Deepak Srivastava

Summary

Affiliation: University of California
Country: USA

Publications

  1. pmc Cardiac reprogramming: from mouse toward man
    Deepak Srivastava
    Gladstone Institute of Cardiovascular Disease, San Francisco, CA 94158, USA Roddenberry Center for Stem Cell Biology and Medicine, San Francisco, CA 94158, USA Department of Pediatrics, University of California, San Francisco, CA 94158, USA Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94158, USA Electronic address
    Curr Opin Genet Dev 23:574-8. 2013
  2. pmc A role for RNA post-transcriptional regulation in satellite cell activation
    Nicholas H Farina
    Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO, 80309, USA
    Skelet Muscle 2:21. 2012
  3. doi request reprint Cardiac repair with thymosin β4 and cardiac reprogramming factors
    Deepak Srivastava
    Gladstone Institute of Cardiovascular Disease, San Francisco, California 94158, USA
    Ann N Y Acad Sci 1270:66-72. 2012
  4. ncbi request reprint Potential of stem-cell-based therapies for heart disease
    Deepak Srivastava
    Gladstone Institute of Cardiovascular Disease and Department of Pediatrics, University of California San Francisco, 1650 Owens Street, San Francisco, California 94158, USA
    Nature 441:1097-9. 2006
  5. ncbi request reprint Genetic regulation of cardiogenesis and congenital heart disease
    Deepak Srivastava
    Gladstone Institute of Cardiovascular Disease, Department of Pediatrics, University of California, San Francisco, California 94158, USA
    Annu Rev Pathol 1:199-213. 2006
  6. ncbi request reprint Thymosin beta4 is cardioprotective after myocardial infarction
    Deepak Srivastava
    Gladstone Institute of Cardiovascular Disease, 1650 Owens Street, San Francisco, CA 94158, USA
    Ann N Y Acad Sci 1112:161-70. 2007
  7. ncbi request reprint Making or breaking the heart: from lineage determination to morphogenesis
    Deepak Srivastava
    Gladstone Institute of Cardiovascular Disease and Department of Pediatrics and Biochemistry, University of California, San Francisco, CA 94158, USA
    Cell 126:1037-48. 2006
  8. pmc A regulatory pathway involving Notch1/beta-catenin/Isl1 determines cardiac progenitor cell fate
    Chulan Kwon
    Gladstone Institute of Cardiovascular Disease and Departments of Pediatrics and Biochemistry and Biophysics, University of California, San Francisco, 1650 Owens Street, San Francisco, CA 94158, USA
    Nat Cell Biol 11:951-7. 2009
  9. pmc Interaction of Gata4 and Gata6 with Tbx5 is critical for normal cardiac development
    Meenakshi Maitra
    Department of Pediatrics, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    Dev Biol 326:368-77. 2009
  10. pmc The neural crest-enriched microRNA miR-452 regulates epithelial-mesenchymal signaling in the first pharyngeal arch
    Neil T Sheehy
    Gladstone Institute of Cardiovascular Disease, San Francisco, CA 94158, USA
    Development 137:4307-16. 2010

Research Grants

  1. Cardiogenesis: Molecular Mechanisms
    Deepak Srivastava; Fiscal Year: 2010
  2. Isolated and Syndromic Cardiac Outflow Tract Defects
    Deepak Srivastava; Fiscal Year: 2006
  3. CARDIOGENESIS-- MOLECULAR MECHANISMS
    Deepak Srivastava; Fiscal Year: 2005
  4. Cardiogenesis: Molecular Mechanisms
    Deepak Srivastava; Fiscal Year: 2007
  5. Cardiac Defects/Cross-species analysis/Human Mutations
    Deepak Srivastava; Fiscal Year: 2009
  6. Cardiogenesis: Molecular Mechanisms
    Deepak Srivastava; Fiscal Year: 2009
  7. CARDIOGENESIS--MOLECULAR MECHANISMS
    Deepak Srivastava; Fiscal Year: 2000
  8. SYNDROMIC CARDIAC OUTFLOW TRACT DEFECTS
    Deepak Srivastava; Fiscal Year: 2002
  9. Cardiac Development and Cogenital Heart Disease
    Deepak Srivastava; Fiscal Year: 2004

Detail Information

Publications84

  1. pmc Cardiac reprogramming: from mouse toward man
    Deepak Srivastava
    Gladstone Institute of Cardiovascular Disease, San Francisco, CA 94158, USA Roddenberry Center for Stem Cell Biology and Medicine, San Francisco, CA 94158, USA Department of Pediatrics, University of California, San Francisco, CA 94158, USA Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94158, USA Electronic address
    Curr Opin Genet Dev 23:574-8. 2013
    ..Here we discuss the advances in direct cardiac reprogramming that will potentially act as a springboard in the generation of effective approaches to restoring cardiac function after injury. ..
  2. pmc A role for RNA post-transcriptional regulation in satellite cell activation
    Nicholas H Farina
    Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO, 80309, USA
    Skelet Muscle 2:21. 2012
    ..abstract:..
  3. doi request reprint Cardiac repair with thymosin β4 and cardiac reprogramming factors
    Deepak Srivastava
    Gladstone Institute of Cardiovascular Disease, San Francisco, California 94158, USA
    Ann N Y Acad Sci 1270:66-72. 2012
    ..These findings suggest that thymosin β4 and cardiac reprogramming technology may synergistically limit damage to the heart and promote cardiac regeneration through the stimulation of endogenous cells within the heart...
  4. ncbi request reprint Potential of stem-cell-based therapies for heart disease
    Deepak Srivastava
    Gladstone Institute of Cardiovascular Disease and Department of Pediatrics, University of California San Francisco, 1650 Owens Street, San Francisco, California 94158, USA
    Nature 441:1097-9. 2006
    ..The exciting discoveries in basic science will require rigorous testing in animal models to determine those most worthy of future clinical trials...
  5. ncbi request reprint Genetic regulation of cardiogenesis and congenital heart disease
    Deepak Srivastava
    Gladstone Institute of Cardiovascular Disease, Department of Pediatrics, University of California, San Francisco, California 94158, USA
    Annu Rev Pathol 1:199-213. 2006
    ..The lessons learned from examining the early steps of heart formation are essential for informing the prevention of malformations and their long-term consequences, as well as for approaches to guide stem cells into cardiac lineages...
  6. ncbi request reprint Thymosin beta4 is cardioprotective after myocardial infarction
    Deepak Srivastava
    Gladstone Institute of Cardiovascular Disease, 1650 Owens Street, San Francisco, CA 94158, USA
    Ann N Y Acad Sci 1112:161-70. 2007
    ..These findings suggest that thymosin beta4 promotes cardiomyocyte and endothelial migration, survival, and repair and may be a novel therapeutic target in the setting of acute myocardial damage...
  7. ncbi request reprint Making or breaking the heart: from lineage determination to morphogenesis
    Deepak Srivastava
    Gladstone Institute of Cardiovascular Disease and Department of Pediatrics and Biochemistry, University of California, San Francisco, CA 94158, USA
    Cell 126:1037-48. 2006
    ....
  8. pmc A regulatory pathway involving Notch1/beta-catenin/Isl1 determines cardiac progenitor cell fate
    Chulan Kwon
    Gladstone Institute of Cardiovascular Disease and Departments of Pediatrics and Biochemistry and Biophysics, University of California, San Francisco, 1650 Owens Street, San Francisco, CA 94158, USA
    Nat Cell Biol 11:951-7. 2009
    ..These findings reveal a regulatory network controlling CPC expansion and cell fate that involves unanticipated functions of beta-catenin, Notch1 and Isl1 that may be leveraged for regenerative approaches involving CPCs...
  9. pmc Interaction of Gata4 and Gata6 with Tbx5 is critical for normal cardiac development
    Meenakshi Maitra
    Department of Pediatrics, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    Dev Biol 326:368-77. 2009
    ..These findings highlight the unique genetic interactions of Gata4 and Gata6 with Tbx5 for normal cardiac morphogenesis in vivo...
  10. pmc The neural crest-enriched microRNA miR-452 regulates epithelial-mesenchymal signaling in the first pharyngeal arch
    Neil T Sheehy
    Gladstone Institute of Cardiovascular Disease, San Francisco, CA 94158, USA
    Development 137:4307-16. 2010
    ..These results suggest that post-transcriptional regulation by miRNAs is required for differentiation of NCC-derived tissues and that miR-452 is involved in epithelial-mesenchymal signaling in the pharyngeal arch...
  11. pmc skNAC, a Smyd1-interacting transcription factor, is involved in cardiac development and skeletal muscle growth and regeneration
    Chong Yon Park
    Department of Pediatrics, Gladstone Institute of Cardiovascular Disease, University of California, San Francisco, CA 94158, USA
    Proc Natl Acad Sci U S A 107:20750-5. 2010
    ..Our results indicate that skNAC plays a critical role in ventricular cardiomyocyte expansion and regulates postnatal skeletal muscle growth and regeneration in mice...
  12. pmc miR-145 and miR-143 regulate smooth muscle cell fate and plasticity
    Kimberly R Cordes
    Gladstone Institute of Cardiovascular Disease, San Francisco, California 94158, USA
    Nature 460:705-10. 2009
    ..These findings demonstrate that miR-145 can direct the smooth muscle fate and that miR-145 and miR-143 function to regulate the quiescent versus proliferative phenotype of smooth muscle cells...
  13. pmc Direct reprogramming of fibroblasts into functional cardiomyocytes by defined factors
    Masaki Ieda
    Gladstone Institute of Cardiovascular Disease, University of California, San Francisco, San Francisco, CA 94158, USA
    Cell 142:375-86. 2010
    ..Reprogramming of endogenous or explanted fibroblasts might provide a source of cardiomyocytes for regenerative approaches...
  14. ncbi request reprint Tbx1 regulates fibroblast growth factors in the anterior heart field through a reinforcing autoregulatory loop involving forkhead transcription factors
    Tonghuan Hu
    Department of Pediatrics, University of Texas Southwestern Medical Center, Dallas, TX 75390 9148, USA
    Development 131:5491-502. 2004
    ....
  15. pmc Notch post-translationally regulates β-catenin protein in stem and progenitor cells
    Chulan Kwon
    Gladstone Institute of Cardiovascular Disease and Department of Pediatrics, University of California, San Francisco, 1650 Owens Street, San Francisco, California 94158, USA
    Nat Cell Biol 13:1244-51. 2011
    ..It did, however, require the endocytic adaptor protein Numb and lysosomal activity. This study reveals a previously unrecognized function of Notch in negatively titrating active β-catenin protein levels in stem and progenitor cells...
  16. pmc In vivo reprogramming of murine cardiac fibroblasts into induced cardiomyocytes
    Li Qian
    1Gladstone Institute of Cardiovascular Disease, San Francisco, California 94158, USA
    Nature 485:593-8. 2012
    ..These findings demonstrate that cardiac fibroblasts can be reprogrammed into cardiomyocyte-like cells in their native environment for potential regenerative purposes...
  17. pmc Reporter-based isolation of induced pluripotent stem cell- and embryonic stem cell-derived cardiac progenitors reveals limited gene expression variance
    Linda W Van Laake
    Gladstone Institute of Cardiovascular Disease, University of California, San Francisco, 94158, USA
    Circ Res 107:340-7. 2010
    ....
  18. pmc Hand2 function in second heart field progenitors is essential for cardiogenesis
    Takatoshi Tsuchihashi
    Gladstone Institute of Cardiovascular Disease, University of California San Francisco, 1650 Owens Street, San Francisco, CA 94158, USA
    Dev Biol 351:62-9. 2011
    ..These findings demonstrate that Hand2 is essential for survival of second heart field progenitors and that the graded loss of Hand2 function in this cardiac progenitor pool can cause a spectrum of congenital heart malformation...
  19. pmc Cardiac fibroblasts regulate myocardial proliferation through beta1 integrin signaling
    Masaki Ieda
    Gladstone Institute of Cardiovascular Disease, University of California, San Francisco, 94158, USA
    Dev Cell 16:233-44. 2009
    ..These findings reveal a previously unrecognized paracrine function of embryonic cardiac fibroblasts in regulating cardiomyocyte proliferation...
  20. ncbi request reprint Thymosin beta4 activates integrin-linked kinase and promotes cardiac cell migration, survival and cardiac repair
    Ildiko Bock-Marquette
    Department of Pediatrics, University of Texas Southwestern Medical Center, 6000 Harry Hines Blvd, Dallas, Texas 75390 9148, USA
    Nature 432:466-72. 2004
    ..These findings suggest that thymosin beta4 promotes cardiomyocyte migration, survival and repair and the pathway it regulates may be a new therapeutic target in the setting of acute myocardial damage...
  21. pmc miR-126 regulates angiogenic signaling and vascular integrity
    Jason E Fish
    Gladstone Institute of Cardiovascular Disease, San Francisco, CA 94158, USA
    Dev Cell 15:272-84. 2008
    ..These findings illustrate that a single miRNA can regulate vascular integrity and angiogenesis, providing a new target for modulating vascular formation and function...
  22. pmc microRNA-138 modulates cardiac patterning during embryonic development
    Sarah U Morton
    Gladstone Institute of Cardiovascular Disease, Department of Pediatrics, University of California, San Francisco, CA 94158, USA
    Proc Natl Acad Sci U S A 105:17830-5. 2008
    ....
  23. ncbi request reprint Dysregulation of cardiogenesis, cardiac conduction, and cell cycle in mice lacking miRNA-1-2
    Yong Zhao
    Gladstone Institute of Cardiovascular Disease, 1650 Owens Street, San Francisco, CA 94158, USA
    Cell 129:303-17. 2007
    ..These findings indicate that subtle alteration of miRNA dosage can have profound consequences in mammals and demonstrate the utility of mammalian loss-of-function models in revealing physiologic miRNA targets...
  24. pmc A Slit/miR-218/Robo regulatory loop is required during heart tube formation in zebrafish
    Jason E Fish
    Gladstone Institute of Cardiovascular Disease, San Francisco, CA 94158, USA
    Development 138:1409-19. 2011
    ..These findings suggest a new paradigm for microRNA-based control of ligand-receptor interactions and provide evidence for a novel signaling pathway regulating vertebrate heart tube assembly...
  25. pmc Fibronectin mediates mesendodermal cell fate decisions
    Paul Cheng
    Gladstone Institute of Cardiovascular Disease, San Francisco, CA 94158, USA
    Development 140:2587-96. 2013
    ....
  26. pmc MicroRNA-10 regulates the angiogenic behavior of zebrafish and human endothelial cells by promoting vascular endothelial growth factor signaling
    David Hassel
    Gladstone Institute of Cardiovascular Disease, San Francisco, CA 94158, USA
    Circ Res 111:1421-33. 2012
    ..Various signaling pathways control the behavior of endothelial cells, but their posttranscriptional dose titration by microRNAs is poorly understood...
  27. pmc Stromal cell-derived factor-1alpha is cardioprotective after myocardial infarction
    Ankur Saxena
    Gladstone Institute of Cardiovascular Disease, 1650 Owens St, San Francisco, CA 94158, USA
    Circulation 117:2224-31. 2008
    ..One possible cell-independent alternative is the direct administration of small proteins to damaged myocardium...
  28. pmc MicroRNA regulation of cell lineages in mouse and human embryonic stem cells
    Kathryn N Ivey
    Gladstone Institute of Cardiovascular Disease, University of California, San Francisco, 1650 Owens Street, San Francisco, CA 94158, USA
    Cell Stem Cell 2:219-29. 2008
    ..Our findings indicate that muscle-specific miRNAs reinforce the silencing of nonmuscle genes during cell lineage commitment and suggest that miRNAs may have general utility in regulating cell-fate decisions from pluripotent ES cells...
  29. ncbi request reprint Mutations in NOTCH1 cause aortic valve disease
    Vidu Garg
    Department of Pediatrics, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9148, USA
    Nature 437:270-4. 2005
    ..These results suggest that NOTCH1 mutations cause an early developmental defect in the aortic valve and a later de-repression of calcium deposition that causes progressive aortic valve disease...
  30. pmc A rare human sequence variant reveals myocardin autoinhibition
    Joshua F Ransom
    Gladstone Institute of Cardiovascular Disease, University of California, San Francisco, California 94158, USA
    J Biol Chem 283:35845-52. 2008
    ..These findings identify a novel mechanism that regulates levels of MYOCD-dependent activation of the SRF genetic program differentially in cardiac and smooth muscle...
  31. pmc miR-24 inhibits apoptosis and represses Bim in mouse cardiomyocytes
    Li Qian
    Gladstone Institute of Cardiovascular Disease, University of California, San Francisco, San Francisco, CA 94143, USA
    J Exp Med 208:549-60. 2011
    ..This antiapoptotic effect on cardiomyocytes in vivo was partially mediated by Bim. Our results suggest that manipulating miRNA levels during stress-induced apoptosis may be a novel therapeutic strategy for cardiac disease...
  32. pmc Limited gene expression variation in human embryonic stem cell and induced pluripotent stem cell-derived endothelial cells
    Mark P White
    Gladstone Institute of Cardiovascular Disease, San Francisco, California 94158, USA
    Stem Cells 31:92-103. 2013
    ....
  33. ncbi request reprint Loss of Apaf-1 leads to partial rescue of the HAND2-null phenotype
    Aparna R Aiyer
    Department of Pediatrics, University of Texas Southwestern Medical Center at Dallas, 6000 Harry Hines Boulevard, Room NA8 124, Dallas, TX 75390 9148, USA
    Dev Biol 278:155-62. 2005
    ....
  34. pmc A genome-wide screen reveals a role for microRNA-1 in modulating cardiac cell polarity
    Isabelle N King
    Gladstone Institute of Cardiovascular Disease, San Francisco, CA 94158, USA
    Dev Cell 20:497-510. 2011
    ..These findings illustrate the power of Drosophila-based screens to find points of intersection between miRNAs and conserved pathways in mammals...
  35. ncbi request reprint Altered microRNAs in bicuspid aortic valve: a comparison between stenotic and insufficient valves
    Vishal Nigam
    Department of Pediatrics Cardiology, University of California, San Francisco, USA
    J Heart Valve Dis 19:459-65. 2010
    ..In-vitro experiments were also carried out to determine if these miRNAs could modulate calcification-related genes...
  36. doi request reprint Reprogramming of mouse fibroblasts into cardiomyocyte-like cells in vitro
    Li Qian
    Gladstone Institute of Cardiovascular Disease, San Francisco, CA, USA
    Nat Protoc 8:1204-15. 2013
    ..Here we describe a detailed step-by-step protocol for in vitro cardiac reprogramming using retroviruses encoding GMT...
  37. pmc Spatiotemporal regulation of an Hcn4 enhancer defines a role for Mef2c and HDACs in cardiac electrical patterning
    Vasanth Vedantham
    Gladstone Institute of Cardiovascular Disease, University of California, San Francisco, CA 94158, USA
    Dev Biol 373:149-62. 2013
    ..These findings reveal mechanisms that control the distribution of automaticity among cardiomyocytes during development and in response to stress...
  38. pmc MicroRNA regulation of cardiovascular development
    Kimberly R Cordes
    Gladstone Institute of Cardiovascular Disease, 1650 Owens St, San Francisco, CA 94158, USA
    Circ Res 104:724-32. 2009
    ..Further analysis of miRNA function during cardiovascular development will allow us to determine the potential for novel miRNA-based therapeutic strategies...
  39. pmc AKAP13 Rho-GEF and PKD-binding domain deficient mice develop normally but have an abnormal response to β-adrenergic-induced cardiac hypertrophy
    Matthew J Spindler
    Gladstone Institute of Cardiovascular Disease, San Francisco, California, USA
    PLoS ONE 8:e62705. 2013
    ..Additionally, the AKAP13 Rho-GEF and PKD-binding domains mediate cardiomyocyte hypertrophy in cell culture. However, the requirements for the Rho-GEF and PKD-binding domains during development and cardiac hypertrophy are unknown...
  40. pmc MicroRNAs in cardiac development
    Kimberly R Cordes
    Gladstone Institute of Cardiovascular Disease and Department of Pediatrics, University of California, San Francisco, CA 94158, USA
    Pediatr Cardiol 31:349-56. 2010
    ..Further analysis of miRNA function during cardiovascular development will allow us to determine the potential for novel miRNA-based therapeutic strategies...
  41. pmc Identification of GATA6 sequence variants in patients with congenital heart defects
    Meenakshi Maitra
    Department of Pediatrics, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    Pediatr Res 68:281-5. 2010
    ..These data suggest that nonsynonymous GATA6 sequence variants are infrequently found in individuals with CHD...
  42. pmc Monkeying around with cardiac progenitors: hope for the future
    Li Qian
    Gladstone Institute of Cardiovascular Disease, Department of Pediatrics, University of California, San Francisco, California 94158, USA
    J Clin Invest 120:1034-6. 2010
    ..These findings move the field another step closer to clinical use of ES or iPS cell-derived cardiovascular progenitors in cardiac repair...
  43. pmc Notch1 represses osteogenic pathways in aortic valve cells
    Vishal Nigam
    Gladstone Institute of Cardiovascular Disease and Departments of Pediatrics, University of California, San Francisco, CA 94158, USA
    J Mol Cell Cardiol 47:828-34. 2009
    ..siRNA-mediated knockdown of Bmp2 blocked the calcification induced by Notch inhibition in AVICs. These findings suggest that Notch1 signaling in aortic valve cells represses osteoblast-like calcification pathways mediated by Bmp2...
  44. pmc Transcriptional regulation during development of the ductus arteriosus
    Kathryn N Ivey
    Department of Pediatrics, University of Texas Southwestern Medical Center, Dallas, USA
    Circ Res 103:388-95. 2008
    ....
  45. pmc MicroRNA1 influences cardiac differentiation in Drosophila and regulates Notch signaling
    Chulan Kwon
    Gladstone Institute of Cardiovascular Disease and Department of Pediatrics, University of California, 1650 Owens Street, San Francisco, CA 94158
    Proc Natl Acad Sci U S A 102:18986-91. 2005
    ..These findings demonstrate that dmiR-1 may "fine-tune" critical steps involved in differentiation of cardiac and somatic muscle progenitors and targets a pathway required for progenitor cell specification and asymmetric cell division...
  46. pmc The chemokine receptor CXCR7 functions to regulate cardiac valve remodeling
    Sangho Yu
    Gladstone Institute of Cardiovascular Disease, San Francisco, California, USA
    Dev Dyn 240:384-93. 2011
    ..Thus, CXCR7 is involved in semilunar valve development, possibly by regulating BMP signaling, and may contribute to aortic and pulmonary valve stenosis...
  47. pmc Canonical Wnt signaling is a positive regulator of mammalian cardiac progenitors
    Chulan Kwon
    Gladstone Institute of Cardiovascular Disease and Department of Pediatrics, University of California, 1650 Owens Street, San Francisco, CA 94158, USA
    Proc Natl Acad Sci U S A 104:10894-9. 2007
    ..Together, these data provide in vivo and in vitro evidence that canonical Wnt signaling promotes the expansion of cardiac progenitors and differentiation of cardiomyocytes...
  48. ncbi request reprint Hrt and Hes negatively regulate Notch signaling through interactions with RBP-Jkappa
    Isabelle N King
    Department of Pediatrics, The University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390 9063, USA
    Biochem Biophys Res Commun 345:446-52. 2006
    ..These findings suggest a novel mechanism for negative feedback on Notch signaling that requires RBP-Jkappa to interact physically with Hrt and Hes...
  49. doi request reprint Stem cell therapy for cardiac disease
    Harold S Bernstein
    Department of Pediatrics, University of California, San Francisco, San Francisco, California, USA
    Pediatr Res 71:491-9. 2012
    ..Finally, we will review progress being made in assessing functional improvement in animals and humans after cellular transplant...
  50. pmc Elevated miR-499 levels blunt the cardiac stress response
    Joseph T C Shieh
    Gladstone Institute of Cardiovascular Disease, San Francisco, California, United States of America
    PLoS ONE 6:e19481. 2011
    ..Here we investigate miR-499, a microRNA embedded within a ventricular-specific myosin heavy chain gene, which is expressed in heart and skeletal muscle...
  51. ncbi request reprint Building a heart: implications for congenital heart disease
    Deepak Srivastava
    Department of Pediatrics, University of Texas Southwestern Medical Center, Dallas, Tex 75390 9148, USA
    J Nucl Cardiol 10:63-70. 2003
  52. doi request reprint MicroRNAs as regulators of differentiation and cell fate decisions
    Kathryn N Ivey
    Department of Pediatrics, Gladstone Institute of Cardiovascular Disease, University of California San Francisco, 1650 Owens Street, San Francisco, CA 94158, USA
    Cell Stem Cell 7:36-41. 2010
    ..We discuss this emerging topic, in part using muscle differentiation as a paradigm, and highlight common themes and unique modalities by which microRNAs exert their lineage-promoting or differentiation effects on multiple tissues...
  53. ncbi request reprint Screening and biochemical analysis of GATA4 sequence variations identified in patients with congenital heart disease
    Marie K Schluterman
    Department of Pediatrics, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9148, USA
    Am J Med Genet A 143:817-23. 2007
    ....
  54. pmc Wnt/beta-catenin signaling acts at multiple developmental stages to promote mammalian cardiogenesis
    Chulan Kwon
    Gladstone Institute of Cardiovascular Disease, San Francisco, San Francisco, California 94158, USA
    Cell Cycle 7:3815-8. 2008
    ..Here we discuss the required roles of Wnt/beta-catenin signaling at the different stages of heart development...
  55. doi request reprint The let-7/LIN-41 pathway regulates reprogramming to human induced pluripotent stem cells by controlling expression of prodifferentiation genes
    Kathleen A Worringer
    Gladstone Institute of Cardiovascular Disease, San Francisco, CA 94158, USA Roddenberry Center for Stem Cell Biology and Medicine at Gladstone, San Francisco, CA 94158, USA
    Cell Stem Cell 14:40-52. 2014
    ..Together our findings outline a let-7-based pathway that counteracts the activity of reprogramming factors through promoting the expression of prodifferentiation genes...
  56. pmc A resource for the conditional ablation of microRNAs in the mouse
    Chong Yon Park
    UCSF Diabetes Center, UCSF, San Francisco, CA 94143, USA
    Cell Rep 1:385-91. 2012
    ..These data and collection of resources will be of value for the in vivo dissection of miRNA functions in mouse models...
  57. ncbi request reprint GATA4 mutations cause human congenital heart defects and reveal an interaction with TBX5
    Vidu Garg
    Department of Pediatrics, University of Texas Southwestern Medical Center at Dallas, 6000 Harry Hines Boulevard, Rm NA8 124, Dallas, Texas 75390 9148, USA
    Nature 424:443-7. 2003
    ..These results implicate GATA4 as a genetic cause of human cardiac septal defects, perhaps through its interaction with TBX5...
  58. ncbi request reprint Functional attenuation of UFD1l, a 22q11.2 deletion syndrome candidate gene, leads to cardiac outflow septation defects in chicken embryos
    Chihiro Yamagishi
    Department of Pediatrics, University of Texas, Southwestern Medical Center at Dallas, Dallas 75390 9148, USA
    Pediatr Res 53:546-53. 2003
    ..These data suggest that Ufd1l may play a role in cardiac NCC during conotruncal septation...
  59. pmc MicroRNAs: opening a new vein in angiogenesis research
    Jason E Fish
    Gladstone Institute of Cardiovascular Disease, 1650 Owens Street, San Francisco, CA 94158, USA
    Sci Signal 2:pe1. 2009
    ..Additional microRNAs have been implicated in the regulation of various aspects of angiogenesis. Thus, targeting the expression of microRNAs may be a novel therapeutic approach for diseases involving excess or insufficient vasculature...
  60. ncbi request reprint Stretching to meet needs: integrin-linked kinase and the cardiac pump
    Deepak Srivastava
    Gladstone Institute of Cardiovascular Disease and Department of Pediatrics, University of California at San Francisco, San Francisco, California 94158, USA
    Genes Dev 20:2327-31. 2006
  61. ncbi request reprint A developmental view of microRNA function
    Yong Zhao
    Gladstone Institute of Cardiovascular Disease, University of California, San Francisco, 1650 Owens Street, San Francisco, CA 94158, USA
    Trends Biochem Sci 32:189-97. 2007
    ..Future discoveries in this area are likely to reveal developmental-regulation and disease mechanisms related to miRNAs...
  62. pmc Derivation conditions impact X-inactivation status in female human induced pluripotent stem cells
    Kiichiro Tomoda
    Gladstone Institute of Cardiovascular Disease, University of California, San Francisco, San Francisco, CA 94158, USA
    Cell Stem Cell 11:91-9. 2012
    ..Thus, feeders are a significant factor affecting X-inactivation status. The efficient production of Xa/Xa hiPSC lines provides unprecedented opportunities to understand human X-reactivation and -inactivation...
  63. ncbi request reprint The genetics of cardiac birth defects
    Joshua Ransom
    Gladstone Institute of Cardiovascular Disease and the Department of Pediatrics, University of California at San Francisco, 1650 Owens Street, San Francisco, CA 94158, United States
    Semin Cell Dev Biol 18:132-9. 2007
    ..Here, we review the known genetic causes of cardiac malformations and discuss future approaches for addressing sporadic congenital heart disease as a complex trait...
  64. doi request reprint Stem cells in the land of the rising sun: ISSCR 2012
    Kathrin Plath
    Department of Biological Chemistry, Jonsson Comprehensive Cancer Center, Molecular Biology Institute, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90024, USA
    Cell Stem Cell 11:607-14. 2012
    ..Held in Japan, the meeting showcased recent discoveries and surveyed the remarkable progress that has been made in a decade of stem cell research...
  65. pmc The paradoxical patent ductus arteriosus
    Kathryn N Ivey
    Gladstone Institute of Cardiovascular Disease, University of California San Francisco, San Francisco, California 94158, USA
    J Clin Invest 116:2863-5. 2006
    ....
  66. pmc Tbx1 is regulated by tissue-specific forkhead proteins through a common Sonic hedgehog-responsive enhancer
    Hiroyuki Yamagishi
    Department of Pediatrics, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9148, USA
    Genes Dev 17:269-81. 2003
    ..We propose that Tbx1 is a direct transcriptional target of Fox proteins and that Fox proteins may serve an intermediary role in Shh regulation of Tbx1...
  67. ncbi request reprint Galphaq and Galpha11 proteins mediate endothelin-1 signaling in neural crest-derived pharyngeal arch mesenchyme
    Kathryn Ivey
    Department of Molecular Biology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390 9104, USA
    Dev Biol 255:230-7. 2003
    ..We conclude that the Galpha(q) and Galpha(11) proteins serve as the intracellular mediators of ET-1 signaling in the pharyngeal arch mesenchyme...
  68. ncbi request reprint Tooth-type specific expression of dHAND/Hand2: possible involvement in murine lower incisor morphogenesis
    Makoto Abe
    Department of Oral Anatomy and Developmental Biology, Osaka University Graduate School of Dentistry, 1 8, Yamadaoka, Suita, Osaka 565 0871, Japan
    Cell Tissue Res 310:201-12. 2002
    ..The present results suggest that the dHAND gene plays important roles in type-specific development of lower incisors, and that basic fibroblast growth factor is involved downstream of the dHAND pathway in tooth germ cells...
  69. ncbi request reprint Bop encodes a muscle-restricted protein containing MYND and SET domains and is essential for cardiac differentiation and morphogenesis
    Paul D Gottlieb
    Section of Molecular Genetics and Microbiology and Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, Texas 78712, USA
    Nat Genet 31:25-32. 2002
    ..These results indicate that m-Bop is essential for cardiomyocyte differentiation and cardiac morphogenesis...
  70. ncbi request reprint Hairy-related transcription factors inhibit GATA-dependent cardiac gene expression through a signal-responsive mechanism
    Irfan S Kathiriya
    Department of Molecular Biology, The University of Texas Southwestern Medical Center, Dallas, Texas 75390 9148, USA
    J Biol Chem 279:54937-43. 2004
    ..These results suggest that HRT proteins may regulate specific sets of cardiac genes by modulating the function of GATA proteins and other cardiac transcriptional activators in a signal-dependent manner...
  71. ncbi request reprint Endotoxin-induced cardiomyopathy and systemic inflammation in mice is prevented by aldose reductase inhibition
    Kota V Ramana
    Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, TX 77555, USA
    Circulation 114:1838-46. 2006
    ..Postreceptor signaling events that lead to stress responses and cytokine production are sensitive to redox changes and products of lipid peroxidation...
  72. ncbi request reprint The Hand1 and Hand2 transcription factors regulate expansion of the embryonic cardiac ventricles in a gene dosage-dependent manner
    David G McFadden
    Department of Molecular Biology, University of Texas Southwestern Medical Center, 6000 Harry Hines Boulevard, Dallas, TX 75390 9148, USA
    Development 132:189-201. 2005
    ..These findings demonstrate that Hand factors play pivotal and partially redundant roles in cardiac morphogenesis, cardiomyocyte differentiation and cardiac-specific transcription...
  73. ncbi request reprint Serum response factor regulates a muscle-specific microRNA that targets Hand2 during cardiogenesis
    Yong Zhao
    Department of Pediatrics Cardiology, University of Texas Southwestern Medical Center and Children s Medical Center Dallas, 6000 Harry Hines Boulevard, Dallas, Texas 75390 9148, USA
    Nature 436:214-20. 2005
    ..This work suggests that miR-1 genes titrate the effects of critical cardiac regulatory proteins to control the balance between differentiation and proliferation during cardiogenesis...
  74. ncbi request reprint Cooperative and antagonistic interactions between Sall4 and Tbx5 pattern the mouse limb and heart
    Kazuko Koshiba-Takeuchi
    Programs in Cardiovascular Research, The Hospital for Sick Children, Toronto, Ontario M5G 1X8, Canada
    Nat Genet 38:175-83. 2006
    ..Thus, a positive and negative feed-forward circuit between Tbx5 and Sall4 ensures precise patterning of embryonic limb and heart and provides a unifying mechanism for heart/hand syndromes...
  75. pmc Essential roles of the bHLH transcription factor Hrt2 in repression of atrial gene expression and maintenance of postnatal cardiac function
    Mei Xin
    Department of Molecular Biology, University of Texas Southwestern Medical Center, 6000 Harry Hines Boulevard, Dallas, TX 75390, USA
    Proc Natl Acad Sci U S A 104:7975-80. 2007
    ..These findings reveal a ventricular myocardial cell-autonomous function for Hrt2 in the suppression of atrial cell identity and the maintenance of postnatal cardiac function...
  76. ncbi request reprint Genetic and comparative mapping of genes dysregulated in mouse hearts lacking the Hand2 transcription factor gene
    Melissa P Villanueva
    Department of Physiology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Genomics 80:593-600. 2002
    ..Nebulette was shown to be deleted in DiGeorge Syndrome 2 patients with the proximal deletion of human 10p13-p14 that is associated with cardiac and craniofacial abnormalities...
  77. ncbi request reprint Genetic basis for congenital heart defects: current knowledge: a scientific statement from the American Heart Association Congenital Cardiac Defects Committee, Council on Cardiovascular Disease in the Young: endorsed by the American Academy of Pediatrics
    Mary Ella Pierpont
    Children s Hospital of Minnesota and University of Minnesota, USA
    Circulation 115:3015-38. 2007
    ....
  78. pmc Spectrum of heart disease associated with murine and human GATA4 mutation
    Satish K Rajagopal
    Department of Cardiology, Children s Hospital Boston, 300 Longwood Avenue, Boston, MA 02115, USA
    J Mol Cell Cardiol 43:677-85. 2007
    ..Additional studies will be required to determine the degree to which GATA4 mutation contributes to human CHD characterized by ECD or RV hypoplasia...
  79. ncbi request reprint Pbx1/Pbx2 requirement for distal limb patterning is mediated by the hierarchical control of Hox gene spatial distribution and Shh expression
    Terence D Capellini
    Department of Cell and Developmental Biology, Cornell University Weill Medical School, New York, NY 10021, USA
    Development 133:2263-73. 2006
    ....
  80. pmc Tbx1 is regulated by forkhead proteins in the secondary heart field
    Jun Maeda
    Department of Pediatrics, Division of Pediatric Cardiology, Keio University School of Medicine, Tokyo, Japan
    Dev Dyn 235:701-10. 2006
    ....
  81. ncbi request reprint Heart disease: an ongoing genetic battle?
    Deepak Srivastava
    Nature 429:819-22. 2004
  82. pmc Vertebrate heart growth is regulated by functional antagonism between Gridlock and Gata5
    Haibo Jia
    Department of Medicine and Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    Proc Natl Acad Sci U S A 104:14008-13. 2007
    ..Hence, our studies suggest that Grl regulates embryonic heart growth via opposing Gata5, at least in part through their protein interactions in modulating gene expression...
  83. ncbi request reprint Ephrin-B2 reverse signaling is required for axon pathfinding and cardiac valve formation but not early vascular development
    Chad A Cowan
    Center for Developmental Biology and Kent Waldrep Center for Basic Research on Nerve Growth and Regeneration, University of Texas Southwestern Medical Center, Dallas, TX 75390 9133, USA
    Dev Biol 271:263-71. 2004
    ....
  84. pmc The homeodomain transcription factor Irx5 establishes the mouse cardiac ventricular repolarization gradient
    Danny L Costantini
    Program in Cardiovascular Research, The Hospital for Sick Children, Toronto, Ontario M5G 1X8, Canada
    Cell 123:347-58. 2005
    ..Thus, an Irx5 repressor gradient negatively regulates potassium-channel-gene expression in the heart, forming an inverse I(to,f) gradient that ensures coordinated cardiac repolarization while also preventing arrhythmias...

Research Grants32

  1. Cardiogenesis: Molecular Mechanisms
    Deepak Srivastava; Fiscal Year: 2010
    ..This work promises to uncover a new area of molecular regulation in the heart and may provide mechanisms to finely regulate dosages of critical proteins in the prenatal or postnatal heart. ..
  2. Isolated and Syndromic Cardiac Outflow Tract Defects
    Deepak Srivastava; Fiscal Year: 2006
    ..These studies will reveal the upstream and downstream mechanisms through which Tbx1 functions and are essential to understanding the pathogenesis of syndromic types of pharyngeal arch and cardiac outflow tract defects. ..
  3. CARDIOGENESIS-- MOLECULAR MECHANISMS
    Deepak Srivastava; Fiscal Year: 2005
    ..These studies will provide insights into the mechanisms of segmented gene regulation during cardiac morphogenesis that may ultimately allow for preventive interventions for CHD. ..
  4. Cardiogenesis: Molecular Mechanisms
    Deepak Srivastava; Fiscal Year: 2007
    ..This work promises to uncover a new area of molecular regulation in the heart and may provide mechanisms to finely regulate dosages of critical proteins in the prenatal or postnatal heart. ..
  5. Cardiac Defects/Cross-species analysis/Human Mutations
    Deepak Srivastava; Fiscal Year: 2009
    ..Such studies will not only provide insight into the relevance of the human mutations, but will also reveal important structure-function aspects regarding the biology through which the cardiac regulatory proteins operate. ..
  6. Cardiogenesis: Molecular Mechanisms
    Deepak Srivastava; Fiscal Year: 2009
    ..This work promises to uncover a new area of molecular regulation in the heart and may provide mechanisms to finely regulate dosages of critical proteins in the prenatal or postnatal heart. ..
  7. CARDIOGENESIS--MOLECULAR MECHANISMS
    Deepak Srivastava; Fiscal Year: 2000
    ..The third and final aim will define the cis-acting sequences that regulate the expression of the HAND genes in tissue culture cells and transgenic mice. ..
  8. SYNDROMIC CARDIAC OUTFLOW TRACT DEFECTS
    Deepak Srivastava; Fiscal Year: 2002
    ..In this fashion, we intend to approach the molecular basis for certain congenital heart defects, particularly those affecting the cardiac outflow tract and aortic arch in isolation and in CATCH-22 syndrome. ..
  9. Cardiac Development and Cogenital Heart Disease
    Deepak Srivastava; Fiscal Year: 2004
    ..The exposure of graduate students and post-doctoral fellows to the exciting opportunities in this field is essential for future progress and for the career development of the trainees. ..