Jonathan Tait

Summary

Affiliation: University of Washington
Country: USA

Publications

  1. pmc Transmembrane voltage regulates binding of annexin V and lactadherin to cells with exposed phosphatidylserine
    Christina Smith
    Department of Laboratory Medicine, University of Washington, Seattle, WA 98195 7110, USA
    BMC Biochem 10:5. 2009
  2. doi request reprint Imaging of apoptosis
    Jonathan F Tait
    Department of Laboratory Medicine, University of Washington, Seattle, WA 98195 7110, USA
    J Nucl Med 49:1573-6. 2008
  3. ncbi request reprint Improved detection of cell death in vivo with annexin V radiolabeled by site-specific methods
    Jonathan F Tait
    Department of Laboratory Medicine, University of Washington, Seattle, 98195 7110, USA
    J Nucl Med 47:1546-53. 2006
  4. pmc Structural requirements for in vivo detection of cell death with 99mTc-annexin V
    Jonathan F Tait
    Department of Laboratory Medicine, University of Washington, Seattle, WA 98195 7110, USA
    J Nucl Med 46:807-15. 2005
  5. ncbi request reprint Phosphatidylserine receptors: role of CD36 in binding of anionic phospholipid vesicles to monocytic cells
    J F Tait
    Department of Laboratory Medicine, University of Washington, Seattle, Washington 98195 7110, USA
    J Biol Chem 274:3048-54. 1999
  6. ncbi request reprint Measurement of the affinity and cooperativity of annexin V-membrane binding under conditions of low membrane occupancy
    Jonathan F Tait
    Department of Laboratory Medicine, Medicine Medical Genetics, University of Washington, Box 357110 Seattle, WA 98195, USA
    Anal Biochem 329:112-9. 2004
  7. ncbi request reprint Measurement of phosphatidylserine exposure in leukocytes and platelets by whole-blood flow cytometry with annexin V
    J F Tait
    Department of Laboratory Medicine, University of Washington, Seattle, WA 98195 7110, USA
    Blood Cells Mol Dis 25:271-8. 1999
  8. ncbi request reprint Development and characterization of annexin V mutants with endogenous chelation sites for (99m)Tc
    J F Tait
    Departments of Laboratory Medicine, Medicine Medical Genetics and Pathology, University of Washington, Seattle, Washington 98195 7110, USA
    Bioconjug Chem 11:918-25. 2000
  9. ncbi request reprint Development of annexin V mutants suitable for labeling with Tc(i)-carbonyl complex
    Jonathan F Tait
    Department of Laboratory Medicine, University of Washington, Seattle, Washington 98195 7110, USA
    Bioconjug Chem 13:1119-23. 2002
  10. ncbi request reprint The preparation and phospholipid binding property of the C2 domain of human factor VIII
    Kazuya Takeshima
    Department of Biochemistry, University of Washington, Seattle Washington 98195, USA
    Thromb Haemost 89:788-94. 2003

Research Grants

Collaborators

Detail Information

Publications30

  1. pmc Transmembrane voltage regulates binding of annexin V and lactadherin to cells with exposed phosphatidylserine
    Christina Smith
    Department of Laboratory Medicine, University of Washington, Seattle, WA 98195 7110, USA
    BMC Biochem 10:5. 2009
    ..Cells expose phosphatidylserine during apoptosis. The voltage across the plasma membrane also decreases or disappears during apoptosis, but the physiological significance of this is unknown...
  2. doi request reprint Imaging of apoptosis
    Jonathan F Tait
    Department of Laboratory Medicine, University of Washington, Seattle, WA 98195 7110, USA
    J Nucl Med 49:1573-6. 2008
    ....
  3. ncbi request reprint Improved detection of cell death in vivo with annexin V radiolabeled by site-specific methods
    Jonathan F Tait
    Department of Laboratory Medicine, University of Washington, Seattle, 98195 7110, USA
    J Nucl Med 47:1546-53. 2006
    ..We now show that this assumption is incorrect by measuring the affinity of annexin V for cells in vitro by quantitative calcium titration under conditions of low membrane occupancy...
  4. pmc Structural requirements for in vivo detection of cell death with 99mTc-annexin V
    Jonathan F Tait
    Department of Laboratory Medicine, University of Washington, Seattle, WA 98195 7110, USA
    J Nucl Med 46:807-15. 2005
    ..We investigated how changes in membrane-binding affinity, molecular charge, and method of labeling affected its biodistribution in normal mice and its uptake in apoptotic tissues...
  5. ncbi request reprint Phosphatidylserine receptors: role of CD36 in binding of anionic phospholipid vesicles to monocytic cells
    J F Tait
    Department of Laboratory Medicine, University of Washington, Seattle, Washington 98195 7110, USA
    J Biol Chem 274:3048-54. 1999
    ..In addition, CD36 appears to be specific for PtdSer among anionic phospholipids, and non-phospholipid ligands of CD36 do not share binding sites with PtdSer on CD36...
  6. ncbi request reprint Measurement of the affinity and cooperativity of annexin V-membrane binding under conditions of low membrane occupancy
    Jonathan F Tait
    Department of Laboratory Medicine, Medicine Medical Genetics, University of Washington, Box 357110 Seattle, WA 98195, USA
    Anal Biochem 329:112-9. 2004
    ..This method allows the accurate determination of the affinity and cooperativity of annexin V-membrane binding and will be useful for the evaluation of modified annexin V derivatives intended for diagnostic and therapeutic applications...
  7. ncbi request reprint Measurement of phosphatidylserine exposure in leukocytes and platelets by whole-blood flow cytometry with annexin V
    J F Tait
    Department of Laboratory Medicine, University of Washington, Seattle, WA 98195 7110, USA
    Blood Cells Mol Dis 25:271-8. 1999
    ..Whole-blood flow cytometry with annexin V can reliably measure the state of PS exposure in platelets and leukocytes, and the results confirm that these cell..
  8. ncbi request reprint Development and characterization of annexin V mutants with endogenous chelation sites for (99m)Tc
    J F Tait
    Departments of Laboratory Medicine, Medicine Medical Genetics and Pathology, University of Washington, Seattle, Washington 98195 7110, USA
    Bioconjug Chem 11:918-25. 2000
    ..These annexin V derivatives may be useful for in vivo imaging of cell death...
  9. ncbi request reprint Development of annexin V mutants suitable for labeling with Tc(i)-carbonyl complex
    Jonathan F Tait
    Department of Laboratory Medicine, University of Washington, Seattle, Washington 98195 7110, USA
    Bioconjug Chem 13:1119-23. 2002
    ..In conclusion, annexin V can be modified near its N-terminus to incorporate sequences that form specific chelation sites for (99m)Tc-carbonyl without altering its high affinity for cell membranes...
  10. ncbi request reprint The preparation and phospholipid binding property of the C2 domain of human factor VIII
    Kazuya Takeshima
    Department of Biochemistry, University of Washington, Seattle Washington 98195, USA
    Thromb Haemost 89:788-94. 2003
    ....
  11. ncbi request reprint Essential role of B-helix calcium binding sites in annexin V-membrane binding
    Ming Jin
    Department of Laboratory Medicine, University of Washington, Seattle, Washington 98195, USA
    J Biol Chem 279:40351-7. 2004
    ..This study shows an essential and previously unappreciated role for B-helix calcium binding sites in the membrane binding of annexins and indicates that all four domains of the molecule are required for maximum membrane binding affinity...
  12. pmc Site-specific labeling of annexin V with F-18 for apoptosis imaging
    Xuehe Li
    Department of Radiology, University of Washington Medical Center, Seattle, Washington 98195, USA
    Bioconjug Chem 19:1684-8. 2008
    ..Erythrocyte binding assay of [(18)F]FAN-128 showed that this modification of annexin V-128 did not compromise its membrane binding affinity. Thus, an in vivo investigation of [ (18)F]FAN-128 as an apoptosis imaging agent is warranted...
  13. doi request reprint Entropic and enthalpic contributions to annexin V-membrane binding: a comprehensive quantitative model
    Brian Jeppesen
    Department of Laboratory Medicine, University of Washington, Seattle, WA 98195, USA
    J Biol Chem 283:6126-35. 2008
    ..Dehydration of hydrophobic regions of the protein surface as they enter the interfacial region makes an important contribution to overall binding affinity, supplementing the role of protein-calcium-phosphate chelates...
  14. ncbi request reprint Evaluation of 18F-annexin V as a PET imaging agent in an animal model of apoptosis
    Kevin J Yagle
    Department of Radiology, Molecular Imaging Laboratory, University of Washington, Seattle, Washington 98195, USA
    J Nucl Med 46:658-66. 2005
    ..Because one of the earliest measurable events in apoptosis is the eversion of phosphatidylserine from the inner membrane leaflet to the outer cell surface, annexin V has proven useful for detecting the earliest stages of apoptosis...
  15. ncbi request reprint Production of [F-18]fluoroannexin for imaging apoptosis with PET
    John R Grierson
    Department of Radiology, University of Washington, Seattle, Washington 91895, USA
    Bioconjug Chem 15:373-9. 2004
    ..F-18]Fluoroannexin should prove useful in imaging targeted apoptosis...
  16. ncbi request reprint 18F-labelled annexin V: a PET tracer for apoptosis imaging
    Yoshihiro Murakami
    The Medical and Pharmacological Research Center Foundation, Wo32, 925 0613, Inoyama, Hakui, Ishikawa, Japan
    Eur J Nucl Med Mol Imaging 31:469-74. 2004
    ..The low uptake of [18F]annexin V in these organs might represent an advantage over [(99m)Tc]annexin V...
  17. ncbi request reprint Feasibility of 99mTc-annexin V for repetitive detection of apoptotic tumor response to chemotherapy: an experimental study using a rat tumor model
    Yuji Kuge
    Department of Tracer Kinetics, Graduate School of Medicine, Hokkaido University, Kita15 Nishi7, Kita ku, Sapporo 060 8638, Japan
    J Nucl Med 45:309-12. 2004
    ..To determine whether there is interference with repetitive doses of annexin V, we evaluated the effects of previous administration of cold annexin V on accumulation of (99m)Tc-annexin V in tumors in an experimental tumor model...
  18. ncbi request reprint Detection of focal hypoxic-ischemic injury and neuronal stress in a rodent model of unilateral MCA occlusion/reperfusion using radiolabeled annexin V
    Carina Mari
    Division of Nuclear Medicine, Department of Radiology, Stanford University Hospital, Stanford, CA 94305, USA
    Eur J Nucl Med Mol Imaging 31:733-9. 2004
    ..This pattern suggests that neuronal stress may play a role in the response of the brain to focal injury and be responsible for annexin V uptake outside the region of ischemic insult...
  19. ncbi request reprint Effect of postconditioning on myocardial 99mTc-annexin-V uptake: comparison with ischemic preconditioning and caspase inhibitor treatment
    Junichi Taki
    Department of Biotracer Medicine, Kanazawa University Graduate School of Medical Sciences, Kanazawa, Japan
    J Nucl Med 48:1301-7. 2007
    ....
  20. ncbi request reprint Radiolabeling of HYNIC-annexin V with technetium-99m for in vivo imaging of apoptosis
    Francis G Blankenberg
    Department of Radiology, Stanford University, Palo Alto, California 94305, USA
    Nat Protoc 1:108-10. 2006
    ..Once prepared, HYNIC-annexin V can be labeled with 99mTc, a widely available gamma-radiation-emitting radionuclide, for intravenous injection in as little as 30 min without the need for specialized reagents or equipment...
  21. ncbi request reprint Nuclear medicine in the era of genomics and proteomics: lessons from annexin V
    Tarik Z Belhocine
    Department of Nuclear Medicine, Jules Bordet Cancer Institute, Brussels, Belgium
    J Proteome Res 3:345-9. 2004
    ..This model also emphasizes the value of synergy between basic and medical specialties in developing and optimizing a clinically useful product initially derived from basic investigation...
  22. ncbi request reprint Evaluation of 99mTc-MAG3-annexin V: influence of the chelate on in vitro and in vivo properties in mice
    Jean Luc Vanderheyden
    Department of Radiology, University of Massachusetts Medical School, Worcester, 01655, USA
    Nucl Med Biol 33:135-44. 2006
    ..Nevertheless, (99m)Tc MAG(3)-annexin may be an attractive alternative to (99m)Tc Hynic-annexin for the in vivo imaging of phosphatidylserine receptors...
  23. ncbi request reprint Detection of apoptotic tumor response in vivo after a single dose of chemotherapy with 99mTc-annexin V
    Takafumi Mochizuki
    Department of Radiology, Nikko Memorial Hospital, Muroran, Japan
    J Nucl Med 44:92-7. 2003
    ..To determine the effectiveness of imaging with this agent as a reflection of the degree of apoptosis after the first dose of chemotherapy, we studied rats with an engrafted hepatoma...
  24. ncbi request reprint Enhanced apoptotic reaction correlates with suppressed tumor glucose utilization after cytotoxic chemotherapy: use of 99mTc-Annexin V, 18F-FDG, and histologic evaluation
    Toshiki Takei
    Department of Nuclear Medicine, Graduate School of Medicine, Hokkaido University, Kita 15 Nishi 7, Kita ku, Sapporo 060 8638, Japan
    J Nucl Med 46:794-9. 2005
    ..We determined the relationship between apoptosis and glucose utilization during cancer chemotherapy using (99m)Tc-annexin V ((99m)Tc-annexin A5) and (18)F-FDG and compared their uptake with histologic findings in a rat tumor model...
  25. ncbi request reprint Multi-modality imaging identifies key times for annexin V imaging as an early predictor of therapeutic outcome
    Stefanie J Mandl
    Stanford University, School of Medicine, CA 94305 5208, USA
    Mol Imaging 3:1-8. 2004
    ..Multimodality imaging revealed the temporal patterns of tumor cell loss and annexin V uptake revealing a better understanding of the timing of radiolabeled annexin V uptake for its development as a marker of therapeutic efficacy...
  26. ncbi request reprint Detection of cardiomyocyte death in a rat model of ischemia and reperfusion using 99mTc-labeled annexin V
    Junichi Taki
    Department of Biotracer Medicine, Kanazawa University Graduate School of Medical Sciences, Kanazawa, Japan
    J Nucl Med 45:1536-41. 2004
    ..To determine the time course, location, and extent of this process, we studied groups of rats after a 20-min interval of coronary occlusion and reperfusion...
  27. ncbi request reprint 99mTc-Hynic-annexin V imaging to evaluate inflammation and apoptosis in rats with autoimmune myocarditis
    Naoki Tokita
    Department of Internal Medicine, Kitasato University School of Medicine, 1 15 1 Kitasato, Sagamihara City, Kanagawa 228 8555, Japan
    Eur J Nucl Med Mol Imaging 30:232-8. 2003
    ..These changes are mirrored by the localization of (14)C-DG and (99m)Tc-annexin. Sites of inflammation and zones of apoptotic cell death change over the course of immune myocarditis...
  28. ncbi request reprint (99m)Tc-Annexin-V uptake in a rat model of variable ischemic severity and reperfusion time
    Junichi Taki
    Department of Biotracer Medicine, Kanazawa University Graduate School of Medical Sciences, Kanazawa, Japan
    Circ J 71:1141-6. 2007
    ....
  29. ncbi request reprint Time course of apoptotic tumor response after a single dose of chemotherapy: comparison with 99mTc-annexin V uptake and histologic findings in an experimental model
    Toshiki Takei
    Department of Nuclear Medicine, Graduate School of Medicine, Hokkaido University, Sapporo 060 8638, Japan
    J Nucl Med 45:2083-7. 2004
    ....
  30. ncbi request reprint Imaging cell death with radiolabeled annexin V in an experimental model of rheumatoid arthritis
    Anneke M Post
    Division of Nuclear Medicine, Department of Radiology, Stanford University, Stanford, California, USA
    J Nucl Med 43:1359-65. 2002
    ..002). CONCLUSION: 99mTc-annexin V can detect collagen-induced immune arthritis and its response to steroid therapy before joint destruction...

Research Grants4

  1. Membrane Binding and Imaging Applications of Annexins
    Jonathan Tait; Fiscal Year: 2007
    ..Determine which mutant proteins have faster removal from the target site, thus allowing for repeated measurement of PS expression over short periods of time (up to one day). ..