculum, experimental animal selection, quality control, minimal experimental endpoints and other parameters...
  30. pmc The temporal expression profile of Mycobacterium tuberculosis infection in mice
    Adel M Talaat
    Center for Biomedical Inventions, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390 8573, USA
    Proc Natl Acad Sci U S A 101:4602-7. 2004
    ..The in vivo expression-profiling technology presented should be applicable to any microbial model of infection...
  31. pmc DNA microarray analysis of differential gene expression in Borrelia burgdorferi, the Lyme disease spirochete
    Andrew T Revel
    Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    Proc Natl Acad Sci U S A 99:1562-7. 2002
    ..The microarray data thus provide a basis for formulating new testable hypotheses regarding the life cycle of Bb and attaining a more complete understanding of many aspects of Bb's complex parasitic strategies...
  32. ncbi request reprint Shotgun library construction in a day
    Irene T Rombel
    Center for Biomedical Inventions, UT Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, TX 75390 9185, USA
    Biotechniques 34:244-6, 248, 250. 2003
  33. ncbi request reprint Identification of Mycobacterium marinum virulence genes using signature-tagged mutagenesis and the goldfish model of mycobacterial pathogenesis
    Kristin M Ruley
    Center for Vaccine Development, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    FEMS Microbiol Lett 232:75-81. 2004
    ..DNA sequence analysis of these 40 mutants identified transposon insertions in 35 unique loci. Twenty-eight out of 33 (85%) loci encoding putative virulence genes have homologous genes in M. tuberculosis...

Research Grants1

  1. In vivo Transcriptional Analysis of Latent Tuberculosis
    ADEL TALAAT; Fiscal Year: 2008
    ..Transcriptional regulators that could contribute to both chronic and reactivation stages of tuberculosis will be the target for future analysis. [unreadable] [unreadable] [unreadable] [unreadable]..