Andrew F Stewart

Summary

Affiliation: University of Pittsburgh
Country: USA

Publications

  1. doi request reprint The United States endocrinology workforce: a supply-demand mismatch
    Andrew F Stewart
    Division of Endocrinology, University of Pittsburgh School of Medicine, 200 Lothrop Street, Pittsburgh, Pennsylvania 15213, USA
    J Clin Endocrinol Metab 93:1164-6. 2008
  2. ncbi request reprint Clinical practice. Hypercalcemia associated with cancer
    Andrew F Stewart
    Division of Endocrinology, University of Pittsburgh School of Medicine, Pittsburgh 15213, USA
    N Engl J Med 352:373-9. 2005
  3. pmc Survey of the human pancreatic beta-cell G1/S proteome reveals a potential therapeutic role for cdk-6 and cyclin D1 in enhancing human beta-cell replication and function in vivo
    Nathalie Fiaschi-Taesch
    Division of Endocrinology, The University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
    Diabetes 58:882-93. 2009
  4. pmc A 7-day continuous infusion of PTH or PTHrP suppresses bone formation and uncouples bone turnover
    Mara J Horwitz
    Division of Endocrinology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA
    J Bone Miner Res 26:2287-97. 2011
  5. pmc Cytoplasmic-nuclear trafficking of G1/S cell cycle molecules and adult human β-cell replication: a revised model of human β-cell G1/S control
    Nathalie M Fiaschi-Taesch
    Division of Endocrinology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
    Diabetes 62:2460-70. 2013
  6. pmc Lessons from the first comprehensive molecular characterization of cell cycle control in rodent insulinoma cell lines
    Irene Cozar-Castellano
    Department of Medicine, Division of Endocrinology and Metabolism, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
    Diabetes 57:3056-68. 2008
  7. pmc Parathyroid hormone-related protein enhances human ß-cell proliferation and function with associated induction of cyclin-dependent kinase 2 and cyclin E expression
    Nagesha Guthalu Kondegowda
    Division of Endocrinology, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
    Diabetes 59:3131-8. 2010
  8. pmc Hepatocyte growth factor enhances engraftment and function of nonhuman primate islets
    Nathalie M Fiaschi-Taesch
    Division of Endocrinology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
    Diabetes 57:2745-54. 2008
  9. pmc Induction of human beta-cell proliferation and engraftment using a single G1/S regulatory molecule, cdk6
    Nathalie M Fiaschi-Taesch
    Division of Endocrinology, The University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
    Diabetes 59:1926-36. 2010
  10. ncbi request reprint Mutant parathyroid hormone-related protein, devoid of the nuclear localization signal, markedly inhibits arterial smooth muscle cell cycle and neointima formation by coordinate up-regulation of p15Ink4b and p27kip1
    Nathalie Fiaschi-Taesch
    Division of Endocrinology and Metabolism, University of Pittsburgh School of Medicine, Pennsylvania 15213, USA
    Endocrinology 150:1429-39. 2009

Research Grants

  1. Anabolic Skeletal Agents for Osteoporosis
    Andrew Stewart; Fiscal Year: 2004
  2. Islet Allograft Gene Therapy for Primate Diabetes
    Andrew Stewart; Fiscal Year: 2005
  3. RESEARCH TRAINING IN DIABETES AND ENDOCRINOLOGY
    Andrew Stewart; Fiscal Year: 2007

Collaborators

Detail Information

Publications43

  1. doi request reprint The United States endocrinology workforce: a supply-demand mismatch
    Andrew F Stewart
    Division of Endocrinology, University of Pittsburgh School of Medicine, 200 Lothrop Street, Pittsburgh, Pennsylvania 15213, USA
    J Clin Endocrinol Metab 93:1164-6. 2008
  2. ncbi request reprint Clinical practice. Hypercalcemia associated with cancer
    Andrew F Stewart
    Division of Endocrinology, University of Pittsburgh School of Medicine, Pittsburgh 15213, USA
    N Engl J Med 352:373-9. 2005
  3. pmc Survey of the human pancreatic beta-cell G1/S proteome reveals a potential therapeutic role for cdk-6 and cyclin D1 in enhancing human beta-cell replication and function in vivo
    Nathalie Fiaschi-Taesch
    Division of Endocrinology, The University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
    Diabetes 58:882-93. 2009
    ....
  4. pmc A 7-day continuous infusion of PTH or PTHrP suppresses bone formation and uncouples bone turnover
    Mara J Horwitz
    Division of Endocrinology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA
    J Bone Miner Res 26:2287-97. 2011
    ..The findings also highlight similarities and differences between the model and HPT...
  5. pmc Cytoplasmic-nuclear trafficking of G1/S cell cycle molecules and adult human β-cell replication: a revised model of human β-cell G1/S control
    Nathalie M Fiaschi-Taesch
    Division of Endocrinology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
    Diabetes 62:2460-70. 2013
    ..In addition to known obstacles to β-cell proliferation, cytoplasmic-to-nuclear trafficking of G1/S molecules may represent an obstacle as well as a therapeutic opportunity for human β-cell expansion. ..
  6. pmc Lessons from the first comprehensive molecular characterization of cell cycle control in rodent insulinoma cell lines
    Irene Cozar-Castellano
    Department of Medicine, Division of Endocrinology and Metabolism, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
    Diabetes 57:3056-68. 2008
    ..Rodent insulinoma cell lines may serve as a model for designing continuously replicating human beta-cell lines and provide clues as to the central cell cycle regulatory molecules in the beta-cell...
  7. pmc Parathyroid hormone-related protein enhances human ß-cell proliferation and function with associated induction of cyclin-dependent kinase 2 and cyclin E expression
    Nagesha Guthalu Kondegowda
    Division of Endocrinology, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
    Diabetes 59:3131-8. 2010
    ..Based on this, we hypothesized that PTH1R is expressed in human β-cells and that PTHrP has the potential to enhance human β-cell proliferation and/or function...
  8. pmc Hepatocyte growth factor enhances engraftment and function of nonhuman primate islets
    Nathalie M Fiaschi-Taesch
    Division of Endocrinology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
    Diabetes 57:2745-54. 2008
    ..Here, we asked whether these prior observations in rodent models extend to nonhuman primate (NHP) islets...
  9. pmc Induction of human beta-cell proliferation and engraftment using a single G1/S regulatory molecule, cdk6
    Nathalie M Fiaschi-Taesch
    Division of Endocrinology, The University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
    Diabetes 59:1926-36. 2010
    ..We explored the presence, subcellular localization, and function of five early G1/S phase molecules-cyclins D1-3 and cdk 4 and 6-in the adult human beta-cell...
  10. ncbi request reprint Mutant parathyroid hormone-related protein, devoid of the nuclear localization signal, markedly inhibits arterial smooth muscle cell cycle and neointima formation by coordinate up-regulation of p15Ink4b and p27kip1
    Nathalie Fiaschi-Taesch
    Division of Endocrinology and Metabolism, University of Pittsburgh School of Medicine, Pennsylvania 15213, USA
    Endocrinology 150:1429-39. 2009
    ..These studies provide a mechanistic understanding of DeltaNLS-PTH-related protein actions, and suggest that DeltaNLS-PTH-related protein may have particular efficacy for the prevention of arterial restenosis...
  11. pmc The retinoblastoma protein and its homolog p130 regulate the G1/S transition in pancreatic beta-cells
    George Harb
    Division of Endocrinology and Metabolism, Department of Medicine, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
    Diabetes 58:1852-62. 2009
    ..Therefore, we explored the effects of individual loss of either p130 or p107 in addition to the simultaneous loss of both pRb/p130 on the beta-cell...
  12. ncbi request reprint Parathyroid-hormone-related protein as a regulator of pRb and the cell cycle in arterial smooth muscle
    Nathalie Fiaschi-Taesch
    Division of Endocrinology and Metabolism, BST E 1140, Endocrinology, University of Pittsburgh School of Medicine, 3550 Terrace St, Pittsburgh, PA 15213, USA
    Circulation 110:177-85. 2004
    ....
  13. pmc Lactation and bone turnover: a conundrum of marked bone loss in the setting of coupled bone turnover
    Raquel M Carneiro
    Division of Endocrinology, University of Pittsburgh School of Medicine, NW 810, UPMC Montefiore, 3459 5th Avenue, Pittsburgh, Pennsylvania 15213, USA
    J Clin Endocrinol Metab 95:1767-76. 2010
    ..Uncoupling of bone turnover, which is seen in other prototypical states of bone loss, would seem the likely explanation. However, the most current markers of bone turnover have not been studied in human lactation...
  14. ncbi request reprint Continuous PTH and PTHrP infusion causes suppression of bone formation and discordant effects on 1,25(OH)2 vitamin D
    Mara J Horwitz
    Division of Endocrinology, The University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA
    J Bone Miner Res 20:1792-803. 2005
    ..Continuous infusion of both PTH and PTHrP results in marked and prolonged suppression of bone formation; renal 1,25(OH)2D synthesis was stimulated effectively by PTH but poorly by PTHrP...
  15. pmc Parathyroid hormone-related protein for the treatment of postmenopausal osteoporosis: defining the maximal tolerable dose
    Mara J Horwitz
    Division of Endocrinology, Falk 560, University of Pittsburgh School of Medicine, 3601 Fifth Avenue, Pittsburgh Pennsylvania 15213, USA
    J Clin Endocrinol Metab 95:1279-87. 2010
    ..Surprisingly, PTHrP in the high doses studied activates 1,25 dihydroxyvitamin D production. Dosing information obtained herein can be used to design a longer term head-to-head comparative efficacy trial of PTHrP vs. PTH...
  16. pmc Human pancreatic β-cell G1/S molecule cell cycle atlas
    Nathalie M Fiaschi-Taesch
    Division of Endocrinology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
    Diabetes 62:2450-9. 2013
    ..Thus, in addition to known obstacles to human β-cell proliferation, restriction of G1/S molecules to the cytoplasm of the human β-cell represents an unanticipated obstacle to therapeutic human β-cell expansion. ..
  17. ncbi request reprint Safety and tolerability of subcutaneous PTHrP(1-36) in healthy human volunteers: a dose escalation study
    Mara J Horwitz
    Division of Endocrinology, University of Pittsburgh School of Medicine, Falk 580 3601 Fifth Ave, Pittsburgh, PA 15213, USA
    Osteoporos Int 17:225-30. 2006
    ..0 mg. These findings indicate that the therapeutic window for PTHrP(1-36) in humans is wide and permit the design and implementation of longer safety and efficacy trials...
  18. ncbi request reprint Cellular mechanism through which parathyroid hormone-related protein induces proliferation in arterial smooth muscle cells: definition of an arterial smooth muscle PTHrP/p27kip1 pathway
    Nathalie Fiaschi-Taesch
    Division of Endocrinology, University of Pittsburgh School of Medicine, PA 15213, USA
    Circ Res 99:933-42. 2006
    ....
  19. ncbi request reprint Tissue-specific deletion of the retinoblastoma protein in the pancreatic beta-cell has limited effects on beta-cell replication, mass, and function
    Rupangi C Vasavada
    Division of Endocrinology, University of Pittsburgh School of Medicine, 200 Lothrop St, Pittsburgh, PA 15213, USA
    Diabetes 56:57-64. 2007
    ..This may reflect fundamental inaccuracies in models of beta-cell cycle control or complementation for pRb by undefined proteins...
  20. ncbi request reprint Molecular control of cell cycle progression in the pancreatic beta-cell
    Irene Cozar-Castellano
    Division of Endocrinology and Metabolism, BST E 1140, The University of Pittsburgh School of Medicine, 200 Lothrop Street, Pennsylvania 15213, USA
    Endocr Rev 27:356-70. 2006
    ....
  21. pmc ChREBP mediates glucose-stimulated pancreatic β-cell proliferation
    Mallikarjuna R Metukuri
    Department of Medicine, Division of Endocrinology and Metabolism, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
    Diabetes 61:2004-15. 2012
    ..Overexpression of ChREBP amplified glucose-stimulated proliferation in rat and human β-cells, with concomitant increases in cyclin gene expression. In conclusion, ChREBP mediates glucose-stimulated proliferation in pancreatic β-cells...
  22. pmc cMyc is a principal upstream driver of beta-cell proliferation in rat insulinoma cell lines and is an effective mediator of human beta-cell replication
    Esra Karslioglu
    Division of Endocrinology, The University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15260, USA
    Mol Endocrinol 25:1760-72. 2011
    ..The cMyc pathway provides potential approaches, targets, and tools for driving and sustaining human β-cell replication...
  23. ncbi request reprint Induction of beta-cell proliferation and retinoblastoma protein phosphorylation in rat and human islets using adenovirus-mediated transfer of cyclin-dependent kinase-4 and cyclin D1
    Irene Cozar-Castellano
    Division of Endocrinology and Metabolism, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA
    Diabetes 53:149-59. 2004
    ..This approach will allow detailed molecular study of the cellular mechanisms regulating the cell cycle in beta-cells, beta-cell lines, and stem cell-derived beta-cells...
  24. pmc Regulated and reversible induction of adult human β-cell replication
    Karen K Takane
    University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
    Diabetes 61:418-24. 2012
    ..Human β-cells can be induced to proliferate or arrest in a regulated, reversible manner, temporally and quantitatively mimicking the transient perinatal physiological proliferation that occurs in human β-cells...
  25. ncbi request reprint Improving islet transplantation by gene delivery of hepatocyte growth factor (HGF) and its downstream target, protein kinase B (PKB)/Akt
    Nathalie Fiaschi-Taesch
    Division of Endocrinology, University of Pittsburgh, Pittsburgh, PA, USA
    Cell Biochem Biophys 48:191-9. 2007
    ....
  26. ncbi request reprint The cell cycle inhibitory protein p21cip is not essential for maintaining beta-cell cycle arrest or beta-cell function in vivo
    Irene Cozar-Castellano
    Division of Endocrinology, BST E 1140, University of Pittsburgh School of Medicine, 200 Lothrop St, Pittsburgh, PA 15213, USA
    Diabetes 55:3271-8. 2006
    ..These studies underscore marked inhibitory pressure and the complexity and plasticity of inhibitory pathways that restrain beta-cell replication...
  27. pmc Disruption of hepatocyte growth factor/c-Met signaling enhances pancreatic beta-cell death and accelerates the onset of diabetes
    Jose Mellado-Gil
    Department of Medicine, Division of Endocrinology and Metabolism, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
    Diabetes 60:525-36. 2011
    ..To determine the role of hepatocyte growth factor (HGF)/c-Met on β-cell survival in diabetogenic conditions in vivo and in response to cytokines in vitro...
  28. pmc A comparison of parathyroid hormone-related protein (1-36) and parathyroid hormone (1-34) on markers of bone turnover and bone density in postmenopausal women: the PrOP study
    Mara J Horwitz
    Division of Endocrinology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA
    J Bone Miner Res 28:2266-76. 2013
    ..Longer-term studies using lower doses of PTHrP(1-36) are needed to define both the optimal dose and full clinical benefits of PTHrP. © 2013 American Society for Bone and Mineral Research...
  29. pmc Molecular engineering human hepatocytes into pancreatic beta cells for diabetes therapy
    Irene Cozar-Castellano
    Division of Endocrinology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA
    Proc Natl Acad Sci U S A 102:7781-2. 2005
  30. ncbi request reprint Evaluation of beta-cell replication in mice transgenic for hepatocyte growth factor and placental lactogen: comprehensive characterization of the G1/S regulatory proteins reveals unique involvement of p21cip
    Irene Cozar-Castellano
    Division of Endocrinology, BST E 1140, University of Pittsburgh School of Medicine, 200 Lothrop Street, Pittsburgh, PA 15213, USA
    Diabetes 55:70-7. 2006
    ..Homozygous p21(cip) loss releases islets from growth inhibition, markedly enhancing proliferation in response to HGF and placental lactogen...
  31. ncbi request reprint Translational implications of the parathyroid calcium receptor
    Andrew F Stewart
    Division of Endocrinology and Metabolism, University of Pittsburgh School of Medicine, Pittsburgh, USA
    N Engl J Med 351:324-6. 2004
  32. ncbi request reprint Hepatocyte growth factor gene therapy for pancreatic islets in diabetes: reducing the minimal islet transplant mass required in a glucocorticoid-free rat model of allogeneic portal vein islet transplantation
    Juan Carlos Lopez-Talavera
    Division of Endocrinology, BST E 1140, University of Pittsburgh School of Medicine, 3550 Terrace Street, Pittsburgh, PA 15213, USA
    Endocrinology 145:467-74. 2004
    ..This approach may enhance islet transplantation outcomes in humans...
  33. ncbi request reprint Direct comparison of sustained infusion of human parathyroid hormone-related protein-(1-36) [hPTHrP-(1-36)] versus hPTH-(1-34) on serum calcium, plasma 1,25-dihydroxyvitamin D concentrations, and fractional calcium excretion in healthy human volunteers
    Mara J Horwitz
    Division of Endocrinology, University of Pittsburgh School of Medicine, Pennsylvania 15213, USA
    J Clin Endocrinol Metab 88:1603-9. 2003
    ....
  34. pmc Mammary-specific deletion of parathyroid hormone-related protein preserves bone mass during lactation
    Joshua N VanHouten
    Section of Endocrinology and Metabolism, Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut 06520 8020, USA
    J Clin Invest 112:1429-36. 2003
    ..We conclude that during lactation mammary epithelial cells are a source of circulating PTHrP that promotes bone loss by increasing rates of bone resorption...
  35. ncbi request reprint Hypercalcemia
    Barbara K Kinder
    Department of Surgery, Surgical Oncology, and Endocrinology, Yale University School of Medicine, New Haven, Connecticut, USA
    Curr Probl Surg 39:349-448. 2002
  36. ncbi request reprint Hyperparathyroidism, humoral hypercalcemia of malignancy, and the anabolic actions of parathyroid hormone and parathyroid hormone-related protein on the skeleton
    Andrew F Stewart
    J Bone Miner Res 17:758-62. 2002
    ....
  37. ncbi request reprint Overexpression of parathyroid hormone-related protein inhibits pancreatic beta-cell death in vivo and in vitro
    Ana Cebrian
    Division of Endocrinology, University of Pittsburgh School of Medicine, Pennsylvania 15213, USA
    Diabetes 51:3003-13. 2002
    ..Finally, we demonstrated that NH(2)-terminal PTHrP inhibits beta-cell death. These findings support the concept that PTHrP overexpression increases islet mass in transgenic mice through inhibition of beta-cell death...
  38. ncbi request reprint Adenovirus-mediated hepatocyte growth factor expression in mouse islets improves pancreatic islet transplant performance and reduces beta cell death
    Adolfo Garcia-Ocana
    Division of Endocrinology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA
    J Biol Chem 278:343-51. 2003
    ..In summary, adenoviral delivery of HGF to murine islets ex vivo improves islet transplant survival and blood glucose control in a subcapsular renal graft model in immuno-incompetent diabetic mice...
  39. ncbi request reprint Minireview: parathyroid hormone-related protein as an intracrine factor--trafficking mechanisms and functional consequences
    Nathalie M Fiaschi-Taesch
    Division of Endocrinology and Metabolism, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15217, USA
    Endocrinology 144:407-11. 2003
    ..This review discusses the mechanisms by which PTHrP may gain access to the nucleus/nucleolus and the functional consequences of this nuclear entry by PTHrP...
  40. ncbi request reprint Short-term, high-dose parathyroid hormone-related protein as a skeletal anabolic agent for the treatment of postmenopausal osteoporosis
    Mara J Horwitz
    Division of Endocrinology, The University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA
    J Clin Endocrinol Metab 88:569-75. 2003
    ..Despite the high doses, PTHrP was well tolerated. Larger clinical trials are required to confirm these results and fully assess the anabolic potential of PTHrP in osteoporosis...
  41. ncbi request reprint Parathyroid hormone-related protein: an essential physiological regulator of adult bone mass
    Alessandro Bisello
    Endocrinology 145:3551-3. 2004
  42. ncbi request reprint Type 1 parathyroid hormone receptor expression level modulates renal tone and plasma renin activity in spontaneously hypertensive rat
    Thierry Massfelder
    Section of Renovascular Pharmacology and Physiology INSERM ULP, University Louis Pasteur School of Medicine, Strasbourg, France
    J Am Soc Nephrol 13:639-48. 2002
    ..Overall, PTHrP significantly contributes to the homeostasis of renal and systemic hemodynamics in SHR...
  43. pmc Hypoparathyroidism: is it time for replacement therapy?
    Mara J Horwitz
    J Clin Endocrinol Metab 93:3307-9. 2008

Research Grants5

  1. Anabolic Skeletal Agents for Osteoporosis
    Andrew Stewart; Fiscal Year: 2004
    ..Thus, the Aim of this proposal is to develop a meeting at the NIH Lister Hill center focused on advances in Skeletal Anabolic Agents and the Treatment of Osteoporosis. ..
  2. Islet Allograft Gene Therapy for Primate Diabetes
    Andrew Stewart; Fiscal Year: 2005
    ..We are optimistic that these studies will likely achieve their goal, and will serve in the near term as the third and final preclinical step required for similar studies in humans with diabetes. ..
  3. RESEARCH TRAINING IN DIABETES AND ENDOCRINOLOGY
    Andrew Stewart; Fiscal Year: 2007
    ..abstract_text> ..