W Daniel Stamer

Summary

Affiliation: University of Arizona
Country: USA

Publications

  1. ncbi Isolation of primary open-angle glaucomatous trabecular meshwork cells from whole eye tissue
    D W Stamer
    Departments of Ophthalmology and Pharmacology, The University of Arizona, Tucson, Arizona 85719, USA
    Curr Eye Res 20:347-50. 2000
  2. ncbi Protein profile of exosomes from trabecular meshwork cells
    W D Stamer
    Department of Ophthalmology and Vision Science, The University of Arizona, Tucson, AZ 85711, United States
    J Proteomics 74:796-804. 2011
  3. ncbi Cellular basis for bimatoprost effects on human conventional outflow
    W Daniel Stamer
    Department of Ophthalmology and Vision Science, The University of Arizona, Tucson, Arizona 85711, USA
    Invest Ophthalmol Vis Sci 51:5176-81. 2010
  4. ncbi Sphingosine-1-phosphate effects on the inner wall of Schlemm's canal and outflow facility in perfused human eyes
    W Daniel Stamer
    Department of Ophthalmology and Vision Science, The University of Arizona, 655 North Alvernon Way, Suite 108, Tucson, AZ 85711, USA
    Exp Eye Res 89:980-8. 2009
  5. ncbi Aquaporin-1 expression and conventional aqueous outflow in human eyes
    W Daniel Stamer
    Department of Ophthalmology and Vision Science, The University of Arizona, Tucson, AZ 85711, USA
    Exp Eye Res 87:349-55. 2008
  6. ncbi Targeted gene transfer to Schlemm's canal by retroperfusion
    W Daniel Stamer
    Department of Ophthalmology, The University of Arizona, Tucson, AZ, USA
    Exp Eye Res 84:843-9. 2007
  7. ncbi Coiled-coil targeting of myocilin to intracellular membranes
    W D Stamer
    Department of Ophthalmology and Vision Science, The University of Arizona, Tucson, AZ 85711, USA
    Exp Eye Res 83:1386-95. 2006
  8. ncbi Aquaporin-1 channels in human retinal pigment epithelium: role in transepithelial water movement
    W Daniel Stamer
    Department of Ophthalmology, The University of Arizona, Tucson, Arizona 85711 1824, USA
    Invest Ophthalmol Vis Sci 44:2803-8. 2003
  9. ncbi Expression of aquaporin-1 in human trabecular meshwork cells: role in resting cell volume
    W D Stamer
    Department of Ophthalmology, The University of Arizona, 655 North Alvernon Way, Tucson, AZ 85711 1824, USA
    Invest Ophthalmol Vis Sci 42:1803-11. 2001
  10. ncbi Cannabinoid CB(1) receptor expression, activation and detection of endogenous ligand in trabecular meshwork and ciliary process tissues
    W D Stamer
    Department of Ophthalmology, The University of Arizona Health Sciences Center, Tucson, AZ 85724, USA
    Eur J Pharmacol 431:277-86. 2001

Research Grants

  1. CADHERIN DYNAMICS AND GLAUCOMA
    W Daniel Stamer; Fiscal Year: 2010
  2. CADHERIN DYNAMICS AND GLAUCOMA
    W Stamer; Fiscal Year: 2009
  3. CADHERIN DYNAMICS AND GLAUCOMA
    W Stamer; Fiscal Year: 2007
  4. SELENIUM-INDUCED GLAUCOMA
    W Stamer; Fiscal Year: 2006
  5. Molecular Basis of Myocilin Function in the Human Eye
    W Stamer; Fiscal Year: 2005
  6. CADHERIN REGULATION OF AQUEOUS HUMOR OUTFLOW
    W Stamer; Fiscal Year: 2004
  7. Molecular Basis of Myocilin Function in the Human Eye
    W Daniel Stamer; Fiscal Year: 2010

Collaborators

Detail Information

Publications38

  1. ncbi Isolation of primary open-angle glaucomatous trabecular meshwork cells from whole eye tissue
    D W Stamer
    Departments of Ophthalmology and Pharmacology, The University of Arizona, Tucson, Arizona 85719, USA
    Curr Eye Res 20:347-50. 2000
    ..The objective of this study was to directly compare the utility of two different isolation methods to obtain viable human TM cells from POAG whole eye tissue...
  2. ncbi Protein profile of exosomes from trabecular meshwork cells
    W D Stamer
    Department of Ophthalmology and Vision Science, The University of Arizona, Tucson, AZ 85711, United States
    J Proteomics 74:796-804. 2011
    ..Taken together, TM exosomes have a characteristic exosome protein profile plus contain unique proteins, including the glaucoma-causing protein, myocilin; suggesting a role for exosomes in the control of intraocular pressure...
  3. ncbi Cellular basis for bimatoprost effects on human conventional outflow
    W Daniel Stamer
    Department of Ophthalmology and Vision Science, The University of Arizona, Tucson, Arizona 85711, USA
    Invest Ophthalmol Vis Sci 51:5176-81. 2010
    ..The present study specifically examines bimatoprost effects on the cells that populate human outflow tissues...
  4. ncbi Sphingosine-1-phosphate effects on the inner wall of Schlemm's canal and outflow facility in perfused human eyes
    W Daniel Stamer
    Department of Ophthalmology and Vision Science, The University of Arizona, 655 North Alvernon Way, Suite 108, Tucson, AZ 85711, USA
    Exp Eye Res 89:980-8. 2009
    ..Regardless, S1P receptor antagonists represent novel therapeutic prospects for ocular hypertension in humans...
  5. ncbi Aquaporin-1 expression and conventional aqueous outflow in human eyes
    W Daniel Stamer
    Department of Ophthalmology and Vision Science, The University of Arizona, Tucson, AZ 85711, USA
    Exp Eye Res 87:349-55. 2008
    ..2+/-11.2%. Taken together, these results indicate that a transcellular pathway, mediated by aquaporin-1, does not contribute significantly to bulk outflow through the conventional aqueous outflow tract of human eyes...
  6. ncbi Targeted gene transfer to Schlemm's canal by retroperfusion
    W Daniel Stamer
    Department of Ophthalmology, The University of Arizona, Tucson, AZ, USA
    Exp Eye Res 84:843-9. 2007
    ....
  7. ncbi Coiled-coil targeting of myocilin to intracellular membranes
    W D Stamer
    Department of Ophthalmology and Vision Science, The University of Arizona, Tucson, AZ 85711, USA
    Exp Eye Res 83:1386-95. 2006
    ..Taken together, these data indicate that the coiled-coil domain, not the putative signal sequence, is responsible for the targeting of MYOC to the secretory machinery...
  8. ncbi Aquaporin-1 channels in human retinal pigment epithelium: role in transepithelial water movement
    W Daniel Stamer
    Department of Ophthalmology, The University of Arizona, Tucson, Arizona 85711 1824, USA
    Invest Ophthalmol Vis Sci 44:2803-8. 2003
    ....
  9. ncbi Expression of aquaporin-1 in human trabecular meshwork cells: role in resting cell volume
    W D Stamer
    Department of Ophthalmology, The University of Arizona, 655 North Alvernon Way, Tucson, AZ 85711 1824, USA
    Invest Ophthalmol Vis Sci 42:1803-11. 2001
    ..The purpose of the present study was twofold: to evaluate the prospect of manipulating AQP1 protein levels in TM cells using sense and antisense mRNA and to investigate the functional role of AQP1 in TM cells...
  10. ncbi Cannabinoid CB(1) receptor expression, activation and detection of endogenous ligand in trabecular meshwork and ciliary process tissues
    W D Stamer
    Department of Ophthalmology, The University of Arizona Health Sciences Center, Tucson, AZ 85724, USA
    Eur J Pharmacol 431:277-86. 2001
    ....
  11. ncbi Extracellular trafficking of myocilin in human trabecular meshwork cells
    Katharine M Hardy
    Department of Cell Biology, The University of Arizona, Tucson, AZ 85724, USA
    J Biol Chem 280:28917-26. 2005
    ..Taken together, data suggest that MYOC appears in the extracellular space of trabecular meshwork cells by an unconventional mechanism, likely associated with exosome-like vesicles...
  12. ncbi Bimatoprost, prostamide activity, and conventional drainage
    Zhou Wan
    Department of Ophthalmology and Vision Science, University of Arizona, Tucson, AZ 85711, USA
    Invest Ophthalmol Vis Sci 48:4107-15. 2007
    ..The purpose of the present study was to investigate the effects of bimatoprost and a novel prostamide-selective antagonist AGN 211334 on human conventional drainage...
  13. ncbi Cyclic mechanical stress and trabecular meshwork cell contractility
    Renata F Ramos
    Biomedical Engineering Graduate Program, University of Arizona, Tucson, AZ 85711, USA
    Invest Ophthalmol Vis Sci 50:3826-32. 2009
    ..The purpose of the present study was to examine responses of trabecular meshwork (TM) cells to cyclic biomechanical stress in the presence and absence of compounds known to affect cell contractility...
  14. ncbi Specific targeting of gene expression to a subset of human trabecular meshwork cells using the chitinase 3-like 1 promoter
    Paloma B Liton
    Department of Ophthalmology, Duke University, Durham, North Carolina 27710, USA
    Invest Ophthalmol Vis Sci 46:183-90. 2005
    ..This study provides a new potential tool to investigate the role of these different cell types in both normal and pathophysiological function of the outflow pathway, with implications for possible future glaucoma gene therapy...
  15. ncbi S1P₂ receptor regulation of sphingosine-1-phosphate effects on conventional outflow physiology
    Grant M Sumida
    Dept of Ophthalmology and Vision Science, University of Arizona, 655 North Alvernon Way, Tucson, AZ 85711, USA
    Am J Physiol Cell Physiol 300:C1164-71. 2011
    ..53, n = 3). These results demonstrate that S1P₂ , and not S1P₁ or S1P₃, receptor activation increases conventional outflow resistance and is a potential target to regulate intraocular pressure...
  16. ncbi Regulation of myocilin-associated exosome release from human trabecular meshwork cells
    Emely A Hoffman
    Department of Ophthalmology, University of Arizona, Tucson, Arizona 85711, USA
    Invest Ophthalmol Vis Sci 50:1313-8. 2009
    ..The goal of the present study was to determine whether the release of exosomes containing MYOC from trabecular meshwork (TM) cells is constitutive or regulated...
  17. ncbi Induction of angiogenic immediate early genes by activation of FP prostanoid receptors in cultured human ciliary smooth muscle cells
    Anthony J Hutchinson
    Program in Neuroscience, University of Arizona, Tucson, Arizona 85721, USA
    Curr Eye Res 35:408-18. 2010
    ..Herein, we describe the identification of novel signaling mechanisms that may contribute to this process...
  18. ncbi Expression analysis of the matrix GLA protein and VE-cadherin gene promoters in the outflow pathway
    Pedro Gonzalez
    Department of Ophthalmology, Duke University, Durham, North Carolina 27710, USA
    Invest Ophthalmol Vis Sci 45:1389-95. 2004
    ....
  19. ncbi Sphingosine-1-phosphate enhancement of cortical actomyosin organization in cultured human Schlemm's canal endothelial cell monolayers
    Grant M Sumida
    Physiological Sciences Graduate Interdisciplinary Program, University of Arizona, Tucson, AZ 85711, USA
    Invest Ophthalmol Vis Sci 51:6633-8. 2010
    ..Because of S1P's known effect of increasing barrier function in endothelial cells, the authors hypothesized that Schlemm's canal (SC) cells in culture respond to S1P by increasing actomyosin organization at the cell cortex...
  20. ncbi Myocilin, a component of a membrane-associated protein complex driven by a homologous Q-SNARE domain
    W Michael Dismuke
    Ophthalmology and Vision Science, University of Arizona, Tucson, Arizona 85724, United States
    Biochemistry 51:3606-13. 2012
    ..By identifying the Q-SNARE homology within the second coil of myocilin and documenting its participation in a SNARE-like complex, we provide evidence of a SNARE domain-containing protein associated with a human disease...
  21. ncbi The role of the prostaglandin EP4 receptor in the regulation of human outflow facility
    Lindsay H Millard
    Medical Pharmacology Graduate Program, University of Arizona, Tucson, Arizona, USA
    Invest Ophthalmol Vis Sci 52:3506-13. 2011
    ..Because of differences in PG efficacy in outflow function between nonhuman primates and humans, we tested the impact of 3,7-dithiaPGE(1) on conventional outflow function in human donor eyes...
  22. ncbi Human Schlemm's canal cells express the endothelial adherens proteins, VE-cadherin and PECAM-1
    Ronald L Heimark
    Department of Surgery, The University of Arizona, Tucson, AZ, USA
    Curr Eye Res 25:299-308. 2002
    ..Conclusions. VE-cadherin and PECAM-1 expression in SC suggests that SC cells are vascular in origin and contain adherens protein likely involved in restricting fluid flow across the inner wall of SC...
  23. ncbi Barriers to productive transfection of trabecular meshwork cells
    Emely A Hoffman
    Department of Ophthalmology and Vision Science, The University of Arizona, Tucson, AZ 85711, USA
    Mol Vis 11:869-75. 2005
    ..We suggest that the difficulties in transfecting cultured HTM cells may be related to the filter function of the cells in vivo where the cells must degrade exogenous DNA...
  24. ncbi eNOS, a pressure-dependent regulator of intraocular pressure
    W Daniel Stamer
    Department of Ophthalmology and Vision Science, University of Arizona, Tucson, Arizona, USA
    Invest Ophthalmol Vis Sci 52:9438-44. 2011
    ..To better understand one possible underlying molecular factor that regulates IOP, nitric oxide (NO), pressure-dependent drainage in transgenic mice overexpressing endothelial NO synthase (eNOS) was studied...
  25. ncbi Structural basement membrane components and corresponding integrins in Schlemm's canal endothelia
    Saumya S VanderWyst
    Biomedical Engineering Graduate Inter Disciplinary Program, University of Arizona, Tucson, AZ 85719, USA
    Mol Vis 17:199-209. 2011
    ..The goal of this study was to examine integrin-extracellular matrix binding partners of the inner wall basement membrane that facilitate attachment...
  26. ncbi L-DOPA is an endogenous ligand for OA1
    Vanessa M Lopez
    Department of Ophthalmology and Vision Science, The University of Arizona, Tucson, Arizona, USA
    PLoS Biol 6:e236. 2008
    ..Our results suggest that the retinal consequences of albinism caused by changes in melanin synthetic machinery may be treated by L-DOPA supplementation...
  27. ncbi Alpha-2 adrenergic receptors stimulate actin organization in developing fetal rat cardiac myocytes
    Amy C Porter
    Department of Pharmacology and Toxicology, College of Pharmacy, The University of Arizona, Tucson, AZ 85721, USA
    Life Sci 72:1455-66. 2003
    ..Therefore, functional alpha(2)A and alpha(2)B subtypes are present in the fetal rat heart where they may have a role in cardiac development...
  28. ncbi Enhanced inflow and outflow rates despite lower IOP in bestrophin-2-deficient mice
    Youwen Zhang
    Department of Ophthalmology and Vision Science, University of Arizona, Tucson, Arizona 85711, USA
    Invest Ophthalmol Vis Sci 50:765-70. 2009
    ..Disruption of Best2 in mice results in a diminished intraocular pressure (IOP). Aqueous humor dynamics were compared in Best2(+/+) and Best2(-/-) mice, to better understand the contribution of Best2 to IOP...
  29. ncbi Effects of cyclic intraocular pressure on conventional outflow facility
    Renata F Ramos
    Biomedical Engineering Graduate Program, University of Arizona, Tucson, AZ 85711, USA
    Invest Ophthalmol Vis Sci 49:275-81. 2008
    ..The purpose of the present study was to determine the effect(s) of ocular pulse on conventional outflow facility in perfused anterior segments...
  30. ncbi Tetraethylammonium block of water flux in Aquaporin-1 channels expressed in kidney thin limbs of Henle's loop and a kidney-derived cell line
    Andrea J Yool
    Dept of Physiology, University of Arizona College of Medicine, Tucson, Arizona, USA
    BMC Physiol 2:4. 2002
    ..We also demonstrate that TEA does not inhibit the cGMP-dependent ionic conductance of AQP1 expressed in oocytes, supporting the idea that water and ion fluxes involve pharmacologically distinct pathways in the AQP1 tetrameric complex...
  31. ncbi Alterations in human trabecular meshwork cell homeostasis by selenium
    Shannon M Conley
    Department of Pharmacology and Ophthalmology, University of Arizona, 655 North Alvernon Way, Suite 108, Tucson, AZ 85711, USA
    Exp Eye Res 82:637-47. 2006
    ..Similar results with HUVECs have implications for understanding selenium's mechanisms of action as an anti-angiogenic agent...
  32. ncbi Stimulation of aquaporin-mediated fluid transport by cyclic GMP in human retinal pigment epithelium in vitro
    Nicholas W Baetz
    Department of Cell Biology and Anatomy, University of Arizona, Tucson, Arizona, USA
    Invest Ophthalmol Vis Sci 53:2127-32. 2012
    ..We hypothesized that stimulation of the natriuretic signaling pathway in RPE with atrial natriuretic peptide (ANP) and with membrane-permeable analogs of cGMP would induce a net apical-to-basal transport of fluid...
  33. ncbi Selenium's effects on MMP-2 and TIMP-1 secretion by human trabecular meshwork cells
    Shannon M Conley
    Department of Ophthalmology, University of Arizona, Tucson, Arizona 85711, USA
    Invest Ophthalmol Vis Sci 45:473-9. 2004
    ....
  34. ncbi Ion channel function of aquaporin-1 natively expressed in choroid plexus
    Daniela Boassa
    Program in Neuroscience, Department of Ophthalmology and Vision Science, University of Arizona, Tucson, Arizona 85724, USA
    J Neurosci 26:7811-9. 2006
    ..The conclusion that the AQP1-associated cation current contributes to modulating CSF production resolves a lingering concern as to whether an aquaporin ionic conductance can have a physiologically relevant function...
  35. ncbi Expression of alpha(2)-adrenergic receptor subtypes in prenatal rat spinal cord
    Yi Huang
    Department of Physiology, College of Medicine, University of Arizona, Tucson, AZ 85721, USA
    Brain Res Dev Brain Res 133:93-104. 2002
    ....
  36. ncbi Human trabecular meshwork cell volume regulation
    Claire H Mitchell
    Department of Physiology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104-6085, USA
    Am J Physiol Cell Physiol 283:C315-26. 2002
    ..Results indicate that human TM cells display a Ca2+-independent RVD and that volume is regulated by swelling-activated K+ and Cl- channels, Na+/H+ antiports, and possibly K+-Cl- symports in addition to Na+-K+-2Cl- symports...
  37. ncbi Differential P1-purinergic modulation of human Schlemm's canal inner-wall cells
    Mike O Karl
    Dept. of Physiology, School of Medicine, A303 Richards Bldg, Univ. of Pennsylvania, Philadelphia, PA 19104-6085, USA
    Am J Physiol Cell Physiol 288:C784-94. 2005
    ..The results are also consistent with the presence of functional A2B, as well as A1, A2A, and A3 adenosine receptors in SC cells...
  38. ncbi Experimental and numerical studies of adenovirus delivery to outflow tissues of perfused human anterior segments
    C Ross Ethier
    Department of Mechanical and Industrial Engineering, University of Toronto, Toronto, Ontario, Canada
    Invest Ophthalmol Vis Sci 45:1863-70. 2004
    ..To investigate the efficacy of two different methods of adenoviral transfer of genes to trabecular meshwork (TM) and Schlemm's canal (SC) cells in cultured human anterior segments, using both experimental and numerical analyses...

Research Grants14

  1. CADHERIN DYNAMICS AND GLAUCOMA
    W Daniel Stamer; Fiscal Year: 2010
    ..Results obtained from these investigations will provide a basic understanding of the role of cadherin proteins in aqueous outflow resistance and uncover novel therapeutic targets for glaucoma therapy. ..
  2. CADHERIN DYNAMICS AND GLAUCOMA
    W Stamer; Fiscal Year: 2009
    ..Results obtained from these investigations will provide a basic understanding of the role of cadherin proteins in aqueous outflow resistance and uncover novel therapeutic targets for glaucoma therapy. ..
  3. CADHERIN DYNAMICS AND GLAUCOMA
    W Stamer; Fiscal Year: 2007
    ..Results obtained from these investigations will provide a basic understanding of the role of cadherin proteins in aqueous outflow resistance and uncover novel therapeutic targets for glaucoma therapy. ..
  4. SELENIUM-INDUCED GLAUCOMA
    W Stamer; Fiscal Year: 2006
    ..abstract_text> ..
  5. Molecular Basis of Myocilin Function in the Human Eye
    W Stamer; Fiscal Year: 2005
    ..This knowledge will contribute to our understanding of glaucoma at the molecular level and provide new therapeutic targets for the treatment of those who are afflicted with glaucoma. ..
  6. CADHERIN REGULATION OF AQUEOUS HUMOR OUTFLOW
    W Stamer; Fiscal Year: 2004
    ..abstract_text> ..
  7. Molecular Basis of Myocilin Function in the Human Eye
    W Daniel Stamer; Fiscal Year: 2010
    ..abstract_text> ..