Robert J Spreitzer

Summary

Affiliation: University of Nebraska
Country: USA

Publications

  1. pmc Plant-like substitutions in the large-subunit carboxy terminus of Chlamydomonas Rubisco increase CO2/O2 specificity
    Sriram Satagopan
    Department of Microbiology, Ohio State University, Columbus, OH 43210, USA
    BMC Plant Biol 8:85. 2008
  2. ncbi request reprint Alanine-scanning mutagenesis of the small-subunit beta A-beta B loop of chloroplast ribulose-1,5-bisphosphate carboxylase/oxygenase: substitution at Arg-71 affects thermal stability and CO2/O2 specificity
    R J Spreitzer
    Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588, USA
    Biochemistry 40:5615-21. 2001
  3. ncbi request reprint Rubisco: structure, regulatory interactions, and possibilities for a better enzyme
    Robert J Spreitzer
    Department of Biochemistry, Institute of Agriculture and Natural Resources, University of Nebraska, Lincoln, Nebraska 68588 0664, USA
    Annu Rev Plant Biol 53:449-75. 2002
  4. ncbi request reprint Role of the small subunit in ribulose-1,5-bisphosphate carboxylase/oxygenase
    Robert J Spreitzer
    Department of Biochemistry, University of Nebraska, N217 Beadle Center, Lincoln, NE 68588 0664, USA
    Arch Biochem Biophys 414:141-9. 2003
  5. pmc Phylogenetic engineering at an interface between large and small subunits imparts land-plant kinetic properties to algal Rubisco
    Robert J Spreitzer
    Department of Biochemistry, University of Nebraska, Lincoln, NE 68588 0664, USA
    Proc Natl Acad Sci U S A 102:17225-30. 2005
  6. ncbi request reprint Substitutions at the Asp-473 latch residue of chlamydomonas ribulosebisphosphate carboxylase/oxygenase cause decreases in carboxylation efficiency and CO(2)/O(2) specificity
    Sriram Satagopan
    Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588 0664, USA
    J Biol Chem 279:14240-4. 2004
  7. pmc Highly conserved small subunit residues influence rubisco large subunit catalysis
    Todor Genkov
    Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588, USA
    J Biol Chem 284:30105-12. 2009
  8. ncbi request reprint Small-subunit cysteine-65 substitutions can suppress or induce alterations in the large-subunit catalytic efficiency and holoenzyme thermal stability of ribulose-1,5-bisphosphate carboxylase/oxygenase
    Todor Genkov
    Department of Biochemistry, University of Nebraska, Lincoln, NE 68588 0664, USA
    Arch Biochem Biophys 451:167-74. 2006
  9. pmc Functional hybrid rubisco enzymes with plant small subunits and algal large subunits: engineered rbcS cDNA for expression in chlamydomonas
    Todor Genkov
    Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588, USA
    J Biol Chem 285:19833-41. 2010
  10. ncbi request reprint Assessment of structural and functional divergence far from the large subunit active site of ribulose-1,5-bisphosphate carboxylase/oxygenase
    Yu Chun Du
    Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588 0664, USA
    J Biol Chem 278:49401-5. 2003

Collaborators

Detail Information

Publications15

  1. pmc Plant-like substitutions in the large-subunit carboxy terminus of Chlamydomonas Rubisco increase CO2/O2 specificity
    Sriram Satagopan
    Department of Microbiology, Ohio State University, Columbus, OH 43210, USA
    BMC Plant Biol 8:85. 2008
    ....
  2. ncbi request reprint Alanine-scanning mutagenesis of the small-subunit beta A-beta B loop of chloroplast ribulose-1,5-bisphosphate carboxylase/oxygenase: substitution at Arg-71 affects thermal stability and CO2/O2 specificity
    R J Spreitzer
    Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588, USA
    Biochemistry 40:5615-21. 2001
    ..Despite the fact that Arg-71 is more than 20 A from the large-subunit active site, it is apparent that the small-subunit betaA-betaB loop region can influence catalytic efficiency and CO2/O2 specificity...
  3. ncbi request reprint Rubisco: structure, regulatory interactions, and possibilities for a better enzyme
    Robert J Spreitzer
    Department of Biochemistry, Institute of Agriculture and Natural Resources, University of Nebraska, Lincoln, Nebraska 68588 0664, USA
    Annu Rev Plant Biol 53:449-75. 2002
    ....
  4. ncbi request reprint Role of the small subunit in ribulose-1,5-bisphosphate carboxylase/oxygenase
    Robert J Spreitzer
    Department of Biochemistry, University of Nebraska, N217 Beadle Center, Lincoln, NE 68588 0664, USA
    Arch Biochem Biophys 414:141-9. 2003
    ..It is thus apparent that small subunits will need to be taken into account as strategies are developed for creating better Rubisco enzymes...
  5. pmc Phylogenetic engineering at an interface between large and small subunits imparts land-plant kinetic properties to algal Rubisco
    Robert J Spreitzer
    Department of Biochemistry, University of Nebraska, Lincoln, NE 68588 0664, USA
    Proc Natl Acad Sci U S A 102:17225-30. 2005
    ..Thus, this interface between large and small subunits, far from the active site, contributes significantly to the differences in catalytic properties between algal and land-plant Rubisco enzymes...
  6. ncbi request reprint Substitutions at the Asp-473 latch residue of chlamydomonas ribulosebisphosphate carboxylase/oxygenase cause decreases in carboxylation efficiency and CO(2)/O(2) specificity
    Sriram Satagopan
    Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588 0664, USA
    J Biol Chem 279:14240-4. 2004
    ..Considering that substitutions at residue 473 can influence CO(2)/O(2) specificity, further study of interactions between loop 6 and the carboxyl terminus may provide clues for engineering an improved Rubisco...
  7. pmc Highly conserved small subunit residues influence rubisco large subunit catalysis
    Todor Genkov
    Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588, USA
    J Biol Chem 284:30105-12. 2009
    ....
  8. ncbi request reprint Small-subunit cysteine-65 substitutions can suppress or induce alterations in the large-subunit catalytic efficiency and holoenzyme thermal stability of ribulose-1,5-bisphosphate carboxylase/oxygenase
    Todor Genkov
    Department of Biochemistry, University of Nebraska, Lincoln, NE 68588 0664, USA
    Arch Biochem Biophys 451:167-74. 2006
    ..Further analysis of the C65P protein may identify structural alterations that influence catalysis separate from those that affect stability...
  9. pmc Functional hybrid rubisco enzymes with plant small subunits and algal large subunits: engineered rbcS cDNA for expression in chlamydomonas
    Todor Genkov
    Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588, USA
    J Biol Chem 285:19833-41. 2010
    ..It appears that small subunits contain the structural elements responsible for targeting Rubisco to the algal pyrenoid, which is the site where CO(2) is concentrated for optimal photosynthesis...
  10. ncbi request reprint Assessment of structural and functional divergence far from the large subunit active site of ribulose-1,5-bisphosphate carboxylase/oxygenase
    Yu Chun Du
    Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588 0664, USA
    J Biol Chem 278:49401-5. 2003
    ..The Rubisco x-ray crystal structures indicate that these residues may reside in a variable loop of the nuclear-encoded small subunit, more than 20 A away from the active site...
  11. ncbi request reprint Altered intersubunit interactions in crystal structures of catalytically compromised ribulose-1,5-bisphosphate carboxylase/oxygenase
    Saeid Karkehabadi
    Department of Molecular Biology, Swedish University of Agricultural Sciences, BMC Box 590, 751 24 Uppsala, Sweden
    Biochemistry 44:113-20. 2005
    ..This suggests that alterations in subunit interactions will influence protein dynamics and, thereby, affect catalysis...
  12. ncbi request reprint Structural analysis of altered large-subunit loop-6/carboxy-terminus interactions that influence catalytic efficiency and CO2/O2 specificity of ribulose-1,5-bisphosphate carboxylase/oxygenase
    Saeid Karkehabadi
    Department of Molecular Biology, Swedish University of Agricultural Sciences, BMC Box 590, 751 24 Uppsala, Sweden
    Biochemistry 46:11080-9. 2007
    ..However, active-site Lys334 at the apex of loop 6 has a normal conformation. A variety of subtle interactions must be responsible for catalytic efficiency and CO2/O2 specificity...
  13. ncbi request reprint Structural and functional consequences of the replacement of proximal residues Cys(172) and Cys(192) in the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase from Chlamydomonas reinhardtii
    María Jesús García-Murria
    Departament de Bioquimica i Biologia Molecular, Facultat de Biologia, Universitat de Valencia, Av Dr Moliner 50, Burjassot, València E 46100, Spain
    Biochem J 411:241-7. 2008
    ..This may cause the exceptional catalytic features of C172S. In contrast, the C192S mutation does not produce similar structural perturbations...
  14. ncbi request reprint Deletion of nine carboxy-terminal residues of the Rubisco small subunit decreases thermal stability but does not eliminate function
    Maria G Esquível
    Department of Botany and Biological Engineering, Technical University of Lisbon, P 1399 Lisbon, Portugal
    FEBS Lett 520:73-6. 2002
    ..Thus, although disordered or divergent, the carboxy terminus is required for maximal activity and stability...
  15. ncbi request reprint Chimeric small subunits influence catalysis without causing global conformational changes in the crystal structure of ribulose-1,5-bisphosphate carboxylase/oxygenase
    Saeid Karkehabadi
    Department of Molecular Biology, Swedish University of Agricultural Sciences, 751 24 Uppsala, Sweden
    Biochemistry 44:9851-61. 2005
    ..This loop may be a worthwhile target for genetic engineering aimed at improving photosynthetic CO(2) fixation...