Lawrence Stern

Summary

Affiliation: University of Massachusetts Medical School
Country: USA

Publications

  1. pmc Human CD4+ T cell response to human herpesvirus 6
    Maria D Nastke
    Department of Pathology, University of Massachusetts Medical School, Worcester, Massachusetts, USA
    J Virol 86:4776-92. 2012
  2. pmc HLA-DR: molecular insights and vaccine design
    Lawrence J Stern
    Department of Pathology, Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01655, USA
    Curr Pharm Des 15:3249-61. 2009
  3. ncbi request reprint Characterizing MHC-associated peptides by mass spectrometry
    Lawrence J Stern
    Department of Pathology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA
    J Immunol 179:2667-8. 2007
  4. ncbi request reprint Homooligomerization of the cytoplasmic domain of the T cell receptor zeta chain and of other proteins containing the immunoreceptor tyrosine-based activation motif
    Alexander Sigalov
    Department of Pathology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, Massachusetts 01655, USA
    Biochemistry 43:2049-61. 2004
  5. pmc The intrinsically disordered cytoplasmic domain of the T cell receptor zeta chain binds to the nef protein of simian immunodeficiency virus without a disorder-to-order transition
    Alexander B Sigalov
    Department of Pathology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, Massachusetts 01655, USA
    Biochemistry 47:12942-4. 2008
  6. pmc Lipid-binding activity of intrinsically unstructured cytoplasmic domains of multichain immune recognition receptor signaling subunits
    Alexander B Sigalov
    Department of Pathology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, Massachusetts 01655, USA
    Biochemistry 45:15731-9. 2006
  7. pmc Pseudo-merohedral twinning and noncrystallographic symmetry in orthorhombic crystals of SIVmac239 Nef core domain bound to different-length TCRzeta fragments
    Walter M Kim
    University of Massachusetts Medical School, USA
    Acta Crystallogr D Biol Crystallogr 66:163-75. 2010
  8. pmc Fluorogenic probes for monitoring peptide binding to class II MHC proteins in living cells
    Prasanna Venkatraman
    Department of Pathology, University of Massachusetts Medical School, 55 Lake Ave North, Worcester, Massachusetts 01655, USA
    Nat Chem Biol 3:222-8. 2007
  9. pmc Vaccinia peptides eluted from HLA-DR1 isolated from virus-infected cells are recognized by CD4+ T cells from a vaccinated donor
    Iwona Strug
    Department of Pathology, Proteomic and Mass Spectrometry Core Facility, Center for Infectious Disease and Vaccine Research, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    J Proteome Res 7:2703-11. 2008
  10. pmc Human CD4+ T cell epitopes from vaccinia virus induced by vaccination or infection
    J Mauricio Calvo-Calle
    Department of Pathology, University of Massachusetts Medical School, Worcester, Massachusetts, USA
    PLoS Pathog 3:1511-29. 2007

Research Grants

Collaborators

  • Alexander B Sigalov
  • L Santambrogio
  • Michael A Brehm
  • Vladimir N Uversky
  • Masanori Terajima
  • Osman Bilsel
  • Raymond M Welsh
  • JACK STROMINGER
  • Eric J Sundberg
  • Christian S Parry
  • Manuel E Patarroyo
  • F Re
  • J Mauricio Calvo-Calle
  • Sriram Chitta
  • Jennifer D Stone
  • Zarixia Zavala-Ruiz
  • Iwona Strug
  • Maria D Nastke
  • Gregory J Carven
  • Zu T Shen
  • Walter M Kim
  • Sabrina S Vollers
  • Corrie A Painter
  • John Cruz
  • Francis A Ennis
  • Shibani Mitra-Kaushik
  • Prasanna Venkatraman
  • Stephen L De Wall
  • Carlos Parra-Lopez
  • Rick Baggio
  • Ilaria Potolicchio
  • Jaime E Arenas
  • Michelle Palmer
  • Vaclav Horejsi
  • Qing Ge
  • Aniuska Becerra
  • Omar Dominguez-Amorocho
  • Laura Gibson
  • Liusong Yin
  • Liisa K Selin
  • Keith A Daniels
  • Anthony Cruz
  • James E Evans
  • Karin M Green
  • GUSTAVO E LOPEZ
  • Stephen P Baker
  • Tina T Nguyen
  • Maria Dorothea Nastke
  • Barbara Imperiali
  • Matthieu Sainlos
  • Luz Mary Salazar
  • Thomas O Cameron
  • Luis E Vargas
  • Corrie Painter
  • Timothy J Mitchison
  • Elizabeth Nardin
  • Don C Wiley
  • Brian S DeDecker
  • Rajintha Bandaranayake
  • Giovanna Crisi
  • Graca Raposo
  • Dora Fonseca
  • Xiaonan Xu
  • Anthony Chiulli
  • Rick F Baggio
  • Matthew W Anderson
  • Mia M Rushe
  • Jack Gorski
  • Ivan Hilgert
  • Roy A Mariuzza
  • Iat C Chan
  • Daniel B DeOliveira
  • Jennifer Svendsen
  • M Todd Thompson
  • Jennifer R Cochran
  • Jianzhu Chen
  • Herman N Eisen
  • Mia Rushe

Detail Information

Publications28

  1. pmc Human CD4+ T cell response to human herpesvirus 6
    Maria D Nastke
    Department of Pathology, University of Massachusetts Medical School, Worcester, Massachusetts, USA
    J Virol 86:4776-92. 2012
    ..These findings provide a window into the immune response to HHV-6 and provide a basis for tracking HHV-6 cellular immune responses...
  2. pmc HLA-DR: molecular insights and vaccine design
    Lawrence J Stern
    Department of Pathology, Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01655, USA
    Curr Pharm Des 15:3249-61. 2009
    ..Finally, using malaria as a model, we describe the development of a minimal subunit vaccine for the human malaria parasite Plasmodium falciparum...
  3. ncbi request reprint Characterizing MHC-associated peptides by mass spectrometry
    Lawrence J Stern
    Department of Pathology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA
    J Immunol 179:2667-8. 2007
  4. ncbi request reprint Homooligomerization of the cytoplasmic domain of the T cell receptor zeta chain and of other proteins containing the immunoreceptor tyrosine-based activation motif
    Alexander Sigalov
    Department of Pathology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, Massachusetts 01655, USA
    Biochemistry 43:2049-61. 2004
    ..Phosphorylation of zeta(cyt) and FcepsilonRIgamma(cyt) does not significantly alter their oligomerization behavior. The implications of these results for transmembrane signaling and cellular activation by immune receptors are discussed...
  5. pmc The intrinsically disordered cytoplasmic domain of the T cell receptor zeta chain binds to the nef protein of simian immunodeficiency virus without a disorder-to-order transition
    Alexander B Sigalov
    Department of Pathology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, Massachusetts 01655, USA
    Biochemistry 47:12942-4. 2008
    ..Thus, our findings oppose the generally accepted view of the behavior of intrinsically disordered proteins and provide new evidence of the existence of specific interactions for unfolded protein molecules...
  6. pmc Lipid-binding activity of intrinsically unstructured cytoplasmic domains of multichain immune recognition receptor signaling subunits
    Alexander B Sigalov
    Department of Pathology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, Massachusetts 01655, USA
    Biochemistry 45:15731-9. 2006
    ..Phosphorylation of zetacyt and FcepsilonRIgammacyt does not alter their random-coil conformation but weakens binding to membranes. The implications of these results for transmembrane signal transduction by immune receptors are discussed...
  7. pmc Pseudo-merohedral twinning and noncrystallographic symmetry in orthorhombic crystals of SIVmac239 Nef core domain bound to different-length TCRzeta fragments
    Walter M Kim
    University of Massachusetts Medical School, USA
    Acta Crystallogr D Biol Crystallogr 66:163-75. 2010
    ..The combination of NCS rotations that were nearly parallel to the twin operation (k, h, -l) and a and b unit-cell parameters that were nearly identical predisposed the P2(1)2(1)2(1) crystal form to pseudo-merohedral twinning...
  8. pmc Fluorogenic probes for monitoring peptide binding to class II MHC proteins in living cells
    Prasanna Venkatraman
    Department of Pathology, University of Massachusetts Medical School, 55 Lake Ave North, Worcester, Massachusetts 01655, USA
    Nat Chem Biol 3:222-8. 2007
    ..Using these probes we have tracked a developmentally regulated cell-surface peptide-binding activity in primary human monocyte-derived dendritic cells...
  9. pmc Vaccinia peptides eluted from HLA-DR1 isolated from virus-infected cells are recognized by CD4+ T cells from a vaccinated donor
    Iwona Strug
    Department of Pathology, Proteomic and Mass Spectrometry Core Facility, Center for Infectious Disease and Vaccine Research, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    J Proteome Res 7:2703-11. 2008
    ..The peptides, derived from the I6L, D6R, and A10L viral proteins, were 15 residues in length, bound efficiently to HLA-DR1 as synthetic peptides, and were recognized by vaccinia-specific CD4 (+) T cells obtained from an immunized donor...
  10. pmc Human CD4+ T cell epitopes from vaccinia virus induced by vaccination or infection
    J Mauricio Calvo-Calle
    Department of Pathology, University of Massachusetts Medical School, Worcester, Massachusetts, USA
    PLoS Pathog 3:1511-29. 2007
    ..Moreover, the epitope identification approach developed here should find application to other large-genome pathogens...
  11. pmc Class II major histocompatibility complex tetramer staining: progress, problems, and prospects
    Sabrina S Vollers
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA, USA
    Immunology 123:305-13. 2008
    ..In this review we discuss which problems in class II MHC tetramer staining have been solved to date, and which matters remain to be considered...
  12. ncbi request reprint Human cytotoxic CD4+ T cells recognize HLA-DR1-restricted epitopes on vaccinia virus proteins A24R and D1R conserved among poxviruses
    Shibani Mitra-Kaushik
    Center for Infectious Disease and Vaccine Research, University of Massachusetts Medical School, Worcester, MA 01655, USA
    J Immunol 179:1303-12. 2007
    ....
  13. pmc Model for the peptide-free conformation of class II MHC proteins
    Corrie A Painter
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts, United States of America
    PLoS ONE 3:e2403. 2008
    ..Major histocompatibility complex proteins are believed to undergo significant conformational changes concomitant with peptide binding, but structural characterization of these changes has remained elusive...
  14. doi request reprint GMCSF in the absence of other cytokines sustains human dendritic cell precursors with T cell regulatory activity and capacity to differentiate into functional dendritic cells
    Sriram Chitta
    University of Massachusetts Medical School, Department of Pathology, Worcester, MA 10655, United States
    Immunol Lett 116:41-54. 2008
    ..We suggest that these cells may provide a useful model to investigate tolerogenic function as a developmental feature of DC and to understand molecular events involved in IL-4 priming for maturation...
  15. pmc Bi-specific MHC heterodimers for characterization of cross-reactive T cells
    Zu T Shen
    Department of Pathology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    J Biol Chem 285:33144-53. 2010
    ..A similar strategy could be used to develop reagents to analyze cross-reactive T cell responses in humans...
  16. ncbi request reprint MHC class II compartment subtypes: structure and function
    Lawrence J Stern
    Department of Pathology University of Massachusetts Medical School Worcester, MA 01655, USA
    Curr Opin Immunol 18:64-9. 2006
    ..Together, these findings begin to shed light on the connection between MIIC protein content, MIIC morphology and MHC class II-related antigen processing...
  17. pmc Soluble peptide-MHC monomers cause activation of CD8+ T cells through transfer of the peptide to T cell MHC molecules
    Qing Ge
    Department of Biology, Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 99:13729-34. 2002
    ..The result is that T cells, acting as antigen-presenting cells, are able to activate other naive T cells...
  18. ncbi request reprint Dihydrolipoic acid as an effective cofactor for peptide methionine sulfoxide reductase in enzymatic repair of oxidative damage to both lipid-free and lipid-bound apolipoprotein a-I
    Alexander B Sigalov
    Biomedical Department, AMW Biomed, 22 1 11 Tarusskaya Street, Moscow 117588, Russia
    Antioxid Redox Signal 4:553-7. 2002
    ....
  19. ncbi request reprint Exploration of the P6/P7 region of the peptide-binding site of the human class II major histocompatability complex protein HLA-DR1
    Zarixia Zavala-Ruiz
    Massachusetts Institute of Technology, Department of Chemistry, Cambridge, Massachusetts 02139, USA
    J Biol Chem 278:44904-12. 2003
    ..These results suggest a possible strategy for increasing the affinity of weakly immunogenic peptides that might be applicable to the development of vaccines and diagnostic reagents...
  20. ncbi request reprint Conformational variation of surface class II MHC proteins during myeloid dendritic cell differentiation accompanies structural changes in lysosomal MIIC
    Ilaria Potolicchio
    Department of Pathology Albert Einstein College of Medicine, New York, NY 10461, USA
    J Immunol 175:4935-47. 2005
    ..Ag loading can be achieved for the fraction of empty HLA-DR present in the "peptide-receptive" form. Finally, in vivo, APC-expressing surface empty HLA-DR were found in T cell areas of secondary lymphoid organs...
  21. ncbi request reprint Monoclonal antibodies specific for the empty conformation of HLA-DR1 reveal aspects of the conformational change associated with peptide binding
    Gregory J Carven
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    J Biol Chem 279:16561-70. 2004
    ..These results illuminate aspects of the structure of the empty forms and the nature of the peptide-induced conformational change...
  22. ncbi request reprint Crystallographic structure of the human leukocyte antigen DRA, DRB3*0101: models of a directional alloimmune response and autoimmunity
    Christian S Parry
    Massachusetts Institute of Technology, Department of Chemistry, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
    J Mol Biol 371:435-46. 2007
    ..This dichotomy is explored for clues to the disease...
  23. ncbi request reprint A polymorphic pocket at the P10 position contributes to peptide binding specificity in class II MHC proteins
    Zarixia Zavala-Ruiz
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Chem Biol 11:1395-402. 2004
    ..HLA-DR1 variants with polymorphic residues at these positions were produced and found to exhibit different side chain specificity at the P10 position. These results define a new specificity position in HLA-DR proteins...
  24. ncbi request reprint Induced fit of an epitope peptide to a monoclonal antibody probed with a novel parallel surface plasmon resonance assay
    Rick Baggio
    HTS Biosystems, East Hartford, Connecticut 06108, USA
    J Biol Chem 280:4188-94. 2005
    ..The kinetic details of the present study support the presence of a structural component in the antigenic epitope recognized by MEM-265 in the peptide-free form of major histocompatibility complex II DR1 beta-chain...
  25. ncbi request reprint Major histocompatibility complex and T cell interactions of a universal T cell epitope from Plasmodium falciparum circumsporozoite protein
    Carlos Parra-Lopez
    Fundacion Instituto de Inmunologia de Colombia, Grupo Funcional InmunologĂ­a, Carrera 50 No 26 00, Bogota, Colombia
    J Biol Chem 281:14907-17. 2006
    ..T*-1 overlaps a region of the protein that has been described as highly polymorphic; however, the particular T*-1 residues required for anchoring to DR4 were highly conserved in Plasmodium sequences described to date...
  26. ncbi request reprint Noble metals strip peptides from class II MHC proteins
    Stephen L De Wall
    Institute of Chemistry and Cell Biology, Harvard Medical School, 200 Longwood Ave, Boston, Massachusetts 02115, USA
    Nat Chem Biol 2:197-201. 2006
    ..This previously unknown allosteric mechanism may help resolve how gold(I) drugs affect the progress of rheumatoid arthritis and may provide a basis for developing a new class of anti-autoimmune drugs...
  27. ncbi request reprint CD8 T cells, like CD4 T cells, are triggered by multivalent engagement of TCRs by MHC-peptide ligands but not by monovalent engagement
    Jennifer D Stone
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    J Immunol 176:1498-505. 2006
    ..Thus, the valency dependence of the response of CD8 T cells to soluble MHC-peptide reagents is the same as previously observed for CD4 T cells...
  28. ncbi request reprint Granulocyte-macrophage colony-stimulating factor induces an expression program in neonatal microglia that primes them for antigen presentation
    Fabio Re
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Department of Medicine, Brigham and Women s Hospital and Harvard Medical School, Boston, MA 02115, USA
    J Immunol 169:2264-73. 2002
    ..This function of GM-CSF is distinct from its primary function in cell proliferation and survival...

Research Grants20

  1. Structure and Function of Class II MHC Proteins
    Lawrence Stern; Fiscal Year: 2006
    ..abstract_text> ..
  2. Interaction of HIV nef with its receptor binding partners
    Lawrence Stern; Fiscal Year: 2007
    ..This information will promote efforts to understand how nef modifies the signaling machinery in an infected cell, and to develop therapeutic approaches to interfere with this aspect of HIV infection and pathogenesis. ..
  3. Structure and Function of Class II MHC Proteins
    Lawrence Stern; Fiscal Year: 2007
    ..abstract_text> ..
  4. Class II MHC Antigen Processing in Dendritic Cells
    Lawrence Stern; Fiscal Year: 2009
    ....
  5. EMPTY CLASS II MHC ON DENDRITIC CELLS AND MICROGILIA
    Lawrence Stern; Fiscal Year: 2005
    ..This project supports that goal by focusing on biochemical aspects of antigen processing and peptide loading in dendritic cells and microglia. The companion project focuses on MHC trafficking and antigen presentation. ..
  6. Class II MHC Antigen Processing in Dendritic Cells
    Lawrence J Stern; Fiscal Year: 2010
    ....