CLIFFORD JOHN STEER

Summary

Affiliation: University of Minnesota
Country: USA

Publications

  1. pmc Quantitative real-time polymerase chain reaction (qRT-PCR) restriction fragment length polymorphism (RFLP) method for monitoring highly conserved transgene expression during gene therapy
    Carol M Bruzzone
    Department Medicine, University of Minnesota, Minneapolis, MN 55455, USA
    Transl Res 152:290-7. 2008
  2. pmc β-Globin sleeping beauty transposon reduces red blood cell sickling in a patient-derived CD34(+)-based in vitro model
    Lucas M Sjeklocha
    Department of Medicine, Division of Gastroenterology, Hepatology and Nutrition, University of Minnesota Medical School, Minneapolis, Minnesota, United States of America
    PLoS ONE 8:e80403. 2013
  3. pmc Erythroid-specific expression of β-globin from Sleeping Beauty-transduced human hematopoietic progenitor cells
    Lucas M Sjeklocha
    Division of Gastroenterology, Hepatology and Nutrition, Department of Medicine, University of Minnesota Medical School, Minneapolis, Minnesota, USA
    PLoS ONE 6:e29110. 2011
  4. pmc Efficient recovery of proteins from multiple source samples after TRIzol(®) or TRIzol(®)LS RNA extraction and long-term storage
    André E S Simões
    Research Institute for Medicines and Pharmaceutical Sciences iMED UL, Faculty of Pharmacy, University of Lisbon, Lisbon, Portugal
    BMC Genomics 14:181. 2013
  5. pmc Apoptosis-associated microRNAs are modulated in mouse, rat and human neural differentiation
    Marcia M Aranha
    Research Institute for Medicines and Pharmaceutical Sciences, Faculty of Pharmacy, University of Lisbon, Lisbon 1649 003, Portugal
    BMC Genomics 11:514. 2010
  6. pmc Human colon cancer profiles show differential microRNA expression depending on mismatch repair status and are characteristic of undifferentiated proliferative states
    Aaron L Sarver
    Biostatistics and Informatics, Masonic Cancer Center, University of Minnesota, Minneapolis, Minnesota, USA
    BMC Cancer 9:401. 2009
  7. pmc MicroRNAs identified in highly purified liver-derived mitochondria may play a role in apoptosis
    Betsy T Kren
    Department of Medicine, University of Minnesota Medical School, Minneapolis, Minnesota 55455, USA
    RNA Biol 6:65-72. 2009
  8. ncbi request reprint Hepatic differentiation of liver-derived progenitor cells and their characterization by microRNA analysis
    Yixin Chen
    Department of Medicine, University of Minnesota, Minneapolis, MN 55455, USA
    Liver Transpl 16:1086-97. 2010
  9. ncbi request reprint A facile method for somatic, lifelong manipulation of multiple genes in the mouse liver
    Kirk J Wangensteen
    The Arnold and Mabel Beckman Center for Transposon Research, Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN, USA
    Hepatology 47:1714-24. 2008
  10. pmc High-level genomic integration, epigenetic changes, and expression of sleeping beauty transgene
    Jianhui Zhu
    Department of Medicine, University of Minnesota Medical School, Minneapolis, Minnesota 55455, USA
    Biochemistry 49:1507-21. 2010

Collaborators

Detail Information

Publications42

  1. pmc Quantitative real-time polymerase chain reaction (qRT-PCR) restriction fragment length polymorphism (RFLP) method for monitoring highly conserved transgene expression during gene therapy
    Carol M Bruzzone
    Department Medicine, University of Minnesota, Minneapolis, MN 55455, USA
    Transl Res 152:290-7. 2008
    ..This method has translational applications to the design, delivery, and monitoring of gene-therapy vectors...
  2. pmc β-Globin sleeping beauty transposon reduces red blood cell sickling in a patient-derived CD34(+)-based in vitro model
    Lucas M Sjeklocha
    Department of Medicine, Division of Gastroenterology, Hepatology and Nutrition, University of Minnesota Medical School, Minneapolis, Minnesota, United States of America
    PLoS ONE 8:e80403. 2013
    ..8 ± 4.5% to 29.5 ± 3.0% in control versus treated (p < 0.05). These results support the potential use of Sleeping Beauty as a clinical gene therapy vector and provide a useful tool for studying sickle red blood cells in vitro. ..
  3. pmc Erythroid-specific expression of β-globin from Sleeping Beauty-transduced human hematopoietic progenitor cells
    Lucas M Sjeklocha
    Division of Gastroenterology, Hepatology and Nutrition, Department of Medicine, University of Minnesota Medical School, Minneapolis, Minnesota, USA
    PLoS ONE 6:e29110. 2011
    ..These results support the use of Sleeping Beauty vectors in carrying an insulated IHK-β-globin transgene for gene therapy of sickle cell disease...
  4. pmc Efficient recovery of proteins from multiple source samples after TRIzol(®) or TRIzol(®)LS RNA extraction and long-term storage
    André E S Simões
    Research Institute for Medicines and Pharmaceutical Sciences iMED UL, Faculty of Pharmacy, University of Lisbon, Lisbon, Portugal
    BMC Genomics 14:181. 2013
    ..These reagents are widely used for RNA and/or DNA isolation, while reports on their use for protein extraction are limited, attributable to technical difficulties in protein solubilisation...
  5. pmc Apoptosis-associated microRNAs are modulated in mouse, rat and human neural differentiation
    Marcia M Aranha
    Research Institute for Medicines and Pharmaceutical Sciences, Faculty of Pharmacy, University of Lisbon, Lisbon 1649 003, Portugal
    BMC Genomics 11:514. 2010
    ..In addition, the expression of antiapoptotic miR-19a and 20a was also evaluated...
  6. pmc Human colon cancer profiles show differential microRNA expression depending on mismatch repair status and are characteristic of undifferentiated proliferative states
    Aaron L Sarver
    Biostatistics and Informatics, Masonic Cancer Center, University of Minnesota, Minneapolis, Minnesota, USA
    BMC Cancer 9:401. 2009
    ..Differential miRNA expression in cancer versus normal tissue is a common event and may be pivotal for tumor onset and progression...
  7. pmc MicroRNAs identified in highly purified liver-derived mitochondria may play a role in apoptosis
    Betsy T Kren
    Department of Medicine, University of Minnesota Medical School, Minneapolis, Minnesota 55455, USA
    RNA Biol 6:65-72. 2009
    ..Given the central role that mitochondria play in apoptosis, the results suggest that they might serve as reservoirs of select miRNAs that may modulate these processes in a coordinate fashion...
  8. ncbi request reprint Hepatic differentiation of liver-derived progenitor cells and their characterization by microRNA analysis
    Yixin Chen
    Department of Medicine, University of Minnesota, Minneapolis, MN 55455, USA
    Liver Transpl 16:1086-97. 2010
    ....
  9. ncbi request reprint A facile method for somatic, lifelong manipulation of multiple genes in the mouse liver
    Kirk J Wangensteen
    The Arnold and Mabel Beckman Center for Transposon Research, Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN, USA
    Hepatology 47:1714-24. 2008
    ..Finally, oncogenes linked to Fah resulted in transformation of transduced hepatocytes...
  10. pmc High-level genomic integration, epigenetic changes, and expression of sleeping beauty transgene
    Jianhui Zhu
    Department of Medicine, University of Minnesota Medical School, Minneapolis, Minnesota 55455, USA
    Biochemistry 49:1507-21. 2010
    ..In addition, long-range activation of host genes must now be recognized as a potential consequence of an inserted transgene cassette containing enhancer elements...
  11. doi request reprint Stem cell origins and animal models of hepatocellular carcinoma
    Rajagopal N Aravalli
    Department of Radiology, University of Minnesota Medical School, MMC 292 Mayo, 420 Delaware Street SE, Minneapolis, MN 55455, USA
    Dig Dis Sci 55:1241-50. 2010
    ..In this review, we examine the evidence for stem cell origins of such tumors, critically evaluate existing models and reflect on how to develop new models for minimally invasive, image-guided treatment of HCC...
  12. doi request reprint Molecular mechanisms of hepatocellular carcinoma
    Rajagopal N Aravalli
    Department of Radiology, Cell Biology and Development, University of Minnesota Medical School, Minneapolis, MN, USA
    Hepatology 48:2047-63. 2008
    ..In this review, we discuss the contribution of these newer approaches toward an understanding of molecular mechanisms of HCC and for the development of novel cancer therapeutics...
  13. ncbi request reprint Administration of tauroursodeoxycholic acid (TUDCA) reduces apoptosis following myocardial infarction in rat
    Andrew L Rivard
    Department of Surgery, Experimental Surgical Services, University of Minnesota, Minneapolis, MN 55455, USA
    Am J Chin Med 35:279-95. 2007
    ..Additional studies will distinguish the functional result of improved cell survival following infarction, suggesting the potential for clinical application of this anti-apoptotic drug in treatment of acute MI...
  14. pmc Polysome trafficking of transcripts and microRNAs in regenerating liver after partial hepatectomy
    Betsy T Kren
    Dept of Medicine, Univ of Minnesota Medical School, Minneapolis, 55455, USA
    Am J Physiol Gastrointest Liver Physiol 297:G1181-92. 2009
    ..The alteration of expression for the same transcript in different polysome populations may, in part, be due to the action of miRNAs...
  15. ncbi request reprint Establishment and characterization of a unique 1 microm diameter liver-derived progenitor cell line
    Rajagopal N Aravalli
    Department of Radiology, University of Minnesota Medical School, Minneapolis, MN 55455, USA
    Biochem Biophys Res Commun 391:56-62. 2010
    ..In this report, we describe the characterization of this novel progenitor cell line that might serve as a valuable model to study liver cell functions and stem cell origin of liver cancers...
  16. ncbi request reprint Blood outgrowth endothelial cells as a vehicle for transgene expression of hepatocyte-secreted proteins via Sleeping Beauty
    Betsy T Kren
    Department of Medicine, University of Minnesota Medical School, 420 Delaware Street SE, Minneapolis, MN 55455, USA
    Endothelium 14:97-104. 2007
    ..Thus, BOECs have the potential to provide clinically relevant secreted proteins for diseases other than those of endothelial origin...
  17. pmc Nanocapsule-delivered Sleeping Beauty mediates therapeutic Factor VIII expression in liver sinusoidal endothelial cells of hemophilia A mice
    Betsy T Kren
    Department of Medicine, University of Minnesota Medical School, Minneapolis, Minnesota 55455, USA
    J Clin Invest 119:2086-99. 2009
    ..Thus, Sleeping Beauty transposon targeted to liver sinusoidal endothelial cells provided long-term expression of FVIII, without apparent antibody formation, and improved the phenotype of hemophilia A mice...
  18. ncbi request reprint Double-stranded siRNA targeted to the huntingtin gene does not induce DNA methylation
    Chang Won Park
    Department of Medicine, University of Minnesota Medical School, Minneapolis, MN 55455, USA
    Biochem Biophys Res Commun 323:275-80. 2004
    ..These results indicate that siRNA is not directly linked to DNA methylation at the target huntingtin genomic locus in human cells...
  19. ncbi request reprint Gene therapy and CVD: how near are we?
    Clifford J Steer
    Department of Medicine, University of Minnesota Medical School, Mayo Mail Code 36, Mayo Building, Room A536, 420 Delaware Street S E, Minneapolis, MN 55455, USA
    Atheroscler Suppl 5:33-42. 2004
    ..With improvements in delivery and targeting, gene therapy is likely to substantially augment established and emerging therapies in reducing the global burden of cardiovascular disease...
  20. pmc Tauroursodeoxycholic acid reduces apoptosis and protects against neurological injury after acute hemorrhagic stroke in rats
    Cecilia M P Rodrigues
    Department of Medicine, University of Minnesota Medical School, Minneapolis 55455, USA
    Proc Natl Acad Sci U S A 100:6087-92. 2003
    ..Thus, given its clinical safety, TUDCA may provide a potentially useful treatment in patients with hemorrhagic stroke and perhaps other acute brain injuries associated with cell death by apoptosis...
  21. ncbi request reprint Short, single-stranded oligonucleotides mediate targeted nucleotide conversion using extracts from isolated liver mitochondria
    Betsy T Kren
    Department of Medicine, University of Minnesota Medical School, Minneapolis, MN 55455, USA
    DNA Repair (Amst) 2:531-46. 2003
    ..The ability of unmodified SS ONs to mediate targeted alteration of the mitochondrial genome may provide a new tactic for treatment of certain mitochondrial-based diseases...
  22. doi request reprint Safety, tolerability, and cerebrospinal fluid penetration of ursodeoxycholic Acid in patients with amyotrophic lateral sclerosis
    Gareth J Parry
    Department of Neurology, University of Minnesota Medical School, Minneapolis, MN 55414, USA
    Clin Neuropharmacol 33:17-21. 2010
    ..The objective of this research was to study the safety and the tolerability of ursodeoxycholic acid in amyotrophic lateral sclerosis and document effective and dose-dependent cerebrospinal fluid penetration...
  23. doi request reprint MicroRNAs as gatekeepers of apoptosis
    Subbaya Subramanian
    Department of Laboratory Medicine and Pathology, University of Minnesota Medical School, Minneapolis, Minnesota 55455, USA
    J Cell Physiol 223:289-98. 2010
    ..Many of these apoptotic genes, irrespective of their positive or negative functional role in apoptosis, are regulated by miRNAs. In this review, we discuss the emerging role of miRNA-mediated gene networks in the control of apoptosis...
  24. ncbi request reprint Gene repair and transposon-mediated gene therapy
    Paul D Richardson
    Department of Medicine, University of Minnesota Medical School, Minneapolis, MH 55455, USA
    Stem Cells 20:105-18. 2002
    ..In addition, we review the potential application of a novel and exciting nonviral gene augmentation strategy--the Sleeping Beauty transposon system...
  25. ncbi request reprint Human DNA methyltransferase 3a does not associate with microRNAs in the regulation of DNA methylation
    Chang Won Park
    Department of Medicine, University of Minnesota Medical School, Minneapolis, MN 55455, USA
    J Cardiovasc Transl Res 3:290-5. 2010
    ..DNMT3a was not directly associated with microRNAs. But, other classes of small non-coding RNA could still be implicated with DNMTs in a specific tissue such as testis where such RNA species are highly abundant...
  26. ncbi request reprint Strategies for hepatic gene correction
    Paul Richardson
    Department of Medicine, University of Minnesota Medical School, Minneapolis 55455, USA
    J Drug Target 10:133-41. 2002
    ..In addition, we have also included a discussion of the reawakened Sleeping Beauty (SB) transposon system as a novel non-viral gene replacement strategy...
  27. ncbi request reprint Gene therapy as an alternative to liver transplantation
    Betsy T Kren
    Department of Medicine, University of Minnesota School of Medicine, Minneapolis, MN 55455, USA
    Liver Transpl 8:1089-108. 2002
    ..Although each method uses different mechanisms for gene repair and therapy, they all share a basic requirement for the efficient delivery of DNA...
  28. ncbi request reprint Oligonucleotide-mediated site-directed gene repair
    Betsy T Kren
    Department of Medicine, University of Minnesota Medical School, Minneapolis, Minnesota 55455, USA
    Methods Enzymol 346:14-35. 2002
  29. ncbi request reprint Neuroprotection by a bile acid in an acute stroke model in the rat
    Cecilia M P Rodrigues
    Department of Medicine, University of Minnesota Medical School, Minneapolis, Minnesota 55455, USA
    J Cereb Blood Flow Metab 22:463-71. 2002
    ..Thus, TUDCA, a clinically safe molecule, may be useful in the treatment of stroke and possibly other apoptosis-associated acute and chronic injuries to the brain...
  30. pmc Tauroursodeoxycholic acid, a bile acid, is neuroprotective in a transgenic animal model of Huntington's disease
    C Dirk Keene
    Graduate Program in Neuroscience and Department of Neurosurgery, University of Minnesota Medical School, Minneapolis, MN 55455, USA
    Proc Natl Acad Sci U S A 99:10671-6. 2002
    ..In conclusion, TUDCA is a nontoxic, endogenously produced hydrophilic bile acid that is neuroprotective in a transgenic mouse model of HD and, therefore, may provide a novel and effective treatment in patients with HD...
  31. pmc Site-specific base changes in the coding or promoter region of the human beta- and gamma-globin genes by single-stranded oligonucleotides
    Wenxuan Yin
    Department of Medicine, University of Minnesota Medical School, Minneapolis, MN 55455, USA
    Biochem J 390:253-61. 2005
    ..The results suggest that the endogenous DNA repair machinery may permit SSO correction of the sickle defect by modification of the beta- and/or gamma-globin genes...
  32. ncbi request reprint Sleeping Beauty transposition in the mouse genome is associated with changes in DNA methylation at the site of insertion
    Chang Won Park
    Department of Medicine, University of Minnesota Medical School, Minneapolis, MN 55455, USA
    Genomics 88:204-13. 2006
    ....
  33. ncbi request reprint Unbound E2F modulates TGF-beta1-induced apoptosis in HuH-7 cells
    Guangsheng Fan
    Department of Medicine, University of Minnesota Medical School, Minneapolis, MN 55455, USA
    J Cell Sci 115:3181-91. 2002
    ..Finally, TGF-beta1-induced apoptosis with the associated increase in E2F activity can occur in both the G(1) and S phases of the cell cycle...
  34. ncbi request reprint Long-term reduction of jaundice in Gunn rats by nonviral liver-targeted delivery of Sleeping Beauty transposon
    Xia Wang
    Department of Medicine, Marion Bessin Liver Research Center, Albert Einstein College of Medicine, Bronx 10461, NY, USA
    Hepatology 50:815-24. 2009
    ..With histidine containing FPL, serum bilirubin was reduced by 40% +/- 5%, and bilirubin glucuronides were excreted into bile. No antibodies were detectable in the recipient rats against the F-protein or human UGT1A1...
  35. ncbi request reprint The application of DNA repair vectors to gene therapy
    Betsy T Kren
    Department of Medicine, University of Minnesota Medical School MMC 36, Mayo Building A536, 420 Delaware Street, Minneapolis, MN 55455, USA
    Curr Opin Biotechnol 13:473-81. 2002
    ..Gene repair precisely targets and corrects the chromosomal mutation responsible for a genetic and/or acquired disorder. Many recent advances have been made in this area of research...
  36. pmc Heme oxygenase-1 gene delivery by Sleeping Beauty inhibits vascular stasis in a murine model of sickle cell disease
    John D Belcher
    Division of Hematology, Oncology and Transplantation, Department of Medicine, University of Minnesota, 420 Delaware St SE, MMC 480, Minneapolis, MN 55455, USA
    J Mol Med (Berl) 88:665-75. 2010
    ....
  37. ncbi request reprint Tauroursodeoxycholic acid improves the survival and function of nigral transplants in a rat model of Parkinson's disease
    Wei Ming Duan
    Department of Neurosurgery, University of Minnesota Medical School, Minneapolis 55455, USA
    Cell Transplant 11:195-205. 2002
    ..These data demonstrate that pretreatment of the cell suspension with TUDCA can reduce apoptosis and increase the survival of grafted cells, resulting in an improvement of behavioral recovery...
  38. ncbi request reprint In vivo application of non-viral vectors to the liver
    Paul Richardso
    Department of Medicine, University of Minnesota Medical School, Minneapolis 55455, USA
    J Drug Target 10:123-31. 2002
    ....
  39. ncbi request reprint DNA methylation of Sleeping Beauty with transposition into the mouse genome
    Chang Won Park
    Department of Medicine, University of Minnesota Medical School, Minneapolis, Minnesota 55455, USA
    Genes Cells 10:763-76. 2005
    ..This may depend on a number of different factors including the cargo DNA sequence, chromosomal context of the insertion site, and/or host genetic background...
  40. ncbi request reprint Gene repair in the new age of gene therapy
    Paul D Richardson
    Department of Medicine, University of Minnesota Medical School, 420 Delaware Street SE, Minneapolis, MN 55455, USA
    Hepatology 35:512-8. 2002
  41. ncbi request reprint Sleeping Beauty-mediated down-regulation of huntingtin expression by RNA interference
    Zongyu J Chen
    Department of Medicine, University of Minnesota Medical School, Minneapolis, MN 55455, USA
    Biochem Biophys Res Commun 329:646-52. 2005
    ..The combination of siRNA, the SB transposon, and an accurate transgenic mouse model may permit evaluation of this approach in preventing the pathogenesis associated with expression of mutant Htt...
  42. pmc Erythroid-specific expression of beta-globin by the sleeping beauty transposon for Sickle cell disease
    Jianhui Zhu
    Department of Medicine, University of Minnesota Medical School, Minneapolis, Minnesota 55455, USA
    Biochemistry 46:6844-58. 2007
    ..The SB-Tn system is a promising nonviral vector for efficient genomic insertion conferring stable, persistent erythroid-specific expression of beta-globin...

Research Grants6

  1. Sleeping Beauty Gene Therapy from Liver to BOECs
    Clifford Steer; Fiscal Year: 2005
    ..abstract_text> ..
  2. Sleeping Beauty Gene Therapy from Liver to BOECs
    Clifford Steer; Fiscal Year: 2006
    ..abstract_text> ..
  3. Sleeping Beauty Gene Therapy from Liver to BOECs
    Clifford Steer; Fiscal Year: 2007
    ..abstract_text> ..
  4. microRNA Uncoupling of Protein and Transcript Expression in Liver Regeneration
    Clifford Steer; Fiscal Year: 2009
    ..Ultimately, the results may provide the basis for novel therapies designed to promote liver regeneration and accelerate recovery from disease states. ..
  5. microRNA Uncoupling of Protein and Transcript Expression in Liver Regeneration
    CLIFFORD JOHN STEER; Fiscal Year: 2010
    ..Ultimately, the results may provide the basis for novel therapies designed to promote liver regeneration and accelerate recovery from disease states. ..