Stefan Strack

Summary

Affiliation: University of Iowa
Country: USA

Publications

  1. ncbi request reprint Unfolding-resistant translocase targeting: a novel mechanism for outer mitochondrial membrane localization exemplified by the Bbeta2 regulatory subunit of protein phosphatase 2A
    Ruben K Dagda
    Department of Pharmacology, University of Iowa Carver College of Medicine, Iowa City, Iowa 52242, USA
    J Biol Chem 280:27375-82. 2005
  2. ncbi request reprint Structure of protein phosphatase 2A core enzyme bound to tumor-inducing toxins
    Yongna Xing
    Department of Molecular Biology, Lewis Thomas Laboratory, Princeton University, NJ 08544, USA
    Cell 127:341-53. 2006
  3. pmc Mechanism of neuroprotective mitochondrial remodeling by PKA/AKAP1
    Ronald A Merrill
    Department of Pharmacology, University of Iowa, Iowa City, Iowa, United States of America
    PLoS Biol 9:e1000612. 2011
  4. pmc Cyclin-dependent kinases regulate splice-specific targeting of dynamin-related protein 1 to microtubules
    Stefan Strack
    Department of Pharmacology, University of Iowa, Iowa City, IA 52246, USA
    J Cell Biol 201:1037-51. 2013
  5. ncbi request reprint Protein phosphatase 2A holoenzyme assembly: identification of contacts between B-family regulatory and scaffolding A subunits
    Stefan Strack
    Department of Pharmacology, University of Iowa, Iowa City, Iowa 52242, USA
    J Biol Chem 277:20750-5. 2002
  6. ncbi request reprint Overexpression of the protein phosphatase 2A regulatory subunit Bgamma promotes neuronal differentiation by activating the MAP kinase (MAPK) cascade
    Stefan Strack
    Department of Pharmacology, University of Iowa, College of Medicine, Iowa City 52242, USA
    J Biol Chem 277:41525-32. 2002
  7. ncbi request reprint Critical role for protein phosphatase 2A heterotrimers in mammalian cell survival
    Stefan Strack
    Department of Pharmacology, University of Iowa Carver College of Medicine, Iowa City, Iowa 52242, USA
    J Biol Chem 279:47732-9. 2004
  8. pmc N-terminal phosphorylation of protein phosphatase 2A/Bβ2 regulates translocation to mitochondria, dynamin-related protein 1 dephosphorylation, and neuronal survival
    Ronald A Merrill
    Department of Pharmacology, University of Iowa, Iowa City, IA 52242, USA
    FEBS J 280:662-73. 2013
  9. pmc The protein phosphatase 2A regulatory subunits B'beta and B'delta mediate sustained TrkA neurotrophin receptor autophosphorylation and neuronal differentiation
    Michael J Van Kanegan
    Department of Pharmacology, University of Iowa Carver College of Medicine, Iowa City, Iowa 52242, USA
    Mol Cell Biol 29:662-74. 2009
  10. pmc The spinocerebellar ataxia 12 gene product and protein phosphatase 2A regulatory subunit Bbeta2 antagonizes neuronal survival by promoting mitochondrial fission
    Ruben K Dagda
    Department of Pharmacology, University of Iowa, Iowa City, Iowa 52242, USA
    J Biol Chem 283:36241-8. 2008

Collaborators

Detail Information

Publications32

  1. ncbi request reprint Unfolding-resistant translocase targeting: a novel mechanism for outer mitochondrial membrane localization exemplified by the Bbeta2 regulatory subunit of protein phosphatase 2A
    Ruben K Dagda
    Department of Pharmacology, University of Iowa Carver College of Medicine, Iowa City, Iowa 52242, USA
    J Biol Chem 280:27375-82. 2005
    ..This unfolding-resistant targeting to the mitochondrial translocase is necessary but not sufficient for the proapoptotic activity of Bbeta2, which also requires association with the rest of the PP2A holoenzyme...
  2. ncbi request reprint Structure of protein phosphatase 2A core enzyme bound to tumor-inducing toxins
    Yongna Xing
    Department of Molecular Biology, Lewis Thomas Laboratory, Princeton University, NJ 08544, USA
    Cell 127:341-53. 2006
    ..These structures, together with biochemical analyses, reveal significant insights into PP2A function and serve as a framework for deciphering the diverse roles of PP2A in cellular physiology...
  3. pmc Mechanism of neuroprotective mitochondrial remodeling by PKA/AKAP1
    Ronald A Merrill
    Department of Pharmacology, University of Iowa, Iowa City, Iowa, United States of America
    PLoS Biol 9:e1000612. 2011
    ..Unopposed fusion then promotes formation of a mitochondrial reticulum, which protects neurons from diverse insults...
  4. pmc Cyclin-dependent kinases regulate splice-specific targeting of dynamin-related protein 1 to microtubules
    Stefan Strack
    Department of Pharmacology, University of Iowa, Iowa City, IA 52246, USA
    J Cell Biol 201:1037-51. 2013
    ..Thus, alternative splicing generates a latent, cytoskeletal pool of Drp1 that is selectively mobilized by cyclin-dependent kinase signaling. ..
  5. ncbi request reprint Protein phosphatase 2A holoenzyme assembly: identification of contacts between B-family regulatory and scaffolding A subunits
    Stefan Strack
    Department of Pharmacology, University of Iowa, Iowa City, Iowa 52242, USA
    J Biol Chem 277:20750-5. 2002
    ..By expressing complementary charge-reversal mutants in neuronal PC6-3 cells, we further show that holoenzyme incorporation protects Bgamma from rapid degradation by the ubiquitin/proteasome pathway...
  6. ncbi request reprint Overexpression of the protein phosphatase 2A regulatory subunit Bgamma promotes neuronal differentiation by activating the MAP kinase (MAPK) cascade
    Stefan Strack
    Department of Pharmacology, University of Iowa, College of Medicine, Iowa City 52242, USA
    J Biol Chem 277:41525-32. 2002
    ..Mutational analyses demonstrate that the divergent N terminus is critical for Bgamma activity. These studies implicate PP2A/Bgamma as a positive regulator of MAP kinase signaling in neurons...
  7. ncbi request reprint Critical role for protein phosphatase 2A heterotrimers in mammalian cell survival
    Stefan Strack
    Department of Pharmacology, University of Iowa Carver College of Medicine, Iowa City, Iowa 52242, USA
    J Biol Chem 279:47732-9. 2004
    ..Thus, heterotrimeric PP2A holoenzymes containing the Aalpha subunit and members of all three regulatory subunit families are necessary for mammalian cell viability...
  8. pmc N-terminal phosphorylation of protein phosphatase 2A/Bβ2 regulates translocation to mitochondria, dynamin-related protein 1 dephosphorylation, and neuronal survival
    Ronald A Merrill
    Department of Pharmacology, University of Iowa, Iowa City, IA 52242, USA
    FEBS J 280:662-73. 2013
    ..These results reveal another layer of complexity in the regulation of the mitochondrial fission-fusion equilibrium and its physiological and pathophysiological consequences in the nervous system...
  9. pmc The protein phosphatase 2A regulatory subunits B'beta and B'delta mediate sustained TrkA neurotrophin receptor autophosphorylation and neuronal differentiation
    Michael J Van Kanegan
    Department of Pharmacology, University of Iowa Carver College of Medicine, Iowa City, Iowa 52242, USA
    Mol Cell Biol 29:662-74. 2009
    ....
  10. pmc The spinocerebellar ataxia 12 gene product and protein phosphatase 2A regulatory subunit Bbeta2 antagonizes neuronal survival by promoting mitochondrial fission
    Ruben K Dagda
    Department of Pharmacology, University of Iowa, Iowa City, Iowa 52242, USA
    J Biol Chem 283:36241-8. 2008
    ....
  11. pmc A calcineurin docking motif (LXVP) in dynamin-related protein 1 contributes to mitochondrial fragmentation and ischemic neuronal injury
    Andrew M Slupe
    Department of Pharmacology, University of Iowa, Iowa City, Iowa 52242, USA
    J Biol Chem 288:12353-65. 2013
    ..These results point to the CaN-Drp1 complex as a potential target for neuroprotective therapy of ischemic stroke...
  12. pmc Distinct activation properties of the nuclear factor of activated T-cells (NFAT) isoforms NFATc3 and NFATc4 in neurons
    JASON D ULRICH
    Department of Pharmacology, University of Iowa Carver College of Medicine, Iowa City, Iowa 52242, USA
    J Biol Chem 287:37594-609. 2012
    ....
  13. ncbi request reprint Positive regulation of Raf1-MEK1/2-ERK1/2 signaling by protein serine/threonine phosphatase 2A holoenzymes
    Deanna G Adams
    Department of Pharmacology, Vanderbilt University Medical Center, Nashville, Tennessee 37232, USA
    J Biol Chem 280:42644-54. 2005
    ..Together these findings indicate that PP2A ABalphaC and ABdeltaC holoenzymes function as positive regulators of Raf1-MEK1/2-ERK1/2 signaling by targeting Raf1...
  14. pmc Protein kinase A anchoring via AKAP150 is essential for TRPV1 modulation by forskolin and prostaglandin E2 in mouse sensory neurons
    Katrin Schnizler
    Department of Pharmacology, University of Iowa Carver College of Medicine, Iowa City, Iowa 52242, USA
    J Neurosci 28:4904-17. 2008
    ..Together, these data suggest that PKA anchoring by AKAP150 is essential for the enhancement of TRPV1 function by activation of the PGE(2)/PKA signaling pathway...
  15. pmc Molecular determinants for PP2A substrate specificity: charged residues mediate dephosphorylation of tyrosine hydroxylase by the PP2A/B' regulatory subunit
    Amit Saraf
    Department of Pharmacology, University of Iowa Carver College of Medicine, Iowa City, Iowa 52242, USA
    Biochemistry 49:986-95. 2010
    ....
  16. ncbi request reprint Binding of protein phosphatase 2A to the L-type calcium channel Cav1.2 next to Ser1928, its main PKA site, is critical for Ser1928 dephosphorylation
    Duane D Hall
    Department of Pharmacology, Roy J and Lucille A Carver College of Medicine, University of Iowa, Iowa City, Iowa 52242 1109, USA
    Biochemistry 45:3448-59. 2006
    ..2 is required for effective reversal of PKA-mediated channel phosphorylation. Accordingly, PKA as well as PP2A are constitutively associated with Ca(v)1.2 for its proper regulation by phosphorylation and dephosphorylation of serine 1928...
  17. pmc Allosteric modulation of Drp1 mechanoenzyme assembly and mitochondrial fission by the variable domain
    Stefan Strack
    Department of Pharmacology, The University of Iowa, Iowa City, Iowa 52242, USA
    J Biol Chem 287:10990-1001. 2012
    ..This in turn modulates Arg-376-dependent OMM targeting of Drp1 via multivalent interactions with Mff...
  18. pmc PKA/AKAP1 and PP2A/Bβ2 regulate neuronal morphogenesis via Drp1 phosphorylation and mitochondrial bioenergetics
    Audrey S Dickey
    Department of Pharmacology and Neuroscience Graduate Program, University of Iowa, Iowa City, Iowa 52242, USA
    J Neurosci 31:15716-26. 2011
    ..We propose that the bioenergetic state of mitochondria, rather than their localization or shape per se, is the key effector of Drp1, altering calcium homeostasis to modulate neuronal morphology and connectivity...
  19. pmc Reversible phosphorylation of Drp1 by cyclic AMP-dependent protein kinase and calcineurin regulates mitochondrial fission and cell death
    J Thomas Cribbs
    Department of Pharmacology, University of Iowa Carver College of Medicine, Iowa City, Iowa 52242, USA
    EMBO Rep 8:939-44. 2007
    ..Thus, Drp1 phosphorylation at Ser 656 provides a mechanism for the integration of cAMP and calcium signals in the control of mitochondrial shape, apoptosis and other aspects of mitochondrial function...
  20. doi request reprint Functional characterization of phosphorylation sites in dynamin-related protein 1
    J Thomas Cribbs
    Department of Pharmacology, University of Iowa Carver College of Medicine, Iowa City, Iowa, USA
    Methods Enzymol 457:231-53. 2009
    ..We also discuss primary astrocyte cultures as a model for regulation of cell death and mitochondrial morphology by Drp1 and present ImageJ macro source code for unbiased quantification of mitochondrial shape changes...
  21. ncbi request reprint Differential expression of the B'beta regulatory subunit of protein phosphatase 2A modulates tyrosine hydroxylase phosphorylation and catecholamine synthesis
    Amit Saraf
    Department of Pharmacology, University of Iowa Carver College of Medicine, Iowa City, Iowa 52242, USA
    J Biol Chem 282:573-80. 2007
    ..In summary, we show that B'beta recruits PP2A to modulate TH activity in a tissue- and cell compartment specific fashion...
  22. ncbi request reprint Distinct protein phosphatase 2A heterotrimers modulate growth factor signaling to extracellular signal-regulated kinases and Akt
    Michael J Van Kanegan
    Department of Pharmacology, University of Iowa Carver College of Medicine, Iowa City, Iowa 52242, USA
    J Biol Chem 280:36029-36. 2005
    ..Thus, receptor tyrosine kinase signaling cascades leading to Akt and ERK activation are modulated by PP2A holoenzymes with distinct regulatory properties...
  23. ncbi request reprint A developmentally regulated, neuron-specific splice variant of the variable subunit Bbeta targets protein phosphatase 2A to mitochondria and modulates apoptosis
    Ruben K Dagda
    Department of Pharmacology, University of Iowa Carver College of Medicine, Iowa City 52242, USA
    J Biol Chem 278:24976-85. 2003
    ..Thus, alternative splicing of a mitochondrial localization signal generates a PP2A holoenzyme involved in neuronal survival signaling...
  24. pmc A kinase anchor protein 150 (AKAP150)-associated protein kinase A limits dendritic spine density
    Yuan Lu
    Department of Pharmacology, Roy J and Lucille A Carver College of Medicine, University of Iowa, Iowa City, Iowa 52242, USA
    J Biol Chem 286:26496-506. 2011
    ..However, synaptic transmission and spine density was normal at 8 weeks in KO and D36 mice. Thus AKAP150-independent mechanisms correct the aberrantly high number of active spines in juvenile AKAP150 KO and D36 mice during development...
  25. pmc Selective proteasomal degradation of the B'β subunit of protein phosphatase 2A by the E3 ubiquitin ligase adaptor Kelch-like 15
    Elizabeth A Oberg
    Department of Pharmacology, University of Iowa Carver College of Medicine, Iowa City, Iowa 52242, USA
    J Biol Chem 287:43378-89. 2012
    ..E3 ligase adaptor-mediated control of PP2A holoenzyme composition thereby adds another layer of regulation to cellular dephosphorylation events...
  26. ncbi request reprint Activity-driven postsynaptic translocation of CaMKII
    Michelle A Merrill
    Department of Pharmacology, University of Iowa, Iowa City, IA 52242 1109, USA
    Trends Pharmacol Sci 26:645-53. 2005
    ..In this article, we provide an overview of recent progress in postsynaptic CaMKII anchoring and discuss its implication in synaptic plasticity and the etiology and potential treatments of neurological diseases...
  27. pmc Cell signaling and mitochondrial dynamics: Implications for neuronal function and neurodegenerative disease
    Theodore J Wilson
    Department of Molecular and Cellular Biology Program, University of Iowa, Carver College of Medicine, Iowa City 52242, IA, USA
    Neurobiol Dis 51:13-26. 2013
    ..We also review recent evidence that mitochondrial dynamics has profound consequences for neuronal development and synaptic transmission and discuss implications for clinical translation...
  28. pmc CaMKII determines mitochondrial stress responses in heart
    Mei ling A Joiner
    Department of Internal Medicine and Cardiovascular Center, Carver College of Medicine, University of Iowa, Iowa City, Iowa 52242, USA
    Nature 491:269-73. 2012
    ....
  29. pmc Mitochondria: A kinase anchoring protein 1, a signaling platform for mitochondrial form and function
    Ronald A Merrill
    Department of Pharmacology, University of Iowa, Iowa City, USA
    Int J Biochem Cell Biol 48:92-6. 2014
    ..Recent evidence suggests that AKAP1 has critical roles in neuronal development and survival, which are mediated by inhibitory phosphorylation of Drp1 and maintenance of mitochondrial integrity. ..
  30. pmc Products of oxidative stress inhibit aldehyde oxidation and reduction pathways in dopamine catabolism yielding elevated levels of a reactive intermediate
    Yunden Jinsmaa
    Division of Medicinal and Natural Products Chemistry, College of Pharmacy, and Department of Pharmacology, College of Medicine, The University of Iowa, 115 South Grand Avenue, Iowa City, Iowa 52242, USA
    Chem Res Toxicol 22:835-41. 2009
    ....
  31. ncbi request reprint Structure of the protein phosphatase 2A holoenzyme
    Yanhui Xu
    Department of Molecular Biology, Lewis Thomas Laboratory, Princeton University, Princeton, NJ 08544, USA
    Cell 127:1239-51. 2006
    ..This structure reveals significant ramifications for understanding the function and regulation of PP2A...

Research Grants18

  1. Kinase/Phosphatase-mediated Mitochondrial Restructuring in Neuroprotection
    Stefan Strack; Fiscal Year: 2009
    ..Finally, Aim 4 characterizes Bp2 null mice for mitochondrial changes and resistance to ischemic injury. ..
  2. Regulatory subunits of PP2A in neuronal function
    Stefan Strack; Fiscal Year: 2009
    ..These studies are significant in that they advance our understanding of the critical regulatory enzyme PP2A with the ultimate goal of better therapies for several neurodegenerative disorders. ..
  3. Kinase/Phosphatase-mediated Mitochondrial Restructuring in Neuroprotection
    Stefan Strack; Fiscal Year: 2010
    ..Finally, Aim 4 characterizes Bp2 null mice for mitochondrial changes and resistance to ischemic injury. ..
  4. Regulation of Mitochondrial Fission/Fusion by PP2A and PKA in Neurons
    Stefan Strack; Fiscal Year: 2007
    ..Our studies may ultimately lead to better therapies for stroke and neurodegenerative disorders. ..
  5. Regulatory subunits of PP2A in neuronal function
    Stefan Strack; Fiscal Year: 2007
    ..These studies will significantly advance our understanding of how the ubiquitous PP2A enzyme regulates specifically neuronal functions, and may lead to the development of drugs that target neuronal PP2A isoforms. ..
  6. Regulatory subunits of PP2A in neuronal function
    Stefan Strack; Fiscal Year: 2006
    ..These studies will significantly advance our understanding of how the ubiquitous PP2A enzyme regulates specifically neuronal functions, and may lead to the development of drugs that target neuronal PP2A isoforms. ..
  7. Regulatory subunits of PP2A in neuronal function
    Stefan Strack; Fiscal Year: 2010
    ..These studies are significant in that they advance our understanding of the critical regulatory enzyme PP2A with the ultimate goal of better therapies for several neurodegenerative disorders. ..
  8. Regulation of Mitochondrial Fission/Fusion by PP2A and PKA in Neurons
    Stefan Strack; Fiscal Year: 2009
    ..Our studies may ultimately lead to better therapies for stroke and neurodegenerative disorders. ..
  9. Regulation of Mitochondrial Fission/Fusion by PP2A and PKA in Neurons
    Stefan Strack; Fiscal Year: 2009
    ..Our studies may ultimately lead to better therapies for stroke and neurodegenerative disorders. ..
  10. Regulatory subunits of PP2A in neuronal function
    Stefan Strack; Fiscal Year: 2003
    ..These studies will significantly advance our understanding of how the ubiquitous PP2A enzyme regulates specifically neuronal functions, and may lead to the development of drugs that target neuronal PP2A isoforms. ..
  11. Kinase/Phosphatase-mediated Mitochondrial Restructuring in Neuroprotection
    Stefan Strack; Fiscal Year: 2007
    ..Finally, Aim 4 characterizes Bp2 null mice for mitochondrial changes and resistance to ischemic injury. ..
  12. Regulatory subunits of PP2A in neuronal function
    Stefan Strack; Fiscal Year: 2011
    ..These studies are significant in that they advance our understanding of the critical regulatory enzyme PP2A with the ultimate goal of better therapies for several neurodegenerative disorders. ..