Research Topics
| Lawrence SternSummaryAffiliation: University of Massachusetts Medical School Country: USA Publications
Research Grants
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Detail Information
Publications
Human CD4+ T cell response to human herpesvirus 6Maria D Nastke
Department of Pathology, University of Massachusetts Medical School, Worcester, Massachusetts, USA
J Virol 86:4776-92. 2012..These findings provide a window into the immune response to HHV-6 and provide a basis for tracking HHV-6 cellular immune responses...
HLA-DR: molecular insights and vaccine designLawrence J Stern
Department of Pathology, Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01655, USA
Curr Pharm Des 15:3249-61. 2009..Finally, using malaria as a model, we describe the development of a minimal subunit vaccine for the human malaria parasite Plasmodium falciparum...- Characterizing MHC-associated peptides by mass spectrometryLawrence J Stern
Department of Pathology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA
J Immunol 179:2667-8. 2007 - Homooligomerization of the cytoplasmic domain of the T cell receptor zeta chain and of other proteins containing the immunoreceptor tyrosine-based activation motifAlexander Sigalov
Department of Pathology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, Massachusetts 01655, USA
Biochemistry 43:2049-61. 2004..Phosphorylation of zeta(cyt) and FcepsilonRIgamma(cyt) does not significantly alter their oligomerization behavior. The implications of these results for transmembrane signaling and cellular activation by immune receptors are discussed... - The intrinsically disordered cytoplasmic domain of the T cell receptor zeta chain binds to the nef protein of simian immunodeficiency virus without a disorder-to-order transitionAlexander B Sigalov
Department of Pathology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, Massachusetts 01655, USA
Biochemistry 47:12942-4. 2008..Thus, our findings oppose the generally accepted view of the behavior of intrinsically disordered proteins and provide new evidence of the existence of specific interactions for unfolded protein molecules... - Lipid-binding activity of intrinsically unstructured cytoplasmic domains of multichain immune recognition receptor signaling subunitsAlexander B Sigalov
Department of Pathology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, Massachusetts 01655, USA
Biochemistry 45:15731-9. 2006..Phosphorylation of zetacyt and FcepsilonRIgammacyt does not alter their random-coil conformation but weakens binding to membranes. The implications of these results for transmembrane signal transduction by immune receptors are discussed... - Pseudo-merohedral twinning and noncrystallographic symmetry in orthorhombic crystals of SIVmac239 Nef core domain bound to different-length TCRzeta fragmentsWalter M Kim
University of Massachusetts Medical School, USA
Acta Crystallogr D Biol Crystallogr 66:163-75. 2010..The combination of NCS rotations that were nearly parallel to the twin operation (k, h, -l) and a and b unit-cell parameters that were nearly identical predisposed the P2(1)2(1)2(1) crystal form to pseudo-merohedral twinning... - Fluorogenic probes for monitoring peptide binding to class II MHC proteins in living cellsPrasanna Venkatraman
Department of Pathology, University of Massachusetts Medical School, 55 Lake Ave North, Worcester, Massachusetts 01655, USA
Nat Chem Biol 3:222-8. 2007..Using these probes we have tracked a developmentally regulated cell-surface peptide-binding activity in primary human monocyte-derived dendritic cells... - Vaccinia peptides eluted from HLA-DR1 isolated from virus-infected cells are recognized by CD4+ T cells from a vaccinated donorIwona Strug
Department of Pathology, Proteomic and Mass Spectrometry Core Facility, Center for Infectious Disease and Vaccine Research, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
J Proteome Res 7:2703-11. 2008..The peptides, derived from the I6L, D6R, and A10L viral proteins, were 15 residues in length, bound efficiently to HLA-DR1 as synthetic peptides, and were recognized by vaccinia-specific CD4 (+) T cells obtained from an immunized donor... - Human CD4+ T cell epitopes from vaccinia virus induced by vaccination or infectionJ Mauricio Calvo Calle
Department of Pathology, University of Massachusetts Medical School, Worcester, Massachusetts, USA
PLoS Pathog 3:1511-29. 2007..Moreover, the epitope identification approach developed here should find application to other large-genome pathogens... - Class II major histocompatibility complex tetramer staining: progress, problems, and prospectsSabrina S Vollers
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA, USA
Immunology 123:305-13. 2008..In this review we discuss which problems in class II MHC tetramer staining have been solved to date, and which matters remain to be considered... - Human cytotoxic CD4+ T cells recognize HLA-DR1-restricted epitopes on vaccinia virus proteins A24R and D1R conserved among poxvirusesShibani Mitra Kaushik
Center for Infectious Disease and Vaccine Research, University of Massachusetts Medical School, Worcester, MA 01655, USA
J Immunol 179:1303-12. 2007.... - Model for the peptide-free conformation of class II MHC proteinsCorrie A Painter
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts, United States of America
PLoS ONE 3:e2403. 2008..Major histocompatibility complex proteins are believed to undergo significant conformational changes concomitant with peptide binding, but structural characterization of these changes has remained elusive... 
GMCSF in the absence of other cytokines sustains human dendritic cell precursors with T cell regulatory activity and capacity to differentiate into functional dendritic cellsSriram Chitta
University of Massachusetts Medical School, Department of Pathology, Worcester, MA 10655, United States
Immunol Lett 116:41-54. 2008..We suggest that these cells may provide a useful model to investigate tolerogenic function as a developmental feature of DC and to understand molecular events involved in IL-4 priming for maturation...- Bi-specific MHC heterodimers for characterization of cross-reactive T cellsZu T Shen
Department of Pathology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
J Biol Chem 285:33144-53. 2010..A similar strategy could be used to develop reagents to analyze cross-reactive T cell responses in humans... - MHC class II compartment subtypes: structure and functionLawrence J Stern
Department of Pathology University of Massachusetts Medical School Worcester, MA 01655, USA
Curr Opin Immunol 18:64-9. 2006..Together, these findings begin to shed light on the connection between MIIC protein content, MIIC morphology and MHC class II-related antigen processing... - Soluble peptide-MHC monomers cause activation of CD8+ T cells through transfer of the peptide to T cell MHC moleculesQing Ge
Department of Biology, Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
Proc Natl Acad Sci U S A 99:13729-34. 2002..The result is that T cells, acting as antigen-presenting cells, are able to activate other naive T cells... - Dihydrolipoic acid as an effective cofactor for peptide methionine sulfoxide reductase in enzymatic repair of oxidative damage to both lipid-free and lipid-bound apolipoprotein a-IAlexander B Sigalov
Biomedical Department, AMW Biomed, 22 1 11 Tarusskaya Street, Moscow 117588, Russia
Antioxid Redox Signal 4:553-7. 2002.... - Exploration of the P6/P7 region of the peptide-binding site of the human class II major histocompatability complex protein HLA-DR1Zarixia Zavala-Ruiz
Massachusetts Institute of Technology, Department of Chemistry, Cambridge, Massachusetts 02139, USA
J Biol Chem 278:44904-12. 2003..These results suggest a possible strategy for increasing the affinity of weakly immunogenic peptides that might be applicable to the development of vaccines and diagnostic reagents... - Conformational variation of surface class II MHC proteins during myeloid dendritic cell differentiation accompanies structural changes in lysosomal MIICIlaria Potolicchio
Department of Pathology Albert Einstein College of Medicine, New York, NY 10461, USA
J Immunol 175:4935-47. 2005..Ag loading can be achieved for the fraction of empty HLA-DR present in the "peptide-receptive" form. Finally, in vivo, APC-expressing surface empty HLA-DR were found in T cell areas of secondary lymphoid organs... - Monoclonal antibodies specific for the empty conformation of HLA-DR1 reveal aspects of the conformational change associated with peptide bindingGregory J Carven
Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
J Biol Chem 279:16561-70. 2004..These results illuminate aspects of the structure of the empty forms and the nature of the peptide-induced conformational change... - Crystallographic structure of the human leukocyte antigen DRA, DRB3*0101: models of a directional alloimmune response and autoimmunityChristian S Parry
Massachusetts Institute of Technology, Department of Chemistry, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
J Mol Biol 371:435-46. 2007..This dichotomy is explored for clues to the disease... - A polymorphic pocket at the P10 position contributes to peptide binding specificity in class II MHC proteinsZarixia Zavala-Ruiz
Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
Chem Biol 11:1395-402. 2004..HLA-DR1 variants with polymorphic residues at these positions were produced and found to exhibit different side chain specificity at the P10 position. These results define a new specificity position in HLA-DR proteins... - Induced fit of an epitope peptide to a monoclonal antibody probed with a novel parallel surface plasmon resonance assayRick Baggio
HTS Biosystems, East Hartford, Connecticut 06108, USA
J Biol Chem 280:4188-94. 2005..The kinetic details of the present study support the presence of a structural component in the antigenic epitope recognized by MEM-265 in the peptide-free form of major histocompatibility complex II DR1 beta-chain... - Major histocompatibility complex and T cell interactions of a universal T cell epitope from Plasmodium falciparum circumsporozoite proteinCarlos Parra-Lopez
Fundacion Instituto de Inmunologia de Colombia, Grupo Funcional Inmunología, Carrera 50 No 26 00, Bogota, Colombia
J Biol Chem 281:14907-17. 2006..T*-1 overlaps a region of the protein that has been described as highly polymorphic; however, the particular T*-1 residues required for anchoring to DR4 were highly conserved in Plasmodium sequences described to date... - Noble metals strip peptides from class II MHC proteinsStephen L De Wall
Institute of Chemistry and Cell Biology, Harvard Medical School, 200 Longwood Ave, Boston, Massachusetts 02115, USA
Nat Chem Biol 2:197-201. 2006..This previously unknown allosteric mechanism may help resolve how gold(I) drugs affect the progress of rheumatoid arthritis and may provide a basis for developing a new class of anti-autoimmune drugs... - CD8 T cells, like CD4 T cells, are triggered by multivalent engagement of TCRs by MHC-peptide ligands but not by monovalent engagementJennifer D Stone
Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
J Immunol 176:1498-505. 2006..Thus, the valency dependence of the response of CD8 T cells to soluble MHC-peptide reagents is the same as previously observed for CD4 T cells... - Granulocyte-macrophage colony-stimulating factor induces an expression program in neonatal microglia that primes them for antigen presentationFabio Re
Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Department of Medicine, Brigham and Women s Hospital and Harvard Medical School, Boston, MA 02115, USA
J Immunol 169:2264-73. 2002..This function of GM-CSF is distinct from its primary function in cell proliferation and survival...
Research Grants
- Structure and Function of Class II MHC ProteinsLawrence Stern; Fiscal Year: 2006..abstract_text> ..
- Interaction of HIV nef with its receptor binding partnersLawrence Stern; Fiscal Year: 2007..This information will promote efforts to understand how nef modifies the signaling machinery in an infected cell, and to develop therapeutic approaches to interfere with this aspect of HIV infection and pathogenesis. ..
- Structure and Function of Class II MHC ProteinsLawrence Stern; Fiscal Year: 2007..abstract_text> ..
- Class II MHC Antigen Processing in Dendritic CellsLawrence Stern; Fiscal Year: 2009....
- EMPTY CLASS II MHC ON DENDRITIC CELLS AND MICROGILIALawrence Stern; Fiscal Year: 2005..This project supports that goal by focusing on biochemical aspects of antigen processing and peptide loading in dendritic cells and microglia. The companion project focuses on MHC trafficking and antigen presentation. ..
- Class II MHC Antigen Processing in Dendritic CellsLawrence J Stern; Fiscal Year: 2010....