Earle Stellwagen

Summary

Affiliation: University of Iowa
Country: USA

Publications

  1. ncbi Protein folding observed by capillary electrophoresis in isoelectric buffers
    E Stellwagen
    Department of Biochemistry, University of Iowa, Iowa City 52242, USA
    J Chromatogr A 838:131-8. 1999
  2. ncbi Salt-promoted protein folding, preferential binding, or electrostatic screening?
    Earle Stellwagen
    Department of Biochemistry, University of Iowa, Iowa City, Iowa 52242, USA
    Proteins 49:147-53. 2002
  3. ncbi Analysis of the isomeric composition of the proline peptide bond in an angiotensin-converting enzyme inhibitor using capillary electrophoresis
    Earle Stellwagen
    Department of Biochemistry, University of Iowa, Iowa City, IA 52242, USA
    Anal Biochem 321:167-73. 2003
  4. ncbi Electrophoretic mobility is a reporter of hairpin structure in single-stranded DNA oligomers
    Earle Stellwagen
    Department of Biochemistry, University of Iowa, Iowa City, Iowa 52242, USA
    Biochemistry 46:10931-41. 2007
  5. pmc Quantitative analysis of monovalent counterion binding to random-sequence, double-stranded DNA using the replacement ion method
    Earle Stellwagen
    Department of Biochemistry, University of Iowa, Iowa City, Iowa 52242, USA
    Biochemistry 46:2050-8. 2007
  6. pmc Probing the electrostatic shielding of DNA with capillary electrophoresis
    Earle Stellwagen
    Department of Biochemistry, University of Iowa, Iowa City 52242, USA
    Biophys J 84:1855-66. 2003
  7. ncbi Monovalent cations affect the free solution mobility of DNA by perturbing the hydrogen-bonded structure of water
    Earle Stellwagen
    Department of Biochemistry, University of Iowa, Iowa City, IA 52242, USA
    Biopolymers 78:62-8. 2005
  8. pmc DNA A-tracts are not curved in solutions containing high concentrations of monovalent cations
    Earle Stellwagen
    Department of Biochemistry, University of Iowa, Iowa City, Iowa 52242, USA
    Biochemistry 52:4138-48. 2013
  9. doi Monovalent cation size and DNA conformational stability
    Earle Stellwagen
    Department of Biochemistry, University of Iowa, Iowa City, Iowa 52242, United States
    Biochemistry 50:3084-94. 2011
  10. pmc Capillary electrophoresis is a sensitive monitor of the hairpin-random coil transition in DNA oligomers
    Earle Stellwagen
    Department of Biochemistry, University of Iowa, Iowa City, IA 52242, USA
    Anal Biochem 365:103-10. 2007

Collaborators

Detail Information

Publications23

  1. ncbi Protein folding observed by capillary electrophoresis in isoelectric buffers
    E Stellwagen
    Department of Biochemistry, University of Iowa, Iowa City 52242, USA
    J Chromatogr A 838:131-8. 1999
    ..The potential of the method is illustrated using the small globular protein cytochrome c...
  2. ncbi Salt-promoted protein folding, preferential binding, or electrostatic screening?
    Earle Stellwagen
    Department of Biochemistry, University of Iowa, Iowa City, Iowa 52242, USA
    Proteins 49:147-53. 2002
    ....
  3. ncbi Analysis of the isomeric composition of the proline peptide bond in an angiotensin-converting enzyme inhibitor using capillary electrophoresis
    Earle Stellwagen
    Department of Biochemistry, University of Iowa, Iowa City, IA 52242, USA
    Anal Biochem 321:167-73. 2003
    ..Addition of salt to the isoelectric form or addition of an aprotic solvent to any form prior to analysis increases the trans isomer composition. Similar analyses can be made using the alternative ACE inhibitors captopril and enalaprilat...
  4. ncbi Electrophoretic mobility is a reporter of hairpin structure in single-stranded DNA oligomers
    Earle Stellwagen
    Department of Biochemistry, University of Iowa, Iowa City, Iowa 52242, USA
    Biochemistry 46:10931-41. 2007
    ....
  5. pmc Quantitative analysis of monovalent counterion binding to random-sequence, double-stranded DNA using the replacement ion method
    Earle Stellwagen
    Department of Biochemistry, University of Iowa, Iowa City, Iowa 52242, USA
    Biochemistry 46:2050-8. 2007
    ..Single-stranded DNA oligomers do not bind the five tested cations under the conditions investigated here...
  6. pmc Probing the electrostatic shielding of DNA with capillary electrophoresis
    Earle Stellwagen
    Department of Biochemistry, University of Iowa, Iowa City 52242, USA
    Biophys J 84:1855-66. 2003
    ..The results suggest that the electrostatic shielding of DNA is determined not only by the relative concentrations of the various ions in the solution, but also by their equivalent conductivities...
  7. ncbi Monovalent cations affect the free solution mobility of DNA by perturbing the hydrogen-bonded structure of water
    Earle Stellwagen
    Department of Biochemistry, University of Iowa, Iowa City, IA 52242, USA
    Biopolymers 78:62-8. 2005
    ..Hence, monovalent cations modify the free solution mobility of DNA primarily by perturbing the hydrogen-bonded structure of water, affecting the friction experienced by the migrating DNA molecules during electrophoresis...
  8. pmc DNA A-tracts are not curved in solutions containing high concentrations of monovalent cations
    Earle Stellwagen
    Department of Biochemistry, University of Iowa, Iowa City, Iowa 52242, USA
    Biochemistry 52:4138-48. 2013
    ..The decrease in A-tract curvature with increasing ionic strength, along with the known decrease in A-tract curvature with increasing temperature, suggests that DNA A-tracts are not significantly curved under physiological conditions...
  9. doi Monovalent cation size and DNA conformational stability
    Earle Stellwagen
    Department of Biochemistry, University of Iowa, Iowa City, Iowa 52242, United States
    Biochemistry 50:3084-94. 2011
    ..Hydrophobic interactions between alkylammonium ions interacting electrostatically with the phosphate residues in the coil may amplify the effect of cation size on DNA thermal stability...
  10. pmc Capillary electrophoresis is a sensitive monitor of the hairpin-random coil transition in DNA oligomers
    Earle Stellwagen
    Department of Biochemistry, University of Iowa, Iowa City, IA 52242, USA
    Anal Biochem 365:103-10. 2007
    ..2M urea and to 33 degrees C in buffers containing 7.2M urea plus 40% (v/v) formamide. The combined results indicate that CE is a sensitive technique for monitoring conformational transitions in small DNA molecules...
  11. ncbi Quantitative analysis of cation binding to the adenosine nucleotides using the variable ionic strength method: validation of the Debye-Hückel-Onsager theory of electrophoresis in the absence of counterion binding
    Earle Stellwagen
    Department of Biochemistry, University of Iowa, Iowa City, IA 52242, USA
    Electrophoresis 28:1053-62. 2007
    ..Interestingly, Tris(+) and Li(+) bind to the adenosine nucleotides with approximately equal affinities, suggesting that positively charged Tris(+) buffer ions can compete with alkali metal ions in Tris-buffered solutions...
  12. pmc Monovalent cation binding in the minor groove of DNA A-tracts
    Qian Dong
    Department of Biochemistry, University of Iowa, Iowa City, Iowa 52242, USA
    Biochemistry 48:1047-55. 2009
    ....
  13. pmc Curved DNA molecules migrate anomalously slowly in free solution
    Earle Stellwagen
    Department of Biochemistry, University of Iowa, Iowa City, IA, USA
    Nucleic Acids Res 33:4425-32. 2005
    ..The relative contribution of each A- or T-tract to the observed curvature depends on its spacing with respect to the first A-tract in the curvature module...
  14. ncbi Unified description of electrophoresis and diffusion for DNA and other polyions
    Earle Stellwagen
    Department of Biochemistry, University of Iowa, Iowa City, Iowa 52242, USA
    Biochemistry 42:11745-50. 2003
    ..The average value of the ratio mu/N(m)D is 35 +/- 6 V(-1) for 14 PSS samples containing up to 855 monomers...
  15. ncbi Effect of organic cosolvents on the free solution mobility of curved and normal DNA molecules
    Yongjun Lu
    Department of Internal Medicine, University of Iowa, Iowa City, IA 52242, USA
    Electrophoresis 27:1462-70. 2006
    ....
  16. ncbi Determining the electrophoretic mobility and translational diffusion coefficients of DNA molecules in free solution
    Earle Stellwagen
    Department of Biochemistry, University of Iowa, Iowa City, IA 52242, USA
    Electrophoresis 23:2794-803. 2002
    ..The methods used to carry out these experiments are also described in detail...
  17. pmc Do zwitterions contribute to the ionic strength of a solution?
    Earle Stellwagen
    Department of Biochemistry, University of Iowa, Iowa City, IA 52242, USA
    Anal Biochem 373:407-9. 2008
    ..However, the mobility was independent of the concentration of added tricine(+/-). Hence, zwitterions do not contribute to the ionic strength of a solution...
  18. ncbi The free solution mobility of DNA and other analytes varies as the logarithm of the fractional negative charge
    Nancy C Stellwagen
    Department of Biochemistry, University of Iowa, Iowa City, IA, USA
    Electrophoresis 35:1855-63. 2014
    ....
  19. ncbi Free solution mobility of small single-stranded oligonucleotides with variable charge densities
    Qian Dong
    Department of Biochemistry, University of Iowa, Iowa City 52242, USA
    Electrophoresis 24:3323-9. 2003
    ....
  20. ncbi Inaccurate voltages reported by the Beckman/Coulter MDQ Capillary Electrophoresis System at low voltages lead to the calculation of erroneous electrophoretic mobilities
    Yongjun Lu
    Department of Biochemistry, University of Iowa, Iowa City, IA 52242, USA
    Anal Biochem 332:191-2. 2004
  21. pmc Effect of the matrix on DNA electrophoretic mobility
    Nancy C Stellwagen
    Department of Biochemistry, University of Iowa, 4403 Bowen Science Building, Iowa City, IA 52242, USA
    J Chromatogr A 1216:1917-29. 2009
    ....
  22. ncbi The free solution mobility of DNA in Tris-acetate-EDTA buffers of different concentrations, with and without added NaCl
    Earle Stellwagen
    Department of Biochemistry, University of Iowa, Iowa City, IA 52242, USA
    Electrophoresis 23:1935-41. 2002
    ..Somewhat surprisingly, the mobilities of the single- and double-stranded 20-mers are equal to each other in TAE buffers with and without added NaCl...
  23. ncbi The sensitivity of the cis/trans-isomerization of enalapril and enalaprilat to solvent conditions
    Robin Ledger
    J Pharm Pharmacol 57:845-50. 2005
    ....