James Stull

Summary

Affiliation: University of Texas Southwestern Medical Center
Country: USA

Publications

  1. pmc Signalling to contractile proteins by muscarinic and purinergic pathways in neurally stimulated bladder smooth muscle
    Ming Ho Tsai
    Department of Physiology, UT Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, TX 75390 9040, USA
    J Physiol 590:5107-21. 2012
  2. pmc Myosin light chain kinase and the role of myosin light chain phosphorylation in skeletal muscle
    James T Stull
    Department of Physiology, University of Texas Southwestern Medical Center, Dallas, 75390 9040, USA
    Arch Biochem Biophys 510:120-8. 2011
  3. ncbi request reprint Quantitative measurements of Ca(2+)/calmodulin binding and activation of myosin light chain kinase in cells
    Ramaz Geguchadze
    Department of Physiology, UT Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, TX 75390 9040, USA
    FEBS Lett 557:121-4. 2004
  4. ncbi request reprint Myosin phosphatase targeting subunit 1 affects cell migration by regulating myosin phosphorylation and actin assembly
    Donglan Xia
    Department of Physiology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390 9040, USA
    Exp Cell Res 304:506-17. 2005
  5. pmc Myosin light chain kinase is central to smooth muscle contraction and required for gastrointestinal motility in mice
    Wei Qi He
    Model Animal Research Center and MOE Key Laboratory of Model Animal for Disease Study, Nanjing University, Nanjing, China
    Gastroenterology 135:610-20. 2008
  6. pmc Myosin light chain kinase activation and calcium sensitization in smooth muscle in vivo
    Yusuke Mizuno
    Dept Physiology, UT Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, TX 75390 9040, USA
    Am J Physiol Cell Physiol 295:C358-64. 2008
  7. pmc Myosin regulatory light chain phosphorylation attenuates cardiac hypertrophy
    Jian Huang
    Department of Physiology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    J Biol Chem 283:19748-56. 2008
  8. ncbi request reprint Enhanced skeletal muscle contraction with myosin light chain phosphorylation by a calmodulin-sensing kinase
    Jeffrey W Ryder
    Department of Physiology, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390, USA
    J Biol Chem 282:20447-54. 2007
  9. ncbi request reprint Properties of long myosin light chain kinase binding to F-actin in vitro and in vivo
    Lula Smith
    Department of Physiology, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9040, USA
    J Biol Chem 277:35597-604. 2002
  10. pmc Myosin light chain kinase and myosin phosphorylation effect frequency-dependent potentiation of skeletal muscle contraction
    Gang Zhi
    Department of Physiology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    Proc Natl Acad Sci U S A 102:17519-24. 2005

Research Grants

Collaborators

Detail Information

Publications13

  1. pmc Signalling to contractile proteins by muscarinic and purinergic pathways in neurally stimulated bladder smooth muscle
    Ming Ho Tsai
    Department of Physiology, UT Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, TX 75390 9040, USA
    J Physiol 590:5107-21. 2012
    ..Activation of muscarinic receptors recruits CPI-17, but not MYPT1-mediated Ca2+ sensitization. Furthermore, nerve-released ACh also initiates signalling cascades leading to phosphorylation-dependent desensitization of MLCK...
  2. pmc Myosin light chain kinase and the role of myosin light chain phosphorylation in skeletal muscle
    James T Stull
    Department of Physiology, University of Texas Southwestern Medical Center, Dallas, 75390 9040, USA
    Arch Biochem Biophys 510:120-8. 2011
    ..Moreover, RLC phosphorylation-mediated enhancements may interact with neural strategies for human skeletal muscle activation to ameliorate either central or peripheral aspects of fatigue...
  3. ncbi request reprint Quantitative measurements of Ca(2+)/calmodulin binding and activation of myosin light chain kinase in cells
    Ramaz Geguchadze
    Department of Physiology, UT Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, TX 75390 9040, USA
    FEBS Lett 557:121-4. 2004
    ..There was no evidence for CaM binding but not activating MLCK at low [Ca(2+)](i). At saturating [Ca(2+)](i) MLCK was not fully activated probably due to limited availability of cellular Ca(2+)/CaM...
  4. ncbi request reprint Myosin phosphatase targeting subunit 1 affects cell migration by regulating myosin phosphorylation and actin assembly
    Donglan Xia
    Department of Physiology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390 9040, USA
    Exp Cell Res 304:506-17. 2005
    ..Proper expression of MYPT1 or variant 2 is critical for RLC phosphorylation and actin assembly, thus maintaining normal cellular functions by simultaneously controlling cytoskeletal architecture and actomyosin activation...
  5. pmc Myosin light chain kinase is central to smooth muscle contraction and required for gastrointestinal motility in mice
    Wei Qi He
    Model Animal Research Center and MOE Key Laboratory of Model Animal for Disease Study, Nanjing University, Nanjing, China
    Gastroenterology 135:610-20. 2008
    ..We generated tamoxifen-inducible and smooth muscle-specific MLCK knockout (KO) mice and provide direct loss-of-function evidence that shows the primary importance of MLCK in phasic smooth muscle contractions...
  6. pmc Myosin light chain kinase activation and calcium sensitization in smooth muscle in vivo
    Yusuke Mizuno
    Dept Physiology, UT Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, TX 75390 9040, USA
    Am J Physiol Cell Physiol 295:C358-64. 2008
    ..Thus the onset of agonist-induced contraction in phasic smooth muscle results from the rapid and coordinated activation of MLCK with hierarchical inhibition of MLCP by CPI-17 and MYPT1 phosphorylation...
  7. pmc Myosin regulatory light chain phosphorylation attenuates cardiac hypertrophy
    Jian Huang
    Department of Physiology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    J Biol Chem 283:19748-56. 2008
    ..Thus, increased myosin RLC phosphorylation per se does not cause cardiac hypertrophy and probably inhibits physiological and pathophysiological hypertrophy by contributing to enhanced contractile performance and efficiency...
  8. ncbi request reprint Enhanced skeletal muscle contraction with myosin light chain phosphorylation by a calmodulin-sensing kinase
    Jeffrey W Ryder
    Department of Physiology, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390, USA
    J Biol Chem 282:20447-54. 2007
    ..Importantly, RLC phosphorylation plays a prominent role in skeletal muscle force potentiation of fast-twitch type IIb but not type I or IIa fibers...
  9. ncbi request reprint Properties of long myosin light chain kinase binding to F-actin in vitro and in vivo
    Lula Smith
    Department of Physiology, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9040, USA
    J Biol Chem 277:35597-604. 2002
    ..Whereas DFRXXL motifs are necessary and sufficient for short MLCK binding to actin-containing filaments, the DFRXXL motifs and the N-terminal extension of long MLCK confer high affinity binding to stress fibers in cells...
  10. pmc Myosin light chain kinase and myosin phosphorylation effect frequency-dependent potentiation of skeletal muscle contraction
    Gang Zhi
    Department of Physiology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    Proc Natl Acad Sci U S A 102:17519-24. 2005
    ....
  11. pmc Real-time evaluation of myosin light chain kinase activation in smooth muscle tissues from a transgenic calmodulin-biosensor mouse
    Eiji Isotani
    Department of Physiology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390 9040, USA
    Proc Natl Acad Sci U S A 101:6279-84. 2004
    ..These studies demonstrate the feasibility of producing transgenic biosensor mice for investigations of signaling processes in intact systems...
  12. ncbi request reprint Delphinidin-3-rutinoside relaxes the bovine ciliary smooth muscle through activation of ETB receptor and NO/cGMP pathway
    Hitoshi Matsumoto
    Food and Health Laboratories, Meiji Seika Ltd, 5 3 1, Chiyoda, Saitama 350 0289, Japan
    Exp Eye Res 80:313-22. 2005
    ....
  13. ncbi request reprint ER stress disrupts Ca2+-signaling complexes and Ca2+ regulation in secretory and muscle cells from PERK-knockout mice
    Guojin Huang
    Department of Physiology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390, USA
    J Cell Sci 119:153-61. 2006
    ....

Research Grants24

  1. Roles of Myosin Light Chain Kinases in the Heart
    James Stull; Fiscal Year: 2007
    ..Results from these studies will provide novel biochemical insights into the physiological regulation and functional importance of cardiac myosin light chain phosphorylation. ..
  2. MYOSIN LIGHT CHAIN KINASE FUNCTION IN SMOOTH MUSCLE
    James Stull; Fiscal Year: 2009
    ..We consider the possibility that nonmuscle myosin may contribute to the stability of membrane adhesion sites necessary for cellular force transmission by intracellular contractile domains in bladder myocytes. ..
  3. Roles of Myosin Light Chain Kinases in the Heart
    James T Stull; Fiscal Year: 2010
    ..Results from these studies will provide novel biochemical insights into the physiological regulation and functional importance of cardiac myosin light chain phosphorylation. ..
  4. MYOSIN LIGHT CHAIN KINASE FUNCTION IN SMOOTH MUSCLE
    James Stull; Fiscal Year: 2003
    ..abstract_text> ..
  5. Signaling Mechanisms in Salivary Gland Cells
    James T Stull; Fiscal Year: 2010
    ..The proposed work explores novel regulatory mechanisms in Ca2+ signaling and their relevance to regulation of SG fluid and electrolyte secretion. ..