R S Stephens

Summary

Affiliation: University of California
Country: USA

Publications

  1. ncbi request reprint The cellular paradigm of chlamydial pathogenesis
    Richard S Stephens
    Division of Infectious Diseases, School of Public Health, 140 Earl Warren Hall, University of California, Berkeley 94720, USA
    Trends Microbiol 11:44-51. 2003
  2. pmc Eukaryotic cell uptake of heparin-coated microspheres: a model of host cell invasion by Chlamydia trachomatis
    R S Stephens
    Program in Infectious Diseases, School of Public Health, University of California, Berkeley, California 94720, USA
    Infect Immun 68:1080-5. 2000
  3. pmc ChxR is a transcriptional activator in Chlamydia
    Ingrid Chou Koo
    Program in Infectious Diseases, School of Public Health, University of California, Berkeley, CA 94720 7360, USA
    Proc Natl Acad Sci U S A 103:750-5. 2006
  4. pmc Interaction of Chlamydia trachomatis with mammalian cells is independent of host cell surface heparan sulfate glycosaminoglycans
    Richard S Stephens
    Division of Infectious Diseases, School of Public Health, 140 Earl Warren Hall, University of California, Berkeley, CA 94720, USA
    Infect Immun 74:1795-9. 2006
  5. ncbi request reprint Activation of the host cell proinflammatory interleukin-8 response by Chlamydia trachomatis
    Kerry R Buchholz
    Program in Infectious Diseases and Immunity, University of California, Berkeley, CA 94720, USA
    Cell Microbiol 8:1768-79. 2006
  6. ncbi request reprint Heparin-binding outer membrane protein of chlamydiae
    R S Stephens
    Division of Infectious Diseases, School of Public Health, 235 Earl Warren Hall, University of California, Berkeley, CA 94720, USA
    Mol Microbiol 40:691-9. 2001
  7. ncbi request reprint Chlamydial genomics and vaccine antigen discovery
    R S Stephens
    Program in Infectious Diseases, University of California, Berkeley, CA 94720 7360
    J Infect Dis 181:S521-3. 2000
  8. ncbi request reprint Genome sequence of an obligate intracellular pathogen of humans: Chlamydia trachomatis
    R S Stephens
    Program in Infectious Diseases, University of California, Berkeley, CA 94720, USA
    Science 282:754-9. 1998
  9. pmc Identification by sequence analysis of two-site posttranslational processing of the cysteine-rich outer membrane protein 2 of Chlamydia trachomatis serovar L2
    J E Allen
    Department of Biomedical and Environmental Health Science, University of California, Berkeley 94720
    J Bacteriol 171:285-91. 1989
  10. pmc Secretion of proinflammatory cytokines by epithelial cells in response to Chlamydia infection suggests a central role for epithelial cells in chlamydial pathogenesis
    S J Rasmussen
    Francis I Proctor Foundation, University of California, San Francisco 94143, USA
    J Clin Invest 99:77-87. 1997

Collaborators

  • J Schachter
  • J E Allen
  • J Fierer
  • D J Anderson
  • L Eckmann
  • Q Zhao
  • D L Bain
  • R W Davis
  • J Z Zhang
  • Lakshminarayan M Iyer
  • E V Koonin
  • L Aravind
  • A J Quayle
  • Kerry R Buchholz
  • P Scott Hefty
  • Stephanie Abromaitis
  • Tracy L Nicholson
  • Kevin Hybiske
  • Betsy Kleba
  • Ingrid Chou Koo
  • Diane E Kawa
  • Erika A Lindquist
  • Betsy J Kleba
  • Nicholas R Thomson
  • Carolyn G Conant
  • Lei Hua
  • Brian W Brunelle
  • Lynn Olinger
  • Trevor Fudyk
  • J Grimwood
  • A Kubo
  • Caroline Carder
  • Ian N Clarke
  • Paul Skipp
  • C David O'Connor
  • Michael A Quail
  • Pete Marsh
  • Nicola Lennard
  • Julian Parkhill
  • Richard Rance
  • Barbara Harris
  • Doug Ormond
  • Sarah J Lockey
  • Halina Norbertzcak
  • Ian Goodhead
  • Matthew T G Holden
  • Chester W Price
  • Young Jin Lee
  • Don Walthers
  • Young Moo Lee
  • Linda J Kenney
  • Karen Chiu
  • S J Rasmussen
  • Kimberley Chong
  • Gary Schoolnik
  • James D Marks
  • Erin Banta
  • L Olinger
  • Y X Zhang
  • L Shen
  • M F Kagnoff

Detail Information

Publications39

  1. ncbi request reprint The cellular paradigm of chlamydial pathogenesis
    Richard S Stephens
    Division of Infectious Diseases, School of Public Health, 140 Earl Warren Hall, University of California, Berkeley 94720, USA
    Trends Microbiol 11:44-51. 2003
    ....
  2. pmc Eukaryotic cell uptake of heparin-coated microspheres: a model of host cell invasion by Chlamydia trachomatis
    R S Stephens
    Program in Infectious Diseases, School of Public Health, University of California, Berkeley, California 94720, USA
    Infect Immun 68:1080-5. 2000
    ..These findings suggest that heparin-coated beads and Chlamydia trachomatis enter eukaryotic cells by similar pathways...
  3. pmc ChxR is a transcriptional activator in Chlamydia
    Ingrid Chou Koo
    Program in Infectious Diseases, School of Public Health, University of California, Berkeley, CA 94720 7360, USA
    Proc Natl Acad Sci U S A 103:750-5. 2006
    ..Both proteins could bind simultaneously to their nonoverlapping binding sites. This report identifies a stage-specific transcriptional regulator and some of its target genes in Chlamydia...
  4. pmc Interaction of Chlamydia trachomatis with mammalian cells is independent of host cell surface heparan sulfate glycosaminoglycans
    Richard S Stephens
    Division of Infectious Diseases, School of Public Health, 140 Earl Warren Hall, University of California, Berkeley, CA 94720, USA
    Infect Immun 74:1795-9. 2006
    ..These data do not support the hypothesis and demonstrate that host cell surface heparan sulfate does not serve an essential functional role in chlamydial infectivity...
  5. ncbi request reprint Activation of the host cell proinflammatory interleukin-8 response by Chlamydia trachomatis
    Kerry R Buchholz
    Program in Infectious Diseases and Immunity, University of California, Berkeley, CA 94720, USA
    Cell Microbiol 8:1768-79. 2006
    ..trachomatis. We conclude that a C. trachomatis growth-dependent factor produced at mid-developmental stage induces IL-8 within the epithelial cell it infects through activation of host signalling pathways...
  6. ncbi request reprint Heparin-binding outer membrane protein of chlamydiae
    R S Stephens
    Division of Infectious Diseases, School of Public Health, 235 Earl Warren Hall, University of California, Berkeley, CA 94720, USA
    Mol Microbiol 40:691-9. 2001
    ..Surface localization of OmcB was shown using monospecific antisera specific to the 20-mer OmcB peptide that bound the surfaces of elementary bodies (EB) and by heparin-binding peptide cross-linking of EB surface proteins...
  7. ncbi request reprint Chlamydial genomics and vaccine antigen discovery
    R S Stephens
    Program in Infectious Diseases, University of California, Berkeley, CA 94720 7360
    J Infect Dis 181:S521-3. 2000
    ..This knowledge will aid vaccine antigen discovery by facilitating the identification, testing, and evaluation of antigens by way of vaccine delivery approaches that elicit protective immunity...
  8. ncbi request reprint Genome sequence of an obligate intracellular pathogen of humans: Chlamydia trachomatis
    R S Stephens
    Program in Infectious Diseases, University of California, Berkeley, CA 94720, USA
    Science 282:754-9. 1998
    ..The phylogenetic mosaic of chlamydial genes, including a large number of genes with phylogenetic origins from eukaryotes, implies a complex evolution for adaptation to obligate intracellular parasitism...
  9. pmc Identification by sequence analysis of two-site posttranslational processing of the cysteine-rich outer membrane protein 2 of Chlamydia trachomatis serovar L2
    J E Allen
    Department of Biomedical and Environmental Health Science, University of California, Berkeley 94720
    J Bacteriol 171:285-91. 1989
    ..The mature proteins had a net positive charge and contained 24 cysteine residues...
  10. pmc Secretion of proinflammatory cytokines by epithelial cells in response to Chlamydia infection suggests a central role for epithelial cells in chlamydial pathogenesis
    S J Rasmussen
    Francis I Proctor Foundation, University of California, San Francisco 94143, USA
    J Clin Invest 99:77-87. 1997
    ..These findings suggest a novel pathophysiologic concept wherein the acute host response to Chlamydia at mucosal surfaces is primarily initiated and sustained by epithelial cells, the first and major targets of chlamydial infection...
  11. ncbi request reprint Chlamydia outer membrane protein discovery using genomics
    R S Stephens
    Division of Infectious Diseases, School of Public Health, University of California, Berkeley, California 94720, USA
    Curr Opin Microbiol 4:16-20. 2001
    ..Several of the predicted outer membrane proteins recently have been shown to be translated and localized to the surface of the chlamydial outer membrane...
  12. ncbi request reprint Chlamydial genovar distribution after community wide antibiotic treatment
    D L Bain
    Division of Infectious Diseases, University of California, Berkeley, CA 94720-7360, USA
    J Infect Dis 184:1581-8. 2001
    ..Evidence consistent with reinfection and persistence as mechanisms of communitywide continued presence of trachoma was provided by data for individuals infected with rare genovars...
  13. doi request reprint Divergence without difference: phylogenetics and taxonomy of Chlamydia resolved
    Richard S Stephens
    University of California, Berkeley, USA
    FEMS Immunol Med Microbiol 55:115-9. 2009
    ....
  14. ncbi request reprint Substrate-specific diffusion of select dicarboxylates through Chlamydia trachomatis PorB
    A Kubo
    Division of Infectious Diseases, School of Public Health, 140 Earl Warren Hall, University of California, Berkeley, CA 94720, USA
    Microbiology 147:3135-40. 2001
    ..These data demonstrate that PorB is a dicarboxylate-specific porin that may feed the chlamydial TCA cycle and provide chlamydiae with carbon and energy production intermediates...
  15. pmc Expression of Chlamydia pneumoniae polymorphic membrane protein family genes
    J Grimwood
    Francis I. Proctor Foundation, University of California, San Francisco, California, USA
    Infect Immun 69:2383-9. 2001
    ..Analyses of additional C. pneumoniae strains showed that although some Pmps are conserved, others vary between strains, in both molecular weight and level of expression...
  16. pmc Developmental regulation of tandem promoters for the major outer membrane protein gene of Chlamydia trachomatis
    R S Stephens
    Department of Biomedical and Environmental Health Science, School of Public Health, University of California, Berkeley 94720
    J Bacteriol 170:744-50. 1988
    ..Mapping of the initiating nucleotide for each transcript suggested that this gene is regulated by differential transcription from tandem promoters...
  17. pmc Microarray-based genomic surveying of gene polymorphisms in Chlamydia trachomatis
    Brian W Brunelle
    Program in Infectious Diseases, University of California, Berkeley, CA 94720 7360, USA
    Genome Biol 5:R42. 2004
    ..Variable genes within 14 uncharacterized strains of C. trachomatis were identified by array analysis and verified by DNA sequencing. These genes may be crucial for understanding chlamydial virulence and pathogenesis...
  18. pmc Attachment and entry of Chlamydia have distinct requirements for host protein disulfide isomerase
    Stephanie Abromaitis
    Program in Infectious Diseases and Immunity, University of California Berkeley, Berkeley, CA, USA
    PLoS Pathog 5:e1000357. 2009
    ....
  19. ncbi request reprint Cysteine-rich outer membrane proteins of Chlamydia trachomatis display compensatory sequence changes between biovariants
    J E Allen
    Department of Biomedical and Environmental Health Sciences, University of California, Berkeley 94720
    Mol Microbiol 4:1543-50. 1990
    ..These compensatory charge alterations have implications for the outer membrane organization of these proteins. In addition, examination of the OMP3 sequence suggests that OMP3 may be a lipoprotein...
  20. ncbi request reprint Chlamydia attachment to mammalian cells requires protein disulfide isomerase
    Carolyn G Conant
    Division of Infectious Diseases, School of Public Health, 140 Earl Warren Hall, University of California, Berkeley, CA 94720, USA
    Cell Microbiol 9:222-32. 2007
    ..The cell line was also resistant to diphtheria toxin and required complemented cell-surface PDI for toxin entry. These data demonstrate that native PDI at the cell surface is required for effective chlamydial attachment and infectivity...
  21. pmc Mechanisms of Chlamydia trachomatis entry into nonphagocytic cells
    Kevin Hybiske
    Division of Infectious Diseases, School of Public Health, 140 Warren Hall, University of California Berkeley, Berkeley, CA 94720, USA
    Infect Immun 75:3925-34. 2007
    ..Thus, clathrin and its coordinate accessory factors were required for entry of C. trachomatis, although additional, uncharacterized mechanisms are also utilized...
  22. ncbi request reprint Antigenic topology of chlamydial PorB protein and identification of targets for immune neutralization of infectivity
    Diane E Kawa
    Division of Infectious Diseases, School of Public Health, 235 Warren Hall, University of California, Berkeley, CA 94720, USA
    J Immunol 168:5184-91. 2002
    ..trachomatis PorB antisera was inhibited by synthetic peptides representing the surface-exposed PorB antigenic determinants. These findings demonstrate that PorB Ags may be useful for development of chlamydial vaccines...
  23. pmc Mechanisms of host cell exit by the intracellular bacterium Chlamydia
    Kevin Hybiske
    Division of Infectious Diseases, School of Public Health, University of California, Berkeley, CA 94720, USA
    Proc Natl Acad Sci U S A 104:11430-5. 2007
    ..The participation of Rho was unique in that it functioned late in extrusion. The dual nature of release characterized for Chlamydia has not been observed as a strategy for intracellular bacteria...
  24. pmc The extracellular signal-regulated kinase/mitogen-activated protein kinase pathway induces the inflammatory factor interleukin-8 following Chlamydia trachomatis infection
    Kerry R Buchholz
    Program in Infectious Diseases and Immunity, School of Public Health, University of California Berkeley, Berkeley, CA 94720, USA
    Infect Immun 75:5924-9. 2007
    ..C. trachomatis serovar D also induced IL-8 in an ERK-dependent manner. We concluded that IL-8 induced during infection of epithelial cells is dependent on continual activation of ERK by C. trachomatis...
  25. pmc The cytosolic pattern recognition receptor NOD1 induces inflammatory interleukin-8 during Chlamydia trachomatis infection
    Kerry R Buchholz
    Program in Infectious Diseases and Immunity, University of California, Berkeley, CA 94720 7354, USA
    Infect Immun 76:3150-5. 2008
    ..Since ERK is not activated through this pathway, a concomitant interaction between the host and bacteria is additionally required for full activation of the endogenous IL-8 response...
  26. ncbi request reprint Immune response to the Chlamydia trachomatis outer membrane protein PorB
    Diane E Kawa
    Program in Infectious Diseases, School of Public Health, University of California, 140 Earl Warren Hall, Berkeley, CA 94720, USA
    Vaccine 22:4282-6. 2004
    ....
  27. pmc Chlamydial effector proteins localized to the host cell cytoplasmic compartment
    Betsy Kleba
    Program in Infectious Diseases and Immunity, University of California, Berkeley, CA 94720, USA
    Infect Immun 76:4842-50. 2008
    ..These data confirm that a dynamic relationship exists between Chlamydia and its host and that translocation of bacterial proteins into the cytosol is developmentally dependent...
  28. pmc Selection of mutant cell lines resistant to infection by Chlamydia spp [corrected]
    Trevor Fudyk
    Division of Infectious Diseases, School of Public Health, University of California, Berkeley, California 94720, USA
    Infect Immun 70:6444-7. 2002
    ..Four distinct mutant cell phenotypes with defects in either attachment or postattachment mechanisms that are required for infection by C. trachomatis and Chlamydia pneumoniae were characterized...
  29. pmc Chlamydia pneumoniae encodes a functional aromatic amino acid hydroxylase
    Stephanie Abromaitis
    University of California, Berkeley, 94720, USA
    FEMS Immunol Med Microbiol 55:196-205. 2009
    ..The maintenance of this gene within a number of Chlamydia suggests that the enzyme may have an important role in shaping the metabolism or overall pathogenesis of these bacteria...
  30. ncbi request reprint Phage-display antibody detection of Chlamydia trachomatis-associated antigens
    Erika A Lindquist
    Division of Infectious Diseases, School of Public Health, 235 Earl Warren Hall, University of California, Berkeley, CA 94720 7360, USA
    Microbiology 148:443-51. 2002
    ....
  31. pmc Chlamydial type III secretion system is encoded on ten operons preceded by sigma 70-like promoter elements
    P Scott Hefty
    Division of Infectious Diseases, School of Public Health, 140 Earl Warren Hall, University of California, Berkeley, Berkeley, CA 94720, USA
    J Bacteriol 189:198-206. 2007
    ..Transcriptional initiation by a sigma factor responsible for constitutive gene expression indicates that undefined activators or repressors regulate developmental stage-specific expression of chlamydial type III secretion system genes...
  32. ncbi request reprint A developmentally regulated two-component signal transduction system in Chlamydia
    Ingrid Chou Koo
    Division of Infectious Diseases, School of Public Health, University of California, Berkeley, California 94720 7360, USA
    J Biol Chem 278:17314-9. 2003
    ..Collectively, these results show that CtcB and CtcC function as a two-component system and are likely responsible for transcriptional regulation by sigma(54) holoenzyme during late-stage chlamydial development...
  33. pmc Global stage-specific gene regulation during the developmental cycle of Chlamydia trachomatis
    Tracy L Nicholson
    Division of Infectious Diseases, School of Public Health, University of California, Berkeley, California 94720, USA
    J Bacteriol 185:3179-89. 2003
    ..The correlation of these gene clusters with hallmark morphological events of the chlamydial developmental cycle suggests three global stage-specific networks of gene regulation...
  34. pmc Recruitment of mammalian cell fibronectin to the surface of Chlamydia trachomatis
    Betsy J Kleba
    Division of Infectious Diseases, School of Public Health, University of California, Berkeley, California 94720, USA
    Infect Immun 70:3935-8. 2002
    ..We also demonstrate that a heparan sulfate lyase-sensitive molecule on chlamydial EB is responsible for binding at least a portion of this fibronectin...
  35. pmc Chlamydia trachomatis lacks an adaptive response to changes in carbon source availability
    Tracy L Nicholson
    Program in Infectious Diseases, School of Public Health, University of California Berkeley, 140 Earl Warren Hall, Berkeley, CA 94720 7360, USA
    Infect Immun 72:4286-9. 2004
    ..In contrast to free-living bacteria, changing the carbon source from glucose to glutamate or alpha-ketoglutarate had little effect on the global gene transcription of C. trachomatis...
  36. ncbi request reprint Bacteria-associated fibronectin does not enhance Chlamydia trachomatis infectivity in vitro
    Betsy Kleba
    Program in Infectious Diseases and Immunity, University of California, Berkeley, CA 94720, USA
    Microb Pathog 39:53-5. 2005
    ..However, at high fibronectin concentrations (1 mg/ml) bacterial infectivity was reduced. These data suggest that, unlike several other microbial pathogens, C. trachomatis does not utilize fibronectin to mediate infectivity in vitro...
  37. ncbi request reprint Core of the partner switching signalling mechanism is conserved in the obligate intracellular pathogen Chlamydia trachomatis
    Lei Hua
    Department of Food Science and Technology, University of California, Davis, 95616, USA
    Mol Microbiol 59:623-36. 2006
    ..trachomatis cytoplasmic membrane...
  38. ncbi request reprint Genetic diversity of Chlamydia trachomatis and the prevalence of trachoma
    Jianzhi Zhang
    Francis I Proctor Foundation, University of California, San Francisco, CA 94143 0944, USA
    Pediatr Infect Dis J 23:217-20. 2004
    ..We determined the predominant strains of ocular C. trachomatis in trachoma-endemic villages of Nepal and tested the hypothesis that strain diversity is associated with the prevalence of infection...
  39. pmc Chlamydia trachomatis: genome sequence analysis of lymphogranuloma venereum isolates
    Nicholas R Thomson
    The Pathogen Sequencing Unit, The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SA, UK
    Genome Res 18:161-71. 2008
    ..We have used new sequencing technologies to show that the recent clinical LGV isolate causing proctitis is unlikely to be a newly emerged strain but is most probably an old strain with relatively new clinical manifestations...